216 results about "Glycoside Compound" patented technology

The invention discloses a plant-source nutritional supplement based on glycoside compounds and a preparation method thereof, and relates to the technical field of preparation of plant-source nutritional supplements.The preparation method comprises the steps that raw materials are prepared; the preparation method comprises the following steps: respectively carrying out ultrasonic-assisted extraction on flavonoid glycoside, saponin and geniposide raw materials to obtain an extracting solution; carrying out centrifugal separation on the extracting solution, taking supernate, and then purifying by adopting a macroporous resin adsorption method to obtain a purified glycoside compound solution; mixing the purified glycoside compound solution with lycium barbarum polysaccharide, Chinese yam starch, vitamin E and other auxiliaries in proportion, and uniformly stirring to form a mixed solution; carrying out spray drying on the mixed solution to obtain a powdery nutritional supplement; and screening the powdery nutritional supplement. The high-purity plant source glycoside compound and the functional auxiliary agent are scientifically compounded, and ultrasonic extraction, resin purification and precise process control are combined, so that the integrated improvement of activity synergy, quality stability and personalized scene adaptation of the nutritional supplement is realized.

The invention discloses a preparation method and application of a novel glycoside component in eupatorium chinense. The compound is separated from an eupatorium chinense extract. The eupatorium chinense root extract is separated by using an extraction method, macroporous adsorption resin impurity removal, HW-40F gel column chromatography, high performance liquid chromatography and other methods to obtain a new glycoside compound. The inhibitory activity of the separated compound on diabetes related targets alpha-glucosidase and protein tyrosine phosphatase 1B (PTP1B) is tested, the experimental result shows that the compound has good inhibitory activity on two enzymes, and the molecular docking experiment also shows that the compound has good inhibitory activity on the two enzymes. The compound has good binding energy with two target protein alpha-glucosidase and protein tyrosine phosphatase 1B. Therefore, the compound can be used for preparing medicines for preventing, delaying or treating alpha-glucosidase or PTP1B-mediated diseases, especially type II diabetes and obesity, or can be used as a lead compound of the medicines.

The invention belongs to the field of biosynthesis, and particularly relates to a saccharomyces cerevisiae engineering bacterium for producing glycosyl donor UDP-arabinose as well as a construction method and application of the saccharomyces cerevisiae engineering bacterium. According to the invention, UDP-Glc dehydrogenase, UDP-GlcA decarboxylase, UDP-Xyl-4-epimerase or UDP-Glc-epimerase are sequentially expressed in WAT11, so that a synthetic route of UDP-Ara is introduced into the saccharomyces cerevisiae for the first time; de novo synthesis of Ara-oleanolic acid is realized by introducing a synthetic route of arabinosyl transferase and oleanolic acid; the yeast containing the UDP-Ara synthetic pathway and the arabinosyl transferase gene can also utilize betulinic acid to generate Ara-betulinic acid. The invention solves the problem of yeast total synthesis of the arabinosyl donor, provides a new biotechnology platform for production of natural glycoside compounds, and has important theoretical value and wide application prospect.

The invention discloses a coumarin tyramine glycoside compound and application thereof in preparation of PTP1B (protein tyrosine phosphatase 1B) inhibitors and antidiabetic drugs. The structural formula of the compound is # imgabs0 #, in the formula, R represents fat (aromatic) acyl or monosaccharide substituent, and R'represents hydrogen atom or acetyl; 4-methoxycoumarin 4 '-acetyl-O-L-rhamnose is used as an initial raw material, fat (aromatic) acyl or monosaccharide substituent groups are introduced into hydroxyl at the second position and hydroxyl at the third position of L-rhamnose at the same time, and then protecting groups of glycosyl are removed, so that the L-rhamnose is prepared. Pharmacological activity tests show that the compounds have good inhibitory activity on protein tyrosine phosphatase 1B, which indicates that the coumarin tyramine glycoside compounds can be researched and utilized as novel PTP1B inhibitors and antidiabetic drugs.

