159 results about "Gene code" patented technology

The invention relates to a diacylglycerol acyltransferase capable of synthesizing triglyceride as well as a coding gene and application of the diacylglycerol acyltransferase, and belongs to the field of enzymology, the nucleotide sequence of the gene for coding the diacylglycerol acyltransferase is as shown in SEQ ID NO.1, and the amino acid sequence of the diacylglycerol acyltransferase is as shown in SEQ ID NO.2. The invention also provides a recombinant plasmid and a recombinant engineering bacterium containing the gene of the enzyme. Diglyceride and an acyl donor are used as substrates, and triglyceride is catalytically synthesized through an acyl transfer reaction by using the enzyme. The invention provides an efficient tool for realizing enzymatic biosynthesis and large-scale production of natural triglyceride.

The invention relates to the technical field of plant genetic engineering, in particular to application of an algae NySAT2 gene in regulation and control of alfalfa crude protein and detergent fiber. According to the invention, a sequence after codon optimization of an NySAT2 gene coding region is obtained by using gene synthesis and codon optimization technologies, an algae NySAT2 gene overexpression vector is constructed, a target gene is introduced into an alfalfa genome by using an agrobacterium rhizogenes mediated genetic transformation method, and a transgenic plant is obtained by using a genetic transformation system. Molecular identification and phenotypic analysis show that on the premise of keeping normal growth vigor (plant height and biomass have no significant difference from those of a wild type), the crude protein content of a medicago sativa plant with the overexpressed NySAT2 gene is remarkably increased compared with that of the wild type medicago sativa plant, and meanwhile, the contents of acid detergent fibers and neutral detergent fibers are remarkably reduced, so that the content of the crude protein in the medicago sativa plant with the overexpressed NySAT2 gene is remarkably increased. The forage quality and the feeding value of the medicago sativa are effectively improved.

The invention discloses a modified aureobasidium pullulans strain based on reduction of malic acid pathway consumption and application of the modified aureobasidium pullulans strain. Gene for coding NADPH dependent malic enzyme and phosphoenolpyruvate carboxykinase in an aureobasidium pullulans genome is directionally knocked out. The transformation blocks a key consumption branch for synthesis and accumulation of the cytoplasmic malic acid, and effectively guides a central carbon metabolic flow to be more efficiently guided to a target product. Experimental results show that the polymalic acid yield and the saccharic acid conversion rate of the double-knockout engineering strain D3N5-delta3617 / deltapepck in fermentation are remarkably improved compared with those of an original strain, and the biomass is also increased. The invention provides a high-performance strain with important application value for industrial efficient fermentation production of polymalic acid.

The application discloses a biosynthesis method of blue copper peptide, comprising the following steps: synthesizing a recombinant protein according to an amino acid sequence shown in SEQ ID NO. 1, synthesizing a gene coding the recombinant protein, constructing a recombinant plasmid by using the gene, transferring the recombinant plasmid into a host bacterium, carrying out fermentation culture to induce protein expression, separating the protein, carrying out enzyme cutting and purification, and finally carrying out complexing with copper ions to obtain the blue copper peptide. Compared with the prior art, the biosynthesis method of the blue copper peptide has the advantages of low production cost, green environmental protection and high yield by optimizing the design of the fusion protein, and the yield of the final product can be significantly improved, and the fusion protein has better structure and isoelectric point characteristics.

The invention provides application of an FZD4 gene. The FZD4 gene is used for regulating osteogenic differentiation and mineralization of chicken bone marrow mesenchymal stem cells. The FZD4 gene in the chicken bone marrow mesenchymal stem cells is over-expressed and is used for promoting osteogenic differentiation and mineralization of the chicken bone marrow mesenchymal stem cells; or / and the FZD4 gene in the chicken bone marrow mesenchymal stem cells is knocked down to inhibit osteogenic differentiation and mineralization of the chicken bone marrow mesenchymal stem cells; the nucleotide sequence of the coding region of the FZD4 gene is as shown in SEQ ID NO. 1. According to the invention, the positive regulation effect of the FZD4 gene in osteogenic differentiation of chicken bone marrow mesenchymal stem cells is determined for the first time, the blank of a bone regulation mechanism of the poultry FZD4 gene is filled, and a new biological preparation target is provided for prevention and treatment of poultry bone diseases; the FZD4 gene can also be used as a molecular marker for breeding chicken varieties with high bone strength.

