118 results about "Ace inhibitory" patented technology

The invention discloses ACE (Angiotensin Converting Enzyme) inhibitory octapeptide YI8 derived from bovine cheese protein hydrolysate and application, and relates to the technical field of bioactive peptides, and the technical scheme is characterized in that the ACE inhibitory octapeptide YI8 comprises an amino acid sequence of YKVPQLEI. The ACE inhibitory octapeptide YI8 derived from the cattle cheese protein hydrolysate is obtained by screening from the cattle cheese protein hydrolysate, and is an octapeptide YI8 with ACE inhibitory activity, the in-vitro ACE inhibition rate of the ACE inhibitory octapeptide YI8 derived from the cattle cheese protein hydrolysate is tested to be 70.86% under the condition of the concentration of 1mg / ml, and the ACE inhibitory octapeptide YI8 is suitable for functional food for assisting in lowering blood pressure.

The invention relates to the technical field of biology, in particular to an ACE inhibitory peptide, a preparation method and application, the ACE inhibitory peptide has the amino acid sequence of Trp-Pro-Phe (WPF) or Phe-Pro-Trp (FPW) or Phe-Gly-Trp (FGW) or Leu-Pro-Val-Pro-Ala-Phe-Asn (LPVPAFN) or Leu-Trp-Trp-Leu (LWWL), the ACE inhibitory peptide WPF, FPW, FGW, LPVPAFN and LWWL have outstanding antihypertensive activity, the IC50 values of the WPF, FPW, FGW, LPVPAFN and LWWL are 0.264 mg / mL, 0.800 mg / mL, 0.106 mg A variety of aquatic product peptide powder sources are separated and purified by an affinity ultrafiltration screening method, an ultrafiltrate component is selected for amino acid sequence identification, and a new amino acid sequence with ACE inhibitory activity is screened out; the ACE inhibitory peptides WFP, FPW, FGW, LPVPAFN and LWWL prepared by the invention have outstanding antihypertensive activity.

The present invention specifically relates to a method for extracting ACE inhibitory peptides from tilapia skin. Tilapia skin is a byproduct rich in collagen, and has important development significance as a raw material for screening ACE inhibitors. The present invention is designed to provide a method for extracting ACE inhibitors using tilapia skin as a raw material, comprising the following steps: defatting the tilapia skin and crushing it, adding acid to extract to obtain fish skin collagen crude protein, adding enzyme to the fish skin collagen crude protein for enzymolysis, and retaining the part of the enzymolysis product with a molecular weight less than 3kDa is the above-mentioned ACE inhibitory peptide; in particular, the above-mentioned enzyme is a product of Bacillus subtilis or Bacillus coagulans under gelatin-induced fermentation.

The invention discloses ACE (Angiotensin Converting Enzyme) inhibitory decapeptide IR10 derived from bovine cheese protein hydrolysate and application of the ACE inhibitory decapeptide IR10, and relates to the technical field of bioactive peptides, and the technical scheme of the ACE inhibitory decapeptide IR10 is characterized in that the amino acid sequence is IQKEDVPSER. The ACE inhibitory decapeptide IR10 derived from the cattle cheese protein hydrolysate is obtained by screening from the cattle cheese protein hydrolysate and is decapeptide IR10 with ACE inhibitory activity, the in-vitro ACE inhibition rate of the ACE inhibitory decapeptide IR10 derived from the cattle cheese protein hydrolysate is tested to be 62.07% under the condition of the concentration of 1mg / ml, and the ACE inhibitory decapeptide IR10 has the effect of being suitable for functional food for assisting blood pressure reduction.

The invention provides a spirulina-derived ACE (angiotensin converting enzyme) inhibitory peptide as well as a preparation method and application thereof, and belongs to the technical field of marine bioactive peptides. Spirulina phycobiliprotein is used for preparing the ACE inhibitory peptide of the spirulina source, five kinds of small molecule polypeptides including YATY, RYVTY, YVTY, KAYF and SPSWY are obtained through co-screening, the five kinds of polypeptides all have good ACE inhibitory activity, and the RYVTY activity is the highest. The spirulina-derived polypeptide prepared by the preparation method disclosed by the invention has high hydrolysis degree and high ACE inhibitory activity, can be applied to the fields of food, health care products, medicines and the like, and has a wide application prospect.

