Cordyceps militaris cultivation culture media for increasing carotenoid content, and culture method

A technology of carotene and culture medium, which is applied in the culture medium and cultivation field of Cordyceps militaris with increased carotenoid content, can solve the problems of Cordyceps militaris without high carotenoid content, and achieve the effect of good appearance and high commodity value

Active Publication Date: 2015-02-25
HEBEI JIAZHEN AGRI TECH CO LTD
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

[0004] At present, there are many patents on the cultivation of Cordyceps militaris, but so far there is no patent on the cultivation of Cordyceps militaris with high carotenoid content, only the research on the extraction of carotenoids from Cordyceps militaris

Method used

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  • Cordyceps militaris cultivation culture media for increasing carotenoid content, and culture method

Examples

Experimental program
Comparison scheme
Effect test

Embodiment 1

[0034] Embodiment 1, the cultivation of the fruiting bodies of Cordyceps militaris with high carotenoid content

[0035] In this example, the Cordyceps militaris strain used is the Cordyceps militaris strain "Dongfang No. 9": purchased from Dezhou Dongfang Biotechnology Research Institute, Shandong Province.

[0036] One, the preparation of culture medium of Cordyceps militaris

[0037] Mix 297g oatmeal granules and 3g silkworm chrysalis powder evenly, divide them into 10 cultivation bottles, 30g / bottle, add 45g nutrient solution to each bottle, seal, sterilize at 121°C for 1 hour, and cool to room temperature for later use. Obtain the culture bottle that contains Cordyceps militaris culture medium.

[0038] 2. Preparation of seed solution

[0039] Prepare the seed liquid of Cordyceps militaris bacterial strain according to routine method, specifically as follows:

[0040] 1. Strain activation

[0041] The Cordyceps militaris strain "Dongfang No. 9" was inoculated on potat...

Embodiment 2

[0065] Embodiment 2, the cultivation of the fruiting bodies of Cordyceps militaris with high carotenoid content

[0066] In this example, the Cordyceps militaris strain used is the Cordyceps militaris strain "Megasporophthora militaris 09-888": purchased from Yatai Institute of Microbiology, Jinzhou, Liaoning.

[0067] One, the preparation of culture medium of Cordyceps militaris

[0068] Mix 285g naked oats and 15g silkworm chrysalis powder evenly, divide them into 10 cultivation bottles, 30g / bottle, add 45g nutrient solution to each bottle, seal, sterilize at 121°C for 1 hour, wait for cooling to room temperature for later use, and obtain Culture bottle containing Cordyceps militaris cultivation medium.

[0069] 2. Preparation of seed solution

[0070] The seed liquid of the Cordyceps militaris strain was prepared according to the conventional method, and the specific operation was referred to Step 2 of Example 1 to obtain the seed liquid.

[0071] 3. Inoculation and cult...

Embodiment 3

[0080] Embodiment 3, the cultivation of the fruiting bodies of Cordyceps militaris with high carotenoid content

[0081] In this example, the Cordyceps militaris strain used was the Cordyceps militaris strain "Minyuan No. 1": it was purchased from the Strain Production Center of Minyuan High-tech Institute, Sichuan Province.

[0082] One, the preparation of culture medium of Cordyceps militaris

[0083] Mix 290g of naked oats and 10g of silkworm chrysalis powder evenly, divide them into 10 cultivation bottles, 30g / bottle, add 45g of nutrient solution to each bottle, seal, sterilize at 121°C for 1 hour, and wait to cool to room temperature for later use, and obtain Culture bottle containing Cordyceps militaris cultivation medium.

[0084] 2. Preparation of seed solution

[0085] The seed liquid of the Cordyceps militaris strain was prepared according to the conventional method, and the specific operation was referred to Step 2 of Example 1 to obtain the seed liquid.

[0086]...

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Abstract

The present invention discloses a cordyceps militaris cultivation culture media and a culture method, wherein the cordyceps militaris cultivation culture media is formed by mixing a solid matrix and a nutrient solution according to a mass ratio of 1:(0.5-1.5), the solid matrix is formed by mixing 95-99% by mass of grain (such as naked oats) and 1-5% by mass of silkworm pupa powder, and based on the sunlight lamp irradiation, at the primordium differentiation and fruit body development stage, the blue light lamp belt is adopted to supplement the Led blue light irradiation, and the fruit body sample is subjected to hot wind low temperature drying or freeze drying. According to the present invention, the naked oats are adopted as the raw materials, and the culture condition is controlled, such that the produced cordyceps militaris fruit body product has characteristics of high carotenoid content, dark yellow or orange color, good appearance and high commodity value, and the new utilization approach is developed for the naked oats.

Description

technical field [0001] The invention relates to a culture medium and a culture method of Cordyceps militaris, in particular to a culture medium and a culture method of Cordyceps militaris with increased carotenoid content. Background technique [0002] Cordyceps militaris (L.) Link, also known as Cordyceps militaris, is a rare edible and medicinal fungus with high nutritional value and nourishing effect. "Compendium of Xinhua Materia Medica" stated that Cordyceps militaris "whole herb: sweet in taste, flat in nature. It is beneficial to the lungs and kidneys, nourishes the essence, stops bleeding and resolves phlegm." Modern pharmacological research has also confirmed that Cordyceps militaris is anti-tumor, anti-virus, and lowers blood pressure. and other functions. Because its nourishing effect and medicinal effect are similar to the rare but difficult to cultivate Cordyceps sinensis, it is gradually becoming an ideal substitute for Cordyceps sinensis. As a food, Cordycep...

Claims

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Application Information

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Patent Type & Authority Applications(China)
IPC IPC(8): C05G3/00A01G1/04
CPCA01G18/00C05F1/005C05F11/00
Inventor 温建桥
Owner HEBEI JIAZHEN AGRI TECH CO LTD
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