545 results about "Macroporous resin" patented technology

The invention discloses a plant-source nutritional supplement based on glycoside compounds and a preparation method thereof, and relates to the technical field of preparation of plant-source nutritional supplements.The preparation method comprises the steps that raw materials are prepared; the preparation method comprises the following steps: respectively carrying out ultrasonic-assisted extraction on flavonoid glycoside, saponin and geniposide raw materials to obtain an extracting solution; carrying out centrifugal separation on the extracting solution, taking supernate, and then purifying by adopting a macroporous resin adsorption method to obtain a purified glycoside compound solution; mixing the purified glycoside compound solution with lycium barbarum polysaccharide, Chinese yam starch, vitamin E and other auxiliaries in proportion, and uniformly stirring to form a mixed solution; carrying out spray drying on the mixed solution to obtain a powdery nutritional supplement; and screening the powdery nutritional supplement. The high-purity plant source glycoside compound and the functional auxiliary agent are scientifically compounded, and ultrasonic extraction, resin purification and precise process control are combined, so that the integrated improvement of activity synergy, quality stability and personalized scene adaptation of the nutritional supplement is realized.

The invention discloses a method for extracting and purifying flavone in litsea coreana through an ultrasonic-assisted eutectic solvent, and belongs to the technical field of green processing of natural products. The invention provides a method for efficiently preparing litsea coreana flavone by integrating computer prediction, green solvent extraction and resin purification technologies. The method comprises the following steps: firstly, calculating and screening a deep eutectic solvent system with the highest affinity with the litsea coreana flavone on the basis of a COSMO-RS theoretical model, then coupling the optimized DES with an ultrasonic-assisted extraction (UAE) technology, and through the synergistic effect of the DES and the UAE technology, remarkably improving the extraction rate of the litsea coreana flavone and furthest keeping the biological activity of a target component; then, a macroporous resin dynamic adsorption-desorption purification process is linked, so that high-selectivity enrichment and impurity removal of the flavonoid compounds in the crude extract are realized, a high-purity product is obtained, and a complete process route from intelligent screening, efficient extraction to precise purification is finally formed.

The invention provides an adsorbent capable of simultaneously adsorbing bilirubin and cytokines and a preparation method and application thereof, and the preparation method comprises the following steps: carrying out suspension polymerization reaction on a styrene monomer and a polyvinyl cross-linking agent in a dispersion medium under the action of a continuous multi-component pore-foaming agent and an initiator to prepare polystyrene-based macroporous resin; carrying out chloromethylation reaction on the polystyrene-based macroporous resin to prepare chlorine balls; carrying out post-crosslinking reaction on the chloromethylated beads to prepare the adsorbent capable of adsorbing bilirubin and cytokines at the same time; the continuous multi-component pore-foaming agent comprises a first pore-foaming agent, a second pore-foaming agent and a third pore-foaming agent of which the solubility parameters are gradually increased in sequence; the temperature of the suspension polymerization reaction is adjusted according to the mass fraction of the polyvinyl cross-linking agent in the monomer mixture and the solubility parameter of the continuous multi-component pore-foaming agent, and the monomer mixture is composed of styrene monomers and the polyvinyl cross-linking agent. The bilirubin and cell factors can be adsorbed at the same time, and the safety of blood purification is improved.

The invention provides a percolation device and a preparation method of a phyllanthus emblica extract. The percolation device comprises a percolation tank, a pressing device and a plurality of separation structures, the preparation method of the emblic leafflower fruit extract comprises the following steps: firstly, coarsely crushing and soaking an emblic leafflower fruit medicinal material, then percolating the emblic leafflower fruit medicinal material in a percolating device, and collecting percolate; the preparation method comprises the following steps: extracting a percolate, adsorbing the percolate through macroporous resin, eluting with water and ethanol in sequence, combining an obtained lower column liquid and a water washing liquid, separating through a ceramic membrane and an ultrafiltration membrane in sequence to obtain a micromolecular functional sugar part, decolorizing with activated carbon, eluting with an ethanol solution, concentrating and drying to obtain a polyphenol part, and proportioning to obtain a target product. According to the technical scheme provided by the invention, active micromolecular functional sugar and polyphenol components in the phyllanthus emblica can be extracted and enriched at one time. The extract is light in color and good in solubility, so that the problems of single extracted component and inconvenience in application due to poor color and solubility in the traditional process are effectively solved.