The invention relates to the field of biology, and particularly provides a replication-deficient influenza virus vaccine skeleton which comprises a PA gene, an HA gene, an NP gene, an NA gene, an M gene, an NS gene and a PB2 gene of an influenza virus. The PB2 gene is replaced into a PB1 gene coding region of a vaccine skeleton; the open reading frame of the original PB2 gene can be replaced by an exogenous gene, so that the exogenous gene is inserted into the vaccine skeleton and expressed. The skeleton has the advantages that the capacity is large, the antigen can be efficiently and stably expressed, the passage is stable, and the like. Meanwhile, the invention also discloses a replication-defective influenza virus as well as a preparation method and application thereof.

The invention discloses wheat TaPAB1 protein and application of related biological materials of the wheat TaPAB1 protein in the aspect of improving the grain weight of wheat. A gene for coding the TaPAB1 protein is named as a TaPAB1 gene. According to the invention, the gene TaPAB1 is cloned from a wheat cultivation variety, Chinese spring, and is over-expressed in a wheat variety Kenong 199 (KN199) by utilizing a gene engineering technology, so that the thousand seed weight, the grain length, the plant height, the ear length and the yield of a TaPAB1 over-expressed plant are obviously increased compared with those of a wild type. The invention provides a research basis for increasing the wheat yield and breeding high-yield varieties.

The invention relates to euphausia superba-derived diacylglycerol acyltransferase as well as a coding gene and application thereof, and belongs to the field of enzymology, the nucleotide sequence of the gene for coding the enzyme is as shown in SEQ ID NO.1, and the amino acid sequence of the enzyme is as shown in SEQ ID NO.2. The invention further discloses a preparation method of the euphausia superba-derived diacylglycerol acyltransferase. The invention also provides a recombinant plasmid and a recombinant engineering bacterium containing the gene of the enzyme. Diglyceride and an acyl donor are used as substrates, and triglyceride is catalytically synthesized through an acyl transfer reaction by using the enzyme. The invention provides an efficient tool for realizing enzymatic biosynthesis and large-scale production of natural triglyceride.

The invention discloses an overexpression vector of LAMP2B and application thereof, which can effectively delay the disease progress of DMD, and comprises: (a) an AAV vector which is an adeno-associated virus vector and has high muscle tissue targeting and low liver tropism; (b) a CMV (cytomegalovirus) promoter, wherein the CMV promoter is a cytomegalovirus promoter; (c) a coding sequence of an hLAMP2B gene, namely a coding sequence of a human LAMP2B gene, and the coding sequence of the hLAMP2B gene is operably linked to the CMV promoter; wherein the vector is configured to overexpress LAMP2B protein in muscular tissue, and the vector is self-complementary AAV, namely scAAV, the concept is ingenious, the novel AAV vector with high muscle transformation efficiency is utilized to deliver a complete LAMP2B gene (LAMP2B is a gene related to autophagy regulation) into muscle cells of all organs of a mouse (AAV-hLAMP2B for short), and the autophagy regulation function of the mouse is improved. The muscle tissue function of the mouse DMD disease model is comprehensively assisted to recover, and the disease progress of the DMD is delayed.

The application discloses application of a rice metal tolerance protein coding gene OsMTP7 The rice metal tolerance protein coding gene OsMTP7 has the accession number of AK241225.1 in Genbank. The gene coding protein has zinc transport activity, can increase the zinc content of rice root system and above-ground part, and significantly increases the accumulation of zinc in rice grains.

The invention belongs to the technical field of drilling fluid deironing equipment, and provides a drilling fluid demagnetizing device parameter optimization method based on a genetic algorithm and a related device.The method comprises the steps that structure and process parameters of a drilling fluid demagnetizing device are obtained and converted into individual codes of the genetic algorithm, design parameters of the drilling fluid demagnetizing device are obtained and serve as gene codes, and the genetic algorithm is used as the individual codes of the drilling fluid demagnetizing device; each individual represents a device configuration parameter; constructing a fitness function based on the adsorption efficiency, the processing capacity and the cost of the drilling fluid demagnetizing device; and based on the fitness function, evaluating the fitness of each individual, performing selection, crossover and mutation operation on the population to generate a new generation of individuals, and when a predetermined evolution algebra is reached or the fitness reaches a predetermined threshold, completing optimization. Various parameters are allowed to be adjusted according to actual conditions by adopting a parameter optimization algorithm so as to adapt to different types of fluids containing magnetic substances, the flexibility is high, and the application field is wider.

