842 results about "Macroporous resin" patented technology

The invention discloses a method for preparing, enriching and separating various egg yolk bioactive peptides through enzymolysis of defatted egg yolk powder and application of the egg yolk bioactive peptides, and belongs to the technical field of egg product processing and bioactive peptides. Egg yolk protein hydrolysate is prepared by adopting a three-step enzymolysis process, adsorption and gradient elution are performed on the egg yolk protein hydrolysate by adopting a macroporous resin chromatographic column, four components including water-1, water-2, 20% ethanol and 80% ethanol are obtained, the water-1 component is common egg yolk peptide, the water-1 component is enriched with osteogenic active peptide, the water-2 component is enriched with ethanol, and the 80% ethanol is enriched with ethanol. The 20% ethanol component enriches antioxidant peptide, and the 80% ethanol component enriches ACE inhibitory peptide. The functional activity of each bioactive peptide component is very strong, and the bioactive peptide component can be used as a high-end functional ingredient. According to the method disclosed by the invention, comprehensive utilization of the degreased yolk powder is realized, enrichment and separation of three active peptides are realized by using one raw material through a step-by-step enzymolysis-directional separation technology, the yield of the active peptides is greatly improved, and the economic benefits of enterprises are improved.

The invention discloses purified gastrodia elata polysaccharide as well as a preparation method and application thereof, and belongs to the technical field of extraction of active ingredients of gastrodia elata. The method comprises the following steps: (1) adding an alcoholic solution into gastrodia elata powder for reflux extraction, and collecting filter residues; (2) dissolving the filter residues in pure water, and performing ultrasonic extraction to obtain filtrate; (3) adding absolute ethyl alcohol into the filtrate for low-temperature alcohol precipitation to obtain crude gastrodia elata polysaccharide; (4) performing deproteinization treatment on the gastrodia elata crude polysaccharide; (5) purifying by using macroporous resin; (6) dialyzing the crude polysaccharide purified liquid in pure water; (7) purifying by using a first chromatographic column, wherein a filler is cellulose-based anion exchange resin; (8) purifying by using a second chromatographic column to obtain purified gastrodia elata polysaccharide; and the filler is sephadex. According to the present invention, the gastrodia elata crude polysaccharide is purified by using the five purification means, such that the high-purity gastrodia elata purified polysaccharide can be rapidly, stably and efficiently obtained.

The invention discloses a plant-source nutritional supplement based on glycoside compounds and a preparation method thereof, and relates to the technical field of preparation of plant-source nutritional supplements.The preparation method comprises the steps that raw materials are prepared; the preparation method comprises the following steps: respectively carrying out ultrasonic-assisted extraction on flavonoid glycoside, saponin and geniposide raw materials to obtain an extracting solution; carrying out centrifugal separation on the extracting solution, taking supernate, and then purifying by adopting a macroporous resin adsorption method to obtain a purified glycoside compound solution; mixing the purified glycoside compound solution with lycium barbarum polysaccharide, Chinese yam starch, vitamin E and other auxiliaries in proportion, and uniformly stirring to form a mixed solution; carrying out spray drying on the mixed solution to obtain a powdery nutritional supplement; and screening the powdery nutritional supplement. The high-purity plant source glycoside compound and the functional auxiliary agent are scientifically compounded, and ultrasonic extraction, resin purification and precise process control are combined, so that the integrated improvement of activity synergy, quality stability and personalized scene adaptation of the nutritional supplement is realized.

The invention discloses a method for extracting and purifying flavone in litsea coreana through an ultrasonic-assisted eutectic solvent, and belongs to the technical field of green processing of natural products. The invention provides a method for efficiently preparing litsea coreana flavone by integrating computer prediction, green solvent extraction and resin purification technologies. The method comprises the following steps: firstly, calculating and screening a deep eutectic solvent system with the highest affinity with the litsea coreana flavone on the basis of a COSMO-RS theoretical model, then coupling the optimized DES with an ultrasonic-assisted extraction (UAE) technology, and through the synergistic effect of the DES and the UAE technology, remarkably improving the extraction rate of the litsea coreana flavone and furthest keeping the biological activity of a target component; then, a macroporous resin dynamic adsorption-desorption purification process is linked, so that high-selectivity enrichment and impurity removal of the flavonoid compounds in the crude extract are realized, a high-purity product is obtained, and a complete process route from intelligent screening, efficient extraction to precise purification is finally formed.

