43results about How to "Increase carotenoid content" patented technology

The present invention relates to a crystalline carotenoid compound, such as β-carotene, with a purity of at least 95% and with substantially no solvent enclosed in the crystal lattice. The present invention further describes a process to prepare such a highly pure crystalline carotenoid compound from microbial biomass, without the use of a solvent extraction and / or an anti-solvent crystallization process.

A method for producing probiotic feed for animals by fermenting high-fiber agricultural and sideline products with complex bacteria. , and / or 0-1.0% calcium chloride and / or 0-1.0% magnesium sulfate) are mixed in proportion and then steamed with water; after the steaming is completed, cool to 20-40°C and inoculate Neurostria fortis and Lactobacillus plantarum is solid-state fermented at 20-40°C for 2-5 days; the fermented feed is dried at 40°C-70°C and crushed to obtain a probiotic feed product rich in carotenoids. The method has the advantages of high production efficiency, low cost and good benefit, and while solving the problem of environmental pollution, it also relieves the pressure of serious shortage of energy and protein feed sources.

The invention discloses a method for enhancing weak light resistance of cucumbers by spraying compound bacterial manure on foliage, which relates to a method for enhancing illumination adversity resistance of crops. The bacterial strains are Chlorobium limicola SH II-4 and Rhodopseudomonas palustris SH II-5. The culture medium for preparing the microbial inoculum comprises 1.0g of (NH4)2SO4, 0.2g of MgSO4.7H2O, 3.0g of NaHCO3, 0.5g of K2HPO4 and 0.1g of NaCl; and the lighting culture is carried out for 4-5 days in the anaerobic environment to obtain the microbial inoculum of which the concentration is 1-2*108 viable bacteria / ml. The application method of the compound bacterial manure is realized by spraying the compound bacterial manure on the cucumber foliage in each growth period. The method has the characteristics of high safety, high efficiency, simple operation and low cost, effectively relieves the phenomena of weak leaf color, weak photosynthesis, retarded growth and the like caused by insufficient illumination, and obviously enhances both the yield and the quality; and the cucumbers are deep green in color.

The invention discloses a cultivating method of a new breeding strain of pinctada martensii with high carotenoid content. The cultivating method comprises the basic steps of parent selection, population construction, larval cultivation, sea area breeding and multi-generation population selection, and the new breeding strain of pinctada martensii with the high carotenoid content can be cultivated; for the obtained new strain, prismjatic layers of 96% of individuals are golden yellow, pallia, adductor muscles and gill filaments of 96% of the individuals are golden yellow, and the carotenoid content in pinctada martensii is 2-5 times that of control population. The new strain of pinctada martensii with the high carotenoid content is cultivated through a population selection breeding technology, obvious golden yellow is achieved, and the carotenoid content in pinctada martensii is high; the method is easy to popularize in production units and has a significant practical value and economic value.

The invention relates to Blakeslea trispora (B. trispora) strains producing at least 0.3 g / l lycopene, when co-cultivated for at least three days with a suitable strain of Blakeslea trispora of the opposite mating type in a suitable medium in the absence of an exogenous cartenogenesis inhibitor, to a natural efficient production process of lycopene by these strains, to a growth medium highly suited for this process and to an isolation process suited for any carotenoid compound.

The present invention discloses a cordyceps militaris cultivation culture media and a culture method, wherein the cordyceps militaris cultivation culture media is formed by mixing a solid matrix and a nutrient solution according to a mass ratio of 1:(0.5-1.5), the solid matrix is formed by mixing 95-99% by mass of grain (such as naked oats) and 1-5% by mass of silkworm pupa powder, and based on the sunlight lamp irradiation, at the primordium differentiation and fruit body development stage, the blue light lamp belt is adopted to supplement the Led blue light irradiation, and the fruit body sample is subjected to hot wind low temperature drying or freeze drying. According to the present invention, the naked oats are adopted as the raw materials, and the culture condition is controlled, such that the produced cordyceps militaris fruit body product has characteristics of high carotenoid content, dark yellow or orange color, good appearance and high commodity value, and the new utilization approach is developed for the naked oats.