This invention discloses an encoding method and system based on the projection constant C = 0.0017 and the digital gene 428571. The method determines the projection dimension parameters using the four-constant formula C = (2^N - 2^(N-2)) / 7 × 10^ exponent, introduces the digital gene sequence 428571 as the information encoding benchmark, achieves dimension mapping through binary-to-octal conversion, and establishes an 18-bit octal identity gene code generation model. This invention can be applied to proactive digital identity authentication systems, improving computational accuracy and information security through projection constant correction.

The invention belongs to the technical fields of immunotherapy, bioengineering, gene therapy and cell therapy, and provides a preparation method of an enhanced CAR-T cell for overexpressing LYAR, and the enhanced CAR-T cell and application thereof in order to solve the problems that the killing function of the CAR-T cell in solid tumor treatment is limited, the durability is poor, and function depletion is likely to occur. CDNA of the LYAR gene is cloned to an overexpression plasmid vector and packaged into lentivirus, T cells are jointly infected by the lentivirus and the CAR expression virus, the LYAR gene is forced to express under the driving of an EF-1alpha promoter, and stable overexpression of the LYAR in the CAR-T cells is achieved; and the CAR is a CAR targeting EGFR (epidermal growth factor receptor) and CD19. According to the present invention, the gene coding LYAR and the CAR gene are simultaneously transferred into the T cell through the lentivirus infection technology, and are compulsively expressed under the action of the EF1 alpha promoter, such that the anti-tumor function of the CAR-T cell can be significantly enhanced, and the activation and killing functions of the immune cell can be improved;

The invention belongs to the technical field of tea tree gene application, and particularly relates to application of a tea tree CsPHT gene in improving tea geometrid and colletotrichum gloeosporioides resistance of a tea tree. The invention discloses a tea tree CsPHT gene, the nucleotide sequence of the tea tree CsPHT gene is as shown in SEQ ID NO.1, the tea tree CsPHT gene codes 434 amino acid residues, and the amino acid sequence of the tea tree CsPHT gene is as shown in SEQ ID NO.2. The CsPHT gene is proved to be capable of catalyzing biosynthesis of tea tree defense related compounds such as feruloyl putrescine, coumaroyl putrescine and cinnamoyl putrescine for the first time, and the conservation of the catalytic function of the CsPHT gene is proved through heterologous expression. An in-vivo instantaneous function verification result of a tea tree shows that the over-expression of the CsPHT gene can significantly improve the resistance of the tea tree to tea geometrid and tea anthracnose bacteria. The CsPHT gene is proved to be a key positive regulation factor for regulating the resistance of the tea tree to the tea geometrid and the tea anthracnose fungus. The invention provides important gene resources and molecular targets for cultivating new varieties of high-resistance and multi-resistance tea trees.

The invention belongs to the technical field of biology, and particularly relates to Dendrobium officinale DoORP1 protein and a coding gene and application thereof. The invention discloses a Dendrobium officinale DoORP1 protein, which comprises a protein composed of an amino acid sequence as shown in SEQ ID NO.6, and a protein which is subjected to substitution and / or deletion and / or addition of one or more amino acid residues, is related to plant drought tolerance and is derived from the Dendrobium officinale DoORP1 protein. The invention finds that overexpression of the gene coding the DoORP1 protein enables an overexpression strain to show stronger tolerance under salt stress of a plant, and the overexpression strain specifically shows that the root is longer in a seedling stage and the chlorophyll content is higher in a growth stage. Under drought stress, the overexpression strain also shows stronger tolerance, and compared with a wild type, the overexpression strain has the advantages that the root length in the seedling stage is increased, and the survival rate in the growth stage is higher.

