Device and method for separating nervonic acid from malania oleifera oil for biological medicine manufacturing

Abstract

The invention relates to the technical field of nervonic acid extraction, in particular to a device and method for separating nervonic acid from malania oleifera oil for biological medicine manufacturing, the device comprises a distiller, the distiller comprises a sealing tank and a dismounting cylinder, and a film scraping mechanism and a plurality of negative pressure tanks are arranged in the sealing tank and the dismounting cylinder respectively. According to the device and method for separating nervonic acid from malania oleifera oil for biological medicine manufacturing, a spiral scraping plate of a lifting part is linked with a reciprocating lead screw through a clamping ring, synchronous rotation and up-down reciprocating motion are achieved, the whole surface of a condensation pipe is covered, a condensate liquid film is actively scraped away, heat resistance of a retained liquid film is eliminated, the condensation efficiency is improved, and the efficiency of separation of nervonic acid from malania oleifera oil is improved. The nervonic acid fraction is prevented from staying on a condensation wall for a long time, and the condition of thermal degradation or backmixing is prevented.

Description

Technical Field

[0001] The present invention relates to the technical field of neuraminic acid extraction, and in particular to a device and method for separating neuraminic acid from garlic fruit oil for use in the manufacture of biopharmaceuticals. Background Art

[0002] Garlic fruit oil is a high-value biopharmaceutical raw material. Its core active ingredient, nervonic acid (tetracosenoic acid), has significant medicinal potential for the repair and development of the nervous system. However, nervonic acid has a large molecular weight, high heat sensitivity, and a boiling point close to that of other fatty acids. Traditional separation processes can easily lead to decomposition or insufficient purity. Molecular distiller is the key equipment to start large-scale production.

[0003] Patent application number CN202310137480.5 discloses a method for extracting neuraminic acid from Acer truncatum seeds. The neuraminic acid is extracted from Acer truncatum seed oil using an induced electric field assisted organic solvent. After extraction, the neuraminic acid in the Acer truncatum seed oil is extracted using molecular distillation and urea inclusion method. The induced electric field extraction can achieve gentle extraction without introducing exogenous substances, and the magnetic induction electric field can generate magnetic induction current. They each have the synergistic effect of non-thermal effect and thermal effect, accelerating the dissolution of Acer truncatum seed oil, thereby improving efficiency and yield.

[0004] The existing neuraminic acid separation device has significant bottlenecks: first, the static condensation system relies on gravity drainage, which causes high-viscosity neuraminic acid condensate to be retained on the pipe wall to form a liquid film, and the resulting thermal resistance causes fraction backmixing and thermal degradation; second, vacuum maintenance relies on the continuous operation of an external pump, and the overall equipment power consumption is high. When non-condensable gases accumulate at the condensation interface to form a gas boundary layer, the heat transfer efficiency will be further weakened.

[0005] In view of this, we propose an apparatus and method for separating neuraminic acid from garlic fruit oil for the manufacture of biopharmaceuticals. Summary of the Invention

[0006] The purpose of the present invention is to provide a device and method for separating nervonic acid from garlic fruit oil for the manufacture of biological medicines, realizing dual motion through a coaxially linked rotating part and a lifting part to solve the problems raised in the above-mentioned background technology.

[0007] To achieve the above objectives, the present invention provides the following technical solutions: A device for separating nervonic acid from garlic fruit oil for use in the manufacture of biopharmaceuticals comprises a distiller comprising a sealed tank and a disassembly cylinder, wherein a film scraping mechanism is provided inside the sealed tank and a plurality of negative pressure tanks are provided inside the disassembly cylinder; The film scraping mechanism includes a motor, a reciprocating screw driven by the motor, a rotating part arranged at the bottom end of the reciprocating screw, and a lifting part arranged inside the rotating part. The motor drives the reciprocating screw to drive the rotating part to rotate, thereby forming a thin film on the inner wall of the sealed tank. The lifting part includes a cylinder that rotates with the rotating part, a spiral scraper arranged on the inner wall of the cylinder, a number of round rods regularly distributed on the top of the cylinder, a pair of clamping plates, a clamping ring that rotates in the pair of clamping plates, and a limit strip arranged on the outer wall of the clamping ring. The reciprocating screw drives the clamping ring to move back and forth up and down, and synchronously drives the cylinder to move up and down on the rotating part; The negative pressure tank includes an air suction cylinder, a piston plate arranged inside the air suction cylinder, and a bending rod arranged on the top surface of the piston plate. When the limit bar moves with the clamping ring and collides with the bending rod, it drives it to move up together with the piston plate to extract the non-condensable gas in the sealed tank.

