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169 results about "Microalgae growth" patented technology
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Heterotrophic algal high cell density production method and system
InactiveUS20090209014A1Increase cell densityIncrease biomassUnicellular algaeBiofuelsHigh cellHigh density
A multiphase culturing process for high density heterotrophic microalgal growth uses crude glycerol as the primary carbon source and produces ω-3 fatty acids. The process uses multiphase growth conditions that decouple the phases of increasing cell density and increasing cell size and fatty acid production. The entire process is integrated with biodiesel production.
Owner:WASHINGTON STATE UNIV RES FOUND INC
Nitrogen-phosphorus wastewater treating method by sequencing batch reactor coupled photobioreactor
ActiveCN102336498AWater quality varies widelyMultistage water/sewage treatmentSequencing batch reactorHigh concentration
The invention relates to the field of environmental wastewater treatment, specifically to a nitrogen-phosphorus wastewater treating method by a sequencing batch reactor coupled photobioreactor. The method provided by the invention is characterized in that it comprises the following steps of: removing impurities and sediments of high concentration nitrogen-phosphorus wastewater and then treating the wastewater by using active sludge in the sequencing batch reactor, decanting a supernatant from the sequencing batch reactor, adding the supernatant into a membrane bioreactor for further aerobic treatment, filtering by ultrafiltration membrane in the membrane bioreactor to remove micro-suspension and bacteria, adding a generated ultrafiltration supernatant into a photobioreactor, disinfecting by ozone and adding algae filament as well as a few necessary nutrient salt, filling with carbon dioxide waste gas to perform microalgae cultivation, discharging a algae liquid after a microalgae growth cycle and adding the algae liquid into a flocculating settling pool for sedimentation, standardly discharging or reusing a supernatant liquor, compressing the concentrate algae liquid at the bottominto a microalgae cake by a plate and frame filter press. The invention has advantages of wide range of treated nitrogen-phosphorus wastewater water quality variation and production of economical microalgae with the utilization of nitrogen and phosphorus nutrients in wastewater as more as possible.
Owner:DALIAN DAKAI SEWAGE TREATMENT
Microalgae automatic counting method based on digital image processing
InactiveCN103955937AOvercome efficiencyOvercome speedImage analysisMicroscopic imageDigital signal processing
The invention discloses a microalgae automatic counting method based on digital image processing. Digitalized processing is conducted on a preprocessed picture through a matlab image processing method to extract a binary image, morphological processing is conducted on the image, and the amount of microalgae is calculated. The problems of heavy labor and measurement errors caused by manual counting of microalgae microscopic images at present are solved. Meanwhile, the reliable counting method and the reliable counting steps are provided for the difficult problem that due to the fact that the microalgae microscopic images are easily affected by illumination impurity interference, light-background dark objects and cytoadherence, the microalgae can not be accurately counted, and the microalgae automatic counting method has important significance in microalgae growth monitoring.
Owner:FUZHOU UNIV
Apparatus and cultivating method for scaled cultivation of microalgae
ActiveCN102206570AShort optical pathIncrease light receiving areaBioreactor/fermenter combinationsBiological substance pretreatmentsEngineeringPhotosynthetic Carbon Fixation
The invention relates to an apparatus and a cultivating method for scaled cultivation of microalgae. The apparatus comprises: an open-type track tank; a liquid outlet arranged on the open-type track tank; a liquid pump connecting to the liquid outlet, wherein a liquid inlet on the bottom of a photobioreactor is connected to the liquid outlet through the liquid pump; a liquid outlet tube on the top of the photobioreactor, wherein culture solution is delivered back to the open-type track tank through the tube. The cultivating method comprises the steps that: part of the culture solution in the open-type track tank is pumped into the bottom of the photobioreactor through the liquid pump; the culture solution is delivered from the top of the photobioreactor back to the open-type track tank, such that a circulation of culture solution is realized; the bottom of the photobioreactor is ventilated, and carbon is filled. With the apparatus and the method provided by the present invention, photosynthetic carbon fixation efficiency of open-type track tank microalgae cultivation is substantially improved, microalgae growth rate and cultivating density are substantially improved, cultivating period is reduced, and apparatus cost and operation cost of scaled microalgae cultivation are reduced.