The invention discloses a grape seed protein peptide with ACE inhibitory activity and a preparation method thereof, and belongs to the field of comprehensive utilization of wine fermentation byproducts. The invention provides a grape seed protein peptide with ACE (Angiotensin Converting Enzyme) inhibitory activity. The grape seed protein peptide is FFYPL, SGMFPFP or WFFPGPR. The grape seed protein peptide disclosed by the invention can be used for medicines, health-care products or dietary supplements, or can be used as a food base material to be added into common foods such as drinks, dairy products and the like; as used for prevention or auxiliary treatment of hypertensive patients or people suffering from chronic metabolic comprehensive disorder diseases with diseases such as obesity and heart and cerebral vessels, the product has a wide application prospect in the fields of functional foods, medicines, health care products and the like.

The invention discloses a crushing and enzymolysis process for obtaining silkworm chrysalis ACE inhibitory peptide, and belongs to the technical field of protein peptide enzymolysis. Degreased silkworm chrysalis meal is used as a raw material, a raw material stirring and air filtering structure is optimized based on the enzymolysis principle of crushing and stirring the raw material, and in the water phase dissolving process, the enzymolysis efficiency is improved; the degreased silkworm chrysalis meal is efficiently dispersed downwards along the conical surface on the flow guide and gas filtration assembly by utilizing the plurality of liquid injection pipes, and the formed suspension liquid is preheated in the preheating space, so that the enzymolysis reaction of subsequent enzymolysis liquid is favorably improved, and in the enzymolysis reaction process, the stirring assembly periodically rotates, and the stirring efficiency is improved. Multi-point stirring exhaust and top scraping actions are set, so that waste gas in enzymatic hydrolysate is comprehensively and effectively collected, the waste gas is subjected to internal and external dual filtration through a filter disc and a filter material, and viscous solid substances intercepted on the filter disc fall into an enzymolysis space again along with scraping of a stirring assembly to be subjected to an enzymolysis reaction.

The invention relates to a preparation method and application of yolk polypeptide with ACE inhibitory activity, and the method comprises the following steps: Q1, adding degreased yolk powder into a proper amount of water, dissolving, and adjusting the pH value to 6-8; and Q2, adding alkaline protease or flavourzyme to carry out enzymolysis for 1.5-2.5 hours under the conditions that the temperature is 50-60 DEG C and the pH value is 7-8, and then carrying out enzyme deactivation to obtain the egg yolk hydrolysate. The ACE inhibitor with high activity is prepared by adopting step-by-step enzyme hydrolysis, five ACE inhibitory peptides (KFIPL, KFLPGY, KFLPTF, APTFGKL and KLPDMILY) with high activity are screened and identified by utilizing a bioinformatics method, and in order to solve the problem that the polypeptide is unstable after being sterilized in the functional beverage, CMC and GG are used as stabilizers, so that the utilization problem of the enzymolysis polypeptide in the functional beverage is solved.

The invention discloses a pressure-controlled bovine bone collagen active peptide, and belongs to the field of food. A potential ACE inhibitory peptide is rapidly screened through a bioinformatics method, the ACE inhibitory activity of the bovine bone collagen peptide is verified in combination with an in-vitro experiment, IC50 values of the screened bovine bone collagen active peptides LGALPGF, VFPGLL and AAAALGPW to ACE are 32.07 mu M, 40.41 mu M and 77.68 mu M respectively, and the peptide with the sequence is proved to have a good antihypertensive function.

The invention relates to the technical field of biochemistry, in particular to a specific affinity enrichment and preparation method of a torreya grandis protease hydrolysate ACE inhibitory peptide, which comprises the following steps: step 1, preparing and characterizing magnetic aminated Fe3O4 nanoparticles; step 2, preparing and characterizing the immobilized trypsin; 3, preparing and characterizing the immobilized ACE, and optimizing reaction conditions; step 4, determining the activity of the immobilized ACE; step 5, then preparing and specifically enriching the Chinese torreya antihypertensive peptide; step 6, then, carrying out molecular docking through AutoDock Vina; according to the method, genipin is adopted as a cross-linking agent, magnetic Fe3O4 nanoparticles with the ACE inhibitory peptide specific affinity recognition function are constructed, specific enrichment of the ACE inhibitory peptide from the torreya grandis cake protein hydrolysate is achieved, and specific separation is achieved.

