76 results about "Screening cultures" patented technology

The invention discloses a construction method of an agrobacterium tumefaciens mediated masson pine genetic transformation system, and belongs to the technical field of genetic engineering. According to the construction method of the agrobacterium tumefaciens-mediated masson pine genetic transformation system, immature masson pine cones are collected, embryogenic calluses are induced and cultured, the embryogenic calluses of the masson pine in a PEM III stage are used as a material, suspension pre-culture is performed to obtain an acceptor material, and the acceptor material is subjected to genetic transformation to obtain the agrobacterium tumefaciens-mediated masson pine genetic transformation system. The method comprises the following steps: firstly, carrying out tissue culture on a transgenic somatic embryo, infecting by adopting an agrobacterium infection solution with an OD600 value of 0.2-0.6, carrying out co-culture, recovery culture and screening culture, carrying out histochemical staining by adopting GUS, carrying out molecular identification to obtain a transgenic embryogenic callus, and carrying out maturation treatment to finally obtain the transgenic somatic embryo. The masson pine genetic transformation system established by the invention is stable in transformation performance and high in transformation positive rate, and when an acceptor material is masson pine embryonic callus with the genotype of 1-3-5, the transformation positive rate is as high as 100%.

The invention discloses a method for screening and culturing active plastic degrading bacteria based on combination of Raman spectrum and heavy water labeling, which realizes rapid screening of high-activity plastic degrading bacteria within 7 days by utilizing intestinal microorganisms under the condition of inorganic salt culture incubation with plastic as a unique carbon source. The method comprises the following steps: dissecting and homogenizing intestinal tracts of tenebrio molitor fed with plastics under a sterile condition, harvesting enteric microorganisms through iohexol density gradient centrifugation, incubating the enteric microorganisms in an inorganic salt culture solution containing heavy water and plastics to serve as a treatment group, centrifugally cleaning an incubated to-be-detected substance, and performing Raman detection, the high-activity plastic degrading bacteria can be sorted for further downstream sequencing and culture. The method is rapid, efficient, easy and convenient to operate and suitable for rapidly screening, identifying and culturing the high-activity plastic degrading bacteria from a complex natural environment, and development of plastic biodegradation and harmless treatment processes is facilitated.

The invention relates to the technical field of protein interaction in yeast, in particular to a yeast double-expression vector, a construction method thereof, a protein interaction identification method and application. According to the vector, two exogenous genes can be cloned in the same vector, and the two exogenous genes can be expressed in yeast at the same time, so that interaction identification can be carried out. The work of plasmid construction and extraction, library preparation, yeast hybridization, screening culture and the like of an original yeast two-hybrid system is greatly reduced, and the identification efficiency of protein interaction is remarkably improved.

The invention relates to the technical field of plant tissue culture and plant transgenosis, in particular to a method for co-transformation of an LcSUCLA alpha-LcSUCLA beta-pEGFC binary expression vector and Leymus chinensis double genes. The method comprises the following steps: obtaining leymus chinensis tissue culture sterile calluses, transforming agrobacterium competent by a freeze thawing method, activating agrobacterium tumefaciens, infecting the leymus chinensis calluses and co-culturing, screening and culturing plant transformation materials, differentiating and screening a culture medium and culture conditions, and differentiating and screening a culture medium and culture conditions as well as a rooting culture medium and culture conditions. According to the invention, double-gene co-transformation of leymus chinensis is realized, and a leymus chinensis double-transgenic plant is obtained. The cotransformation of the LcSUCLA alpha and the LcSUCLA beta in the leymus chinensis can synthesize more succinic acid than the single transformation, so that the tolerance of the leymus chinensis under the stress of basic salt is further improved.

The invention relates to an agrobacterium tumefaciens-mediated cork tree genetic transformation method. The method comprises the following steps: preparing an infection solution from an agrobacterium solution; the method comprises the following steps: taking in-vitro cotyledons or stems as explants after cork tree seeds germinate into seedlings, infecting the cotyledons or stems of the cork tree by adopting an infection solution, and carrying out co-culture, recovery culture and screening culture after infection is completed, so as to obtain transgenic hairy roots or transgenic calluses. According to the scheme provided by the invention, stable transformation of balsa roots can be realized, verification of functional genes is realized, the functional genes exist in a living body form for a long time, and the genetic transformation efficiency of balsa is improved.

