70 results about "Nervonic acid" patented technology

The invention relates to the technical field of health food, in particular to a nervonic acid nutrient composition for relieving sleep disorder and application of the nervonic acid nutrient composition for relieving sleep disorder. The active components comprise natural vegetable oil such as acer truncatum seed oil purified nervonic acid, linseed oil and the like and gamma-aminobutyric acid extracted by fermentation; the synergistic component is prepared from soybean enzymolysis phosphatidylserine, saccharomycetes fermentation coenzyme Q10 and plant source B vitamins; the slow-release carrier adopts pectin-Arabic gum composite gel and plant starch to form a natural cross-linked structure, and long-acting release for 8-12 hours is realized through physical cross-linking. Green technologies such as ultrasonic dispersion and low-temperature drying are adopted in the preparation process, and chemical synthesis steps are avoided; the composition can improve the difficulty in falling asleep and improve the sleep quality, the activity retention rate is greater than or equal to 85% after the composition is stored at 45 DEG C for 6 months, and the composition is suitable for preparing capsules, gel candies and other health-care foods and has no dependence after being taken for a long time.

The invention provides a cosmetic application of nervonic acid. The cosmetic application is selected from the group consisting of anti-saccharification, energy metabolism, DNA damage repair, soothing and / or repair efficacy. The invention also relates to application of nervonic acid in preparation of an external skin preparation with anti-saccharification, energy metabolism, DNA damage repair, soothing and / or repair effects.

The invention discloses a self-emulsifying nervonic acid eye drop, which is prepared from the following raw material components in percentage by mass: 1 to 10 weight percent of nervonic acid, 30 to 60 weight percent of oil phase, 20 to 40 weight percent of surfactant and 10 to 20 weight percent of co-emulsifier, the oil phase is at least one of sunflower seed oil, squalane, olive oil and castor oil. According to the invention, the oil phase rich in unsaturated fatty acid is adopted, so that the eye cream has good solubility to nervonic acid, is mild and low-sensitive, can soften the cuticle of eyes and reduce the permeation resistance of nervonic acid, and is suitable for sensitive periocular skin. The prepared self-emulsifying nervonic acid eye drops overcome the defect that nervonic acid is almost insoluble in water, and compared with water-based eye drops, the self-emulsifying nervonic acid eye drops have the advantage that the drug loading capacity and the stability are obviously improved.

This invention relates to the field of oil and fat synthesis technology, specifically to a method for preparing nervonic acid monoglyceride. In this invention, nervonic acid and glycerol are used as raw materials, and an esterification reaction is carried out under microwave assistance to obtain a first mixture containing nervonic acid monoglyceride, nervonic acid diglyceride, nervonic acid triglyceride, unreacted nervonic acid and glycerol, and a catalyst. Glycerol and the catalyst are removed by ethyl acetate extraction to obtain a second mixture. Subsequently, taking advantage of the temperature-sensitive nature of nervonic acid monoglyceride in edible oils, it is separated and purified to obtain nervonic acid monoglyceride. The esterification reaction under microwave assistance not only avoids the corrosion and contamination problems caused by traditional acid catalysts, but also significantly shortens the esterification reaction time, improves synthesis efficiency, and effectively inhibits the isomerization of cis-nervonic acid during the reaction, ensuring the stability of the product configuration.

The invention relates to nervonic acid-loaded near-infrared light response type injectable hydrogel as well as a preparation method and application thereof, and belongs to the technical field of biological engineering. According to the invention, P (NIPAM-co-NHMAM) and a modified natural polymer material are combined to prepare a temperature-responsive hydrogel capable of realizing reversible volume shrinkage / expansion at a specific temperature, near-infrared light physiotherapy and irradiation are combined to damage local skin, and the hydrogel is stimulated to realize reversible volume deformation by controlling tissue temperature, so that the temperature-responsive hydrogel can be used for preparing the hydrogel with the reversible volume shrinkage / expansion at the specific temperature. Therefore, accurate and wireless space-time regulation and control of nervonic acid release behaviors are realized. Therefore, the nervonic acid loaded near-infrared light response type injectable hydrogel disclosed by the invention can realize efficient carrying and light response controllable release of nervonic acid, promotes functional recovery after spinal cord injury, and has a relatively good application prospect and research significance.

