35 results about "Pingyangmycin" patented technology

The invention discloses a sodium alginate microballoon vein suppository containing hemangioma-resisting medicant, a preparation method and an application thereof. The sodium alginate microballoon vein suppository comprises carrier sodium alginate and hemangioma-resisting medicant; the sodium alginate covers the hemangioma-resisting medicant; the weight ratio of the sodium alginate to the hemangioma-resisting medicant is 5:1-60:1; and the hemangioma-resisting medicant is pingyangmycin, sodium morrhuate, carbamide or bleocin. The raw materials used in the invention have favorable mechanical strength, biocompatibility, biological degradability and stability, have no organic solvent, and are basically harmless to functions of hemopoietic system and immune system, thereby suppository can be industrially produced.

The invention provides an external traditional Chinese medicinal composition for treating xerophthalmia and a preparation method thereof. The external traditional Chinese medicinal composition for treating the xerophthalmia is prepared from the following raw materials: wolfberry, chrysanthemum, salviae miltiorrhizae, cortex phellodendri, pearl powder, borneol and menthol. The external traditional Chinese medicinal composition for treating the xerophthalmia has the advantages that pathological changes of lacrimal gland tissues of an experimental xerophthalmia mouse, lacrimal glands of which are damaged by injected pingyangmycin, in morphology can be obviously alleviated, quantity of tear fluid can be obviously increased, breakup time of a tear film is prolonged, and quantity of the tear fluid secreted by a normal domestic rabbit can be obviously increased.

The invention provides a pharmaceutical composition for treating embolism and a preparation method thereof, the pharmaceutical composition comprises a hydroxyl-contained biocompatible polymer material and a monomer containing unsaturated double bonds and anion groups, as well as a polymer generated by polymerization reaction of an optional vinyl monomer, wherein the polymerization is initiated through free radicles, and bleomycin or pingyangmycin is combined on the anion groups of the generated polymer. The preparation method combines the bleomycin or the pingyangmycin on a carrier of the polymer, thereby being capable of fully playing the dual effects of an anti-tumor antibiotic and a hardening agent owned by the bleomycin or the pingyangmycin during the embolism treatment. The anion part of the polymer can be properly combined with the bleomycin or the pingyangmycin which is rich in amino groups, thereby not only realizing the higher drug loading, but also leading the drug in an emboliaztion agent to be exchanged by cation in human body, and further realizing the slow release. In addition, the embolic carrier of the polymer has the advantages of simple prepration technology andlow cost, thereby being applicable to large-scale industrial production.

Provided is a preparation method for porous slow-release microspheres of chitosan grafting poly(N-isopropyl acrylamide / acrylamide). The method includes: first taking chitosan as a base material, adding a terminated polyethylene glycol porogenic agent, forming chitosan porous microspheres by inverse suspension dispersion and chemical cross-linking solidification, and conducting further graft copolymerizing with N-isopropyl acrylamide / acrylamide by taking the porous microspheres as substrates to obtain chitosan grafting poly(N-isopropyl acrylamide / acrylamide) porous slow-release microspheres. The microspheres have temperature sensitivity and a porous characteristic, and are uniform in pore distribution, good in dispersibility, controllable in particle size, and mild in preparation process. The microspheres can be used for embedding drug pingyangmiein. Using a microsphere dosage form helps to improve drug curative effects and reduce toxic and side effects.

The invention relates to a method for acquiring a red potato mutant and a mutagenesis medium used by the method and belongs to the technical field of agriculture Biotechnology. The mutagenesis medium is obtained by use of 30-40mg / L of pingyangmycin in a regenerated medium. The method comprises the following steps of putting purple potato square pieces into the mutagenesis medium, carrying out culture for 15 days, transferring the purple potato square pieces to a regenerated medium, and carrying out culture under culture conditions of a temperature of 22+ / -2 DEG C, illumination intensity of 700lx, illumination time of 8h / d and relative humidity of 65-75%. Through the mutagenesis medium and method, after mutagenesis for 15 days, the purple potato can produce mutation and through further culture, a potato regeneration seedling which can produce red stem tubers is obtained.

