Method for cultivating high-oil peanut varieties

Abstract

The invention provides a method for cultivating high-oil peanut varieties. The method mainly comprises the following steps: young peanut leaves with seedling age of 5-7 days are cultivated in an induction and mutagenesis medium with NAA, BAP and pingyangmycin for in-vitro mutagenesis culture; surviving explants are transferred to an adventitious bud differentiation and high-oil body directional screening medium supplemented with BAP and hydroxyproline for culture; obtained high-oil seedlings are transplanted to plastic greenhouses or high-temperature greenhouses after aseptic grafting, and normal seeds are obtained; progeny of the obtained high-oil bodies are bred with pedigree selection, and by combination with generation increasing in Hainan island and high-temperature greenhouses, 3 generations can be planted each year. The high-oil bodies are screened by in-vitro mutagenesis and in-vitro directional screening, and the problems of low mutagenesis frequency and incapability of directional breeding in conventional mutagenesis breeding are solved. The high-oil bodies are directionally screened and non-high-oil bodies are eliminated in the medium, and the problem of consumption of alarge quantity of manpower, material resources and financial resources in the identification of mutant progeny is solved.

Description

technical field [0001] The invention belongs to the field of peanut breeding, in particular to a method for cultivating high-oil varieties of peanuts. Background technique [0002] Peanut is the second largest oil-bearing crop in my country. As an oil-use variety, every 1 percentage point increase in oil content can increase net profit by 7%. Therefore, the majority of peanut breeders regard cultivating new high-oil peanut varieties as one of the main breeding goals. However, due to poor genetic diversity and lack of high-oil germplasm resources, it is difficult to obtain high-oil offspring through cross-breeding. Therefore, the selection of high-yield and high-oil varieties has always been a difficult problem to solve. The use of mutagenesis technology can cause gene mutation or chromosomal variation, so as to obtain new traits and new types that do not exist in nature or are extremely rare. However, the identification of mutant offspring requires a lot of manpower, materi...

AI Technical Summary

Problems solved by technology

[0004] The purpose of the present invention is to overcome the deficiencies of the above-mentioned prior art and provide a method for cultivating high-oil varieties of peanuts, which can solve the problem that it is difficult to cultivate high-yield and high-oil varieties of peanuts in conventional breeding.

Images