[0008] In the technical solution of the present invention, the distiller also includes a heating cylinder welded to the outer wall of the sealed tank, two liquid pipes connected by flanges to the upper and lower outer walls of the heating cylinder, and a liquid inlet pipe is flanged near the top of the outer wall of the sealed tank.

[0009] In the technical solution of the present invention, the disassembly cylinder is fixedly connected to the top of the sealed tank through a flange, a convex strip is provided on the inner wall of the disassembly cylinder, a sliding groove is provided on the side wall of the convex strip, and a plurality of regularly distributed openings are provided on the top surface of the disassembly cylinder.

[0010] In the technical solution of the present invention, the distiller also includes a sleeve welded and fixed to the bottom of the sealed tank, a partition welded and fixed between the sealed tank and the sleeve, a condenser fixedly connected to the inside of the sleeve through a flange, and a collecting bottle fixedly connected to the bottom end of the outer wall of the sleeve through a flange.

[0011] In the technical solution of the present invention, the motor is fixedly connected to the top surface of the disassembly cylinder by bolts, and the reciprocating screw is coaxially connected to the output shaft of the motor.

[0012] In the technical solution of the present invention, the rotating part includes a number of regularly distributed fixing rings, a number of reinforcing strips welded and fixed between the fixing rings, a scraper strip fixedly connected to the outer groove of the reinforcing strip by screws, and a liquid guide cover fixedly connected to the top surface of the top fixing ring by screws. The bottom end of the reciprocating screw is fixedly connected to the top surface of the liquid guide cover by bolts.

[0013] In the technical solution of the present invention, the cylinder is sleeved on the outer wall of the condenser, the spiral scraper is welded and fixed on the inner wall of the cylinder, the bottom end of the round rod is clamped and fixed on the top surface of the cylinder and the top end extends to the top surface of the liquid guide cover.

[0014] In the technical solution of the present invention, the two clamping plates are fixedly connected by bolts, and a clamping ring is left between them to provide a rotation range for the clamping ring. The clamping plate located below is clamped and fixed to the top of several round rods. The clamping ring is rotatably connected between the two clamping plates and is sleeved on the outside of the reciprocating screw rod. The limit bar is clamped and fixed on the outer wall of the clamping ring, and the other end is slidably connected to the inside of the slide groove.

[0015] In the technical solution of the present invention, the suction cylinder is clamped and fixed on the circular ring on the inner bottom surface of the disassembly cylinder, and the one-way air inlet valve and the air outlet valve are respectively clamped and fixed on the bottom surface and the outer wall near the bottom of the suction cylinder. The piston plate is slidably connected to the interior of the suction cylinder, and a spring is provided on the outer side of the piston plate. The elastic force provided by the spring pushes the piston plate downward, and the longitudinal cross-section of the bent rod is an inverted L-shape.