The invention discloses a cassia twig composition, a preparation method thereof and application of the cassia twig composition in preparation of medicine for treating gynecological diseases, and relates to the technical field of medicines.The preparation method comprises the following steps that 1, cassia twigs and licorice roots are mixed, alcohol is added, ultrasonic treatment is conducted, and an alcohol extract and medicine residues are obtained; (2) mixing the medicine residue, radix paeoniae alba, jujube and ginger, and performing water extraction to obtain a water extract; (3) concentrating the alcohol extract, and treating the concentrated alcohol extract with macroporous resin to obtain an eluent; and (4) combining the eluent and the water extract, concentrating, and drying to obtain the product. The macroporous resin treatment comprises the following steps: loading a sample at the flow rate of 2-8 BV / h by the volume of a loading liquid of 5-10 BV, and eluting with 70-80% ethanol of 3-7 BV at the flow rate of 1-5 BV / h. Compared with the prior art, the cassia twig composition prepared by the invention can better treat dysmenorrhea caused by congealing cold and blood stasis, and provides more choices for treatment of gynecological diseases such as primary dysmenorrhea and the like.

The invention relates to a method for extracting and purifying lycium barbarum polyphenol by combining bacteria and enzymes. The method comprises the following steps: pretreating lycium barbarum fruits to obtain lycium barbarum powder; extracting the wolfberry powder by using a first solvent to obtain a first crude extract; fermenting the first crude extract with lactobacillus plantarum to obtain a second crude extract; performing enzymolysis on the second crude extract by using pectinase, hemicellulase and beta-glucosidase in sequence to obtain an enzymolysis extracting solution; adsorbing and purifying the enzymolysis extracting solution by using first ion exchange resin, washing and eluting to obtain a first eluent; adsorbing and purifying the first eluent by using second ion exchange resin, washing and eluting to obtain a second eluent; and concentrating and drying the second eluent to obtain wolfberry polyphenol powder. According to the method disclosed by the invention, the fermentation of lactobacillus plantarum is combined with the synergistic effect of pectinase, hemicellulase and beta-glucosidase, polysaccharide in a crude extract is decomposed, polyphenol is released and combined, the extraction rate and the purity of the polyphenol are improved, and meanwhile, a continuous purification process of macroporous resins AB-8 and D-101 is combined, so that the product quality is further improved.

The invention discloses radix notoginseng stem and leaf rare ginsenoside as well as a preparation method and application thereof, and belongs to the technical field of ginsenoside. The radix notoginseng stem and leaf rare ginsenoside comprises Rk3, Rh4, S-Rg3, R-Rg3, Rk1 and Rg5. The radix notoginseng stem and leaf rare ginsenoside can be used for preparing radix notoginseng stem and leaf rare ginsenoside. The preparation method comprises the following steps: mixing the pseudo-ginseng stem and leaf extract with an acetic acid aqueous solution, reacting at 105-120 DEG C for 10-40 minutes, and drying; in the preparation process, macroporous resin purification is not carried out. According to the scheme, part of chemical substances in the panax notoginseng stem and leaf extract can be rapidly converted at a low temperature, new active substances Rh4, S-Rg3, R-Rg3, Rk1 and Rg5 with high purity are obtained at the same time, and the obtained panax notoginseng stem and leaf rare ginsenoside can effectively inhibit propionibacterium acnes, burkholderia cepacia and malassezia furfur.

