167 results about "Microalgae growth" patented technology

The invention discloses a microalgae automatic counting method based on digital image processing. Digitalized processing is conducted on a preprocessed picture through a matlab image processing method to extract a binary image, morphological processing is conducted on the image, and the amount of microalgae is calculated. The problems of heavy labor and measurement errors caused by manual counting of microalgae microscopic images at present are solved. Meanwhile, the reliable counting method and the reliable counting steps are provided for the difficult problem that due to the fact that the microalgae microscopic images are easily affected by illumination impurity interference, light-background dark objects and cytoadherence, the microalgae can not be accurately counted, and the microalgae automatic counting method has important significance in microalgae growth monitoring.

The invention discloses a novel high pH induction and carbon dioxide emission reduction coupling microalgae harvesting method. Microalgae cells are self-settled and efficiently concentrated by using high pH induction so that high-efficiency and low-cost harvesting is realized. A CO2 emission reduction carbon replenishing technology is coupled, acidic gas CO2 is efficiently absorbed by using harvested high-alkali supernate to ensure that the pH of the high-alkali supernate is reduced to be a level (pH>=8.2) suitable for microalgae growth, high-efficiency carbon replenishing of a culture medium is realized, the supernate after havesting can be recycled, and the harvesting cost and fertilizer cost during large-scale cultivation of the microalgae are greatly lowered.

The invention relates to a light bioreactor for culturing microalgae systematically, which comprises an in-parallel pipe group, a control valve, a pH value sensor, a temperature sensor, a light sensor, a water mist sprayer, a liquid level alarm, a pipe pump, an air inlet pipe, an exhaust pipe, a feed pipe, a discharge pipe, a microporous pipe, a liquid storage tank, a cool and heat exchanger, a control panel, a bracket, a lighting facility and the like, wherein the pipe group and the valve are effectively connected in a close way, specific surface area is enlarged simultaneously when the volume of microalgae is enlarged during culturing, and the invention has small ground occupation, effectively uses stereo space and has sufficient air exchange, and utilization rate is increased; during culturing, the invention monitors parameters such as temperature, pH value, concentration of a culture medium, lighting intensity, and the like in real time, provides the best condition for microalgae growth and metabolism, effectively reduces culturing time, avoids danger of outside harmful creatures, and decreases production cost. The bioreactor can be used for culturing microalgae cells in a continuous or semi-continuous way and producing microalgae protein, biodiesel or aquatic baits and other products.

The invention relates to a method for reducing CO2 emission and producing microalgae lipid by using microalgae. The method comprises the following processing steps of: (1) treating and pressurizing exhaust gas emitted from a factory, removing liquid drops and particles from the CO2-containing exhaust gas emitted from the factory, and pressurizing the CO2-containing exhaust gas to 0.1-2.5 Mpa; (2) adjusting the content of CO2 in the exhaust gas, and adjusting the exhaust gas treated and pressurized in the step (1) so that the volume concentration of CO2 contained in the exhaust gas reaches 10-25%; and (3) fixing CO2 by using microalgae, injecting a microalgae culture medium into a sealed bioreactor, adding microalgae spices in the logarithmic phase into the sealed bioreactor with the inoculation quantity of 5%-20%, introducing the exhaust gas the CO2 concentration of which is adjusted in the step (2) into the sealed bioreactor with the flow rate of 500-1000 ml/min under stirring, and culturing microalgae at a temperature suitable for microalgae growth to fix CO2.

The invention provides microalgae high-throughout culture equipment comprising a frame, an oscillating mechanism, a temperature control device, a humidity control device, a ventilation control device, an objective table and a lighting device, wherein the objective table is suitable for fixing a culturing structure for containing an algae seed or algae liquid, the lighting device is arranged belowthe objective table and is suitable for providing even lighting for microalgae culture, the lighting device consists of a light source and a light guiding structure which comprises a light guiding layer, light reflection structures are arranged on the side surfaces and bottom surface of the light guiding layer so as to guide light from the light source to emit out from one surface of the light guide layer, therefore, light from the light source is evenly provided to the culturing structure containing the algae seed or algae liquid, and the lighting uniformity degree and lighting intensity formicroalgae high-throughout culture can be improved so as to meet the demands of microalgae growth and high-throughout screening.

