82 results about "Mating type" patented technology

Methods are described for detecting protein-protein interactions, among two populations of proteins, each having a complexity of at least 1,000. For example, proteins are fused either to the DNA-binding domain of a transcriptional activator or to the activation domain of a transcriptional activator. Two yeast strains, of the opposite mating type and carrying one type each of the fusion proteins are mated together. Productive interactions between the two halves due to protein-protein interactions lead to the reconstitution of the transcriptional activator, which in turn leads to the activation of a reporter gene containing a binding site for the DNA-binding domain. This analysis can be carried out for two or more populations of proteins. The differences in the genes encoding the proteins involved in the protein-protein interactions are characterized, thus leading to the identification of specific protein-protein interactions, and the genes encoding the interacting proteins, relevant to a particular tissue, stage or disease. Furthermore, inhibitors that interfere with these protein-protein interactions are identified by their ability to inactivate a reporter gene. The screening for such inhibitors can be in a multiplexed format where a set of inhibitors will be screened against a library of interactors. Further, information-processing methods and systems are described. These methods and systems provide for identification of the genes coding for detected interacting proteins, for assembling a unified database of protein-protein interaction data, and for processing this unified database to obtain protein interaction domain and protein pathway information.

A hip-girdling pelvic sling device for maintaining a desired amount of tension surrounding a person's hips and pelvis to securely support and stabilize a pelvis that has been fractured. Areas of mating types of fastener material such as mating hook-bearing fastener material and loop pile fastener material are arranged on the device to enable a strap to be secured at various effective lengths to provide a wide range of adjustability to make the device useful for persons of various sizes.

The invention discloses an application of protein acetylation enzyme SIRT1 (Silent Mating type Information Regulation 2Homolog1), namely promoting proliferation and delaying senility of mesenchymal stem cells. The experimental result shows that the SIRT1 is needed by the in vitro proliferation of the mescenchymal stem cells, and the condition is verified by MSC (mesenchymal stem cells from marrow) and MSC from fat, and the proliferation and the senility of the MSC can be regulated by regulating the expression level of SIRT1 in the MSC. The invention has very important significances on the determination of the regulation mechanism of the proliferation and the senility of the MSC, the prolonging of the service life of the MSC, and the histocyte substitution and repairing treatment based on the MSC, and is broad in application prospect.

The invention provides a method for performing mating type gene identification on a basidiospore monocaryon strain which is separated from different hypsizigus marmoreus strains. In the identifying process, in particular application of a flushing reaction, a palisade reaction and transplanting, the method can primarily identify the mating type gene of the hypsizigus marmoreus intuitively and quickly without other instruments and equipment. When the method is applied in cross breeding, the mating type gene of basidiospore monocaryon parent and progeny strains can be identified conveniently so as to improve the purposiveness and the effectiveness of the cross breeding process. The adopted method can shorten the time for identifying the mating type gene for 5 to 10 days, reduce the number of comparing-screening strains by 75 percent theoretically in cross breeding, improve the fecundity, and is more convenient and efficient particularly in application of multiple cross breeding of multiple strains. Then, the method selects the compatible basidiospore monocaryon strain having clamp connection according to the identification result to carry out biparental crossing, and screens hybrid strains according to growth speed, growth potential and economical character index, thereby obtaining stable, high yield and high quality hybrid strains.

The present invention provides a genetic method for tethering polypeptides to the yeast cell wall in a form accessible for binding to macromolecules. Combining this method with fluorescence-activated cell sorting provides a means of selecting proteins with increased or decreased affinity for another molecule, altered specificity, or conditional binding. Also provided is a method for genetic fusion of the N terminus of a polypeptide of interest to the C-terminus of the yeast Aga2p cell wall protein. The outer wall of each yeast cell can display approximately 104 protein agglutinins. The native agglutinins serve as specific adhesion contacts to fuse yeast cells of opposite mating type during mating. In effect, yeast has evolved a platform for protein-protein binding without steric hindrance from cell wall components. As one embodiment, attaching an scFv antibody fragment to the Aga2p agglutinin effectively mimics the cell surface display of antibodies by B cells in the immune system for affinity maturation in vivo. As another embodiment, T cell receptor mutants can be isolated by this method that are efficiently displayed on the yeast cell surface, providing a means of altering T cell receptor binding affinity and specificity by library screening.

