255 results about "Gene code" patented technology

The invention discloses application of wheat Rht1-D1b protein in regulation and control of a wheat tillering angle, and belongs to the technical field of biological breeding. The technical problem to be solved by the invention is how to increase the tillering angle of plants. Therefore, the invention provides the application of the protein or a substance for regulating and controlling the expression of a protein coding gene or a substance for regulating and controlling the activity or content of the protein in regulating and controlling the tillering angle of the plant, wherein the protein can be Rht1-D1b protein; the Rht1-D1b protein can be a protein of which the amino acid sequence is as shown in SEQ ID No. 3. According to the application, the Rht1-D1b gene is finally localized through gene localization and map-based cloning. The gene significantly increases the tillering angle of wheat. The wheat gene Rht1-D1b can be widely applied to the plant fields of wheat germplasm resource improvement, wheat plant type genetic breeding and the like, and has an important application value for improving the wheat yield.

The invention discloses a corn ZmMYBR37 gene and an application of the corn ZmMYBR37 gene in regulating and controlling the size of corn kernels. The nucleotide sequence of the ZmMYBR37 gene is as shown in SEQ ID No: 1 in a sequence table. A mutant material with small grains and reduced hundred-grain weight is separated from a corn EMS mutant library, and a mutation site occurs on a function unknown gene ZmMYBR37, so that protein translation is terminated in advance. The information analysis finds that the gene coding protein has a conservative MYB structural domain, and the gene coding protein is speculated to belong to an MYB transcription factor family. Gene expression analysis finds that the gene is high in constitutive expression in corn and high in expression level in leaves and embryos. The corn material overexpressed with the ZmMYBR37 has the advantages that the grain size is increased, and the hundred-grain weight is obviously increased, so that the ZmMYBR37 has the potential of increasing the corn yield.

The invention relates to a diacylglycerol acyltransferase capable of synthesizing triglyceride as well as a coding gene and application of the diacylglycerol acyltransferase, and belongs to the field of enzymology, the nucleotide sequence of the gene for coding the diacylglycerol acyltransferase is as shown in SEQ ID NO.1, and the amino acid sequence of the diacylglycerol acyltransferase is as shown in SEQ ID NO.2. The invention also provides a recombinant plasmid and a recombinant engineering bacterium containing the gene of the enzyme. Diglyceride and an acyl donor are used as substrates, and triglyceride is catalytically synthesized through an acyl transfer reaction by using the enzyme. The invention provides an efficient tool for realizing enzymatic biosynthesis and large-scale production of natural triglyceride.

The male fertility restoration plant of the Solanaceae family is deficient or suppressed in the function of at least one of the fertility restoring genes (a)-(o). The fertility restoring genes (a)-(o) are genes coding for a protein or the like containing an amino acid sequence represented by any one of SEQ ID NOs: 1-5.

The present disclosure provides a method for producing a polyisoprenoid, which makes it possible to synthesize in vitro a polyisoprenoid having an unprecedented structure, such as a 100% cis-polyisoprenoid or a polyisoprenoid containing an allylic diphosphate derivative as an initiating terminal. The present disclosure relates to a method for producing a polyisoprenoid in vitro, which employs a gene coding for a neryl diphosphate synthase and rubber particles bound to a protein encoded by the gene, or a method for producing a polyisoprenoid, which includes introducing into a plant a vector in which a gene coding for a neryl diphosphate synthase is linked to a promoter having a promoter activity that drives laticifer-specific gene expression to express a protein encoded by the gene specifically in laticifers.

The invention relates to a genetically modified licorice stem cell and a preparation method thereof, and relates to the technical field of genetic engineering, and the licorice stem cell is modified by introducing a gene coding dimethyl allyl transferase (PcM4DT) or an active fragment thereof and a gene coding isoflavone synthase (IFS) or an active fragment thereof into the licorice stem cell. The content of secondary metabolites in the genetically modified liquorice stem cells is remarkably increased compared with that of unmodified liquorice stem cells, the increased secondary metabolites meet the industrial production requirement and can be directly used for high-end skin care products and medical products, and the liquorice resource value is expanded.