The invention discloses a multi-species mutualism-based mussel and fish polyculture ecological regulation and control method, and relates to the technical field of ecological culture, and the method comprises the following steps: S1, facility preparation; s2, putting species; s3, breeding regulation and control; s4, feeding control; and S5, performing final-period management. Through strict screening of the culture pond, thorough disinfection and scientific cultivation of water quality, an excellent starting end is created for culture, the disease risk is reduced, beneficial microbial communities enhance the self-cleaning capacity of a water body, the feeding quantity and proportion are accurately calculated, the ecological capacity is fully utilized, the fingerlings are reasonably matched, stable operation of an ecological system is guaranteed, and the ecological system is more intelligent and reliable through real-time monitoring and intelligent prediction. The feeding amount and the water changing frequency are adjusted in time, the water quality is effectively maintained, the breeding loss caused by water quality deterioration is reduced, grass carp feeding is adjusted according to the aquatic plant coverage rate, reasonable growth of aquatic plants is maintained, ecological balance is promoted, the win-win situation of ecological benefits and economic benefits is achieved, and an effective mode is provided for sustainable aquaculture.

A 16SrDNA sequence of the ruminant rumen-source bacillus ginseng geobacillus is shown as SEQ ID NO.1. A strain is collected from a ruminant rumen and inoculated to a culture medium with straw as a substrate for anaerobic culture and passage enrichment culture to obtain a dominant strain utilizing the straw, screening culture of cellulosic bacteria is performed, single colony selection and purification are performed, and the ruminant rumen-source bacillus ginseng geobacillus is obtained. The strain with the cellulose monodegradation capability is obtained. The bacillus panacis disclosed by the invention is high in adaptability, can effectively ferment feed in aerobic and anoxic environments, can quickly start fermentation, is high in stability in the fermentation process, improves the quality of the feed and reduces the feeding cost.

The invention discloses a soybean genetic transformation method for improving positive transformation rate, which comprises the following steps of: infecting a soybean explant with a recombinant expression vector containing a target gene and a marker gene through agrobacterium liquid; an ALS inhibitor is added into a bud induction culture medium and a bud elongation culture medium to improve screening culture of the positive conversion rate of the explants, and a transformant is obtained. According to the soybean genetic transformation method which takes the gene cloned from the cynodon dactylon as the marker gene and the ALS inhibitor as the screening agent, the positive transformation rate is increased to 6% or above, the maximum is 12.05%, and the soybean transformation efficiency is remarkably improved.

The invention relates to the technical field of transgenic breeding, in particular to an efficient genetic transformation method of Chinese orchid based on rhizomes. The method comprises the following steps: infecting a Chinese orchid rhizome by using agrobacterium carrying a target gene, and sequentially carrying out co-culture, recovery culture and screening culture to obtain a positive explant; and culturing the positive explant to obtain a positive seedling. The invention provides an efficient genetic transformation method based on rhizomes for the Chinese orchid for the first time, a Chinese orchid genetic transformation system is determined, and a basis is provided for transgenic breeding of the Chinese orchid.

The application discloses a method for improving genetic transformation rate of high-altitude meadow grass, comprising the following steps: (1) taking mature seeds of high-altitude meadow grass as explants to induce callus, and obtaining the callus; (2) adding a surfactant Silwet L-77 with a mass fraction of 3‰ of the bacterial liquid to the agrobacterium bacterial liquid carrying a hygromycin resistance gene, and obtaining a culture bacterial liquid; (3) placing the regenerated plant in a screening culture medium containing 40 mg / L of hygromycin resistance for culture, screening the survived plants, and obtaining the genetically transformed plants. The method can improve the transformation efficiency of the high-altitude meadow grass by about 4.54 times through adding the surfactant in the genetic transformation process and cooperating with the screening of the obtained differentiation culture medium.

The invention relates to the technical field of microbial resource utilization of Maotai-flavor yeast for making hard liquor, in particular to a screening device for ester-producing yeast in Maotai-flavor yeast for making hard liquor, which comprises a culture device, the culture device is internally provided with a stirring device and a temperature control device, and the top of the culture device is provided with a bacterial powder adding device; the input end of the single-bacterium primary screening culture unit is communicated with the output end of the culture device through a first plate culture unit, and the single-bacterium primary screening culture unit is respectively communicated with a second plate culture unit, an esterifying power detection device and a single-bacterium secondary screening culture unit; the single-bacterium re-screening culture unit is respectively communicated with a flavor detection device, a total ester detection device and a single-bacterium re-screening culture unit. According to the method, 10-12-degree Bx corn saccharified mash and saccharified liquid taking wheat as a raw material are introduced as culture media, so that the fermentation environment of Maotai-flavor yeast for making hard liquor can be better simulated, richer nutrient substances can be provided for the ester-producing yeast, and the growth and esterification capability of the ester-producing yeast are promoted.