The invention relates to a method for distinguishing mating types of four varieties in black morchella group and a primer pair, and belongs to the field of biotechnology. The method comprises the following steps: extracting the DNA of a strain to be detected as a template, performing PCR amplification through the primer pair P7-2F / P7-2R or P8-5F / P8-5R to obtain a strain of MAT1-1 mating type of an expected band, performing PCR amplification through the primer pair P10-1F / P10-2R or P10-2F / P10-3R to obtain a strain of MAT1-2 mating type of an expected band, wherein the strain which can be detected by the two expected amplification bands is of two mating types. The four primers can be general to the mating type detection of the four varieties of morchella. A detection result of the method is high in specificity, and the method is stable, reliable and quick, and is applicable to researches on selection, cultivation, mating type genes and system development of strains of the morchella.

The invention provides a method for authenticating the mating type of eleven varieties in a black morchella group. The method includes the steps of firstly, the strain total DNA of a to-be-authenticated strain is extracted to serve as the PCR amplification template; secondly, a primer pair MAT1-1L / MAT1-1R is used to perform PCR amplification to obtain an expected-band strain which indicates that the strain contains the MAT1-1 mating type, a primer pair MAT1-2L / MAT1-2R to perform PCR amplification to obtain an expected-band strain which indicates that the strain contains the MAT1-2 mating type, and the fact that the strain only contains a male parent or a female parent is indicated if only the strain of one mating type is detected, and the strain is poor in fruiting ability or incapable of fruiting; the strains with two expected amplification bands capable being detected have the male parent mating type and the female parent mating type and have fruiting ability. By the primers and detection method for detection the mating type genes of morchella, the high yield and stable yield reliability during morchella cultivation can be increased greatly. The molecular marker of the mating type genes is simple, convenient, easy to operate and applicable to the morchella mating type gene authentication and cultivation, strain breeding, species identification, sexual reproduction evolution and phylogenic studies.

Methods are described for detecting protein-protein interactions, among two populations of proteins, each having a complexity of at least 1,000. For example, proteins are fused either to the DNA-binding domain of a transcriptional activator or to the activation domain of a transcriptional activator. Two yeast strains, of the opposite mating type and carrying one type each of the fusion proteins are mated together. Productive interactions between the two halves due to protein-protein interactions lead to the reconstitution of the transcriptional activator, which in turn leads to the activation of a reporter gene containing a binding site for the DNA-binding domain. This analysis can be carried out for two or more populations of proteins. The differences in the genes encoding the proteins involved in the protein-protein interactions are characterized, thus leading to the identification of specific protein-protein interactions, and the genes encoding the interacting proteins, relevant to a particular tissue, stage or disease. Furthermore, inhibitors that interfere with these protein-protein interactions are identified by their ability to inactivate a reporter gene. The screening for such inhibitors can be in a multiplexed format where a set of inhibitors will be screened against a library of interactors.

The invention relates to the application of SIRT1 (Silence Information Regulator Tuin1) to prepare medicine used for regulating down the expression of cyclin D1. Particularly, the medicine is of a delegiert protein type, and the form of the medicine can be any form suitable for the application of protein medicine. The medicine is a reorganization nucleic acid construction body capable of expressing SIRT1 protein. The expression carrier of the medicine can be any expression carrier which is suitable for gene therapy and comprises a virus carrier, such as a reorganization retrovirus carrier, an adenovirus carrier, an adeno-associated virus carrier, a herpes simplex virus carrier, a vaccinia virus carrier, and the like and a nonviral carrier, such as a bacteriophage carrier, a replicon carrier, and the like.