42 results about "Nervonic acid" patented technology

This invention relates to the field of oil and fat synthesis technology, specifically to a method for preparing nervonic acid monoglyceride. In this invention, nervonic acid and glycerol are used as raw materials, and an esterification reaction is carried out under microwave assistance to obtain a first mixture containing nervonic acid monoglyceride, nervonic acid diglyceride, nervonic acid triglyceride, unreacted nervonic acid and glycerol, and a catalyst. Glycerol and the catalyst are removed by ethyl acetate extraction to obtain a second mixture. Subsequently, taking advantage of the temperature-sensitive nature of nervonic acid monoglyceride in edible oils, it is separated and purified to obtain nervonic acid monoglyceride. The esterification reaction under microwave assistance not only avoids the corrosion and contamination problems caused by traditional acid catalysts, but also significantly shortens the esterification reaction time, improves synthesis efficiency, and effectively inhibits the isomerization of cis-nervonic acid during the reaction, ensuring the stability of the product configuration.

This invention relates to the field of nervonic acid preparation technology, specifically to a high-efficiency separation device for nervonic acid from garlic fruit oil based on molecular distillation technology. The device includes a molecular distillation apparatus, internally comprising, from top to bottom, a driver, a film scraping system, and a condensation system. The film scraping system includes a rotating shroud driven by the driver, a centrifugal disc located at the bottom of the rotating shroud, a pressure rod located inside the rotating shroud, and a transmission rod rotating at the bottom of the pressure rod and slidably inserted into the centrifugal disc. The centrifugal disc of this invention uses centrifugal force generated by high-speed rotation to evenly distribute the material onto the evaporation wall, forming an initial ultrathin film. Simultaneously, the scraper rotates accordingly, performing secondary scraping and homogenization of the initial liquid film. Utilizing the synergistic structure of centrifugal material distribution and mechanical scraping, the problem of uneven material distribution in traditional methods is solved at its source, and dynamic renewal of the evaporation interface is achieved, thereby improving heat and mass transfer efficiency.

The application relates to the technical field of nervonic acid purification, and discloses a nervonic acid purification device and a purification method thereof, which comprise a fixed-shell type flow guide mechanism and a rotary type filtering mechanism, the inside of the rotary type filtering mechanism is provided with an outer filter cylinder capable of rotating and filtering liquid, an inner filter cylinder capable of rotating and located at the center of the outer filter cylinder, and a horn collecting shell capable of making raw materials flow between the circumferential inner wall of the outer filter cylinder and the circumferential outer wall of the inner filter cylinder. The nervonic acid purification device and the purification method thereof filter nervonic acid liquid by using the bidirectional rotation of the inner filter cylinder and the outer filter cylinder. The rotation of the inner filter cylinder can prevent solid raw materials from adhering to the surface of the filtering holes of the inner filter cylinder, so that the filtering efficiency of the equipment is ensured. The rotation of the outer filter cylinder can make the solid raw materials adhered to the circumferential inner wall of the outer filter cylinder produce a centrifugal phenomenon, so that the nervonic acid liquid adhered to the inside of the solid raw materials can be thrown out, and the filtering effect of the equipment is ensured.

The invention relates to the technical field of functional food, health care products and medicines, in particular to a composition for neuroprotection and cognitive function improvement and a preparation method and application thereof. The invention provides a composition for neuroprotection and cognitive function improvement. The composition is prepared from calcium beta-hydroxy-beta-methylbutyrate, nervonic acid and menthol, by compounding the calcium beta-hydroxy-beta-methylbutyrate, nervonic acid and menthol, the compound has a synergistic effect and can be applied to preparation of food, health care products or medicines for protecting nerves, delaying nerve aging, improving learning and memory ability, repairing nerve injury and preventing or assisting in treating neurodegenerative diseases.

The invention discloses a recombinant Yarrowia lipolytica engineering strain for producing nervonic acid and application of the recombinant Yarrowia lipolytica engineering strain, and belongs to the technical field of genetic engineering and biological engineering. Beta-keto acyl-CoA synthase AtKCS, CraKCS and LaKCS are expressed in the Yarrowia lipolytica, recombinant Yarrowia lipolytica capable of synthesizing nervonic acid is obtained, supply of C18: 1 delta9-CoA is further enhanced, accumulation of C24: 0-CoA is reduced, endoplasmic reticulum is expanded, key genes are driven by a strong promoter, supply of malonyl CoA and NADPH is increased, and the yield of nervonic acid is increased, the nervonic acid yield of the engineering strain NA10 reaches 451.80 mg / L, and a foundation is laid for subsequent biosynthesis of lipid derivatives such as C24: 1-ceramide and the like.