The invention discloses application of SNP sites in identifying or screening watermelon fruit shapes, and belongs to the technical field of molecular biology. The SNP site is located at the 727th site of a Cla97C03G066390 gene coding region on a chromosome 3 of a watermelon Watermelon (97103) v2 genome, and the SNP site has G / A polymorphism and is applied to identification or screening of watermelon fruit shapes. The KASP molecular marker primer group for detecting the SNP site comprises specific reverse primers as shown in SEQ ID NO.2 and SEQ ID NO.3, and a universal forward primer as shown in SEQ ID NO.4. The invention also discloses a kit for detecting the KASP molecular marker primer group for detecting the SNP site. The SNP locus and the developed KASP molecular marker primer group can quickly perform genetic typing of watermelon fruit shapes, are accurate and efficient in detection and convenient and stable in amplification, and can be used for molecular marker-assisted selection and watermelon germplasm resource improvement.

A genetically modified yeast cell, wherein the yeast cell comprises a genetic modification comprising overexpression of yeast gene encoding porphobilinogen deaminase (HEM3), the HEM3 gene having at least 80% identity with SEQ ID No. 7. The genome of the modified yeast cell further comprises one or more genetic modifications in one or more genes selected from: genes coding for heme-dependent repressor of hypoxic genes (ROX1), genes coding for heme oxygenase (HMX1), genes coding for a receptor for vacuolar proteases (VPS10), and genes coding for vacuolar proteinase (PEP4), the one or more genetic modifications being such that expression of a polypeptide from such a gene is reduced or disrupted or the polypeptide expressed is non-functional.

The invention relates to the technical field of plant genetic engineering, in particular to application of an algae NySAT2 gene in regulation and control of alfalfa crude protein and detergent fiber. According to the invention, a sequence after codon optimization of an NySAT2 gene coding region is obtained by using gene synthesis and codon optimization technologies, an algae NySAT2 gene overexpression vector is constructed, a target gene is introduced into an alfalfa genome by using an agrobacterium rhizogenes mediated genetic transformation method, and a transgenic plant is obtained by using a genetic transformation system. Molecular identification and phenotypic analysis show that on the premise of keeping normal growth vigor (plant height and biomass have no significant difference from those of a wild type), the crude protein content of a medicago sativa plant with the overexpressed NySAT2 gene is remarkably increased compared with that of the wild type medicago sativa plant, and meanwhile, the contents of acid detergent fibers and neutral detergent fibers are remarkably reduced, so that the content of the crude protein in the medicago sativa plant with the overexpressed NySAT2 gene is remarkably increased. The forage quality and the feeding value of the medicago sativa are effectively improved.

The invention discloses a modified aureobasidium pullulans strain based on reduction of malic acid pathway consumption and application of the modified aureobasidium pullulans strain. Gene for coding NADPH dependent malic enzyme and phosphoenolpyruvate carboxykinase in an aureobasidium pullulans genome is directionally knocked out. The transformation blocks a key consumption branch for synthesis and accumulation of the cytoplasmic malic acid, and effectively guides a central carbon metabolic flow to be more efficiently guided to a target product. Experimental results show that the polymalic acid yield and the saccharic acid conversion rate of the double-knockout engineering strain D3N5-delta3617 / deltapepck in fermentation are remarkably improved compared with those of an original strain, and the biomass is also increased. The invention provides a high-performance strain with important application value for industrial efficient fermentation production of polymalic acid.

The application belongs to the field of intelligent medical treatment, and particularly relates to a method for constructing a virus single-gene host adaptability prediction model, equipment and a medium. The method comprises the following steps: obtaining a training set virus single-gene data set and a host label; encoding the single genes in the virus single-gene data set in sequence to obtain an encoding feature matrix set; the encoding is as follows: using a sliding window method to traverse a gene sequence, the frequency of 64 different codons in a window is used to form a window codon frequency vector, the window codon frequency vector is used to represent a central codon of the window, and each codon of the gene sequence is represented as a window codon frequency vector in sequence; inputting the encoding feature matrix set into a residual network model to obtain a prediction label, and iteratively training based on a comparison between the prediction label and the host label to obtain a single-gene host adaptability prediction model. The model capable of predicting gene adaptability is trained by using the representation of codons, and the analysis of gene codon adaptability can be realized.

