2 results about "Mating type" patented technology

This invention relates to the field of edible fungi spawn production technology, and more particularly to a yellow-lineage morel mushroom containing two mating type genes. Morchella sp. This invention discloses a pure strain of morel mushrooms, its preparation method, strain products containing the pure strain, and its use in the cultivation of *Morchella asiatica*. The genome of this pure strain contains both mating type genes MAT1-1-1 and MAT1-2-1. The advantage of this invention is that by placing multiple spores in a liquid culture medium for co-germination, the primary hyphae carrying different mating type genes will naturally fuse, forming visible antagonistic lines at the colony interface. Hyphae are picked from the antagonistic line region, and combined with PCR screening of the mating type genes, to efficiently obtain double-mating type strains. Furthermore, during the primary culture stage, covering the material surface with sterilized soil and controlling the temperature at 18-24℃ can induce the hyphae to expand in a directional, parallel manner from disordered twisting, and promote the differentiation of sclerotium-related structures at the casing soil-bottle wall interface, thereby significantly improving the quality of the primary culture.

The invention discloses a specific primer pair for identifying mating types of monokaryotic strains of stropharia rugoso-annulata and an identification method, and belongs to the field of molecular detection. The mating type identification primer based on the stropharia rugoso-annulata can be used for performing mating type identification on the monokaryotic strain of the stropharia rugoso-annulata, and the identification method is simple, economic and efficient; by adopting the detection method provided by the invention, according to the identification result of the mating type of the monokaryotic strain, the assembled hybrid combination can be successfully hybridized, and the locking combination is detected, which shows that the detection accuracy of identifying the mating type of the stropharia rugosoannulata by the primer is high; by utilizing the mating type site specific primer combination, A1 or A2, B1 or B2 can be specifically detected, the variety of mating types can be clearly distinguished and judged, and the specificity is relatively high.