The invention provides an adsorbent capable of simultaneously adsorbing bilirubin and cytokines and a preparation method and application thereof, and the preparation method comprises the following steps: carrying out suspension polymerization reaction on a styrene monomer and a polyvinyl cross-linking agent in a dispersion medium under the action of a continuous multi-component pore-foaming agent and an initiator to prepare polystyrene-based macroporous resin; carrying out chloromethylation reaction on the polystyrene-based macroporous resin to prepare chlorine balls; carrying out post-crosslinking reaction on the chloromethylated beads to prepare the adsorbent capable of adsorbing bilirubin and cytokines at the same time; the continuous multi-component pore-foaming agent comprises a first pore-foaming agent, a second pore-foaming agent and a third pore-foaming agent of which the solubility parameters are gradually increased in sequence; the temperature of the suspension polymerization reaction is adjusted according to the mass fraction of the polyvinyl cross-linking agent in the monomer mixture and the solubility parameter of the continuous multi-component pore-foaming agent, and the monomer mixture is composed of styrene monomers and the polyvinyl cross-linking agent. The bilirubin and cell factors can be adsorbed at the same time, and the safety of blood purification is improved.

The invention relates to the technical field of elastin peptides, in particular to a method for extracting elastin peptides from animal cartilage, which comprises the following specific steps: removing surface connective tissues and fat from animal cartilage, cleaning with normal saline, grinding and degreasing to obtain pretreated cartilage particles; putting the pretreated cartilage particles into a container, adding ionic liquid and stirring; after stirring is completed, centrifugally collecting precipitates; adding the precipitate into a phosphate buffer solution to form a suspension; adding an immobilized compound enzyme, and carrying out an enzymolysis reaction; after the enzymolysis is completed, raising the temperature for inactivation; filtering to obtain enzymatic hydrolysate; adding macroporous resin into the enzymatic hydrolysate, and filtering to obtain clear liquid; and carrying out ultrafiltration on the clear liquid to obtain the elastin peptide. The ionic liquid interacts with impurities in the animal cartilage, so that the impurities are easier to remove, a purer raw material environment is created for subsequent elastin peptide extraction, and the extraction efficiency is improved.

The invention discloses an enantiokaurane compound with antitumor activity as well as a preparation method and application of the enantiokaurane compound. According to the present invention, chemical components of Western African plant I.trichantha tubers are deeply studied, and a variety of chromatographic separation methods such as macroporous resin, silica gel resin, medium pressure preparation-MCI column, gel normal pressure column and semi-preparative high performance liquid chromatography are combined to separate to obtain one enantiokaurane new compound Trichanthone B; experiments show that the compound obtained through separation has a good inhibition effect on an MIA PaCa-2 pancreatic cancer cell line and an A549 lung cancer cell line, and IC50 of the compound on the MIA PaCa-2 pancreatic cancer cell line reaches a nanomole level; the effect of the compound is stronger than that of positive drugs 5-fluorouracil and carboplatin, and the compound has the potential of being developed into new drugs for resisting pancreatic cancer and lung cancer.

The invention discloses a radix pseudostellariae extract with high content of radix pseudostellariae cyclic peptide B and a preparation method and cosmetic application of the radix pseudostellariae extract, and belongs to the technical field of cosmetics. The preparation method comprises the following steps: (1) crushing radix pseudostellariae, adding alcohol and performing ultrasonic treatment to obtain feed liquid; (2) adding a compound enzyme into the feed liquid to carry out enzymolysis, enzyme deactivation, solid-liquid separation, concentration and impurity removal to obtain an enzymatic hydrolysate; and (3) eluting the enzymatic hydrolysate with macroporous resin to obtain an eluent, and drying to obtain the product, the compound enzyme is prepared from pectinase, papain, beta-glucosidase, cellulase and xylanase according to the mass ratio of (5 to 10) to (0.5 to 1) to (3 to 7) to (0.3 to 1). The radix pseudostellariae extract disclosed by the invention is prepared by adopting an enzymolysis process, more active ingredients can be reserved, the content of radix pseudostellariae cyclic peptide B in the obtained extract is high, and the radix pseudostellariae extract has relatively good anti-inflammatory, soothing, repairing and anti-aging effects.