[0016] On the other hand, the present invention also provides a method for separating nervonic acid from garlic fruit oil for use in biopharmaceutical production, using the above-mentioned device for separating nervonic acid from garlic fruit oil for use in biopharmaceutical production, comprising the following steps: S1. First, the garlic fruit oil is alkali-refined, degummed and deacidified, and then vacuum-dried to prevent high-temperature hydrolysis. The garlic fruit oil is then reacted with methanol under the catalysis of sodium methoxide at 70° C.-80° C. for 1-1.5 hours to produce fatty acid methyl esters. S2. Then, the air inside the sealed tank is evacuated by an external vacuum device, and the generated solution is sent into the sealed tank through the liquid inlet pipe. The heat-conducting solution is sent into the sealed tank through the liquid pipe at the bottom end of the heating cylinder and then drawn out from the liquid pipe at the top, thereby increasing the internal temperature of the sealed tank; S3. At this time, the motor in the film scraping mechanism is turned on to drive the reciprocating screw to rotate, which in turn drives the rotating part fixed at its bottom end to rotate as a whole. The solution flows evenly through the liquid guide cover to the inner wall of the sealed tank and passes through the scraping bar, so that the solution forms a thin film on the inner wall of the sealed tank. S4. Subsequently, as the solution falls under its own gravity, after passing through the heating cylinder area, the light components in the solution are evaporated by the heat, pass through the rotating part, and are released to the outer wall of the condenser tube, where they are re-condensed into liquid form. The heavy components fall along the tube wall of the sealed tank and are guided by the partition to the collection bottle located above. S5. During the distillation process, the cylinder of the lifting part and the internal spiral scraper rotate synchronously with the rotating part, continuously scraping off the liquid product formed on the surface of the condenser tube and guiding it downward along the sleeve for collection; S6. At the same time, the clamping ring is driven by the reciprocating screw to drive the cylinder to reciprocate up and down in the disassembly cylinder, so that the spiral scraper covers the entire surface of the condenser tube during the rotation process, and the solution formed by the light component flows from the bottom of the sleeve to the collection bottle located below; S7. During the upward movement of the clamp ring, it comes into conflict with the horizontal bar of the bent rod, thereby driving the bent rod and the piston plate to move upward inside the suction cylinder, thereby extracting the non-condensable gas in the sealed tank through the suction valve at the bottom of the suction cylinder during the distillation process; S8. Afterwards, when the primary distillation operation is completed, the operator collects the liquid in the upper collecting bottle and introduces it into another distiller. After the distillation is completed, the operator collects the light component solution in the lower collecting bottle and cools it down in an acetone solvent in a gradient manner. The distillate is dissolved and crystallized, and the saturated fatty acids are removed by filtration, thereby obtaining high-purity neuraminic acid. Compared with the prior art, the present invention has the following beneficial effects: 1. The device and method for separating neuraminic acid from garlic fruit oil for the manufacture of biopharmaceuticals, the spiral scraper of the lifting part is linked to the reciprocating screw through a clamping ring to achieve synchronous rotation and up and down reciprocating motion, covering the entire surface of the condenser tube, actively scraping off the condensation liquid film to eliminate the thermal resistance of the retained liquid film, improve the condensation efficiency, avoid the neuraminic acid fraction staying on the condenser wall for too long, and prevent thermal degradation or backmixing.

[0017] 2. The device and method for separating neuraminic acid from garlic fruit oil for the manufacture of biopharmaceuticals utilize the reciprocating motion of the lifting part to drive the limit bar to periodically contact the bending rod, driving the piston plate to form air extraction in the negative pressure tank. The spring resets to form a closed cycle, allowing the external vacuum pump to only establish an initial vacuum, shortening its operating time and reducing power consumption. It also eliminates the thermal boundary layer of the gas on the surface of the condenser tube, reducing heat transfer resistance and further improving condensation efficiency. BRIEF DESCRIPTION OF THE DRAWINGS

[0018] Figure 1 It is a schematic diagram of the overall structure of the present invention; Figure 2 It is a schematic cross-sectional view of the overall structure of the present invention; Figure 3 It is a schematic cross-sectional view of the structure of the distiller of the present invention; Figure 4 It is a partial structural schematic diagram of the distiller of the present invention; Figure 5 It is a schematic cross-sectional view of the structure of the disassembly cylinder in the present invention; Figure 6 Schematic diagram of the structure of the film scraping mechanism in the present invention; Figure 7 Schematic diagram of the structure of the rotating part of the present invention; Figure 8 It is a schematic cross-sectional view of the structure of the lifting part of the present invention; Figure 9 It is a schematic cross-sectional view of the structure of the negative pressure tank in the present invention; Description of reference numerals: 100, distiller; 110, sealed tank; 120, heating cylinder; 130, liquid pipe; 140, liquid inlet pipe; 150, disassembly cylinder; 151, chute; 160, sleeve; 170, partition; 180, condenser; 190, collecting flask; 200, scraping mechanism; 210, motor; 220, reciprocating screw; 230, rotating part; 231, fixing ring; 232, reinforcing strip; 233, scraping strip; 234, liquid guide cover; 240, lifting part; 241, cylinder; 242, spiral scraper; 243, round rod; 244, clamping plate; 245, clamping ring; 246, limit strip; 300. Negative pressure tank; 310. Suction cylinder; 320. Piston plate; 330. Spring; 340. Bending rod. DETAILED DESCRIPTION

