104 results about "Microalgae growth" patented technology

The invention relates to the technical field of microalgae biological intelligent control, in particular to a microalgae growth prediction and optimization method and system based on machine learning. Based on the characteristic variable random forest model of the microalgae growth parameters, screening out a characteristic variable data set after dimensionality reduction screening which significantly affects the microalgae growth, and performing regression training of potential principal component variables on the characteristic variable data set through a machine learning algorithm to obtain a trained microalgae growth prediction model; acquiring a plurality of actual prediction results under dynamic change of culture conditions by using the trained microalgae growth prediction model, and searching a given parameter regulation space in a genetic algorithm based on the actual prediction results of microalgae growth culture to obtain an optimal culture condition parameter set; and issuing the optimal culture condition parameter set to an actual culture process of microalgae growth. According to the method, microalgae growth dynamics can be accurately described in a multi-dimensional parameter space, and real-time prediction and optimization of the microalgae growth trend and metabolic yield are realized.

The invention relates to a carbon quantum dot for strengthening and promoting growth of microalgae and application thereof. In the process of preparing the carbon quantum dots by a conventional hydrothermal method, a precursor mixture is subjected to acidification modification in advance, the pH value is adjusted to 2 by using nitric acid, and then a hydrothermal reaction is performed, so that the prepared carbon quantum dots have the capability of promoting the growth of microalgae. However, if other acids are adopted or pH adjustment is not in place, the effect cannot be achieved, and even the inhibition effect can be achieved. The obtained product is good in effect and stable in performance, no special reaction device needs to be additionally arranged in the acidification modification process, and the method has the advantages that operation is easy and convenient, raw materials are easy to obtain, and the process can be quantified; the method is simple and convenient in process operation, has universality and is easy for industrial production.

The invention discloses a method for regreening gobi deserts by utilizing microalgae crust and application, and belongs to the technical field of ecological restoration. The method mainly adopts a mode of combining microalgae spraying liquid with an engineering remediation method. When the microalgae spraying liquid is prepared, indigenous microalgae and known domesticated desert algae are mixed and co-cultured to prepare a spraying agent, and then soil bacillus is added before spraying. The engineering regulation method mainly comprises the following steps: forming a concave-convex structure by piling up soil mound bags and digging ditches, increasing the specific surface area of the growth of the microalgae spraying liquid by using the uneven land surface layer, and reducing the evaporation speed of the moisture of the microalgae spraying liquid by using the concave land, thereby promoting the growth rate and adaptability of the microalgae and improving the skinning efficiency. In addition, nutritional functional organic fertilizers and / or nano conditioners need to be added into the soil to be remediated in advance. According to the method, the vegetation coverage rate can be increased to about 60% from the initial nearly zero, the gobi desert ecological environment can be obviously improved, the soil salinity and alkalinity can be obviously reduced, and the soil water retention capacity is enhanced.

The invention relates to a microalgae feature fusion analysis method and system based on deep learning, and belongs to the technical field of microalgae feature recognition and analys.The microalgae collection time node is obtained according to the microalgae growth collection threshold range, and the real-time pollutant data of the current microalgae collection time node are obtained; and finally, pollution analysis is performed according to the real-time pollutant data of the current microalgae harvesting time node, a related regulation and control strategy is generated based on a pollution analysis result, and regulation and control are performed according to the related regulation and control strategy. According to the method, the deep learning network and the generative adversarial network are fused, the cell division laws of the microalgae in different environments and the image features within the preset time can be fused to analyze the microalgae growth data in the target area, and the problem of the pain point of the image technology for microalgae growth and reproduction recognition is solved; the growth stage of the microalgae is accurately grasped, and a theoretical support is provided for identifying, regulating and removing microalgae pollutants in a watershed water body.

