67 results about "Phosphatidylserine" patented technology

The invention provides a composition beneficial to improvement of brain cognition, memory and sleep and prevention and improvement of Alzheimer's disease, and belongs to the technical field of health food. The composition comprises L-alpha-glycerophosphorylcholine (GPC), phosphatidylserine (PS), docosahexaenoic acid (DHA), tea leaf theanine (L-theanine), vitamin B groups, vitamin D3 and the like, and is prepared into a health care product, a dietary supplement, a functional food, a medical food or a nutritional supplement by adopting the existing technology. The traditional Chinese medicine composition has the effects of effectively nourishing nerve cells, improving the brain sensitivity and improving the cognitive function. The composition is particularly directed at age-related cognitive decline and neurodegenerative diseases. Repair and regeneration of nerve cells can be promoted, the transmission efficiency of neural signals is enhanced, and the overall working efficiency of the brain is improved. Daily life challenges caused by cognitive decline can be relieved, and individual life quality can be improved.

The invention relates to a stem cell bioactive nano-vesicle with efficient inflammation regulation and tissue repair effects, the average particle size of the nano-vesicle is 100-350nm, the polydispersity index (PDI) is 0.05-0.40, and the expression rate of phosphatidylserine (PS) on the surface of the nano-vesicle is greater than 40%. According to the preparation method, stem cells are subjected to apoptosis induction, and then a programmed serial extrusion process is adopted to prepare the nano-vesicles. The nano vesicles are moderate in particle size, uniform in distribution and high in production efficiency, the preparation process is easy to operate, the quality is stable and controllable, and the nano vesicles have excellent technical effects in the aspects of repair and regeneration of inflammation injury type tissues and organs.

The present invention discloses a functional solid beverage for resisting altitude sickness and a preparation method thereof. The raw materials of the solid beverage include yak spleen small molecule polypeptide, yak milk small molecule polypeptide, taurine, phosphatidylserine, crystalline fructose and flaxseed; wherein the amino acid sequence of the yak spleen small molecule polypeptide is shown as SEQ ID NO.1, and the amino acid sequence of the yak milk small molecule polypeptide is shown as SEQ ID NO.2. The functional solid beverage of the present invention promotes oxygen carrying and improves the oxygen transport efficiency of red blood cells by reducing the deformation and adhesion of red blood cells caused by plateau exposure, thereby effectively preventing and alleviating altitude sickness such as headache, nausea, fatigue, etc. The product is portable and easy to use, the ingredients are safe and harmless, and the effect has been verified to be significant through experiments. The functional solid beverage of the present invention provides scientific and effective health protection for people entering high-altitude areas, and has broad market prospects and application value.

The invention relates to the technical field of cock breeding, in particular to a biomarker, a preparation method and application thereof and a method for assisting in breeding cock semen quality identification. The biomarker comprises the following components: phosphatidylcholine, lysophosphatidylcholine, phosphatidyl ethanolamine, lysophosphatidyl ethanolamine, phosphatidylserine and lysophosphatidylserine. According to the invention, key markers can be rapidly detected from the semen so as to determine breeding suitable for liquid-state preservation of the semen and high-activity individuals. The artificial insemination interval time can be greatly prolonged, and long-time high-sperm active breeding cock individuals can be rapidly bred. The breeder cock breeding amount can be reduced, grains are saved, and breeding benefits are greatly improved.

The invention discloses a docetaxel-based liposome drug delivery system as well as a preparation method and application thereof, and belongs to the technical field of anti-tumor nano-drugs. The delivery system is composed of an active pharmaceutical ingredient docetaxel and a specific excipient, and comprises egg yolk lecithin, phosphatidylserine, taraxasterol, protamine oligopeptide, a necessary stabilizer and a necessary pH regulator. Through an optimized preparation process, the nano drug delivery system with good stability is successfully constructed. Experiments show that the liposome is high in encapsulation efficiency and has a good application prospect.

A reagent for measuring activated partial thromboplastin time, the reagent comprising phosphatidylcholine (PC), phosphatidylserine (PS), and phosphatidylethanolamine (PE), wherein the ratio (PS / PC) of the concentration of the PS to the concentration of the PC is 0.26-2.00, and the concentration of the PS is 14-60 µg / mL.

The invention discloses application of phosphatidylserine-hydrocortisone microvesicles in preparation of a medicine for treating microvascular regeneration after cerebral apoplexy, and relates to the technical field of biomedicine. According to the microbubble, the blood brain barrier can be accurately opened through the ultrasonic vacuolation technology, the medicine can be delivered in a targeted mode, nerve protection and function improvement are accurately evaluated through ultrasonic super-resolution micro blood flow imaging, and microvessel regeneration is accurately regulated and controlled based on H3K18 lactic acid modification.

