374 results about "Gene code" patented technology

Certain C. glutamicum strains overexpress genes coding for enzymes having the function of aspartate-semialdehyde dehydrogenase, aspartate aminotransferase, dihydrodipicolinate synthase, dihydrodipicolinate reductase, diaminopimelate decarboxylase, aspartatokinase and diaminopimelate dehydrogenase. The C. glutamicum strains also express NAD(P)(+) transhydrogenase subunit alpha PntA and NAD(P)(+) transhydrogenase subunit beta PntB of Corynebacterium urealyticum. A method is developed for the fermentative production of L-lysine using such C. glutamicum strains.

The invention discloses application of wheat Rht1-D1b protein in regulation and control of a wheat tillering angle, and belongs to the technical field of biological breeding. The technical problem to be solved by the invention is how to increase the tillering angle of plants. Therefore, the invention provides the application of the protein or a substance for regulating and controlling the expression of a protein coding gene or a substance for regulating and controlling the activity or content of the protein in regulating and controlling the tillering angle of the plant, wherein the protein can be Rht1-D1b protein; the Rht1-D1b protein can be a protein of which the amino acid sequence is as shown in SEQ ID No. 3. According to the application, the Rht1-D1b gene is finally localized through gene localization and map-based cloning. The gene significantly increases the tillering angle of wheat. The wheat gene Rht1-D1b can be widely applied to the plant fields of wheat germplasm resource improvement, wheat plant type genetic breeding and the like, and has an important application value for improving the wheat yield.

The invention provides application of a P8 gene, or a P8 gene coding protein, or a recombinant vector or host bacteria containing a P8 gene coding region in improvement of GFP (Green Fluorescent Protein) expression quantity and / or inhibition of degradation of an mRNA (Messenger Ribonucleic Acid) attenuated substrate taking GFP as a reporter gene. The research finds that the P8 has a silence inhibition function, can inhibit degradation of a nonsense mediated mRNA decay substrate taking GFP as a reporter gene and degradation of an endogenous nonsense mediated mRNA decay (NMD) substrate of arabidopsis thaliana, can improve the mRNA expression quantity of AT1G0106020, RPS6, SMG7, AT5G35490, AT5G64430, AT1G36730, AT4G3900 and AT5G22570, and can reduce the expression quantity of AT1G72450 and AT2G400, and the research result lays a foundation for clarification of the function of the P8.

The invention discloses a corn ZmMYBR37 gene and an application of the corn ZmMYBR37 gene in regulating and controlling the size of corn kernels. The nucleotide sequence of the ZmMYBR37 gene is as shown in SEQ ID No: 1 in a sequence table. A mutant material with small grains and reduced hundred-grain weight is separated from a corn EMS mutant library, and a mutation site occurs on a function unknown gene ZmMYBR37, so that protein translation is terminated in advance. The information analysis finds that the gene coding protein has a conservative MYB structural domain, and the gene coding protein is speculated to belong to an MYB transcription factor family. Gene expression analysis finds that the gene is high in constitutive expression in corn and high in expression level in leaves and embryos. The corn material overexpressed with the ZmMYBR37 has the advantages that the grain size is increased, and the hundred-grain weight is obviously increased, so that the ZmMYBR37 has the potential of increasing the corn yield.

The invention belongs to the field of intelligent medical treatment, and particularly relates to a construction method of a virus single-gene host adaptability prediction model, equipment and a medium. The method comprises the steps that a training set virus single gene data set and a host tag are obtained, single genes in the virus single gene data set are sequentially encoded to obtain an encoding feature matrix set, and encoding comprises the steps that a sliding window method is used for traversing a gene sequence, frequencies of 64 different codons in a window form a window codon frequency vector, and the window codon frequency vector is used for encoding the host tag; representing a central codon of a window by using the window codon frequency vector, and representing each codon of a gene sequence as a window codon frequency vector in sequence; and inputting the coding feature matrix set into a residual network model to obtain a prediction label, and obtaining a single-gene host adaptability prediction model based on comparison iteration training of the prediction label and a host label. According to the method, the model capable of predicting the gene adaptability is obtained by training after the codon is represented, and the gene codon adaptability can be analyzed.