Photobioreactors are described herein. The photobioreactors include a vessel for cultivating microalgae. The vessel defines an interior chamber comprising a culture containing medium. The vessel has at least one transparent wall to permit passage of light of a frequency necessary to promote growth of the microalgae in the culture containing medium. The vessel also includes a plurality of sensors, each sensor being configured to monitor at least one environmental condition within the photobioreactor, and at least one flow generator positioned within the vessel. The photobioreactor also includes a controller communicatively coupled to each of the plurality of sensors and the at least one flow generator. The controller is configured to: receive sensor data from each of the plurality of sensors, apply a mathematical model to the sensor data to determine when to adjust at least one environmental condition within the photobioreactor and adjust one or more components of the photobioreactor.

The invention relates to a microalgae photosynthetic carbon sequestration technology, and aims to provide a method for promoting microalgae carbon sequestration by a polyethylene glycol passivated nitrogen-doped carbon dot conversion spectrum. The method comprises the following steps: passivating the surfaces of nitrogen-doped carbon dots by using polyethylene glycol, and then directly adding the passivated nitrogen-doped carbon dots into a microalgae suspension, ultraviolet light with the wavelength of 280-390 nm in external light is converted into blue light with the wavelength of 400-500 nm by passivating the nitrogen-doped carbon dots so as to be directly utilized by microalgae, so that growth and carbon sequestration are promoted while the stress resistance of algae cells is improved. According to the method, damage to algae cells can be greatly reduced by converting an ultraviolet spectrum, and meanwhile, microalgae growth and carbon sequestration can be further promoted by converting ultraviolet light into blue light; the spectrum conversion effect is enhanced, the overall light energy utilization efficiency of microalgae carbon sequestration is improved, light inhibition is relieved, and the stress resistance of microalgae cells is improved, so that microalgae growth carbon sequestration is promoted, and the development of the microalgae carbon sequestration industry is promoted.

The invention discloses a photoconductive enhanced osmunda culture'light tree 'bioreactor for CO2 capture and resource utilization. The bioreactor comprises a transparent tank body made of a transparent material, and refractive fibers and hollow fibers are embedded in the transparent tank body. The refractive fiber is made of a high-light-conductivity material, can introduce and uniformly distribute an external light source into the whole bioreactor, and provides necessary illumination conditions for microalgae. The reactor can effectively capture carbon dioxide and utilize microalgae for resourceful culture. The reactor system provides a suitable microalgae growth environment with sufficient light and sufficient carbon sources for microalgae through gas-liquid separation, and promotes rapid growth of microalgae. The reactor can be applied to the fields of industrial waste gas treatment, greenhouse gas emission reduction, biological energy production, aquaculture wastewater and the like. A CO2 trapping technology is organically combined with a CO2 biosynthesis and resource utilization technology, so that efficient trapping and resource utilization of CO2 are realized.

The invention relates to a device and a method for producing a microalgae liquid fertilizer by utilizing monosodium glutamate wastewater, and belongs to the technical field of microalgae fertilizer production devices and methods. Comprising a dilution device, a buffer pool and a pipeline type photobioreactor which are sequentially communicated through a pipeline, a culture medium inlet and a monosodium glutamate wastewater inlet are formed in the dilution device, and a chlorophyll luminoscope, an ultraviolet visible light detector and a water quality tester are installed on an output pipeline of the pipeline type photobioreactor. And the operation of the buffer pool, the tube (channel) type photobioreactor, the chlorophyll luminoscope, the ultraviolet visible light detector and the water quality tester is controlled by the console. According to the method, a culture medium suitable for microalgae growth is prepared by diluting monosodium glutamate wastewater in a certain proportion and adding necessary nutrient elements, chlorella capable of rapidly proliferating in the monosodium glutamate wastewater is cultured by using a photobioreactor, and the chlorella whole-cell sap can promote wheat seedling growth and rooting through tests.