The invention discloses a method for improving salt tolerance of peppermint by spraying microbes to blade surfaces, which relates to a method for improving soil stress resistance of crops, wherein the microbes are Rhodopseudomonas palustris SHII-3, and a culture medium prepared from a microbial inoculum comprises 0.02g of CaCl2, 1.0g of NH4Cl, 0.2g of K2HPO4, 0.2g of MgCl, 2.0g of NaCl, 30.05mg of NaHCO3, 3.0g of sodium acetate, 0.01g of yeast extract and 1000ml of water, wherein the PH of the culture medium is neutral, and the culture medium is sterilized for 30 min at the temperature of 121 DEG C. The microbes are cultured for 4-5 days under the light aerobic condition to obtain the microbial inoculum the concentration of which is 1-2*108 viable bacteria / ml. The microbial inoculum is sprayed to the blade surfaces at different growth periods after the peppermint is cut for the first time. The invention has the characteristics of safety, high efficiency, simple operation and low cost and effectively relieves the phenomena of plant wilting, slow growth and the like caused by high soil concentration; the content of peppermint camphor and menthone in blades is high.

The invention belongs to the technical field of plant genetic engineering, and particularly relates to a method for improving cottonseed nutritional quality and application thereof. The technical purpose that a new choice is provided for improving the cottonseed carotenoid content and improving cottonseed nutritional quality is achieved. According to the technical scheme, a seed-specific expression promoter is built to drive the plant expression vector of a phytoene synthetase gene PSY, cotton is transformed, the carotenoid content in cottonseeds is improved, and the PSY gene is the cotton GhPSY2 gene. After the target gene is regulated through the method, the carotenoid content in mature cottonseeds is significantly improved. The method is easy and convenient to implement and remarkable in effect, and has the application prospect of producing huge economic benefits.

A method for improving tobacco incense quality by inducing carotenoid synthesis is characterized in that carotenoid synthesis revulsants CPTA and MPTA are applied on the surface of tobacco strains or tobacco leaves to participate in the processes of absorption, decomposition, conversion and synthesis of the tobacco strains, thereby improving the carotenoid content in the tobacco leaves, the presentation quality of the tobacco leaves and the incense quality. The method has the following advantages and effects: carotenoid synthesis revulsants with specific concentrations are applied by top pruning or after tobacco leaf collection for having a regulation function on related enzymic metabolism in the carotenoid synthesis and promoting the improvement of carotenoid content in the tobacco leaves. Tests prove that the application of the two carotenoid synthesis revulsants CPTA and MPTA in the invention can respectively improve the modulated carotenoid content of the tobacco leaves by 32% and 58%, and sensory smoking results show that the incense quality of tobacco leaves can be obviously improved and the irritation and mixed gas are reduced.

The invention discloses a cucumber carotenoid phytoene synthetase gene. All cucumber carotenoids are synthesized by isoprenoid compounds or terpenoids. The length of the nucleotide sequence SEQ ID of the cucumber carotenoid phytoene synthetase gene is 1479bp, and the cucumber carotenoid phytoene synthetase gene has a specific nucleotide sequence. The gene-coded polypeptide of claim 1 comprises 422 amino acids, has the molecular weight of 47393.507Da, the isoelectric point of 6.545, the charge of 0.777 when the pH value is 7.0, and has a specific amino acid sequence. The recombinant plasmid pT-PSY of the gene of claim 1 has the length of 4515bp, comprises ampicillin selection markers, and has a specific nucleotide sequence. The invention is used for cloning cucumber carotenoid phytoene synthetase genes.