The embodiment of the invention discloses a goat milk-derived ACE (angiotensin converting enzyme) inhibitory peptide monomer as well as a preparation method and application thereof, relates to the technical field of protein separation, and aims to improve a preparation source and preparation efficiency of a food-borne protein-derived ACE inhibitory peptide. The preparation method comprises the following steps: dissolving sheep milk casein in water, and carrying out heating treatment, enzymolysis treatment and freeze drying treatment to obtain a sheep milk source zymolyte; preparing the goat milk source zymolyte into a solution with the concentration of 145mg / mL-155mg / mL, passing through a 0.45 mu m filter membrane, and carrying out primary separation by adopting preparative high performance liquid chromatography to obtain a product Y; preparing the Y product into a solution with the concentration of 20 mg / mL-30 mg / mL, passing through a 0.45 mu m filter membrane, and carrying out secondary separation by adopting preparative high performance liquid chromatography to obtain a Y-X product; preparing the Y-X product into a solution with the concentration of 5mg / mL-15mg / mL, passing through a 0.45 mu m filter membrane, and carrying out re-separation preparation by using a C18 hydrophilic preparative column and preparative high performance liquid chromatography to obtain a product M; the product M is prepared into a solution with the concentration of 5 mg / mL-15 mg / mL, the solution passes through a 0.22-micron filter membrane, after re-separation preparation is conducted through RP-HPLC, rotary evaporation concentration treatment and freeze drying treatment are conducted, and a product F is obtained.

The invention discloses a yak milk casein ACE (angiotensin converting enzyme) inhibitory peptide as well as a preparation method and application thereof, and relates to the technical field of medicines and health-care products. The preparation method of the yak milk casein ACE inhibitory peptide comprises the following steps: taking yak milk casein as a substrate, adding NaCl and cane sugar to prepare a fermentation culture medium, inoculating a high-yield protease strain into the fermentation culture medium for constant-temperature fermentation, deactivating enzyme after fermentation, centrifugally collecting supernate, and freeze-drying to obtain the yak milk casein ACE inhibitory peptide. The yak milk casein ACE inhibitory peptide oral liquid contains sodium carboxymethyl cellulose, cane sugar, citric acid and yak milk casein ACE inhibitory peptide, the invention also provides an application of the yak milk casein ACE inhibitory peptide in preparation of drugs or health-care products for assisting in lowering blood pressure. The yak milk casein ACE inhibitory peptide disclosed by the invention has an antihypertensive function and good palatability, fills the blank of a yak milk deep processing product market, and provides a technical path for extension of yak milk from a traditional dairy product to the biological medicine field.

The invention discloses a polypeptide with blood pressure lowering and oxidation resisting effects as well as a related product and application thereof, and relates to the technical field of biology. According to the invention, casein is taken as a raw material, and the milk-derived active peptide ALPQY is successfully obtained through multi-enzyme hydrolysis, separation, purification and identification. In-vitro activity evaluation results show that the polypeptide not only has strong ACE inhibitory activity, but also can effectively scavenge free radicals; the ACE inhibition IC50 value is 35.56 mu M, the free radical scavenging TEAC value is 1.83 mu mol TE / mu mol, and compared with similar polypeptides, the polypeptide has better ACE inhibition effect and free radical scavenging effect. In-vivo antihypertensive evaluation is carried out through a spontaneous hypertension rat model, it is also shown that the compound has a good antihypertensive effect, and the compound can be used for preparing products related to antihypertensive and / or antioxidation.

The invention discloses a mulberry leaf antihypertensive active peptide as well as a preparation method and application thereof. According to the method, mulberry leaves are used as raw materials to prepare mulberry leaf albumin, enzymolysis parameters of enzymolysis time, pH, enzyme-substrate ratio and substrate concentration are optimized through a single factor experiment and an orthogonal experiment, and optimal enzymolysis conditions for preparing the ACE inhibitory peptide crude product are provided; the high-activity ACE inhibitory peptide is identified through further purification, polypeptide sequence determination and virtual screening, the ACE inhibitory peptide has extremely high solubility and good ACE inhibitory activity, and the IC50 values of the two peptides VPSCFDLTGK and RLPDFHGL with the highest activity are 8.23 [mu] mol / L (8.765 [mu] g / mL) and 23.01 [mu] mol / L (23.58 [mu] g / mL) respectively. The active peptide can be used as an angiotensin converting enzyme inhibitory peptide, and achieves the purpose of reducing blood pressure by inhibiting ACE activity.