Owner:QINGDAO INST OF BIOENERGY & BIOPROCESS TECH CHINESE ACADEMY OF SCI
Novel high pH induction and carbon dioxide emission reduction coupling microalgae harvesting method
ActiveCN103013833AEfficient ConcentrationEfficient cost recoveryUnicellular algaeMicroorganism based processesBiologyCarbon dioxide
The invention discloses a novel high pH induction and carbon dioxide emission reduction coupling microalgae harvesting method. Microalgae cells are self-settled and efficiently concentrated by using high pH induction so that high-efficiency and low-cost harvesting is realized. A CO2 emission reduction carbon replenishing technology is coupled, acidic gas CO2 is efficiently absorbed by using harvested high-alkali supernate to ensure that the pH of the high-alkali supernate is reduced to be a level (pH>=8.2) suitable for microalgae growth, high-efficiency carbon replenishing of a culture medium is realized, the supernate after havesting can be recycled, and the harvesting cost and fertilizer cost during large-scale cultivation of the microalgae are greatly lowered.
Owner:SOUTH CHINA SEA INST OF OCEANOLOGY - CHINESE ACAD OF SCI
Light bioreactor for culturing microalgae systematically
ActiveCN101654653ARelieve pressureAvoid breakingBioreactor/fermenter combinationsBiological substance pretreatmentsBiodieselSprayer
The invention relates to a light bioreactor for culturing microalgae systematically, which comprises an in-parallel pipe group, a control valve, a pH value sensor, a temperature sensor, a light sensor, a water mist sprayer, a liquid level alarm, a pipe pump, an air inlet pipe, an exhaust pipe, a feed pipe, a discharge pipe, a microporous pipe, a liquid storage tank, a cool and heat exchanger, a control panel, a bracket, a lighting facility and the like, wherein the pipe group and the valve are effectively connected in a close way, specific surface area is enlarged simultaneously when the volume of microalgae is enlarged during culturing, and the invention has small ground occupation, effectively uses stereo space and has sufficient air exchange, and utilization rate is increased; during culturing, the invention monitors parameters such as temperature, pH value, concentration of a culture medium, lighting intensity, and the like in real time, provides the best condition for microalgae growth and metabolism, effectively reduces culturing time, avoids danger of outside harmful creatures, and decreases production cost. The bioreactor can be used for culturing microalgae cells in a continuous or semi-continuous way and producing microalgae protein, biodiesel or aquatic baits and other products.
Owner:云南爱尔发生物技术股份有限公司
Method for reducing CO2 emission and producing microalgae lipid by using microalgae
InactiveCN102441325AIncrease fix rateLow pollution rateDispersed particle separationFatty-oils/fats productionParticulatesVolume concentration
The invention relates to a method for reducing CO2 emission and producing microalgae lipid by using microalgae. The method comprises the following processing steps of: (1) treating and pressurizing exhaust gas emitted from a factory, removing liquid drops and particles from the CO2-containing exhaust gas emitted from the factory, and pressurizing the CO2-containing exhaust gas to 0.1-2.5 Mpa; (2) adjusting the content of CO2 in the exhaust gas, and adjusting the exhaust gas treated and pressurized in the step (1) so that the volume concentration of CO2 contained in the exhaust gas reaches 10-25%; and (3) fixing CO2 by using microalgae, injecting a microalgae culture medium into a sealed bioreactor, adding microalgae spices in the logarithmic phase into the sealed bioreactor with the inoculation quantity of 5%-20%, introducing the exhaust gas the CO2 concentration of which is adjusted in the step (2) into the sealed bioreactor with the flow rate of 500-1000 ml / min under stirring, and culturing microalgae at a temperature suitable for microalgae growth to fix CO2.
