242 results about "Metallothionein" patented technology

Streptavidin-metallothionein chimeric proteins with biological recognition specificity in which the streptavidin moiety provides high affinity biotin binding and the metallothionein moiety provides a high affinity metal binding. The binding affinity of the streptavidin-metallothionein chimeric protein both for biotin and heavy metal ions allows specific incorporation into, conjugation with, or labelling of any biological material containing biotin with various heavy metal ions.

The invention relates to a fluorescent probe, wherein, the fluorescence is enhanced in the presence of ONOO-, and the fluorescence returns to original state in the presence of reducing matter. The invention provides a reversible fluorescent probe for selective detection of ONOO- in cells, characterized in that: cyanine dye is used as the fluorescent matrix, an organic selenide structure is introduced in the cyanine matrix as the active site of the reaction with ONOO- to realize the selective detection of ONOO-; the properties that organic selenium oxide formed by oxidizing organic selenide is easy to be reduced to organic selenide by reducing micromolecules in biosystem, such as cysteine, reducing glutathione, metallothionein and the like, is utilized to realize the reversibility of the probe molecules; and variations of electronic properties of organic selenide before and after the oxidation and the influence of the variations on the fluorescence property of the whole compound are utilized simultaneously to modulate the fluorescence property of the probe molecules.

Metallothionein is a stress response protein responsible for some forms of stress-related immunosuppression. An anti-metallothionein antibody can stimulate the humoral immune response to T-dependent antigens. An anti-metallothionein antibody is particularly useful when administered with an antigen to stimulate the immune response in a subject. An anti-metallothionein antibody can be administered to a subject with a vaccine to immunize the subject.

The invention discloses a halostachys caspica metallothionein gene as well as a recombinant protein and application thereof. The halostachys caspica metallothionein gene has a base sequence shown by SEQ ID NO.1 in a sequence table and has a full length of 234bp, and is used for coding a protein consisting of 78 amino acids in an amino acid sequence shown by SEQ ID NO.2 in the sequence table. The invention also discloses a recombinant expression vector and an expression transformant containing the gene, and a preparation method and application of halostachys caspica metallothionein. According to the invention, a new metallothionein gene is obtained through in vivo cloning of a Xinjiang saline alkali plant namely halostachys caspica; the recombinant metallothionein obtained through production of a genetic engineering expression transformant contains 14 cysteines without a disulfide bond, and is rich in sulfydryl groups which can be chelated with heavy metals including zinc, copper, cadmium and the like and can clean free radicals and resist ionizing radiation or ultraviolet irradiation, so that the recombinant metallothionein has a wide application field.

The invention discloses a production method of metallothioneins of cordyceps sinensis, particularly comprising the following steps of: taking cordyceps sinensis; carrying out liquid culture (adding zinc sulfate for induction); filtering mycelia to obtain mycelia which are rich in the metallothioneins; adding a buffer solution, and grinding to disrupt cells; adding an organic denatured solution, and stirring to denature foreign proteins; centrifugally removing the foreign proteins; heating a supernatant for denaturing; centrifugally removing denatured proteins; fast cooling to room temperature; carrying out ultrafiltration concentration to obtain a concentrated solution; carrying out chromatography through G-50; monitoring an MT (Metallothionein) eluent through a sulfhydryl reagent method,and collecting the MT eluent; and carrying out vacuum freeze drying on the MT eluent to obtain MT freeze-dried powder. The invention has the advantages of easiness in operation, short production period, high yield of MT contained in the mycelia or sporocarps and factory production.

The invention belongs to the technical field of production and preparation of health bedding fabrics for teenagers, and relates to a far infrared anti-electromagnetic wave body-perfuming sleeping bag used for teenagers, comprising nano selenium and traditional Chinese medicinal materials and capable of generating negative ions. Oxygen is necessary for life, life activities can not continue without oxygen, and a brain will die if no oxygen gas is supplied for 5 minutes, while 20% of oxygen forms oxygen radicals which are harmful for a human body during metabolism. The metallothionein in nano selenium-containing bee products has the effect of strongly scavenging free radicals, the nano powder of medical stone or tourmaline has a far infrared anti-electromagnetic wave function, and the molecules of nano silver antimicrobial agent and traditional Chinese medicinal materials such as sweet osmanthus flower, jasmine flower and peach flower penetrate into deep skin tissues at a depth of 3-5 mm. The sleeping bag provided by the invention can be automatically heated, and has the effects of promoting metabolism, tissue vitality and blood circulation and perfuming skin. The spontaneously generated negative ions have the effects of regulating surrounding air quality, enhancing immunity, and strongly killing bacteria and mites.

