52 results about "Ace inhibitory" patented technology

The invention discloses a yak milk casein ACE (angiotensin converting enzyme) inhibitory peptide as well as a preparation method and application thereof, and relates to the technical field of medicines and health-care products. The preparation method of the yak milk casein ACE inhibitory peptide comprises the following steps: taking yak milk casein as a substrate, adding NaCl and cane sugar to prepare a fermentation culture medium, inoculating a high-yield protease strain into the fermentation culture medium for constant-temperature fermentation, deactivating enzyme after fermentation, centrifugally collecting supernate, and freeze-drying to obtain the yak milk casein ACE inhibitory peptide. The yak milk casein ACE inhibitory peptide oral liquid contains sodium carboxymethyl cellulose, cane sugar, citric acid and yak milk casein ACE inhibitory peptide, the invention also provides an application of the yak milk casein ACE inhibitory peptide in preparation of drugs or health-care products for assisting in lowering blood pressure. The yak milk casein ACE inhibitory peptide disclosed by the invention has an antihypertensive function and good palatability, fills the blank of a yak milk deep processing product market, and provides a technical path for extension of yak milk from a traditional dairy product to the biological medicine field.

The invention discloses a polypeptide with blood pressure lowering and oxidation resisting effects as well as a related product and application thereof, and relates to the technical field of biology. According to the invention, casein is taken as a raw material, and the milk-derived active peptide ALPQY is successfully obtained through multi-enzyme hydrolysis, separation, purification and identification. In-vitro activity evaluation results show that the polypeptide not only has strong ACE inhibitory activity, but also can effectively scavenge free radicals; the ACE inhibition IC50 value is 35.56 mu M, the free radical scavenging TEAC value is 1.83 mu mol TE / mu mol, and compared with similar polypeptides, the polypeptide has better ACE inhibition effect and free radical scavenging effect. In-vivo antihypertensive evaluation is carried out through a spontaneous hypertension rat model, it is also shown that the compound has a good antihypertensive effect, and the compound can be used for preparing products related to antihypertensive and / or antioxidation.

This invention discloses a grape seed protein peptide with ACE inhibitory activity and its preparation method, belonging to the field of comprehensive utilization of wine fermentation by-products. This invention provides a grape seed protein peptide with ACE inhibitory activity, wherein the grape seed protein peptide is FFYPL, SGMFPFP, or WFFPGPR. The grape seed protein peptide of this invention can be used in pharmaceuticals; it has broad application prospects in the pharmaceutical field as a preventive or adjunctive treatment for patients with hypertension, or those with obesity, cardiovascular and cerebrovascular diseases, and other chronic metabolic disorders.

The invention discloses an ACE (angiotensin converting enzyme) inhibitory peptide with antihypertensive activity and application thereof, and belongs to the technical field of active peptide biology, amino acid sequences of the ACE inhibitory peptide are EYPVK and SYPVK, the two peptides are obtained through module replacement design, the ACE inhibitory activity of the two peptides is remarkably improved compared with that of an original peptide LYPVK, IC50 values are respectively reduced to 14.82 + / -0.82 mu M and 12.83 + / -0.79 mu M from 117.14 + / -1.40 mu M, and the inhibitory activity is improved by 13.5% and 25.1%. Molecular simulation analysis shows that the interaction force between the two and ACE is stronger, and a potential modular effect exists between sequences. Besides, the polypeptide has remarkable blood pressure lowering activity on human umbilical vein endothelial cells, plays a role by regulating release of NO and ET-1, has the activity superior to that of original peptides, has the advantages of good digestive enzyme stability, small molecular weight and good water solubility, and is suitable for developing related products for lowering blood pressure.

The invention relates to a preparation method of a rice bran protein ACE inhibitory peptide, and belongs to the technical field of bioactive peptide preparation. The preparation method comprises the following steps: extracting rice bran protein from defatted rice bran, carrying out step-by-step enzymolysis by using alkaline protease and papain to obtain a rice bran protein ACE crude peptide solution, centrifuging, carrying out ultrafiltration by using a 3kDa ultrafiltration tube, and purifying by using gel chromatography to obtain the rice bran protein ACE inhibitory peptide. The process is simple and efficient, the cost is controllable, the obtained ACE inhibitory peptide not only has high ACE inhibitory activity, but also has high oxidation resistance, is rich in various bioactive amino acids, can be used for efficiently preparing active peptide with strong antihypertensive potential, and provides an ideal raw material for developing novel antihypertensive food, medicine and health care products.