The invention relates to a diacylglycerol acyltransferase capable of synthesizing triglyceride as well as a coding gene and application of the diacylglycerol acyltransferase, and belongs to the field of enzymology, the nucleotide sequence of the gene for coding the diacylglycerol acyltransferase is as shown in SEQ ID NO.1, and the amino acid sequence of the diacylglycerol acyltransferase is as shown in SEQ ID NO.2. The invention also provides a recombinant plasmid and a recombinant engineering bacterium containing the gene of the enzyme. Diglyceride and an acyl donor are used as substrates, and triglyceride is catalytically synthesized through an acyl transfer reaction by using the enzyme. The invention provides an efficient tool for realizing enzymatic biosynthesis and large-scale production of natural triglyceride.

The invention provides an application of an AC2 gene, or an AC2 gene coding protein, or a recombinant vector or host bacteria containing an AC2 gene coding region in reducing the expression level of AtSGS3, and / or reducing the expression level of MeSGS3, and / or reducing an SGS3 / RDR6 compound. Researches find that SLCMV AC2 interacts with AtSGS3 and MeSGS3, the growth ability of yeast in an SD / -Ade / -His / -Leu / -Trp auxotroph culture medium and an SD / -Ade / -His / -Leu / -Trp auxotroph culture medium added with 3-AT can be improved, expression of AtSGS3 and MeSGS3 can be down-regulated, an SGS3 / RDR6 compound can be reduced, and a research result lays a foundation for clarification of a molecular mechanism of SLCMV AC2 regulation and control of host RNA silencing.

This invention provides a method for the in vivo delivery of oligonucleotides. The invention utilizes the presence of one or plurality of HES linked to an oligonucleotide to deliver a nucleic acid sequence of interest into the cytoplasm of cells and tissues of live organisms. The delivery vehicle is nontoxic to cells and organisms. Since delivery is sequence-independent and crosses membranes in a receptor-independent manner, the delivered oligonucleotide can target complementary sequences in the cytoplasm as well as in the nucleus of live cells. Sequences of bacterial or viral origin can also be targeted. The method can be used for delivery of genes coding for expression of specific proteins, antisense oligonucleotides, siRNAs, shRNAs, Dicer substrates, miRNAs, anti-miRNAs or any nucleic acid sequence in a living organism. The latter include mammals, plants, and microorganisms such as bacteria, protozoa, and viruses.

The invention provides an Escherichia coli gene modification method for improving the proportion of plasmid superhelix monomers and a product and application thereof, the method comprises the following steps: knocking out two DNA sequences in a genome of host Escherichia coli by adopting a CRISPR / Cas9 gene editing system, the DNA sequences comprising a recA + gene coding sequence and a recF + gene coding sequence; the nucleotide sequence from the 5'end to the 3 'end of the recA + gene coding sequence is as shown in SEQ ID NO.1, and the nucleotide sequence from the 5' end to the 3 'end of the recF + gene coding sequence is as shown in SEQ ID NO.2. When the escherichia coli engineering strain prepared by the method is used for producing plasmids, the generation of plasmid polymers can be remarkably reduced, and the superhelix monomer proportion of the plasmids can be increased.

The invention relates to the technical field of storage garbage recycling algorithms, in particular to a garbage recycling system based on a genetic algorithm, which comprises an environment sensing module, a dynamic excitation pool module, a gene coding module, a genetic operation module, a strategy evaluation module, a cold and hot data verification bridge module, a wear leveling compensator and an iterative deployment module. The environment sensing module is used for scene feature extraction and load mode classification. Through dynamic parameter optimization and a multi-scene adaptation mechanism, an optimal strategy parameter combination is automatically searched by utilizing a genetic algorithm, and controllable disturbance is introduced by dynamically adjusting sample weight and polynomial variation in combination with an entropy weight method, so that multi-target balance of write amplification, block efficiency and wear balance is realized; meanwhile, through a cold and hot data verification bridge and a wear leveling compensator module, the data state transition trend is predicted, parameter deviation is actively corrected, high robustness and long-term stability can still be kept in a complex load scene, and finally a closed-loop self-adaptive garbage collection strategy generation system is formed.