The invention belongs to the soil environment management field in the environmental engineering and relates to the construction and application of a new genetically engineered microorganism capable of restoring the soil polluted by heavy metal cadmium. The preparation method of the genetically engineered microorganism comprises the following steps: monkey metallothionein alpha structure domain tetramer is fused in the ice-nucleating protein N-end of Xanthomona campestris ATCC33913 to be used as a main functional unit for constructing a broad host range expression plasmid; then cadmium-specific promoter is used for expression in Pseudomonas putida X4 which can resist various heavy metals efficiently, thus realizing the surface display of monkey metallothionein alpha structure domain tetramer in the bacterium and obtaining genetically engineered microorganism CH01 which can adsorb heavy metal cadmium efficiently and specifically. The engineered microorganism can remarkably adsorb heavy metal cadmium in the presence of various heavy metals; and the result of the symbiotic water culture with wheat, cowpea and corn shows that the engineered microorganism CH01 can colonize in the rhizosphere of plant and fast adsorb cadmium ions. The invention also discloses a construction method of the genetically engineered microorganism CH01 of which preservation number is CGMCC No.M209320.

The invention relates to a liposome sun-screening skin care product, belonging to the technical field of cosmetics. The liposome sun-screening skin care product comprises the following components in parts by weight: 0.5-8.0 parts of epidermal growth factor (EGF), 0.01-0.1 part of human stem cell growth factor (SCGF), 10.0-10,000.0 parts of hyaluronic acid, 0.003-3000.0 parts of phospholipid, 0.001-10.0 parts of metallothionein, 0.001-10.0 parts of collagen, 10.0-5000.0 parts of water-soluble pearl powder the weight percentage of which is 20%, and 0.001-200.0 parts of cholesterol. The sun-screening skin care product provided by the invention has the beneficial effects that the liposome is composed of phospholipid bilayers and contains pure water micro vesicles, and the vesicles can enclose a certain amount of nutrients and essences, are 100 nanometers in mean diameter which is 1/200-1/300 of the human cell diameter, can easily penetrate through the surface layer of human skin and can be fused with cells, thus exerting efficacy. The liposome sun-screening skin care product can deeply humidify horny cells and enhance hydration to improve the skin softness, and can provide sufficient nutrients to ensure skin health and increase skin resistance.

The invention discloses a composition capable of protecting liver. The composition comprises fullerene evenly dispersed in edible oil, and further comprises at least one of vitamin C, vitamin E, catechinic acid, alpha-lipoic acid, anthocyanin, curcumin, carotenoid, plant polyphenolses, plant polysaccharides, animal and plant terpene, plant flavonoid, SOD (Superoxide Dismutase), coenzyme Q10, glutathione, lactoferrin and metallothionein evenly dispersed in the edible oil. The composition effectively protects the liver and reduces the damage of the negative factor on the mouse liver, so that the blood index of the liver function tends to be at the normal range, the excellent effect of scavenging a free radical is achieved, the liver is cleared, and the redox state of the liver is improved.

Metallothionein is extracted from pork liver by. taking porket as supply source, Intraperitoneal injecting with trace element zinc and inducing, Butchering after 5 times inducing, separating and extracting metallothionein which is well stability and high purity, with high output i.e. 2 to 3 times of extracted from rabbits. The pork liver metallothionein induced with zinc has advantages of low cost, good effect, high output, wide source of inducer, without side effect etc. And pig is a easy feeding animal, wide source, and the pork is edible after inducing to extract metallothionein, The products thereof adapted for anti-oxidation and anti irritability for avian and livestock's feedstuff.

The invention is concerned with the skin care compound medicine for scar restoration. It contents: metallothionein 50-200ppm, epidermal growth factor 5-60 thousands international unit/30ml, one or more types of additional skin care liveness materials and carriers are acceptable by skin. The invention boots up the speed of skin restoring, reduces scar and mark of burns, scald.