The invention provides an efficient carbon sequestration microalgae culture pond, and belongs to the technical field of microalgae culture. Comprising a culture pond outer wall and a separation wall fixedly connected to the middle of the culture pond outer wall, a microalgae growth space composed of a first straight channel, a first bent channel, a second straight channel and a second bent channel is formed in the culture pond outer wall through the separation wall, and algae liquid is arranged in the microalgae growth space. When the device is used, the stirring assembly drives algae liquid to flow along the annular water channels of the first straight channel, the first bent channel, the second straight channel and the second bent channel, when the stirring assembly drives the algae liquid to the high position of the spoiler, the gravitational potential energy of the algae liquid is increased, and the algae liquid is converted into algae liquid kinetic energy through the gravitational potential energy in the flowing process; due to the fact that the spoilers are designed to have a certain gradient, when the algae liquid reaches a certain flow velocity, laminar flow is changed into turbulent flow, so that algae cells in the algae liquid are frequently exchanged up and down, the photosynthetic efficiency is enhanced, and absorption of carbon dioxide is promoted.

The present invention discloses a method for promoting the expression of selenoprotein genes in Chlamydomonas reinhardtii and increasing the yield of selenoproteins. S1. Inoculate Chlamydomonas reinhardtii algae with liquid culture medium at 20-30°C and 0-200 μmol / (m 2 S2, taking the Chlamydomonas reinhardtii culture solution, adding sodium selenite and abscisic acid, the abscisic acid concentration in the culture solution is 10-1000 μmol / L, at 20-30°C, 0-200 μmol / (m 2 ·s) Oscillating culture under light conditions. The present invention adds the plant hormone ABA during the selenium-enriched culture of Chlamydomonas reinhardtii, which not only does not affect the growth and biomass accumulation of microalgae, but also promotes the expression of selenoproteins. It is applied in the production of Chlamydomonas reinhardtii algae powder rich in selenoproteins, and can greatly increase the yield of selenoproteins.

The invention discloses a method for reducing pollution and carbon in rare earth ammonia-nitrogen wastewater by utilizing a microalgae closed reaction. The method comprises the following steps: preparing an ammonia-nitrogen wastewater culture medium, inoculating microalgae, pressurizing CO2 in a reactor, regulating and controlling culture and monitoring and harvesting. According to the invention, the waste gas CO2 discharged in the rare earth production process can be effectively utilized, a cheap carbon source is provided for an ammonia nitrogen biological removal system, meanwhile, carbon reduction and decontamination are realized, and the defect that in the current technical scheme, the biological removal of ammonia nitrogen needs to additionally provide commercial carbon nutrition for the system is overcome; the reactor runs under the micro-positive pressure condition, CO2 gas mass transfer is enhanced, a sufficient carbon source is provided for microalgae growth, system escape of ammonia nitrogen and CO2 is avoided, and the defects that in the current technical scheme, the ammonia nitrogen recycling effect is poor, and ammonia nitrogen escapes are overcome.

The present application relates to the technical field of microalgae carbon fixation, and specifically discloses a microalgae environmental adaptation carbon fixation method based on spiral disturbance flow field enhancement, which comprises the following steps: constructing a column type photobioreactor and setting a spiral disturbance plate, selecting high-efficiency carbon fixation Chlorella sp. which is resistant to NO X , SO X , pre-treating and inoculating, precisely regulating environmental parameters such as light and pH, monitoring the carbon fixation process in real time and regulating abnormally, and realizing cyclic operation by adopting semi-continuous culture. The present application enhances gas-liquid mass transfer by spiral disturbance, combines dynamic adaptation and regulation of multiple environmental parameters, improves CO₂ emission reduction rate and microalgae growth efficiency, optimizes reactor mass transfer characteristics, reduces energy consumption, realizes the collaborative treatment of multiple pollutants, and provides standardized technical support for the industrialization of microalgae carbon fixation.

