Encoding gene of Chlorella endophyte and specific primers for its amplification

A technology of chlorella and endophytes, applied in genetic engineering, plant genetic improvement, biochemical equipment and methods, etc., can solve the problems of neglecting microbial groups, not showing external characteristics and corresponding functions, etc.

Active Publication Date: 2021-07-27
JIAXING UNIV
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

Since Kloepper first proposed the concept of "plant endophytes", the research on plant endophytes at home and abroad has a history of more than 100 years, but because they do not show obvious external characteristics and corresponding functions , resulting in this microbial group being neglected by people for a long time

Method used

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  • Encoding gene of Chlorella endophyte and specific primers for its amplification
  • Encoding gene of Chlorella endophyte and specific primers for its amplification
  • Encoding gene of Chlorella endophyte and specific primers for its amplification

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Embodiment Construction

[0025] The technical solution of the present invention will be further specifically described below through specific examples. It should be understood that the implementation of the present invention is not limited to the following examples, and any modifications and / or changes made to the present invention will fall within the protection scope of the present invention.

[0026] In the present invention, unless otherwise specified, all parts and percentages are in weight units, and the equipment and raw materials used can be purchased from the market or commonly used in the field. The methods in the following examples, unless otherwise specified, are conventional methods in the art.

[0027] Example Isolation, purification and identification of Chlorella endophytes

[0028] 1 Experimental materials

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Abstract

The invention relates to a strain for cultivating coccus, in particular to a coding gene of a chlorella endophyte and a specific primer for amplification thereof, belonging to the field of biotechnology. A gene encoding the chlorella endophyte, the nucleotide sequence of which is shown in SEQ ID No.1. The Chlorella endophyte is a bacterium of the genus Bacillus ( Microbacterium saccharophilum ) strain S395‑2, which was deposited in the General Microorganism Center of China Committee for the Collection of Microorganisms on July 30, 2018, with the number CGMCC No.16182. The application of the chlorella endophyte in promoting the growth of chlorella. Chlorella endophyte S395‑2 can be used as a new microbial resource to promote the growth of microalgae and improve the quality of microalgae growth.

Description

technical field [0001] The present invention relates to a kind of strain used for culturing coccus, in particular to a coding gene of chlorella endophyte and specific primers for amplification thereof, belonging to the field of biotechnology. Background technique [0002] Plant endophytes are a new type of sustainable microbial resource with broad application prospects. Since Kloepper first proposed the concept of "plant endophytes", the research on plant endophytes at home and abroad has a history of more than 100 years, but because they do not show obvious external characteristics and corresponding functions , leading to the long-term neglect of this microbial group. It was not until the end of the 20th century that Stierle et al. isolated and screened an endophytic fungus capable of producing paclitaxel in the tissue of Taxus brevifolia, and this research was paid attention to and carried out in depth, and then became a research hotspot at home and abroad. Researchers h...

Claims

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Application Information

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Patent Type & Authority Patents(China)
IPC IPC(8): C12N15/31C12N15/11
CPCC07K14/195C12N15/11
Inventor 孙诗清赵永军胡长伟曹卫星刘娟孙辰
Owner JIAXING UNIV
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