The invention relates to the technical field of plant polysaccharides, and particularly discloses a red-knees herb polysaccharide and a preparation method and application thereof, the peak top molecular weight of the red-knees herb polysaccharide is 33.9 KDa, the weight-average molecular weight of the red-knees herb polysaccharide is 42.8 KDa, the number-average molecular weight of the red-knees herb polysaccharide is 10.0 KDa, and the red-knees herb polysaccharide is mainly composed of galacturonic acid, galactose, arabinose, rhamnose and glucuronic acid. According to the preparation method of the red-knees herb polysaccharide, by optimizing process parameters and controlling the reasonable solid-liquid ratio, heating temperature and heating time, the dissolution rate of the polysaccharide is increased, the dissolution rate of impurities is reduced, and the yield of the red-knees herb polysaccharide is increased. The red-knees herb polysaccharide is used as a raw material, ethanol is used for alcohol precipitation, polysaccharide components are precipitated, impurities are preliminarily removed, then macroporous resin is used for purification, the recovery rate of polysaccharide and the removal rate of impurities such as pigment and protein are improved by screening optimization and pretreatment of the macroporous resin, and the total sugar content of the purified red-knees herb polysaccharide is 80% or above. Use of toxic chemical reagents is avoided, and the preparation method is safe and efficient.

The invention discloses a method for extracting ecdysterone from cyanotis arachnoidea by using a deep-eutectic solvent, and belongs to the technical field of ecdysterone extraction. The method comprises the following steps: mixing a deep-eutectic solvent which takes choline chloride and a hydrogen bond donor material as raw materials with water, extracting ecdysterone in roots and stems of Cyanotis arachnoidea to obtain an ecdysterone crude extract, precipitating the ecdysterone crude extract by adding water, filtering, and adsorbing ecdysterone by using macroporous resin. Adding water for elution to obtain a deep-eutectic solvent-water mixed solution, and recovering under reduced pressure to obtain an extraction solvent for cyclic utilization; and eluting by using alcohol with different concentrations to obtain an ecdysterone crude extract, and purifying the crude extract to obtain high-purity ecdysterone. Compared with a conventional extraction method, the process method adopted by the invention has the advantages that the process is simple, efficient, green and environment-friendly, the used eutectic solvent can be recycled, the loss caused by heating volatilization in the extraction process is avoided, the ecdysterone extracted by the method is pure white, the content of ecdysterone can reach 90% or above after simple purification, and the method is suitable for industrial production. After re-purification, the purity of ecdysterone detected by high performance liquid chromatography is up to 98% or above.

The invention relates to the technical field of polysaccharide preparation, in particular to mesona chinensis acidic polysaccharide and an extraction method thereof. The extraction method comprises the following steps: 1) crushing, degreasing and drying dried mesona chinensis, boiling and performing alkali extraction to obtain a mesona chinensis extracting solution; macroporous resin D-900 is adopted for adsorption to remove pigments, and crude mesona chinensis benth polysaccharide is obtained; carrying out primary purification by adopting DEAE-52 ion exchange column chromatography; and performing chromatographic purification through a Sephadex G-100 ion exchange column, collecting components, and performing freeze-drying to obtain the mesona chinensis benth acidic polysaccharide. The extraction method of the mesona chinensis benth acidic polysaccharide has the beneficial effects that the operation is simple, the cost is low, the characteristics of good decoloring effect and high polysaccharide preservation rate are also realized, and the biological activity of the mesona chinensis benth acidic polysaccharide can be guaranteed. Compared with other resins, the D-900 macroporous resin is used for selectively adsorbing melanin, the adsorption capacity of the D-900 macroporous resin to mesona melanin is remarkably higher than that of other resins, the adsorption rate of the D-900 macroporous resin to polysaccharide is lower than 2%, and the D-900 macroporous resin has the advantages of being good in decolorization effect and high in polysaccharide preservation rate.