The present invention provides compositions and methods for regulating expression of heterologous nucleotide sequences in a plant. Compositions include a novel nucleotide sequence for a promoter for the gene encoding metallothionein. A method for expressing a heterologous nucleotide sequence in a plant using the promoter sequences disclosed herein is provided. The method comprises transforming a plant or plant cell with a nucleotide sequence operably linked to one of the promoters of the present invention.

The invention discloses an anti-stress fodder adjusting control agent for milk cows and a fodder containing the adjusting control agent. The fodder adjusting control agent is prepared from a pluralityof Chinese herbal raw materials, metallothionein course extract powder and alfalfa powder according to a certain proportion; and the fodder is prepared from the adjusting control agent and conventional fodder raw materials according to a certain proportion. A preparation method comprises the following steps: firstly, crushing various raw materials; secondly, adding the crushed components into solutions in a certain amount, dissolving the components, filtering the solutions, concentrating the filtrates and drying the concentrated filtrates, and obtaining extracts by separation; and finally, mixing the extracts, adding corn flour, soy bean meal and grass meal in certain proportion into the mixed extract, evenly stirring the mixture, and packing the mixture into a sealed bag for later use. The anti-stress adjusting control fodder for the milk cows has the advantages of reasonable formulation, wide raw material sources, low cost, feasible method and simple and convenient operation, avoidsadding any antibiotics or chemical medicine, has no residue or pollution, can effectively relieve the stress of the milk cows, improve the physiological function of the milk cows, particularly lactating cows, enhance the constitutions of the milk cows and improve the lactating amount of the milk cows and the quality of milk, and is particularly suitable for large-scale milk production.

The invention provides a preparation method of soluble recombinant sinopotamon metallothionein (rcMT), and the preparation method has the advantages of simplicity and convenience in operation, low cost, high purity and good activity. The method comprises the following steps: firstly constructing a phoA-MT recombinant expression vector by taking a phoA plasmid as an expression vector; further transforming escherichia coli BL21, constructing an escherichia coli engineering strain phoA-MT/BL21, and inoculating the engineering strain into an LB (Luria-Bertani) culture medium to prepare seeds, then diluting bacteria to a low-phosphorus culture medium for induction and expression, and utilizing Ni sepharoseTM6 Fast Flow affinity chromatography for purification so as to get the recombinant sinopotamon metallothionein. An anti-sinopotamon metallothionein monoclonal antibody prepared by utilizing the recombinant sinopotamon metallothionein has high sensitivity and strong specificity and can be applied to ELISA (enzyme-linked immunosorbent assay) quantitative assay of the metallothionein.

The invention discloses an anti-acne composition containing epidermal growth factors and tea tree oil and a preparation method thereof. The anti-acne composition disclosed by the invention comprises the following components: epidermal growth factors, tea tree oil, metallothionein, chlorhexidine, humectant, tackifier, alpha-carboxylic acid, pH value regulator, surfactant and deionized water. The preparation method of the anti-acne composition disclosed by the invention comprises the following steps of: mixing and heating the epidermal growth factors, the tea tree oil, the metallothionein, the chlorhexidine and the surfactant to be used as an oil phase; dissolving and heating the humectant, the tackifier and the alpha-carboxylic acid in water to be used as a water phase; adding the oil phase into the water phase, and stirring uniformly; and adding the pH value regulator for regulating the pH value, thereby obtaining the anti-acne composition containing epidermal growth factors and tea tree oil. The epidermal growth factors contained in the anti-acne composition disclosed by the invention can accelerate the metabolism of skin cells, and the natural tea tree oil capable of resisting bacteria and diminishing inflammation can efficiently sterilize bacteria, diminish inflammation and remove acnes, thus the anti-acne composition has good moisture retention and small skin irritation.

This invention provides a method for expressing human metallothionein (MT) by using plant oil. The method comprises: designing and synthesizing human MT gene according to the preference of plant codon, fusing human MT gene with plant oil protein gene, constructing an expression vector with the fused genes, converting recipient plant by using the expression vector to obtain converted plant, and efficiently expressing human MT by using the seed of the converted plant. Activity experiments show that purified recombinant MT has comparable activity with human origin MT. The method utilizes plant bioreactor to produce MT, thus can avoid possible attack by animal pathogen and toxin in E. coli. The method can largely simplify the separation and purification process of human MT, and lower the production cost, this is advantageous for MT industrialization.

