126 results about "Oil body" patented technology

The present invention provides a foamable composition for administration to the skin, body surface, body cavity or mucosal surface, e.g., the mucosa of the nose, mouth, eye, ear, respiratory system, vagina or rectum. The foamable oil in water emulsion composition includes: an oil globule system, selected from the group consisting of oil bodies; and sub-micron oil globules, about 0.1% to about 5% by weight of an agent, selected from the group consisting of a surface-active agent, having an HLB value between 9 and 16; and a polymeric agent, and a liquefied or compressed gas propellant at a concentration of about 3% to about 25% by weight of the total composition, water and optional ingredients are added to complete the total mass to 100%. Upon release from an aerosol container, the foamable composition forms and expanded foam suitable for topical administration.

Skin care and cleansing compositions are described that contain oil seed products that provide moisturization and in a preferred embodiment simultaneous exfoliation. Oil seed products contain oil bodies, seed shell and optionally other seed components. In a further embodiment, a toilet bar is provided that contains one or more anionic surfactants and 0.1 to 5.0% by wt. of oil seed product particles where a majority of the particles have a major axis length of between 0.01 to 200 microns.

The invention provides emulsified oil for a hydraulic support for mines and a preparation method. The emulsified oil comprises the components in percentage by weight as follows: 5%-15% of mineral base oil and 85%-95% of an addition agent; and the addition agent is prepared by mixing the component in parts by weight as follows: 5-15 parts of oleic acid, 6-12 parts of triethanolamine, 5-10 parts of castor oil soda soap, 0.3-0.5 part of benzotriazole, 5-12 parts of EDTA (ethylene diamine tetraacetic acid) disodium, 5-7 parts of monoethanolamine, 5-8 parts of urea, 30-50 parts of water, 5-8 parts of POEA-15, 6-15 parts of a water base antirust agent and 0.5-0.8 part of silicone emulsion. The emulsified oil has good appearance, transparent oil color and no smell and volatile substance; and the inner environment of the oil body can be easily observed, so that the worker bodies are prevented from being damaged, the environment is prevented from being destroyed, and the emulsified oil is green and environment-friendly.

An oil body carrier, its uses in target therapy and/or detection, and a fusion protein comprised therein are provided. The oil body carrier comprises:

a) the fusion protein, comprising an oil body protein and a ligand peptide, an antibody peptide, a cell penetrating peptide, or combinations thereof, and

b) a lipid,

wherein the weight/volume (μg/μl) ratio of the fusion protein and the lipid is at least about 1/25, and the average particle size of the oil body carrier is about 10 nm to about 2,000 nm.

The invention discloses an extraction method of a soybean oil body having high oxidation resistance, and belongs to the field of health food or biotechnology. The soybean oil body having high oxidation resistance is extracted from soybean seeds through such processes as soaking, grinding, filtering, centrifuging and homogenizing. According to the extraction method disclosed by the invention, the oil body is extracted by taking water as a medium, so that the method is non-toxic, and simple and convenient to operate, and the soybean oil body is stable, relatively high in purity and conducive to the application in food. The oil body extracted by virtue of the method can be dried or rehydrated and added to any other foods or animal feed products such as peanut butter.

The invention discloses a method for extracting and preparing algae oil DHA, which comprises the following steps: (1) cultivating and fermenting Cryptidium koii; (2) drying; (3) pretreatment by breaking the wall; (4) microalgae DHA extraction; (5) urea inclusion enrichment and purification; the microalgae oil obtained in step (4) is enriched and purified by urea inclusion method to purify DHA; obtain microalgae DHA algae oil; (6) decolorization and deodorization; urea inclusion The microalgal DHA algae oil obtained by enrichment and purification is decolorized by adding activated clay accounting for 3% of the oil body mass. The decolorization temperature is 80°C and the decolorization time is 45 minutes. Steam stripping deodorization, the stripping temperature is 85°C, the vacuum degree is 0.30Kpa, the deodorization time is 1 hour, then slowly cool under the vacuum state, and centrifuge to obtain the algae oil DHA product.

The invention provides modified oleosins, including at least one artificially introduced cysteine, and methods and compositions for producing the modified oleosins. Also provided are polynucleotides encoding the modified oleosins, constructs and host cells comprising the polynucleotides, methods for producing oil bodies comprising the modified oleosins, in vivo and in vitro, and methods for producing oil in host cells and plants. The invention also provides methods for increasing the rate of CO₂ assimilation in photosynthetic cells and plants, and involves reducing or preventing lipid recycling, and/or expressing modified oleosins with artificially introduced cysteine residues in the photosynthetic cells and plants. Also provided are methods for increasing oil production in plants, via expression of modified oleosins in the non-photosynthetic tissues/organs of plants. The method also optionally includes the step of extrating the oil from the non-photosynthetic tissues/organs of the plant, or processing the oil rich non-photosynthetic tissues/organs into animal or biofuel feedstocks.

