65 results about "Screening cultures" patented technology

The invention relates to the technical field of plant tissue culture and plant transgenosis, in particular to a method for co-transformation of an LcSUCLA alpha-LcSUCLA beta-pEGFC binary expression vector and Leymus chinensis double genes. The method comprises the following steps: obtaining leymus chinensis tissue culture sterile calluses, transforming agrobacterium competent by a freeze thawing method, activating agrobacterium tumefaciens, infecting the leymus chinensis calluses and co-culturing, screening and culturing plant transformation materials, differentiating and screening a culture medium and culture conditions, and differentiating and screening a culture medium and culture conditions as well as a rooting culture medium and culture conditions. According to the invention, double-gene co-transformation of leymus chinensis is realized, and a leymus chinensis double-transgenic plant is obtained. The cotransformation of the LcSUCLA alpha and the LcSUCLA beta in the leymus chinensis can synthesize more succinic acid than the single transformation, so that the tolerance of the leymus chinensis under the stress of basic salt is further improved.

The invention relates to an agrobacterium tumefaciens-mediated cork tree genetic transformation method. The method comprises the following steps: preparing an infection solution from an agrobacterium solution; the method comprises the following steps: taking in-vitro cotyledons or stems as explants after cork tree seeds germinate into seedlings, infecting the cotyledons or stems of the cork tree by adopting an infection solution, and carrying out co-culture, recovery culture and screening culture after infection is completed, so as to obtain transgenic hairy roots or transgenic calluses. According to the scheme provided by the invention, stable transformation of balsa roots can be realized, verification of functional genes is realized, the functional genes exist in a living body form for a long time, and the genetic transformation efficiency of balsa is improved.

The invention discloses a multi-species mutualism-based mussel and fish polyculture ecological regulation and control method, and relates to the technical field of ecological culture, and the method comprises the following steps: S1, facility preparation; s2, putting species; s3, breeding regulation and control; s4, feeding control; and S5, performing final-period management. Through strict screening of the culture pond, thorough disinfection and scientific cultivation of water quality, an excellent starting end is created for culture, the disease risk is reduced, beneficial microbial communities enhance the self-cleaning capacity of a water body, the feeding quantity and proportion are accurately calculated, the ecological capacity is fully utilized, the fingerlings are reasonably matched, stable operation of an ecological system is guaranteed, and the ecological system is more intelligent and reliable through real-time monitoring and intelligent prediction. The feeding amount and the water changing frequency are adjusted in time, the water quality is effectively maintained, the breeding loss caused by water quality deterioration is reduced, grass carp feeding is adjusted according to the aquatic plant coverage rate, reasonable growth of aquatic plants is maintained, ecological balance is promoted, the win-win situation of ecological benefits and economic benefits is achieved, and an effective mode is provided for sustainable aquaculture.

A 16SrDNA sequence of the ruminant rumen-source bacillus ginseng geobacillus is shown as SEQ ID NO.1. A strain is collected from a ruminant rumen and inoculated to a culture medium with straw as a substrate for anaerobic culture and passage enrichment culture to obtain a dominant strain utilizing the straw, screening culture of cellulosic bacteria is performed, single colony selection and purification are performed, and the ruminant rumen-source bacillus ginseng geobacillus is obtained. The strain with the cellulose monodegradation capability is obtained. The bacillus panacis disclosed by the invention is high in adaptability, can effectively ferment feed in aerobic and anoxic environments, can quickly start fermentation, is high in stability in the fermentation process, improves the quality of the feed and reduces the feeding cost.

The invention relates to the technical field of transgenic breeding, in particular to an efficient genetic transformation method of Chinese orchid based on rhizomes. The method comprises the following steps: infecting a Chinese orchid rhizome by using agrobacterium carrying a target gene, and sequentially carrying out co-culture, recovery culture and screening culture to obtain a positive explant; and culturing the positive explant to obtain a positive seedling. The invention provides an efficient genetic transformation method based on rhizomes for the Chinese orchid for the first time, a Chinese orchid genetic transformation system is determined, and a basis is provided for transgenic breeding of the Chinese orchid.