The invention discloses a culture method of microalgae. The method comprises the following steps: (1) conducting domestication and culture on an original seed liquid of microalgae in autotrophic growth conditions by using a mode of increasing the pH value step by step, wherein the initial pH value is 8.0-9.0, which is increased by 2-8 times with pH increasing by 0.5-2 every time; and culturing the microalgae under the same conditions for 24-96 h after each time of pH value increasing, and domesticating and culturing to reach a pH value of 11.0-12.0, so as to obtain a microalgae liquid with high pH tolerance; (2) inoculating the domesticated microalgae liquid to a heterotrophic medium with pH value of 11.0-12.0 and cultivating for 10-24 h, then adjusting the pH vale of the culture medium to 7.0-8.0 and carrying out further culture; and (3) when the microalgae growth achieves a stable period, stopping the cultivation and harvesting microalgae cells. The method can effectively inhibit the growth of infectious microbes in microalgae culture, does not require addition of antibiotics, does not have adverse influences on the quality of microalgae, and is safe, reliable and low in price.

The invention belongs to the field of microalgae culture and discloses a novel method for culturing microalgae. The novel method is characterized in that in an enclosed environment (1), a net having a certain area and a certain aperture size is used as an attachment (3) for growth of microalgae; multiple film plates are arranged horizontally from top to bottom; a distance is formed between every two adjacent film plates so that enough light is guaranteed; a culture solution is uniformly added on the film plates by a spraying system (4) and is used for microalgae growth; a carbon dioxide supply system (2) can supply enough carbon dioxide to the whole system so that normal microalgae photosynthesis can be guaranteed; and a gas adjustment system (5) guarantees normal system internal pressure and smooth supplement of carbon dioxide. Compared with the existing microalgae culture technology, the novel method has the advantages that microalgae can be separated and harvested easily; the problem of insufficient carbon dioxide supplement of the traditional culture mode is solved; water is saved; operation is simple; and a cost is low.

A raceway pond for circulating microalgae in a fluid medium includes a plurality of interconnected channels. Each channel is straight and has a structured gradient, due to tilt or terracing, that moves the fluid medium along the raceway. In operation, the concentration of microalgae in the fluid medium is maintained substantially constant, and the depth of the fluid medium in the raceway is maintained below a pre-determined level.

The invention discloses a built-in light source bioreactor. The built-in light source bioreactor comprises a reaction vessel, a material feed and discharge device, a light-emitting device, a nutrient distributing device and a gas distributor, wherein the reaction vessel is provided with a cover plate, and used for containing a culture solution for microalgae growth; the material feed and discharge device is connected with the reaction vessel in a seal mode and provided with a valve and a switch, and the microalgae are pumped in or out of the reaction vessel through the material feed and discharge device; the light-emitting device is used for generating a light source required by microalgae growth; the nutrient distributing device is used for supplying nutrients by microalgae growth into the reaction vessel; and the gas distributor is used for supplying gas required by microalgae growth into the reaction vessel. The built-in light source bioreactor is free from the influence of weather changes, can perform the microalgae reproduction by different stages, has high controllability, and is beneficial to implementing stable and continuous industrial production; and the bioreactor tandem and/or parallel system enhances the large-scale culture efficiency of microalgae.

The invention discloses a device capable of producing hydrogen peroxide and application of the device, and belongs to the technical field of oxidants. According to the device, O2 can be provided for cathode reaction by using microalgae photosynthesis to achieve the production of the hydrogen peroxide, external aeration is not needed, the energy consumption is reduced, and microalgae production canbe achieved by the device. By producing the hydrogen peroxide for 8 days by using the device, the oil yield can be allowed to reach up to 656 mg/L d in a microalgae growing chamber, the accumulationconcentration of H2O2 in a cathode chamber is 180 mg/L, the effect is excellent, and the application prospects are wide.

The invention discloses a method for improving oil content of oil-producing microalgae based on fulvic acid. The method comprises the steps of preparing a culture medium based on fulvic acid; placing oil-producing monoraphidium in the culture medium to perform shaking culture; and preparing biodiesel. The fulvic acid improves microalgae growth rate and oil content, and the method has the advantages of being low in investment, simple to operate and high in efficiency. The method is favorable for urging the microalgae to absorb more nutrient more quickly and promoting development and growth of the microalgae. The activity of multiple synthetase is improved, the culture time of the microalgae is shortened, the oil content is greatly improved, and the method is of great significance to microalgae biodiesel production industrialization.

The invention discloses a freshwater eukaryoticmicroalgae culture method. The method includes steps: (1), providing culture equipment used for culturing freshwater eukaryoticmicroalgae, wherein the culture equipment comprises a reaction container, a light emitting device, a nutrition distributing device and a gas supply device, the reaction container is used for containing a culture solution for microalgae growth, the light emitting device provides light needed for microalgae growth in the reaction container, the nutrition distributing device is used for providing nutrients needed for microalgae growth and reproduction and can provide manganese ions for the culture solution, and the gas supply device is used for providing gas needed for microalgae growth in the reaction container; (2), culturing the freshwater eukaryotic microalgae in the culture equipment. An infrared light source is adopted in the method, and a proper amount of manganese ions are provided into and a proper amount of carbon dioxide is fed into the culture solution, so that microalgae culture efficiency is improved greatly.