[0019] The following will provide a clear and complete description of the technical solutions of the present invention in conjunction with the accompanying drawings. Obviously, the embodiments described are only some of the embodiments of the present invention, not all of them. All other embodiments obtained by ordinary technicians in this field based on the embodiments of the present invention without making any creative efforts shall fall within the scope of protection of the present invention. See also Figure 1-Figure 5 As shown, this embodiment provides a technical solution: The device for separating nervonic acid from garlic fruit oil for the manufacture of biological medicines includes a distiller 100, which includes a disassembly cylinder 150 and a sealing tank 110 coaxially connected in an upper and lower direction. A scraping mechanism 200 is provided inside the sealing tank 110, and a plurality of negative pressure tanks 300 are provided inside the disassembly cylinder 150.

[0020] Specifically, the distiller 100 also includes a heating tube 120 welded to the outer wall of the sealed tank 110, two liquid pipes 130 connected by flanges to the upper and lower outer walls of the heating tube 120, and a liquid inlet pipe 140 connected by a flange near the top of the outer wall of the sealed tank 110.

[0021] Furthermore, the disassembly cylinder 150 is fixedly connected to the top of the sealing tank 110 through a flange. A convex strip is provided on the inner wall of the disassembly cylinder 150 , and a sliding groove 151 is provided on the side wall of the convex strip. A plurality of regularly distributed openings are provided on the top surface of the disassembly cylinder 150 .

[0022] Furthermore, the distiller 100 also includes a sleeve 160 welded and fixed to the bottom of the sealed tank 110, a partition 170 welded and fixed between the sealed tank 110 and the sleeve 160, a condenser 180 fixedly connected to the inside of the sleeve 160 through a flange, and a collecting bottle 190 fixedly connected to the bottom end of the outer wall of the sleeve 160 through a flange.

[0023] Furthermore, the sealing tank 110 is used to ensure that the distiller 100 cooperates with the disassembly cylinder 150 to ensure the internal airtightness. When in use, the air inside the sealing tank 110 is first extracted through an external vacuum device, and the generated solution is sent into the interior of the sealing tank 110 through the liquid inlet pipe 140, and the heat-conducting solution is sent in from the liquid pipe 130 at the bottom end of the heating cylinder 120 and then drawn out from the liquid pipe 130 at the top, thereby increasing the internal temperature of the sealing tank 110, and the slide groove 151 in the disassembly cylinder 150 is used to limit the moving range of the internal structure of the scraping mechanism 200, the sleeve 160 is used to collect the liquid condensed from the outer wall of the condenser 180, and the partition 170 is used to guide the liquid flowing down from the inner wall of the sealing tank 110 into the collecting bottle 190. This setting allows the structure inside the scraping mechanism 200 to have sufficient lifting space in a sealed state through the design of the disassembly cylinder 150.

[0024] See also Figure 6-Figure 7 As shown, in this embodiment, the film scraping mechanism 200 includes a motor 210, a reciprocating screw 220 driven by the motor 210, a rotating part 230 arranged at the bottom end of the reciprocating screw 220, and a lifting part 240 arranged inside the rotating part 230. The motor 210 drives the reciprocating screw 220 to drive the rotating part 230 to rotate, thereby forming a thin film on the inner wall of the sealed can 110.