Owner:曹曦跃
Microalgae high-throughout culture equipment
ActiveCN102212471AImprove light uniformityIncrease light intensityBioreactor/fermenter combinationsBiological substance pretreatmentsTemperature controlLight guide
The invention provides microalgae high-throughout culture equipment comprising a frame, an oscillating mechanism, a temperature control device, a humidity control device, a ventilation control device, an objective table and a lighting device, wherein the objective table is suitable for fixing a culturing structure for containing an algae seed or algae liquid, the lighting device is arranged belowthe objective table and is suitable for providing even lighting for microalgae culture, the lighting device consists of a light source and a light guiding structure which comprises a light guiding layer, light reflection structures are arranged on the side surfaces and bottom surface of the light guiding layer so as to guide light from the light source to emit out from one surface of the light guide layer, therefore, light from the light source is evenly provided to the culturing structure containing the algae seed or algae liquid, and the lighting uniformity degree and lighting intensity formicroalgae high-throughout culture can be improved so as to meet the demands of microalgae growth and high-throughout screening.
Owner:ENN SCI & TECH DEV +2
Culture method of microalgae
ActiveCN103773687AQuality impactAvoid killingUnicellular algaeMicroorganism based processesAntibiotic YAutotrophic Growth
The invention discloses a culture method of microalgae. The method comprises the following steps: (1) conducting domestication and culture on an original seed liquid of microalgae in autotrophic growth conditions by using a mode of increasing the pH value step by step, wherein the initial pH value is 8.0-9.0, which is increased by 2-8 times with pH increasing by 0.5-2 every time; and culturing the microalgae under the same conditions for 24-96 h after each time of pH value increasing, and domesticating and culturing to reach a pH value of 11.0-12.0, so as to obtain a microalgae liquid with high pH tolerance; (2) inoculating the domesticated microalgae liquid to a heterotrophic medium with pH value of 11.0-12.0 and cultivating for 10-24 h, then adjusting the pH vale of the culture medium to 7.0-8.0 and carrying out further culture; and (3) when the microalgae growth achieves a stable period, stopping the cultivation and harvesting microalgae cells. The method can effectively inhibit the growth of infectious microbes in microalgae culture, does not require addition of antibiotics, does not have adverse influences on the quality of microalgae, and is safe, reliable and low in price.
Owner:CHINA PETROLEUM & CHEM CORP +1
Novel method for culturing microalgae
InactiveCN103382443AEasy to separate and harvestSolve the shortage of supplyUnicellular algaeWater savingInternal pressure
The invention belongs to the field of microalgae culture and discloses a novel method for culturing microalgae. The novel method is characterized in that in an enclosed environment (1), a net having a certain area and a certain aperture size is used as an attachment (3) for growth of microalgae; multiple film plates are arranged horizontally from top to bottom; a distance is formed between every two adjacent film plates so that enough light is guaranteed; a culture solution is uniformly added on the film plates by a spraying system (4) and is used for microalgae growth; a carbon dioxide supply system (2) can supply enough carbon dioxide to the whole system so that normal microalgae photosynthesis can be guaranteed; and a gas adjustment system (5) guarantees normal system internal pressure and smooth supplement of carbon dioxide. Compared with the existing microalgae culture technology, the novel method has the advantages that microalgae can be separated and harvested easily; the problem of insufficient carbon dioxide supplement of the traditional culture mode is solved; water is saved; operation is simple; and a cost is low.
Owner:GUANGXI ACAD OF SCI
Microalgae Growth Pond Design
InactiveUS20110287531A1Ensure productivityBioreactor/fermenter combinationsBiological substance pretreatmentsEngineeringMicroalgae growth
A raceway pond for circulating microalgae in a fluid medium includes a plurality of interconnected channels. Each channel is straight and has a structured gradient, due to tilt or terracing, that moves the fluid medium along the raceway. In operation, the concentration of microalgae in the fluid medium is maintained substantially constant, and the depth of the fluid medium in the raceway is maintained below a pre-determined level.