The invention relates to the field of cosmetics, in particular to a berry composition for scalp care and a preparation method and application thereof, and the preparation method comprises the following steps: A, pretreating fruits to obtain berry freeze-dried powder; b, preparing a deep eutectic solvent; c, mixing the berry freeze-dried powder with the deep-eutectic solvent, and performing oscillation extraction to obtain a berry procyanidine crude extract; d, purifying the berry procyanidine crude extract with macroporous resin to obtain a first purified berry procyanidine product; e, removing the solvent from the first purified berry proanthocyanidins to obtain a second purified berry proanthocyanidins; and F, dissolving epicatechin, mixing the dissolved epicatechin with the second purified product of the berry procyanidine, uniformly heating in a vacuum environment, cooling after relieving the vacuum environment, mixing with amino acid hydrochloride, and preserving heat to obtain the berry extract.

The invention discloses emblic leafflower fruit polysaccharide with sugar-controlling and lipid-lowering activity as well as a preparation method and application of the emblic leafflower fruit polysaccharide. The polysaccharide is prepared by taking fresh phyllanthus emblica pulp as a raw material through hot water extraction, yeast fermentation, desorption after XAD-16 macroporous resin adsorption, alcohol precipitation, ultrafiltration and DEAE-52 cellulose column purification. The low-molecular-weight gulcomannan is separated from phyllanthus emblica for the first time, and monosaccharide composition of the gulcomannan mainly comprises glucose, mannose and galactose and contains trace rhamnose and xylose. The polysaccharide has sugar-controlling and lipid-lowering active action targets in the whole digestive tract (the upper digestive tract: glycolipid digestion and absorption enzymes alpha-amylase, alpha-glucosidase and pancreatic lipase inhibitory activity, starch hydrolysis delay, bile acid adsorption capacity and cholesterol micelle dissolution inhibition capacity; in the lower digestive tract, the multiplication promoting capacity of glycolipid metabolism-related probiotics) shows extremely high biological activity, and can be applied to food and health care products.

The invention discloses a macroporous resin material and a preparation method thereof, and macroporous resin with high specific surface area and excellent adsorption performance is obtained through a specific modified acrylate polymeric monomer and a preparation method of the macroporous resin material. The method comprises the following steps: reacting quercetin, methyl vanillate and acryloyl chloride to prepare a modified acrylate polymeric monomer, mixing the modified acrylate polymeric monomer with polyvinyl alcohol, ethylene glycol dimethacrylate, dodecanol, benzoyl peroxide and the like, and carrying out suspension polymerization to prepare the macroporous resin material. The prepared macroporous resin material has excellent performance in the aspects of pore structure, polarity and the like, can be widely applied to the related fields of adsorption separation and the like, and provides a new thought and method for research and development of the macroporous resin material.

The invention provides a percolation device and a preparation method of a phyllanthus emblica extract. The percolation device comprises a percolation tank, a pressing device and a plurality of separation structures, the preparation method of the emblic leafflower fruit extract comprises the following steps: firstly, coarsely crushing and soaking an emblic leafflower fruit medicinal material, then percolating the emblic leafflower fruit medicinal material in a percolating device, and collecting percolate; the preparation method comprises the following steps: extracting a percolate, adsorbing the percolate through macroporous resin, eluting with water and ethanol in sequence, combining an obtained lower column liquid and a water washing liquid, separating through a ceramic membrane and an ultrafiltration membrane in sequence to obtain a micromolecular functional sugar part, decolorizing with activated carbon, eluting with an ethanol solution, concentrating and drying to obtain a polyphenol part, and proportioning to obtain a target product. According to the technical scheme provided by the invention, active micromolecular functional sugar and polyphenol components in the phyllanthus emblica can be extracted and enriched at one time. The extract is light in color and good in solubility, so that the problems of single extracted component and inconvenience in application due to poor color and solubility in the traditional process are effectively solved.

The method comprises the following steps: A, mixing the lime precipitate with hot water, uniformly stirring and dispersing, adding a strong alkali solution, controlling the pH value to be 10-12, and reacting for 1-4 hours while stirring to obtain a mixed solution A; b, cooling the mixed solution A, and filtering to obtain filtrate and filter residues; taking the filtrate to obtain a gallium-containing pregnant solution; and C, the gallium-containing pregnant solution is sent back to a macroporous resin adsorption procedure for recycling gallium from the mother solution, and gallium recycling is carried out. According to the method, gallium in the gallium-containing lime sediment can be effectively recycled, the problem that the gallium content in the gallium-containing lime sediment is high is solved, and the gallium recycling rate is increased.