The invention discloses a silverweed cinquefoil root composition with an anti-hypoxia function and a preparation method thereof, and belongs to the technical field of medical compositions.The silverweed cinquefoil root composition comprises silverweed cinquefoil root polysaccharide, silverweed cinquefoil root polyphenol and silverweed cinquefoil root glycoside compounds according to the mass ratio of (0.6-0.8): (0.2-0.4): (0-0.05), and the silverweed cinquefoil root glycoside compounds comprise roxburgh rose glycoside and multiflora rose glycoside according to the mass ratio of (0.05-0.45): 1. The compounding of the potentilla anserine polysaccharide and the potentilla anserine polyphenol can provide a comprehensive free radical scavenging effect, maintain the dynamic balance of generation and scavenging of oxygen free radicals in an organism, coordinate cell energy metabolism and help the organism to better utilize limited oxygen resources. The silverweed cinquefoil root glycoside compound is used as an additional antioxidant, oxidative stress reaction and side reaction can be reduced, and the obtained silverweed cinquefoil root composition can well improve the anti-hypoxia capacity of an organism.

The invention discloses glycosyltransferase in safflower and a related biological material and application thereof. The invention firstly discloses any one of the following proteins: A1) a protein consisting of an amino acid sequence as shown in a sequence 2; a2) a fusion protein obtained by connecting the N end or / and C end of the protein as shown in the sequence 2 with a protein tag. The invention further discloses the protein related biological material and application thereof. The HSYA biosynthesis key glycosyl transferase gene UGT708U5 is obtained from safflower for the first time, it is proved that the protein UGT708U5 coded by the gene can catalyze phloretin to form HSYA precursor compounds, namely phloretin-mono-C-glucoside and phloretin-di-C-glucoside, and the gene plays an important role in biosynthesis and heterologous production of HSYA; the method has important theoretical and practical significance for regulating and producing the quinonechalcone C-glycoside compounds of plants and cultivating high-quality safflower carthamus.

The present invention discloses a method for extracting effective active ingredients from Buddleja buddhist flower and its application. The method comprises the following steps: first subjecting the crushed Buddleja buddhist flower to ethanol reflux extraction and concentration, and then extracting the obtained Buddleja buddhist flower alcohol extract with petroleum ether, ethyl acetate, and n-butanol respectively. The remaining aqueous phase is concentrated under reduced pressure, purified by a D101 macroporous resin column, and the target product obtained after drying is structurally identified as a verbascoside monomer compound. The method of the present invention can better control the quality standards of Buddleja buddhist flower and pave the way for a more in-depth study of its pharmacological effects by effectively separating the verbascoside compounds from the Buddleja buddhist flower, thereby laying the foundation for the clinical development of new drugs with anti-tumor, antioxidant, and immunomodulatory activities.

The invention relates to the technical field of chemistry and medicine, in particular to application of two phenylethanoid glycoside compounds in Pensamie in anti-hepatitis B virus and liver protection, including application of forsythiaside F and forsythiaside B in preparation of anti-hepatitis B virus drugs and / or application of forsythiaside F and forsythiaside B in preparation of liver protection drugs. According to the invention, two compounds of forsythiaside F and forsythiaside B are obtained from 75% ethanol extract of separated and purified Tibesimie, the properties of the two compounds are researched, and the two compounds are found to be capable of reducing HBsAg and HBeAg and have a certain liver protection effect, so that data are provided for development and utilization of Tibesimie and research and development of anti-hepatitis B virus drugs, and the research and development of the Tibesimie and the research and development of the anti-hepatitis B virus drugs are facilitated. And a new way is provided for HBV prevention and treatment.

The invention discloses application of glycoside compounds in prevention and treatment of plant pathogenic viruses, and belongs to the technical field of pesticide antivirus. According to the invention, targeting tobacco mosaic virus capsid protein is taken as an example, and small molecule picroside I (A0) and ZINC85593196 (B0) containing sugar rings are screened through virtual screening, molecular dynamics simulation, molecular mechanics and MM / GBSA free energy calculation. On the basis of further optimization, six compounds of 4-methylumbelliferone-beta-D-xyloside (A1), geniposide (A2), 4-methylumbelliferone acyl-beta-D-glucopyranoside (A3), 4-methylumbelliferone acyl-beta-D-glucopyranoside (A4), catalpol (A5) and aesculin (sesquihydrate) (A6) which are similar to the A0 in structure are obtained, and in-vivo and in-vitro biological activity evaluation is carried out. The active component screened by the method has a good inhibition effect on plant viruses.