Owner:GENERAL ATOMICS
Method for microalgae breeding and biological oil co-production
InactiveCN103215188AResolve cases that depend on external joinsImprove growth efficiencyUnicellular algaeFermentationInorganic saltsNutrient solution
The present invention relates to a novel method for microalgae circulation breeding and biological oil co-production, wherein additional nutrition substances are not required to be added, and the method can be provided for low cost microalgae culture and biological oil co-production. The method mainly comprises a novel microalgae circulation breeding manner and application of the circulation breeding process to co-produce the biological oil, wherein the microalgae collected from each generation breeding is subjected to hydrolysis to obtain a water phase and an oil phase, the water phase is adopted as a microalgae circulation breeding nutrition liquid to be added to a microalgae breeding system so as to provide a carbon source, a nitrogen source, a phosphorus source and inorganic salts, and achieve a purpose of circulation breeding, and fatty acids contained in the oil phase are the products. The method for microalgae breeding has characteristics of high efficiency, rapid microalgae growth, no requirement of additional nitrogen source, phosphorus source and inorganic salts, biological oil co-production, high economic values and high industrial application prospects.
Owner:DALIAN INST OF CHEM PHYSICS CHINESE ACAD OF SCI
Built-in light source bioreactor and microalgae culture method
InactiveCN104017726APromote growthRealize three-dimensional farmingBioreactor/fermenter combinationsBiological substance pretreatmentsControllabilityDistributor
The invention discloses a built-in light source bioreactor. The built-in light source bioreactor comprises a reaction vessel, a material feed and discharge device, a light-emitting device, a nutrient distributing device and a gas distributor, wherein the reaction vessel is provided with a cover plate, and used for containing a culture solution for microalgae growth; the material feed and discharge device is connected with the reaction vessel in a seal mode and provided with a valve and a switch, and the microalgae are pumped in or out of the reaction vessel through the material feed and discharge device; the light-emitting device is used for generating a light source required by microalgae growth; the nutrient distributing device is used for supplying nutrients by microalgae growth into the reaction vessel; and the gas distributor is used for supplying gas required by microalgae growth into the reaction vessel. The built-in light source bioreactor is free from the influence of weather changes, can perform the microalgae reproduction by different stages, has high controllability, and is beneficial to implementing stable and continuous industrial production; and the bioreactor tandem and / or parallel system enhances the large-scale culture efficiency of microalgae.
Owner:LIVINGZONE SHANGHAI BIO CHEM TECH CO LTD
Photobioreactor for culturing microalgae using hollow fiber membrane
InactiveUS20120288928A1Promote growthMaximizing carbon dioxideBioreactor/fermenter combinationsBiological substance pretreatmentsPhotobioreactorCirculating pump
Disclosed is a high-speed photobioreactor for culturing microalgae using a hollow fiber membrane. More particularly a high-speed photobioreactor for culturing microalgae using a hollow fiber membrane capable of facilitating growth of microalgae and maximizing carbon dioxide fixation by increasing the rate of carbon dioxide saturation in a culture medium. Specifically, a high-speed photobioreactor for culturing microalgae using a hollow fiber membrane includes a reactor main body for culturing microalgae; a hollow fiber membrane module for supplying carbon dioxide into a culture medium in the reactor main body; a culture medium circulation pump for circulating the culture medium; and a defoamer for removing foams produced in the culture medium.
Owner:HYUNDAI MOTOR CO LTD +1
Device capable of producing hydrogen peroxide and application of device
ActiveCN109628272AAchieve productionReduce energy consumptionCellsBioreactor/fermenter combinationsCathode reactionEnergy consumption
The invention discloses a device capable of producing hydrogen peroxide and application of the device, and belongs to the technical field of oxidants. According to the device, O2 can be provided for cathode reaction by using microalgae photosynthesis to achieve the production of the hydrogen peroxide, external aeration is not needed, the energy consumption is reduced, and microalgae production canbe achieved by the device. By producing the hydrogen peroxide for 8 days by using the device, the oil yield can be allowed to reach up to 656 mg / L d in a microalgae growing chamber, the accumulationconcentration of H2O2 in a cathode chamber is 180 mg / L, the effect is excellent, and the application prospects are wide.