The application discloses a method for improving genetic transformation rate of high-altitude meadow grass, comprising the following steps: (1) taking mature seeds of high-altitude meadow grass as explants to induce callus, and obtaining the callus; (2) adding a surfactant Silwet L-77 with a mass fraction of 3‰ of the bacterial liquid to the agrobacterium bacterial liquid carrying a hygromycin resistance gene, and obtaining a culture bacterial liquid; (3) placing the regenerated plant in a screening culture medium containing 40 mg / L of hygromycin resistance for culture, screening the survived plants, and obtaining the genetically transformed plants. The method can improve the transformation efficiency of the high-altitude meadow grass by about 4.54 times through adding the surfactant in the genetic transformation process and cooperating with the screening of the obtained differentiation culture medium.

The application provides a genetic transformation method of sugarcane variety Liucheng 1541 and belongs to the technical field of plant genetic engineering. The genetic transformation method is that leaf tissues of the sugarcane variety Liucheng 1541 are induced and cultured on a callus induction culture medium containing 2,4-dichlorophenoxyacetic acid and 6-benzylaminopurine; the obtained embryogenic callus is infected by agrobacterium; after co-cultivation and recovery culture, the obtained recovered callus is transferred to a screening culture medium containing glufosinate ammonium for screening culture; then the screened resistant embryogenic callus is inoculated on a differentiation culture medium containing glufosinate ammonium for differentiation culture; and after rooting culture, the transgenic plant of Liucheng 1541 is obtained. The method improves the embryogenic callus induction rate, solves the problem of callus browning in the tissue culture process of the variety Liucheng 1541, determines the glufosinate ammonium screening concentration of the embryogenic callus, and establishes the genetic transformation system of Liucheng 1541.

The invention belongs to the technical field of natural medicines, and particularly relates to a method for increasing the content of catharanthine in hairy roots of catharanthus roseus, which is characterized by comprising the following steps: extracting RNA (Ribonucleic Acid) of the catharanthus roseus, carrying out reverse transcription to obtain cDNA (Complementary Deoxyribonucleic Acid), carrying out PCR (Polymerase Chain Reaction) amplification by taking the cDNA as a template to respectively obtain GS (Glutathione S) and CS (Citrus S), with the nucleotide sequence of the GS as shown in SEQ ID NO.9 and the nucleotide sequence of the CS as shown in SEQ ID NO.10; gS and CS are jointly cloned into an overexpression vector, and a double-gene expression vector is obtained; infecting the catharanthus roseus by using the double-gene expression vector; the infected catharanthus roseus is sequentially subjected to screening culture and subculture, an inducer is added in the subculture process, and the inducer is dexamethasone. Through the method provided by the invention, the content of catharanthine in the catharanthus roseus can be increased by 67% or above. The method for increasing the content of the vinblastine in the hairy roots of the catharanthus roseus is established, and a foundation is laid for large-scale production of the direct precursor vinblastine of an anti-cancer drug through the hairy roots.

The invention discloses a construction method of an agrobacterium tumefaciens-mediated erigeron breviscapus genetic transformation system, which comprises the following steps: putting an erigeron breviscapus explant into a prepared transgenic infection solution containing recombinant plasmid 1300-EbGRF4-GFP agrobacterium tumefaciens, and sequentially carrying out vacuum infiltration transformation treatment, then respectively and sequentially placing in an erigeron breviscapus co-culture medium, a recovery culture medium and a screening culture medium for culturing to obtain new proliferated calluses; and transferring the new proliferated callus into a bud initial culture medium to induce budlets, culturing in a bud differentiation culture medium, differentiating into resistant seedlings, and transferring into a rooting culture medium to obtain a transgenic plant. The method disclosed by the invention has the advantages that the method is used for inducing calluses of the erigeron breviscapus and efficiently transforming agrobacterium tumefaciens mediation, so that the erigeron breviscapus can quickly obtain target characters, the self genetic barrier of the erigeron breviscapus can be broken through, and a new variety which is difficult to produce through a traditional breeding mode can be obtained.

The invention discloses a genetic transformation method suitable for a traditional Chinese medicine divaricate saposhnikovia root, and belongs to the technical field of biology. According to the genetic transformation method, the leaf is taken as an infected object, the infected leaf is differentiated to obtain the callus, and then the regenerated seedling is differentiated, so that the test period of genetic transformation is shortened, and the differentiation rate of the regenerated seedling is improved. According to the method, the bacteriostasis rate, the differentiation rate and the positive conversion rate are improved through callus induction, bacteriostasis and resistance screening culture media of specific mass concentration components and limitation of the infection time of agrobacterium tumefaciens, an efficient and stable genetic transformation system of the radix saposhnikoviae is established, and the method has a good application prospect in precise improvement of variety characters of the radix saposhnikoviae. And a support is provided for cultivating a high-quality, high-yield and multi-resistance new divaricate saposhnikovia root variety.

