Method for producing grease rich in essential fatty acid linoleic acid and alpha-linolenic acid through culturing microalgae by use of CO2

A fatty acid linoleic acid, CO2 technology, applied in microorganism-based methods, biochemical equipment and methods, microorganisms, etc., can solve the problems of food crisis, slow growth, low long-term benefits, etc., achieve high oil yield, alleviate greenhouse effect, cost reduction effect

Inactive Publication Date: 2012-01-25
SHANGHAI JIAO TONG UNIV
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

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Problems solved by technology

However, since the basic raw materials of these methods are grain and oil crops, these terrestrial plants have a long growth cycle, slow growth, relatively high requirements on land, and

Method used

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  • Method for producing grease rich in essential fatty acid linoleic acid and alpha-linolenic acid through culturing microalgae by use of CO2
  • Method for producing grease rich in essential fatty acid linoleic acid and alpha-linolenic acid through culturing microalgae by use of CO2
  • Method for producing grease rich in essential fatty acid linoleic acid and alpha-linolenic acid through culturing microalgae by use of CO2

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Experimental program
Comparison scheme
Effect test

Embodiment 1

[0036] (1) Construction of specific screening culture system: buy 250L light incubator, 6 air flow meters, 6 CO 2 flow meter, CO 2 Gas meter, air pump, CO 2 A steel cylinder, a vent tube, a filter membrane, a special gas distribution bracket, and a special vent culture bottle are assembled to form a set that can provide 6 different CO2 at the same time. 2 Concentration of algae screening culture and domestication device.

[0037] (2) Preparation of specific medium: since it is mainly CO 2 Crop carbon source, in order to acclimatize microalgae to CO 2 The preference of the specific medium we configured is mainly to remove the carbon source Na 2 CO 3 BG11 medium. And according to 1mg Na per liter 2 MGEDTA, 36mg CaCl 2 2H 2 O, 75mg MgSO 4 ·7H 2 O, 40 mg K 2 HPO 4 ·3H 2 O, 2.86 mg H 3 BO 3 , 1.81mg MnCl 2 4H 2 O, 0.222mg ZnSO 4 ·7H 2 O, 0.079mg CuSO 4 ·5H 2 O, 0.05mg CoCl 2 ·6H 2 O, 0.391 mg NaMoO 4 2H 2 O and 1500mg NaNO 3 The amount of . to configure a...

Embodiment 2-6

[0042] Test with the similar method of example 1, pass into CO except that the Scenedesmus obliquus after cultivating acclimatization 2 The concentrations were 0.03%, 5%, 10%, 20%, 30%, and 50%, respectively, and other parameters remained unchanged.

[0043] figure 1 Indicated at 5% and 10% concentrations of CO 2 Scenedesmus obliquus had the largest biomass. figure 2 Indicated at 5% and 10% concentrations of CO 2Scenedesmus obliquus for CO 2 the maximum rate of carbon fixation. image 3 Indicated at 50% concentration of CO 2 Scenedesmus obliquus had the highest oil content. Figure 4 Indicated at 0.03%, 5%, 10%, 20%, 30%, 50% CO 2 Under the concentration, the contents of linoleic acid are 13%, 20.65%, 19%, 19.14%, 13.43%, 13.34% respectively; and the contents of α-linolenic acid are 43.35%, 40%, 41.54%, 40.67%, 48.44%, 48.23%, so at concentrations above 5% of CO 2 The total content of essential fatty acids linoleic acid and α-linolenic acid in Scenedesmus obliquus is...

Embodiment 7

[0045] Response surface analysis was used to optimize the culture conditions. The response surface analysis experiment mainly used Minitab 15 software, and the central composite design method (the analysis method in the Minitab software) was adopted to carry out the experimental design. Table 1 refers to the factors and level coding values ​​of the response surface experiment. The independent variables are carbon dioxide concentration and light intensity, and the response variable is biomass. The design and results of the central composite experiment are shown in Table 2. The inoculation density is 0.05g / L; the independent variable light intensity range is 2000lux-10000lux; the independent variable carbon dioxide concentration range is 0.03%-40%; the ventilation flow rate is 0.5vvm; the culture temperature is 30±1°C; 0.1. Figure 5 and Figure 6 Respectively, using the response surface carbon dioxide and light intensity to interact to affect the biomass of Scenedesmus obliqu...

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Abstract

The invention provides a method for producing grease rich in essential fatty acid linoleic acid and alpha-linolenic acid through culturing microalgae by use of CO2. The method comprises the following steps: (1) screening and domesticating pollution-resisting algal species by utilizing a special screening culture system and a special culture medium under the high-efficiency high-CO2-concentration-resisting high-temperature-resisting conditions; (2) culturing domesticated microalgae by use of CO2; and (3) extracting the grease rich in linoleic acid and alpha-linolenic acid by taking the microalgae as a raw material. According to the invention, CO2 emitted by waste gas in a power plant is converted into a substance which can absorbed for growth of microalgae, so that algae cells with high biomass are obtained and high-value essential fatty acid linoleic acid and alpha-linolenic acid grease can be produced; and by using the method, the emission of CO2 is reduced, the greenhouse effect is alleviated, CO2 can be converted into high-value-added linoleic acid and alpha-linolenic acid grease, high yield of grease can be obtained, and the contents of linoleic acid and alpha-linolenic acid are maintained above 60%, thereby ensuring the quality of essential fatty acid grease and reducing the cost for producing the essential fatty acid grease.

Description

technical field [0001] The invention belongs to the field of renewable biological resources and relates to the utilization of CO 2 Method for culturing microalgae to produce oils rich in essential fatty acids linoleic acid and alpha-linolenic acid. Background technique [0002] Global warming caused by excessive emission of greenhouse gases has become one of the focuses of governments, academic circles and business circles. According to statistics from the United Nations Climate Change Agency, the current greenhouse gas is mainly CO 2 , accounting for 76.7%. International concern about climate change has led to the advent of the United Nations Framework Convention on Climate Change (UNFCCC), and the implementation of the "Kyoto Protocol" in 2005 requires countries to undertake greenhouse gases, especially CO. 2 The obligation to reduce emissions has been clearly stipulated. At present, countries such as Europe and the United States have begun to levy CO 2 emission tax, ...

Claims

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Application Information

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IPC IPC(8): C12P7/64C12R1/89
Inventor 缪晓玲唐大海曹和春
Owner SHANGHAI JIAO TONG UNIV
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