43results about How to "Increase carotenoid content" patented technology

The cauliflower (Brassica oleracea L. var. botrytis) Or gene is a semi-dominant, single-locus mutation. It induces the accumulation of high levels of beta-carotene in various tissues that are normally devoid of carotenoids, turning them orange. Using a map-based cloning strategy, we identified a single gene representing Or and successfully verified its identity by functional complementation in the wild type cauliflower. The Or gene encodes a plastid membrane protein containing the DnaJ zinc figure domain. A likely gain-of-function mutation from a 4.3-kb retrotransposon insertion in the Or allele confers the orange phenotype in the mutant. Southern blot analysis revealed that Or is a single-copy sequence in the cauliflower genome. High level of expression of the Or gene and the protein was found in very young leaves, curds, and flowers at comparable abundance between wild type and the Or mutant. Or likely functions in regulating the differentiation of some non-photosynthetic plastids into chromoplasts, which provide the deposition “sink” for carotenoid accumulation. Successful demonstration of Or in conferring carotenoid accumulation in potato tubers indicates its potential use to improve the nutritional value in staple crops.

The invention discloses a bacillus indius fermentation culture method which is characterized by comprising the following steps: inoculation of bacillus indius into a medium with the pH of 7-7.5; wherein the inoculation size is 1-2%, the culture temperature is 25-30 DEG C, the liquid filling volume is 20-40% of a container, the table concentrator rotating speed is 150-170r / min, the culture time is 48 hours, and the medium is a sea water solution with the soybean meal content of 0.2-0.4g / 100mL, the wheat bran content of 0.2-0.4g / 100mL and the yeast extract content of 0.1g / 100ml. The bacillus indius fermentation culture method is simple, low in cost, and suitable for promotion and application.

The invention relates to the field of nucleic acids, in particular to a circRNA and its application. More specifically, the present invention provides a sequence of circRNA PSY1-circ1 shown in SEQ ID NO: 1, a pair of primers for amplifying the circRNA PSY1-circ1, and an expression vector comprising the circRNA PSY1-circ1. By means of the circRNA PSY1-circ1 of the present invention, the regulation of plant phytoene synthase 1 gene (PSY1) expression, plant carotenoid content and tomato fruit color development can be achieved.

The invention belongs to the field of algae culture, particularly relates to the culture of nostoc sphaeroides, in particular to a production method for golden yellow nostoc sphaeroides. The production method comprises the following steps: 1) seed manufacture: selecting a nostoc sphaeroides parent body, carrying out sterilization processing to obtain an inoculum, putting the inoculum into a sterilized first culture medium to be subjected to aerated culture; 2) amplification culture: after the diameter of the nostoc sphaeroides is 0.5-1.0mm, according to a ratio of a nostoc sphaeroides cultureobtained in 1) to a first induction culture medium of 1 to (5 to 50), putting the nostoc sphaeroides into a culture container with the first induction culture medium to be subjected to open culture; 3) induction: transferring the nostoc sphaeroides of which the diameter is 1.5-2.5mm to a second induction culture medium to be subjected to illumination culture for 30-60 days, and thus obtaining thegolden yellow nostoc sphaeroides of which the diameter is 4-6mm. Nutrition salt, light intensity and light quality are regulated and controlled, the ratio of carotenoid in nostoc sphaeroides cells isimproved, so that the nostoc sphaeroides is golden yellow, the category of a nostoc sphaeroides product is enriched, and market requirements are met.

The invention relates to a strain for cultivating coccus, in particular to a coding gene of a chlorella endophyte and a specific primer for amplification thereof, belonging to the field of biotechnology. A gene encoding the chlorella endophyte, the nucleotide sequence of which is shown in SEQ ID No.1. The Chlorella endophyte is a bacterium of the genus Bacillus ( Microbacterium saccharophilum ) strain S395‑2, which was deposited in the General Microorganism Center of China Committee for the Collection of Microorganisms on July 30, 2018, with the number CGMCC No.16182. The application of the chlorella endophyte in promoting the growth of chlorella. Chlorella endophyte S395‑2 can be used as a new microbial resource to promote the growth of microalgae and improve the quality of microalgae growth.

The present invention discloses a cordyceps militaris cultivation culture media and a culture method, wherein the cordyceps militaris cultivation culture media is formed by mixing a solid matrix and a nutrient solution according to a mass ratio of 1:(0.5-1.5), the solid matrix is formed by mixing 95-99% by mass of grain (such as naked oats) and 1-5% by mass of silkworm pupa powder, and based on the sunlight lamp irradiation, at the primordium differentiation and fruit body development stage, the blue light lamp belt is adopted to supplement the Led blue light irradiation, and the fruit body sample is subjected to hot wind low temperature drying or freeze drying. According to the present invention, the naked oats are adopted as the raw materials, and the culture condition is controlled, such that the produced cordyceps militaris fruit body product has characteristics of high carotenoid content, dark yellow or orange color, good appearance and high commodity value, and the new utilization approach is developed for the naked oats.