The invention belongs to the technical field of active substance extraction, and discloses a method for extracting boxthorn leaf total flavonoids through an ultrasonic-assisted deep-eutectic solvent and application of the boxthorn leaf total flavonoids. Heating and mixing the hydrogen bond acceptor and the hydrogen bond donor, stirring to be transparent, and adding water to reduce viscosity to obtain the eutectic solvent; mixing the boxthorn leaf powder with a deep-eutectic solvent, performing ultrasonic extraction and centrifugation, and collecting supernate; adsorbing by using macroporous resin, washing and desorbing to obtain an eluent; and performing vacuum freeze drying to constant weight. According to the method disclosed by the invention, the eutectic solvent with low melting point and strong solubility is selected, the flavonoid compounds in the boxthorn leaves are green and efficiently extracted, and the total flavonoid content of the boxthorn leaves obtained by taking the eutectic solvent as an extraction solvent is 123.94 + / -1.04 mg / g and is far higher than the total flavonoid content of the boxthorn leaves obtained by traditional ethanol extraction by 64.60 + / -0.87 mg / g; the method is good in safety, short in time consumption and high in extraction rate.

The present application provides a kind of Radix Polygoni Multiflori active ingredient separation and purification process, belong to plant extraction technical field.The present application separation and purification process includes: Radix Polygoni Multiflori ethanol ultrasonic extraction, activated carbon adsorption, HPD-100 macroporous resin purification, membrane filtration, ultrafiltration, reduced pressure concentration and crystallization step, can efficiently extract Radix Polygoni Multiflori extract with high 5 alpha-reductase inhibitory activity.After compounding with the extract of Euonymus alatus obtained by specific microbial fermentation, the anti-hair loss effect can be significantly enhanced.The in vitro experimental results show that the Radix Polygoni Multiflori extract and the extract of Euonymus alatus both have significant 5 alpha-reductase inhibitory activity.The present application can effectively promote hair growth and improve hair loss, and the effect is close to the positive control drug.The present application process is stable, high in purity, strong in activity, suitable for industrial production, and has good market application prospect.

The invention provides a preparation method of yak bone collagen peptide, a composition for promoting skeletal development and application of the composition, and relates to the technical field of health food. The preparation method comprises the following steps: carrying out ultrasonic pretreatment on yak bones, and degreasing through supercritical CO2 to obtain a degreased product; mixing the degreased product with a fishy smell removing agent to obtain a fishy smell removing product; the fishy smell removing agent is an aqueous solution of a plant extract; the plant extract is selected from at least one of amomum tsao-ko extract, turmeric extract, green tea extract or momordica grosvenori extract; the fishy smell removal product is subjected to an enzymatic hydrolysis reaction through an immobilized compound enzyme reactor, wherein the compound enzyme is prolyl endopeptidase, papain and ficin; obtaining a crude product; carrying out ultrafiltration by adopting a filter membrane with the molecular weight cut-off of 1kDa, and adsorbing through macroporous resin. The yak bone collagen peptide and the composition prepared by the invention can obviously improve the body length, promote the skeletal development and promote the secretion of growth hormone, and meet the growth requirements of children.

The invention discloses a method for extracting an anti-aging component camellia saponin A from camellia on the basis of a full-bionic targeted extraction technology and application of the anti-aging component camellia saponin A. Efficient enrichment and structure protection of the camellia saponin A are achieved by simulating the whole process of decomposition and absorption of the oral cavity, the stomach and the intestinal tract of a human body. Specifically, the camellia flowers or leaves are pretreated through supercritical extraction, lipid and gel impurities which are contained in the camellia flowers or leaves and are prone to forming gelatinoids are removed, and convenience is provided for subsequent purification; the problems that the camellia saponin A is low in yield and easy to degrade are solved by applying a full-bionic enzymolysis technology, and meanwhile, impurities such as lipid, protein, tannin and sugar can be degraded through full-bionic extraction, so that convenience is provided for subsequent crystallization; and purifying by adopting a macroporous resin adsorption technology, and finally purifying by crystallization. The camellia saponin A obtained by the invention can better achieve the effects of improving wrinkles, repairing skin, brightening skin color and delaying senescence through multi-way and multi-azimuth anti-wrinkle, repairing and whitening effects, and can be widely applied to various types of cosmetics.