The invention discloses a multifunctional active peptide from mackerel viscera and application of the multifunctional active peptide. The mackerel viscera extract is prepared by adopting a single exogenous enzyme hydrolysis method, the extract has small molecule peptides with ACE inhibitory activity, three ACE inhibitory peptides are obtained by further identification and screening, the amino acid sequences are respectively GPPVPGP, GPMGPMGP and SPGPVGMP, and the half inhibitory concentration IC50 values of the ACE inhibitory peptides are respectively 80.84 + / -1.12 mu g / mL, 177.41 + / -3.26 mu g / mL and 163.25 + / -3.46 mu g / mL. Wherein the heptapeptide GPPVPGP with the optimal activity also has the function of removing DPPH free radicals and hydroxyl free radicals, and can be used for adjuvant therapy for reducing hypertension or applied to other fields.

This invention belongs to the field of food biotechnology and bioactive peptides, and specifically relates to a cottonseed protein-derived ACE-inhibiting peptide, its screening, modification methods, and applications. The screening method of this application includes four core steps: virtual enzymatic library construction, multi-stage computer filtering, molecular docking screening, and in vitro activity verification, obtaining 194 high-confidence candidate peptides. Four cottonseed protein-derived peptides (SYF, DIF, IAF, and RFY) with in vitro ACE-inhibiting activity were verified, all exhibiting mixed ACE-inhibiting activity, with DIF showing the strongest inhibitory activity. Molecular dynamics simulations confirmed the structural stability of the ACE-DIF complex. A modified peptide FIF was obtained through a dipeptide module substitution strategy, with an IC50 value of [missing information]. 50 The activity was significantly reduced, approximately 3.7 times that of the parent peptide DIF, and the inhibition type changed from mixed to competitive. This invention provides a new approach for the development of natural antihypertensive functional peptides and the high-value utilization of plant protein resources.

The application discloses a pentapeptide ES5 with ACE inhibitory activity and a preparation method and application thereof, and belongs to the technical field of biotechnology.The amino acid sequence of the pentapeptide ES5 is ERQFS, and the pentapeptide ES5 can be prepared by a solid-phase synthesis method and an enzymatic hydrolysis method.The pentapeptide ES5 is identified from a protease hydrolysate of red algae (real Gracilaria) agar residue, has potential interaction with ACE, and has an ACE inhibition rate of 90.84% at a concentration of 0.1 mg / mL; compared with VPP and IPP (the ACE inhibition rates are 82.32% and 78.00% respectively at the same concentration), the ACE inhibitory activity is extremely significantly increased (p<0.001); and the pentapeptide ES5 can be used for preparing antihypertensive drugs or antihypertensive functional food.

The present application relates to a kind of preparation method and application of egg yolk polypeptide with ACE inhibitory activity, the method comprises: Q1.degreased egg yolk powder is dissolved in appropriate amount of water, adjust pH to 6~8;Q2.50~60 ℃, pH=7‑8 under the condition of adding alkaline protease or flavour protease is carried out enzymolysis 1.5~2.5h, then enzyme is killed, and the egg yolk hydrolysate is obtained.The present application not only uses step-by-step enzymatic hydrolysis to prepare high-activity ACE inhibitor, and using the method of bioinformatics, 5 ACE inhibitory peptides (KFIPL, KFLPGY, KFLPTF, ATPFGKL, KLPDMILY) with high activity are screened and identified, and in order to solve the problem of polypeptide instability after sterilization in functional beverage, CMC and GG are used as stabilizer, and the problem of enzymatic polypeptide in functional beverage is solved.

The invention discloses an active peptide with antihypertensive activity and a preparation method and application thereof, and relates to the technical field of dairy products. A novel active peptide is obtained through screening, and the amino acid sequence of the novel active peptide is LDAYPGSAW. Through in-vitro activity evaluation, the polypeptide not only has relatively strong ACE inhibitory activity, but also has relatively strong oxidation resistance, the IC50 value for inhibiting ACE is 62.37 [mu] M, and the TEAC value for scavenging free radicals is 2.10 [mu] mol TE / [mu] mol. In-vivo blood pressure reduction evaluation is carried out through a spontaneous hypertension rat model, and it is shown that the compound also has an excellent blood pressure reduction effect in vivo and can be applied to preparation of products related to blood pressure reduction and / or oxidation resistance.