The objective of the present invention is to provide a peptide having ACE inhibitory activity and a composition thereof. [Solution] The present invention discloses a peptide with ACE inhibitory activity and a composition thereof. The peptide is composed of three amino acids and has the ability to inhibit ACE activity. Therefore, by administering an effective amount of the peptide disclosed in the present invention or a composition containing the peptide to an individual, the individual's blood pressure can be effectively adjusted to approach normal values, thereby achieving the effect of preventing and / or treating hypertension and its related diseases.

This invention discloses a grape seed protein peptide with ACE inhibitory activity and its preparation method, belonging to the field of comprehensive utilization of wine fermentation by-products. This invention provides a grape seed protein peptide with ACE inhibitory activity, wherein the grape seed protein peptide is FFYPL, SGMFPFP, or WFFPGPR. The grape seed protein peptide of this invention can be used in pharmaceuticals; it has broad application prospects in the pharmaceutical field as a preventive or adjunctive treatment for patients with hypertension, or those with obesity, cardiovascular and cerebrovascular diseases, and other chronic metabolic disorders.

The invention discloses an ACE (angiotensin converting enzyme) inhibitory peptide with antihypertensive activity and application thereof, and belongs to the technical field of active peptide biology, amino acid sequences of the ACE inhibitory peptide are EYPVK and SYPVK, the two peptides are obtained through module replacement design, the ACE inhibitory activity of the two peptides is remarkably improved compared with that of an original peptide LYPVK, IC50 values are respectively reduced to 14.82 + / -0.82 mu M and 12.83 + / -0.79 mu M from 117.14 + / -1.40 mu M, and the inhibitory activity is improved by 13.5% and 25.1%. Molecular simulation analysis shows that the interaction force between the two and ACE is stronger, and a potential modular effect exists between sequences. Besides, the polypeptide has remarkable blood pressure lowering activity on human umbilical vein endothelial cells, plays a role by regulating release of NO and ET-1, has the activity superior to that of original peptides, has the advantages of good digestive enzyme stability, small molecular weight and good water solubility, and is suitable for developing related products for lowering blood pressure.

The present application relates to the technical field of biology, and particularly relates to an antioxidant peptide and ACE inhibitory peptide from tuna, and a preparation method and application thereof. The amino acid sequence of the polypeptide provided by the present application is shown as SEQ ID NO. 1-6. The present application takes tuna skin waste as raw material, obtains tuna skin collagen through enzymolysis, then performs liquid chromatography-tandem mass spectrometry identification and molecular docking screening on the tuna skin collagen enzymolysis product, and obtains polypeptides with antioxidant activity and polypeptides effectively inhibiting ACE activity. The method simplifies the protein extraction step. Meanwhile, the method can improve the deep processing level of the tuna industry, achieve high-value utilization, and reduce the environmental pressure on processing waste.

The application discloses wrinkle abalone visceral-derived antioxidant peptides and ACE inhibiting peptides, and a preparation method and application thereof. 50 With DPPH free radical scavenging activity and ACE inhibiting activity as the guidance, the enzymatic hydrolysate is separated and purified through a series of methods such as ultrafiltration, dextran gel chromatography and high performance liquid chromatography, and is combined with bioinformatics screening to obtain one DPPH free radical scavenging activity peptide and three ACE inhibiting peptides, the amino acid sequences of which are shown as SEQ ID NO. 1-4, and the IC 50 values are 3.05 mg / mL, 77.43 μM, 143.93 μM and 124.10 μM respectively, and the four polypeptides have great application value. The application realizes high-value utilization of abalone viscera, and provides a reliable basis for application of abalone by-products.