The invention discloses an artificial oil body carrier for the targeted therapy of mastocarcinoma as well as a preparation method and an application thereof. With the preparation method, a pJO1-oleZH2 plasmid is converted to enter the host cell of escherichia coli BL21 (DE3), oleZH2 proteins are produced by induction, and oil and the oleZH2 proteins are mixed to prepare an artificial oil body carrier. Invitro and invivo experiments confirm that the artificial oil body carrier disclosed by the invention can be effectively applied to target therapy, and the oil body carried covered with fluorescent substances can reflect HER2/neu positive cancer cells so as to specifically detect the mastocarcinoma cell excessively expressing HER2/neu. The artificial oil body carrier has the advantages of simple preparation process, extra small environmental pollution, good stability in blood cells or serum, small volume and the like, is cheap and efficient, is convenient to operate and is easy to transport. The artificial oil body carrier can be used for covering anti-mastocarcinoma drugs of different types of oil solubility, can effectively kill mastocarcinoma cells after being coated on drugs and can carry out specific detection and target therapy for mastocarcinoma cells.

The present invention relates to artificial oil bodies comprising oleosin (which, as presently defined, also encompasses caleosin, steroleosin and polyoleosin), a surfactant such as a phospholipid, and an oil comprising fatty acids, such as polyunsaturated fatty acids having four or more double bonds. The present invention also relates to methods of preparing said artificial oil bodies. These AOBs may further comprise other molecules such as bioactive molecules, used in a wide variety of products, and are particularly useful for producing oxidatively stable oil-in-water emulsions in the absence of added antioxidants. The present invention further encompasses a method for the partial purification of oleosin from a plant extract.

The invention provides a method of expressing human insulin humulin by plant seed oil body. The invention designs human insulin humulin genes according to plant codon bias, mixing the human insulin humulin genes and oil body protein gene to construct expression carrier containing the mixing genes, further conversing the expression to receptor plant to get conversing palnt whose seeds can effectively express human insulin humulin. The invention uses plant bio reactor to produce human insulin humulin avoiding harm of animal pathogen and endotoxin of coliform bacteria, and can simplify departing and purification process of goal proteins to reduce cost and good for industrialization of human insulin humulin.

The present invention relates to the use of oil bodies as a vaccine adjuvant and delivery system for administration of vaccines by parenteral, mucosal (oral, nasal, pulmonary) and transdermal routes. In addition, the present invention relates to methods of eliciting an immune response in an animal by administering oil body-antigen complexes to said mammal. Finally, the present invention relates to methods of preparing oil body-antigen complexes.

The present invention describes novel polypeptide structures based on oleosin molecules which are capable of being targeted to oil bodies in plants. The modified oleosin polypeptides are obtained by performing modifications in the hydrophobic domain encoding sequence of an oleosin cDNA. The present invention describes methods to obtain such polypeptides in vivo. The novel oleosins may be used to deliver a recombinant (non-oleosin) protein to oil bodies.

The invention discloses application of electrically polymerizing a water and oil body through bio-based ionic liquid. Ionic liquid is mainly prepared from plant acid and plant alkaline through one-step reaction; ionic liquid formed by an action between acid and alkaline has very high affinity with water; meanwhile, a hydrophobic group is introduced, so that the ionic liquid further generates interaction with hydrophobic molecules (an oil phase); and the ionic liquid supplements with the hydrophobic molecules, so that the ionic liquid can fuse a water phase and the oil phase; the ionic liquid is stirred for 20-60 minutes under an electric polymerization condition of 20 kHz-40kHz, so that the water and oil body is fused more uniformly, the use of chemical emulsifiers is also avoided, and thewater and oil body can be fused by the method safer. The method is beneficial for preparing substrate materials of safe, natural and gentle daily chemical products; and the formed fused molecular groups are small and are regularly arranged, so that hydrophilic-lipophilic properties are better, and transdermal penetration is realized easier.

The present invention provides a method of extracting naturally occurring oil bodies comprising obtaining material containing naturally occurring oil bodies,recovering the oil bodies in a wet preparation and drying the oil bodies; and dried oil bodies obtained by the method and uses thereof.

