134 results about "Oil body" patented technology

The present invention provides a foamable composition for administration to the skin, body surface, body cavity or mucosal surface, e.g., the mucosa of the nose, mouth, eye, ear, respiratory system, vagina or rectum. The foamable oil in water emulsion composition includes: an oil globule system, selected from the group consisting of oil bodies; and sub-micron oil globules, about 0.1% to about 5% by weight of an agent, selected from the group consisting of a surface-active agent, having an HLB value between 9 and 16; and a polymeric agent, and a liquefied or compressed gas propellant at a concentration of about 3% to about 25% by weight of the total composition, water and optional ingredients are added to complete the total mass to 100%. Upon release from an aerosol container, the foamable composition forms and expanded foam suitable for topical administration.

Skin care and cleansing compositions are described that contain oil seed products that provide moisturization and in a preferred embodiment simultaneous exfoliation. Oil seed products contain oil bodies, seed shell and optionally other seed components. In a further embodiment, a toilet bar is provided that contains one or more anionic surfactants and 0.1 to 5.0% by wt. of oil seed product particles where a majority of the particles have a major axis length of between 0.01 to 200 microns.

The present invention provides novel emulsions that comprise oil bodies. The invention also relates to novel methods for generating formulations comprising oil bodies and active ingredients wherein the active ingredient is partitioned into the oil body. The methods are particularly useful for generating emulsions with either hydrophobic or amphipathic biologically active agents.

The present invention relates to the use of a class of genes called oil body protein genes that have unique features. The discovery of these features allowed the invention of methods for the production of recombinant proteins wherein a protein of interest can be easily separated from other host cell components. The invention is further exemplified by methods for exploitation of the unique characteristics of the oil body proteins and oil body genes for expression of polypeptides of interest in many organisms, particularly plant seeds. Said polypeptides may include but are not limited to: seed storage proteins, enzymes, bioactive peptides, antibodies and the like. The invention can also be modified to recover recombinant polypeptides fused to oil body proteins from non-plant host cells. Additionally the invention provides a method of using recombinant proteins associated with seed oil bodies released during seed germination for expression of polypeptides that afford protection to seedlings from pathogens. Finally, the persistent association of oil body proteins with the oil body can be further utilized to develop a biological means to create novel immobilized enzymes useful for bioconversion of substrates.

The invention provides emulsified oil for a hydraulic support for mines and a preparation method. The emulsified oil comprises the components in percentage by weight as follows: 5%-15% of mineral base oil and 85%-95% of an addition agent; and the addition agent is prepared by mixing the component in parts by weight as follows: 5-15 parts of oleic acid, 6-12 parts of triethanolamine, 5-10 parts of castor oil soda soap, 0.3-0.5 part of benzotriazole, 5-12 parts of EDTA (ethylene diamine tetraacetic acid) disodium, 5-7 parts of monoethanolamine, 5-8 parts of urea, 30-50 parts of water, 5-8 parts of POEA-15, 6-15 parts of a water base antirust agent and 0.5-0.8 part of silicone emulsion. The emulsified oil has good appearance, transparent oil color and no smell and volatile substance; and the inner environment of the oil body can be easily observed, so that the worker bodies are prevented from being damaged, the environment is prevented from being destroyed, and the emulsified oil is green and environment-friendly.

The present invention provides novel emulsion formulations containing oil bodies. The invention also provides a process for the preparation of the emulsion and the use of the emulsion in cosmetic and industrial compositions. Emulsions are particularly suitable for the preparation of food, personal care, pharmaceutical and industrial products.

An oil body carrier, its uses in target therapy and / or detection, and a fusion protein comprised therein are provided. The oil body carrier comprises:a) the fusion protein, comprising an oil body protein and a ligand peptide, an antibody peptide, a cell penetrating peptide, or combinations thereof, andb) a lipid,wherein the weight / volume (μg / μl) ratio of the fusion protein and the lipid is at least about 1 / 25, and the average particle size of the oil body carrier is about 10 nm to about 2,000 nm.