The present invention discloses a sesquiterpene glycoside compound and its preparation method and application. A new sesquiterpene glycoside ligulariatinside A is isolated and identified from the n-butanol extraction part of 95% ethanol extract from the root of Ligularia glossata by multiple chromatographic methods such as macroporous resin column chromatography, positive and reverse phase silica gel column chromatography and high performance liquid chromatography, as well as spectral methods. The molecular formula of the new sesquiterpene glycoside ligulariatinside A is determined to be C 21 H 34 O7, the structure is (3 S ,4 R ,5 R ,7 R )‑11‑hydroxy‑11,12‑dihydronootkatone‑11‑ O ‑ β ‑D‑glucopyranoside enriches the chemical diversity of Ligularia officinalis. Bacterial inhibition experiments revealed that the new sesquiterpenoid glycoside exhibits inhibitory activity against Vibrio anguillarum, with a minimum inhibitory concentration (MIC) of 50 μg / mL, laying the foundation for the development of new antibacterial drugs.

This invention discloses a novel chalcone gypsum glycoside compound with the structure shown in Formula I. The chalcone gypsum glycoside compound of this invention has a novel and unique structure, and for the first time, it is disclosed that this compound possesses significant hepatoprotective activity and can be used in the preparation of hepatoprotective drugs. This invention also discloses the use of the aforementioned novel chalcone gypsum glycoside compound in the preparation of hepatoprotective drugs.

The invention belongs to the technical field of medicines, and discloses preparation of a glycoside compound and application of the glycoside compound in acute kidney injury, and the structural formula of the glycoside compound K30 is shown in the specification. The K30 compound is obtained by improving the chemical structure of the glycoside compound and the corresponding preparation method, and the K30 compound can be particularly used for treating acute kidney injury.

The invention discloses a synthesis method of chiral quaternary carbon glucoside, and belongs to the technical field of organic synthesis. The method disclosed by the invention comprises the following steps: a coupling reagent (aryl bromide, alkenyl bromide, alkynyl bromide, electron-deficient olefin and the like) and glucoside are fully reacted under the irradiation of purple light through catalysis of a photocatalyst or catalysis of the photocatalyst with one or more of a metal catalyst, a ligand and alkali, and the functionalized chiral quaternary carbon-containing glucoside is obtained. According to the invention, synthesis of the glucoside compound containing the chiral quaternary carbon center is realized by utilizing a light / metal concerted catalysis strategy. The method not only is mild in condition and wide in substrate adaptability, but also has the advantages of low cost, high atom utilization rate and economical steps due to the fact that simple and stable glucoside is directly used as a raw material. Through subsequent conversion, synthesis of an anti-cancer drug acetylcholine analogue can also be realized by containing chiral quaternary carbon glycoside, and the chiral quaternary carbon glycoside has a huge synthesis value.

The invention relates to a sesquiterpenoid glycoside compound in Atractylodes lancea, and a preparation method and application thereof. The invention belongs to the technical field of medicines, and relates to a method for extracting and separating a sesquiterpenoid glycoside compound (1S, 5S, 7R, 10S)-10-hydroxy-deoxy split-ring atractylodes macrocephala delta-lactone-11-O-beta-D-glucopyranoside from rhizome of Atractylodes lancea by using normal phase silica gel, reverse phase silica gel, preparative high performance liquid chromatography and other technologies. In-vitro anti-tumor activity screening experiments show that the compound has good anti-tumor activity, including obvious inhibition effects on human lung cancer cells A549, human cervical cancer cells Hela and human liver cancer cells HepG-2. Therefore, the sesquiterpenoid glycoside compound has a prospect of being developed into antitumor drugs.