The present invention discloses a device and method for lifting seabed nutrients in a shellfish raft culture area. The shellfish raft includes a raft and a limit cable connected between the seabed and the raft. The device for lifting seabed nutrients in a shellfish raft culture area includes a raft arranged on the seabed, a plurality of groups of jet pipes are fixedly arranged on the raft, and a plurality of jet nozzles are opened on each group of jet pipes. The jet pipes are connected with an air duct, and an upper pressurizing mechanism and a lower pressurizing mechanism are connected to the air duct. The present invention uses the upper pressurizing mechanism and the lower pressurizing mechanism to provide power for the air duct to introduce gas above the sea surface into the seabed, forming an air curtain, so that an upwelling is generated in the raft culture area, thereby lifting the bottom water with a high nutrient salt content to the surface layer, providing bait microalgae for growth, improving the abundance of bait microalgae in the culture area, improving the primary productivity of the raft area, and thus improving the survival rate and yield of raft cultured shellfish. At the same time, it can increase the dissolved oxygen in the surface water, thereby playing a role in optimizing the seawater environment in the culture area.

To provide a microalgae growth promoter.SOLUTION: A microalgae growth promoter contains at least one compound selected from (1) oxo fatty acid or a derivative thereof or a salt thereof or (2) a hydroxylated fatty acid or a derivative thereof or a salt thereof.SELECTED DRAWING: None

The utility model relates to the technical field of microalgae culture, in particular to a microalgae culture device. The microalgae culture device comprises a culture frame and a control box, the culture frame is provided with a plurality of culture layers, and each culture layer is provided with a culture assembly. Each culture layer is provided with a light source assembly, and the light source assemblies are located above the culture assemblies and electrically connected with the control box. An algae liquid detection part is arranged in the culture assembly, is connected with the control box and is used for detecting the microalgae growth state and the light environment in the culture assembly. According to the microalgae culture device provided by the utility model, the light source assembly can output optimal growth illumination for culturing microalgae, so that different illumination requirements of the microalgae in each growth stage are met, and the growth of the microalgae is promoted. Meanwhile, the microalgae culture process is controlled by the control box according to detection data of the microalgae liquid detection piece, manual intervention is not needed, and the reliability and stability of illumination adjustment are effectively improved.

The present invention discloses an efficient microalgae cultivation device, belonging to the fields of solar energy utilization and biotechnology. The microalgae cultivation device includes: a culture container filled with microalgae liquid; a light condensing element, the lower part of the light condensing element is immersed in the microalgae liquid, and the light condensing element is used to adjust the sunlight incident on the light condensing element into parallel light or divergent light and then downwardly introduce it into the microalgae liquid to increase the light intensity in the microalgae liquid; and an orientation adjusting member, the orientation adjusting member is used to control the direction of the sunlight incident on the light condensing element. In this application, the sunlight incident on the light condensing element is adjusted into parallel light or divergent light by the light condensing element and then downwardly introduced into the microalgae liquid, increasing the light intensity in the microalgae liquid, ensuring the light source required for microalgae growth, and being not affected by the microalgae density or the depth of the algae liquid at the same time. This application only uses solar energy without the need for electric energy, reducing the cost of microalgae cultivation.

The application discloses a kind of microalgae-tidal flow subsurface wetland enhanced water body pollutant purification device and method, comprising the following steps: when treating contaminated water, contaminated water is introduced from the top of microalgae growth area, when flowing through microalgae growth area, microalgae assimilates and absorbs pollutants, and provides oxygen-rich environment;When the contaminated water flows through the wetland substrate filling area, the fresh high-activity microalgae carried in the water flow and the bacteria form algal-bacterial symbiotic film, and the contaminated water is intensively purified;The purified water flows out from the water outlet;During the purification process of the contaminated water, the water flow velocity of the microalgae growth area is periodically adjusted, and the attached microalgae is periodically flushed, so that it enters the wetland substrate with water flow to replenish microalgae.