The application discloses a pharmaceutical composition for treating or improving Parkinson's disease and application thereof. The application combines triterpene saponins and nervonic acid in different proportions, and proves through experiments that the combination in the specific proportion can significantly improve multiple indexes of Parkinson's mice, and has the effect of synergistically improving Parkinson's disease or the progress of Parkinson's disease. Therefore, the pharmaceutical composition of the application can be used for more effectively treating or improving Parkinson's disease, and has a wide application prospect in the treatment field of Parkinson's disease.

This invention discloses a nervonic acid-rich *Xanthoceras sorbifolium* diester oil and its preparation method, belonging to the field of oleochemical technology. The invention first removes some long-chain monounsaturated free fatty acids from *Xanthoceras sorbifolium* oil through saponification and low-temperature settling. Then, porous organic polymer materials are used to further enrich nervonic acids with higher molecular weight and larger particle size. Glycerol is added to the free fatty acids, and esterification with lipase catalyzes the synthesis of diglycerides, thus obtaining a nervonic acid-rich *Xanthoceras sorbifolium* diester oil. The addition of a compound antioxidant effectively prevents oxidative rancidity of the diester oil. The *Xanthoceras sorbifolium* diester oil prepared by this invention has a rich nervonic acid content, a high diglyceride content, and excellent thermal stability, thus showing good application prospects and value in the field of oleochemicals.

The invention discloses an extraction method of Brassica rapa seed oil, and belongs to the technical field of compound extraction. According to the method, green solvent dimethyl ether (DME) is combined with a response surface method to optimize process parameters (pressure, temperature and time) for extracting the turnip seed oil, fatty acid composition of the obtained turnip seed oil is analyzed through gas chromatography, and saponified matter of the turnip seed oil is prepared and used for follow-up activity and functional evaluation. After optimization, under the conditions that the extraction pressure is 6 MPa, the extraction temperature is 47 DEG C and the extraction time is 83 min, the DME extraction rate (31.72%) of the method is higher than that (30.94%) of Soxhlet extraction of a traditional extraction method. More importantly, rare unsaturated fatty acids such as nervonic acid and the like are effectively retained by lower extraction temperature, and 21 fatty acids are identified by component analysis. The unique fatty acid composition guaranteed by an optimized process is directly associated with the remarkable biological activity of the fatty acid composition. Core advantages of the method in turnip seed oil extraction are proved, and scientific basis and technical support are provided for industrialization of turnip seed oil in the field of cosmetics.

Provided herein are compositions and methods for enhancing immune development and responses in newborns. In particular embodiments, pre-natal and post-natal supplements and or infant formula and or follow-on formula (FOF) are provided comprising long chain fatty acids (LCFAs) and very long chain fatty acids (VLCFAs), such as trans-vaccenic acid (TVA), cis-11,14-Eicosadienoic acid (EDA), and nervonic acid (NVA), or derivatives thereof.

The invention discloses a double-target nerve conduction supporting nutritional composition and a preparation method thereof, and belongs to the technical field of nutritional products. The composition is prepared by compounding DHA algal oil, phosphatidylserine, EGCG and B vitamins with xanthoceras sorbifolia bunge oil nervonic acid and magnesium L-threonate which serve as dual-core targets in parts by mass, a'myelin sheath repair + signal transduction acceleration 'dual-target synergistic system is formed, membrane structure maintenance, oxidation resistance, inflammation resistance and neurotransmitter synthesis support are achieved at the same time, and the composition has the advantages of being simple in preparation process, low in cost and easy to popularize and apply. Neural conduction efficiency is systematically improved and instruction-execution latency is shortened. The problems of non-uniform mixing and caking of liquid oil and powder are solved through microencapsulation, the process is mature, the safety is high, and the stability is good. Animal experiments show that the composition can shorten the time of simple reaction by 30.5% (plt, 0.01) and shorten the time of selective reaction by 29.9% (plt, 0.01), has a non-obvious and unexpected technical effect, and is suitable for long-term nutritional supplement of middle-aged and elderly people and stroke convalescent people.