The invention discloses a green method for preparing bakuchiol and application of bakuchiol in oxidation resistance. The invention provides a green method for preparing bakuchiol, aiming at the problems of low extraction rate, large loss of active ingredients, harm to health due to solvent residue, complicated extraction process and the like in the current bakuchiol extraction process, a deep eutectic solvent is used as an extraction medium, and a fructus psoraleae extract is extracted under the assistance of ultrasonic waves. The bakuchiol is further obtained through macroporous resin purification and separation, and the bakuchiol prepared by the method has remarkable antioxidant activity. According to the preparation method provided by the invention, the extraction content of the bakuchiol is remarkably increased, the problems existing in a traditional extraction process are solved, compared with a traditional organic solvent extract, the preparation method is simple and easy to operate, and the prepared bakuchiol is high in content, better in biological activity, green, safe, free of solvent residues and suitable for industrial production. The compound can be used for preparing more antioxidant drugs.

The application discloses a method for extracting ketone ester from ginkgo leaves, products and applications thereof, and relates to the technical field of plant extraction. The method comprises the following steps: uniformly mixing ginkgo leaf dry powder with lime water suspension, then performing hydrothermal extraction, then separating to obtain filtrate, and adjusting the pH value of the filtrate to neutral to obtain pH-neutral filtrate; using a macroporous resin to perform adsorption and elution treatment on the pH-neutral filtrate to obtain eluate, and performing drying treatment on the eluate to obtain ginkgo leaf ketone ester; the elution treatment uses ethanol aqueous solutions with increasing concentrations in sequence. The method provided by the application can effectively improve the extraction yield of ginkgo leaf ketone ester, and the antioxidant effect of ginkgo leaf ketone ester obtained by using the method is greatly improved.

The invention provides a traditional Chinese medicine composition for reducing blood sugar and blood pressure and a preparation method thereof, and belongs to the technical field of biological medicine, the composition comprises ivory white eggplant extract, parsley extract, banana zucchini rattan extract, coloured bean puffball extract, eucommia ulmoides glycoside and puerarin. The preparation method comprises the following steps: carrying out common enzymolysis on ivory white eggplant by using cellulase, pectinase and papain, and purifying by using an XAD-16 macroporous resin column and a Sephadex LH-20 gel column to obtain an ivory white eggplant extract; the method comprises the following steps: extracting parsley with an ethanol aqueous solution, and purifying with an AB-8 macroporous adsorption resin column to obtain a parsley extract; the preparation method comprises the following steps: extracting banana zucchini rattan by using an ethanol water solution, and purifying by using a D101 macroporous adsorption resin column to obtain a banana zucchini rattan extract; colored bean puffball is subjected to common enzymolysis with chitinase and ficin, extracted with a two-phase solvent and purified with an HPD-100 macroporous adsorption resin column, and the colored bean puffball extract is prepared. All the components are matched to realize synergy of reducing blood sugar and blood pressure.

This invention relates to the field of plant component extraction technology, specifically to a method for efficiently extracting and purifying berberine. The method includes: (1) selecting dried stems of *Radermachera sinensis*, crushing and sieving them to obtain *Radermachera sinensis* powder; (2) weighing the *Radermachera sinensis* powder and mixing it with a 20-30% (w / w) ammonium sulfate solution, then adding a compound enzyme preparation for enzymatic hydrolysis, adding isopropanol to obtain a mixture, extracting the mixture using an ultrasonic-microwave synergistic extraction method, centrifuging the extracted mixture for 10-13 min at 3500-4000 rpm, removing the isopropanol layer by distillation to obtain a crude extract; (3) dissolving the crude extract in water and loading it onto a polar macroporous resin for multiple elutions to obtain berberine. Compared with the prior art, this invention uses an enzyme preparation followed by an aqueous two-phase solvent combined with ultrasonic-microwave extraction technology to promote the maximum release of berberine from *Radermachera sinensis* powder, thereby increasing the yield. Finally, a gradient elution with macroporous resin is used to achieve efficient extraction and purification of berberine.