[0025] Specifically, the motor 210 is fixedly connected to the top surface of the disassembly cylinder 150 by bolts, and the reciprocating screw 220 is coaxially connected to the output shaft of the motor 210 .

[0026] Furthermore, the rotating part 230 includes a number of regularly distributed fixing rings 231, a number of reinforcing strips 232 welded and fixed between the fixing rings 231, a scraper strip 233 fixedly connected to the outer groove of the reinforcing strip 232 by screws, and a liquid guide cover 234 fixedly connected to the top surface of the top fixing ring 231 by screws. The bottom end of the reciprocating screw 220 is fixedly connected to the top surface of the liquid guide cover 234 by bolts.

[0027] Furthermore, the motor 210 in the scraping mechanism 200 is turned on to drive the reciprocating screw 220 to rotate, thereby driving the rotating part 230 fixed at its bottom end to rotate as a whole. The solution flows evenly to the inner wall of the sealed tank 110 through the liquid guide cover 234, and forms a thin film on the inner wall of the sealed tank 110 through the scraping bar 233. This setting drives the rotating part 230 to rotate through the reciprocating screw 220, and provides conditions for the subsequent up and down movement of the lifting part 240 inside the rotating part 230.

[0028] See also Figure 6-Figure 8As shown, in this embodiment, the lifting part 240 includes a cylinder 241 that rotates with the rotating part 230, a spiral scraper 242 arranged on the inner wall of the cylinder 241, a number of round rods 243 regularly distributed on the top of the cylinder 241, a pair of clamping plates 244, a clamping ring 245 rotating in the pair of clamping plates 244, and a limiting strip 246 arranged on the outer wall of the clamping ring 245. The reciprocating screw 220 drives the clamping ring 245 to move back and forth up and down, and synchronously drives the cylinder 241 to move up and down on the rotating part 230.

[0029] Specifically, the cylinder 241 is sleeved on the outer wall of the condenser 180, the spiral scraper 242 is welded and fixed on the inner wall of the cylinder 241, the bottom end of the round rod 243 is clamped and fixed on the top surface of the cylinder 241 and the top end extends to the top surface of the liquid guide cover 234.

[0030] Furthermore, the two clamping plates 244 are fixedly connected by bolts, and a clamping ring 245 is left between them to provide a rotation range for the clamping ring 245. The clamping plate 244 located at the bottom is clamped and fixed to the top of several round rods 243. The clamping ring 245 is rotatably connected between the two clamping plates 244 and is sleeved on the outside of the reciprocating screw rod 220. The limit bar 246 is clamped and fixed on the outer wall of the clamping ring 245, and the other end is slidably connected to the inside of the slide groove 151.

[0031] Furthermore, during the distillation process, the cylinder 241 of the lifting part 240 and the internal spiral scraper 242 rotate synchronously with the rotating part 230, continuously scraping off the liquid product formed on the surface of the condenser tube 180, and guiding it downward along the sleeve 160 for collection. At the same time, the clamping ring 245, driven by the reciprocating screw 220, drives the cylinder 241 to reciprocate up and down in the disassembly cylinder 150, so that the spiral scraper 242 covers the entire surface of the condenser tube 180 during the rotation process, and allows the solution formed by the light component to flow from the bottom of the sleeve 160 to the collection bottle 190 located below. This setting actively scrapes off the condensation liquid film through the cylinder 241 and the spiral scraper 242, thereby eliminating the thermal resistance of the retained liquid film and improving the condensation efficiency.

[0032] See also Figure 9 As shown, in this embodiment, the negative pressure tank 300 includes an air suction cylinder 310, a piston plate 320 arranged inside the air suction cylinder 310, and a bending rod 340 arranged on the top surface of the piston plate 320. The limiting strip 246 moves with the clamping ring 245 and collides with the bending rod 340, driving it to move upward together with the piston plate 320 to extract the non-condensable gas in the sealed tank 110.