The invention relates to the technical field of food and / or medicine, and provides a cicada pupa small-molecule active peptide and a preparation method and application thereof.The preparation method comprises the steps that cicada pupa serves as a raw material, and the small-molecule active peptide is prepared through extraction, enzymolysis, macroporous resin separation and sephadex separation; the obtained small molecule active peptide has remarkable ACE inhibitory activity and good antioxidant activity, and shows huge application potential in the fields of drug development, health care product development, functional food addition and the like.

The invention discloses lactobacillus helveticus IMAU16360 with ACE (Angiotensin Converting Enzyme) inhibitory activity and application of the lactobacillus helveticus IMAU16360 in fermented milk, and belongs to the field of microorganisms. The lactobacillus helveticus IMAU16360 is preserved in the China General Microbiological Culture Collection Center (CGMCC), the preservation number is CGMCC No.36567, the preservation time is November 11, 2025, and the preservation address is No.3, Yard 1, Beichen West Road, Chaoyang District, Beijing. The commercial leavening agent PYS-010 and the lactobacillus helveticus IMAU16360 are compounded according to the ratio of 1: 1, the pH and acidity change trend of the compounded group in the storage period is consistent with that of a control group, the compounded group is fused into an existing process system, and an existing production line does not need to be replaced; the elasticity and viscosity of a single strain fermented product are improved, so that the product is fuller in taste; on the premise that the basic taste is not influenced, the fermented milk has ACE inhibitory activity; meanwhile, a relatively high level of viable count is maintained, and good market value is achieved.

The invention relates to the technical field of activity detection, and particularly discloses a colloidal gold method for rapidly detecting ACE inhibitory activity and a test paper preparation method. The test paper comprises a sample pad, a gold-labeled pad, a nitrocellulose membrane and a water absorption pad, angiotensin converting enzyme labeled by colloidal gold is loaded on the gold-labeled pad, and a detection line coated with an angiotensin II antibody and a quality control line coated with an anti-ACE antibody are arranged on the nitrocellulose membrane. During detection, an ACE inhibitor in a sample can inhibit enzyme activity and influence aggregation of colloidal gold on the detection line, so that rapid judgment is realized through color comparison of the detection line and the quality control line. The invention also provides a preparation method and a detection method of the test paper, and the test paper has the advantages of simplicity and rapidness in operation, no need of large instruments, low cost, suitability for field detection and the like, and can be widely applied to drug screening, food detection and clinical sample preliminary screening.

The invention belongs to the technical field of bioactive components, and discloses a high-activity oat peptide as well as a preparation method and application thereof. The preparation method comprises the following steps: crushing the oat bran, adding water, carrying out primary enzymolysis with cellulase and xylanase, treating with L-cysteine, and carrying out ultrasonic extraction to obtain a crude extract; carrying out ammonium sulfate salting-out on the crude extract to obtain a reconstitution fluid, and treating with a composite adsorbent to obtain an enriched fluid; adding compound protease into the enriched liquid for secondary enzymolysis (containing a protective agent and inert gas) to obtain enzymatic hydrolysate; performing microfiltration and ultrafiltration on the enzymatic hydrolysate, adding glycine and glutamine transaminase for third enzymolysis, and concentrating and drying to obtain a finished product. The prepared oat peptide is high in protein dissolution rate and active peptide retention rate, excellent in ACE inhibitory activity and alpha-glucosidase inhibitory activity and good in storage stability, and can be used for preparing products for reducing blood pressure and blood sugar.

The invention relates to the technical field of microbial medicines, and discloses application of lactobacillus plantarum in preparation of products for relieving hypertension and related complications. The lactobacillus plantarum is classified and named as Lactiplantarum P517, the lactobacillus plantarum is preserved in the China Center for Type Culture Collection, the preservation number is CCTCC NO: M 20252435, and the 16S rDNA gene sequence of the lactobacillus plantarum is as shown in SEQ ID NO: 1. The strain is separated from feces of healthy adults, and the lactobacillus plantarum provided by the invention is gram-positive bacteria, has high ACE inhibitory activity, better oxidation resistance, better acid resistance and cholate resistance, and also has excellent automatic agglutination capability and antibacterial performance; the method can relieve hypertension and related complications (intestinal injury, kidney injury and heart injury) caused by high-salt diet to a certain extent, can be widely applied to production of food, health care products, medicines and the like, and has a wide prospect.