The application provides a genetic transformation method of sugarcane variety Liucheng 1541 and belongs to the technical field of plant genetic engineering. The genetic transformation method is that leaf tissues of the sugarcane variety Liucheng 1541 are induced and cultured on a callus induction culture medium containing 2,4-dichlorophenoxyacetic acid and 6-benzylaminopurine; the obtained embryogenic callus is infected by agrobacterium; after co-cultivation and recovery culture, the obtained recovered callus is transferred to a screening culture medium containing glufosinate ammonium for screening culture; then the screened resistant embryogenic callus is inoculated on a differentiation culture medium containing glufosinate ammonium for differentiation culture; and after rooting culture, the transgenic plant of Liucheng 1541 is obtained. The method improves the embryogenic callus induction rate, solves the problem of callus browning in the tissue culture process of the variety Liucheng 1541, determines the glufosinate ammonium screening concentration of the embryogenic callus, and establishes the genetic transformation system of Liucheng 1541.

The invention belongs to the technical field of natural medicines, and particularly relates to a method for increasing the content of catharanthine in hairy roots of catharanthus roseus, which is characterized by comprising the following steps: extracting RNA (Ribonucleic Acid) of the catharanthus roseus, carrying out reverse transcription to obtain cDNA (Complementary Deoxyribonucleic Acid), carrying out PCR (Polymerase Chain Reaction) amplification by taking the cDNA as a template to respectively obtain GS (Glutathione S) and CS (Citrus S), with the nucleotide sequence of the GS as shown in SEQ ID NO.9 and the nucleotide sequence of the CS as shown in SEQ ID NO.10; gS and CS are jointly cloned into an overexpression vector, and a double-gene expression vector is obtained; infecting the catharanthus roseus by using the double-gene expression vector; the infected catharanthus roseus is sequentially subjected to screening culture and subculture, an inducer is added in the subculture process, and the inducer is dexamethasone. Through the method provided by the invention, the content of catharanthine in the catharanthus roseus can be increased by 67% or above. The method for increasing the content of the vinblastine in the hairy roots of the catharanthus roseus is established, and a foundation is laid for large-scale production of the direct precursor vinblastine of an anti-cancer drug through the hairy roots.

The invention discloses a construction method of an agrobacterium tumefaciens-mediated erigeron breviscapus genetic transformation system, which comprises the following steps: putting an erigeron breviscapus explant into a prepared transgenic infection solution containing recombinant plasmid 1300-EbGRF4-GFP agrobacterium tumefaciens, and sequentially carrying out vacuum infiltration transformation treatment, then respectively and sequentially placing in an erigeron breviscapus co-culture medium, a recovery culture medium and a screening culture medium for culturing to obtain new proliferated calluses; and transferring the new proliferated callus into a bud initial culture medium to induce budlets, culturing in a bud differentiation culture medium, differentiating into resistant seedlings, and transferring into a rooting culture medium to obtain a transgenic plant. The method disclosed by the invention has the advantages that the method is used for inducing calluses of the erigeron breviscapus and efficiently transforming agrobacterium tumefaciens mediation, so that the erigeron breviscapus can quickly obtain target characters, the self genetic barrier of the erigeron breviscapus can be broken through, and a new variety which is difficult to produce through a traditional breeding mode can be obtained.

The invention discloses a genetic transformation method suitable for a traditional Chinese medicine divaricate saposhnikovia root, and belongs to the technical field of biology. According to the genetic transformation method, the leaf is taken as an infected object, the infected leaf is differentiated to obtain the callus, and then the regenerated seedling is differentiated, so that the test period of genetic transformation is shortened, and the differentiation rate of the regenerated seedling is improved. According to the method, the bacteriostasis rate, the differentiation rate and the positive conversion rate are improved through callus induction, bacteriostasis and resistance screening culture media of specific mass concentration components and limitation of the infection time of agrobacterium tumefaciens, an efficient and stable genetic transformation system of the radix saposhnikoviae is established, and the method has a good application prospect in precise improvement of variety characters of the radix saposhnikoviae. And a support is provided for cultivating a high-quality, high-yield and multi-resistance new divaricate saposhnikovia root variety.