Owner:JIANGNAN UNIV
Method for improving oil content of oil-producing microalgae based on fulvic acid
InactiveCN105296553APromote growthPromote accumulationBiofuelsMicroorganism based processesOil and greaseBiodiesel
The invention discloses a method for improving oil content of oil-producing microalgae based on fulvic acid. The method comprises the steps of preparing a culture medium based on fulvic acid; placing oil-producing monoraphidium in the culture medium to perform shaking culture; and preparing biodiesel. The fulvic acid improves microalgae growth rate and oil content, and the method has the advantages of being low in investment, simple to operate and high in efficiency. The method is favorable for urging the microalgae to absorb more nutrient more quickly and promoting development and growth of the microalgae. The activity of multiple synthetase is improved, the culture time of the microalgae is shortened, the oil content is greatly improved, and the method is of great significance to microalgae biodiesel production industrialization.
Owner:KUNMING UNIV OF SCI & TECH
Freshwater eukaryoticmicroalgae culture method
InactiveCN105316235AMeet the needs of industrializationUnicellular algaeEnzymology/microbiology apparatusFresh water organismBiology
The invention discloses a freshwater eukaryoticmicroalgae culture method. The method includes steps: (1), providing culture equipment used for culturing freshwater eukaryoticmicroalgae, wherein the culture equipment comprises a reaction container, a light emitting device, a nutrition distributing device and a gas supply device, the reaction container is used for containing a culture solution for microalgae growth, the light emitting device provides light needed for microalgae growth in the reaction container, the nutrition distributing device is used for providing nutrients needed for microalgae growth and reproduction and can provide manganese ions for the culture solution, and the gas supply device is used for providing gas needed for microalgae growth in the reaction container; (2), culturing the freshwater eukaryotic microalgae in the culture equipment. An infrared light source is adopted in the method, and a proper amount of manganese ions are provided into and a proper amount of carbon dioxide is fed into the culture solution, so that microalgae culture efficiency is improved greatly.
Owner:SHANGHAI XIMING BIOTECH CO LTD
Method for producing grease rich in essential fatty acid linoleic acid and alpha-linolenic acid through culturing microalgae by use of CO2
InactiveCN102329826ALow costHigh yieldMicroorganism based processesFermentationBiomassScreening cultures
The invention provides a method for producing grease rich in essential fatty acid linoleic acid and alpha-linolenic acid through culturing microalgae by use of CO2. The method comprises the following steps: (1) screening and domesticating pollution-resisting algal species by utilizing a special screening culture system and a special culture medium under the high-efficiency high-CO2-concentration-resisting high-temperature-resisting conditions; (2) culturing domesticated microalgae by use of CO2; and (3) extracting the grease rich in linoleic acid and alpha-linolenic acid by taking the microalgae as a raw material. According to the invention, CO2 emitted by waste gas in a power plant is converted into a substance which can absorbed for growth of microalgae, so that algae cells with high biomass are obtained and high-value essential fatty acid linoleic acid and alpha-linolenic acid grease can be produced; and by using the method, the emission of CO2 is reduced, the greenhouse effect is alleviated, CO2 can be converted into high-value-added linoleic acid and alpha-linolenic acid grease, high yield of grease can be obtained, and the contents of linoleic acid and alpha-linolenic acid are maintained above 60%, thereby ensuring the quality of essential fatty acid grease and reducing the cost for producing the essential fatty acid grease.