This invention discloses a microalgae cell farm control system, control method, and computer-readable storage medium, including a frame; a photovoltaic power supply module, comprising solar panels made of a semi-transparent material, disposed on the outer periphery of the frame as top and side covers, the solar panels being connected to an energy storage unit located within the frame for storing electrical energy; and a photobioreactor unit disposed within the frame, comprising a reactor body for cultivating microalgae. The beneficial effects of this invention are that by setting multiple semi-transparent solar panels on the outer periphery of the frame, placing the reactor body within the frame, and placing a supplementary lighting unit on one side of the reactor body, the supplementary lighting unit utilizes the electrical energy generated by the solar panels to irradiate the reactor body, supplementing the missing spectrum and providing the necessary light conditions for microalgae growth, thereby improving the utilization rate of light energy and increasing the production capacity of microalgae.

The present application belongs to the field of microalgae biotechnology, microalgae culture regulation and high-value lipid bio-manufacturing, and specifically relates to a method for improving microalgae biomass and eicosapentaenoic acid (EPA) yield by regulating amino acids, which takes microalgae capable of synthesizing EPA as a culture object, adds exogenous amino acids or amino acid combinations to the culture system under non-stress culture conditions containing sufficient inorganic nitrogen source, regulates photosynthesis, growth metabolism and lipid synthesis process of the microalgae, and simultaneously improves microalgae biomass and EPA volumetric yield; by adding specific exogenous amino acids or amino acid combinations under the condition of sufficient nutrition culture, the present application realizes synchronous promotion of microalgae growth and EPA accumulation. Different from traditional methods of relying on stress such as nitrogen deficiency to induce lipid accumulation, the present application can improve microalgae biomass, photosynthetic performance, pigment yield and EPA volumetric yield without significantly inhibiting cell growth and photosynthetic activity.

The invention discloses a microalgae biological culture device and method. The culture device comprises a support and a funnel-shaped round cylinder arranged on the support, a pointed cone is arranged in the center of the funnel-shaped round cylinder, flow guide twisted lines are arranged on the pointed cone, a circulating water injection pipe is arranged on the side wall of the funnel-shaped round cylinder, and a culture water input pipe and a microalgae output pipe are arranged at the bottom of the funnel-shaped round cylinder. The culture method comprises the following steps: pumping culture water into a funnel-shaped cylinder through a culture water input pipe until a target volume is reached, inoculating microalgae, and circularly injecting the culture water into the top of the funnel-shaped cylinder from the bottom of the funnel-shaped cylinder through a circulating water injection pipe to form an inward vortex, so that the microalgae grow; and after the growth is finished, closing the circulating water injection pipe, naturally standing, gathering the microalgae concentrated solution to the central position of the funnel-shaped cylinder, and collecting from the microalgae output pipe. According to the invention, through the funnel-shaped cylinder, the circulating water injection pipe and the flow guide twisted lines, the algae-water mixed liquid forms an inward vortex in the funnel-shaped cylinder, no additional energy waste for stirring is needed, and the cost is reduced.

The invention discloses a culture method of microalgae with high starch content, and belongs to the technical field of new food resource mining. Microalgae are cultured in two stages, the ventilation capacity is fixed in the first stage, the ventilation capacity is dynamically adjusted in the second stage, the ventilation capacity in the early stage of culture is 0.75-1.5 L / min, the ventilation capacity is adjusted to 3-6 L / min when the microalgae start to grow in the exponential stage, and the ventilation capacity is adjusted to 0.75-1.5 L / min when the microalgae start to grow in the later stage of the exponential stage until culture is finished. The high-efficiency culture of the microalgae with high starch content is realized by dynamically adjusting the ventilatory capacity of the second culture stage, so that the microalgae cell powder with high starch content is obtained, and the maximum starch content can reach 61% or above. The method has the advantages of short required culture period, simplicity and convenience, no pollution to the environment in the culture process, and important significance for expanding the microalgae as a new resource food and expanding a new starch resource.