The invention belongs to the technical field of disease control in aquaculture, and particularly relates to a hippocampus enteritis pathogen separation and identification and medication screening method based on a high-throughput sequencing technology, which comprises the following steps: collecting intestinal tissues of a diseased hippocampus and a healthy hippocampus, respectively preparing tissue homogenate, and freezing for preservation; the method comprises the following steps: extracting DNA (Deoxyribose Nucleic Acid) of microorganisms of an intestinal tissue sample, performing 16SrDNA high-throughput sequencing, analyzing intestinal flora composition, and determining dominant bacteria; selecting and screening a culture medium according to dominant bacteria, and performing pathogenic bacterium separation and purification on intestinal tissues of the diseased hippocampus; dNA of the purified strain is extracted, 16S rDNA amplification and sequencing are carried out, and the species of pathogenic bacteria are identified through sequence comparison; according to the method, the diversity and abundance differences of microorganisms in the sick hippocampus and the healthy hippocampus are compared through a high-throughput sequencing technology, and targeted separation, identification and drug sensitivity screening are further carried out by taking statistically significant difference bacteria as the guidance of potential pathogenic bacteria.

The invention discloses a neutralizing antibody with an AQP3 channel blocking function as well as a preparation method and application thereof, and belongs to the technical field of biological medicines. The neutralizing antibody can be specifically combined with an extracellular domain of AQP3, after combination, a water channel and a small molecule transport function of the AQP3 are blocked through steric hindrance and conformation change, and meanwhile, antigen combination specificity is reserved. The preparation method is realized through'recombinant antigen-immunization-B cell screening culture-recombinant antibody expression and affinity purification-ELISA detection ', and the functions of the antibody are ensured. The antibody can be efficiently combined with AQP3 positive cells, is strong in targeting property and high in specificity (the serum titer reaches 1: 1024 * 10, and the purified antibody titer is greater than or equal to 1: 128 * 10), and can be independently used or combined with a nano-carrier for treating related diseases, so that the technical bottleneck that a common AQP3 antibody can only be combined and cannot efficiently play a blocking role is solved.

The invention discloses a method for constructing a genetic transformation system of cymbidium kanran, which belongs to the technical field of plant genetic engineering, and comprises the following steps of: carrying out monoclonal propagation on rhizomes after germination of cymbidium kanran seeds to obtain vegetative propagules with stable characters, taking the vegetative propagules as transformation receptors, taking GFP (Green Fluorescent Protein) as marker genes, and constructing the genetic transformation system of cymbidium kanran. The method comprises the following steps: adding agrobacterium EHA105 into a YEB liquid culture medium containing 50 mg / L of kanamycin and 25 mg / L of rifampicin as a dip dyeing solution; placing the injured transformation receptor in dip dyeing for transformation, and then carrying out regeneration culture. The invention relates to a method for carrying out genetic transformation on cymbidium kanran by utilizing the innovative receptor and the screening culture medium, and the method can realize efficient and stable genetic transformation without genotype dependence and can solve the problem that the genetic transformation of cymbidium kanran varieties is difficult to carry out. According to the invention, an efficient genetic transformation and regeneration system of cymbidium kanran is established for the first time, and a foundation is laid for verification of related functional genes of cymbidium kanran and further development of biological research of cymbidium transgenic breeding and the like.

This invention belongs to the field of microbial technology, and particularly relates to a screening culture medium for precise intestinal flora transplantation, its preparation method, and a method for enriching Enterobacteriaceae bacteria. The application of the composition provided by this invention in the preparation of intestinal flora screening culture media, through the synergistic effect of the basic nutritional conditions provided by the oligofructose, chlorogenic acid, and CDC anaerobic agar basal medium, enables effective screening and enrichment of Enterobacteriaceae bacteria in fecal samples under in vitro culture conditions, showing excellent application prospects in the isolation, purification, and functional studies of Enterobacteriaceae bacteria.

The invention discloses a stable genetic transformation method of floating moss. The genetic transformation method comprises the following steps: (1) crushing thallus tissues of the floating moss, (2) pre-culturing explants, (3) infecting and co-culturing, (4) screening and culturing for the first time, (5) screening and culturing for the second time, (6) enlarging and culturing, and (7) verifying transformation. According to the genetic transformation method, a thallus of floating moss is taken as a starting explant, an agrobacterium-mediated hygromycin and kanamycin screening transformation system of the floating moss is established for the first time, the blank of a floating moss genetic transformation system is filled, and experimental results show that after multi-generation culture, a transformed plant can still stably express a target gene. The method has the advantages of high transformation efficiency and simplicity in operation, and can be used for subsequent genetic transformation research of unknown floating moss genes. The method has a wide application prospect.