The invention discloses a multifunctional special dietary food skin beautifying and shaping formula and application, and belongs to the technical field of beauty treatment. The multifunctional special dietary food at least comprises collagen peptide, collagen tripeptide, skipjack elastin peptide, carob bean extract, phosphatidylserine, casein hydrolytic peptide, nicotinamide, rice bran oil powder, haematococcus pluvialis, cranberry powder, oriental cherry flower powder, pig blood peptide powder, red date peptide powder and cattle spleen peptide. The health-care product can comprehensively supplement nutrition recovered after exercise, has the effects of beautifying, shaping and removing wrinkles, can effectively relieve pressure and regulate emotion, and also has the effects of improving female physiological health, strengthening the spleen and stomach and enhancing immunity.

The invention provides a composition with a sleep aiding effect. The composition is prepared from phosphatidylserine, gamma-aminobutyric acid, enzymolysis oat and enzymolysis radix puerariae powder. Every 100 g of the composition is prepared from 0.7 g to 2.8 g of phosphatidylserine, 0.3 g to 1.3 g of gamma-aminobutyric acid, 1.5 g to 6.3 g of enzymolysis radix puerariae powder and 89.6 g to 97.4 g of enzymolysis oat powder. The composition with the sleep aiding effect is safe in components, scientific in proportion, convenient to carry and free of obvious side effects, and has a good effect on improving sleep.

The invention discloses a dietary therapy formula for preventing senile dementia, improving memory, repairing a conducted nervous system and dredging damaged brain nerves, and belongs to the technical field of dietary therapy health care. According to the formula, functional nutritional ingredients, bioactive peptides, medicinal and edible extracts and compound probiotics are taken as cores, and the formula is prepared through scientific proportioning; the health-care food specifically comprises xylitol, isomaltooligosacharide, N-acetylneuraminic acid, phosphatidylserine, bovine brain peptide, walnut peptide, shark peptide, acer truncatum seed oil microcapsule powder, DHA algal oil microcapsule powder, spina date seeds, tea theanine, gamma-aminobutyric acid, Belgian imported inulin, magnesium stearate, and 16 optional specific probiotics and metabiotics. The formula follows a multi-dimensional synergistic principle of nutrition supply-nerve repair-function regulation-absorption synergy, the core component can penetrate through a blood brain barrier, supplement nerve repair raw materials, repair damaged nerve cells and myelin sheaths and regulate neurotransmitter balance, and the probiotics indirectly assist nerve maintenance through intestinal tract-nerve axis, so that the composition has the effects of promoting blood circulation and promoting blood circulation. The synergistic effects of preventing senile dementia, improving memory and repairing and dredging a nervous system are achieved; the raw materials are food-grade safe components, have no side effects, can be prepared into powder, tablet candies and other forms, are convenient to eat and suitable for long-term conditioning, and solve the problems of single component, insufficient repair pertinence and low absorption efficiency of existing nerve care products.

The invention belongs to the technical field of functional food, and particularly relates to a brain-strengthening and eyesight-improving composition as well as a preparation method, a product and application thereof. The composition is prepared from algal oil, linseed oil, arachidonic acid, marigold extract, zeaxanthin suspension oil, NA nervonic acid and PS phosphatidylserine according to a specific weight part ratio. Wherein the marigold extract is prepared by adopting freeze-grinding pretreatment, combining enzymolysis and an ultrasonic-microwave synergistic extraction technology, and performing nanofiltration membrane concentration and vacuum freeze-drying. According to the composition, through the synergistic effect of all the components and an innovative extraction process, the effects of efficiently strengthening the brain and improving eyesight are achieved, and compared with a traditional formula and an extraction method, the component activity retention and the effective component extraction rate are remarkably improved.

The invention discloses a traditional Chinese medicine and non-traditional Chinese medicine combination formula for treating sub-health, and relates to the technical field of combination of traditional Chinese medicines and nutritional supplements.The traditional Chinese medicine and non-traditional Chinese medicine combination formula comprises 200 mg of lipoic acid, 100 mg of coenzyme Q10, 20 mg of pyrroloquinoline quinone, 3 g of xylooligosaccharide, 5 g of glutamine, 400 mg of plant lactic acid bacteria, 5 billion CFC of bifidobacterium longum, 300 mg of South African drunken solanum, 100 mg of rhodiola rosea, 200 mg of semen vaccariae extract, 50 mg of centella asiatica extract and 100 mg of phosphatidylserine. Compared with the prior art, the invention has the following advantages: the drug effect is stable; the effect is quick; the functions are comprehensive; the safety is high; the dosage forms are various.