The invention provides a preparation method of acer truncatum seed oil, belonging to the technical field of vegetable oil processing, the acer truncatum seed oil prepared by the method contains higher content of nervonic acid, which has important significance for wider application of nervonic acid in the fields of biology, medicine and the like. Besides, the acer truncatum seed oil prepared by the method has more excellent antioxidant, anti-inflammatory and anti-aging effects, and can be added into cosmetics as a functional component so as to develop skin care products with multiple effects.

This invention relates to the field of nervonic acid preparation technology, specifically to a high-efficiency separation device for nervonic acid from garlic fruit oil based on molecular distillation technology. The device includes a molecular distillation apparatus, internally comprising, from top to bottom, a driver, a film scraping system, and a condensation system. The film scraping system includes a rotating shroud driven by the driver, a centrifugal disc located at the bottom of the rotating shroud, a pressure rod located inside the rotating shroud, and a transmission rod rotating at the bottom of the pressure rod and slidably inserted into the centrifugal disc. The centrifugal disc of this invention uses centrifugal force generated by high-speed rotation to evenly distribute the material onto the evaporation wall, forming an initial ultrathin film. Simultaneously, the scraper rotates accordingly, performing secondary scraping and homogenization of the initial liquid film. Utilizing the synergistic structure of centrifugal material distribution and mechanical scraping, the problem of uneven material distribution in traditional methods is solved at its source, and dynamic renewal of the evaporation interface is achieved, thereby improving heat and mass transfer efficiency.

The application relates to the technical field of nervonic acid purification, and discloses a nervonic acid purification device and a purification method thereof, which comprise a fixed-shell type flow guide mechanism and a rotary type filtering mechanism, the inside of the rotary type filtering mechanism is provided with an outer filter cylinder capable of rotating and filtering liquid, an inner filter cylinder capable of rotating and located at the center of the outer filter cylinder, and a horn collecting shell capable of making raw materials flow between the circumferential inner wall of the outer filter cylinder and the circumferential outer wall of the inner filter cylinder. The nervonic acid purification device and the purification method thereof filter nervonic acid liquid by using the bidirectional rotation of the inner filter cylinder and the outer filter cylinder. The rotation of the inner filter cylinder can prevent solid raw materials from adhering to the surface of the filtering holes of the inner filter cylinder, so that the filtering efficiency of the equipment is ensured. The rotation of the outer filter cylinder can make the solid raw materials adhered to the circumferential inner wall of the outer filter cylinder produce a centrifugal phenomenon, so that the nervonic acid liquid adhered to the inside of the solid raw materials can be thrown out, and the filtering effect of the equipment is ensured.

1. Name of the designed product: packaging box (DHA nervonic acid drink). 2. Use of the designed product: for product packaging. 3. Design points of the designed product: combination of shape and pattern. 4. Picture or photo best indicating the design points: perspective view 1.

The invention relates to the technical field of functional food, health care products and medicines, in particular to a composition for neuroprotection and cognitive function improvement and a preparation method and application thereof. The invention provides a composition for neuroprotection and cognitive function improvement. The composition is prepared from calcium beta-hydroxy-beta-methylbutyrate, nervonic acid and menthol, by compounding the calcium beta-hydroxy-beta-methylbutyrate, nervonic acid and menthol, the compound has a synergistic effect and can be applied to preparation of food, health care products or medicines for protecting nerves, delaying nerve aging, improving learning and memory ability, repairing nerve injury and preventing or assisting in treating neurodegenerative diseases.