The application discloses a method for screening multifunctional peptides of perilla albumin source for treating diabetes and hypertension, which comprises the following steps: obtaining perilla albumin sequence, predicting the potential of perilla albumin as DPP-Ⅳ and ACE inhibitory peptide source, performing computer simulation digestion, and screening DPP-Ⅳ and ACE inhibitory peptides. Through the bioactive peptide database BIOPEP-UWM, 40 perilla albumin sequences are predicted for active fragments, and it is found that perilla albumin is rich in a large number of fragments with DPP-Ⅳ and ACE inhibitory activity. Perilla albumin is subjected to computer simulation digestion, polypeptides capable of resisting gastrointestinal digestion are obtained, and perilla albumin source multifunctional peptides with DPP-Ⅳ and ACE inhibitory activity are screened through related activity prediction and molecular docking. The practicability of the multifunctional peptides is predicted, and two multifunctional peptides without toxicity, allergenicity, bitterness and hemolytic activity, namely CDAF and CCAL, are screened. The two multifunctional peptides inhibit the catalytic activity of DPP-Ⅳ and ACE by competitively combining with the active centers of DPP-Ⅳ and ACE, so as to play the roles of reducing blood sugar and blood pressure.

An ACE inhibitory peptide based on A2 beta-casein and a preparation method and application thereof belong to the technical field of bioactive peptides. To solve the technical problem that ACE inhibitor drugs in the prior art have many adverse reactions and are not suitable for long-term continuous use, a preparation method of an ACE inhibitory peptide based on A2 beta-casein is provided. The A2 beta-casein is subjected to enzymolysis by pepsin and trypsin, and through ultrafiltration, desalting, liquid chromatography tandem mass spectrometer analysis and molecular docking analysis, an ACE inhibitory peptide with an amino acid sequence shown in SEQ ID No. 1 is finally screened and obtained. The ACE inhibitory peptide can effectively increase the release amount of NO of human umbilical vein endothelial cells and significantly inhibit the secretion of ET1, and has no toxic side effects on human umbilical vein endothelial cells. The ACE inhibitory peptide provided by the application can be applied to the preparation of a drug for preventing or reducing blood pressure.

The invention discloses a pentapeptide RA5 with ACE inhibitory activity and a preparation method and application thereof, and belongs to the technical field of small molecule peptides. The amino acid sequence of the pentapeptide RA5 is RVVVA, and the pentapeptide RA5 can be prepared through a solid-phase synthesis method and an enzymolysis method. The pentapeptide RA5 is identified from eucheuma proteolysis liquid and has potential interaction with ACE, under the concentration of 0.1 mg / mL, the ACE inhibition rate is 86.88%, and compared with VPP and IPP (under the same concentration, the ACE inhibition rates are 82.32% and 78.00% respectively), the ACE inhibition rates are remarkably increased (plt; 0.01) and an extremely significantly increased (plt; the pentapeptide RA5 has a higher blood pressure lowering function than VPP and IPP, and can be used for preparing blood pressure lowering drugs or functional foods assisting in lowering blood pressure.

The invention discloses an angiotensin converting enzyme inhibitory peptide screening method and system based on machine learning, and belongs to the technical field of bioinformatics. The angiotensin converting enzyme inhibitory peptide screening method based on machine learning comprises the following steps: step 1, constructing a peptide sequence data set, wherein the data set comprises known ACE inhibitory peptides and non-inhibitory peptides; 2, extracting multi-dimensional features from the peptide sequence, wherein the multi-dimensional features comprise physicochemical property features and sequence composition features; 3, training and optimizing a machine learning model by using the multi-dimensional features to obtain an ACE inhibitory peptide prediction model; and 4, performing activity prediction and virtual screening on a to-be-screened peptide sequence by using the ACE inhibitory peptide prediction model to obtain candidate ACE inhibitory peptides. According to the method, the multi-dimensional physicochemical and sequence characteristics of the peptide sequence are extracted, and the XGBoost machine learning prediction model is constructed and optimized, so that high-throughput virtual screening of 2-5 peptides can be realized, and the high-activity ultra-short ACE inhibitory peptide is efficiently explored.