This invention provides a genetically engineered *Yersinia lipolytica* strain and its applications. The genetically engineered strain is a *Yersinia lipolytica* Po1f strain with the PEX10 gene knocked out, expressing the C16 / 18 elongase gene MaELO3 from *Morchella alpina*, the elongase gene AtKCS from *Arabidopsis thaliana*, and the elongase gene CraKCS from *Brassica oleracea*. The strain also expresses the elongase gene CgKCS from *Capsella*. Further expression of the desaturase gene MaD15D from *Morchella alpina*, and overexpression of the strain's own diacylglycerol transferase gene DGA1 and its own Δ9 desaturase gene OLE1, resulted in a nervonic acid yield of 111.6 mg / L in shake-flask fermentation. By adding 1% rapeseed oil exogenously, the nervonic acid yield reached 185.1 mg / L. The *Yersinia lipolytica* genetically engineered strain constructed in this invention is simple to operate, has stable and reliable performance, and can be applied to large-scale commercial production.

The invention relates to a method for preparing nervonic acid. The method comprises the following steps: S1, enabling erucic acid to react with acrolein under light irradiation in the presence of a photosensitizer, so as to obtain a compound as shown in a formula (I); the photosensitizer is selected from CeCl3 and / or Fe (OAc) 2; and S2, carrying out oxidation reaction on the compound as shown in the formula (I) prepared in the step S1 to obtain the nervonic acid. The method provided by the invention has the advantages of simple reaction steps and low cost.

The invention relates to a preparation method and application of a nervonic acid polyclonal antibody. When the prepared nervonic acid polyclonal antibody is used for detecting nervonic acid of an animal body, an instrument analysis method which is high in equipment dependence and complex in operation is abandoned, a rapid detection system based on competitive ELISA is constructed, and accurate quantification of nervonic acid in a sample is achieved by optimizing antibody coating concentration, enzyme-labeled antigen dilution proportion, reaction time and closing conditions. A reaction system is systematically optimized, so that the detection limit is reduced to 3.113 mu g / mL, and the within-batch precision is CV% lt; 6%, inter-batch CV% lt; the cross reaction rate with six common fatty acids is 0%, the detection performance is remarkably improved, and the clinical diagnosis and scientific research requirements are met.

The present application relates to the genetic breeding field of Xanthoceras sorbifolia Bunge, in particular to a Xanthoceras sorbifolia Bunge nervonic acid content tightly linked SNP molecular marker and application thereof. The present application further designs specific molecular markers and primer combinations thereof based on the SNP site on the 3rd chromosome of Xanthoceras sorbifolia Bunge, which is significantly associated with the control of nervonic acid content, thereby constructing the SNP site tightly linked to the nervonic acid content of Xanthoceras sorbifolia Bunge, the molecular marker thereof, the application thereof in identifying Xanthoceras sorbifolia Bunge varieties with high nervonic acid content, and the identification method. The identification method has high accuracy and greatly improves the identification efficiency, and provides a practical tool that can be popularized for large-scale germplasm resource screening. In addition, the SNP marker of the present application is located on the intron of the KCS gene, which provides a theoretical basis for subsequent KCS gene research. The present application provides convenience for variety trait improvement and the like, and the development of the SNP marker has important significance for the cultivation of Xanthoceras sorbifolia Bunge varieties with high nervonic acid content.

The present invention belongs to the technical field of biochemistry; and specifically relates to an anti-tumor prodrug, and a preparation method therefor and the use thereof. The anti-tumor prodrug is composed of the tail of nervonic acid and the head of a hydrophilic chemotherapeutic drug. The hydrophilic chemotherapeutic drug is gemcitabine, and the tumor is glioma. An acid-sensitive amphiphilic prodrug compound is synthesized on the basis of using a nutrient nervonic acid capable of penetrating the blood-brain barrier and a chemotherapeutic drug as raw materials. Once the prodrug compound reaches a tumor site, an acid-sensitive bond is automatically cleaved due to the low pH in the tumor microenvironment, causing the prodrug compound to separate into the nervonic acid and the chemotherapeutic drug to perform respective functions thereof, wherein the chemotherapeutic drug kills glioma cells, and the nervonic acid repairs nerve tissues, thereby fundamentally solving the toxic side effects of the chemotherapeutic drug, and integrating therapeutic and rehabilitative effects.