The invention discloses a pharmaceutical composition for preventing and / or treating Parkinson's disease and application of the pharmaceutical composition, and relates to the technical field of biological medicine. The composition comprises nervonic acid, salvianolic acid A and pharmaceutically acceptable auxiliary materials. Experiments prove that the composition can improve intestinal flora, obviously promote alleviation of symptoms of a Parkinson's disease model animal, promote regeneration of dopaminergic neuronal cells and neural stem cells, relieve neuroinflammation, promote repair of damaged nerves, improve dyskinesia of mice caused by Parkinson's disease and improve the Parkinson's disease resistance of the mice. Therefore, the polypeptide has potential application value in treatment of the human Parkinson's disease. According to the invention, the effect of the composition is confirmed from the perspective of improving the change of intestinal flora by utilizing the relationship of the brain-intestinal axis, and a basis is provided for intervening the treatment of Parkinson's disease through the intestinal flora.

The invention discloses a recombinant Yarrowia lipolytica engineering strain for producing nervonic acid and application of the recombinant Yarrowia lipolytica engineering strain, and belongs to the technical field of genetic engineering and biological engineering. Beta-keto acyl-CoA synthase AtKCS, CraKCS and LaKCS are expressed in the Yarrowia lipolytica, recombinant Yarrowia lipolytica capable of synthesizing nervonic acid is obtained, supply of C18: 1 delta9-CoA is further enhanced, accumulation of C24: 0-CoA is reduced, endoplasmic reticulum is expanded, key genes are driven by a strong promoter, supply of malonyl CoA and NADPH is increased, and the yield of nervonic acid is increased, the nervonic acid yield of the engineering strain NA10 reaches 451.80 mg / L, and a foundation is laid for subsequent biosynthesis of lipid derivatives such as C24: 1-ceramide and the like.

The present invention relates to a composition for fat grafting containing adipose tissue treated with nervonic acid. According to the present invention, during fat tissue graft surgery, the survival rate and engraftment rate of fat included in grafted adipose tissue may be increased, thereby improving efficiency of fat tissue grafting.

The application discloses a pharmaceutical composition for treating or improving Parkinson's disease and application thereof. The application combines triterpene saponins and nervonic acid in different proportions, and proves through experiments that the combination in the specific proportion can significantly improve multiple indexes of Parkinson's mice, and has the effect of synergistically improving Parkinson's disease or the progress of Parkinson's disease. Therefore, the pharmaceutical composition of the application can be used for more effectively treating or improving Parkinson's disease, and has a wide application prospect in the treatment field of Parkinson's disease.

This invention discloses a nervonic acid-rich *Xanthoceras sorbifolium* diester oil and its preparation method, belonging to the field of oleochemical technology. The invention first removes some long-chain monounsaturated free fatty acids from *Xanthoceras sorbifolium* oil through saponification and low-temperature settling. Then, porous organic polymer materials are used to further enrich nervonic acids with higher molecular weight and larger particle size. Glycerol is added to the free fatty acids, and esterification with lipase catalyzes the synthesis of diglycerides, thus obtaining a nervonic acid-rich *Xanthoceras sorbifolium* diester oil. The addition of a compound antioxidant effectively prevents oxidative rancidity of the diester oil. The *Xanthoceras sorbifolium* diester oil prepared by this invention has a rich nervonic acid content, a high diglyceride content, and excellent thermal stability, thus showing good application prospects and value in the field of oleochemicals.

The invention discloses an extraction method of Brassica rapa seed oil, and belongs to the technical field of compound extraction. According to the method, green solvent dimethyl ether (DME) is combined with a response surface method to optimize process parameters (pressure, temperature and time) for extracting the turnip seed oil, fatty acid composition of the obtained turnip seed oil is analyzed through gas chromatography, and saponified matter of the turnip seed oil is prepared and used for follow-up activity and functional evaluation. After optimization, under the conditions that the extraction pressure is 6 MPa, the extraction temperature is 47 DEG C and the extraction time is 83 min, the DME extraction rate (31.72%) of the method is higher than that (30.94%) of Soxhlet extraction of a traditional extraction method. More importantly, rare unsaturated fatty acids such as nervonic acid and the like are effectively retained by lower extraction temperature, and 21 fatty acids are identified by component analysis. The unique fatty acid composition guaranteed by an optimized process is directly associated with the remarkable biological activity of the fatty acid composition. Core advantages of the method in turnip seed oil extraction are proved, and scientific basis and technical support are provided for industrialization of turnip seed oil in the field of cosmetics.