The application provides a KRT31 gene knockout cell line and its application in promoting replication of viruses of the Picornaviridae family and / or production of vaccines of viruses of the Picornaviridae family, and belongs to the technical field of genetic engineering. The application provides an application of a KRT31 gene or a coded protein thereof as a target in preparation of a product for regulating replication of viruses of the Picornaviridae family and / or vaccine production. Up-regulation of the expression level of the KRT31 gene can inhibit replication of viruses of the Picornaviridae family, and down-regulation of the expression level of the KRT31 gene can promote replication of viruses of the Picornaviridae family. The application adopts sgRNA to knockout the KRT31 gene to prepare a cell line with lost function of a gene coded protein, so as to promote replication of viruses of the Picornaviridae family, improve virus titer and antigen yield, and help to efficiently prepare vaccines of viruses of the Picornaviridae family.

According to the application of the kiwi fruit transcription factor AcbZIP87 gene in gray mold resistance, after the expression of the gene is inhibited, the resistance of kiwi fruit to gray mold can be remarkably reduced. A gene coding series of the cloned AcbZIP87 gene is shown as SEQ ID NO. 1, and the effect of the AcbZIP87 gene in resisting gray mold in kiwi fruits is proved, specifically, compared with a control group after virus inoculation, the disease symptoms of obtained transgenic fruits are aggravated, the infected area is obviously enlarged, the enzyme activity of SOD, POD and CAT in the fruits is also obviously reduced, and the effect of resisting gray mold in kiwi fruits is proved. And important gene data support is provided for disease-resistant breeding of kiwi fruits.

The invention relates to the field of genetic engineering, in particular to a non-methanol-induced chitin deacetylase pichia pastoris expression vector and engineering bacteria as well as a construction method and application of the non-methanol-induced chitin deacetylase pichia pastoris expression vector and engineering bacteria. According to the invention, a carrier containing a constitutive strong promoter is adopted as a skeleton, a chitin deacetylase gene coding sequence is inserted into the carrier, methanol is not required to be used as an inducer when the obtained recombinant plasmid is applied, and the produced chitin deacetylase is efficient, green and environment-friendly, and can be applied to the fields of beauty industry, agriculture, biomedicine and the like. The invention establishes a non-methanol-induced chitin deacetylase pichia pastoris expression vector and engineering bacteria, which are used for high-efficiency expression of chitin deacetylase, and solves the problem of low enzyme yield of chitin deacetylase from natural sources.

This invention discloses the application of the OsCAD3 protein and its encoding gene in regulating resistance to bacterial blight in rice, relating to the field of plant genetic engineering. The amino acid sequence of this gene protein is shown in SEQ ID NO.3. Cloning the gene editing site sequence, constructing a gene editing recombinant vector, and transforming it into rice can improve rice resistance to bacterial blight and ensure stable rice yield. The OsCAD3 gene editing vector constructed in this invention and the new OsCAD3 allele formed through genome editing can be applied to rice breeding for resistance to bacterial blight. Constructing an overexpression recombinant vector of the above gene coding region sequence and transforming it into rice can reduce rice resistance to bacterial blight and ensure stable rice yield. The above gene overexpression vector can be used in rice breeding to regulate bacterial blight. This invention can be used in the fields of rice germplasm resource improvement and genetic breeding, and is of great significance for breeding new rice varieties resistant to bacterial blight.

The invention belongs to the technical field of genetic engineering, and particularly relates to application of diaphorina citri transferrin Tsf in prevention and control of citrus huanglongbing. The invention provides an application of diaphorina citri transferrin Tsf in prevention and control of citrus huanglongbing. A nucleotide sequence for coding a gene of the diaphorina citri transferrin Tsf is shown as SEQ ID NO.1. The invention further provides a preparation method of the diaphorina citri transferrin Tsf. According to the invention, the expression of transferrin Tsf in diaphorina citri can be interfered, so that the infection ability of Candidatus Liberobacter asiaticum can be reduced, the bacterium carrying amount can be reduced, a new strategy is provided for effective prevention and control of the Candidatus Liberobacter asiaticum, and a new prevention and control idea is provided for controlling propagation of bacterial pathogens by means of vector insects.