This invention provides a composite composition containing nervonic acid and its use in brain nerve repair, belonging to the field of biotechnology. The invention uses nervonic acid and phosphatidylserine, combined with plant extracts fermented by a microbial concoction of *Lactobacillus acidophilus*: *Lactobacillus rhamnosus*: *Bacillus belyssae* = (2-3):1:1, to form a composite composition. This scientific formulation achieves synergistic effects from multiple components, improving neurological deficits, reducing cerebral infarction volume, decreasing the release of neurotrophic markers, and upregulating neurotrophic factor expression through multi-target synergistic effects. Furthermore, this synergistic effect depends on specific component ratios, providing crucial experimental support for the development of subsequent brain-protective functional compositions. This composition can be widely applied to the preparation of brain nerve repair drugs, functional foods, and dietary supplements, possessing promising industrialization prospects and clinical value.

The invention relates to the technical field of solid beverages, and particularly discloses an acer truncatum seed oil composite solid beverage rich in nervonic acid and a preparation method of the acer truncatum seed oil composite solid beverage. The invention discloses an acer truncatum seed oil composite solid beverage. The acer truncatum seed oil composite solid beverage comprises the following components: 15-20 parts of acer truncatum seed oil, 40-50 parts of a carrier, 0.3-0.5 part of glycerin monostearate, 5-10 parts of a plant extract, 1-2 parts of erythritol, 0.01-0.03 part of sucralose and 0.1-0.3 part of an anti-caking agent, the carrier is formed by compounding maltodextrin, soybean protein powder and beta-cyclodextrin according to the weight ratio of 20: (0.5-3): (3-7); the plant extract is prepared by compounding a white kidney bean water extract and a fructus forsythiae alcohol extract in a weight ratio of (10-17): (1-5). According to the technical scheme provided by the invention, the prepared acer truncatum seed oil composite solid beverage has relatively strong anti-sugar and anti-oxidation effects, and the stability of the solid beverage is excellent.

In one aspect, the disclosure relates to lubricant compositions comprising an oil, a wax, or a combination thereof, wherein at least one of the oil or the wax comprises a very long chain fatty acid; and wherein at least one of the oil or the wax has been modified to increase a concentration of the very long chain fatty acid relative to a reference concentration of the very long chain fatty acid in the oil or the wax without the modification. In a further aspect, the VLC FA can include one or more of eicosenoic acid, erucic acid, or nervonic acid.

The use of a nutritional composition is disclosed. The nutritional composition comprises medium- and long-chain fatty acid triglycerides and nervonic acid in a mass ratio of 1:1 to 5000:1. This nutritional composition has a synergistic effect in improving immunity, particularly in restoring the number of one or more macrophages, neutrophils, and T cells in immunocompromised individuals.

The application relates to the technical field of external preparation, and discloses a Xing-guan-guo nervonic acid external anti-psoriasis bacteriostatic cream and a preparation process thereof. The cream is prepared from Xing-guan-guo nervonic acid, behenyl alcohol, isostearic acid, cetyl stearyl glucoside, octyldodecanol, dicaprylyl carbonate and purified water. In the formula, the steric hindrance of octyldodecanol is used in cooperation with the polar dispersing force of dicaprylyl carbonate to keep the nervonic acid molecules in a free state and inhibit the crystallization and precipitation of the nervonic acid molecules in the cooling process. The behenyl alcohol and the isostearic acid in the system self-assemble to form a biomimetic lamellar liquid crystal framework at the oil-water interface under the guidance of the cetyl stearyl glucoside. The application solves the technical problem of high cohesion and easy aggregation of the nervonic acid, the constructed liquid crystal phase structure is compatible with the skin keratin layer lipid, the transdermal diffusion resistance of the effective component is reduced, and the long-term physical stability and the anti-psoriasis efficacy of the cream are improved.

The present invention relates generally to the technical field of nutrition products, and more particularly, to a nervonic acid composition for improving memory-based learning function and a preparation method thereof. The present invention develops a multi-component and multi-efficacy nervonic acid composition, and the synergistic interaction of various nutrients contained in the composition gives the nervonic acid composition of the invention excellent effect of improving memory-based. learning function. The lysolecithin and resistant dextrin added in the preparation process of the nervonic acid composition can stabilize a variety of poorly stable and easily oxidized functional ingredients contained in the composition, including nervonic acid, DHA and arachidonic acid. The resulting nervonic acid composition has a longer shelf life and improved bioavailability of the functional ingredients in the composition.

