148 results about "Ace inhibitory" patented technology

The invention belongs to the field of bioactive peptides. The invention provides an angiotensin converting enzyme inhibitory peptide as well as a screening method and application thereof. The amino acid sequence of the inhibitory peptide is GKGLW and / or GDGLKW. According to the screening method of the ACE inhibitory peptide, the soybean protein is taken as a raw material, and the ACE inhibitory peptide is prepared and screened by adopting a targeted alkaline protease and chymotrypsin double-enzymolysis technology, so that rapid screening of the ACE inhibitory peptide is realized, and an experimental basis is provided for the structure-function relationship of the ACE inhibitory peptide. The inhibitory peptide provided by the invention has strong antihypertensive activity, high safety and no toxic or side effect. Digestive tract stability determination shows that the inhibitory peptide has good gastrointestinal tract stability in gastrointestinal tract digestion, and can still keep good activity after entering blood.

The invention particularly relates to a method for extracting ACE (Angiotensin Converting Enzyme) inhibitory peptide from tilapia skin. The tilapia skin is a by-product rich in collagen, and has important development significance as a screening raw material of the ACE inhibitor. The invention provides a method for extracting an ACE (Angiotensin Converting Enzyme) inhibitor by taking tilapia skin as a raw material, which comprises the following steps of: degreasing the tilapia skin, crushing, adding acid liquor, extracting to obtain fish skin collagen crude protein, adding enzyme into the fish skin collagen crude protein for enzymolysis, and reserving the part with the molecular weight of less than 3kDa in an enzymolysis product, namely the ACE inhibitory peptide. Particularly, the enzyme is a product of bacillus subtilis or bacillus coagulans under gelatin-induced fermentation.

The invention belongs to the technical field of biological medicines, and discloses an angiotensin I converting enzyme inhibitory peptide. According to the present invention, the action mechanisms of two ACE inhibitory peptides H5 (LIIPQH, 719.85 Da) and H6 (LIIPEH, 720.85 Da) extracted, separated and purified from rice wine residue are researched, and the in vitro activity of the ACE inhibitory peptides is researched through an EA.hy926 cell model, such that the two ACE inhibitory peptides can provide the activity effect by promoting the release of NO, the expression of eNOS and SOD, and inhibiting the release secretion of ET-1 and the release of ROS; meanwhile, the cells are subjected to transcriptomics analysis, and the pathway mechanism of polypeptide action is defined. The ACE inhibitory peptide is expected to serve as a potential antihypertensive drug or a functional food ingredient, the high-value utilization of the rice wine residues is improved, and a new application way is provided for the food-borne ACE inhibitory peptide in the aspect of treating hypertension.

The invention discloses an ACE (Angiotensin Converting Enzyme) inhibition and DPP-IV (Dipeptidyl Peptidase-IV) inhibition bifunctional peptide as well as a design method and application thereof. The amino acid sequence of the bifunctional peptide fragment is Trp-Ala-Leu-Val-Ala-Pro or Trp-Pro-Leu-Val-Ala-Pro. According to the design method, hydrophobic amino acid replacement is carried out on most N-terminal first amino acid according to the characteristics of the DPP-IV inhibitory peptide, and N-terminal second amino acid is kept as proline Pro or alanine Ala. The difunctional peptide fragment obtained through the DPP-IV inhibitory activity optimization strategy has the effects of assisting in lowering blood pressure and lowering blood sugar at the same time and can be used for preparing functional food or health food with the functions of assisting in lowering blood pressure and lowering blood sugar, and the optimization strategy can be applied to improving the blood sugar lowering effect of the peptide fragment and has good market application prospects.

The invention discloses ACE (Angiotensin Converting Enzyme) inhibitory octapeptide YI8 derived from bovine cheese protein hydrolysate and application, and relates to the technical field of bioactive peptides, and the technical scheme is characterized in that the ACE inhibitory octapeptide YI8 comprises an amino acid sequence of YKVPQLEI. The ACE inhibitory octapeptide YI8 derived from the cattle cheese protein hydrolysate is obtained by screening from the cattle cheese protein hydrolysate, and is an octapeptide YI8 with ACE inhibitory activity, the in-vitro ACE inhibition rate of the ACE inhibitory octapeptide YI8 derived from the cattle cheese protein hydrolysate is tested to be 70.86% under the condition of the concentration of 1mg / ml, and the ACE inhibitory octapeptide YI8 is suitable for functional food for assisting in lowering blood pressure.