The invention relates to the technical field of medicines, in particular to glycoside compounds as well as a preparation method and application thereof. The glycoside compound is selected from compounds represented by formulas (1) to (4) or medicinal salts represented by formulas (1) to (4). The glycoside compound is used for preparing the anti-inflammatory medicine. The siraitia grosvenorii extracting solution is systematically separated and purified by adopting multiple chromatographic technologies, and is subjected to structural identification, so that the inhibition effect of the separated compound on LPS-induced RAW264.7 cell NO is evaluated, and a foundation is laid for developing a novel anti-inflammatory drug and promoting high-value utilization of the resource.

The invention belongs to the field of natural medicines and chemical medicines for treating and preventing depression, and particularly discloses an iridoid glycoside compound, a preparation method thereof and application of the iridoid glycoside compound in preparation of antidepressants.The iridoid glycoside compound extracted from allamanoid C, allamanoid E and allamanoid H has a brand-new chemical structure, can be used for preparing antidepressants.The iridoid glycoside compound can be used for preparing antidepressants.The application of the iridoid glycoside compound to the antidepressants.The application of the iridoid glycoside compound to the antidepressants.The application of the iridoid glycoside compound to the antidepressants.The application of the iridoid glycoside compound to the antidepressants.The application of the the toxicity to normal cells is low; the allamanoid C, the allamanoid E and the allamanoid H have a remarkable neuroprotective effect on HT22 cell injury induced by corticosterone at a relatively low concentration, and the activity of the allamanoid C, the allamanoid E and the allamanoid H is higher than that of a positive control drug fluoxetine hydrochloride; the allamanoid C, the allamanoid E and the allamanoid H have an obvious anti-depression effect on despair depression models (a tail suspension model and a forced swimming model) of mice, so that the allamanoid C, the allamanoid E and the allamanoid H can be used for preparing the anti-depression medicine.

The invention provides a synthesis method of a quaternary cycloalkane modified O-glucoside compound shown in formula 3, in the presence of a catalyst, a sugar compound shown in formula 1 with exposed hydroxyl and a quaternary tension ring substrate compound shown in formula 2 are subjected to nucleophilic addition reaction in an organic solvent to prepare the quaternary cycloalkane modified O-glucoside compound shown in formula 3: X is H, CF3 or Cl; a is a dioxolane type compound or a pyran type compound; the catalyst is phosphazene base P4-t-Bu, and the specific structural formula is shown in the specification.

The invention provides a method for stereoselectively preparing a cis-2-boryl-alpha-carbon glycoside compound, and particularly provides a method for preparing a compound as shown in a formula 1 in the specification. The method comprises the step of reacting a compound shown in a formula 2, a compound shown in a formula 3 and a compound shown in a formula 4 under the action of a nickel salt catalyst, an amino alcohol ligand and alkali to obtain a compound shown in a formula 1. The method is simple to operate. # imgabs0 #

The invention discloses a benzofuran glycoside compound as well as a separation and extraction method, a pharmaceutical composition and application thereof. The compound 1 is separated from an eupatorium chinense extract. And separating and purifying chemical components of the n-butyl alcohol part of the eupatorium chinense root to obtain the benzofuran compound. The inhibition activity of target cyclooxygenase-2 of inflammatory related diseases of all the separated compounds 1 is tested, and the compounds 1 have good inhibition activity on COX-2 enzyme and have good binding energy with target protein. In addition, the compound also has a good NLRP3-related inhibition effect, can be independently used or combined with other medicines to regulate NLRP3-related proteins, and is used for treating NLRP3-related mediated diseases and / or diseases and preparing medicines for preventing or treating the diseases or diseases. The compound 1 also has a good inhibition effect on acetylcholin esterase, and can be used for treating Alzheimer's disease, myasthenia gravis, Parkinson's disease and other diseases.

The present invention belongs to the technical field of natural product extraction, and provides a flavone C-glycoside compound, an extraction method thereof and an application. The structural formula of the novel flavone C-glycoside compound is:. This compound has good inhibitory activity against EV71 virus, has no toxic effect on cells, has the potential to develop anti-EV71 virus drugs, and can be used as a raw material and a parent structure for new drug research and development.

