7 results about "Mutation rate" patented technology

The purpose of this invention is to provide a method for detecting mutant genes that can comprehensively and without omission measure the mutation rate of genes while suppressing costs. The method for detecting mutant genes of this invention involves performing a base extension reaction using a first pigment group and a second pigment group, respectively. The first pigment group is configured such that one of the bases A and B is not fluorescently labeled, while the other is fluorescently labeled, and GC is fluorescently labeled. The second pigment group is configured such that one of the bases C is not fluorescently labeled, while the other is fluorescently labeled, and AT is fluorescently labeled. Both the first and second pigment groups are configured such that the base extension reaction does not stop at the unlabeled bases but stops at the fluorescently labeled bases (see Figure 6).

The application discloses a construction method of a glycogen storage disease type 3 (GSD III) zebrafish F0 generation model and application thereof in drug screening, mixes sgRNA combinations with Cas9 protein, and jointly injects into single-cell period embryos of wild type zebrafish to cultivate and obtain F0 generation zebrafish models with double allelic mutations. The sgRNA combinations comprise a first targeting sequence and a second targeting sequence for an agla gene, and a third targeting sequence and a fourth targeting sequence for an aglb gene. The application breaks through the technical barrier that a traditional GSD III zebrafish double knockout homozygous strain construction cycle lasts for 6-12 months, through screening of high-activity sgRNA combinations and a high-concentration Cas9 protein co-injection strategy in the single-cell period, the application realizes a very high double allelic mutation rate in the F0 generation, and effectively overcomes the defects of high chimerism and unstable phenotype in the traditional F0 generation.

This invention discloses a method and its application for creating stress-resistant mutants of common bean using chemical mutagenesis. The main steps include: determining the screening concentration of hydroxyproline (Hyp), determining the median lethal dose of ethyl methanesulfonate (EMS), and subculturing the stress-resistant mutants. After 3-5 subcultures, the stress-resistant mutants screened by this invention ultimately yielded 31 cotyledonary nodes of common bean resistant to hydroxyproline (Hyp), with a mutation rate of 8.16%. This invention can induce mutagenesis and directionally screen for stress-resistant common bean mutants, providing material support for stress-resistant breeding of common bean.

The application provides a network updating driven adaptive genetic clustering optimization method, device and medium, and belongs to the field of unmanned underwater vehicle cluster cooperative control.The method takes the current position and residual energy of the AUV as input, adopts individual coding composed of cluster head vector I and membership vector M, calculates the relative position change according to the node motion state at each network update, adaptively sets the mutation rate, and performs multi-generation genetic optimization within a single network update.The fitness function comprehensively considers the communication distance, cluster head energy, cluster size uniformity and cluster head energy balance;in the selection stage, the elite reservation and roulette are combined;in the crossover stage, the dynamic crossover rate is adopted with the generation number decreasing;in the mutation stage, the exchange mutation and constraint repair mechanism are adopted;at the same time, the excellent individuals of the last network update are inherited and random individuals are injected, so that the solving efficiency and stability under the dynamic topology are improved.

The application provides a mutagenesis breeding method based on seed island Porphyra and application thereof, and belongs to the technical field of breeding.The mutagenesis breeding method based on seed island Porphyra provided by the application comprises the following steps: selecting immature seed island Porphyra thalli for segmentation to obtain segmented leaves; the segmented leaves are subjected to ARTP mutagenesis, the leaves after mutagenesis are subjected to light-avoiding culture, and then mechanical crushing is performed to 20-40 cell sizes, and then the mutants are screened.The application combines the single spore dissemination technology induced by damage and the ARTP technology, uses the seed island Porphyra thalli as mutagenesis materials for mutagenesis, greatly shortens the mutagenesis period and reduces the workload, has the advantages of high efficiency, high mutation rate and good specificity, and can obtain stable mutants.