The invention belongs to the field of algae culture, particularly relates to the culture of nostoc sphaeroides, in particular to a production method for golden yellow nostoc sphaeroides. The production method comprises the following steps: 1) seed manufacture: selecting a nostoc sphaeroides parent body, carrying out sterilization processing to obtain an inoculum, putting the inoculum into a sterilized first culture medium to be subjected to aerated culture; 2) amplification culture: after the diameter of the nostoc sphaeroides is 0.5-1.0mm, according to a ratio of a nostoc sphaeroides cultureobtained in 1) to a first induction culture medium of 1 to (5 to 50), putting the nostoc sphaeroides into a culture container with the first induction culture medium to be subjected to open culture; 3) induction: transferring the nostoc sphaeroides of which the diameter is 1.5-2.5mm to a second induction culture medium to be subjected to illumination culture for 30-60 days, and thus obtaining thegolden yellow nostoc sphaeroides of which the diameter is 4-6mm. Nutrition salt, light intensity and light quality are regulated and controlled, the ratio of carotenoid in nostoc sphaeroides cells isimproved, so that the nostoc sphaeroides is golden yellow, the category of a nostoc sphaeroides product is enriched, and market requirements are met.

The cauliflower (Brassica oleracea L. var. botrytis) Or gene is a semi-dominant, single-locus mutation. It induces the accumulation of high levels of beta-carotene in various tissues that are normally devoid of carotenoids, turning them orange. Using a map-based cloning strategy, we identified a single gene representing Or and successfully verified its identity by functional complementation in the wild type cauliflower. The Or gene encodes a plastid membrane protein containing the DnaJ zinc figure domain. A likely gain-of-function mutation from a 4.3-kb retrotransposon insertion in the Or allele confers the orange phenotype in the mutant. Southern blot analysis revealed that Or is a single-copy sequence in the cauliflower genome. High level of expression of the Or gene and the protein was found in very young leaves, curds, and flowers at comparable abundance between wild type and the Or mutant. Or likely functions in regulating the differentiation of some non-photosynthetic plastids into chromoplasts, which provide the deposition “sink” for carotenoid accumulation. Successful demonstration of Or in conferring carotenoid accumulation in potato tubers indicates its potential use to improve the nutritional value in staple crops.

The invention makes public a preparing method for xanthophyll crystals with higher content of zeaxanthin from plant oleoresin. The current methods generally are to get quite pure crystal forms of xanthophyll or zeaxanthin, and they refer to several separation steps. The invention mixes the xanthophyll diester-containing plant oleoresins and food grade alcohol solvents to form even solution, and then soap-dissolve the solution under an alkaline environment; then replenish organic solvents and emulsifiers into the reaction solution and drop some alkali solution into the solution to make partial xanthophyll crystals be transformed to be zeaxanthin through epimerization reaction; after the reaction is finished, add the mixed solvents of alcohol solvent and water to separate out the crystals; use the method of centrifugation or filtration to get the crystals; wash the crystals several times with the mixed solution of deionized water and alcohols to remove the impurities among the crystals; recrystallize the gained crystals with absolute ethyl alcohol, and then dry the crystals to get the products. The invention can gain mixture crystals that contain xanthophyll and zeaxanthin at one time in quite high collection rate, and it is convenient for the followed product application.

The invention provides a method for increasing the content of carotenoid in patinopecten yessoensis, which is used for increasing the content of carotenoid in adductor muscles of scallops by inhibiting the expression quantity of protease having the function of degrading carotenoid in the patinopecten yessoensis. The method is performed by reducing the quantity of protease having an amino acid sequence of SEQ ID NO:1 in patinopecten yessoensis; and a RNA interference technology is used for inhibiting expression of a target gene and detecting variation of the expression quantity of the gene after interference and the content of carotenoid in adductor muscles of scallops, which proves that screened protease has the function of degrading the carotenoid in patinopecten yessoensis, and the content of carotenoid in adductor muscles of scallops can be increased by inhibiting the expression of the protease.

The invention discloses an application of protein AeZDS and encoding gene thereof in promoting accumulation and stress resistance of plant carotenoids. The invention provides a protein (a) or (b) as follows: (a) the protein composed of an amino acid sequence shown as sequence SEQ ID NO.2 in a sequence table, and (b) the protein with one or more substituted and / or deleted and / or added amino acid residue in the amino acid sequence shown as sequence SEQ ID NO.2 in the sequence table. When the encoding gene of protein is introduced into arabidopsis, the content of carotenoid in transgenic arabidopsis is obviously increased and the stress resistance of transgenic arabidopsis plant is promoted. The protein AeZDS and the encoding gene thereof related to biosynthesis and stress resistance of carotenoids have important theoretical significance and practical value in regulating biosynthesis and stress resistance of plant carotenoids.