The invention provides an SNP (Single Nucleotide Polymorphism) site which is located at the 296th site of a MeGRXC4 gene coding region, the basic group is G or C, and the sequence of the MeGRXC4 gene coding region is as shown in SEQ ID NO: 1 or SEQ ID NO: 2. The SNP located at the 296th site of the MeGRXC4 nucleotide sequence is identified to be closely associated with the drought resistance of the cassava from the big data level for the first time, detection is facilitated, accuracy and reliability are achieved, operation is convenient, the drought resistance of the cassava is identified through the SNP sites, the accuracy is high, the method is not affected by the growth period of the cassava, selection can be conducted in the seedling period, the breeding efficiency is remarkably improved, and the method is suitable for large-scale popularization and application. In addition, when the basic group of the SNP site is G, MeGRXC4 can significantly improve the drought resistance of cassava, and the invention has important significance for revealing the relationship between allele selection and drought resistance character formation in the domestication process of cultivated cassava, and provides gene resources and scientific basis for cultivating new varieties of drought-resistant high-yield cassava by using key genes.

The male fertility restoration plant of the Solanaceae family is deficient or suppressed in the function of at least one of the fertility restoring genes (a)-(o). The fertility restoring genes (a)-(o) are genes coding for a protein or the like containing an amino acid sequence represented by any one of SEQ ID NOs: 1-5.

The invention relates to the technical field of goat molecular marker breeding, in particular to an SNP (Single Nucleotide Polymorphism) molecular marker for identifying or assisting in identifying the height of a goat body type and application of the SNP molecular marker. The SNP molecular marker provided by the invention is located at the 937th basic group of an SHBG gene coding region and has A / G polymorphism, and the login number of the SHBG gene is NC030826.1. According to the invention, a Leizhou goat is taken as an experimental material, sequencing analysis is carried out on an SHBG gene, A-G mutation exists at the 937th basic group in an SHBG gene coding region and is non-synonymous mutation, the mutation leads to mutation of amino acid from arginine to glycine, and the genotype of the site is related to the high and short foot character of the goat (when the genotype is GG or AG, the goat is a tall goat; and when the genotype is AA, the goat is a short type goat), the method can be used for identifying or assisting in identifying the height of the goat body type, and has important significance on breeding of goat varieties.

The present disclosure provides a method for producing a polyisoprenoid, which makes it possible to synthesize in vitro a polyisoprenoid having an unprecedented structure, such as a 100% cis-polyisoprenoid or a polyisoprenoid containing an allylic diphosphate derivative as an initiating terminal. The present disclosure relates to a method for producing a polyisoprenoid in vitro, which employs a gene coding for a neryl diphosphate synthase and rubber particles bound to a protein encoded by the gene, or a method for producing a polyisoprenoid, which includes introducing into a plant a vector in which a gene coding for a neryl diphosphate synthase is linked to a promoter having a promoter activity that drives laticifer-specific gene expression to express a protein encoded by the gene specifically in laticifers.

The embodiment of the invention relates to a processing method and device for a tRNA (transfer ribonucleic acid) adaptation index weight prediction model. The method comprises the following steps: constructing the adaptation index weight prediction model and three downstream task models; training the four models based on a target species data set; after training is finished, experimental conditions, genome sequences and target genes input by a user are preprocessed, and corresponding experimental condition coding vectors, genome sequence coding vectors, tRNA gene copy number vectors and target gene codon index sets are obtained; predicting by an adaptive index weight prediction model according to the genome sequence coding vector, the experimental condition coding vector and the tRNA gene copy number vector to obtain an adaptive index weight vector; and the transcription efficiency, the translation efficiency and the gene abundance of the target gene are predicted by the three downstream task models according to the adaptation index weight vector and the target gene codon index set respectively. According to the invention, the species suitability and prediction stability can be improved.

The invention relates to the technical field of crop genetic breeding, and particularly discloses a rice OsNRAMP5 gene mutant and application thereof. According to the rice OsNRAMP5 gene mutant, a wild rice OsNRAMP5 gene coding sequence is used as a reference sequence, and the mutant comprises mutations that the 804th G base is mutated into A, the 806th to 952nd bases are deleted and / or the 984th T base is mutated into C. The invention provides a novel rice two-line sterile line cadmium low-accumulation mutant, which enriches low-cadmium accumulation related genes and related mutants, and provides a novel method for creating low-cadmium accumulation rice two-line sterile line germplasm.