The invention belongs to the technical field of disease control in aquaculture, and particularly relates to a hippocampus enteritis pathogen separation and identification and medication screening method based on a high-throughput sequencing technology, which comprises the following steps: collecting intestinal tissues of a diseased hippocampus and a healthy hippocampus, respectively preparing tissue homogenate, and freezing for preservation; the method comprises the following steps: extracting DNA (Deoxyribose Nucleic Acid) of microorganisms of an intestinal tissue sample, performing 16SrDNA high-throughput sequencing, analyzing intestinal flora composition, and determining dominant bacteria; selecting and screening a culture medium according to dominant bacteria, and performing pathogenic bacterium separation and purification on intestinal tissues of the diseased hippocampus; dNA of the purified strain is extracted, 16S rDNA amplification and sequencing are carried out, and the species of pathogenic bacteria are identified through sequence comparison; according to the method, the diversity and abundance differences of microorganisms in the sick hippocampus and the healthy hippocampus are compared through a high-throughput sequencing technology, and targeted separation, identification and drug sensitivity screening are further carried out by taking statistically significant difference bacteria as the guidance of potential pathogenic bacteria.

The invention discloses a neutralizing antibody with an AQP3 channel blocking function as well as a preparation method and application thereof, and belongs to the technical field of biological medicines. The neutralizing antibody can be specifically combined with an extracellular domain of AQP3, after combination, a water channel and a small molecule transport function of the AQP3 are blocked through steric hindrance and conformation change, and meanwhile, antigen combination specificity is reserved. The preparation method is realized through'recombinant antigen-immunization-B cell screening culture-recombinant antibody expression and affinity purification-ELISA detection ', and the functions of the antibody are ensured. The antibody can be efficiently combined with AQP3 positive cells, is strong in targeting property and high in specificity (the serum titer reaches 1: 1024 * 10, and the purified antibody titer is greater than or equal to 1: 128 * 10), and can be independently used or combined with a nano-carrier for treating related diseases, so that the technical bottleneck that a common AQP3 antibody can only be combined and cannot efficiently play a blocking role is solved.

The invention discloses a method for constructing a genetic transformation system of cymbidium kanran, which belongs to the technical field of plant genetic engineering, and comprises the following steps of: carrying out monoclonal propagation on rhizomes after germination of cymbidium kanran seeds to obtain vegetative propagules with stable characters, taking the vegetative propagules as transformation receptors, taking GFP (Green Fluorescent Protein) as marker genes, and constructing the genetic transformation system of cymbidium kanran. The method comprises the following steps: adding agrobacterium EHA105 into a YEB liquid culture medium containing 50 mg / L of kanamycin and 25 mg / L of rifampicin as a dip dyeing solution; placing the injured transformation receptor in dip dyeing for transformation, and then carrying out regeneration culture. The invention relates to a method for carrying out genetic transformation on cymbidium kanran by utilizing the innovative receptor and the screening culture medium, and the method can realize efficient and stable genetic transformation without genotype dependence and can solve the problem that the genetic transformation of cymbidium kanran varieties is difficult to carry out. According to the invention, an efficient genetic transformation and regeneration system of cymbidium kanran is established for the first time, and a foundation is laid for verification of related functional genes of cymbidium kanran and further development of biological research of cymbidium transgenic breeding and the like.

This invention belongs to the field of microbial technology, and particularly relates to a screening culture medium for precise intestinal flora transplantation, its preparation method, and a method for enriching Enterobacteriaceae bacteria. The application of the composition provided by this invention in the preparation of intestinal flora screening culture media, through the synergistic effect of the basic nutritional conditions provided by the oligofructose, chlorogenic acid, and CDC anaerobic agar basal medium, enables effective screening and enrichment of Enterobacteriaceae bacteria in fecal samples under in vitro culture conditions, showing excellent application prospects in the isolation, purification, and functional studies of Enterobacteriaceae bacteria.