The invention discloses an extraction method of a soybean oil body having high oxidation resistance, and belongs to the field of health food or biotechnology. The soybean oil body having high oxidation resistance is extracted from soybean seeds through such processes as soaking, grinding, filtering, centrifuging and homogenizing. According to the extraction method disclosed by the invention, the oil body is extracted by taking water as a medium, so that the method is non-toxic, and simple and convenient to operate, and the soybean oil body is stable, relatively high in purity and conducive to the application in food. The oil body extracted by virtue of the method can be dried or rehydrated and added to any other foods or animal feed products such as peanut butter.

The invention discloses a method for extracting and preparing algae oil DHA, which comprises the following steps: (1) cultivating and fermenting Cryptidium koii; (2) drying; (3) pretreatment by breaking the wall; (4) microalgae DHA extraction; (5) urea inclusion enrichment and purification; the microalgae oil obtained in step (4) is enriched and purified by urea inclusion method to purify DHA; obtain microalgae DHA algae oil; (6) decolorization and deodorization; urea inclusion The microalgal DHA algae oil obtained by enrichment and purification is decolorized by adding activated clay accounting for 3% of the oil body mass. The decolorization temperature is 80°C and the decolorization time is 45 minutes. Steam stripping deodorization, the stripping temperature is 85°C, the vacuum degree is 0.30Kpa, the deodorization time is 1 hour, then slowly cool under the vacuum state, and centrifuge to obtain the algae oil DHA product.

The invention provides modified oleosins, including at least one artificially introduced cysteine, and methods and compositions for producing the modified oleosins. Also provided are polynucleotides encoding the modified oleosins, constructs and host cells comprising the polynucleotides, methods for producing oil bodies comprising the modified oleosins, in vivo and in vitro, and methods for producing oil in host cells and plants. The invention also provides methods for increasing the rate of CO2 assimilation in photosynthetic cells and plants, and involves reducing or preventing lipid recycling, and / or expressing modified oleosins with artificially introduced cysteine residues in the photosynthetic cells and plants. Also provided are methods for increasing oil production in plants, via expression of modified oleosins in the non-photosynthetic tissues / organs of plants. The method also optionally includes the step of extrating the oil from the non-photosynthetic tissues / organs of the plant, or processing the oil rich non-photosynthetic tissues / organs into animal or biofuel feedstocks.

The invention discloses an artificial oil body carrier for the targeted therapy of mastocarcinoma as well as a preparation method and an application thereof. With the preparation method, a pJO1-oleZH2 plasmid is converted to enter the host cell of escherichia coli BL21 (DE3), oleZH2 proteins are produced by induction, and oil and the oleZH2 proteins are mixed to prepare an artificial oil body carrier. Invitro and invivo experiments confirm that the artificial oil body carrier disclosed by the invention can be effectively applied to target therapy, and the oil body carried covered with fluorescent substances can reflect HER2 / neu positive cancer cells so as to specifically detect the mastocarcinoma cell excessively expressing HER2 / neu. The artificial oil body carrier has the advantages of simple preparation process, extra small environmental pollution, good stability in blood cells or serum, small volume and the like, is cheap and efficient, is convenient to operate and is easy to transport. The artificial oil body carrier can be used for covering anti-mastocarcinoma drugs of different types of oil solubility, can effectively kill mastocarcinoma cells after being coated on drugs and can carry out specific detection and target therapy for mastocarcinoma cells.

The present invention relates to artificial oil bodies comprising oleosin (which, as presently defined, also encompasses caleosin, steroleosin and polyoleosin), a surfactant such as a phospholipid, and an oil comprising fatty acids, such as polyunsaturated fatty acids having four or more double bonds. The present invention also relates to methods of preparing said artificial oil bodies. These AOBs may further comprise other molecules such as bioactive molecules, used in a wide variety of products, and are particularly useful for producing oxidatively stable oil-in-water emulsions in the absence of added antioxidants. The present invention further encompasses a method for the partial purification of oleosin from a plant extract.

This invention provides a method for expressing human metallothionein (MT) by using plant oil. The method comprises: designing and synthesizing human MT gene according to the preference of plant codon, fusing human MT gene with plant oil protein gene, constructing an expression vector with the fused genes, converting recipient plant by using the expression vector to obtain converted plant, and efficiently expressing human MT by using the seed of the converted plant. Activity experiments show that purified recombinant MT has comparable activity with human origin MT. The method utilizes plant bioreactor to produce MT, thus can avoid possible attack by animal pathogen and toxin in E. coli. The method can largely simplify the separation and purification process of human MT, and lower the production cost, this is advantageous for MT industrialization.