The invention discloses a cassia twig composition, a preparation method thereof and application of the cassia twig composition in preparation of medicine for treating gynecological diseases, and relates to the technical field of medicines.The preparation method comprises the following steps that 1, cassia twigs and licorice roots are mixed, alcohol is added, ultrasonic treatment is conducted, and an alcohol extract and medicine residues are obtained; (2) mixing the medicine residue, radix paeoniae alba, jujube and ginger, and performing water extraction to obtain a water extract; (3) concentrating the alcohol extract, and treating the concentrated alcohol extract with macroporous resin to obtain an eluent; and (4) combining the eluent and the water extract, concentrating, and drying to obtain the product. The macroporous resin treatment comprises the following steps: loading a sample at the flow rate of 2-8 BV / h by the volume of a loading liquid of 5-10 BV, and eluting with 70-80% ethanol of 3-7 BV at the flow rate of 1-5 BV / h. Compared with the prior art, the cassia twig composition prepared by the invention can better treat dysmenorrhea caused by congealing cold and blood stasis, and provides more choices for treatment of gynecological diseases such as primary dysmenorrhea and the like.

The invention discloses a paris polyphylla extract which is prepared by the following steps: extracting crushed paris polyphylla for 1-3 times by adopting a cyclodextrin solution at 30-70 DEG C under ultrasonic waves, collecting and combining an extracting solution, separating and purifying the extracting solution through macroporous resin, eluting the extracting solution through an ethanol solution with the volume concentration of 70%, and concentrating and drying the eluent, thereby obtaining the paris polyphylla extract. The paris polyphylla extract is applied to treatment of bladder cancer, experimental results show that the paris polyphylla extract has an obvious inhibiting effect on proliferation of human bladder cancer cells T24 and UM-UC-3, a theoretical basis is provided for deep research on the anti-tumor action mechanism of paris polyphylla, and the paris polyphylla extract is of great significance in revealing research on anti-tumor effective active ingredients of paris polyphylla and has a wide application prospect. The invention provides a new way and means for treating tumors.

The utility model discloses a solvent recovery device based on macroporous resin adsorption, relates to the technical field of resin solvent recovery, and adopts the technical scheme that a flattening mechanism comprises a plurality of groups of motors, one side of each group of motor is mounted on one side of a shell, and the other side of each group of motor is mounted on the other side of the shell; output shafts of the multiple sets of motors penetrate through one side of the shell and are connected with one end of a screw rod, the other end of the screw rod is installed in the shell through a bearing, the outer surface of the screw rod is in threaded connection with a threaded seat, and the lower portion of a transverse plate is connected with a scraping plate through an adjusting mechanism. The scraper is driven to move, macroporous resin placed above the filter plate is flattened, the contact area of the macroporous resin and high-temperature steam is increased, the macroporous resin can fully react with the high-temperature steam, and therefore the problem that the extraction rate of a solvent is reduced due to the fact that part of the macroporous resin and the high-temperature steam are not fully reacted is solved.

The invention relates to a method for extracting and purifying lycium barbarum polyphenol by combining bacteria and enzymes. The method comprises the following steps: pretreating lycium barbarum fruits to obtain lycium barbarum powder; extracting the wolfberry powder by using a first solvent to obtain a first crude extract; fermenting the first crude extract with lactobacillus plantarum to obtain a second crude extract; performing enzymolysis on the second crude extract by using pectinase, hemicellulase and beta-glucosidase in sequence to obtain an enzymolysis extracting solution; adsorbing and purifying the enzymolysis extracting solution by using first ion exchange resin, washing and eluting to obtain a first eluent; adsorbing and purifying the first eluent by using second ion exchange resin, washing and eluting to obtain a second eluent; and concentrating and drying the second eluent to obtain wolfberry polyphenol powder. According to the method disclosed by the invention, the fermentation of lactobacillus plantarum is combined with the synergistic effect of pectinase, hemicellulase and beta-glucosidase, polysaccharide in a crude extract is decomposed, polyphenol is released and combined, the extraction rate and the purity of the polyphenol are improved, and meanwhile, a continuous purification process of macroporous resins AB-8 and D-101 is combined, so that the product quality is further improved.