The invention discloses a stable genetic transformation method of floating moss. The genetic transformation method comprises the following steps: (1) crushing thallus tissues of the floating moss, (2) pre-culturing explants, (3) infecting and co-culturing, (4) screening and culturing for the first time, (5) screening and culturing for the second time, (6) enlarging and culturing, and (7) verifying transformation. According to the genetic transformation method, a thallus of floating moss is taken as a starting explant, an agrobacterium-mediated hygromycin and kanamycin screening transformation system of the floating moss is established for the first time, the blank of a floating moss genetic transformation system is filled, and experimental results show that after multi-generation culture, a transformed plant can still stably express a target gene. The method has the advantages of high transformation efficiency and simplicity in operation, and can be used for subsequent genetic transformation research of unknown floating moss genes. The method has a wide application prospect.

The invention provides a genetic transformation method of an excellent sugarcane variety Liucheng 1541, and belongs to the technical field of plant genetic engineering.The genetic transformation method comprises the steps that leaf tissues of the sugarcane variety Liucheng 1541 are taken and subjected to induction culture on a callus induction culture medium containing 2, 4-dichlorphenoxyacetic acid and 6-benzylaminopurine; the method comprises the following steps: infecting embryogenic calluses with agrobacterium, co-culturing, carrying out recovery culture, transferring the recovered calluses to a screening culture medium containing glufosinate-ammonium, carrying out screening culture, inoculating the screened resistant embryogenic calluses to a differential culture medium containing glufosinate-ammonium, carrying out differentiation culture, and carrying out rooting culture to obtain the glufosinate-ammonium resistant embryogenic callus. The transgenic plant of the Liucheng 1541 is obtained. According to the method, the embryogenic callus induction rate is increased, the problem of callus browning in the variety Liucheng 1541 tissue culture process is solved, the glufosinate-ammonium screening concentration of the embryogenic callus is determined, and a Liucheng 1541 genetic transformation system is determined.

The invention discloses an agrobacterium tumefaciens-mediated soybean sprout tip transformation method. The method comprises the following steps of explant infection and co-culture, bud induction and screening culture, elongation recovery and rooting culture and transgenic plant domestication culture. According to the method, the soybean sprout tips are selected as the explants, the soybean genetic transformation process is simplified, direct budding of apical meristem and synchronous rooting of underground parts can be achieved, only one month is needed from explant infection to obtaining of a complete regenerated transgenic plant with a developed root system, the transformation efficiency is high, the soybean transformation period is greatly shortened, and the method is suitable for large-scale popularization and application. The cost is effectively reduced. Meanwhile, the problem of soybean genotype limitation and the problem that northeast varieties bloom in advance in the tissue culture stage are solved, the method is applied to more than twenty main cultivated varieties difficult to transform, the transformation efficiency is high and stable, the highest efficiency can reach 81%, and reliable technical support is provided for soybean basic research and gene editing breeding work.

The invention discloses a screening and domesticating method of an L-alanine fermentation strain, which comprises the following steps: (1) preparing a screening culture medium; (2) screening: carrying out primary culture on the L-alanine fermentation strain by adopting the screening culture medium to obtain a primary culture L-alanine fermentation strain liquid; inoculating the primarily-cultured L-alanine zymophyte liquid into a newly-screened culture medium according to the inoculum size of 4-5%, and carrying out secondary culture to obtain a secondarily-cultured L-alanine zymophyte liquid; repeating the steps for 5-7 times to obtain final fifth-seventh generations of culture L-alanine zymophyte liquid; (3) preparing a domestication culture medium; (4) domestication; the screened and domesticated zymophyte has certain acid resistance and alkali resistance, can keep activity and stably grow and ferment in a certain range of acid environment, and can be helpful for ensuring the yield of L-alanine in the fermentation process of the zymophyte through relatively strong pH adaptability.

The invention discloses a pseudo-sorghum genetic transformation method and application. The pseudo-sorghum genetic transformation method comprises the steps of pseudo-sorghum mature seed explant pretreatment, induction culture, gene gun transformation, screening culture, differentiation culture, budding culture, rooting culture and the like. According to the method disclosed by the invention, the callus is successfully induced from the pseudo-sorghum seed immature embryo, the inductivity is high, a simple and efficient genetic transformation method is established, target editing of a pseudo-sorghum gene editing plant can be realized, and the transformation efficiency and the regeneration capacity are high.

The invention discloses a screening culture medium and a preparation method and application thereof, the screening culture medium comprises a basic culture medium and additives, and the additives comprise amphotericin B, vancomycin, ox bile salt, sodium deoxycholate and No.3 bile salt. According to the screening culture medium provided by the invention, a special infectious microbe inhibitor is added on the basis of a trisaccharide iron culture medium, so that most gram-negative enterobacter, staphylococcus aureus and part of fungi except salmonella enteritis subspecies and shigella cannot grow; the interference of the infectious microbes on the detection of the salmonella enteritis subspecies and the shigella is avoided, so that the fluorescent probe can be applied to the detection of the salmonella enteritis subspecies and the shigella in a clinical anus swab, and the sensitivity of color development is improved.