The invention discloses preparation and application of lotus seed protein peptide with ACE (angiotensin converting enzyme) inhibitory activity, and belongs to the technical field of food-borne active peptide development. According to the ACE inhibitory peptide WVPRYFPF and the preparation method thereof, the ACE inhibitory peptide is rapidly screened by utilizing a polypeptiomics and molecular docking technology, the defects of time consumption and labor consumption caused by step-by-step separation and purification are avoided, the obtained ACE inhibitory peptide WVPRYFPF has extremely high ACE inhibitory activity, IC50 of the ACE inhibitory peptide WVPRYFPF is 16.7 mu M, and the ACE inhibitory peptide WVPRYFPF can be used for assisting or replacing hypertension drugs. The lotus seed protein peptide disclosed by the invention has the characteristic of inhibiting ACE enzyme activity in vitro, and animal experiments prove that the protein peptide can also play a role in lowering blood pressure in a spontaneous hypertension rat (SHR).

The invention relates to the technical field of biology, in particular to an ACE inhibitory peptide, a preparation method and application, the ACE inhibitory peptide has the amino acid sequence of Trp-Pro-Phe (WPF) or Phe-Pro-Trp (FPW) or Phe-Gly-Trp (FGW) or Leu-Pro-Val-Pro-Ala-Phe-Asn (LPVPAFN) or Leu-Trp-Trp-Leu (LWWL), the ACE inhibitory peptide WPF, FPW, FGW, LPVPAFN and LWWL have outstanding antihypertensive activity, the IC50 values of the WPF, FPW, FGW, LPVPAFN and LWWL are 0.264 mg / mL, 0.800 mg / mL, 0.106 mg A variety of aquatic product peptide powder sources are separated and purified by an affinity ultrafiltration screening method, an ultrafiltrate component is selected for amino acid sequence identification, and a new amino acid sequence with ACE inhibitory activity is screened out; the ACE inhibitory peptides WFP, FPW, FGW, LPVPAFN and LWWL prepared by the invention have outstanding antihypertensive activity.

The present invention specifically relates to a method for extracting ACE inhibitory peptides from tilapia skin. Tilapia skin is a byproduct rich in collagen, and has important development significance as a raw material for screening ACE inhibitors. The present invention is designed to provide a method for extracting ACE inhibitors using tilapia skin as a raw material, comprising the following steps: defatting the tilapia skin and crushing it, adding acid to extract to obtain fish skin collagen crude protein, adding enzyme to the fish skin collagen crude protein for enzymolysis, and retaining the part of the enzymolysis product with a molecular weight less than 3kDa is the above-mentioned ACE inhibitory peptide; in particular, the above-mentioned enzyme is a product of Bacillus subtilis or Bacillus coagulans under gelatin-induced fermentation.

The invention discloses ACE (Angiotensin Converting Enzyme) inhibitory decapeptide IR10 derived from bovine cheese protein hydrolysate and application of the ACE inhibitory decapeptide IR10, and relates to the technical field of bioactive peptides, and the technical scheme of the ACE inhibitory decapeptide IR10 is characterized in that the amino acid sequence is IQKEDVPSER. The ACE inhibitory decapeptide IR10 derived from the cattle cheese protein hydrolysate is obtained by screening from the cattle cheese protein hydrolysate and is decapeptide IR10 with ACE inhibitory activity, the in-vitro ACE inhibition rate of the ACE inhibitory decapeptide IR10 derived from the cattle cheese protein hydrolysate is tested to be 62.07% under the condition of the concentration of 1mg / ml, and the ACE inhibitory decapeptide IR10 has the effect of being suitable for functional food for assisting blood pressure reduction.

The invention discloses a cubilose peptide with ACE inhibitory activity and a preparation method and application thereof, the cubilose peptide with the ACE inhibitory activity is prepared through enzymolysis, meanwhile, LC-MS / MS analysis is performed on peptides in enzymatic hydrolysate, a peptide fragment with potential ACE inhibitory activity is screened out in combination with a bioinformatics means, and the obtained peptide fragment has high ACE inhibitory activity through synthesis verification. The bird's nest ACE inhibitory peptide disclosed by the invention has a good application prospect in functional foods or health-care products and food-borne medicines.