The invention provides a method for producing grease rich in essential fatty acid linoleic acid and alpha-linolenic acid through culturing microalgae by use of CO2. The method comprises the following steps: (1) screening and domesticating pollution-resisting algal species by utilizing a special screening culture system and a special culture medium under the high-efficiency high-CO2-concentration-resisting high-temperature-resisting conditions; (2) culturing domesticated microalgae by use of CO2; and (3) extracting the grease rich in linoleic acid and alpha-linolenic acid by taking the microalgae as a raw material. According to the invention, CO2 emitted by waste gas in a power plant is converted into a substance which can absorbed for growth of microalgae, so that algae cells with high biomass are obtained and high-value essential fatty acid linoleic acid and alpha-linolenic acid grease can be produced; and by using the method, the emission of CO2 is reduced, the greenhouse effect is alleviated, CO2 can be converted into high-value-added linoleic acid and alpha-linolenic acid grease, high yield of grease can be obtained, and the contents of linoleic acid and alpha-linolenic acid are maintained above 60%, thereby ensuring the quality of essential fatty acid grease and reducing the cost for producing the essential fatty acid grease.

A purpose of the present invention is to provide an apparatus for producing lipids through microalgae culture. The apparatus comprises: at least a photoautotrophy reactor, wherein the photoautotrophy reactor is positioned on the downstream of a tank, is communicated with the tank, and is provided for photoautotrophy growth of microalgae; at least a photoheterotrophy reactor, wherein the photoheterotrophy reactor is positioned on the downstream of the photoautotrophy reactor, is communicated with the photoautotrophy reactor, and is provided for photoheterotrophy growth of microalgae and accumulation of lipids; and a microalgae concentration control system, wherein the microalgae concentration control system controls a dilution rate of the photoautotrophy reactor through monitoring the microalgae concentration in the photoheterotrophy reactor. The present invention further provides a corresponding method. With the apparatus and the method, high microalgae growth speed and high lipid concentration can be achieved, the occupation area is small, the capital investment is low, and the operation cost is low.

The invention relates to a method adopting ultraviolet mutation and high temperature and light to perform complex mutation on Dunaliella tertiolecta, which performs screening by using the growth rate of microalgae as an index under a normal culture condition to finally obtain a mutagenic strain ENN0001-1 with higher growth rate compared with a wild strain. After re-screening culture, the biomass (OD750) of the mutagenic strain is increased by 17.6 percent compared with the wild strain, and the chlorophyll content (OD680) is increased by 8 percent compared with the wild strain.

The invention discloses a high-density rotifer culture device and method. The rotifer breeding device comprises a breeding trough, a microalgae culture barrel and a central control system. The methodcomprises the steps of pre-treatment of the rotifer culture device, microalgae culture, rotifer culture, rotifer collection and cyclic breeding; and through the central control system, the microalgaeculture barrel is controlled, carbon dioxide is continuously supplied to accelerate growth of microalgae, the growth requirements of rotifers are met by controlling the microalgae culture barrel to transport high-density microalgae, as feed, to the breeding trough, and by filling the breeding trough with pure oxygen, and after the rotifers grow to certain density, the central control system conducts pollution discharge and rotifer collection on the breeding trough, and recycles a rotifer culture solution. Through the culture device with dissolved oxygen and high-density food in water increasedand cyclic supply achieved, high-standard high-density rotifer culture is achieved, and the survival rate and the breeding efficiency of rotifer breeding are improved.

The invention discloses a device and a method for testing carbon dioxide discharge-reduction. The device for testing carbon dioxide discharge-reduction comprises a vehicle body, a supply chamber, a microalgae biologic photosynthetic reaction chamber and microalgae growing process testing equipment, wherein the supply chamber is arranged in the vehicle body and is used for supplying waste gas and waste water; the supply chamber is used for supplying water required by the growth of microalgae and the mixed gas of the waste gas and air to the microalgae biologic photosynthetic reaction chamber; the microalgae biologic photosynthetic reaction chamber is provided with a plurality of optical reactors and is used for using the water and the mixed gas provided by the supply chamber and the microalgae placed in each optical reactor to carry out a microalgae growth experiment; and the microalgae growing process testing equipment detects the relevant parameters of the growth quality of the microalgae in each optical reactor of the microalgae biologic photosynthetic reaction chamber so as to determine the microalgae suitable for absorbing the waste gas. The device and the method for testing carbon dioxide discharge-reduction have flexible fluidity, can realize the test on site where the waste gas is generated, enhance the testing efficiency and save manpower and material resources required by the transportation of the waste gas.