A yeast dual-expression vector and a construction method therefor, a protein-protein interaction identification method and the use. The yeast dual-expression vector allows two exogenous genes to be cloned into the same vector and expressed simultaneously in yeast, thereby enabling interaction identification. The present invention greatly reduces the procedures in original yeast two-hybrid systems, such as plasmid construction and extraction, library preparation, yeast mating and screening culture, and significantly improves the identification efficiency of protein-protein interaction.

The invention provides a construction method and application of a schizophrenia phenotype zebrafish model, and relates to the technical field of genetic engineering.According to the construction method, RNP mixed liquid containing sgRNA and Cas9 protein is introduced into zebrafish fertilized eggs, screening culture is conducted, knockout of a zebrafish pccb gene is creatively achieved, and the schizophrenia phenotype zebrafish model is obtained. The zebrafish model constructed by the invention has the advantages of high detection flux, short detection period, high detection sensitivity and few ethical limitations on detection carriers when being used for researching medicines related to mental diseases, and has wide application value for rapidly screening and evaluating schizophrenia treatment medicines and natural efficacy factors.

The application discloses a jujube genetic transformation method using anther-induced callus as a receptor, comprising the following steps: culturing sterile anthers to obtain callus; constructing a plant recombinant overexpression vector containing a target gene and transferring the plant recombinant overexpression vector into agrobacterium to obtain an agrobacterium immersion liquid; immersing the callus in the agrobacterium immersion liquid, and then co-culturing the immersed callus under dark conditions; and then placing the callus on a culture medium containing hygromycin for screening, so that a homozygous resistant callus can be obtained after 20-30 days of screening culture. The application firstly uses the anther-induced callus of Jing 39 jujube as a receptor to establish a method of the jujube genetic transformation system, the method has a high transformation frequency, and meanwhile, the method can be assisted by 35S::EGFP fluorescent reporter gene marking for screening, and is convenient and fast. The method provides convenience for jujube gene function analysis and jujube genetic improvement.

The invention belongs to the technical field of wine brewing microorganisms, and particularly relates to screening culture and application of bacillus hexanoate producing bacillus. According to the present invention, the caproic acid producing bacillus strain LLMJ-JS-1 is derived from the strong aromatic white wine cellar bottom mud, belongs to the wild type strain, and has characteristics of stable genome and stable phenotype, and the caproic acid producing bacillus strain LLMJ-JS-1 can be used to produce the caproic acid producing bacillus strain LLMJ-JS-1, such that the caproic acid producing bacillus strain LLMJ-JS-1, the acid production level in a Pasteur synthesis liquid culture solution reaches up to 10.9 g / L, the caproic acid production capacity is remarkably superior to that of a traditional strain, and the problems that an existing strain is low in acid production amount and insufficient in acid production stability are effectively solved. The method has good adaptive capacity in the pit environment, provides a solid scientific foundation for production and application of the method in pit mud production and pit maintenance liquid preparation, and has remarkable potential and wide application prospects in the aspect of improving the quality of base liquor.

The invention relates to the technical field of biology, and provides a novel technology for rapidly screening and identifying high-yield poly-gamma-glutamic acid bacillus. The method comprises the following steps: preparing a solid screening culture medium, sterilizing, subpackaging into a square sterile culture dish with scales, diluting a to-be-separated substance, coating the solidified culture medium with the diluted to-be-separated substance, culturing for 24-96 hours, and observing whether a liquid product exists on a cover plate or not; if so, determining that the solid screening culture medium region corresponding to the region contains the high-yield poly-gamma-glutamic acid strain, and determining the single colony of the high-yield poly-gamma-glutamic acid through a sterilization gun head wire drawing experiment. According to the method, the traditional process of screening the high-yield gamma-polyglutamic acid bacillus is simplified, and the workload in the screening process is greatly reduced.

