8 results about "Prednisolone" patented technology

The invention relates to a preparation method of prednisone acetate. The preparation method comprises the following steps: carrying out secondary culture on arthrobacter simplex; adding a crude product of cortisone acetate into the arthrobacter simplex subjected to secondary culture, performing fermentation conversion, and performing conversion by adopting a mode of gradient temperature control and batch addition of an organic solvent to obtain a crude product of prednisone acetate; refining the prednisone acetate crude product, pulping with ethyl acetate, heating and refluxing, and repeatedly refining for multiple times to obtain a prednisone acetate refined product; and heating and dissolving the prednisone acetate refined product with ethyl acetate, adding activated carbon and water, heating and refluxing, filtering and concentrating to obtain the prednisone acetate refined product. According to the method disclosed by the invention, a mode of gradient temperature control and batch addition of the organic solvent is adopted during conversion, so that the strain conversion efficiency is improved, and the conversion rate of prednisone acetate reaches 94% or above; during refining, a pulping mode is adopted, so that the batch feeding amount is increased, a repeated refining and concentrating process is removed, the solvent consumption is reduced, and the process time is greatly shortened.

The application provides a culture method and composition for prolonging the in-vitro survival time of neutrophils. The composition provided by the application comprises prednisolone and beta-mercaptoethanol, which can prolong the survival time of neutrophils. The method provided by the application has low cost, simple operation, small interference to cell function, can guarantee an experimental window of 3-4 days, and has good application value.

The present disclosure provides ophthalmic compositions comprising a steroid, such as prednisolone encapsulated in a liposomal carrier and methods of use thereof.

The invention relates to the technical field of biology, in particular to a dissociation agent and a kit for progesterone determination. According to the invention, the sample dissociation agent for progesterone detection is obtained through optimized screening, and the sample dissociation agent comprises hydrocortisone, prednisolone and mono (2-ethylhexyl) phthalate (MEHP); when the dissociation agent components act independently or in a combined mode, the sensitivity to sample detection is high, the detection linear range is good, the dissociation agent is well related to a comparison reagent, the dissociation effect is improved remarkably on the whole, progesterone can be better released from corticosteroid binding protein, albumin and sex hormone binding globulin, and the progesterone dissociation agent is suitable for being used for detecting progesterone. The combination of progesterone and a captured antibody is facilitated, and the sensitivity and the accuracy can be effectively improved. Moreover, the reagent can be directly added into reagent components, so that the dissociation process and the immune reaction are carried out at the same time, the immune reaction is simple and efficient, and the reaction efficiency is improved.

The application discloses application of water extract of Euryale ferox in preparation of food and medicine for improving osteoporosis, and relates to the technical field of natural medicine. The raw Euryale ferox, salt-fried Euryale ferox and bran-fried Euryale ferox are respectively used as raw materials, and the water extract of Euryale ferox is prepared by using a water extraction process after standard cleaning and processing. A double pharmacodynamic evaluation system of a prednisolone-induced zebra fish skull osteoporosis in vivo model and an MC3T3-E1 cell osteogenic differentiation in vitro model is established, and the in-vivo anti-osteoporosis effect, embryonic development safety, in-vitro cytotoxicity and osteogenic differentiation activity of the water extract of Euryale ferox with different processing methods and different concentrations are systematically investigated. The salt-fried Euryale ferox has better pharmacodynamic effect than the raw Euryale ferox and the bran-fried Euryale ferox under the same concentration. The application provides a scientific basis for the application of Euryale ferox in the development of anti-osteoporosis drugs.

This invention discloses a quantitative analysis method for the blood concentrations of HYQ-169 and prednisolone and its application, belonging to the fields of medical testing and pharmaceutical analysis. The quantitative analysis method includes the following steps: preparing standard curve samples and quality control samples; adding internal standard solution; extracting with ethyl acetate; drying the supernatant and reconstituteing; separating using SPE; drying the fraction and reconstituteing again; detecting using UPLC-MS / MS; establishing a standard curve and obtaining a regression equation; adding internal standard solution to the plasma sample to be tested and processing it in the same way; calculating the blood concentrations of HYQ-169 and prednisolone according to the regression equation. This invention can simultaneously perform quantitative detection of two drug components with significantly different chemical properties in a single analysis, with high sensitivity, high accuracy, and fast analysis speed, providing a foundation for preclinical and clinical pharmacokinetic studies of HYQ-169.

The present disclosure provides ophthalmic compositions comprising a steroid, such as prednisolone, encapsulated in a liposomal carrier, and methods of using the same.

This invention discloses a method for simultaneously detecting cefepime and its metabolites, as well as prednisolone residues in milk. The method includes the following steps: taking a milk sample, extracting with acetonitrile, centrifuging, and collecting the supernatant; adding acetonitrile-saturated n-hexane for liquid-liquid extraction to remove fat; concentrating the extracted solution, reconstituted with phosphate buffer at pH 8.5 ± 0.2, purifying with a hydrophilic-lipophilic equilibrium solid-phase extraction column, eluting, concentrating, and reconstituted to obtain the test solution; and detecting the test solution using ultra-high performance liquid chromatography-tandem mass spectrometry (UHPLC-MS / MS). This invention, through optimized pretreatment processes, particularly the dual purification strategy of "n-hexane degreasing + HLB solid-phase extraction" combined with precise pH adjustment, effectively removes complex fat and protein matrix interferences in milk, significantly reducing matrix effects; combined with optimized gradient elution procedures and multiple reaction monitoring (MRM) mass spectrometry conditions, it achieves simultaneous, rapid, and highly sensitive detection of cefepime, deacetylated cefepime, and prednisolone.