The invention provides an intelligent treatment method for mariculture tail water, and belongs to the technical field of machine learning. By constructing a multi-mode tail water environment-evolution data set, comprehensive perception of a tail water visual image sequence, physicochemical index time sequence data and operation control parameters is realized, and dynamic evolution prediction of a tail water system is carried out based on a virtual environment generation model. Through the steady state identification and mode mining module, the stable operation cluster, the critical boundary and the abrupt change mode of the tail water system can be quantified, control-abrupt change response knowledge is extracted, and a structured basis is provided for optimization decision making. On the basis, a multi-target control optimization model is constructed, efficient regulation and control over the microalgae growth rate, the suspended particle sedimentation rate and the nutritive salt absorption efficiency are achieved, meanwhile, the potential mutation risk is avoided, and steady-state operation of the system is guaranteed. According to the method, intelligentization, precision and multi-objective optimization of the tail water treatment process can be achieved, and efficient and reliable technical support is provided for mariculture tail water ecological management.

The invention discloses a culture medium for realizing large-scale and low-cost growth of microalgae. The culture medium comprises the following raw materials: 0.25-2.5 g / L of water-soluble fertilizer; 25 to 150 mg / L of magnesium sulfate; 10 to 100 mg / L of calcium chloride; 0-80 mg / L of sodium carbonate; and trace elements. According to the culture medium, the production cost of microalgae is reduced, the stability of a formula is improved, and large-scale production in the microalgae industry can be realized, so that the biomass of various microalgae and the accumulation of bioactive substances are improved. Compared with traditional formulas such as BG11, BBM and HUT, the culture cost of the culture medium for producing 1 ton of algae powder is reduced by 30%-60% through accounting. The culture medium provided by the invention has the advantages that the growth speed of the microalgae is increased, the capability of promoting bioactive substances (grease, protein, astaxanthin and polysaccharide) synthesized by the microalgae is improved, and the culture cycle of the microalgae is shortened by 10-35%.

The present invention discloses a method and system for resource utilization of carbon dioxide in wine based on microalgae cultivation. The method comprises the following steps: inoculating microalgae into an algae pond, turning on a lighting device, then introducing the carbon dioxide generated by fermentation in a fermentation tank into the algae pond, and introducing a culture solution into the algae pond. After the microalgae grow for a period of time, the high-density microalgae biomass attached to the packing is filtered through a filter screen, and made into algal fertilizer and returned to the vineyard. The low-concentration algae-containing water is introduced into an artificial wetland and discharged after purification. Through the coupling of the fermentation tank, the algae pond and the artificial wetland, on the one hand, the microalgae are used to fix carbon dioxide to produce algal fertilizer products and return them to the vineyard. On the other hand, the microalgae can also preliminarily treat the wastewater from wine brewing, and the wastewater from microalgae cultivation is deeply treated by the artificial wetland, ultimately achieving low emissions or even zero emissions of the system.

The utility model relates to the field of microalgae cultivation, in particular to a high-precision illumination adjusting device. The high-precision illumination adjusting device comprises lamp beads, the lamp beads have different colors, a lampshade wraps the lamp beads on the first face of a shell, the lampshade is ultrasonically welded to the first face of the shell, a fixing part is fixedly installed on the second face of the shell, and the fixing part is arranged on the second face of the shell. The fixing part is a fixing column with threads, an adjustable buckle structure or a magnetic attraction type adsorption part, the illumination adjusting device further comprises switches and an adjusting knob, each switch controls the multiple lamp beads with the same color, and the adjusting knob controls the brightness of all the lamp beads at the same time. According to the utility model, illumination with proper wavelength and intensity can be provided according to different growth stages of microalgae, and the culture efficiency and quality are improved.

The invention discloses a method for producing high-quality protein raw material microalgae. By combining a reduction product formic acid driven by carbon dioxide clean energy with edible microalgae culture, microalgae growth and protein synthesis are enhanced. The method specifically comprises the following steps: carrying out adaptive evolution of microalgae by using 20-50mM of low-concentration formate, culturing under an illumination condition by using 50-300mM of formate as an auxiliary carbon source, and finally harvesting the microalgae biomass of which the protein content exceeds 30% and the amino acid composition meets the high-quality protein requirements of international grain and agricultural tissues, high-quality protein raw materials of food and feed can be used. The method has a remarkable application prospect in the field of green non-grain high-quality protein raw material production.