The invention discloses a suspension culture cell monoclonal screening culture method. The method comprises the following steps: selecting cells under suspension culture conditions and inducing the cells to recover adherence; separating the single cells developed into obvious clones, and carrying out radioactive ray irradiation on the remaining cells, so that the remaining cells lose the multiplication capacity and become feeder layer cells; culturing the separated monoclonal cells, recording the culture time and culture environment, establishing a high-density cell bank, and establishing a growth time prediction formula for the multiple monoclonal cells according to the culture time; and calculating the specific time for culturing the required number of cells according to the growth time prediction formula established in the step S3. According to the method, the remaining cells are subjected to radioactive ray irradiation and are converted into feeder layer cells with important effects, so that the waste of cell resources is avoided, the flexible utilization of the cell resources is realized, and the treatment cost of the remaining cells is also remarkably reduced.

The utility model provides a high-yield ergothioneine strain screening culture bin relates to strain screening technical field, including base, culture bin and cover plate, the upper end of base is provided with the jacket seat, in the jacket seat is provided with the thermal insulation layer, the upper part of the thermal insulation layer is provided with the neck, and the culture bin is embedded in the neck, the cover plate is provided with the cover plate. The upper end of the cultivation bin is covered with a cover plate, the cover plate is detachably connected with the cultivation bin, the cultivation bin is detachably connected with the base, multiple sets of anti-slip strips are arranged on the outer ring face of the base, the anti-slip strips are of a semi-cylindrical anti-slip strip structure, and an anti-slip pad is arranged on the bottom face of the base and made of silica gel. The heat preservation layer is made of heat preservation cotton materials, and the outer sleeve base is made of explosion-proof glass materials. The strain screening device is safe and stable in hand taking and placing, tight isolation is achieved, heat preservation and heat insulation are better kept in the screening process, the influence of external environment temperature factors is reduced, therefore, the strain screening quality is guaranteed, and the strain screening device is more efficient and practical.

The present invention discloses a method for Agrobacterium-mediated genetic transformation of Erianthus fulvus in sugarcane complex, belonging to the technical field of plant tissue culture and genetic transformation. Through Agrobacterium-mediated transformation, the present invention develops a genetic transformation system for diploid Erianthus fulvus in sugarcane complex. By optimizing the callus induction medium of Erianthus fulvus, the embryogenicity of Erianthus fulvus callus is improved; through vacuum infiltration, the infection intensity of Erianthus fulvus callus is strengthened; through the recovery culture and subculture screening culture of callus, the transformation efficiency is increased. Using the genetic transformation method of the present invention, when transforming 70-90 pieces of embryogenic callus of Erianthus fulvus, 10-14 transgenic lines can be obtained, and the transformation efficiency is as high as 14.21%. The present invention constructs a genetic transformation system for Erianthus fulvus for the first time, laying a foundation for further developing Erianthus fulvus into a model plant of sugarcane complex and providing technical support for carrying out research on functional genes of sugarcane complex.

The utility model discloses a melon planting resource salt tolerance gradient screening culture device, which belongs to the technical field of plant culture screening equipment, and comprises a culture box, a culture drawer arranged in the culture box, a culture basin arranged on the culture drawer, a fixing mechanism and an adjusting mechanism, the fixing mechanism comprises a sliding groove block fixed to the inner wall of the incubator and a sliding block fixed to the outer wall of the culture drawer, the sliding block is in sliding fit with the sliding groove block, and the fixing mechanism further comprises a buckle installed in the sliding groove block in an up-down sliding mode and a limiting ring used for preventing the buckle from being disengaged. The problems that the culture drawer is not firmly fixed and inconvenient to store and take, and the humidity in the box is difficult to control are solved, fixation is stable, operation is convenient and fast, and the disease risk can be reduced.

The invention discloses a method for detecting the editing efficiency of a plant polygene editing vector. Comprising the following steps: (1) designing sgRNA according to a plurality of target genes of a plant to be edited and constructing a plant multi-gene editing vector; (2) transforming the constructed plant polygene editing vector into a hairy root of a plant; (3) identifying hairy roots of positive transgenic plants; (4) detecting the editing efficiency of the plant polygene editing vector; wherein the transformation comprises the following steps: (a) preparing an agrobacterium mycoderm; (b) scratching the root tips of the germinated plant seeds in a 1 / 2CO liquid culture medium to generate wounds; (c) scratching the wound of the seed on an agrobacterium pellicle, and culturing on a 1 / 2CO solid culture medium; and (d) after the wound of the root tip expands, transferring into a 1 / 2 screening culture medium, and continuously culturing to obtain the hairy root of the transgenic plant. Through the detection method, the gRNA can be more effectively screened, so that efficient gene editing is realized, and the experimental period is greatly shortened.