The invention provides a spirulina-derived ACE (angiotensin converting enzyme) inhibitory peptide as well as a preparation method and application thereof, and belongs to the technical field of marine bioactive peptides. Spirulina phycobiliprotein is used for preparing the ACE inhibitory peptide of the spirulina source, five kinds of small molecule polypeptides including YATY, RYVTY, YVTY, KAYF and SPSWY are obtained through co-screening, the five kinds of polypeptides all have good ACE inhibitory activity, and the RYVTY activity is the highest. The spirulina-derived polypeptide prepared by the preparation method disclosed by the invention has high hydrolysis degree and high ACE inhibitory activity, can be applied to the fields of food, health care products, medicines and the like, and has a wide application prospect.

The invention discloses a grape seed protein peptide with ACE inhibitory activity and a preparation method thereof, and belongs to the field of comprehensive utilization of wine fermentation byproducts. The invention provides a grape seed protein peptide with ACE (Angiotensin Converting Enzyme) inhibitory activity. The grape seed protein peptide is FFYPL, SGMFPFP or WFFPGPR. The grape seed protein peptide disclosed by the invention can be used for medicines, health-care products or dietary supplements, or can be used as a food base material to be added into common foods such as drinks, dairy products and the like; as used for prevention or auxiliary treatment of hypertensive patients or people suffering from chronic metabolic comprehensive disorder diseases with diseases such as obesity and heart and cerebral vessels, the product has a wide application prospect in the fields of functional foods, medicines, health care products and the like.

The invention discloses a crushing and enzymolysis process for obtaining silkworm chrysalis ACE inhibitory peptide, and belongs to the technical field of protein peptide enzymolysis. Degreased silkworm chrysalis meal is used as a raw material, a raw material stirring and air filtering structure is optimized based on the enzymolysis principle of crushing and stirring the raw material, and in the water phase dissolving process, the enzymolysis efficiency is improved; the degreased silkworm chrysalis meal is efficiently dispersed downwards along the conical surface on the flow guide and gas filtration assembly by utilizing the plurality of liquid injection pipes, and the formed suspension liquid is preheated in the preheating space, so that the enzymolysis reaction of subsequent enzymolysis liquid is favorably improved, and in the enzymolysis reaction process, the stirring assembly periodically rotates, and the stirring efficiency is improved. Multi-point stirring exhaust and top scraping actions are set, so that waste gas in enzymatic hydrolysate is comprehensively and effectively collected, the waste gas is subjected to internal and external dual filtration through a filter disc and a filter material, and viscous solid substances intercepted on the filter disc fall into an enzymolysis space again along with scraping of a stirring assembly to be subjected to an enzymolysis reaction.

The invention relates to a preparation method and application of yolk polypeptide with ACE inhibitory activity, and the method comprises the following steps: Q1, adding degreased yolk powder into a proper amount of water, dissolving, and adjusting the pH value to 6-8; and Q2, adding alkaline protease or flavourzyme to carry out enzymolysis for 1.5-2.5 hours under the conditions that the temperature is 50-60 DEG C and the pH value is 7-8, and then carrying out enzyme deactivation to obtain the egg yolk hydrolysate. The ACE inhibitor with high activity is prepared by adopting step-by-step enzyme hydrolysis, five ACE inhibitory peptides (KFIPL, KFLPGY, KFLPTF, APTFGKL and KLPDMILY) with high activity are screened and identified by utilizing a bioinformatics method, and in order to solve the problem that the polypeptide is unstable after being sterilized in the functional beverage, CMC and GG are used as stabilizers, so that the utilization problem of the enzymolysis polypeptide in the functional beverage is solved.

The invention provides an angiotensin converting enzyme inhibitory peptide and application thereof. The amino acid sequence of the angiotensin converting enzyme inhibitory peptide is PVKP. The ACE inhibitory activity of the inhibitory peptide PVKP provided by the invention is obviously improved compared with that of the original inhibitory peptide LYPVK, and the IC50 value is reduced from 10.6 mu g / mL to 9.6 mu g / mL; the digestive enzyme stability of the inhibitory peptide PVKP provided by the invention and the digestive enzyme stability of the original inhibitory peptide LYPVK under the same concentration are improved. Molecular simulation docking verifies that the interaction force between the ACE inhibitory peptide PVKP and ACE is enhanced compared with that of an original inhibitory peptide LYPVK, and the interaction force between alkyl groups and the interaction between the alkyl groups and Zn < 2 + > of an ACE active center are increased. The intracellular antihypertensive activity of the ACE inhibitory peptide PVKP is further verified through cell experiments, and compared with the original inhibitory peptide LYPVK, the intracellular NO release content is increased, and the ET-1 content is reduced. The ACE inhibitory peptide PVKP is small in molecular weight and good in water solubility, and application of the ACE inhibitory peptide PVKP in development of products with the blood pressure lowering effect is facilitated.