The invention relates to a strain for culturing aegagropila linnaei, in particular to a coding gene of chlorella endophyte and a specific primer for amplifying the coding gene, and belongs to the technical field of biology. The nucleotide sequence of the gene for coding the chlorella endophyte is as shown in SEQ ID No.1. The chlorella endophyte is a strain S395-2 of microbacterium bacteria (Microbacterium saccharophilum), the strain is preserved in the China General Microbiological Culture Collection Center on 30 July 2018, and the number is CGMCC No.16182. The invention relates to an application of the chlorella endophyte in the respect of promoting growth of chlorella. The chlorella endophyte S395-2 can be used as novel microorganism resources for promoting growth of microalgae and improving the growth quality of the microalgae.

The invention relates to a method for applying photosynthetic bacteria to improve the tolerance of corn to oil and cadmium contaminated soil, belonging to the method for improving the tolerance of soil. The method comprises the following steps: spraying a diluted bacterial agent onto the soil before sowing corn seeds; diluting a bacterial agent, and soaking the corn seeds by the diluted bacterial agent; sowing the corn seeds; spraying the diluted bacterial agent; monitoring the content of chlorophyll in the corn seedling every week from the seedling stage: selecting typical corn seedlings in the cluster, and measuring the content of the chlorophyll in the fully-expanded leaves on the topmost joints of the corn seedlings; or spraying calcium chloride solution to the surfaces of the corn seedling once in the booting stage and the postulating stage respectively, diluting a stock photosynthetic bacterial agent, and spraying the stock photosynthetic bacterial agent once. The method is low in cost and safe in process and is environmentally friendly. Due to the adoption of the method, the content of chlorophyll and carotenoid in the corn seedlings are increased significantly, the corn seedlings are tall and have thick stems, the dry weight and the raw weight of the corn seedlings are improved greatly, the contents of proline, POD and chelating peptide and other resistance indexes of the corn seedlings are improved, the malondialdehyde is reduced, the corn can tolerate oil and cadmium contaminated soil, the physical and chemical properties of the soil are improved significantly, and the fertility of the soil is enhanced.

The invention discloses a breeding method of a high-concentration CO2-tolerant microalgae species. The breeding method comprises the following steps: 1) cultivating inoculated microalgae with the presence of less than 40% of CO2 and 20-500 [micro]moles of photons m-2s-1 at 15-40 DEG C under the conditions of a light-dark cycle of (1-24h) / (0-23h) and an apparent ventilation rate of 0.01-1.0cm / s for 2-10 days as an adaptive evolution culture cycle; 2) inoculating the microalgae which is cultivated in the step 1) once again, and cultivating by a new cycle under conditions same with that of the step 1) for 2-10 days; and 3) turning to a next culture cycle as methods prescribed within the step 2) by taking operations prescribed within the step 1) as a culture cycle, and repeating the process by 10-200 culture cycles, so that the CO2-tolerant microalgae species is obtained. The breeding method disclosed by the invention is applicable to the breeding of microalgae bio-fuel species; and meanwhile, the breeding method can also provide a basis for microalgae biological studies.

The invention relates to a foliar fertilizer for promoting yellowing of flue-cured tobacco and a use method. The foliar fertilizer is prepared from the following components in parts by weight and volume: 0.2 to 0.3 kg / L of monopotassium phosphate, 0.1 to 0.2 kg / L of sodium bicarbonate, 15 to 25mg / L of abscisic acid and 10 to 20mg / L of auxin. According to the technical scheme, the foliar fertilizercan effectively reduce the chlorophyll content in the later period of flue-cured tobacco and increase the carotenoid content, and yellowing of flue-cured tobacco in the later period of field production is obvious. And the ratio of yellow smoke in baking quality is obviously improved, the ratio of green smoke, green smoke and yellow smoke in micro-strip is reduced, and the flue-cured tobacco output value and economic benefit are remarkably increased.

