7 results about "Ascomycin" patented technology

This invention proposes a purification method for ascomycin and its application. The method includes acidifying and filtering the ascomycin fermentation broth to obtain a bacterial residue; adding organic solvent I to the bacterial residue for extraction to obtain an extract filtrate; sequentially adding a salt solution, an alkaline solution, and an acid solution to the extract filtrate for treatment, and decolorizing the product to obtain a decolorized organic phase; evaporating the decolorized organic phase under reduced pressure, and sequentially treating it with ethyl acetate and n-hexane, followed by cooling and filtration to obtain wet crystals 1, which are then washed with n-hexane to obtain wet crystals 2; drying the wet crystals 2 sequentially, dissolving them in organic solvent II to obtain a pre-crystallization liquid, crystallizing by low-temperature stirring, filtering, and then vacuum drying the solid phase. This method can obtain ascomycin with high purity (not less than 99.2%), a yield higher than 61%, and is simple to operate, easy to scale up for industrial production, and has low production costs, making it highly valuable for application.

The application discloses a method for simultaneously and rapidly detecting the concentration of four immunosuppressants, comprising the following steps: mixing a blood sample with an internal standard solution containing ascomycin, cyclosporine-d4, sirolimus-d3 and everolimus-d4, adding a precipitant and centrifuging to obtain a sample to be detected; injecting into a liquid chromatography-tandem mass spectrometry system for analysis to obtain original data containing the retention time window and mass spectrum response signal of four analytes; searching the original data based on an interferent database, wherein the interferent database contains a plurality of compounds that can possibly generate mass spectrum signal interference and characteristic ion information thereof; inputting the original data and the searched potential interferent information into a machine learning model to output a net mass spectrum response value after interference correction; and calculating the concentration of tacrolimus, cyclosporine, sirolimus and everolimus in the blood sample according to the obtained net mass spectrum response value, the mass spectrum response value of the corresponding internal standard and a pre-established standard curve.

This invention discloses a method for extracting, separating, and purifying ascomycin from mycelia. The method combines extraction, macroporous resin adsorption, and reverse polymer chromatography to separate ascomycin and its main impurity, demethylascomycin, from mycelia, ultimately yielding ascomycin with a purity of over 98%. This method exhibits high impurity removal rate and excellent separation effect. It has significant advantages in the pharmaceutical field and is suitable for industrial application.

The present invention provides compositions and methods for the treatment or prevention of pulmonary hypertension comprising administering an ascomycin, or a pharmaceutically acceptable salt, solvate, analog, or prodrug thereof to the patient with pulmonary hypertension.

According to the tacrolimus fermentation preparation process provided by the invention, by optimizing a fermentation culture medium formula and a fermentation culture process, adding L-arginine and a trace element solution, controlling the content of dissolved oxygen and adjusting the metabolic pathway of production bacteria, the metabolism tends to generate tacrolimus, the generation of ascomycin is reduced, and the production efficiency is improved. The ascomycin content in the fermentation liquor is greatly reduced, so that the cost of downstream refining production is reduced.

The invention provides a method for separating and purifying ascomycin. The method for separating and purifying ascomycin comprises the following steps: 1) adding inorganic salt into fermentation liquor containing ascomycin, adjusting the pH value of a system to be 1-4, and carrying out first ceramic membrane filtration to obtain first ceramic membrane concentrated liquor; adding alkaline buffer salt into the first ceramic membrane concentrated solution, adjusting the pH value of the system to 8-11, and carrying out second ceramic membrane filtration to obtain a second ceramic membrane concentrated solution; (2) carrying out filter pressing and water washing on the second ceramic membrane concentrated solution to obtain a filter cake, and extracting the filter cake by using an organic solvent to obtain an extracting solution; (3) cooling, crystallizing, filtering and drying the extract liquor to obtain first purified crystallis.By means of the method, cross-linked impurities such as amino acid, protein and pigment in the fermentation liquor can be effectively removed, and the effect of efficiently separating and purifying high-purity ascomycin is achieved.

The present invention provides compositions and methods for the treatment or prevention of pulmonary hypertension comprising administering an ascomycin, or a pharmaceutically acceptable salt, solvate, analog, or prodrug thereof to the patient with pulmonary hypertension. Described herein are liquid formulations which deliver an ascomycin. The liquid formulation can be placed in a soft gelatin capsule.