There is disclosed a mutant, non-naturally occurring or transgenic Nicotiana plant leaf or part of the plant leaf having modulated expression or activity of glutamate dehydrogenase (NtGDH), wherein the NtGDH comprises, consists, or consists essentially of at least one of a NtGDH2 polynucleotide or a NtGDH3 polynucleotide or a NtGDH6 polynucleotide or a NtGDH9 polynucleotide or a NtGDH10 polynucleotide or a NtGDH12 polynucleotide or a NtGDH2 polypeptide or a NtGDH3 polypeptide or a NtGDH6 polypeptide or a NtGDH9 polypeptide or a NtGDH10 polypeptide or a NtGDH12 polypeptide, wherein the expression of the NtGDH2 polynucleotide or the NtGDH3 polynucleotide or the NtGDH6 polynucleotide or the NtGDH9 polynucleotide or the NtGDH10 polynucleotide or the NtGDH12 polynucleotide or the NtGDH2 polypeptide or the NtGDH3 polypeptide or the NtGDH6 polypeptide or the NtGDH9 polypeptide or the NtGDH10 polypeptide or the NtGDH12 polypeptide is modulated as compared to a control plant in which the expression of the NtGDH2 polynucleotide or the NtGDH3 polynucleotide or the NtGDH6 polynucleotide or the NtGDH9 polynucleotide or the NtGDH10 polynucleotide or the NtGDH12 polynucleotide or the NtGDH2 polypeptide or the NtGDH3 polypeptide or the NtGDH6 polypeptide or the NtGDH9 polypeptide or the NtGDH10 polypeptide or the NtGDH12 polypeptide is not modulated.

The invention belongs to the technical field of biology, and discloses a plasmid system with high rAAV yield and application. The plasmid system of the high-yield rAAV comprises a transgenic plasmid containing two terminal inverted repetitive sequences, a packaging plasmid, an auxiliary plasmid and an shRNA (short hairpin Ribonucleic Acid) expression cassette, the shRNA expression cassette comprises a sequence capable of targeting a target gene in the transgenic plasmid. According to the plasmid system, in the rAAV production process, transcription expression of a target gene is inhibited by expressing a target gene coding region or a target sequence on shRNA targeted transgenic plasmids. The well-designed target sequence is different from human and mammal source gene coding region sequences, and can be applied to various transgenic plasmids in a broad spectrum manner, so that the yield of the rAAV is increased, the rAAV production efficiency of mammal cells can be remarkably improved, and the technical effects of improving the druggability of the rAAV and reducing the production cost are achieved.

The invention relates to a genetically modified licorice stem cell and a preparation method thereof, and relates to the technical field of genetic engineering, and the licorice stem cell is modified by introducing a gene coding dimethyl allyl transferase (PcM4DT) or an active fragment thereof and a gene coding isoflavone synthase (IFS) or an active fragment thereof into the licorice stem cell. The content of secondary metabolites in the genetically modified liquorice stem cells is remarkably increased compared with that of unmodified liquorice stem cells, the increased secondary metabolites meet the industrial production requirement and can be directly used for high-end skin care products and medical products, and the liquorice resource value is expanded.

The invention provides an application of a CP gene, or a protein encoded by the CP gene, or a recombinant vector or host bacteria containing a CP gene coding region in down-regulation of the MeGRXC3 expression level. Researches find that CP and MeGRXC3 interact with each other, so that the growth ability of yeast in an SD / -Leu / -Trp / -His / -Ade auxotrophic culture medium can be improved, cell autophagy can be activated, the expression of MeGRXC3 can be down-regulated, and the active oxygen content of plant tissues co-expressed with MeGRXC3 can be increased. Research results lay a theoretical foundation for breeding of common cassava mosaic virus disease resistance.

