A method for directed selection of salt-tolerant body by in vitro mutagenesis

Abstract

The invention provides a method for directionally screening a salt-tolerant body through peanut in vitro mutagenesis. The method mainly comprises the following steps: sterilizing the surface of peanut embryo; separating a leaflet, inoculating into a somatic embryo induction and mutagenesis medium added with 2,4-D and pingyangmycin, and performing mutagenesis culture treatment; and transferring a survival explant on which a somatic embryo is formed to a somatic embryo germination and screening culture medium added with bone alkaline phosphatase (BAP) and NaCl, and directionally screening the salt-tolerant body. The salt-tolerant body is directionally screened by combining in vitro mutagenesis and tissue culture, and plenty of manpower, materials and financial resources can be saved; and the salt-tolerant body is regenerated in an embryogenesis way, an somatic embryo is derived from a cell, and the chimerism of the salt-tolerant body can be avoided. A peanut mature seed is taken as a mutagenic material and cannot be limited by seasons, the operation is convenient, a novel salt-tolerant peanut germplasm can be created, the peanut genetic basis is enriched, and the difficulty that new high-yield salt-tolerant varieties are difficult to breed due to the lack of high salt-tolerant germplasm resources in peanut cultispecies is overcome.

Description

technical field [0001] The invention relates to a method for directional screening of peanut in vitro mutagenesis, in particular to a method for directional screening of peanut in vitro mutagenesis for salt-tolerant bodies. Background technique [0002] With the continuous expansion of irrigated land and protected cultivation area, coastal tidal flats are formed due to backwashing by seawater, and saline-alkali land has a tendency to expand continuously, and the available cultivated land resources are decreasing year by year. Saline soil will affect the growth of vegetation, cause crop yield reduction or failure, and indirectly cause the deterioration of the ecological environment. [0003] Peanut is one of the important oil crops and economic crops in my country. Peanut is a drought-resistant, barren-resistant and adaptable crop. It is widely planted all over the world, but peanut is not a salt-tolerant crop. my country's per capita arable land area is small, and ensuring ...

AI Technical Summary

Problems solved by technology

[0005] The invention provides a method for directional screening of salt-tolerant peanuts by in vitro mutagenesis, which can solve the problems of narrow genetic basis of peanuts, lack of salt-tolerant variety resources, and difficulty in cultivating high-yield, salt-tolerant peanut varieties by conventional breeding

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