The invention discloses a production method for a high-quality sporophore of cordyceps militaris(L.)Fr. allowing carotenoid and cordycepin contents to be simultaneously increased. A cultivation methodprovided by the invention divides a cultivation process of the sporophore of the cordyceps militaris(L.)Fr. into four different stages including spawn runing, primordium differentiation, sporophore growth and accumulation of metabolites, and temperature, light and humidity control are separately performed on the different stages. In the stages of the sporophore growth and the accumulation of themetabolites, based on white light irradiation, Led blue light irradiation is supplemented by utilizing a blue light strip, and sporophore samples are dried at low temperature by employing hot air or freeze-dried. By utilization of a conventional culture medium and through control of culture conditions by stages, a product of the sporophore of the cordyceps militaris(L.)Fr. is produced and obtained, allows carotenoid and cordycepin contents to be simultaneously increased, is deep yellow or orange in color, is good in appearance, and is high in commodity value, and the production method is simple and easy to implement, and can achieve high-quality production of the sporophore of the cordyceps militaris(L.)Fr..

The invention relates to a strain for culturing chlorella, in particular to a chlorella endophyte and an application thereof, which belong to the technical field of biology. The invention discloses the chlorella endophyte. The bacterial strain is a bacterial strain S395-2 of Microbacteriumsaccharophilum. The strain is preserved in China General Microbiological Culture Collection Center on July 30,2018, and a serial number of the strain is CGMCC No. 16182. According to the invention, Chlorella sp. is used as a research object, the dominant endophyte is separated and purified from chlorella, after classification and identification, a symbiotic system of chlorella and the chlorella endophyte is constructed artificially, and the growth promoting effect of endophyte in the chlorella symbioticsystem on chlorella is researched. The chlorella endophyte S395-2 can be used as a new microbial resource for promoting microalgae growth and improving microalgae growth quality.

The invention discloses a method for creating high-carotenoid sweet corn by using a gene editing technology, and belongs to the technical field of gene engineering. According to the method for creating the high-carotenoid sweet corn by using the gene editing technology, corn ZmSu1, ZmIsa2, ZmIsa3 and ZmZpu1 genes are subjected to gene editing at the same time through the CRISPR / Cas9 technology, mutant materials containing target gene mutation fragments are obtained through further screening, and the materials with sweetened grains and increased carotenoid content have important breeding value.

The invention discloses a bacillus indius fermentation culture method which is characterized by comprising the following steps: inoculation of bacillus indius into a medium with the pH of 7-7.5; wherein the inoculation size is 1-2%, the culture temperature is 25-30 DEG C, the liquid filling volume is 20-40% of a container, the table concentrator rotating speed is 150-170r / min, the culture time is 48 hours, and the medium is a sea water solution with the soybean meal content of 0.2-0.4g / 100mL, the wheat bran content of 0.2-0.4g / 100mL and the yeast extract content of 0.1g / 100ml. The bacillus indius fermentation culture method is simple, low in cost, and suitable for promotion and application.

The invention discloses a method for increasing the carotenoid content of corn kernels by using a gene editing technology, and belongs to the technical field of gene engineering. According to the method for improving the carotenoid content of the corn kernels by using the gene editing technology, disclosed by the invention, corn ZmIsa2, ZmIsa3 and ZmZpu1 genes are subjected to gene editing at the same time through a CRISPR / Cas9 technology, mutant materials containing target gene mutation fragments are obtained through further screening, and the materials with the carotenoid content improved in the corn kernels have important breeding value.