The invention discloses application of an alkyl glycoside compound, belongs to the technical field of pesticides, particularly relates to application in insecticides, and provides a structural formula of the alkyl glycoside compound and an insecticide taking the alkyl glycoside compound as an effective component or an insecticide taking the alkyl glycoside compound and a common pesticide variety as effective components. The crop pest control effect of the pesticide preparation product is improved, and meanwhile the pesticide resistance of pests to the pesticide preparation product is slowed down.

The invention relates to the technical field of medicines, in particular to a novel triterpenoid glycoside compound extracted from rosa roxburghii tratt fruits and a preparation method and application of the novel triterpenoid glycoside compound. The novel triterpenoid glycoside compound is Roxbuterene E, the molecular formula of the novel triterpenoid glycoside compound is C41H66O13, the novel triterpenoid glycoside compound is obtained by separating and purifying a rosa roxburghii tratt fruit methanol extract by adopting normal-phase silica gel column chromatography, reversed-phase ODS column chromatography and HPLC (High Performance Liquid Chromatography), and a PTP1B enzyme inhibitory activity experiment proves that the novel triterpenoid glycoside compound has potential activity for treating diabetes. And technical support is provided for research and development of novel diabetes treatment drugs.

The invention discloses an application of a composition of isoliquiritigenin (glycoside), licochalcone and ethnic groups thereof in personal care products, and relates to the technical field of daily chemicals, and the composition is an anti-allergy soothing composition prepared based on a natural plant licorice extract isoliquiritigenin or a glycoside compound isoliquiritigenin thereof, licochalcone A and ethnic groups of licochalcone B, C, D, E and G. The traditional Chinese medicine composition has multiple functions of resisting allergy, relieving, resisting bacteria, diminishing inflammation, improving skin itch and stabbing pain and the like, does not contain hormones and antibiotics, has no toxic or side effect, is mild in effect and non-irritant to skin, can be used for treating skin allergy, red and swollen skin, itch and stabbing pain caused by various reasons, is applied to cosmetics and skin care products, such as gel, cream, masks and facial cleansers, and can be used for treating skin allergy, red and swollen skin, pruritus and stabbing pain caused by various reasons. The skin care product is mild to human bodies, safe and non-irritant, is suitable for sensitive skin, is suitable for people of all ages to use, has strong market competitiveness, and has relatively high economic and social benefits and the like.

The present invention discloses a method for preparing dihydroflavonoid glycoside compounds by industrial chromatography technology, the method comprising preparing a total flavonoid glycoside extract sample solution, adopting dynamic axial compression industrial chromatography purification, and detecting the separation condition by a dual-wavelength ultraviolet online detector; determining the start and end time of collecting the eluent of the dihydroflavonoid glycoside compounds according to the peak time and the height of the chromatographic peak, and concentrating the eluent under reduced pressure and vacuum drying. The purity of the prepared dihydroflavonoid glycoside compounds is above 98.5%; the separation process can be detected in real time online, the process has good reproducibility, simple operation, short preparation time, less organic solvent usage, economic and environmental protection, and can be mass-produced industrially.

The invention belongs to the technical field of synthesis of alkyl nitrogen glycoside compounds, and discloses an alkyl N-glycoside compound and a synthesis method thereof. The synthesis method comprises the following steps: uniformly dispersing manganese powder, a nickel catalyst and an organic ligand as shown in a formula 1 in a reaction solvent in an inert atmosphere, and uniformly mixing to obtain a reaction medium solution; a compound 1 as shown in a formula 1 and a compound 2 as shown in a formula 2 are sequentially dispersed in a reaction medium solution, stirred at room temperature and purified, and the alkyl N-glycoside compound as shown in a formula 3 is obtained. The synthesis method is simple and easy to operate, the synthesis condition is mild, the synthesis method can be suitable for different substrates, modular synthesis of the alkyl N-glycoside compound can be achieved, and the synthesis method is suitable for industrial production. The technical defects that in the prior art, reaction conditions of high temperature, strong base and the like are harsh, substrate synthesis is complex, the range is limited, and expensive photocatalysts are used are effectively overcome. # imgabs0 #

The inventive technology described herein includes systems, methods, and compositions for the upregulation of Uridine diphosphate glucose (UDPG) recycling in a yeast cell, by expressing a heterologous nucleotide sequence, operably linked to a promoter, encoding a phosphoglucomutase enzyme which catalyzes the conversion from glucose-6-phosphate to glucose- 1 -phosphate, and a UDP-glucose pyrophosphorylase enzyme which catalyzes the formation of UDPG from glucose 1 -phosphate and UTP. The increased levels of UDPG substrate produced by the yeast cell may be used as a substrate for a co-expressed UDP -glycosyltransferases enzyme capable of generating cannabinoid glycoside compounds.