Owner:SHANGHAI JIAO TONG UNIV
Method and apparatus for producing lipids through microalgae culture
InactiveCN103627620AIncrease growth rateHigh lipid concentrationBioreactor/fermenter combinationsBiological substance pretreatmentsControl systemSmall footprint
A purpose of the present invention is to provide an apparatus for producing lipids through microalgae culture. The apparatus comprises: at least a photoautotrophy reactor, wherein the photoautotrophy reactor is positioned on the downstream of a tank, is communicated with the tank, and is provided for photoautotrophy growth of microalgae; at least a photoheterotrophy reactor, wherein the photoheterotrophy reactor is positioned on the downstream of the photoautotrophy reactor, is communicated with the photoautotrophy reactor, and is provided for photoheterotrophy growth of microalgae and accumulation of lipids; and a microalgae concentration control system, wherein the microalgae concentration control system controls a dilution rate of the photoautotrophy reactor through monitoring the microalgae concentration in the photoheterotrophy reactor. The present invention further provides a corresponding method. With the apparatus and the method, high microalgae growth speed and high lipid concentration can be achieved, the occupation area is small, the capital investment is low, and the operation cost is low.
Owner:SIEMENS AG
Dunaliella tertiolecta mutant strain with high growth rate and complex mutation breeding method thereof
ActiveCN101597571AIncrease growth rateFast growth rateUnicellular algaeMutant preparationDunaliella tertiolectaScreening cultures
The invention relates to a method adopting ultraviolet mutation and high temperature and light to perform complex mutation on Dunaliella tertiolecta, which performs screening by using the growth rate of microalgae as an index under a normal culture condition to finally obtain a mutagenic strain ENN0001-1 with higher growth rate compared with a wild strain. After re-screening culture, the biomass (OD750) of the mutagenic strain is increased by 17.6 percent compared with the wild strain, and the chlorophyll content (OD680) is increased by 8 percent compared with the wild strain.
Owner:ENN SCI & TECH DEV
High-density rotifer culture device and method
ActiveCN111248139AHigh densityImprove standardsBioreactor/fermenter combinationsBiological substance pretreatmentsFisheryZoology
The invention discloses a high-density rotifer culture device and method. The rotifer breeding device comprises a breeding trough, a microalgae culture barrel and a central control system. The methodcomprises the steps of pre-treatment of the rotifer culture device, microalgae culture, rotifer culture, rotifer collection and cyclic breeding; and through the central control system, the microalgaeculture barrel is controlled, carbon dioxide is continuously supplied to accelerate growth of microalgae, the growth requirements of rotifers are met by controlling the microalgae culture barrel to transport high-density microalgae, as feed, to the breeding trough, and by filling the breeding trough with pure oxygen, and after the rotifers grow to certain density, the central control system conducts pollution discharge and rotifer collection on the breeding trough, and recycles a rotifer culture solution. Through the culture device with dissolved oxygen and high-density food in water increasedand cyclic supply achieved, high-standard high-density rotifer culture is achieved, and the survival rate and the breeding efficiency of rotifer breeding are improved.
Owner:ZHEJIANG MARICULTURE RES INST
Device and method for testing carbon dioxide discharge-reduction
ActiveCN101619286AEmission reductionFlexible mobilityBioreactor/fermenter combinationsBiological substance pretreatmentsWastewaterMaterial resources
The invention discloses a device and a method for testing carbon dioxide discharge-reduction. The device for testing carbon dioxide discharge-reduction comprises a vehicle body, a supply chamber, a microalgae biologic photosynthetic reaction chamber and microalgae growing process testing equipment, wherein the supply chamber is arranged in the vehicle body and is used for supplying waste gas and waste water; the supply chamber is used for supplying water required by the growth of microalgae and the mixed gas of the waste gas and air to the microalgae biologic photosynthetic reaction chamber; the microalgae biologic photosynthetic reaction chamber is provided with a plurality of optical reactors and is used for using the water and the mixed gas provided by the supply chamber and the microalgae placed in each optical reactor to carry out a microalgae growth experiment; and the microalgae growing process testing equipment detects the relevant parameters of the growth quality of the microalgae in each optical reactor of the microalgae biologic photosynthetic reaction chamber so as to determine the microalgae suitable for absorbing the waste gas. The device and the method for testing carbon dioxide discharge-reduction have flexible fluidity, can realize the test on site where the waste gas is generated, enhance the testing efficiency and save manpower and material resources required by the transportation of the waste gas.