This invention discloses an ACE inhibitory peptide YVLL derivative and its application, belonging to the field of peptide derivative technology. The ACE inhibitory peptide YVLL derivative has the structural formula shown in Formula 1 or Formula 2. The ACE inhibitory peptide YVLL derivative and its application of this invention significantly enhance the ACE inhibitory activity of the acetylated and formylated derivatives of the ACE inhibitory peptide YVLL, increasing by 394.7% and 373.8% respectively compared to the ACE inhibitory peptide YVLL. The antihypertensive effect is also significantly enhanced, with the maximum antihypertensive value increasing by 145.2% and 82.4% respectively compared to the ACE inhibitory peptide YVLL.

The present application relates to the field of functional food and medicine, and particularly relates to a polypeptide with blood pressure lowering function and a preparation method thereof. The amino acid sequence of the polypeptide is as shown in any one of the following: 1) Leu-Ser-Leu; 2) Ile-Ser-Leu; 3) Leu-Ser-Ile; or 4) Ile-Ser-Ile. The polypeptide has excellent ACE inhibitory activity, good digestive stability, and can be obtained from natural edible materials, thus further ensuring safety. Therefore, the functional polypeptide can be used in health products for patients with hypertension and as a lead drug for blood pressure lowering drugs.

This invention relates to the field of bioactive peptide technology and discloses a method for the targeted preparation of ACE inhibitory peptides from donkey-hide gelatin. The steps are as follows: (1) pretreatment of donkey-hide gelatin; (2) enzymatic hydrolysis; (3) peptidomics analysis; (4) bulk molecular docking of donkey-hide gelatin peptides with ACE functional receptors; (5) membrane separation and targeted enrichment; (6) the enriched flavor peptides are concentrated by rotary evaporation, then a cryoprotectant is added, and the mixture is freeze-dried under vacuum to obtain freeze-dried donkey-hide gelatin peptide powder. The product prepared by this invention has a rich sweet taste and a subtle umami flavor, accompanied by a slight meaty aroma, plant aroma and nutty aroma. It has a mellow taste, a smooth mouthfeel, and almost no obvious bitterness or astringency.

The application discloses an enzymatic hydrolysate of red date protein, which contains ACE inhibitory peptides and DPPIV inhibitory peptides; the amino acid sequence of the ACE inhibitory peptides is Arg-Leu-Pro-His-Val or Thr-Val-Lys-Pro-Gly-Leu or Lys-Ala-Leu-Val-Ala-Pro; and the amino acid sequence of the DPPIV inhibitory peptides is Tyr-Leu-His-Leu or Ile-Pro-Ile-Tyr or Lys-Ala-Leu-Val-Ala-Pro. The application further discloses a preparation method of the enzymatic hydrolysate of red date protein and application thereof. The proteinase is added in the red date protein at 0.3-2% (w / w) in terms of mass percentage, and enzymolysis is carried out at 37 DEG C for 2-3 hours, and then the enzymatic hydrolysate of red date protein is obtained through freeze-drying; or the enzymatic hydrolysate of red date protein is obtained through enzymolysis of the red date protein in the gastrointestinal tract. The enzymatic hydrolysate of red date protein has the effects of assisting in reducing blood pressure and reducing blood sugar, and can be used for preparing functional food or health-care food with the functions of assisting in reducing blood pressure and reducing blood sugar, and is particularly suitable for patients with diabetes accompanied by high blood pressure, and has a good market application prospect.

The present application belongs to the field of bioactive peptides. The present application provides an angiotensin converting enzyme inhibitory peptide and a screening method and application thereof, and the amino acid sequence of the inhibitory peptide is GKGLW and / or GDGLKW. The screening method of the inhibitory peptide of the present application uses soybean protein as raw material, adopts targeted alkaline protease and chymotrypsin double enzymolysis technology for preparation and screening, realizes rapid screening of ACE inhibitory peptides, and provides experimental basis for structure-activity relationship. The inhibitory peptide has strong blood pressure lowering activity, high safety and no toxic side effects. The determination of the stability of the digestive tract shows that the inhibitory peptide has good gastrointestinal stability in the gastrointestinal digestion, and can still maintain good activity after entering the blood.