The application discloses a biosynthesis method of blue copper peptide, comprising the following steps: synthesizing a recombinant protein according to an amino acid sequence shown in SEQ ID NO. 1, synthesizing a gene coding the recombinant protein, constructing a recombinant plasmid by using the gene, transferring the recombinant plasmid into a host bacterium, carrying out fermentation culture to induce protein expression, separating the protein, carrying out enzyme cutting and purification, and finally carrying out complexing with copper ions to obtain the blue copper peptide. Compared with the prior art, the biosynthesis method of the blue copper peptide has the advantages of low production cost, green environmental protection and high yield by optimizing the design of the fusion protein, and the yield of the final product can be significantly improved, and the fusion protein has better structure and isoelectric point characteristics.

The invention provides application of an FZD4 gene. The FZD4 gene is used for regulating osteogenic differentiation and mineralization of chicken bone marrow mesenchymal stem cells. The FZD4 gene in the chicken bone marrow mesenchymal stem cells is over-expressed and is used for promoting osteogenic differentiation and mineralization of the chicken bone marrow mesenchymal stem cells; or / and the FZD4 gene in the chicken bone marrow mesenchymal stem cells is knocked down to inhibit osteogenic differentiation and mineralization of the chicken bone marrow mesenchymal stem cells; the nucleotide sequence of the coding region of the FZD4 gene is as shown in SEQ ID NO. 1. According to the invention, the positive regulation effect of the FZD4 gene in osteogenic differentiation of chicken bone marrow mesenchymal stem cells is determined for the first time, the blank of a bone regulation mechanism of the poultry FZD4 gene is filled, and a new biological preparation target is provided for prevention and treatment of poultry bone diseases; the FZD4 gene can also be used as a molecular marker for breeding chicken varieties with high bone strength.

The invention relates to the field of biology, and particularly provides a replication-deficient influenza virus vaccine skeleton which comprises a PA gene, an HA gene, an NP gene, an NA gene, an M gene, an NS gene and a PB2 gene of an influenza virus. The PB2 gene is replaced into a PB1 gene coding region of a vaccine skeleton; the open reading frame of the original PB2 gene can be replaced by an exogenous gene, so that the exogenous gene is inserted into the vaccine skeleton and expressed. The skeleton has the advantages that the capacity is large, the antigen can be efficiently and stably expressed, the passage is stable, and the like. Meanwhile, the invention also discloses a replication-defective influenza virus as well as a preparation method and application thereof.

The invention discloses wheat TaPAB1 protein and application of related biological materials of the wheat TaPAB1 protein in the aspect of improving the grain weight of wheat. A gene for coding the TaPAB1 protein is named as a TaPAB1 gene. According to the invention, the gene TaPAB1 is cloned from a wheat cultivation variety, Chinese spring, and is over-expressed in a wheat variety Kenong 199 (KN199) by utilizing a gene engineering technology, so that the thousand seed weight, the grain length, the plant height, the ear length and the yield of a TaPAB1 over-expressed plant are obviously increased compared with those of a wild type. The invention provides a research basis for increasing the wheat yield and breeding high-yield varieties.

The invention relates to euphausia superba-derived diacylglycerol acyltransferase as well as a coding gene and application thereof, and belongs to the field of enzymology, the nucleotide sequence of the gene for coding the enzyme is as shown in SEQ ID NO.1, and the amino acid sequence of the enzyme is as shown in SEQ ID NO.2. The invention further discloses a preparation method of the euphausia superba-derived diacylglycerol acyltransferase. The invention also provides a recombinant plasmid and a recombinant engineering bacterium containing the gene of the enzyme. Diglyceride and an acyl donor are used as substrates, and triglyceride is catalytically synthesized through an acyl transfer reaction by using the enzyme. The invention provides an efficient tool for realizing enzymatic biosynthesis and large-scale production of natural triglyceride.