The invention discloses an oral composition containing a vitamin C nicotinamide eutectic crystal. The composition comprises the following components in parts by weight: 15-35 parts of vitamin C-nicotinamide eutectic, active polysaccharide, phosphatidylserine, a natural calming component, astaxanthin, prebiotic oligosaccharide, lactoferrin, vitamin D3, an amino acid derivative and the like. The vitamin C and the nicotinamide exist in a eutectic form, so that the stability of the vitamin C and the bioavailability of the nicotinamide are improved, and the vitamin C and the nicotinamide synergistically exert an anti-oxidation effect. Meanwhile, various nutritional ingredients in the composition have a synergistic effect, so that the composition has comprehensive health-care effects in the aspects of supplementing nutrition, regulating immunity, improving sleep and the like.

To provide a lipid nanoparticle (LNP) for use in the delivery of therapeutic nucleic acids to cells.SOLUTION: Provided is use of (L-Serine) phosphatidylserine (PS) anionic targeting lipid in a total amount of 2.5-10 mol% of the total lipid content of a nucleic acid lipid nanoparticle (LNP) composition in the LNP composition.SELECTED DRAWING: Figure 15

The invention relates to a composition for improving cognitive decline and application thereof, a mouse aging model is constructed through a D-galactose injection method, and then mice are randomly divided into a control group, a D-gal model group, a DHA group, and a DHA, folic acid and phosphatidylserine combined intervention group (n = 12). And testing. Test results show that the combined intervention of DHA, folic acid and phosphatidylserine has a certain improvement effect on the cognitive function of the brain of an aging model mouse, can improve the inflammatory response in hippocampus tissues and the expression conditions of neurotrophic factors and synaptic associated proteins, regulates the in-vivo antioxidant level, and has a good anti-aging effect. And the new object identification and spatial learning memory ability of the mouse is improved. Therefore, the invention provides a theoretical basis for improving cognition and realizing functional dairy product development and industrial production through combined intervention of DHA, folic acid and phosphatidylserine.

The invention discloses a phospholipid hydrophilic chromatographic separation and evaporative light scattering detection method suitable for liquid milk, milk powder and milk source raw materials. The method comprises the following steps: preparing a sample solution from a sample to be detected; extracting the sample solution by using a solution of chloroform and methanol to obtain subnatant as extract liquor; adding a saturated salt solution into the extract liquor for chromatographic purification, and taking separated lower clear liquid as purified liquid; preparing the purified liquid into a sample introduction liquid; and determining the phospholipid content in the sample injection solution by using a liquid chromatography-evaporative light scattering detector. In the detection method, a sample solution is extracted by using a solution of chloroform and methanol, and a saturated salt solution is added into an extraction solution for chromatographic purification, so that chromatographic peaks are not interfered, and various phospholipids in food can be accurately measured at the same time; the method can be used for simultaneously determining phosphatidylcholine, sphingomyelin, phosphatidyl ethanolamine, phosphatidylserine and phosphatidylinositol in food, and has the advantages of simplicity, rapidness, accuracy, low detection limit and high sensitivity.

The invention relates to the technical field of eye health care and nanoemulsion, in particular to a nanoemulsion product for repairing eye health at night and a preparation method of the nanoemulsion product. The nanoemulsion product is prepared from algal oil (containing DHA), gamma-aminobutyric acid, phosphatidylserine, anthocyanin, N-acetylneuraminic acid and other functional components in a scientific proportion; and efficient dispersion and stable slow release are realized through a nano-emulsion preparation technology. The preparation method comprises the following steps: premixing algal oil and phospholipid, dissolving functional components, preparing a chitosan solution, performing high-pressure homogenization treatment, regulating and controlling the particle size and the like. The particle size of the product is 100-200nm, and the product has good dispersity, stability and biocompatibility, can quickly act on eye tissues through sublingual absorption, improves sleep quality and relieves asthenopia. The invention solves the problems of uneven particle size, insufficient stability and the like in the prior art, is suitable for night pupil protection scenes, and provides a comprehensive solution for eye health.

The application relates to a preparation method of immobilized phospholipase D and a preparation method of phosphatidylserine thereof, and belongs to the technical field of biotechnology.The preparation method of the immobilized phospholipase D comprises the following steps: (1) mixing mesoporous molecular sieve SBA-15 and a sodium acetate buffer solution to obtain a mixed solution; (2) mixing the mixed solution with an enzyme solution, oscillating, centrifuging, taking the precipitate, cleaning and freeze-drying to obtain the immobilized phospholipase D.The application improves the catalytic performance and stability of PLD by fixing PLD through the immobilization technology, realizes the reusability of PLD, and provides technical support for the industrialized production of the immobilized enzyme of PS.The preparation of phosphatidylserine by the immobilized PLD is optimized, and the optimal process condition of the preparation of PS by the immobilized PLD is obtained.The application further discloses a potential molecular mechanism of PS in treating AD, and provides a theoretical basis for the further clinical application of PS.