Owner:ENN SCI & TECH DEV
Method for processing soy sauce wastewater through microalgae
InactiveCN109626584AReduce the cost of trainingRealize recyclingBacteriaUnicellular algaeProcessing costNutrients substances
A method for processing soy sauce wastewater through microalgae specifically includes the steps of obtaining salt-resistant and stain-resistant microalgae through screening, adding the microalgae to aculture medium for high-density culturing, inoculating harvested microalgae cells into the soy sauce wastewater, and conducting oscillation culturing under the illumination conditions. Since a largeamount of organic matter, nitrogen, phosphorus and other substances in the wastewater can be consumed in the microalgae growth and metabolism process, the concentration of pollutants in the wastewateris reduced, and the aim of purifying the wastewater is realized. The method is simple in operation and small in device input, greatly reduces the wastewater processing cost, meets the microalgae industrial wastewater processing application requirements, and has the advantages of being high in efficiency, environmentally friendly, high in comprehensive utilization rate and the like. The microalgaeefficiently absorb the organic matter, nitrogen, phosphorus and other nutrient substances in the soy sauce wastewater and convert the organic matter, nitrogen, phosphorus and other nutrient substances into high-additional-value biomass, the harvested microalgae cells can be used for subsequent biological energy preparing, feed and bait applying and the like, economic benefits are improved, and resource recycling is sufficiently realized.
Owner:NANCHANG UNIV
Ultrasonic reinforcement culture method of microalgae
InactiveCN104894102APromote growthTo promote metabolismUnicellular algaeMicroorganism based processesLiquid mediumMetabolite
The invention provides an ultrasonic reinforcement culture method of microalgae. The method includes selection and cleaning of a certain amount of algae, production of a microalgae solid medium, and conservation and spread culture of microalgae species in laboratory. The method is as below: first selecting a liquid medium appropriate for microalgae growth, and adding gel to obtain a solid medium; selecting sterile purified microalgae species, inoculating into the solid medium, sealing and culturing; and finally conducting initial expansion culture and relaying culture of microalgae species; and carrying out quality control on microalgae species, and screening out a high-quality microalgae species. Through ultrasonic reinforcement on high-quality microalgae species, cell wall partial rupture caused by ultrasonic improves the permeability of cell membrane, thus promoting the release of metabolites, promoting cell growth and metabolism, and increasing growth rate.
Owner:邹宁
Method for culturing microalgae by using alcohol wastewater and fermentation exhaust
InactiveCN104556566AEfficient degradationMultistage water/sewage treatmentBiological water/sewage treatmentAlcohol productionBottle neck
The invention provides a method for culturing microalgae by using alcohol wastewater and fermentation exhaust, which comprises the steps of anaerobic digestion of alcohol wastewater, microalgae culture, wastewater recovered mixture for alcohol fermentation, and the like. The method specifically comprises the following steps: carrying out anaerobic digestion on the alcohol wastewater; culturing the microalgae by using the liquid obtained by solid-liquid separation to obtain a microalgae thallus product or extracted biological grease, wherein fermentation exhaust is introduced to supplement the carbon source required by microalgae growth in the process; and recovering the microalgae purification liquid to the alcohol production mixture technique. The method for culturing microalgae by using the alcohol anaerobic wastewater and fermentation exhaust can eliminate the inhibiting action of ammonia nitrogen on alcohol fermentation, enhances the feasibility of alcohol fermentation by recovering treated wastewater, implements the zero discharge of the wastewater and fermentation exhaust in the alcohol production process, solves the key technical bottle neck for restricting the clean production of alcohol fermentation, and obtains the high-value microalgae thallus product or biological grease.