This invention relates to a novel substance with in vitro anti-inflammatory activity, its preparation method, and its application. The chemical name of maltol-3-1'-ethoxyhexane-1”,2”,3”,4”,5”,6”-hexaol-(4'-2”)-glucopyranoside is C. 21 H 34 O 13 Preparation method: 1. Extract *Trollius chinensis* with 70% ethanol (v / v); 2. Concentrate the extract until no alcohol odor remains and load it onto a D101 macroporous resin column; 3. Elute the fraction from the D101 macroporous resin with 30% ethanol, separate by silica gel column chromatography and gel column chromatography, and then obtain *Trollius chinensis* glycoside C using high-performance liquid chromatography. *Trollius chinensis* glycoside C is used to prepare anti-inflammatory drugs. The IC50 of *Trollius chinensis* glycoside C in this invention inhibits the release of pro-inflammatory factors TNF-α and IL-6 from LPS-stimulated mouse macrophages (RAW264.7). 50 The concentrations were 22.8 μmol / L and 31.0 μmol / L, respectively, indicating that it has good in vitro anti-inflammatory activity. This invention belongs to the field of research on effective components of natural drugs.

The invention relates to a method for rapidly preparing vernonia anthelmintica dealicin from vernonia anthelmintica dealicin, which comprises the following steps: carrying out ultrasonic extraction by using ethanol, enriching by using macroporous resin to obtain a vernonia anthelmintica dealicin extract, and carrying out extraction and pilot plant test preparative liquid chromatography separation to obtain a high-purity vernonia anthelmintica dealicin compound. The results of subsequent researches show that the vernonia anthelmintica dealicin can significantly inhibit the secretion of the chemotactic factor, and the inhibition rate of 1mol / L of vernonia anthelmintica dealicin on the release level of the chemotactic factor CXCL10 reaches 75.83%. The vernonia anthelmintica macrobrachin reduces the recruitment of self-reactive T cells to the skin by blocking a CXCL10-mediated inflammation signal channel, so that melanocytes are protected, and pigment regeneration is promoted. Therefore, the vernonia anthelmintica macrobrachin inhibits the secretion of the chemotactic factor CXCL10 in a targeted manner, can be used as a potential effective monomeric compound in the development of vitiligo medicines for research, and is expected to become a new strategy for treating vitiligo.

This invention relates to the field of natural product separation technology, specifically to coumarin compounds isolated from *Hypericum perforatum*, their preparation methods, and applications. The preparation method of these coumarin compounds includes alcohol extraction from *Hypericum perforatum*, followed by macroporous resin adsorption separation and column separation. The coumarin compounds of this invention can be used for anti-inflammatory purposes, exhibiting inhibitory activity against RAW264.7 cells and the expression of TNF-α, IL-1β, and IL-6 proteins.

The invention provides a separation and purification process of active ingredients of polygonum multiflorum, and belongs to the technical field of plant extraction. The separation and purification process comprises the steps of ethanol ultrasonic extraction of polygonum multiflorum, activated carbon adsorption, HPD-100 macroporous resin purification, membrane filtration, ultrafiltration, vacuum concentration and crystallization, and the polygonum multiflorum extract with high 5alpha-reductase inhibitory activity can be efficiently extracted. The anti-hair loss effect can be obviously enhanced after the compound is compounded with the eucommia ulmoides vine extract obtained through specific microbial fermentation. In-vitro experiment results show that both the polygonum multiflorum extract and the eucommia ulmoides vine extract have remarkable 5alpha-reductase inhibitory activity. The anti-hair loss composition can effectively promote hair growth and improve the hair loss condition, and the effect of the anti-hair loss composition is close to that of a positive control drug. The process is stable, the purity is high, the activity is strong, the method is suitable for industrial production, and the market application prospect is good.