The invention belongs to the field of algae culture, particularly relates to the culture of nostoc sphaeroides, in particular to a production method for golden yellow nostoc sphaeroides. The production method comprises the following steps: 1) seed manufacture: selecting a nostoc sphaeroides parent body, carrying out sterilization processing to obtain an inoculum, putting the inoculum into a sterilized first culture medium to be subjected to aerated culture; 2) amplification culture: after the diameter of the nostoc sphaeroides is 0.5-1.0mm, according to a ratio of a nostoc sphaeroides cultureobtained in 1) to a first induction culture medium of 1 to (5 to 50), putting the nostoc sphaeroides into a culture container with the first induction culture medium to be subjected to open culture; 3) induction: transferring the nostoc sphaeroides of which the diameter is 1.5-2.5mm to a second induction culture medium to be subjected to illumination culture for 30-60 days, and thus obtaining thegolden yellow nostoc sphaeroides of which the diameter is 4-6mm. Nutrition salt, light intensity and light quality are regulated and controlled, the ratio of carotenoid in nostoc sphaeroides cells isimproved, so that the nostoc sphaeroides is golden yellow, the category of a nostoc sphaeroides product is enriched, and market requirements are met.

The invention relates to a strain for cultivating coccus, in particular to a coding gene of a chlorella endophyte and a specific primer for amplification thereof, belonging to the field of biotechnology. A gene encoding the chlorella endophyte, the nucleotide sequence of which is shown in SEQ ID No.1. The Chlorella endophyte is a bacterium of the genus Bacillus ( Microbacterium saccharophilum ) strain S395‑2, which was deposited in the General Microorganism Center of China Committee for the Collection of Microorganisms on July 30, 2018, with the number CGMCC No.16182. The application of the chlorella endophyte in promoting the growth of chlorella. Chlorella endophyte S395‑2 can be used as a new microbial resource to promote the growth of microalgae and improve the quality of microalgae growth.

The present invention discloses a cordyceps militaris cultivation culture media and a culture method, wherein the cordyceps militaris cultivation culture media is formed by mixing a solid matrix and a nutrient solution according to a mass ratio of 1:(0.5-1.5), the solid matrix is formed by mixing 95-99% by mass of grain (such as naked oats) and 1-5% by mass of silkworm pupa powder, and based on the sunlight lamp irradiation, at the primordium differentiation and fruit body development stage, the blue light lamp belt is adopted to supplement the Led blue light irradiation, and the fruit body sample is subjected to hot wind low temperature drying or freeze drying. According to the present invention, the naked oats are adopted as the raw materials, and the culture condition is controlled, such that the produced cordyceps militaris fruit body product has characteristics of high carotenoid content, dark yellow or orange color, good appearance and high commodity value, and the new utilization approach is developed for the naked oats.

The invention discloses key enzyme protein AePDS for biosynthesis of abelmoschus esculentus carotenoid and applications of an encoding gene of the key enzyme protein AePDS to increase of content of plant carotenoid and improvement of salt tolerance and drought resistance. The protein is as follows (a) or (b): (a), protein composed of an amino acid sequence shown in SEQ ID NO. 2; (b), protein whichis derived from the sequence SEQ ID NO. 2, associated with plant stress resistance and obtained by substituting and / or deleting and / or adding one or more amino acid residues in the amino acid residuesequence shown in SEQ ID NO. 2 in the sequence table. Experiments prove that when the encoding gene of the protein is introduced into arabidopsis thaliana, the content of carotenoid in the transgenicarabidopsis thaliana is obviously increased, and the salt tolerance and drought resistance of transgenic arabidopsis thaliana plants are simultaneously improved. Therefore, the protein AePDS associated with biosynthesis of abelmoschus esculentus carotenoid and stress resistance and the encoding gene of the protein have great theoretical significance and practical value in regulation of biosynthesis of the plant carotenoid as well as salt tolerance and drought resistance. The protein has broad application space and market prospect in the agricultural field.

The invention relates to marine organisms, and in particular relates to an improvement of a spirulina culture medium. The organic spirulina culture medium comprises water, baking soda and salt; the baking soda and the salt are added to the water; the salt is 0.018 to 0.04% in mass of the water; the amount of added baking soda is the amount meeting the requirement that the pH (Potential Of Hydrogen) of the spirulina culture medium ranges from 8.2 to 9.8; and the supernate of liquid methane, being 0.8 to 1.6% in mass of the water, is also added to the water. The organic spirulina culture medium does not influence the growth of spirulina and can increase the variety and content of vitamins and trace elements in the spirulina culture medium, so that the organic spirulina culture medium is more conducive to human health.