The application discloses a tobacco NtGLV11 gene, a protein, and polypeptide coding, and application and preparation and a preparation method of the polypeptide in promoting plant yellowing. The tobacco NtGLV11 gene codes the NtGLV11 protein, and the NtGLV11 polypeptide is generated after the NtGLV11 protein is cut and chemically modified. It is found for the first time that the NtGLV11 polypeptide is involved in tobacco yellowing regulation, and no report is found in crops including tobacco at present. The NtGLV11 polypeptide hormone of the application promotes tobacco senescence and yellowing, and has simple preparation and use methods, strong operability, effectively promotes tobacco senescence and yellowing, and has biological safety.

The present invention relates to an improved cysteine-producing strain, in particular to a microbial strain suitable for the fermentative production of L-cysteine, characterized in that the relative enzymatic activity of the enzymes identified by the number EC 2.7. 9.2 in the KEGG database is inactivated or the specific activity is reduced based on the wild-type enzymes, and the enzyme represented by the number EC 2.7. 9.2 in the KEGG database forms an increased amount of L-cysteine as compared to a microbial strain having a wild-type enzymatic activity in which the gene encoding the enzymatic activity is represented by ppsA. The invention also provides methods of producing L-cysteine using these microbial cells.

The invention relates to the technical field of plant molecular breeding and molecular biology, in particular to a KASP molecular marker related to soybean mosaic virus resistance and application of the KASP molecular marker. The molecular marker is targeted to an anti-soybean mosaic virus specific functional SNP (Single Nucleotide Polymorphism) site of a soybean GmLMM4 gene; the SNP locus is c.743Cgt of a soybean GmLMM4 gene coding region, and the SNP locus is a nucleotide sequence of the soybean GmLMM4 gene; the GmLMM4 gene is mutated by a T mononucleotide, and the 248th amino acid of the GmLMM4 gene coding protein is changed into isoleucine from threonine through the mutation. The molecular marking tool provided by the invention has the advantages that the accuracy of anti-SMV breeding can be remarkably improved, the matched primer group is designed in a targeted antisense strand, and the C / C, C / T and T / T genotypes can be accurately distinguished by FAM / HEX fluorescence signals and correspond to soybean anti-SMV phenotypes; high-throughput detection is completed in the seedling stage, the screening efficiency of high-resistance materials is improved, the identification period is shortened, and the screening efficiency of soybean anti-SMV breeding is improved.

The invention belongs to the field of biotechnology and plant breeding, and discloses a method for creating haploids by using rice endogenous genes. The invention relates to an application of an OsKPL1 gene or an OsKPL1 gene and an OsKPL2 gene in creating a rice haploid induction system or creating a rice haploid, and the gene number of the OsKPL1 gene is LOCOs04g51260, and the gene number of the OsKPL2 gene is LOCOs04g51290. Researches show that a single mutant of the oskpl1 has haploid inducibility of about 0.5%, double mutants of the oskpl1 and the oskpl2 have haploid inducibility of about 1.6%, and additional gene resources are provided for haploid breeding in rice.

The invention discloses application of a corn Zm00001d028750 gene in regulation and control of grain traits, and relates to the technical field of bioengineering, a nucleotide sequence of a coding region of the Zm00001d028750 gene is as shown in SEQ ID NO.1, and an amino acid sequence of a protein coded by the Zm00001d028750 gene is as shown in SEQ ID NO.2. The invention further discloses application of the corn Zm00001d028750 gene in regulation and control of grain traits and application of the corn Zm00001d028750 gene in regulation and control of grain traits. According to the application of the corn Zm00001d028750 gene in regulation and control of grain traits, the hundred-grain weight of corn is an important factor influencing the yield of corn, and along with the increase of the hundred-grain weight of corn, the higher the seed yield is, the higher the yield is; lysine is an essential amino acid for human beings and monogastric animals, and the eating and feeding values of corn can be remarkably improved by increasing the content of lysine and total amino acids.