The invention provides a method of expressing human insulin humulin by plant seed oil body. The invention designs human insulin humulin genes according to plant codon bias, mixing the human insulin humulin genes and oil body protein gene to construct expression carrier containing the mixing genes, further conversing the expression to receptor plant to get conversing palnt whose seeds can effectively express human insulin humulin. The invention uses plant bio reactor to produce human insulin humulin avoiding harm of animal pathogen and endotoxin of coliform bacteria, and can simplify departing and purification process of goal proteins to reduce cost and good for industrialization of human insulin humulin.

The present invention relates to the use of oil bodies as a vaccine adjuvant and delivery system for administration of vaccines by parenteral, mucosal (oral, nasal, pulmonary) and transdermal routes. In addition, the present invention relates to methods of eliciting an immune response in an animal by administering oil body-antigen complexes to said mammal. Finally, the present invention relates to methods of preparing oil body-antigen complexes.

The present invention describes novel polypeptide structures based on oleosin molecules which are capable of being targeted to oil bodies in plants. The modified oleosin polypeptides are obtained by performing modifications in the hydrophobic domain encoding sequence of an oleosin cDNA. The present invention describes methods to obtain such polypeptides in vivo. The novel oleosins may be used to deliver a recombinant (non-oleosin) protein to oil bodies.

Improved methods for the production of multimeric-protein-complexes, such as redox proteins and immunoglobulins, in association with oil bodies are described. The redox protein is enzymatically active when prepared in association with the oil bodies. Also provided are related nucleic acids, proteins, cells, plants, and compositions.

Compositions and methods for reducing hypercholesterolemia and, accordingly, the risk of cardiovascular disease, are provided. Such compositions may comprise isolated oil body associated proteins. Additionally provided are foodstuffs to which one or more oil body associated proteins have been added. The compositions employed in the invention may further comprise additive compounds, for example, a saponin, an isoflavone, a phospholipid, a carbohydrate substantially resistant to digestion, or a combination thereof. The methods and compositions of the invention may be used to lower cholesterol and other lipid levels in subjects to achieve a reduction in the risk of cardiovascular disease.

The invention discloses application of electrically polymerizing a water and oil body through bio-based ionic liquid. Ionic liquid is mainly prepared from plant acid and plant alkaline through one-step reaction; ionic liquid formed by an action between acid and alkaline has very high affinity with water; meanwhile, a hydrophobic group is introduced, so that the ionic liquid further generates interaction with hydrophobic molecules (an oil phase); and the ionic liquid supplements with the hydrophobic molecules, so that the ionic liquid can fuse a water phase and the oil phase; the ionic liquid is stirred for 20-60 minutes under an electric polymerization condition of 20 kHz-40kHz, so that the water and oil body is fused more uniformly, the use of chemical emulsifiers is also avoided, and thewater and oil body can be fused by the method safer. The method is beneficial for preparing substrate materials of safe, natural and gentle daily chemical products; and the formed fused molecular groups are small and are regularly arranged, so that hydrophilic-lipophilic properties are better, and transdermal penetration is realized easier.

The present invention provides a method of extracting naturally occurring oil bodies comprising obtaining material containing naturally occurring oil bodies,recovering the oil bodies in a wet preparation and drying the oil bodies; and dried oil bodies obtained by the method and uses thereof.

The invention provides a method for simultaneously preparing an oil body and non-hydrolyzed protein, comprising the following steps: (i) providing an oil material; (ii) in existence of water and enzyme, destroying the cell walls of the oil material and / or destroying cellulose, pectin, hemicellulose or glucan in the cell walls of the oil material; and (iii) separating the oil body and protein obtained by reaction.