The invention belongs to the technical field of biological medicine, and particularly relates to a sophora flower extract for inhibiting formation of an oral biofilm and application thereof. According to the method, the sophora flower extract obtained after alcohol extraction is subjected to specific macroporous resin column elution conditions, the sophora flower extract obtained through enrichment has a very excellent effect of inhibiting oral biofilm formation, and further elution is carried out under specific silica gel column conditions, so that the effect of the obtained sophora flower extract is further improved. The inhibition rate of the obtained sophora flower extract is obviously higher than that of sophora flower extracts prepared by traditional alcohol extraction or other processes, and the sophora flower extract has important application value in preparation of products for inhibiting and / or killing oral microorganisms or biological membranes thereof, and can be used for preventing and / or treating dental bacterial diseases including decayed teeth.

The present invention discloses a sesquiterpene glycoside compound and its preparation method and application. A new sesquiterpene glycoside ligulariatinside A is isolated and identified from the n-butanol extraction part of 95% ethanol extract from the root of Ligularia glossata by multiple chromatographic methods such as macroporous resin column chromatography, positive and reverse phase silica gel column chromatography and high performance liquid chromatography, as well as spectral methods. The molecular formula of the new sesquiterpene glycoside ligulariatinside A is determined to be C 21 H 34 O7, the structure is (3 S ,4 R ,5 R ,7 R )‑11‑hydroxy‑11,12‑dihydronootkatone‑11‑ O ‑ β ‑D‑glucopyranoside enriches the chemical diversity of Ligularia officinalis. Bacterial inhibition experiments revealed that the new sesquiterpenoid glycoside exhibits inhibitory activity against Vibrio anguillarum, with a minimum inhibitory concentration (MIC) of 50 μg / mL, laying the foundation for the development of new antibacterial drugs.

The application discloses a method for extracting soybean saponins and soybean oligosaccharides, and belongs to the technical field of natural product extraction. The method comprises the following steps: puffing treatment of soybean meal, wall breaking treatment in a high-voltage electric field; acid precipitation, collection of supernatant; after ultrasonic oscillation of the supernatant, a composite enzyme is added for enzymolysis, and a material liquid after enzymolysis is obtained; after filtration of the material liquid, the material liquid is loaded onto a macroporous resin column for adsorption, and after completion of the adsorption, effluent is collected; the effluent is purified and concentrated to obtain soybean oligosaccharides; an ethanol solution is used to elute the resin column, and eluate is collected; the eluate is recovered by ethanol and dried to obtain soybean saponins; the composite enzyme comprises amylase and beta-glucosidase. The method can significantly improve the extraction rate and purity of soybean saponins and soybean oligosaccharides, the whole process uses water as the main solvent, the consumption of organic solvents and the pollution to the environment are reduced, and the unit process water consumption, unit energy consumption and unit cost are reduced.

The invention relates to an uncaria alkaloid extract as well as a preparation method and application thereof. The method comprises the following steps: heating and leaching uncaria stem and branch powder with acidic ethanol to obtain an acid alcohol leaching solution; carrying out reduced pressure distillation and concentration on the leach liquor to obtain an uncaria total alkaloid extract; removing acid-insoluble substances from the total alkaloid extract, degreasing, adjusting alkali, extracting with chloroform, and recovering a chloroform layer to obtain uncaria crude alkaloid; and dissolving the uncaria crude alkaloid in distilled water, adding into a macroporous resin chromatographic column, adsorbing, flushing, eluting by using a 20% ethanol solution with five column volumes, and collecting the eluent to obtain the uncaria alkaloid extract. The ramulus uncariae cum uncis alkaloid extract PURA1 has the effects of improving the pathological phenotype of the Alzheimer's disease model caenorhabditis elegans and improving the oxidative stress resistance of the caenorhabditis elegans, plays a role in prolonging the service life of the caenorhabditis elegans by regulating and controlling the p38MAPK signal channel of the caenorhabditis elegans, and can be used for preparing the medicine for relieving the Alzheimer's disease and resisting aging.