The invention provides a genetic transformation method of an excellent sugarcane variety Liucheng 1541, and belongs to the technical field of plant genetic engineering.The genetic transformation method comprises the steps that leaf tissues of the sugarcane variety Liucheng 1541 are taken and subjected to induction culture on a callus induction culture medium containing 2, 4-dichlorphenoxyacetic acid and 6-benzylaminopurine; the method comprises the following steps: infecting embryogenic calluses with agrobacterium, co-culturing, carrying out recovery culture, transferring the recovered calluses to a screening culture medium containing glufosinate-ammonium, carrying out screening culture, inoculating the screened resistant embryogenic calluses to a differential culture medium containing glufosinate-ammonium, carrying out differentiation culture, and carrying out rooting culture to obtain the glufosinate-ammonium resistant embryogenic callus. The transgenic plant of the Liucheng 1541 is obtained. According to the method, the embryogenic callus induction rate is increased, the problem of callus browning in the variety Liucheng 1541 tissue culture process is solved, the glufosinate-ammonium screening concentration of the embryogenic callus is determined, and a Liucheng 1541 genetic transformation system is determined.

The invention discloses an agrobacterium tumefaciens-mediated soybean sprout tip transformation method. The method comprises the following steps of explant infection and co-culture, bud induction and screening culture, elongation recovery and rooting culture and transgenic plant domestication culture. According to the method, the soybean sprout tips are selected as the explants, the soybean genetic transformation process is simplified, direct budding of apical meristem and synchronous rooting of underground parts can be achieved, only one month is needed from explant infection to obtaining of a complete regenerated transgenic plant with a developed root system, the transformation efficiency is high, the soybean transformation period is greatly shortened, and the method is suitable for large-scale popularization and application. The cost is effectively reduced. Meanwhile, the problem of soybean genotype limitation and the problem that northeast varieties bloom in advance in the tissue culture stage are solved, the method is applied to more than twenty main cultivated varieties difficult to transform, the transformation efficiency is high and stable, the highest efficiency can reach 81%, and reliable technical support is provided for soybean basic research and gene editing breeding work.

The invention discloses a screening and domesticating method of an L-alanine fermentation strain, which comprises the following steps: (1) preparing a screening culture medium; (2) screening: carrying out primary culture on the L-alanine fermentation strain by adopting the screening culture medium to obtain a primary culture L-alanine fermentation strain liquid; inoculating the primarily-cultured L-alanine zymophyte liquid into a newly-screened culture medium according to the inoculum size of 4-5%, and carrying out secondary culture to obtain a secondarily-cultured L-alanine zymophyte liquid; repeating the steps for 5-7 times to obtain final fifth-seventh generations of culture L-alanine zymophyte liquid; (3) preparing a domestication culture medium; (4) domestication; the screened and domesticated zymophyte has certain acid resistance and alkali resistance, can keep activity and stably grow and ferment in a certain range of acid environment, and can be helpful for ensuring the yield of L-alanine in the fermentation process of the zymophyte through relatively strong pH adaptability.

The invention belongs to the technical field of agricultural biology, and discloses a method for establishing a banana explant sterile system, which comprises the following steps: taking available banana suction buds, wiping and disinfecting the surfaces with 75% alcohol, stripping banana stem tips, inoculating the banana stem tips into a screening culture medium, and carrying out dark culture; when macroscopic pyosis appears on the banana stem tips in the screening culture medium, the banana stem tips are inserted into a sterilization culture medium with bactericide liquid drops added on the surface for dark culture until the banana stem tips start to have differentiation characteristics and no pyosis appears, and the sterile explants are obtained. According to the method disclosed by the invention, the explants with pyosis are subjected to cover type sterilization and induction simultaneously after preliminary screening, so that the explants can be effectively sterilized while the differentiation of the explants is not influenced, the sterile bodies are obtained, the death probability of the explants caused by bacterial pollution is reduced to a greater extent, the success rate of subsequent conventional tissue culture is improved, and the method is suitable for large-scale popularization and application. The cultivation efficiency is improved, cost is saved, operation is easy, and popularization is facilitated.

