The invention discloses a micro-fluidic
chip for
microalgae growth stress detection and a
microscopic image analysis method, and belongs to the technical field of
cell monitoring, the micro-fluidic
chip comprises an inlet, the right side of the inlet is connected with a
cell leading-in area, the right side of the
cell leading-in area is connected with a cell arrangement area, the cell arrangement area comprises at least one micro-convex flow channel, and the micro-convex flow channel is connected with the micro-convex flow channel. The right port of the micro-convex flow channel is connected with a
cell growth area, the
cell growth area comprises a narrow flow channel and a culture flow channel arranged in the narrow flow channel, the
cell growth area is connected with a discharged liquid collection area, the right end of the discharged liquid collection area is connected with an outlet, and a microscopic camera device is arranged above the culture flow channel. According to the micro-fluidic
chip for detecting the growth stress of the microalgae and the
microscopic image analysis method, a micro-fluidic environment is constructed on the micro-chip, fine regulation and control on the growth environment of the microalgae cells are realized, the form and behavior change of the microalgae cells can be observed in real time and at
high resolution, and the micro-fluidic chip can be used for detecting the growth stress of the microalgae in combination with the
microscopic image and the
analysis method. The detection and the analysis on the microalgae cell growth
extrusion stress are realized.