The invention belongs to the technical field of desulfurizer preparation and flue gas purification treatment, and particularly relates to a calcium-based desulfurizer, a preparation method thereof and application of the calcium-based desulfurizer in high-concentration carbon dioxide flue gas. The preparation method of the calcium-based desulfurizing agent comprises the following steps: mixing quick lime, a first modifier, a second modifier and water, performing digestion reaction in a reactor, and drying the obtained reaction mixture to obtain the calcium-based desulfurizing agent, the first modifier is N-acyl amino acid salt formed by amino acid and derivatives thereof and C8-C18 alkyl chains through amido bonds; the second modifier is an alkyl glycoside compound. The key point of the invention is that two specific modifiers, namely N-acylamino acid salt and alkyl glycoside compounds, are introduced in the process of preparing calcium hydroxide by adopting a digestion process, so that the prepared calcium-based desulfurizing agent has high desulfurization efficiency in desulfurization treatment of flue gas containing high-concentration carbon dioxide.

The invention provides a rehmannia pigment compound as shown in a formula (I), a stereoisomer thereof or pharmaceutically acceptable salt thereof, and particularly relates to the technical field of natural pharmaceutical chemistry and pharmaceutical compounds. The invention aims to solve the problems that the radix rehmanniae pigment is not clear in material basis and lacks a compound with a clear structure and excellent anti-inflammatory activity. The rehmannia pigment compound disclosed by the invention comprises a structure as shown in a formula (I). The invention also discloses a preparation method of the compound and application of the compound in medicines. The compound has a novel chemical structure and remarkable anti-inflammatory activity, release of nitric oxide can be effectively inhibited in a lipopolysaccharide induced macrophage model, the cytotoxicity is far lower than that of a positive control drug quercetin, and the compound shows huge potential as an anti-inflammatory drug lead compound.

The invention relates to a water-soluble composition as well as a preparation method and application thereof, and belongs to the technical field of daily chemical products. The water-soluble composition disclosed by the invention is prepared from the following components in parts by weight: 2 to 6 parts of paeonol glycoside extract, 0.02 to 0.1 part of licochalcone A, 0.02 to 0.12 part of solubilizer and 0.94 to 4.87 parts of water. According to the water-soluble composition, solubilization is assisted by the paeonol glycoside compound, the addition amount of a solubilizer is greatly reduced, the irritation of the solubilizer is remarkably reduced, and the composition is safe and free of irritation. The water-soluble composition disclosed by the invention can be widely applied to cosmetics such as emulsion and face cream, is particularly suitable for a water agent formula, and can be used for remarkably improving the transparency of a product. Through the synergistic effect of the components, the composition disclosed by the invention has the effects of high transdermal property, efficient moisture retention, immediate soothing and red fading and tissue repair promotion. Meanwhile, the water-soluble composition disclosed by the invention is simple in preparation method, economical and feasible, and wide in market prospect.

The invention belongs to the technical field of medicines, and provides two phenylethanoid glycoside compounds with anti-inflammatory activity in philippine flemingia root as well as a preparation method and application of the two phenylethanoid glycoside compounds. In particular to two novel phenylethanoid glycoside compounds extracted and separated from philippine flemingia root, such as a compound 1 (xylanchoside A) and a compound 2 (xylanchoside B) as shown in structural formulas. The compounds 1-2 have an inhibition effect on cell factors IL-6 and TNF-alpha secreted by LPS-induced macrophages RAW264.7, can form a pharmaceutical composition with a pharmaceutical carrier, and are used for preparing anti-inflammatory drugs.