The invention provides a gene for regulating and controlling thermo-sensitive male sterility characters of plants and application of the gene. Specifically, the thermo-sensitive male sterility gene is a newly identified gene, mutation of a gene coding region of the thermo-sensitive male sterility gene causes generation of thermo-sensitive male sterility characters, and a new material is provided for Chinese cabbage male sterility molecular mechanism research and breeding.

The application belongs to the technical field of microorganisms, and particularly relates to an Escherichia coli resistant to low pH and application thereof. The Escherichia coli LZ1 has been sent to the China Center for Type Culture Collection, and the preservation date is November 11, 2024, and the preservation number is CCTCC NO: M 20242513. The mutS gene coding a DNA mismatch repair protein in the genome of the Escherichia coli MG1655 is knocked out, and based on the adaptive evolution breeding technology, the Escherichia coli with excellent low pH resistance is obtained. It is also accidentally found that the Escherichia coli also has good resistance in an alkaline environment, so a strain of Escherichia coli resistant to low pH and having broad-spectrum pH resistance is obtained. Therefore, the Escherichia coli LZ1 can be used as a potential chassis cell for synthesis of different compounds, and has good practical application value.

The invention discloses an overexpression vector of LAMP2B and application thereof, which can effectively delay the disease progress of DMD, and comprises: (a) an AAV vector which is an adeno-associated virus vector and has high muscle tissue targeting and low liver tropism; (b) a CMV (cytomegalovirus) promoter, wherein the CMV promoter is a cytomegalovirus promoter; (c) a coding sequence of an hLAMP2B gene, namely a coding sequence of a human LAMP2B gene, and the coding sequence of the hLAMP2B gene is operably linked to the CMV promoter; wherein the vector is configured to overexpress LAMP2B protein in muscular tissue, and the vector is self-complementary AAV, namely scAAV, the concept is ingenious, the novel AAV vector with high muscle transformation efficiency is utilized to deliver a complete LAMP2B gene (LAMP2B is a gene related to autophagy regulation) into muscle cells of all organs of a mouse (AAV-hLAMP2B for short), and the autophagy regulation function of the mouse is improved. The muscle tissue function of the mouse DMD disease model is comprehensively assisted to recover, and the disease progress of the DMD is delayed.

The invention provides an application of a CsSCPL36 gene, an encoded protein or a derivative thereof in regulating resistance of plants to anthracnose, and belongs to the technical field of biology. The invention provides a CsSCPL36 gene, an encoding protein of the CsSCPL36 gene or an application of a gene derivative containing the CsSCPL36 gene in regulation and control of resistance of plants to anthracnose and / or cultivation of anthracnose-resistant plant varieties. The CsSCPL36 gene or the CsSCPL36 gene coding protein can be used for improving the resistance of plants to anthracnose through forward regulation and control. According to the embodiment of the invention, by screening the influence of overexpression of different genes on anthracnose resistance, a result shows that overexpression of the CsSCPL36 gene can effectively improve the anthracnose resistance of the citrus, and a new way is provided for improving the resistance of the citrus to colletotrichum gloeosporioides.

The invention discloses a molecular marker related to disease resistance of ricefield eel and application of the molecular marker, and relates to the biological field of aquaculture and molecular marker screening. According to the invention, SNP loci in SFRP2 and TC1A gene coding regions in a ricefield eel group are screened through sequence alignment, and the two genes and a combination thereof are subjected to genetic typing; and comparing the differences of the disease resistance of the genotypes, and screening to obtain the genotype with the strongest disease resistance and the combination thereof, thereby providing an effective molecular marker for detecting the disease resistance of the ricefield eel. According to the monopterus albus disease resistance screening method developed based on the molecular marker, monopterus albus varieties with excellent disease resistance can be screened by utilizing SNP differences of disease-resistant genes, and then the monopterus albus varieties are used for disease resistance breeding. Effective technical support is provided for breeding of disease-resistant ricefield eel varieties.