The invention discloses an application of pingyangmycin combined with sodium hyaluronate in a drug for treating lymphatic malformation, as well as a drug for treating lymphatic malformation, in particular to a drug for treating microcystic lymphatic malformation. According to the application, the pingyangmycin combined with the sodium hyaluronate is adopted and has better effects in the aspects of inhibiting human lymphatic malformation endothelial cell proliferation and promoting apoptosis while compared with pure pingyangmycin, wherein the molecular weight of the sodium hyaluronate is 600,000 Dalton-1,500,000 Dalton, and concentrations of the pingyangmycin and the sodium hyaluronate are 100 mu g / mL and 300 mu g / mL respectively.

The invention provides a new application of a traditional Chinese medicinal composition and in particular relates to an application of a traditional Chinese medicinal composition in preparation of a medicine used for treating xerophthalmia, and raw materials of the traditional Chinese medicinal composition comprise wolfberry, chrysanthemum, salviae miltiorrhizae, cortex phellodendri, borneol, pearl powder and menthol. The traditional Chinese medicinal composition has the advantages that pathological changes of lacrimal gland tissues of an experimental xerophthalmia mouse, lacrimal glands of which are damaged by injected pingyangmycin, in morphology can be obviously alleviated, quantity of tear fluid can be obviously increased, breakup time of a tear film is prolonged, and quantity of the tear fluid secreted by a normal domestic rabbit can be obviously increased; and meanwhile, the traditional Chinese medicinal composition has obvious anti-inflammation and anti-oxidation effects.

The invention provides a method for directionally screening a salt-tolerant body through peanut in vitro mutagenesis. The method mainly comprises the following steps: sterilizing the surface of peanut embryo; separating a leaflet, inoculating into a somatic embryo induction and mutagenesis medium added with 2,4-D and pingyangmycin, and performing mutagenesis culture treatment; and transferring a survival explant on which a somatic embryo is formed to a somatic embryo germination and screening culture medium added with bone alkaline phosphatase (BAP) and NaCl, and directionally screening the salt-tolerant body. The salt-tolerant body is directionally screened by combining in vitro mutagenesis and tissue culture, and plenty of manpower, materials and financial resources can be saved; and the salt-tolerant body is regenerated in an embryogenesis way, an somatic embryo is derived from a cell, and the chimerism of the salt-tolerant body can be avoided. A peanut mature seed is taken as a mutagenic material and cannot be limited by seasons, the operation is convenient, a novel salt-tolerant peanut germplasm can be created, the peanut genetic basis is enriched, and the difficulty that new high-yield salt-tolerant varieties are difficult to breed due to the lack of high salt-tolerant germplasm resources in peanut cultispecies is overcome.

The invention discloses a pingyangmycin polyethylene glycol (PEG)-polycaprolactone (PCL)-polyethylene glycol (PEG) temperature-sensitive slow-release gel, as well as a preparation method and the application of the gel. The Pingyangmycin PEG-PCL-PEG temperature-sensitive slow-release gel mainly consists of two parts comprising PEG (polyethylene glycol)-PCL (polycaprolactone)-PEG (polyethylene glycol) co-polymer and Pingyangmycin, is liquid at room temperature, and is solid gel under the in vivo 37 DEG C condition; the gel system has significant slow-release effect, thereby having functions in prolonging the half-life period and the acting time of the Pingyangmycin, reducing drug concentration in plasma, and reducing systemic toxic and side effects. The gel can be formed in situ after the medicine is injected in a high-flow-speed vessel, and then location embolism is realized, and so as to realize the, and muscularization and closing of muscle is caused, so that the sclerotherapy and the interventional therapy are combined effectively, the gel has excellent biocompatibility and degradability, is beneficial for treating hemangiomas, vascular malformations and cancers, particularly, a new selection is provided to the treatment of the vein malformation and partial malformation of cancers.