The invention provides a method for simultaneously preparing an oil body and non-hydrolyzed protein, comprising the following steps: (i) providing an oil material; (ii) in existence of water and enzyme, destroying the cell walls of the oil material and/or destroying cellulose, pectin, hemicellulose or glucan in the cell walls of the oil material; and (iii) separating the oil body and protein obtained by reaction.

The present invention is gene recombination process, in which human epidermal growth factor, (hEGF) and oil-body protein gene are fused to constitute one plant oil-body expressing vector. Root nodule agrobacterium is used to transform Arabidopsis thalianuum, safflower, soybean, black bean and rape to establish genetic expressing system of fusion expressed oil-body protein gene and the target gene and to obtain transgenic plant. The molecular biology detection on transgenic safflower and rape proves the integration and expression of exogenous gene, and the activity detection proves the bioactivity corresponding to the standard hEGF activity of obtained recombinant protein. The present invention also provides the method of expressing hEGF in plant seed, and the method may be used in producing hEGF at low cost and developing new medicine.

The invention provides a method for mining oil. The method comprises the steps that horizontal well groups are set up according to an oil storage layer; steam is injected into the horizontal well groups to enable the oil storage layer to form a steam cavity; a vertical well is set up according to the oil storage layer; and carbon dioxide is injected into the vertical well to enable an oil body inthe oil storage layer to be discharged by the horizontal well groups. According to the method for mining oil, the output of the oil can be effectively increased.

A method of enzyme-based processing of plant-based materials includes steps of providing a bulk amount of a plant-based material having a substantial amount of the cell walls in a physically-intact condition; providing an enzyme broth having an enzyme capable of breaking down the cell walls in the physically-intact condition; combining the bulk amount of the plant-based material with the enzyme broth; allowing the enzyme capable of breaking down the cell walls in the physically-intact condition to break down at least a portion of the cell walls in the physically-intact condition to thereby produce intact oil bodies, hydrolyzed carbohydrates, and intact protein bodies; collecting a first product including the intact oil bodies; collecting a second product including the hydrolyzed carbohydrates; and collecting a third product including the intact protein bodies.

The invention discloses a vegetable oil constant-temperature squeezer. A squeezing mechanism comprises a hydraulic telescopic rod, a movable mounting seat fixedly connected to the hydraulic telescopicrod, a squeezing barrel fixedly connected to the movable mounting seat, an oil receiving barrel fixedly connected to the movable mounting seat and arranged around the squeezing barrel, a lower-layersqueezing block mounted in the squeezing barrel, and an upper-layer squeezing block opposite to the lower-layer squeezing block. A raw material constant-temperature heating mechanism is arranged at the height position between the upper-layer squeezing block and the squeezing barrel and comprises a sliding mounting plate sliding horizontally, a heating kettle fixed to the sliding mounting plate, aheating oil pipe arranged on the inner wall of the heating kettle, and a stirring device in the heating kettle. The bottom of the oil receiving barrel communicates with an inlet of the heating oil pipe through a communicating hose. The vegetable oil constant-temperature squeezer has the advantages that the oil squeezing efficiency is high, it can be guaranteed that vegetable oil squeezing raw materials are subjected to oil squeezing treatment at the constant temperature, and waste heat of produced oil is utilized conveniently.

The invention discloses a preparation method of freeze-dried oil body. The preparation method includes the steps of: 1) preparing an oil body PBS emulsion and a mannitol PBS solution respectively from a PBS solution containing 0.03-0.05 wt% of methylparaben and 0.03-0.05 wt% of propylparaben and being 8.0-8.5 in pH value; 2) adding the two solutions hereinabove into a container at equal volume in a parallel flow manner with stirring to obtain an oil body/mannitol PBS emulsion, wherein the final concentration of the oil body is 0.05-0.20 g/ml and the final concentration of mannitol is 0.8-3 times of that of the oil body; and 3) pre-freezing the mixture, and freeze-drying the mixture in a freeze-drying machine. The oil body freeze-dried powder, after six months at room temperature, has uniform appearance particle size, has no significant color change, and is easy to re-dissolve and hydrate. Microscope structure detection after hydration proves that the oil body is free of large oil drops, is homogenous, and has no bacterial colony after bacterial detection. The method solves a problem that the oil body is difficult to store. The oil body is convenient to store and transport, is reduced in the content of mannitol, and provides basis for utilizing the oil body to produce foods, cosmetics and medicines better.