The invention relates to the field of genetic engineering, in particular to an optimized bacillus subtilis expression vector, a genetically engineered bacterium containing the expression vector and application of the genetically engineered bacterium. The vector is based on pMA5 plasmids, and a shuttle type bacillus subtilis expression vector is optimized; an open reading framework with unknown functions in the bacillus subtilis expression vector is reduced, so that the size of the vector is reduced, the expression burden of cells is reduced, and an optimization effect is achieved; the optimized bacillus subtilis expression vector not only can be stably copied in escherichia coli, but also can be stably expressed in bacillus subtilis, and the expression level of heterologous gene coding protein is improved by utilizing the genetically engineered bacteria containing the optimized bacillus subtilis expression vector.

The invention discloses application of Dendrobium huoshanense DhOMT8 gene coded methyltransferase in biosynthesis of a flavonoid compound for regulating gastrointestinal activity, and belongs to the technical field of biology. The nucleotide sequence of the DhOMT8 gene encodes a nucleotide sequence of an amino acid sequence as shown in SEQ ID NO. 2. The methylated flavonoid compound is obtained by a biological synthesis method for carrying out enzyme catalysis by using the DhOMT8 protein in vitro, and a new method is provided for synthesizing the flavonoid compound for regulating gastrointestinal activity.

The invention discloses a stem cell-derived function-enhanced mesenchymal stromal cell as well as a preparation method and application thereof. The preparation method comprises the following steps: inserting an IL-10 gene coding sequence and a CD47 gene coding sequence into a human pluripotent stem cell; a neural crest cell differentiation culture medium containing a Wnt pathway agonist and an ALK5 inhibitor is used for carrying out induced differentiation culture on the CD47 positive human pluripotent stem cells to obtain the neural crest cells. The neural crest cells are subjected to induced differentiation culture by adopting a transition culture medium containing an epidermal growth factor, a fibroblast growth factor 2 and a cell apoptosis inhibitor and a mesenchymal stromal cell differentiation culture medium containing the epidermal growth factor, the fibroblast growth factor 2 and a platelet growth factor in sequence; according to the present invention, with the combination of the specific embodiments, the obtained mesenchymal stromal cell highly expresses CD47 and IL-10, the expression of the CD47 can help the transplanted cell escape from the attack of the immune system and prolong the cell survival time, and the expression of the IL-10 can be used for anti-inflammation.

The present invention relates to the field of genetic engineering technology, and specifically discloses a protein AaBBX21 for increasing the anthocyanin content of plants and the application of its encoding gene. The protein AaBBX21, whose gene encoding sequence is shown in SEQ ID No.1; after the encoding gene of AaBBX21 is overexpressed in plants by genetic engineering means, it is used to increase the anthocyanin content, ornamental value and edible value of plants. The present invention adopts genetic engineering technology to clone the AaBBX21 transcription factor from the succulent plant 'Halloween', and performs bioinformatics analysis, plant overexpression vector construction, Agrobacterium-mediated genetic transformation and phenotypic analysis of transgenic Arabidopsis thaliana on AaBBX21. The AaBBX21 gene identified by the present invention can significantly increase the anthocyanin content in plant tissues and the ornamental value and edible value of plants. The present invention lays a solid foundation for the subsequent use of genetic engineering technology to carry out transgenic precision breeding to improve the ornamental value and edible value of plants, and the AaBBX21 gene has great application prospects and market potential.

Provided herein are kits, compositions, systems, and methods for treating a subject with a tumor with kynurenine responsive bacteria (e.g., such that the bacteria reduce or eliminate said tumor). In certain embodiments, the kynurenine responsive bacteria comprise one or more nucleic acid sequences comprising: i) a first nucleotide sequence (e.g., not endogenous to said bacteria) encoding a kynurenine transcriptional regulator protein, and ii) a second nucleotide sequence (e.g., not endogenous to said bacteria or overexpressing an endogenous equivalent protein) encoding a kynurenine transporter protein. In particular embodiments, the kynurenine responsive bacteria further comprise at least one of the following: i) a third nucleotide sequence comprising a kynurenine responsive promoter upstream of a plasmid replication gene encoding a plasmid replication protein, ii) a fourth nucleotide sequence comprising a kynurenine responsive promoter upstream of a reporter gene encoding a reporter protein, and iii) a fifth or more nucleotide sequence comprising a kynurenine responsive promoter upstream of a gene essential for bacterial growth, or a gene codes a for therapeutic payload.