The invention discloses a pressure-controlled bovine bone collagen active peptide, and belongs to the field of food. A potential ACE inhibitory peptide is rapidly screened through a bioinformatics method, the ACE inhibitory activity of the bovine bone collagen peptide is verified in combination with an in-vitro experiment, IC50 values of the screened bovine bone collagen active peptides LGALPGF, VFPGLL and AAAALGPW to ACE are 32.07 mu M, 40.41 mu M and 77.68 mu M respectively, and the peptide with the sequence is proved to have a good antihypertensive function.

The present invention provides Antarctic krill ACE inhibitory peptides VKGVF, FGGAL, WLDAN and their applications, belonging to the technical field of bioactive peptides. The present invention uses defatted Antarctic krill powder to prepare Antarctic krill ACE inhibitory peptides, and the obtained polypeptides have excellent ACE inhibitory activity. Through mass spectrometry identification, a total of 13 small molecule polypeptides were screened, and these 13 polypeptides have different degrees of ACE inhibitory activity. The Antarctic krill ACE inhibitory peptide prepared by the present invention has an ACE inhibitory ability that is dozens or even hundreds of times that of the currently confirmed Antarctic krill ACE inhibitory peptide, and has an excellent ACE inhibitory effect. Antarctic krill ACE inhibitory peptide can be applied to food, health products, medicines and other fields. The present invention makes high-value and precise use of Antarctic krill protein resources, which can effectively improve resource utilization and industrial value, and also provides new ways and methods for preventing and treating hypertension.

The invention discloses a goat milk bioactive polypeptide with antioxidation and angiotensin converting enzyme inhibition activity, and preparation and application thereof, and belongs to the field of biological polypeptides, and a preparation method and application thereof. In-vitro tests prove that the polypeptide has an excellent antioxidant effect, can remarkably reduce blood pressure and is beneficial to prevention and treatment of cardiovascular diseases, the interaction mechanism of the polypeptide and ACE is defined by using a molecular docking technology, scientific basis and targets are provided for research and development of new drugs, and the polypeptide has potential physiological functions such as antioxidant activity and ACE inhibitory activity and has a broad application prospect. The method is suitable for functional food and medicine development and has a wide application prospect.

The invention relates to the technical field of biochemistry, in particular to a specific affinity enrichment and preparation method of a torreya grandis protease hydrolysate ACE inhibitory peptide, which comprises the following steps: step 1, preparing and characterizing magnetic aminated Fe3O4 nanoparticles; step 2, preparing and characterizing the immobilized trypsin; 3, preparing and characterizing the immobilized ACE, and optimizing reaction conditions; step 4, determining the activity of the immobilized ACE; step 5, then preparing and specifically enriching the Chinese torreya antihypertensive peptide; step 6, then, carrying out molecular docking through AutoDock Vina; according to the method, genipin is adopted as a cross-linking agent, magnetic Fe3O4 nanoparticles with the ACE inhibitory peptide specific affinity recognition function are constructed, specific enrichment of the ACE inhibitory peptide from the torreya grandis cake protein hydrolysate is achieved, and specific separation is achieved.

The embodiment of the invention discloses a goat milk-derived ACE (angiotensin converting enzyme) inhibitory peptide monomer as well as a preparation method and application thereof, relates to the technical field of protein separation, and aims to improve a preparation source and preparation efficiency of a food-borne protein-derived ACE inhibitory peptide. The preparation method comprises the following steps: dissolving sheep milk casein in water, and carrying out heating treatment, enzymolysis treatment and freeze drying treatment to obtain a sheep milk source zymolyte; preparing the goat milk source zymolyte into a solution with the concentration of 145mg / mL-155mg / mL, passing through a 0.45 mu m filter membrane, and carrying out primary separation by adopting preparative high performance liquid chromatography to obtain a product Y; preparing the Y product into a solution with the concentration of 20 mg / mL-30 mg / mL, passing through a 0.45 mu m filter membrane, and carrying out secondary separation by adopting preparative high performance liquid chromatography to obtain a Y-X product; preparing the Y-X product into a solution with the concentration of 5mg / mL-15mg / mL, passing through a 0.45 mu m filter membrane, and carrying out re-separation preparation by using a C18 hydrophilic preparative column and preparative high performance liquid chromatography to obtain a product M; the product M is prepared into a solution with the concentration of 5 mg / mL-15 mg / mL, the solution passes through a 0.22-micron filter membrane, after re-separation preparation is conducted through RP-HPLC, rotary evaporation concentration treatment and freeze drying treatment are conducted, and a product F is obtained.