The invention provides a method for extracting nervonic acid from xanthoceras sorbifolia bunge. The method comprises the following steps: S1, performing low-temperature squeezing pretreatment on xanthoceras sorbifolia bunge kernels to obtain low-impurity crude oil; s2, performing ultrasonic-assisted PEG-4000 / ammonium sulfate aqueous two-phase extraction to enrich nervonic acid; s3, filtering through a composite polyether sulfone membrane loaded with amino modified montmorillonite-graphene, and intercepting impurities such as erucic acid in a targeted manner; s4, carrying out mild saponification and acidification; and S5, carrying out low-temperature gradient crystallization to obtain high-purity nervonic acid. Compared with the prior art, molecular distillation, a toxic solvent and a composite membrane are not needed, high-targeting interception-high-flux synergy is achieved, an aqueous two-phase system can be recycled, the final nervonic acid purity is larger than or equal to 99.5%, the extraction rate is larger than or equal to 0.8%, the waste liquid amount is reduced by 70%, the total consumed time is shortened to 10-12 h, and the problems that in the prior art, purity is low, pollution is large, and cost is high are solved.

The invention relates to the technical field of nervonic acid purification, and discloses nervonic acid purification equipment and a purification method thereof.The nervonic acid purification equipment comprises a fixed shell type flow guide mechanism and a rotary filtering mechanism; an outer filter cartridge capable of rotating and filtering liquid, an inner filter cartridge capable of rotating and located at the center of the outer filter cartridge, and a horn collecting shell capable of enabling raw materials to flow between the circumferential inner wall of the outer filter cartridge and the circumferential outer wall of the inner filter cartridge are arranged in the shell. According to the nervonic acid purification equipment and the purification method thereof, nervonic acid liquid is filtered by utilizing the inner filter cartridge and the outer filter cartridge which rotate in two directions, and solid raw materials can be prevented from being attached to the surfaces of filter holes of the inner filter cartridge through rotation of the inner filter cartridge, so that the filter efficiency of the equipment is ensured; the rotation of the outer filter cartridge can enable the solid raw material attached to the inner wall of the circumference of the outer filter cartridge to generate a centrifugal phenomenon, so that nervonic acid liquid attached to the interior of the solid raw material can be thrown out, and the filtering effect of the equipment is ensured.

The invention relates to the field of genetic breeding and molecular biology of shiny-leaved yellowhorn, in particular to a shiny-leaved yellowhorn nervonic acid content close-linkage SNP molecular marker and application thereof. According to the invention, a specific molecular marker and an amplification primer combination thereof are further designed on the basis of an SNP site which is remarkably associated with nervonic acid content control on a Xanthoceras sorbifolia Bunge chromosome 3; therefore, the SNP site closely linked with the nervonic acid content of the xanthoceras sorbifolia bunge, the molecular marker of the SNP site, application of the SNP site to identification of a xanthoceras sorbifolia bunge variety with high nervonic acid content and the identification method are constructed, the identification method is high in accuracy, the identification efficiency is greatly improved, and a practical tool capable of being popularized is provided for large-scale germplasm resource screening. In addition, the SNP marker is located on the intron of the KCS gene, so that a theoretical basis is provided for subsequent research of the KCS gene; more importantly, convenience is provided for work such as variety character improvement, and development of the SNP marker has important significance on cultivation of the shiny-leaved yellowhorn variety with the high nervonic acid content.

Provided herein are compositions and methods for enhancing immune development and responses in newborns. In particular embodiments, pre-natal and post-natal supplements and or infant formula and or follow-on formula (FOF) are provided comprising long chain fatty acids (LCFAs) and very long chain fatty acids (VLCFAs), such as trans-vaccenic acid (TVA), cis-11,14-Eicosadienoic acid (EDA), and nervonic acid (NVA), or derivatives thereof.