[0033] Specifically, the air suction cylinder 310 is clamped and fixed on the circular ring on the inner bottom surface of the disassembly cylinder 150, and the one-way air inlet valve and the air outlet valve are clamped and fixed on the bottom surface and the outer wall near the bottom of the air suction cylinder 310 respectively. The piston plate 320 is slidably connected to the inside of the air suction cylinder 310, and a spring 330 is provided on the outer side of the piston plate 320. The elastic force provided by the spring 330 pushes the piston plate 320 to move downward, and the longitudinal cross-section of the bending rod 340 is an inverted L shape.

[0034] Furthermore, during the upward movement of the clamping ring 245, it conflicts with the cross bar of the bending rod 340, thereby driving the bending rod 340 and the piston plate 320 to move upward inside the suction cylinder 310, and then extracting the non-condensable gas in the sealed tank 110 through the suction valve at the bottom of the suction cylinder 310 during the distillation process. This setting removes the non-condensable gas in real time, allowing the external vacuum pump to only establish an initial vacuum, shortening its operating time, and eliminating the thermal boundary layer of the gas on the surface of the condenser 180, thereby reducing the heat transfer resistance and further improving the condensation efficiency. The present invention also provides a method for separating nervonic acid from garlic fruit oil for use in biopharmaceutical production. The method uses the above-mentioned device for separating nervonic acid from garlic fruit oil for use in biopharmaceutical production, comprising the following steps: S1. First, the garlic fruit oil is alkali-refined, degummed and deacidified, and then vacuum-dried to prevent high-temperature hydrolysis. The garlic fruit oil is then reacted with methanol under the catalysis of sodium methoxide at 70° C.-80° C. for 1-1.5 hours to produce fatty acid methyl esters. S2. Next, the air inside the sealed tank 110 is evacuated by an external vacuum device, and the generated solution is fed into the sealed tank 110 through the liquid inlet pipe 140. The heat-conducting solution is fed into the sealed tank 110 through the liquid pipe 130 at the bottom end of the heating cylinder 120 and then drawn out from the liquid pipe 130 at the top, thereby increasing the internal temperature of the sealed tank 110. S3. At this time, the motor 210 in the film scraping mechanism 200 is turned on to drive the reciprocating screw 220 to rotate, which in turn drives the rotating part 230 fixed at its bottom end to rotate as a whole. The solution flows evenly toward the inner wall of the sealed tank 110 through the liquid guide cover 234 and forms a thin film on the inner wall of the sealed tank 110 through the scraping bar 233. S4. Subsequently, the solution falls under its own gravity, passes through the heating cylinder 120 area, and the light components in the solution are evaporated by the heat. After passing through the rotating part 230, they are released to the outer wall of the condenser tube 180, where they are re-condensed into liquid. The heavy components fall along the tube wall of the sealed tank 110 and are guided by the partition 170 to the collection bottle 190 located above. S5. During the distillation process, the cylinder 241 of the lifting portion 240 and the internal spiral scraper 242 rotate synchronously with the rotating portion 230, continuously scraping off the liquid product formed on the surface of the condenser tube 180 and guiding it downward along the sleeve 160 for collection; S6. At the same time, the clamp ring 245, driven by the reciprocating screw 220, drives the cylinder 241 to reciprocate up and down in the disassembly cylinder 150, so that the spiral scraper 242 covers the entire surface of the condenser tube 180 during the rotation process, and the solution formed by the light component flows from the bottom of the sleeve 160 to the collection bottle 190 located below; S7: During the upward movement of the clamp ring 245, it interferes with the crossbar of the bent rod 340, thereby driving the bent rod 340 and the piston plate 320 to move upward inside the suction cylinder 310, thereby extracting the non-condensable gas in the sealed tank 110 through the suction valve at the bottom of the suction cylinder 310 during the distillation process; S8. Afterwards, when the primary distillation operation is completed, the operator collects the liquid in the upper collecting bottle 190 and introduces it into another distiller 100. After the distillation is completed, the operator collects the light component solution in the lower collecting bottle 190, and cools it in an acetone solvent in a gradient manner. The distillate is dissolved and crystallized, and the saturated fatty acids are filtered to remove them, thereby obtaining high-purity neuraminic acid. The foregoing descriptions of specific exemplary embodiments of the present invention are for purposes of illustration and description. These descriptions are not intended to limit the invention to the precise forms disclosed, and it is apparent that many variations and modifications are possible in light of the foregoing teachings. The exemplary embodiments have been selected and described for the purpose of explaining the specific principles of the invention and their practical application, thereby enabling those skilled in the art to make and utilize a variety of exemplary embodiments of the invention and various options and modifications. The scope of the invention is intended to be defined by the description and its equivalents.