The invention relates to the technical field of biology, in particular to antioxidative peptide and ACE (angiotensin converting enzyme) inhibitory peptide from tuna as well as a preparation method and application of the antioxidative peptide and the ACE inhibitory peptide. The amino acid sequences of the polypeptide provided by the invention are as shown in SEQ ID NO.1-6. The preparation method comprises the following steps: by taking processing waste tuna skin as a raw material, carrying out enzymolysis to obtain tuna skin collagen, then carrying out liquid chromatography-tandem mass spectrometry identification and molecular docking screening on a tuna skin collagen zymolyte, and extracting to obtain polypeptide with antioxidant activity and polypeptide capable of effectively inhibiting ACE (Angiotensin Converting Enzyme) activity. According to the method, the protein extraction step is simplified; meanwhile, on one hand, the deep processing level of the tuna industry can be improved, high-value utilization is achieved, and on the other hand, the bearing pressure of the environment on the processing waste can be reduced.

The invention discloses a yak milk casein ACE (angiotensin converting enzyme) inhibitory peptide as well as a preparation method and application thereof, and relates to the technical field of medicines and health-care products. The preparation method of the yak milk casein ACE inhibitory peptide comprises the following steps: taking yak milk casein as a substrate, adding NaCl and cane sugar to prepare a fermentation culture medium, inoculating a high-yield protease strain into the fermentation culture medium for constant-temperature fermentation, deactivating enzyme after fermentation, centrifugally collecting supernate, and freeze-drying to obtain the yak milk casein ACE inhibitory peptide. The yak milk casein ACE inhibitory peptide oral liquid contains sodium carboxymethyl cellulose, cane sugar, citric acid and yak milk casein ACE inhibitory peptide, the invention also provides an application of the yak milk casein ACE inhibitory peptide in preparation of drugs or health-care products for assisting in lowering blood pressure. The yak milk casein ACE inhibitory peptide disclosed by the invention has an antihypertensive function and good palatability, fills the blank of a yak milk deep processing product market, and provides a technical path for extension of yak milk from a traditional dairy product to the biological medicine field.

The invention discloses a polypeptide with blood pressure lowering and oxidation resisting effects as well as a related product and application thereof, and relates to the technical field of biology. According to the invention, casein is taken as a raw material, and the milk-derived active peptide ALPQY is successfully obtained through multi-enzyme hydrolysis, separation, purification and identification. In-vitro activity evaluation results show that the polypeptide not only has strong ACE inhibitory activity, but also can effectively scavenge free radicals; the ACE inhibition IC50 value is 35.56 mu M, the free radical scavenging TEAC value is 1.83 mu mol TE / mu mol, and compared with similar polypeptides, the polypeptide has better ACE inhibition effect and free radical scavenging effect. In-vivo antihypertensive evaluation is carried out through a spontaneous hypertension rat model, it is also shown that the compound has a good antihypertensive effect, and the compound can be used for preparing products related to antihypertensive and / or antioxidation.

The invention belongs to the technical field of wine brewing, and particularly relates to health-preserving highland barley wine and a preparation method thereof. Appropriate fermentation temperature, fermentation time and distiller's yeast addition amount are determined, so that the mouth feel of the health-preserving highland barley wine is stable, and high-quality and standardized processing of the highland barley wine is realized. The health-preserving highland barley wine prepared by the method disclosed by the invention has good ACE inhibitory activity.

The invention provides a method for screening and verifying kelp chloroplast prosthesis protein ACE (angiotensin converting enzyme) inhibitory peptide, which comprises the following two steps of: 1, carrying out computer-assisted enzymolysis on kelp protein, selecting a representative protein sequence in kelp by using an online database, selecting specific protease by using an online enzymolysis platform to carry out virtual enzymolysis, and screening and verifying the ACE inhibitory peptide according to an enzymolysis result; establishing a kelp protein polypeptide classification database; and step 2, establishing a QSAR model of the ACE inhibitory active peptide, and predicting the activity of the ACE inhibitory active peptide, so that the invention provides a research and development method system for a functional product rich in the ACE inhibitory active peptide based on the combination of kelp bioinformatics and experimental verification.

