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476 results about "Fast flow" patented technology

Sea cucumber polypeptide, preparation method and application thereof

The invention discloses a sea cucumber polypeptide, wherein a preparation method thereof comprises the following steps: (1) getting fresh stichopus japonicus, removing entrails, cleaning and crushing the stichopus japonicus into small blocks, and adding water to form homogenate; after the enzymolysis of the homogenate liquid, centrifugating and getting the supernatant; adding ethanol, standing, centrifugating and getting the supernatant, concentrating the supernatant at reducing pressure and drying the concentrate to form a crude product of the sea cucumber polypeptide; (2) dissolving the crude product of the sea cucumber polypeptide by using distilled water, carrying out gel filtration chromatography by Sephadex LH-20, eluting the sea cucumber polypeptide by double-distilled water, getting a second peak, collecting active components, and condensing, freezing and freeze-drying the active components; and (3) dissolving the active components by double-distilled water, carrying out ion exchange chromatography by Q Sepharose Fast Flow, linearly eluting the active components by NaCl solution, desalting, getting the second peak, collecting the active components, and condensing, freeze-drying the active components to form the sea cucumber polypeptide. The sea cucumber polypeptide can be used for preparing medicaments or health-care products for increasing leukocytes and also can be used for preparing medicaments or health-care products for multiplying marrow cells after chemotherapy.
Owner:SHANDONG UNIV
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