The application belongs to the technical field of pharmaceutical crystal, and relates to a crystalline form of nervonic acid and a preparation method thereof. In the X-ray powder diffraction pattern of the crystalline form, Cu-Kalpha radiation has characteristic peaks at diffraction angles 2theta of 5.2+ / -0.2, 8.8+ / -0.2, 12.4+ / -0.2, 16.0+ / -0.2, 19.2+ / -0.2, 23.9+ / -0.2 and 25.2+ / -0.2. The crystalline form provided by the application has good chemical purity and crystal stability, and exhibits excellent physical stability, which is beneficial to the development, production and storage of pharmaceutical preparations. In addition, research shows that the crystalline form also has suitable solubility characteristics, which is beneficial to improving the bioavailability and more beneficial to the related research and development of drugs.

This invention discloses a method for preparing EPA- and nervonic acid-enriched *Pteris vittata* oil, belonging to the field of fatty acid extraction technology. The method uses *Pteris vittata* as raw material, employing supercritical CO2 extraction to obtain crude oil, which is then enriched through enzymatic ester exchange and urea inclusion purification processes. Following solvent removal, decolorization, and deodorization, the EPA- and nervonic acid-enriched *Pteris vittata* oil is obtained. The *Pteris vittata* oil prepared by this invention contains ≥15g / 100g of EPA and ≥5g / 100g of nervonic acid, significantly promoting the synthesis and accumulation of phospholipids in the brain and exhibiting excellent repair and protection effects on nerve cells damaged by hydrogen peroxide. It can be widely used in the preparation of foods, health products, or pharmaceuticals that improve cognitive function, promote nerve repair, or maintain brain health.

The invention relates to a method for preparing a compound shown in a formula I. The method comprises the following steps: reducing a compound c to obtain a compound b; oxidizing the compound b to obtain the compound shown in the formula I. The method is mild in reaction condition, easy to control in operation, safe, reliable and low in cost, and can lay a foundation for later industrial enlarged production. Meanwhile, the preparation method disclosed by the invention is high in reaction yield, and the obtained product is high in purity.

This invention relates to an integrative plasmid for multicopy iteration of the Saccharomyces cerevisiae genome and its application, belonging to the field of biotechnology. This invention first constructs a multicopy integration vector, using Ty1, Ty2, Ty3, and Ty4 as multicopy integration target sites to construct four integrative plasmids. Each plasmid carries a fluorescent protein reporter gene (mScarlet-I or GFP) and an antibiotic resistance marker gene (such as hygromycin or genistein) to enable visualization and screening of the integration site. Applied to the preparation of nervonic acid, it achieves continuous editing without the need for monoclonal isolation. The strain obtained in the fourth round was screened by plate fermentation and verified in a 3 L fermenter, yielding a high nervonic acid production strain.

The invention discloses application of a triterpenoid saponin compound and nervonic acid in preparation of a medicine for preventing and treating Parkinson's disease and symptoms thereof. The triterpenoid saponin compounds and the nervonic acid in different proportions are combined, and experiments prove that multiple indexes of Parkinson mice can be remarkably improved when the triterpenoid saponin compounds and the nervonic acid are combined in a specific proportion, and the effect of synergistically improving the Parkinson disease or the progress of the Parkinson disease is achieved. Therefore, the pharmaceutical composition disclosed by the invention can be used for more effectively treating or improving the Parkinson's disease, and has a wide application prospect in the field of treatment of the Parkinson's disease.

1. Name of the product in this design: Label (Ampere Nervonic Acid Children's Growth Milk Powder). 2. Purpose of this design: For labeling. 3. The key design elements of this product are the combination of shape, pattern, and color. 4. The image or photograph that best illustrates the design's key points: the front view. 5. The design for which protection is sought includes color. 6. The back of this design product is a part that is not easily seen or cannot be seen during use, so the rear view is omitted; this design product is a thin product, so the left view, right view, top view, and bottom view are omitted.

1. Name of the designed product: packing box (Ganoderma lucidum nervonic acid tablet). 2. Use of the designed product: product packing box. 3. Design points of the designed product: combination of shape and pattern. 4. Picture or photo best indicating the design points: perspective view.