The invention discloses a double-target nerve conduction supporting nutritional composition and a preparation method thereof, and belongs to the technical field of nutritional products. The composition is prepared by compounding DHA algal oil, phosphatidylserine, EGCG and B vitamins with xanthoceras sorbifolia bunge oil nervonic acid and magnesium L-threonate which serve as dual-core targets in parts by mass, a'myelin sheath repair + signal transduction acceleration 'dual-target synergistic system is formed, membrane structure maintenance, oxidation resistance, inflammation resistance and neurotransmitter synthesis support are achieved at the same time, and the composition has the advantages of being simple in preparation process, low in cost and easy to popularize and apply. Neural conduction efficiency is systematically improved and instruction-execution latency is shortened. The problems of non-uniform mixing and caking of liquid oil and powder are solved through microencapsulation, the process is mature, the safety is high, and the stability is good. Animal experiments show that the composition can shorten the time of simple reaction by 30.5% (plt, 0.01) and shorten the time of selective reaction by 29.9% (plt, 0.01), has a non-obvious and unexpected technical effect, and is suitable for long-term nutritional supplement of middle-aged and elderly people and stroke convalescent people.

This invention provides a genetically engineered *Yersinia lipolytica* strain and its applications. The genetically engineered strain is a *Yersinia lipolytica* Po1f strain with the PEX10 gene knocked out, expressing the C16 / 18 elongase gene MaELO3 from *Morchella alpina*, the elongase gene AtKCS from *Arabidopsis thaliana*, and the elongase gene CraKCS from *Brassica oleracea*. The strain also expresses the elongase gene CgKCS from *Capsella*. Further expression of the desaturase gene MaD15D from *Morchella alpina*, and overexpression of the strain's own diacylglycerol transferase gene DGA1 and its own Δ9 desaturase gene OLE1, resulted in a nervonic acid yield of 111.6 mg / L in shake-flask fermentation. By adding 1% rapeseed oil exogenously, the nervonic acid yield reached 185.1 mg / L. The *Yersinia lipolytica* genetically engineered strain constructed in this invention is simple to operate, has stable and reliable performance, and can be applied to large-scale commercial production.

The invention relates to a method for preparing nervonic acid. The method comprises the following steps: S1, enabling erucic acid to react with acrolein under light irradiation in the presence of a photosensitizer, so as to obtain a compound as shown in a formula (I); the photosensitizer is selected from CeCl3 and / or Fe (OAc) 2; and S2, carrying out oxidation reaction on the compound as shown in the formula (I) prepared in the step S1 to obtain the nervonic acid. The method provided by the invention has the advantages of simple reaction steps and low cost.

The invention provides a medicine for preventing and relieving Alzheimer's disease and a preparation method thereof. Comprising a capsule-shaped medicine main body, and three coatings are arranged in the capsule-shaped medicine main body and are respectively a first coating section, a second coating section and a third coating section from outside to inside; the pharmaceutical composition comprises antioxidants such as SOD, vitamin C, vitamin E and anthocyanin, nerve repairing components such as nervonic acid, ganglioside and vitamin B12 and neurotransmitter regulators such as arginine, tryptophan and asparaginic acid, can block multiple pathogenic links of the Alzheimer's disease at the same time, remarkably improves the curative effect, adopts a three-stage coating technology, and is free of toxic and side effects. According to the present invention, different components are ensured to be accurately released at the specific site and within the specific time, the mutual interference among the components is avoided, the drug activity is maximized, the ROS in the body is cleared through the SOD, the vitamin E and the like, the antioxidant activity is stabilized by the anthocyanin, the nerve cell repair is promoted by the nervonic acid and the ganglioside, and the brain function is significantly improved;

The invention relates to a preparation method and application of a nervonic acid polyclonal antibody. When the prepared nervonic acid polyclonal antibody is used for detecting nervonic acid of an animal body, an instrument analysis method which is high in equipment dependence and complex in operation is abandoned, a rapid detection system based on competitive ELISA is constructed, and accurate quantification of nervonic acid in a sample is achieved by optimizing antibody coating concentration, enzyme-labeled antigen dilution proportion, reaction time and closing conditions. A reaction system is systematically optimized, so that the detection limit is reduced to 3.113 mu g / mL, and the within-batch precision is CV% lt; 6%, inter-batch CV% lt; the cross reaction rate with six common fatty acids is 0%, the detection performance is remarkably improved, and the clinical diagnosis and scientific research requirements are met.