The invention discloses radix notoginseng stem and leaf rare ginsenoside as well as a preparation method and application thereof, and belongs to the technical field of ginsenoside. The radix notoginseng stem and leaf rare ginsenoside comprises Rk3, Rh4, S-Rg3, R-Rg3, Rk1 and Rg5. The radix notoginseng stem and leaf rare ginsenoside can be used for preparing radix notoginseng stem and leaf rare ginsenoside. The preparation method comprises the following steps: mixing the pseudo-ginseng stem and leaf extract with an acetic acid aqueous solution, reacting at 105-120 DEG C for 10-40 minutes, and drying; in the preparation process, macroporous resin purification is not carried out. According to the scheme, part of chemical substances in the panax notoginseng stem and leaf extract can be rapidly converted at a low temperature, new active substances Rh4, S-Rg3, R-Rg3, Rk1 and Rg5 with high purity are obtained at the same time, and the obtained panax notoginseng stem and leaf rare ginsenoside can effectively inhibit propionibacterium acnes, burkholderia cepacia and malassezia furfur.

The invention relates to a method for efficiently extracting high-specification battery-grade lithium carbonate, and belongs to the technical field of lithium carbonate preparation. The method comprises the following steps: pretreatment: mixing a lithium-containing solution raw material with liquid caustic soda; extraction: fully mixing the lithium-containing solution with a no-load extraction agent, and performing phase splitting; reverse extraction: conveying sulfuric acid with a certain concentration and a lithium-containing load oil phase through a centrifugal pump respectively, fully mixing in an extraction tank, and splitting phases; calcium and magnesium are removed from the strip liquor, specifically, the strip liquor is adsorbed through resin, calcium and magnesium in the strip liquor are adsorbed and removed, and the resin is recycled through acid-base regeneration and water washing after adsorption saturation; wastewater treatment: firstly recycling an organic extracting agent in a non-dissolved state from the water-phase raffinate in the extraction procedure through an oil separator, and then treating and regenerating through a macroporous resin adsorption tank; precipitating lithium: placing the prepared sodium carbonate solution in a beaker, stirring and heating, slowly dropwise adding the lithium sulfate solution, continuously reacting, and filtering; and washing and drying: filtering, and drying the lithium carbonate obtained after filtering.

The invention discloses an egg yolk protein osteogenic peptide and a preparation method thereof, and belongs to the field of biological medicine and the technical field of functional food or egg product processing. According to the method, the skimmed yolk powder is subjected to enzymolysis to prepare the osteogenic bioactive peptide, enrichment and separation are carried out through macroporous resin, the preparation and enrichment process is suitable for large-scale production, and laboratory achievements can be promoted to be converted into industrial production; two osteogenic active peptides are separated and identified, DFDLPT has the activity of promoting osteoblast proliferation, and FDIDPG has the functional activity of promoting osteoblast differentiation and mineralization.

The invention relates to the technical field of plant polysaccharides, and particularly discloses a red-knees herb polysaccharide and a preparation method and application thereof, the peak top molecular weight of the red-knees herb polysaccharide is 33.9 KDa, the weight-average molecular weight of the red-knees herb polysaccharide is 42.8 KDa, the number-average molecular weight of the red-knees herb polysaccharide is 10.0 KDa, and the red-knees herb polysaccharide is mainly composed of galacturonic acid, galactose, arabinose, rhamnose and glucuronic acid. According to the preparation method of the red-knees herb polysaccharide, by optimizing process parameters and controlling the reasonable solid-liquid ratio, heating temperature and heating time, the dissolution rate of the polysaccharide is increased, the dissolution rate of impurities is reduced, and the yield of the red-knees herb polysaccharide is increased. The red-knees herb polysaccharide is used as a raw material, ethanol is used for alcohol precipitation, polysaccharide components are precipitated, impurities are preliminarily removed, then macroporous resin is used for purification, the recovery rate of polysaccharide and the removal rate of impurities such as pigment and protein are improved by screening optimization and pretreatment of the macroporous resin, and the total sugar content of the purified red-knees herb polysaccharide is 80% or above. Use of toxic chemical reagents is avoided, and the preparation method is safe and efficient.