Zwitterionic phosphatidylserine (ZPS) monomers, ZPS polymers and ZPS copolymers, methods for making the ZPS monomers, ZPS polymers, and ZPS copolymers, compositions and materials that include ZPS polymers and ZPS copolymers, and methods for using the ZPS monomers, ZPS polymers, and ZPS copolymers.

The present invention relates to a liposome consisting of an outer leaflet comprising phosphatidylserine and an inner leaflet comprising phosphatidylserine and phosphatidic acid, characterized in that the zeta potential of the liposome is-25 mV to-100 mV and the molar ratio between total phosphatidylserine and phosphatidic acid is 5: 1 to 35: 1, more preferably 16: 1 to 18: 1, even more preferably 17: 1. The invention also relates to the use of said liposomes and / or the composition comprising said liposomes in the treatment of bacterial infections, preferably in the treatment of infections caused by Mycobacterium, in particular by non-tuberculous Mycobacterium.

The present invention belongs to the field of nanobiotechnology and discloses a plant-derived apoptosis-mimicking extracellular vesicle, a preparation method thereof, and an application thereof. The preparation method comprises the following steps: extracting plant-derived extracellular vesicles from plant tissues, and fusing the plant-derived extracellular vesicles with a solution containing apoptosis signal phosphatidylserine (PS) to obtain the plant-derived apoptosis-mimicking extracellular vesicles. The plant-derived apoptosis-mimicking extracellular vesicles of the present invention are derived from natural plants and have good biocompatibility compared to apoptotic cell vesicles derived from mammalian cells, and are less likely to cause immune rejection reactions in the body; after modification with phosphatidylserine (PS), compared with natural plant-derived extracellular vesicles, the plant-derived apoptosis-mimicking extracellular vesicles of the present invention have significantly improved macrophage targeting, inhibition of macrophage inflammatory response, and improvement of RA.

Functionalized nanoparticles for inhibiting or preventing pathogen infections (e.g., viral or bacterial infections, such as coronavirus infections) are described. The nanoparticles comprise a biodegradable polymer core and a lipid coating layer that is functionalized with a pathogen-binding receptor (e.g., an angiotensin-converting enzyme 2 (ACE2) receptor protein) and / or a pathogen-binding antibody or an antigen-binding fragment thereof (e.g., a virus-binding antibody or an antigen-binding fragment thereof). The nanoparticles are further functionalized by a phagocyte-specific ligand, e.g., a phosphatidylserine-containing lipid included in the lipid coating layer, to promote clearance of nanoparticle-bound pathogen. Methods of using the nanoparticles to treat or prevent pathogen infections (e.g., coronavirus infections) are also described.

The present invention relates to an entecavir liposome, a preparation thereof and a preparation method thereof, belonging to the technical field of antiviral drug preparations. The entecavir liposome of the present invention comprises entecavir, phosphatidylcholine, cholesterol, phosphatidylserine, and calcium lignosulfonate. The entecavir liposome of the present invention is obtained through film preparation, hydration, and liposome preparation. The present invention optimizes the formulation of the entecavir liposome, improves the stability of the entecavir liposome, enables the entecavir liposome to maintain the encapsulated state of the drug during storage and transportation, reduces the leakage of the drug, and thus improves the stability of the preparation.

The invention discloses a novel oil-soluble micelle preparation. The component A comprises lecithin, soya bean lecithin, egg yolk lecithin, 1-palmitoyl-2-oleoyl lecithin, phosphatidylserine, phosphatidylinositol, phosphatidyl glycerol, cardiolipin, cephalin and dipalmitoyl phosphatidylcholine, and the component B comprises lecithin, soya bean lecithin, egg yolk lecithin, 1-palmitoyl-2-oleoyl lecithin, phosphatidylserine, phosphatidylinositol, phosphatidyl glycerol, phosphatidylcholine, cephalin and dipalmitoyl phosphatidylcholine; one or more of dipalmitoyl phosphatidyl ethanolamine and dipalmitoyl phosphatidyl ethanolamine; the component B comprises a component B1 and a component B2, and the component B1 comprises one or more of phytosterol, cholesterol, ergosterol, protopanoxadiol, protopanaxatriol, ganoderic acid, phytol, isophytol, retinol, andrographolide, paclitaxel, artemisinin and bisabolol; and the component B2 is prepared from one or more of 1, 2-hexanediol, ethylhexylglycerin, cyclohexyl glycerin, absolute ethyl alcohol and isosorbide dimethyl ether. The key point of the invention is to provide a low-cost formula and a low-cost preparation method for preparing the oil-soluble micelle preparation.