The present invention relates to methods of producing industrial products from plant lipids, particularly from vegetative parts of plants. In particular, the present invention provides oil products such as biodiesel and synthetic diesel and processes for producing these, as well as plants having an increased level of one or more non-polar lipids such as triacylglycerols and an increased total non-polar lipid content. In one particular embodiment, the present invention relates to combinations of modifications in two or more of lipid handling enzymes, oil body proteins, decreased lipid catabolic enzymes and / or transcription factors regulating lipid biosynthesis to increase the level of one or more non-polar lipids and / or the total non-polar lipid content and / or mono-unsaturated fatty acid content in plants or any part thereof. In an embodiment, the present invention relates to a process for extracting lipids. In another embodiment, the lipid is converted to one or more hydrocarbon products in harvested plant vegetative parts to produce alkyl esters of the fatty acids which are suitable for use as a renewable biodiesel fuel.

The present invention is gene recombination process, in which human epidermal growth factor, (hEGF) and oil-body protein gene are fused to constitute one plant oil-body expressing vector. Root nodule agrobacterium is used to transform Arabidopsis thalianuum, safflower, soybean, black bean and rape to establish genetic expressing system of fusion expressed oil-body protein gene and the target gene and to obtain transgenic plant. The molecular biology detection on transgenic safflower and rape proves the integration and expression of exogenous gene, and the activity detection proves the bioactivity corresponding to the standard hEGF activity of obtained recombinant protein. The present invention also provides the method of expressing hEGF in plant seed, and the method may be used in producing hEGF at low cost and developing new medicine.

Belonging to the technical field of genetic engineering, the invention provides a method for producing human insulin-like growth factor-1 (human insulin-like growth factor 1, hIGF-1) by the vegetable oil ribosomal protein expression system. In the method of the invention, human insulin-like growth factor-1 gene is designed and synthesized according to preference of vegetable codon and the human insulin-like growth factor-1 gene is fused with oil body protein gene, thus constructing a vegetable expression vector which expresses promoter drive by seed specificity; the vector is transformed into a receptor plant and human insulin-like growth factor-1 with biological activity is expressed in a transformed plant seed. The insulin-like growth factor-1 obtained by the method of the invention can be applied to treat IGF-1 insufficiency and deletion of growth hormone (GH) found in children or other clinical and lab researches.

The invention provides a piston cooling nozzle automatic measuring device comprising the following structures: a feeding system used for assembling a workpiece body with a nozzle body and placing the assembled workpiece on a measuring position; an oil supply system used for providing oil body for the workpiece measuring process and specially processing the oil body so as to follow regulated flow and / or pressure measuring conditions; a workpiece positioning system used for positioning the workpiece to the measuring position; a workpiece detection system used for measuring the workpiece related performance parameters; an unload system used for removing the workpiece from the measuring position and specially processing the workpiece; an intelligent control system used for controlling work states of all systems and transmitting data. The automatic measuring system employs a modularized structure, can accurately and precisely position the workpiece, thus fast respectively measuring the workpiece related work parameters, and realizing automatic measurement.

The invention provides a method for mining oil. The method comprises the steps that horizontal well groups are set up according to an oil storage layer; steam is injected into the horizontal well groups to enable the oil storage layer to form a steam cavity; a vertical well is set up according to the oil storage layer; and carbon dioxide is injected into the vertical well to enable an oil body inthe oil storage layer to be discharged by the horizontal well groups. According to the method for mining oil, the output of the oil can be effectively increased.

The invention relates to an anti-error refueling control platform and method. The platform comprises a refueling gun and type comparison equipment, wherein the refueling gun is arranged in a refuelingstation and corresponds to an independent refueling number, and the refueling number is used for identifying a position of the refueling gun and a refueling oil body type; the type comparison equipment is connected with the oil gun and is used for sending an oil body type alarm instruction when the refueling oil body type corresponding to the refueling number is inconsistent with a field oil bodytype; and the type comparison equipment is also used for sending an oil body type consistency instruction when the refueling oil body type corresponding to the refueling number is the same as the field oil body type. By using the anti-error refueling control platform and method, an application is wide and identification is effective. Because a condition that the oil body type of the refueling gunis not matched with the oil body type required by a vehicle can be found in time, a probability of refueling accidents is reduced.

The present invention provides novel emulsions that comprise oil bodies. The invention also relates to novel methods for generating formulations comprising oil bodies and active ingredients wherein the active ingredient is partitioned into the oil body. The methods are particularly useful for generating emulsions with either hydrophobic or amphipathic biologically active agents.