The invention discloses a
sea cucumber polypeptide, wherein a preparation method thereof comprises the following steps: (1) getting fresh
stichopus japonicus, removing entrails, cleaning and crushing the
stichopus japonicus into small blocks, and adding water to form homogenate; after the enzymolysis of the homogenate liquid, centrifugating and getting the supernatant; adding
ethanol, standing, centrifugating and getting the supernatant, concentrating the supernatant at reducing pressure and
drying the concentrate to form a crude product of the
sea cucumber polypeptide; (2) dissolving the crude product of the
sea cucumber polypeptide by using
distilled water, carrying out gel
filtration chromatography by
Sephadex LH-20, eluting the sea cucumber polypeptide by double-
distilled water, getting a second peak, collecting
active components, and condensing, freezing and freeze-
drying the
active components; and (3) dissolving the
active components by double-
distilled water, carrying out
ion exchange
chromatography by Q
Sepharose Fast Flow, linearly eluting the active components by NaCl solution, desalting, getting the second peak, collecting the active components, and condensing, freeze-
drying the active components to form the sea cucumber polypeptide. The sea cucumber polypeptide can be used for preparing medicaments or health-care products for increasing leukocytes and also can be used for preparing medicaments or health-care products for multiplying marrow cells after
chemotherapy.