The invention discloses a method for extracting characteristic components in Zhejiang radix tetrastigme, which comprises the following steps: (1) collecting a Zhejiang radix tetrastigme medicinal material, drying in the sun and crushing; (2) extracting, separating and purifying the medicinal materials by the following steps: a, extracting the medicinal materials with alcohol for many times, filtering, combining filtrates, and concentrating under reduced pressure until no alcohol exists; b, diluting the extracted concentrated solution with water, loading the diluted concentrated solution on macroporous resin, washing with water, eluting with 80% ethanol, and collecting the 80% ethanol water part; c, adopting a high-pressure preparation column to obtain target components LF01 and LF02; d, preparing by using a high-pressure preparation column, and freeze-drying to obtain a compound 1; and e, carrying out column separation to obtain a component with the HPLC purity of 92%, preparing by adopting a high-pressure preparative column, and carrying out freeze drying to obtain a compound 2. According to the method, the characteristic components of the Zhejiang radix tetrastigme medicinal material are extracted, separated and purified, and are subjected to structural identification, so that the problem of obtaining the characteristic components in the Zhejiang radix tetrastigme can be effectively solved.

This invention discloses a Jacaranda flower extract, its preparation method, and its applications, belonging to the field of pharmaceutical technology. The preparation method includes: drying and pulverizing Jacaranda flowers, extracting them by heating and reflux with an ethanol-water solution, concentrating the extract under reduced pressure to obtain a first crude extract; mixing the first crude extract with an aqueous two-phase mixture for extraction, concentrating the upper phase under reduced pressure to obtain a second crude extract; purifying and eluting the second crude extract using a macroporous resin, decolorizing the eluent, and drying to obtain the Jacaranda flower extract. The Jacaranda flower extract obtained by this method has clearly defined active ingredients, exhibits significant photoprotective effects against UVB, and demonstrates significant repair effects after UVB photodamage. It is highly safe and can be used as an active ingredient in skincare products for sun protection.

The utility model discloses a separation and purification device based on macroporous resin, and relates to the technical field of separation and purification, the separation and purification device based on macroporous resin comprises a chromatographic column, the chromatographic column is provided with a chromatographic structure convenient for separation and purification, the chromatographic structure comprises a feed port, a fixed base and a circular truncated cone flow guide ring, the feeding hole is fixedly formed in the upper end part of the chromatographic column, the fixed base is positioned at the lower end part of the chromatographic column, each circular truncated cone flow guide ring is positioned on the inner side wall of the chromatographic column, an adjusting valve is arranged at the lower end part of the chromatographic column, and two column mounting grooves are formed in the upper end part of the feeding hole. The chromatographic column is arranged to purify anthocyanin, the purity of the obtained anthocyanin is higher than that of unpurified anthocyanin, toxic organic solvents are avoided in the whole process, only ethyl alcohol is used, environment friendliness is achieved, resin can be repeatedly used, and the production cost is reduced.

The invention discloses a separation and purification method of total flavonoids of mesembryanthemum crystallinum, which comprises the following specific steps: firstly, washing, drying and crushing fresh mesembryanthemum crystallinum to obtain dry mesembryanthemum crystallinum powder; adding the dried mesembryanthemum crystallinum powder into an ethanol water solution, performing ultrasonic extraction to obtain a mesembryanthemum crystallinum total flavone extracting solution, and performing freeze-drying to obtain a mesembryanthemum crystallinum total flavone crude extract; and selecting HPD-722 type macroporous resin, and carrying out dynamic adsorption elution on the mesembryanthemum crystallinum total flavone crude extract to obtain a mesembryanthemum crystallinum total flavone refined product. The mesembryanthemum crystallinum total flavonoids with high purity are separated and purified from fresh mesembryanthemum crystallinum, the purity of the mesembryanthemum crystallinum total flavonoids before and after a dynamic separation and purification test is improved by more than three times, and the purification effect is ideal.