A method for processing soy sauce wastewater through microalgae specifically includes the steps of obtaining salt-resistant and stain-resistant microalgae through screening, adding the microalgae to aculture medium for high-density culturing, inoculating harvested microalgae cells into the soy sauce wastewater, and conducting oscillation culturing under the illumination conditions. Since a largeamount of organic matter, nitrogen, phosphorus and other substances in the wastewater can be consumed in the microalgae growth and metabolism process, the concentration of pollutants in the wastewateris reduced, and the aim of purifying the wastewater is realized. The method is simple in operation and small in device input, greatly reduces the wastewater processing cost, meets the microalgae industrial wastewater processing application requirements, and has the advantages of being high in efficiency, environmentally friendly, high in comprehensive utilization rate and the like. The microalgaeefficiently absorb the organic matter, nitrogen, phosphorus and other nutrient substances in the soy sauce wastewater and convert the organic matter, nitrogen, phosphorus and other nutrient substances into high-additional-value biomass, the harvested microalgae cells can be used for subsequent biological energy preparing, feed and bait applying and the like, economic benefits are improved, and resource recycling is sufficiently realized.

The invention provides an ultrasonic reinforcement culture method of microalgae. The method includes selection and cleaning of a certain amount of algae, production of a microalgae solid medium, and conservation and spread culture of microalgae species in laboratory. The method is as below: first selecting a liquid medium appropriate for microalgae growth, and adding gel to obtain a solid medium; selecting sterile purified microalgae species, inoculating into the solid medium, sealing and culturing; and finally conducting initial expansion culture and relaying culture of microalgae species; and carrying out quality control on microalgae species, and screening out a high-quality microalgae species. Through ultrasonic reinforcement on high-quality microalgae species, cell wall partial rupture caused by ultrasonic improves the permeability of cell membrane, thus promoting the release of metabolites, promoting cell growth and metabolism, and increasing growth rate.

The invention provides a method for culturing microalgae by using alcohol wastewater and fermentation exhaust, which comprises the steps of anaerobic digestion of alcohol wastewater, microalgae culture, wastewater recovered mixture for alcohol fermentation, and the like. The method specifically comprises the following steps: carrying out anaerobic digestion on the alcohol wastewater; culturing the microalgae by using the liquid obtained by solid-liquid separation to obtain a microalgae thallus product or extracted biological grease, wherein fermentation exhaust is introduced to supplement the carbon source required by microalgae growth in the process; and recovering the microalgae purification liquid to the alcohol production mixture technique. The method for culturing microalgae by using the alcohol anaerobic wastewater and fermentation exhaust can eliminate the inhibiting action of ammonia nitrogen on alcohol fermentation, enhances the feasibility of alcohol fermentation by recovering treated wastewater, implements the zero discharge of the wastewater and fermentation exhaust in the alcohol production process, solves the key technical bottle neck for restricting the clean production of alcohol fermentation, and obtains the high-value microalgae thallus product or biological grease.

The invention relates to a biomass energy utilization technique, and aims to provide a pipe and pond combined type gleaming bioreactor system and a microalgae growth carbon consolidating method. The system comprises racetrack pond reactors for culturing microalgae, wherein a plurality of horizontal transverse pipes made from a translucent material are arranged above the corresponding racetrack pond reactor in parallel, and are mutually perpendicular to the length direction of the corresponding racetrack pond reactor; the ends of the adjacent horizontal transverse pipes are connected through abent pipe to form a perforated horizontal transverse pipe reactor; an outlet end of the horizontal transverse pipe reactor is sequentially connected with the racetrack pond reactors and a CO2 dissolver through a pipeline, and the outlet end of the CO2 dissolver is sequentially connected with a centrifugal pump and an inlet end of the horizontal transverse pipe reactor through a pipeline; and an air distributor is arranged in the CO2 dissolver and is connected to a CO2 air source through a pipeline. The pipe and pond combined type gleaming bioreactor system can improve the distribution of a light region and a dark region of the surfaces of algae liquid in a racetrack pond, the gleaming effect when microalgae cells flow is notably reinforced, the gleaming frequency when the microalgae cellsmove in the racetrack pond is increased, and promotion of growth and carbon consolidation by microalgae photosynthesis is facilitated.