Owner:河南天冠生物燃料工程技术有限公司
Method for promoting microalgae growth and grease accumulation in BG-11 culture medium
The invention discloses a method for promoting microalgae growth and grease accumulation in a BG-11 culture medium, and belongs to the technical field of production of biodiesel through microalgae. According to the method, the BG-11 culture medium serves as a basic culture medium for microalgae culture, then a walnut shell extract solution culture medium is prepared, the pH is adjusted to 6.8-7.0,and the two culture mediums are sterilized at 121 DEG C for 20 minutes separately and then mixed and loaded into a photobioreactor. Oil-producing microalgae is inoculated for culture, after the microalgae grows to a stable period, algae cells are collected by centrifugation, and a chloroform / methanol (2:1, v / v) solution is utilized for extracting grease in the algae cells. The method is easy andfeasible to operate, nutrients in walnut shells can be effectively utilized for increasing the biomass and grease content of the microalgae, the production cost is reduced, and the generation of greenhouse gas is reduced.
Owner:KUNMING UNIV OF SCI & TECH +1
Method for purifying undiluted anaerobic fermentation tail liquid by growing microalgae in carbon dioxide environment
InactiveCN105400697AEfficient purificationShortened growth lagUnicellular algaeTreatment involving filtrationHigh concentrationWastewater
The invention relates to purification of organic pollutants in wastewater, and provides a method for purifying undiluted anaerobic fermentation tail liquid by growing microalgae in a carbon dioxide environment. The method comprises the steps of centrifuging the anaerobic fermentation tail liquid, taking supernate as the tail liquid raw material, sterilizing the tail liquid raw material, regulating the pH value of the tail liquid raw material, and then conducting alga species inoculation; controlling temperature and illumination, and introducing carbon dioxide to the inoculated tail liquid raw material for 7-14 days of culture; obtaining microalgae biomass through centrifugal separation after microalgae culture is ended, so that purification of the anaerobic fermentation tail liquid is achieved. Optimal regulation is achieved by growing microalgae in the high-concentration carbon dioxide environment, anaerobic fermentation tail liquid purification and microalgae growth are combined, and organic pollutants in the undiluted pig manure anaerobic fermentation tail liquid can be effectively purified.
Owner:ZHEJIANG UNIV
Microalgae growth pond design
InactiveCN103096708AGuaranteed productivityBioreactor/fermenter combinationsBiological substance pretreatmentsEngineeringMicroalgae growth
Owner:GENERAL ATOMICS
Bioerosion action method of quantitative microalgae to limestone
ActiveCN103173520AGet bioerodive effectMature technologyMicrobiological testing/measurementSoil scienceEnvironmental engineering
Owner:INST OF GEOCHEM CHINESE ACADEMY OF SCI
Pipe and pond combined type gleaming bioreactor system and microalgae growth carbon consolidating method
PendingCN111704990APromote growthImprove light areaBioreactor/fermenter combinationsBiological substance pretreatmentsPulp and paper industryEnvironmental engineering
The invention relates to a biomass energy utilization technique, and aims to provide a pipe and pond combined type gleaming bioreactor system and a microalgae growth carbon consolidating method. The system comprises racetrack pond reactors for culturing microalgae, wherein a plurality of horizontal transverse pipes made from a translucent material are arranged above the corresponding racetrack pond reactor in parallel, and are mutually perpendicular to the length direction of the corresponding racetrack pond reactor; the ends of the adjacent horizontal transverse pipes are connected through abent pipe to form a perforated horizontal transverse pipe reactor; an outlet end of the horizontal transverse pipe reactor is sequentially connected with the racetrack pond reactors and a CO2 dissolver through a pipeline, and the outlet end of the CO2 dissolver is sequentially connected with a centrifugal pump and an inlet end of the horizontal transverse pipe reactor through a pipeline; and an air distributor is arranged in the CO2 dissolver and is connected to a CO2 air source through a pipeline. The pipe and pond combined type gleaming bioreactor system can improve the distribution of a light region and a dark region of the surfaces of algae liquid in a racetrack pond, the gleaming effect when microalgae cells flow is notably reinforced, the gleaming frequency when the microalgae cellsmove in the racetrack pond is increased, and promotion of growth and carbon consolidation by microalgae photosynthesis is facilitated.
Owner:ZHEJIANG UNIV
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