Claims

1. A device for separating nervonic acid from garlic fruit oil for use in the manufacture of biopharmaceuticals, comprising a distiller comprising a sealed tank and a disassembly cylinder, characterized in that: The sealing tank is equipped with a scraping mechanism inside, and the disassembly cylinder is equipped with several negative pressure tanks inside; The film scraping mechanism includes a motor, a reciprocating screw driven by the motor, a rotating part arranged at the bottom end of the reciprocating screw, and a lifting part arranged inside the rotating part. The motor drives the reciprocating screw to drive the rotating part to rotate, thereby forming a thin film on the inner wall of the sealed tank. The lifting part includes a cylinder that rotates with the rotating part, a spiral scraper arranged on the inner wall of the cylinder, a number of round rods regularly distributed on the top of the cylinder, a pair of clamping plates, a clamping ring that rotates in the pair of clamping plates, and a limit strip arranged on the outer wall of the clamping ring. The reciprocating screw drives the clamping ring to move back and forth up and down, and synchronously drives the cylinder to move up and down on the rotating part; The negative pressure tank includes an air suction cylinder, a piston plate arranged inside the air suction cylinder, and a bending rod arranged on the top surface of the piston plate. When the limit bar moves with the clamping ring and collides with the bending rod, it drives it to move up together with the piston plate to extract the non-condensable gas in the sealed tank.

2. The device for separating nervonic acid from garlic fruit oil for manufacturing biopharmaceuticals according to claim 1, characterized in that: The distiller also includes a heating cylinder welded to the outer wall of the sealed tank, two liquid pipes connected by flanges to the upper and lower outer walls of the heating cylinder, and a liquid inlet pipe is connected by flanges at a position near the top of the outer wall of the sealed tank.

3. The device for separating nervonic acid from garlic fruit oil for manufacturing biopharmaceuticals according to claim 2, wherein: The disassembly cylinder is fixedly connected to the top of the sealed tank through a flange. A convex strip is arranged on the inner wall of the disassembly cylinder, a sliding groove is opened on the side wall of the convex strip, and a plurality of regularly distributed openings are opened on the top surface of the disassembly cylinder.

4. The device for separating nervonic acid from garlic fruit oil for manufacturing biopharmaceuticals according to claim 3, characterized in that: The distiller also includes a sleeve welded to the bottom of the sealed tank, a partition welded between the sealed tank and the sleeve, a condenser fixedly connected to the inside of the sleeve via a flange, and a collecting bottle fixedly connected to the bottom end of the outer wall of the sleeve via a flange.

5. The device for separating nervonic acid from garlic fruit oil for manufacturing biopharmaceuticals according to claim 4, characterized in that: The motor is fixedly connected to the top surface of the disassembly cylinder by means of bolts, and the reciprocating screw is coaxially connected to the output shaft of the motor.

6. The device for separating nervonic acid from garlic fruit oil for manufacturing biopharmaceuticals according to claim 5, characterized in that: The rotating part includes several regularly distributed fixing rings, several reinforcing strips welded and fixed between the several fixing rings, scraping strips fixedly connected to the outer grooves of the reinforcing strips by screws, and a liquid guide cover fixedly connected to the top surface of the top fixing ring by screws. The bottom end of the reciprocating screw is fixedly connected to the top surface of the liquid guide cover by bolts.

7. The device for separating nervonic acid from garlic fruit oil for manufacturing biopharmaceuticals according to claim 6, characterized in that: The cylinder is sleeved on the outer wall of the condenser tube, the spiral scraper is welded and fixed on the inner wall of the cylinder, the bottom end of the round rod is clamped and fixed on the top surface of the cylinder and the top end extends to the top surface of the liquid guide cover.