The invention provides a kind of chemical mutagen that is used for corn genetic breeding, described chemical mutagen is the combination of Pingyangmycin and N-methyl-N-nitrosourea; Wherein the concentration of Pingyangmycin is 20ppm, The concentration of N-methyl-N-nitrosourea is 0.2%. The chemical mutagen can be used in corn genetic breeding.

The invention discloses a fermentation medium for producing pingyangmycin and fermentation methods. The medium comprises the following components of a nitrogen source, a carbon source, inorganic salt,trace elements and water; the nitrogen source accounts for 3.55%-9.25% of the mass of the fermentation medium; the nitrogen source comprises cold-pressed soybean cake powder and a botanical drug; thebotanical drug is one or more of ningxia chinese wolfberry, ningxia chinese wolfberry tender leaves and coix seeds; and the botanical drug accounts for 0.1%-1.5% of the mass of the fermentation medium. The carbon and nitrogen sources in the fermentation medium are agricultural and sideline products, the price is low, the chemical property is stable, the sources are wide, the transportation and storage are convenient, no toxic and harmful substance residues exist in the fermentation process, the yield of the pingyangmycin is increased, the fermentation period is shortened, and the industrial production is facilitated.

The invention discloses a snake poison cytotoxin, the preparation method and the application. The method of preparing the snake poison cytotoxin in the invention includes the following steps: firstly, the snake poison is conducted an ion-exchange chromatography and then the eluent between a first absorption peak and a second absorption peak on the absorbance curve with a detection wavelength of 280nm in the eluent is gathered; secondly, the eluent obtained in the first step is conducted an exclusion chromatography, water is used as a moving phase, and the second absorption peak on the absorbance curve with a detection wavelength of 280 nm in the eluent is gathered to obtain the snake poison cytotoxin. The saw-scale viper poison cytotoxin in the invention can induce the human laryngeal cancer cell Hep-2 to an apoptosis and the capability of inducing Cell Hep-2 to an apoptosis is stronger than Pingyangmycin with a viscosity of 100 Mug / ml when the snake poison cytotoxin has a concentration of 0.01 mg per milliliter. In addition, experiments of undue toxicity prove that the cytotoxin has slight side effects. The saw-scale viper poison cytotoxin in the invention can be used as an anti-cancer drug to turn biological anti-cancer drugs into a reality.

The invention provides an external traditional Chinese medicinal composition for treating xerophthalmia and a preparation method thereof. The external traditional Chinese medicinal composition for treating the xerophthalmia is prepared from the following raw materials: wolfberry, chrysanthemum, salviae miltiorrhizae, cortex phellodendri, pearl powder, borneol and menthol. The external traditional Chinese medicinal composition for treating the xerophthalmia has the advantages that pathological changes of lacrimal gland tissues of an experimental xerophthalmia mouse, lacrimal glands of which are damaged by injected pingyangmycin, in morphology can be obviously alleviated, quantity of tear fluid can be obviously increased, breakup time of a tear film is prolonged, and quantity of the tear fluid secreted by a normal domestic rabbit can be obviously increased.

The invention discloses a method for producing pingyangmycin by fermentation. The invention discloses a method for fermenting and producing Pingyangmycin, which comprises fermenting Streptomyces verticillium in a fermentation medium, and adding exogenous precursors to the fermentation medium during the fermentation process to obtain the fermentation medium containing Pingyangmycin The product is isolated from the fermentation product to obtain Pingyangmycin; wherein, the exogenous precursor is substance A and / or substance B, and the substance A is adenosylmethionine or its similar amino acid derivatives, The substance B is spermidine or a salt of spermidine. The fermentative production method of Pingyangmycin provided by the present invention is simple and effective, can rapidly improve the fermentation production level of Pingyangmycin, reduce production cost, does not need additional equipment and manpower, can greatly improve the industrial fermentation level of Pingyangmycin, and is suitable for industrial scale production.