The invention provides a method for extracting tobacco flavor from waste tobacco leaves, a flavor additive, an aerosol generating substrate and an aerosol generating product. The method for extracting the tobacco flavor from the waste tobacco leaves comprises the following steps: adding the waste tobacco leaves into dry distillation equipment, introducing water vapor and inert gas into the dry distillation equipment, heating the dry distillation equipment to 300-380 DEG C so as to simultaneously perform distillation and dry distillation on the waste tobacco leaves, collecting distillate in the whole distillation-dry distillation process, and adsorbing the distillate by using macroporous resin XAD-4, finally, ethyl alcohol with the volume fraction being 85%-95% is used for recycling the adsorbate, the tobacco perfume suitable for perfuming novel tobacco products is obtained, and meanwhile the perfume can be directly used as a perfume additive to be added into an aerosol generating substrate or an aerosol generating product. According to the method, efficient utilization of the waste tobacco leaves is achieved, and bad flavor substances are reduced through the method for extracting the tobacco flavor.

The invention relates to the technical field of extraction and processing of radix puerariae and perilla frutescens, and particularly discloses a compound extract of radix puerariae and perilla frutescens as well as a preparation method and application of the compound extract. The preparation method comprises the following steps: slicing radix puerariae, cleaning with purple perilla, drying, adding water to adjust the water content, adding a leavening agent, and fermenting to a specific pH value to obtain a fermented product; and performing reflux extraction on the fermentation product by using a deep-eutectic solvent to obtain the deep-eutectic solvent extract. Mixing the extract with ethanol, and performing supercritical carbon dioxide extraction to obtain a supercritical carbon dioxide extract. Carrying out water extraction and alcohol precipitation treatment on the extract to obtain a primarily purified extract; and finally, separating by using macroporous resin to obtain the final compound extract of the radix puerariae and the perilla frutescens. According to the method, the extraction rate of flavonoid compounds and other bioactive substances in the compound extract is remarkably increased, so that the compound extract shows excellent effects in the aspects of reducing blood pressure, reducing blood sugar, reducing blood fat and the like, can be used as a daily diet supplement, and is widely applied to the fields of foods, medicines and health-care products.

The invention provides a blood purification adsorbent and a preparation method thereof, the blood purification adsorbent takes macroporous resin containing an epoxy group as a carrier, the epoxy group on the carrier reacts with polyethyleneimine, the polyethyleneimine is immobilized on the carrier, the polyethyleneimine is taken as a spacer arm, and the epoxy group on the carrier reacts with the polyethyleneimine to prepare the blood purification adsorbent. Superoxide dismutase and lipoic acid are connected to the spacer arm through a dehydration condensation reaction, so that the superoxide dismutase and the lipoic acid are immobilized on the carrier. The blood purification adsorbent can remove superoxide, free radicals and inflammatory factors at the same time, and can effectively improve oxidative stress and microinflammation states.

The invention belongs to the field of plant extract processing, and particularly relates to a preparation method and application of a ceramide-containing platycodon grandiflorum extract. The preparation method comprises the following steps: adding crushed radix platycodonis rhizomes and leaves into 45% ethanol for soaking, ultrasonically treating a material-liquid mixture for 40 minutes, and taking residual radix platycodonis dregs as a raw material for preparing ceramide. The preparation method comprises the following steps: carrying out solid fermentation on a platycodon grandiflorum herb residue mixed strain S.paucimobilis for 72 hours, then extracting to obtain a ceramide crude product, and purifying through macroporous resin to obtain the ceramide-containing platycodon grandiflorum extract. The platycodon grandiflorum extract obtained by the method contains natural ceramide from a plant source, has no irritation to chick embryos, can be used as a cosmetic raw material, has soothing and repairing effects, and is beneficial to increasing the additional economic value of platycodon grandiflorum and promoting ecological environment protection.

The invention discloses gleditsia sinensis saponin with anti-glycosylation activity and a preparation method of the gleditsia sinensis saponin. The method comprises the following steps: washing, drying, crushing, degreasing and carrying out other pretreatment on gleditsia sinensis shells to obtain gleditsia sinensis powder; uniformly mixing gleditsia sinensis powder with a deep-eutectic solvent, extracting in an ultrasonic field, and centrifuging to obtain an extracting solution; dialyzing, concentrating and freeze-drying the extracting solution to obtain a gleditsia sinensis saponin crude extract, re-dissolving the crude extract in distilled water, and purifying by using macroporous resin and reversed-phase column chromatography in sequence to obtain purified gleditsia sinensis saponin; the method for preparing the gleditsia sinensis saponin is simple, convenient, rapid, green and environment-friendly, industrial production is facilitated, and the obtained saponin is high in purity and good in quality. In addition, the gleditsia sinensis saponin prepared by the method has the activity of inhibiting the formation of advanced glycosylation end products.