The application discloses application of a rice metal tolerance protein coding gene OsMTP7 The rice metal tolerance protein coding gene OsMTP7 has the accession number of AK241225.1 in Genbank. The gene coding protein has zinc transport activity, can increase the zinc content of rice root system and above-ground part, and significantly increases the accumulation of zinc in rice grains.

The invention belongs to the technical field of drilling fluid deironing equipment, and provides a drilling fluid demagnetizing device parameter optimization method based on a genetic algorithm and a related device.The method comprises the steps that structure and process parameters of a drilling fluid demagnetizing device are obtained and converted into individual codes of the genetic algorithm, design parameters of the drilling fluid demagnetizing device are obtained and serve as gene codes, and the genetic algorithm is used as the individual codes of the drilling fluid demagnetizing device; each individual represents a device configuration parameter; constructing a fitness function based on the adsorption efficiency, the processing capacity and the cost of the drilling fluid demagnetizing device; and based on the fitness function, evaluating the fitness of each individual, performing selection, crossover and mutation operation on the population to generate a new generation of individuals, and when a predetermined evolution algebra is reached or the fitness reaches a predetermined threshold, completing optimization. Various parameters are allowed to be adjusted according to actual conditions by adopting a parameter optimization algorithm so as to adapt to different types of fluids containing magnetic substances, the flexibility is high, and the application field is wider.

This invention discloses an encoding method and system based on the projection constant C = 0.0017 and the digital gene 428571. The method determines the projection dimension parameters using the four-constant formula C = (2^N - 2^(N-2)) / 7 × 10^ exponent, introduces the digital gene sequence 428571 as the information encoding benchmark, achieves dimension mapping through binary-to-octal conversion, and establishes an 18-bit octal identity gene code generation model. This invention can be applied to proactive digital identity authentication systems, improving computational accuracy and information security through projection constant correction.

The invention discloses a method for improving salt resistance of plants. According to the method, an LSU3 gene coding sequence shown in SEQ ID NO: 1 is introduced into a plant cell and an LSU3 protein is expressed, so that the viability and the growth condition of the plant in a salt stress environment are remarkably enhanced. The invention further provides a recombinant expression vector containing the gene, a construction method of the recombinant expression vector and a specific application scheme of the recombinant expression vector in improvement of the salt resistance of arabidopsis thaliana, and effective gene resources and technical means are provided for salt-resistant breeding of crops.

The invention discloses an application of protein MoUPE11 in reducing pathogenicity of magnaporthe oryzae. A knockout vector of a gene for coding the protein MoUPE11 is constructed to obtain a knockout mutant MoUPE11, and pathogenicity analysis is performed on the knockout mutant MoUPE11 to find that compared with a wild type control, the pathogenicity of the knockout mutant MoUPE11 is remarkably reduced, and the pathogenicity of a complement mutant MoUPE11-com of the knockout mutant MoUPE11 is recovered to the wild type level, so that the protein MoUPE11 is related to the pathogenicity of pyricularia grisea, and the application has the advantages that the application is simple, the application is convenient, and the application is easy to popularize. The pathogenicity of the protein MoUPE11 can be reduced by inhibiting expression of the protein MoUPE11 in magnaporthe oryzae, so that the morbidity of the rice blast is reduced, the control of the rice blast is realized, and the adverse effect of the rice blast on rice is reduced. In addition, the protein MoUPE11 can be used as a target for development of green rice blast prevention and control products.