The invention discloses a pseudo-sorghum genetic transformation method and application. The pseudo-sorghum genetic transformation method comprises the steps of pseudo-sorghum mature seed explant pretreatment, induction culture, gene gun transformation, screening culture, differentiation culture, budding culture, rooting culture and the like. According to the method disclosed by the invention, the callus is successfully induced from the pseudo-sorghum seed immature embryo, the inductivity is high, a simple and efficient genetic transformation method is established, target editing of a pseudo-sorghum gene editing plant can be realized, and the transformation efficiency and the regeneration capacity are high.

The invention discloses a screening culture medium and a preparation method and application thereof, the screening culture medium comprises a basic culture medium and additives, and the additives comprise amphotericin B, vancomycin, ox bile salt, sodium deoxycholate and No.3 bile salt. According to the screening culture medium provided by the invention, a special infectious microbe inhibitor is added on the basis of a trisaccharide iron culture medium, so that most gram-negative enterobacter, staphylococcus aureus and part of fungi except salmonella enteritis subspecies and shigella cannot grow; the interference of the infectious microbes on the detection of the salmonella enteritis subspecies and the shigella is avoided, so that the fluorescent probe can be applied to the detection of the salmonella enteritis subspecies and the shigella in a clinical anus swab, and the sensitivity of color development is improved.

A yeast dual-expression vector and a construction method therefor, a protein-protein interaction identification method and the use. The yeast dual-expression vector allows two exogenous genes to be cloned into the same vector and expressed simultaneously in yeast, thereby enabling interaction identification. The present invention greatly reduces the procedures in original yeast two-hybrid systems, such as plasmid construction and extraction, library preparation, yeast mating and screening culture, and significantly improves the identification efficiency of protein-protein interaction.

The invention relates to the technical field of plant tissue culture and genetic transformation, and particularly discloses a stable genetic transformation method of lily, which comprises the following steps: infecting a transformation donor by taking a scale of a lily seedball as a transformation receptor, co-culturing after infection, and cutting the co-cultured scale to a detoxification culture medium containing cephalosporin for detoxification to obtain the stable genetic transformation of the lily. Then inoculating to a first screening culture medium containing kanamycin and cephalosporin for screening culture to obtain a resistant seedling, inoculating the resistant seedling to a second screening culture medium containing kanamycin for screening culture, and planting the resistant seedling in a culture medium for culture to obtain a resistant plant. According to the method, the scales of the lily bulbs are directly used as transformation receptors, agrobacterium tumefaciens infection is performed, detoxification and screening culture are performed respectively to obtain stable genetic transformation resistant plants of the Lanzhou lily, sterile seedling culture is not needed, the transformation receptors are sufficient and easy to obtain, the operation process is simple and convenient, no pollution is caused, transgenic identification is easy, and false positive is low; the whole genetic transformation period is shortened.

The invention provides a construction method and application of a schizophrenia phenotype zebrafish model, and relates to the technical field of genetic engineering.According to the construction method, RNP mixed liquid containing sgRNA and Cas9 protein is introduced into zebrafish fertilized eggs, screening culture is conducted, knockout of a zebrafish pccb gene is creatively achieved, and the schizophrenia phenotype zebrafish model is obtained. The zebrafish model constructed by the invention has the advantages of high detection flux, short detection period, high detection sensitivity and few ethical limitations on detection carriers when being used for researching medicines related to mental diseases, and has wide application value for rapidly screening and evaluating schizophrenia treatment medicines and natural efficacy factors.

The application discloses a jujube genetic transformation method using anther-induced callus as a receptor, comprising the following steps: culturing sterile anthers to obtain callus; constructing a plant recombinant overexpression vector containing a target gene and transferring the plant recombinant overexpression vector into agrobacterium to obtain an agrobacterium immersion liquid; immersing the callus in the agrobacterium immersion liquid, and then co-culturing the immersed callus under dark conditions; and then placing the callus on a culture medium containing hygromycin for screening, so that a homozygous resistant callus can be obtained after 20-30 days of screening culture. The application firstly uses the anther-induced callus of Jing 39 jujube as a receptor to establish a method of the jujube genetic transformation system, the method has a high transformation frequency, and meanwhile, the method can be assisted by 35S::EGFP fluorescent reporter gene marking for screening, and is convenient and fast. The method provides convenience for jujube gene function analysis and jujube genetic improvement.