The invention relates to a preparation method of a rice bran protein ACE inhibitory peptide, and belongs to the technical field of bioactive peptide preparation. The preparation method comprises the following steps: extracting rice bran protein from defatted rice bran, carrying out step-by-step enzymolysis by using alkaline protease and papain to obtain a rice bran protein ACE crude peptide solution, centrifuging, carrying out ultrafiltration by using a 3kDa ultrafiltration tube, and purifying by using gel chromatography to obtain the rice bran protein ACE inhibitory peptide. The process is simple and efficient, the cost is controllable, the obtained ACE inhibitory peptide not only has high ACE inhibitory activity, but also has high oxidation resistance, is rich in various bioactive amino acids, can be used for efficiently preparing active peptide with strong antihypertensive potential, and provides an ideal raw material for developing novel antihypertensive food, medicine and health care products.

This invention discloses a chicken gizzard membrane peptide with ACE inhibitory activity, its preparation method, and its application, belonging to the field of bioactive peptide technology. This chicken gizzard membrane peptide is a tetrapeptide with the amino acid sequence SRVW and a molecular weight of 546.29143 Da, exhibiting significant ACE inhibitory activity. The advantages of this invention are: (1) it is derived from chicken gizzard membrane, a traditional Chinese medicine, and is a natural ACE inhibitory peptide, making it safer for the human body; (2) its small molecular weight makes it easier for the human body to absorb; and (3) it inhibits ACE at IC50 levels. 50 With a value of 0.022 mM (0.012 mg / mL), it exhibits significant ACE inhibitory activity and has a higher antihypertensive activity than most existing ACE inhibitory peptides. It can be used to prepare antihypertensive drugs or compositions (for treating hypertension) and antihypertensive foods (for preventing hypertension), and has important application value and market prospects in the development of antihypertensive functional products.

The invention discloses a multifunctional active peptide from mackerel viscera and application of the multifunctional active peptide. The mackerel viscera extract is prepared by adopting a single exogenous enzyme hydrolysis method, the extract has small molecule peptides with ACE inhibitory activity, three ACE inhibitory peptides are obtained by further identification and screening, the amino acid sequences are respectively GPPVPGP, GPMGPMGP and SPGPVGMP, and the half inhibitory concentration IC50 values of the ACE inhibitory peptides are respectively 80.84 + / -1.12 mu g / mL, 177.41 + / -3.26 mu g / mL and 163.25 + / -3.46 mu g / mL. Wherein the heptapeptide GPPVPGP with the optimal activity also has the function of removing DPPH free radicals and hydroxyl free radicals, and can be used for adjuvant therapy for reducing hypertension or applied to other fields.

This invention belongs to the field of food biotechnology and bioactive peptides, and specifically relates to a cottonseed protein-derived ACE-inhibiting peptide, its screening, modification methods, and applications. The screening method of this application includes four core steps: virtual enzymatic library construction, multi-stage computer filtering, molecular docking screening, and in vitro activity verification, obtaining 194 high-confidence candidate peptides. Four cottonseed protein-derived peptides (SYF, DIF, IAF, and RFY) with in vitro ACE-inhibiting activity were verified, all exhibiting mixed ACE-inhibiting activity, with DIF showing the strongest inhibitory activity. Molecular dynamics simulations confirmed the structural stability of the ACE-DIF complex. A modified peptide FIF was obtained through a dipeptide module substitution strategy, with an IC50 value of [missing information]. 50 The activity was significantly reduced, approximately 3.7 times that of the parent peptide DIF, and the inhibition type changed from mixed to competitive. This invention provides a new approach for the development of natural antihypertensive functional peptides and the high-value utilization of plant protein resources.

The application discloses a pentapeptide ES5 with ACE inhibitory activity and a preparation method and application thereof, and belongs to the technical field of biotechnology.The amino acid sequence of the pentapeptide ES5 is ERQFS, and the pentapeptide ES5 can be prepared by a solid-phase synthesis method and an enzymatic hydrolysis method.The pentapeptide ES5 is identified from a protease hydrolysate of red algae (real Gracilaria) agar residue, has potential interaction with ACE, and has an ACE inhibition rate of 90.84% at a concentration of 0.1 mg / mL; compared with VPP and IPP (the ACE inhibition rates are 82.32% and 78.00% respectively at the same concentration), the ACE inhibitory activity is extremely significantly increased (p<0.001); and the pentapeptide ES5 can be used for preparing antihypertensive drugs or antihypertensive functional food.

