The invention relates to health-caring blend oil rich in alpha-linolenic acid and nervonic acid, as well as a detection method thereof. The health-caring blend oil rich in the alpha-linolenic acid and the nervonic acid comprises the following ingredients in parts by weight: 10-45 parts of linseed oil, 0-15 parts of deep-sea shark oil, 0-10 parts of sorbifolia oil, 5-45 parts of walnut oil, 0-15 parts of tomato seed oil, 0-35 parts of camellia oleifera seed oil, 0-15 parts of olive oil, 3-25 parts of perilla seed oil, and 1-8 parts of black sesame seed oil; and the characteristic fatty acid compositions and the contents thereof are detected by combining HS-SPME-GC-MS. The linseed oil, the deep-sea shark oil, the sorbifolia oil, the camellia oleifera seed oil, the walnut oil, the olive oil, the tomato seed oil, the perilla seed oil and the black sesame seed oil are combined in the blend oil so as to prepare the health-caring blend oil; the ingredients are rationally matched, and health-caring blend oil of different types can be blended according to dietary structures of different populations. The prepared health-caring blend oil is capable of supplementing amino acid, vitamins, minerals and trace elements needed by the human body, as well as keeping nutrition balance; moreover, the blended final products are good in taste, color and luster, and are capable of increasing appetite.