The invention discloses a photoelectric microfluidic automatic control method for screening microalgae growth conditions, which is characterized by comprising the following steps: preparing different types of culture medium solutions, respectively introducing the culture medium solutions into a photoelectric wetting chip, and generating different types of culture medium drops on the photoelectric wetting chip; automatically obtaining the current positions and IDs of a plurality of culture medium drops, and automatically obtaining the current positions and IDs of algae drops; according to the current positions and the IDs of the plurality of culture medium drops and the current positions and the IDs of the algae drops, respectively planning moving paths of the plurality of culture medium drops to the corresponding algae drops; respectively driving the plurality of culture medium drops to move to the corresponding algae drops in parallel according to the moving path through the plurality of light spots for fusion; and culturing the fused liquid drops in an illumination incubator so as to screen out a culture medium solution which is most suitable for the growth of the algae drops.

The invention discloses an ecological bait production method based on a food chain, which comprises the following steps: S1, mixing wine brewing enzyme liquid and culture tail water in proportion, and stirring to form a nutrient medium required by microalgae growth; s2, conveying the nutrient medium into a microalgae culture module, and regulating and controlling illumination and dissolved oxygen conditions; part of microalgae is conveyed to the plankton breeding unit to cultivate plankton to construct a live bait chain; s3, tail water generated by breeding flows back to the plankton breeding unit to serve as a nutrition supplement source of plankton, and closed-loop circulation is achieved.

The invention provides a method for regulating and controlling synthesis of microalgae fatty acid by using a long-chain carbon signal substance and application thereof, and the method for regulating and controlling synthesis of microalgae fatty acid by using the long-chain carbon signal substance comprises the following steps: culturing microalgae by using a culture medium to obtain a microalgae culture solution; then, a long-chain carbon signal substance is prepared into a solution, the long-chain carbon signal substance is C14-HSL, and the structural formula of the C14-HSL is shown in the formula I; adding a long-chain carbon signal substance solution into the microalgae culture solution to obtain a mixed solution; and culturing the mixed solution. According to the method, the C14-HSL signal substance is inoculated into the microalgae culture solution, so that the growth of microalgae can be effectively promoted, the relative content and composition of saturated fatty acid, monounsaturated fatty acid and polyunsaturated fatty acid in microalgae cells are influenced, the content of fatty acid related to biodiesel precursor synthesis is increased, and the yield and quality of biodiesel are improved. # imgabs0 #

The invention discloses a micro-fluidic chip for microalgae growth stress detection and a microscopic image analysis method, and belongs to the technical field of cell monitoring, the micro-fluidic chip comprises an inlet, the right side of the inlet is connected with a cell leading-in area, the right side of the cell leading-in area is connected with a cell arrangement area, the cell arrangement area comprises at least one micro-convex flow channel, and the micro-convex flow channel is connected with the micro-convex flow channel. The right port of the micro-convex flow channel is connected with a cell growth area, the cell growth area comprises a narrow flow channel and a culture flow channel arranged in the narrow flow channel, the cell growth area is connected with a discharged liquid collection area, the right end of the discharged liquid collection area is connected with an outlet, and a microscopic camera device is arranged above the culture flow channel. According to the micro-fluidic chip for detecting the growth stress of the microalgae and the microscopic image analysis method, a micro-fluidic environment is constructed on the micro-chip, fine regulation and control on the growth environment of the microalgae cells are realized, the form and behavior change of the microalgae cells can be observed in real time and at high resolution, and the micro-fluidic chip can be used for detecting the growth stress of the microalgae in combination with the microscopic image and the analysis method. The detection and the analysis on the microalgae cell growth extrusion stress are realized.