8. The device for separating nervonic acid from garlic fruit oil for manufacturing biopharmaceuticals according to claim 7, characterized in that: The two clamping plates are fixedly connected by bolts, and a clamping ring is left between them to provide a rotation range for the clamping ring. The clamping plate located below is clamped and fixed to the top of several round rods. The clamping ring is rotatably connected between the two clamping plates and is sleeved on the outside of the reciprocating screw rod. The limit bar is clamped and fixed on the outer wall of the clamping ring, and the other end is slidably connected to the inside of the slide groove.

9. The device for separating nervonic acid from garlic fruit oil for manufacturing biopharmaceuticals according to claim 8, characterized in that: The suction cylinder is clamped and fixed on the circular ring on the inner bottom surface of the disassembly cylinder. The bottom surface of the suction cylinder and the outer wall near the bottom are respectively clamped and fixed with a one-way air inlet valve and an air outlet valve. The piston plate is slidably connected to the inside of the suction cylinder. A spring is provided on the outer side of the piston plate. The elastic force provided by the spring pushes the piston plate downward. The longitudinal cross-section of the bent rod is an inverted L-shape.

10. A method for separating nervonic acid from garlic fruit oil for use in biopharmaceutical production, using the apparatus for separating nervonic acid from garlic fruit oil for use in biopharmaceutical production according to claim 9, characterized in that: The following steps are involved: S1. First, the garlic fruit oil is alkali-refined, degummed and deacidified, and then vacuum-dried to prevent high-temperature hydrolysis. The garlic fruit oil is then reacted with methanol under the catalysis of sodium methoxide at 70° C.-80° C. for 1-1.5 hours to produce fatty acid methyl esters. S2. Then, the air inside the sealed tank is evacuated by an external vacuum device, and the generated solution is sent into the sealed tank through the liquid inlet pipe. The heat-conducting solution is sent into the sealed tank through the liquid pipe at the bottom end of the heating cylinder and then drawn out from the liquid pipe at the top, thereby increasing the internal temperature of the sealed tank; S3. At this time, the motor in the film scraping mechanism is turned on to drive the reciprocating screw to rotate, which in turn drives the rotating part fixed at its bottom end to rotate as a whole. The solution flows evenly through the liquid guide cover to the inner wall of the sealed tank and passes through the scraping bar, so that the solution forms a thin film on the inner wall of the sealed tank. S4. Subsequently, as the solution falls under its own gravity, after passing through the heating cylinder area, the light components in the solution are evaporated by the heat, pass through the rotating part, and are released to the outer wall of the condenser tube, where they are re-condensed into liquid form. The heavy components fall along the tube wall of the sealed tank and are guided by the partition to the collection bottle located above. S5. During the distillation process, the cylinder of the lifting part and the internal spiral scraper rotate synchronously with the rotating part, continuously scraping off the liquid product formed on the surface of the condenser tube and guiding it downward along the sleeve for collection; S6. At the same time, the clamping ring is driven by the reciprocating screw to drive the cylinder to reciprocate up and down in the disassembly cylinder, so that the spiral scraper covers the entire surface of the condenser tube during the rotation process, and the solution formed by the light component flows from the bottom of the sleeve to the collection bottle located below; S7. During the upward movement of the clamp ring, it comes into conflict with the horizontal bar of the bent rod, thereby driving the bent rod and the piston plate to move upward inside the suction cylinder, thereby extracting the non-condensable gas in the sealed tank through the suction valve at the bottom of the suction cylinder during the distillation process; S8. Afterwards, when the primary distillation operation is completed, the operator collects the liquid in the upper collecting bottle and introduces it into another distiller. After the distillation is completed, the operator collects the light component solution in the lower collecting bottle and cools it down in an acetone solvent in a gradient manner. The distillate is dissolved and crystallized, and the saturated fatty acids are removed by filtration, thereby obtaining high-purity neuraminic acid.

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