The invention discloses a mulberry leaf antihypertensive active peptide as well as a preparation method and application thereof. According to the method, mulberry leaves are used as raw materials to prepare mulberry leaf albumin, enzymolysis parameters of enzymolysis time, pH, enzyme-substrate ratio and substrate concentration are optimized through a single factor experiment and an orthogonal experiment, and optimal enzymolysis conditions for preparing the ACE inhibitory peptide crude product are provided; the high-activity ACE inhibitory peptide is identified through further purification, polypeptide sequence determination and virtual screening, the ACE inhibitory peptide has extremely high solubility and good ACE inhibitory activity, and the IC50 values of the two peptides VPSCFDLTGK and RLPDFHGL with the highest activity are 8.23 [mu] mol / L (8.765 [mu] g / mL) and 23.01 [mu] mol / L (23.58 [mu] g / mL) respectively. The active peptide can be used as an angiotensin converting enzyme inhibitory peptide, and achieves the purpose of reducing blood pressure by inhibiting ACE activity.

The objective of the present invention is to provide a peptide having ACE inhibitory activity and a composition thereof. [Solution] The present invention discloses a peptide with ACE inhibitory activity and a composition thereof. The peptide is composed of three amino acids and has the ability to inhibit ACE activity. Therefore, by administering an effective amount of the peptide disclosed in the present invention or a composition containing the peptide to an individual, the individual's blood pressure can be effectively adjusted to approach normal values, thereby achieving the effect of preventing and / or treating hypertension and its related diseases.

This invention discloses a grape seed protein peptide with ACE inhibitory activity and its preparation method, belonging to the field of comprehensive utilization of wine fermentation by-products. This invention provides a grape seed protein peptide with ACE inhibitory activity, wherein the grape seed protein peptide is FFYPL, SGMFPFP, or WFFPGPR. The grape seed protein peptide of this invention can be used in pharmaceuticals; it has broad application prospects in the pharmaceutical field as a preventive or adjunctive treatment for patients with hypertension, or those with obesity, cardiovascular and cerebrovascular diseases, and other chronic metabolic disorders.

The invention provides pacific Dosidicus gigas visceral mass ACE (angiotensin converting enzyme) inhibitory peptide as well as a preparation method and application thereof, and belongs to the technical field of marine bioactive peptides. The high-activity ACE inhibitory peptide is prepared from the visceral mass of Dosidicus gigas in Pacific Ocean, and the obtained polypeptide has excellent ACE inhibitory activity. Through mass spectrum identification, eight kinds of small molecule polypeptides are obtained through co-screening, and the eight kinds of polypeptides have different degrees of ACE inhibitory activity. The ACE inhibition capability of the gibberella pacificus visceral mass ACE inhibitory peptide prepared by the invention is several times or even dozens of times of the ACE inhibition capability of the gibberella pacificus visceral mass protein peptide confirmed at present, and the gibberella pacificus visceral mass ACE inhibitory peptide has an excellent ACE inhibition effect. According to the method disclosed by the invention, the processing byproduct resource, namely the visceral mass of Dosidicus gigas in Pacific Ocean, is accurately utilized at a high value, so that the resource utilization rate and the industrial value can be effectively improved, and meanwhile, a new way and a new method are provided for preventing and treating hypertension.

The invention discloses an ACE (angiotensin converting enzyme) inhibitory peptide with antihypertensive activity and application thereof, and belongs to the technical field of active peptide biology, amino acid sequences of the ACE inhibitory peptide are EYPVK and SYPVK, the two peptides are obtained through module replacement design, the ACE inhibitory activity of the two peptides is remarkably improved compared with that of an original peptide LYPVK, IC50 values are respectively reduced to 14.82 + / -0.82 mu M and 12.83 + / -0.79 mu M from 117.14 + / -1.40 mu M, and the inhibitory activity is improved by 13.5% and 25.1%. Molecular simulation analysis shows that the interaction force between the two and ACE is stronger, and a potential modular effect exists between sequences. Besides, the polypeptide has remarkable blood pressure lowering activity on human umbilical vein endothelial cells, plays a role by regulating release of NO and ET-1, has the activity superior to that of original peptides, has the advantages of good digestive enzyme stability, small molecular weight and good water solubility, and is suitable for developing related products for lowering blood pressure.