The present application relates to a kind of preparation method and application of egg yolk polypeptide with ACE inhibitory activity, the method comprises: Q1.degreased egg yolk powder is dissolved in appropriate amount of water, adjust pH to 6~8;Q2.50~60 ℃, pH=7‑8 under the condition of adding alkaline protease or flavour protease is carried out enzymolysis 1.5~2.5h, then enzyme is killed, and the egg yolk hydrolysate is obtained.The present application not only uses step-by-step enzymatic hydrolysis to prepare high-activity ACE inhibitor, and using the method of bioinformatics, 5 ACE inhibitory peptides (KFIPL, KFLPGY, KFLPTF, ATPFGKL, KLPDMILY) with high activity are screened and identified, and in order to solve the problem of polypeptide instability after sterilization in functional beverage, CMC and GG are used as stabilizer, and the problem of enzymatic polypeptide in functional beverage is solved.

The invention belongs to the field of medicines, and particularly relates to a bacterial-derived ACE inhibitory peptide and application thereof. The ACE inhibitor is a novel peptide, is named as PVQRFF, has remarkable ACE inhibitory activity, and can effectively reduce the level of angiotensin II so as to achieve the purpose of controlling blood pressure. The PVQRFF has the potential application value in hypertension prevention and treatment by reducing blood pressure, improving blood circulation and protecting the cardiovascular system.

The application provides a polypeptide with angiotensin converting enzyme inhibitory activity and a preparation method and application thereof, and relates to the technical field of active peptide preparation. The abalone is pulverized, a complex enzyme is added for primary enzymolysis, and then a specific protease mixture containing prolyl endopeptidase, chymotrypsin and protease K is added for two-step enzymolysis, so that the obtained polypeptide solution has high ACE inhibitory activity. The obtained polypeptide is analyzed and screened to obtain polypeptides with amino acid sequences as shown in SEQ ID NO. 1-5. The polypeptide has high ACE inhibitory activity, and the IC 50 0.05mg / mL-0.40mg / mL, and therefore can play a blood pressure lowering effect as an angiotensin converting enzyme inhibitor.

The invention discloses an active peptide with antihypertensive activity and a preparation method and application thereof, and relates to the technical field of dairy products. A novel active peptide is obtained through screening, and the amino acid sequence of the novel active peptide is LDAYPGSAW. Through in-vitro activity evaluation, the polypeptide not only has relatively strong ACE inhibitory activity, but also has relatively strong oxidation resistance, the IC50 value for inhibiting ACE is 62.37 [mu] M, and the TEAC value for scavenging free radicals is 2.10 [mu] mol TE / [mu] mol. In-vivo blood pressure reduction evaluation is carried out through a spontaneous hypertension rat model, and it is shown that the compound also has an excellent blood pressure reduction effect in vivo and can be applied to preparation of products related to blood pressure reduction and / or oxidation resistance.

The invention relates to the technical field of functional food processing, in particular to a sweet almond ACE (angiotensin converting enzyme) inhibitory peptide as well as a composition, a preparation method and application thereof. The invention provides a sweet almond ACE inhibitory peptide. The amino acid sequence of the sweet almond ACE inhibitory peptide is shown as SEQ ID NO: 1. The sweet almond ACE inhibitory peptide composition with high yield and strong activity is prepared by taking cold-pressed sweet almond meal as a test material, adopting immobilized enzyme and optimizing an enzymolysis process. Amino acid sequence analysis is carried out on sweet almond ACE inhibitory peptide components of 1-3kDa components by adopting an LC-TOFMS (Liquid Chromatography-Time of Flight Mass Spectrometry) method, most hydrogen bonds are formed between screened polypeptide with an amino acid sequence shown as SEQ ID NO: 1 and key active sites of ACE, and a better ACE inhibitory effect is shown.

The invention relates to the technical field of food and / or medicine, and provides a cicada pupa small-molecule active peptide and a preparation method and application thereof.The preparation method comprises the steps that cicada pupa serves as a raw material, and the small-molecule active peptide is prepared through extraction, enzymolysis, macroporous resin separation and sephadex separation; the obtained small molecule active peptide has remarkable ACE inhibitory activity and good antioxidant activity, and shows huge application potential in the fields of drug development, health care product development, functional food addition and the like.