The invention discloses a cream for anti-oxidation and improvement of the human immunity and a preparation method thereof, the invention is the cream which is prepared by taking metallothionein, coenzyme-Q10 chelate lipoic acid, sclareol, chamomile, L-carnitine, rosemary oil, cimicifuga racemosa, dextran, Kamm, jojoba oil, distilled water and collagen protein as raw materials, the cream has the advantages that the effects can be improved by a plurality of times of the effects before the chelation by the organic compound formula of a plurality of anti-oxidation natural organisms for improving the human immunity, such as, MT, coenzyme-Q10 chelate and so on, thus having good improving effects on swollen welling-abscess, rhinitis and asthma which are caused by the damages of the immune system and having great eliminating effect on the prevention of the tumors; furthermore, the skin can be moisturized, whitened and replenished with water by smearing the cream of the invention.

The invention discloses a magnetic biological material capable of adsorbing uranium (VI) in an aqueous solution and a preparation method of the magnetic biological material. The magnetic biological material has the particular name of gene recombinant saccharomyces cerevisiae microsphere, which is obtained by mixing human liver metallothionein gene recombinant saccharomyces cerevisiae with a ferromagnetic fluid and immobilizing by sodium alginate and calcium chloride; wherein the mixing ratio is that the mass ratio of gene recombinant saccharomyces cerevisiae bacteria to the ferromagnetic fluid is 10:1. The magnetic gene recombinant saccharomyces cerevisiae microsphere has such characteristics of gene recombinant saccharomyces cerevisiae as high uranium (VI) adsorption capacity, low adsorption cost, recyclable biomass and no secondary pollution, and is relatively convenient and rapid to recycle, thereby greatly enhancing the recycling ratio, reducing the uranium pollution degree, and meanwhile promoting the uranium resource recycling actively.

The invention relates to a method for inducing and extracting metallothionein from housefly larva, which comprises the following steps of: culturing 3-5-day housefly larva in a nonmetal culture dish by using a culture medium of a metal ion biological inductive agent of Cd2+, Zn2+ and Cu2+; and carrying out rinsing, sterilizing, low temperature and high speed homogenate, membrane filtration, chromatographic fractionation, elution and concentration, and vacuum freezing and drying on the housefly larva to obtain Zn/Cu metallothionein which has molecular weight of 5300-6500 dDa and contains 20-27 percent of cysteine and subtypes thereof, wherein the molecular structure of the Zn/Cu metallothionein takes less aromatic amino acids. The yield of the metallothionein in biologically induced larva protein can reach 1mg/g. The production method has low cost, high purity and high yield of metallothionein.

The invention relates to the field of controlled release of biomedical materials and drugs, and particularly relates to a nanoparticle preparation, and a preparation method and application thereof. The nanoparticle preparation is prepared from the following components: (1) oligopeptides and/or oligopeptides derivatives; and (2) metal ions. The nanoparticle preparation is uniform and controllable in particle size, high in drug loading ratio, and good in stability in blood, can quickly release drugs via multiple stimulated response of pH/enzyme/glutathione/metallothionein, and is easy in preparation method and easy for large-scale production.

The invention provides a repairing agent for heavy-metal-polluted soil and a resource recycling method of construction waste. The soil repairing agent comprises 40-60% of a construction waste treatment material, 10-20% of silica sol, 5-10% of humic acid and 10-45% of metallothionein; the construction waste treatment material is prepared through the following steps that the construction waste is broken into powder and then screened by a 1200-mesh molecular screen, a citric acid water solution is added and then stirring is conducted for one hour or more, then calcium polysulfide is added and then stirring is conducted for four hours or more, after a mixed solution is screened by the 1200-mesh molecular screen, still standing is conducted to subside, water is pumped out, sediments are dried, and after grinding, the construction waste treatment material is prepared. According to the repairing agent of the heavy-metal-polluted soil and the resource recycling method of the construction waste, resource recycling of the construction waste is achieved for the first time, and on the one hand, environment pollution can be effectively reduced, and waste treatment cost can be effectively lowered; and on the other hand, preparing cost of the prepared construction waste treatment material is remarkably lowered, the repairing property cannot be lowered, or the repairing time cannot be prolonged.