The invention discloses a radix asparagi extract and an application thereof in tumor resistance, and belongs to the field of biological medicine, radix asparagi is heated and extracted with ethanol with the concentration of 60% to obtain an alcohol extract, then the alcohol extract is added into macroporous resin, then elution is performed with ethanol with the concentration of 30% and ethanol with the concentration of 60% in sequence, ethanol eluate with the concentration of 60% is collected, and concentration and drying are performed to obtain the radix asparagi extract. The radix asparagi extract disclosed by the invention has a relatively good inhibition effect on various cancer cells.

The invention discloses a method for extracting ecdysterone from cyanotis arachnoidea by using a deep-eutectic solvent, and belongs to the technical field of ecdysterone extraction. The method comprises the following steps: mixing a deep-eutectic solvent which takes choline chloride and a hydrogen bond donor material as raw materials with water, extracting ecdysterone in roots and stems of Cyanotis arachnoidea to obtain an ecdysterone crude extract, precipitating the ecdysterone crude extract by adding water, filtering, and adsorbing ecdysterone by using macroporous resin. Adding water for elution to obtain a deep-eutectic solvent-water mixed solution, and recovering under reduced pressure to obtain an extraction solvent for cyclic utilization; and eluting by using alcohol with different concentrations to obtain an ecdysterone crude extract, and purifying the crude extract to obtain high-purity ecdysterone. Compared with a conventional extraction method, the process method adopted by the invention has the advantages that the process is simple, efficient, green and environment-friendly, the used eutectic solvent can be recycled, the loss caused by heating volatilization in the extraction process is avoided, the ecdysterone extracted by the method is pure white, the content of ecdysterone can reach 90% or above after simple purification, and the method is suitable for industrial production. After re-purification, the purity of ecdysterone detected by high performance liquid chromatography is up to 98% or above.

The present invention discloses a preparation method of a Rosa roxburghii Tratt extract rich in vitamin C, comprising the following steps: (1) washing fresh Rosa roxburghii Tratt fruits, and juicing them respectively after juicing or freezing; (2) taking the Rosa roxburghii Tratt juice, diluting it and passing it through an ultrafiltration membrane to obtain a filtrate; (3) taking the filtrate and concentrating it through a reverse osmosis membrane to obtain a concentrated Rosa roxburghii Tratt juice; (4) taking the concentrated Rosa roxburghii Tratt juice, adding ethanol, centrifuging to obtain a supernatant, and then concentrating and recovering the ethanol to obtain an alcohol-precipitated concentrated solution; (5) eluting the alcohol-precipitated concentrated solution on a macroporous resin, collecting the eluate and concentrating it to obtain an extract paste; (6) drying the extract paste to obtain a Rosa roxburghii Tratt extract rich in vitamin C, and also discloses the application of the extract. The preparation method of the present invention can make full use of fresh Rosa roxburghii Tratt fruits. Through the combination of various process steps, vitamin C is enriched. It is a natural vitamin C extract, and the antioxidant and whitening functions of the extract are significantly enhanced, providing a theoretical basis and application basis for promoting the development of the Rosa roxburghii Tratt industrial chain.

The invention relates to the technical field of polysaccharide preparation, in particular to mesona chinensis acidic polysaccharide and an extraction method thereof. The extraction method comprises the following steps: 1) crushing, degreasing and drying dried mesona chinensis, boiling and performing alkali extraction to obtain a mesona chinensis extracting solution; macroporous resin D-900 is adopted for adsorption to remove pigments, and crude mesona chinensis benth polysaccharide is obtained; carrying out primary purification by adopting DEAE-52 ion exchange column chromatography; and performing chromatographic purification through a Sephadex G-100 ion exchange column, collecting components, and performing freeze-drying to obtain the mesona chinensis benth acidic polysaccharide. The extraction method of the mesona chinensis benth acidic polysaccharide has the beneficial effects that the operation is simple, the cost is low, the characteristics of good decoloring effect and high polysaccharide preservation rate are also realized, and the biological activity of the mesona chinensis benth acidic polysaccharide can be guaranteed. Compared with other resins, the D-900 macroporous resin is used for selectively adsorbing melanin, the adsorption capacity of the D-900 macroporous resin to mesona melanin is remarkably higher than that of other resins, the adsorption rate of the D-900 macroporous resin to polysaccharide is lower than 2%, and the D-900 macroporous resin has the advantages of being good in decolorization effect and high in polysaccharide preservation rate.