The application provides a scomber ACE inhibitory peptide as well as a preparation method and application thereof, and relates to the technical field of ACE inhibitory peptides.The scomber ACE inhibitory peptide provided by the application comprises an amino acid sequence as shown in SEQ ID NO.1 or SEQ ID NO.2.Through papain enzymolysis, ultrafiltration, gel chromatography, liquid chromatography-tandem mass spectrometry sequence identification and QSAR modeling, the application improves the working efficiency of ACE inhibitory peptide screening, prepares and screens scomber ACE inhibitory peptides with high angiotensin converting enzyme inhibitory effect, and the scomber ACE inhibitory peptides can be used for developing new antihypertensive drugs, and have a wide application prospect.

This invention discloses a pentapeptide RA5 with ACE inhibitory activity, its preparation method, and its applications, belonging to the field of small molecule peptide technology. The amino acid sequence of the pentapeptide RA5 is RVVVA, and it can be prepared by solid-phase synthesis and enzymatic hydrolysis. The pentapeptide RA5 was identified from the protein hydrolysate of *Euphorbia milii* and exhibits potential interaction with ACE. At a concentration of 0.1 mg / mL, the ACE inhibition rate is 86.88%, which is significantly increased (p<0.01) and extremely significantly increased (p<0.001) compared to VPP and IPP (at the same concentration, the ACE inhibition rates are 82.32% and 78.00%, respectively). The pentapeptide RA5 has a stronger antihypertensive function than VPP and IPP and can be used to prepare antihypertensive drugs or functional foods that assist in lowering blood pressure.

This invention discloses an ACE-inhibiting peptide, its screening method, and its applications, relating to the field of food and beverage technology. The active peptide with a specific amino acid sequence exhibits high ACE-inhibiting activity, with an IC50 value of [missing information]. 50 The concentration ranges from 0.31 to 1.93 mg / mL. This bioactive peptide exhibits no toxic side effects at the cellular level and is characterized by its small molecular weight, safety, stability, and easy absorption. It can be used to prepare ACE inhibitory drugs for the prevention or treatment of hypertension, or as a functional food additive to prepare supplementary blood pressure-lowering health foods for long-term health maintenance by hypertensive patients.

This invention discloses ACE inhibitory peptide TLRFALHGME derivatives and their applications, belonging to the field of peptide derivative technology. The structural formulas are shown in Formula 1 or Formula 2: Formula 1, Formula 2. The ACE inhibitory peptide TLRFALHGME derivatives and their applications of this invention significantly enhance the antihypertensive effect of acetylated and myristylated derivatives of ACE inhibitory peptide TLRFALHGME, with maximum antihypertensive values ​​increased by 160.7% and 125.7% respectively compared to ACE inhibitory peptide TLRFALHGME.

The invention discloses a potato protein ACE (angiotensin converting enzyme) inhibitory peptide as well as a preparation method and application thereof, and relates to the technical field of medicines, health-care products and foods. The preparation method of the potato protein ACE inhibitory peptide comprises the following steps: adding alkaline protease into potato protein powder for enzymolysis, adding a trichloroacetic acid solution into enzymatic hydrolysate, heating, centrifuging, collecting supernate, and freeze-drying to obtain the potato protein ACE inhibitory peptide. The invention also provides a potato protein ACE inhibitory peptide solid beverage. The potato protein ACE inhibitory peptide has the effect of reducing blood pressure, and a new thought is provided for relieving or treating hypertensive patients. The potato protein ACE inhibitory peptide powder in the solid beverage disclosed by the invention is subjected to secondary extraction by utilizing waste potato dregs generated by potato processing products, so that comprehensive utilization and green, sustainable and healthy development are realized, and the cost of raw materials is saved.

This invention discloses a turtle peptide with angiotensin-converting enzyme (ACE) inhibitory activity, its preparation, and its application, belonging to the field of biotechnology. Using turtle as raw material, this invention obtains peptide components with a molecular weight less than 5 kDa through enzymatic hydrolysis and ultrafiltration. Sequencing reveals three peptide sequences with ACE inhibitory activity: AAGPAGGNLN, EAGAQGPPGA, and GARGDK. In vitro activity experiments verified the ACE inhibitory function of the three turtle peptides. The turtle peptide combination prepared by this invention exhibits significant ACE inhibitory activity and can be used to develop functional foods or antihypertensive drugs. Furthermore, the preparation method is environmentally friendly, efficient, and suitable for industrial production.