The invention relates to the technical field of biological genes, in particular to a gene directed editing system applied to mycoplasma bovis, which is an editing system for expressing a combination of a single plasmid of GP35 single-chain annealing protein and a targeting fragment ssDNA (single-stranded deoxyribonucleic acid), and comprises an expression plasmid of a GP35 gene and the ssDNA of the targeting fragment. The invention relates to a gene directed editing method applied to mycoplasma bovis. The gene directed editing method comprises the following steps: constructing a mycoplasma bovis strain for expressing GP35 single-chain annealed protein, constructing an ssDNA single chain of a targeting fragment, and performing gene editing on a target gene to obtain a pdhC gene deleted strain. According to the invention, a KanR gene is successfully inserted at the 158bp position of a pdhC gene coding region of a mycoplasma bovis PG45 strain, so that a mycoplasma bovis pdhC gene oriented deletion strain is obtained. According to the invention, the metabolism-related gene directed deletion strain M. bovis PG45 [delta] pdhC is successfully constructed, and the pdhC protein is characterized to have the ability of regulating the growth rate of mycoplasma bovis and maintaining colonial morphology.

The application discloses a lipase mutant LipM1718, the amino acid sequence of the lipase mutant LipM1718 is shown as SEQ ID NO. 2, and the lipase mutant LipM1718 comprises eleven mutation sites of E61D, Q72N, N73A, P86A, N116A, A117P, I126V, Y134F, Q194R, A260I and S277T; the nucleotide sequence of a gene coding the lipase mutant LipM1718 is shown as SEQ ID NO. 1. The application constructs a recombinant carrier pET22b- LipM1718 containing the lipase mutant LipM1718, which is expressed in a prokaryotic host cell Escherichia coli BL21 (DE3). Experiment proves that the obtained lipase has high efficient catalytic activity of hydrolysis of ester substrates after the gene is expressed in the prokaryotic host cell, and has great application potential in the harmless treatment and resource utilization field of kitchen waste oil.

The application discloses a tobacco NtGLV05 gene, a protein, and polypeptide coding, and application and preparation and a preparation method of the polypeptide in promoting plant yellowing. The tobacco NtGLV05 gene codes the NtGLV05 protein, and the NtGLV05 polypeptide is generated after the NtGLV05 protein is cut and chemically modified. It is found for the first time that the NtGLV05 polypeptide is involved in tobacco yellowing regulation, and no report is found in crops including tobacco at present. The NtGLV05 polypeptide hormone of the application promotes tobacco senescence and yellowing, and has simple preparation and use methods, strong operability, effectively promotes tobacco senescence and yellowing, and has biological safety.

An ornithine decarboxylase gene related to Auricularia auricula-judae degradation and application thereof belong to the field of bioengineering technology. In order to solve the technical problem that there is lack of key functional genes and molecular targets which can be used for early identification, anti-degradation molecular breeding and strain rejuvenation due to continuous subculture leading to degradation in Auricularia auricula-judae industry, based on the G1, G10 and G20 mycelium transcriptome difference analysis of Auricularia auricula-judae strain Heiwei S1908, the ornithine decarboxylase gene g4162 significantly differentially expressed between normal mycelium and G20 degraded mycelium is screened, the molecular function of the gene in the degradation of Auricularia auricula-judae subculture is determined, and the system biology information prediction of the gene coding protein is completed, which provides core gene resources and theoretical basis for the degradation mechanism research, molecular detection kit development, anti-degradation germplasm screening and gene breeding of Auricularia auricula-judae.

The invention relates to the technical field of plant breeding, in particular to application of MYC transcription factor related biological products to improvement of low potassium resistance of upland cotton. The MYC type transcription factor comprises GhMYC2bA or / and GhMYC2dA, and the MYC type transcription factor comprises GhMYC2bA or / and The base sequence of the GhMYC2bA gene for coding the GhMYC2bA type transcription factor is as shown in SEQ ID NO. 1 (sequence identifier number 1). The GhMYC2bA or GhMYC2dA gene in the cotton is overexpressed through a gene engineering method, the adaptive capacity of the cotton in a low-potassium environment can be remarkably enhanced, and the expression is that the plant growth is improved, the dry matter accumulation is increased, the potassium ion absorption efficiency is improved, and the leaf yellowing symptom is relieved. The invention provides a new gene target and a new breeding strategy for cultivating a low-potassium-tolerant cotton variety, and has important significance for solving the problem of potassium deficiency in farmland and ensuring stable and high yield of cotton.