The invention belongs to the technical fields of immunotherapy, bioengineering, gene therapy and cell therapy, and provides a preparation method of an enhanced CAR-T cell for overexpressing LYAR, and the enhanced CAR-T cell and application thereof in order to solve the problems that the killing function of the CAR-T cell in solid tumor treatment is limited, the durability is poor, and function depletion is likely to occur. CDNA of the LYAR gene is cloned to an overexpression plasmid vector and packaged into lentivirus, T cells are jointly infected by the lentivirus and the CAR expression virus, the LYAR gene is forced to express under the driving of an EF-1alpha promoter, and stable overexpression of the LYAR in the CAR-T cells is achieved; and the CAR is a CAR targeting EGFR (epidermal growth factor receptor) and CD19. According to the present invention, the gene coding LYAR and the CAR gene are simultaneously transferred into the T cell through the lentivirus infection technology, and are compulsively expressed under the action of the EF1 alpha promoter, such that the anti-tumor function of the CAR-T cell can be significantly enhanced, and the activation and killing functions of the immune cell can be improved;

The invention discloses a preparation method of pichia pastoris expression secreting type deglycosylation alkaline phosphatase, and belongs to the technical field of biological engineering. According to the invention, the GS115-BIAP II pichia pastoris genetically engineered bacterium capable of secreting and expressing bovine intestine alkaline phosphatase BIAP II is prepared by using a conventional method. The method comprises the following steps: carrying out site-directed knockout on an ALG3 gene for coding alpha-1, 3-mannosyl transferase and an OCH1 gene for coding alpha-1, 6-mannosyl transferase by using a CRISPR-Cas9 (Clustered Regularly Interspaced Short Palindromic Repeats-associated Protein 9) technology to prepare a glycosylation gene defect type engineering bacterium GS115-delta ALG3-delta OCH1-BIAP II. The secretory expression BIAP II is deglycosylated alkaline phosphatase, and the specific activity of the secretory expression BIAP II is close to that of natural BIAP II. The deglycosylated alkaline phosphatase can improve the antibody coupling efficiency and marking uniformity under specific conditions. The method has remarkable advantages in chemiluminescence diagnosis, high-sensitivity immunodetection and development of multifunctional biological coupling reagents.

The invention discloses an application of a transcription factor ChZF7 of a basophilic microalgae Chlorella sp. BLD in regulation and control of salt tolerance of microalgae. According to the invention, the coding gene of the transcription factor ChZF7 is transferred into chlamydomonas reinhardtii by using a transgenic technology after intron insertion and codon optimization, so that transgenic chlamydomonas reinhardtii is obtained. The salt tolerance of the chlamydomonas reinhardtii with the over-expressed ChZF7 gene is remarkably improved, the accumulation of neutral fat and active oxygen under the stress of 225mM salt is less than that of the chlamydomonas reinhardtii in a control group, the content of penetrating substance proline is increased, in addition, the change of cell components in the chlamydomonas reinhardtii under the stress of salt can be weakened by the over-expressed ChZF7 gene, and the salt tolerance of the chlamydomonas reinhardtii is improved. The transcription factor ChZF7 and the gene coded by the transcription factor ChZF7 can regulate and control the salt tolerance of the chlamydomonas reinhardtii, a foundation is laid for genetic improvement and directed molecular breeding of the microalgae, the transcription factor ChZF7 plays an important role in improvement of the salt tolerance of the microalgae, reutilization of salinized water resources is facilitated, and the transcription factor ChZF7 and the gene coded by the transcription factor ChZF7 have wide application prospects.

The invention discloses an SNP (Single Nucleotide Polymorphism) molecular marker related to a pig body length character and application, and belongs to the technical field of biology. The SNP molecular marker is located on a pig X chromosome, and an SNP site is located at the 467th basic group of a pig BHLHB9 gene coding region (A in ATG in the coding region is 1); the basic group of the SNP site is C / T missense mutation. The SNP molecular marker related to the pig body length character is disclosed for the first time, is used for distinguishing individuals with medium and short body lengths and individuals with long body lengths, is suitable for domestic pig breeds and foreign pig breeds, and is wide in pig breed application range. The method for detecting the genotype of the SNP molecular marker disclosed by the invention can be used for quickly and effectively predicting the body length of a pig in an early stage at low cost, has a wide application prospect in the aspect of improvement of a pig variety strain, and can obtain an excellent economic value.