The invention discloses a method for improving citrus liberobacter asiaticum resistance by using a CsZRK1 gene, the CsZRK1 gene is mutated to improve the liberobacter asiaticum resistance of citrus plants, and the nucleotide sequence of the CsZRK1 gene is as shown in SEQ ID NO: 1. The CsZRK1 gene coding sequence is cloned, the CRISPR / Cas9 mutant vector is constructed, and then the citrus is converted, so that the incidence degree of the hairy root huanglongbing of the obtained transgenic plant is obviously reduced, and the CsZRK1 gene has a great application value for huanglongbing-resistant breeding of the citrus, and can be used as a candidate gene for huanglongbing-resistant breeding with a plurality of huanglongbing-resistant and susceptible genes.

The invention discloses application of SNP sites in identifying or screening watermelon fruit shapes, and belongs to the technical field of molecular biology. The SNP site is located at the 727th site of a Cla97C03G066390 gene coding region on a chromosome 3 of a watermelon Watermelon (97103) v2 genome, and the SNP site has G / A polymorphism and is applied to identification or screening of watermelon fruit shapes. The KASP molecular marker primer group for detecting the SNP site comprises specific reverse primers as shown in SEQ ID NO.2 and SEQ ID NO.3, and a universal forward primer as shown in SEQ ID NO.4. The invention also discloses a kit for detecting the KASP molecular marker primer group for detecting the SNP site. The SNP locus and the developed KASP molecular marker primer group can quickly perform genetic typing of watermelon fruit shapes, are accurate and efficient in detection and convenient and stable in amplification, and can be used for molecular marker-assisted selection and watermelon germplasm resource improvement.

A genetically modified yeast cell, wherein the yeast cell comprises a genetic modification comprising overexpression of yeast gene encoding porphobilinogen deaminase (HEM3), the HEM3 gene having at least 80% identity with SEQ ID No. 7. The genome of the modified yeast cell further comprises one or more genetic modifications in one or more genes selected from: genes coding for heme-dependent repressor of hypoxic genes (ROX1), genes coding for heme oxygenase (HMX1), genes coding for a receptor for vacuolar proteases (VPS10), and genes coding for vacuolar proteinase (PEP4), the one or more genetic modifications being such that expression of a polypeptide from such a gene is reduced or disrupted or the polypeptide expressed is non-functional.

The invention relates to the technical field of plant genetic engineering, in particular to application of an algae NySAT2 gene in regulation and control of alfalfa crude protein and detergent fiber. According to the invention, a sequence after codon optimization of an NySAT2 gene coding region is obtained by using gene synthesis and codon optimization technologies, an algae NySAT2 gene overexpression vector is constructed, a target gene is introduced into an alfalfa genome by using an agrobacterium rhizogenes mediated genetic transformation method, and a transgenic plant is obtained by using a genetic transformation system. Molecular identification and phenotypic analysis show that on the premise of keeping normal growth vigor (plant height and biomass have no significant difference from those of a wild type), the crude protein content of a medicago sativa plant with the overexpressed NySAT2 gene is remarkably increased compared with that of the wild type medicago sativa plant, and meanwhile, the contents of acid detergent fibers and neutral detergent fibers are remarkably reduced, so that the content of the crude protein in the medicago sativa plant with the overexpressed NySAT2 gene is remarkably increased. The forage quality and the feeding value of the medicago sativa are effectively improved.

The invention discloses a modified aureobasidium pullulans strain based on reduction of malic acid pathway consumption and application of the modified aureobasidium pullulans strain. Gene for coding NADPH dependent malic enzyme and phosphoenolpyruvate carboxykinase in an aureobasidium pullulans genome is directionally knocked out. The transformation blocks a key consumption branch for synthesis and accumulation of the cytoplasmic malic acid, and effectively guides a central carbon metabolic flow to be more efficiently guided to a target product. Experimental results show that the polymalic acid yield and the saccharic acid conversion rate of the double-knockout engineering strain D3N5-delta3617 / deltapepck in fermentation are remarkably improved compared with those of an original strain, and the biomass is also increased. The invention provides a high-performance strain with important application value for industrial efficient fermentation production of polymalic acid.