The present disclosure belongs to the technical field of drug preparation, in particular to a composition and a soft capsule for treating attention deficit hyperactivity disorder (ADHD) in children, and their preparation method and use. In the present disclosure, phosphatidylserine is used as the core active ingredients. On this basis, ginkgo leaves extract and red ginseng extract are selected as supplementary active ingredients.

The invention discloses a fusion protein and a fluorescent microplate reader method for high-flux cell apoptosis detection, apoptotic cells are marked by fusion protein of annexin A5 (AnxA5) and fluorescent protein (FPs), and the fluorescence intensity of the fusion protein of AnxA5 and FPs is detected by a multifunctional microplate reader. And judging the exposure condition of the phosphatidylserine PS on the surface of the apoptotic cell according to the fluorescence signal. The method disclosed by the invention has the characteristics of simplicity and easiness in operation and rapidness in detection, and the method for detecting the fluorescence intensity by the microplate reader is applied to the detection of the cell apoptosis, so that the high-throughput detection of the cell apoptosis is realized. Compared with a flow cytometer and a fluorescence microscope, the multifunctional microplate reader has the advantages of low price, easiness in operation, simplicity in maintenance and easiness in popularization. The AnxA5 apoptosis detection probe is wide in selection range, is not limited by excitation wavelength and emission wavelength of a fluorescent marker, can simultaneously detect a plurality of samples at one time, and is convenient and fast.

The invention relates to a nano responder for enhancing magnetoacoustic coupling stimulation and a preparation method and application thereof. The nano responder comprises a magnetic nano bubble, the magnetic nano bubble comprises a core and a shell, the core comprises perfluoropropane, the shell comprises lipid, magnetic nano particles are encapsulated in the shell, and the surface of the shell is connected with phosphatidylserine and an anti-Piezo1 antibody. The targeted microglial cell Piezo1 magnetic nano bubble prepared by the invention can be used as a TMAS magnetic actuator, and can amplify the effect of TMAS and position the TMAS to a specific cell of a required brain area, so as to improve the stimulation effect and accuracy of TMAS.

A Streptomyces strain and the use thereof in the synthesis of phosphatidylserine. A fermentation broth produced by means of fermentation of the Streptomyces strain uses soy lecithin and L-serine as raw materials, and can simultaneously convert phosphatidylcholine (PC), phosphatidylethanolamine (PE), phosphatidylinositol (PI), etc. in lecithin to efficiently produce phosphatidylserine (PS). The Streptomyces strain is named Streptomyces sp. LN2, and was deposited in the China General Microbiological Culture Collection Center on 01 April 2024 with a deposit number of CGMCC NO. 30230.

The invention relates to the cross technical field of functional food and neuronutriology, and particularly discloses a brain nutrition support composition and application thereof. The brain nutrition support composition is prepared from epigallocatechin gallate, phosphatidylserine and N-acetylneuraminic acid, and multi-dimensional nutrition support can be provided for prevention and symptom improvement of the Alzheimer's disease through synergistic compounding of the three components; meanwhile, the brain nutrition support composition also has excellent stability and can be stored for a long time at room temperature; besides, in the long-term taking process of the brain nutrition support composition, pathological damage to liver and kidney functions and other important tissues and organs of a human body is not easily caused, and the brain nutrition support composition has good biological safety. The brain nutrition support composition provided by the invention can be further used for preparing various functional foods and dietary supplements, and solves the technical problems of single action mechanism, narrow range of applicable crowds, poor stability and the like generally existing in the existing brain nutrition support products.

Disclosed herein is a composition for sustained release of an active that includes a plurality of liposomal beadlets, wherein each of the plurality of liposomal beadlets comprise:a liposomal core comprising phosphatidylcholine (PC), phosphatidylethanolamine (PE), and phosphatidylserine (PS) wherein the active is dispersed within the liposomal core; a second core surrounding the liposomal core comprising one or more polypeptide and a polysaccharide; and a polymer beadlet shell surrounding the second core. In certain embodiments, the polymer beadlet shell contains gum arabic; microcrystalline cellulose (MCC); hydroxypropyl methylcellulose (HPMC); glycerol; γ-cyclodextrin; ethyl cellulose; and one or more metal halide salt.