The present application relates to the genetic breeding field of Xanthoceras sorbifolia Bunge, in particular to a Xanthoceras sorbifolia Bunge nervonic acid content tightly linked SNP molecular marker and application thereof. The present application further designs specific molecular markers and primer combinations thereof based on the SNP site on the 3rd chromosome of Xanthoceras sorbifolia Bunge, which is significantly associated with the control of nervonic acid content, thereby constructing the SNP site tightly linked to the nervonic acid content of Xanthoceras sorbifolia Bunge, the molecular marker thereof, the application thereof in identifying Xanthoceras sorbifolia Bunge varieties with high nervonic acid content, and the identification method. The identification method has high accuracy and greatly improves the identification efficiency, and provides a practical tool that can be popularized for large-scale germplasm resource screening. In addition, the SNP marker of the present application is located on the intron of the KCS gene, which provides a theoretical basis for subsequent KCS gene research. The present application provides convenience for variety trait improvement and the like, and the development of the SNP marker has important significance for the cultivation of Xanthoceras sorbifolia Bunge varieties with high nervonic acid content.

The invention relates to the technical field of gene editing, in particular to sgRNA for targeted knockout of a Cers2 gene based on a cas12i3 knockout system and application of the sgRNA. The sgRNA can efficiently and accurately knock out the Cers2 gene at the genome level, and a reliable gene editing tool and an experimental model are provided for researching the effects of the Cers2 in reproductive character regulation and control, ovarian lipid homeostasis, immune homeostasis and nervonic acid metabolism.

The invention discloses a method for microwave-assisted extraction of nervonic acid in xanthoceras sorbifolia bunge by a deep eutectic solvent method, and belongs to the technical field of nervonic acid extraction. The method comprises the following steps: S1, crushing xanthoceras sorbifolia bunge seed kernels to obtain xanthoceras sorbifolia bunge seed kernel powder; s2, mixing and soaking the shiny-leaved yellowhorn seed kernel powder and an intelligent deep-eutectic solvent to obtain a mixed solution; s3, performing microwave three-section treatment on the mixed solution to obtain an extracting solution; s4, enabling the extracting solution to pass through an APTES-SiO2 column, crystallizing by adopting a pulsed electric field, centrifugally purifying, washing and drying to obtain nervonic acid. The extraction method is simple in step, high-temperature treatment and multi-step fractionation are not needed, the nervonic acid yield exceeds 80%, the nervonic acid purity exceeds 97%, the mixing amount of oleic acid is small, and phospholipid residues are small.

The present invention belongs to the technical field of biochemistry; and specifically relates to an anti-tumor prodrug, and a preparation method therefor and the use thereof. The anti-tumor prodrug is composed of the tail of nervonic acid and the head of a hydrophilic chemotherapeutic drug. The hydrophilic chemotherapeutic drug is gemcitabine, and the tumor is glioma. An acid-sensitive amphiphilic prodrug compound is synthesized on the basis of using a nutrient nervonic acid capable of penetrating the blood-brain barrier and a chemotherapeutic drug as raw materials. Once the prodrug compound reaches a tumor site, an acid-sensitive bond is automatically cleaved due to the low pH in the tumor microenvironment, causing the prodrug compound to separate into the nervonic acid and the chemotherapeutic drug to perform respective functions thereof, wherein the chemotherapeutic drug kills glioma cells, and the nervonic acid repairs nerve tissues, thereby fundamentally solving the toxic side effects of the chemotherapeutic drug, and integrating therapeutic and rehabilitative effects.