5 results about "Mating type" patented technology

The invention belongs to the technical field of bioengineering, and relates to industrial microorganism breeding, in particular to a high-yield ethyl acetate diploid / tetraploid saccharomyces cerevisiae strain as well as a construction method and application thereof. According to the research, a diploid / tetraploid saccharomyces cerevisiae strain is constructed on the basis of an existing high-yield ethyl acetate haploid yeast strain by utilizing a CRISPR / Cas9 mediated mating type conversion technology. And a series of diploid / tetraploid saccharomyces cerevisiae strains with the acetic acid and lactic acid tolerance and the ethyl acetate yield obviously improved compared with the starting strain are obtained. According to the series of strains, ethyl acetate is high in yield while the excellent alcoholic fermentation characteristic is kept in the fermentation process, white spirit fermented by the series of strains is more prominent in ester fragrance and grain fragrance, weaker in foreign flavor and better in taste on the basis of guaranteeing the original style, a new thought is provided for the breeding mode of yeast strains, and the method is worthy of popularization and application. And a reference is provided for production optimization of ethyl acetate in a Baijiu fermentation process.

The invention relates to a morchella monospore hybridization breeding method, which belongs to the technical field of strain breeding, and comprises the following steps: (1) selecting morchella, obtaining a tissue isolation strain through a tissue isolation method, and obtaining a monospore isolation strain through a monospore isolation method; (2) respectively carrying out mating type gene detection on genomes of the tissue isolation strain and the single spore isolation strain; (3) in the same species, respectively selecting single spore isolation strains only carrying MAT1-1-1 mating type genes and single spore isolation strains only carrying MAT1-2-1 mating type genes for pairing hybridization; and (4) selecting the successful single spore hybrid combination and the corresponding two parent strains to perform cultivation test and agronomic character evaluation, and selecting a hybrid combination with yield and disease resistance superior to those of a single parent. The invention provides a high-efficiency breeding scheme capable of being popularized, reveals a remarkable effect of a mating type gene hybrid combination on the aspects of increasing the yield of morchella esculenta and enhancing disease resistance, and verifies positive regulation and control of mating type gene complementation on the yield and stress resistance.

This invention relates to the field of edible fungi spawn production technology, and more particularly to a yellow-lineage morel mushroom containing two mating type genes. Morchella sp. This invention discloses a pure strain of morel mushrooms, its preparation method, strain products containing the pure strain, and its use in the cultivation of *Morchella asiatica*. The genome of this pure strain contains both mating type genes MAT1-1-1 and MAT1-2-1. The advantage of this invention is that by placing multiple spores in a liquid culture medium for co-germination, the primary hyphae carrying different mating type genes will naturally fuse, forming visible antagonistic lines at the colony interface. Hyphae are picked from the antagonistic line region, and combined with PCR screening of the mating type genes, to efficiently obtain double-mating type strains. Furthermore, during the primary culture stage, covering the material surface with sterilized soil and controlling the temperature at 18-24℃ can induce the hyphae to expand in a directional, parallel manner from disordered twisting, and promote the differentiation of sclerotium-related structures at the casing soil-bottle wall interface, thereby significantly improving the quality of the primary culture.

The present invention belongs to the technical field of molecular breeding of edible fungi, and specifically discloses a molecular marker for identifying the mating type of Agrocybe poplar and its application. The mating type is A1, A2, B1 or B2, the molecular marker of mating type A1 is hda1; the molecular marker of mating type A2 is hda2; the molecular marker of mating type B1 is prb1; and the molecular marker of mating type B2 is prb2. The amino acid sequence of hda1 protein is shown in SEQ ID No.1; the amino acid sequence of hda2 protein is shown in SEQ ID No.5; the amino acid sequence of prb1 protein is shown in SEQ ID No.9; and the amino acid sequence of prb2 protein is shown in SEQ ID No.13. By using the molecular marker provided by the present invention, the mating type typing can be identified by molecular detection means in the breeding of Agrocybe poplar and its application is shortened, the breeding process is shortened, and the selection of excellent germplasm is accelerated.

The invention discloses a specific primer pair for identifying mating types of monokaryotic strains of stropharia rugoso-annulata and an identification method, and belongs to the field of molecular detection. The mating type identification primer based on the stropharia rugoso-annulata can be used for performing mating type identification on the monokaryotic strain of the stropharia rugoso-annulata, and the identification method is simple, economic and efficient; by adopting the detection method provided by the invention, according to the identification result of the mating type of the monokaryotic strain, the assembled hybrid combination can be successfully hybridized, and the locking combination is detected, which shows that the detection accuracy of identifying the mating type of the stropharia rugosoannulata by the primer is high; by utilizing the mating type site specific primer combination, A1 or A2, B1 or B2 can be specifically detected, the variety of mating types can be clearly distinguished and judged, and the specificity is relatively high.