5 results about "Prednisolone" patented technology

The present disclosure provides ophthalmic compositions comprising a steroid, such as prednisolone encapsulated in a liposomal carrier and methods of use thereof.

The application discloses application of water extract of Euryale ferox in preparation of food and medicine for improving osteoporosis, and relates to the technical field of natural medicine. The raw Euryale ferox, salt-fried Euryale ferox and bran-fried Euryale ferox are respectively used as raw materials, and the water extract of Euryale ferox is prepared by using a water extraction process after standard cleaning and processing. A double pharmacodynamic evaluation system of a prednisolone-induced zebra fish skull osteoporosis in vivo model and an MC3T3-E1 cell osteogenic differentiation in vitro model is established, and the in-vivo anti-osteoporosis effect, embryonic development safety, in-vitro cytotoxicity and osteogenic differentiation activity of the water extract of Euryale ferox with different processing methods and different concentrations are systematically investigated. The salt-fried Euryale ferox has better pharmacodynamic effect than the raw Euryale ferox and the bran-fried Euryale ferox under the same concentration. The application provides a scientific basis for the application of Euryale ferox in the development of anti-osteoporosis drugs.

This invention discloses a quantitative analysis method for the blood concentrations of HYQ-169 and prednisolone and its application, belonging to the fields of medical testing and pharmaceutical analysis. The quantitative analysis method includes the following steps: preparing standard curve samples and quality control samples; adding internal standard solution; extracting with ethyl acetate; drying the supernatant and reconstituteing; separating using SPE; drying the fraction and reconstituteing again; detecting using UPLC-MS / MS; establishing a standard curve and obtaining a regression equation; adding internal standard solution to the plasma sample to be tested and processing it in the same way; calculating the blood concentrations of HYQ-169 and prednisolone according to the regression equation. This invention can simultaneously perform quantitative detection of two drug components with significantly different chemical properties in a single analysis, with high sensitivity, high accuracy, and fast analysis speed, providing a foundation for preclinical and clinical pharmacokinetic studies of HYQ-169.

This invention discloses a method for simultaneously detecting cefepime and its metabolites, as well as prednisolone residues in milk. The method includes the following steps: taking a milk sample, extracting with acetonitrile, centrifuging, and collecting the supernatant; adding acetonitrile-saturated n-hexane for liquid-liquid extraction to remove fat; concentrating the extracted solution, reconstituted with phosphate buffer at pH 8.5 ± 0.2, purifying with a hydrophilic-lipophilic equilibrium solid-phase extraction column, eluting, concentrating, and reconstituted to obtain the test solution; and detecting the test solution using ultra-high performance liquid chromatography-tandem mass spectrometry (UHPLC-MS / MS). This invention, through optimized pretreatment processes, particularly the dual purification strategy of "n-hexane degreasing + HLB solid-phase extraction" combined with precise pH adjustment, effectively removes complex fat and protein matrix interferences in milk, significantly reducing matrix effects; combined with optimized gradient elution procedures and multiple reaction monitoring (MRM) mass spectrometry conditions, it achieves simultaneous, rapid, and highly sensitive detection of cefepime, deacetylated cefepime, and prednisolone.