282 results about "Ace inhibitory" patented technology

The present invention relates generally to functional sweetener compositions comprising non-caloric or low-caloric natural and/or synthetic high-potency sweeteners and methods for making and using them. In particular, the present invention relates to different functional sweetener compositions comprising at least one non-caloric or low-caloric natural and/or synthetic high potency sweetener, at least one sweet taste improving composition, and at least one functional ingredient, such as rubisco protein, rubiscolin, rubiscolin derivatives, ACE inhibitory peptide, and combinations thereof. The present invention also relates to functional sweetener compositions and methods that can improve the tastes of non-caloric or low-caloric high-potency sweeteners by imparting a more sugar-like taste or characteristic. In particular, the functional sweetener compositions and methods provide a more sugar-like temporal profile, including sweetness onset and sweetness linger, and/or a more sugar-like flavor profile.

The invention discloses a novel orally-effective spirulina source antihypertensive peptide and a preparation method thereof. The antihypertensive peptide is a tripeptide with the sequence of isoleucine-glutamine-proline (Ile-Gln-Pro, IQP). An angiotensin converting enzyme (ACE) inhibitory peptide is prepared by the following steps of: performing enzymolysis on spirulina platensis by adopting alkali protease, and performing ultrafiltration, gel filtration chromatography and secondary reversed phase high-performance liquid chromatography purification. Tests prove that the IQP is a noncompetitive inhibitor of ACE, and can keep good ACE inhibitory activity under digestion of main gastrointestinal protease. The IQP has good antihypertensive effect on essential hypertension rat (SHR) and has great application prospects in medicaments and functional foods for preventing and treating hypertension.

The invention relates to development and utilization of maize polypeptide products and discloses a method for preparing maize polypeptide through synchronous multi-frequency ultrasonic-aided enzymatic hydrolysis by immobilized enzyme, belonging to the technical field of deep processing of maize. The method comprises (1) a preparation process for sodium alginate-immobilized alkali protease; and (2) a process for synchronous multi-frequency ultrasonic-aided enzymatic hydrolysis of zein by immobilized enzyme. According to the invention, synchronous multi-frequency ultrasonic wave and immobilized enzyme combined technology is employed for enzymatic hydrolysis and preparation of the polypeptide product, so the method provided by the invention has the following advantages compared with conventional methods utilizing enzymatic hydrolysis, single-frequency ultrasonic-aided enzymatic hydrolysis and double-frequency ultrasonic-aided enzymatic hydrolysis: the prepared maize polypeptide has higher ACE inhibitory activity, and the yield and activity of the polypeptide are better improved. The immobilized enzyme is recoverable; the repeated utilization rate of the enzyme is higher; production cost is better saved; and a feasible approach is provided for industrial production of a high-added-value maize polypeptide product.

The invention discloses a method for extracting silk peptide from cocoon fiber, which belongs to the technical field of preparation of the silk peptide. The method comprises the following steps: the cocoon fiber is added into a Na2CO3 solution for refining, filtrated and added into a CaCl2 solution for dissolution; protease for silk dissolution solution obtained after dissolution undergoes enzymatic hydrolysis reaction; after ultrafiltraiotn and nano-filtration separation of digest, a feed liquid is obtained; and the silk peptide powder is obtained after spray drying of the feed liquid. The method has the advantages that ACE inhibitory polypeptide is enriched through ultrafiltraiotn and nano-filtration separation of the digest; the efficiency of removing CaCl2 salt is high; the method has the advantages of large processing capacity, mild separation condition, simple flow, low energy consumption and so on; and the industrial production is easy to realize.

The invention discloses a preparation method of rice bran protein peptide with ACE inhibitory activity. The method is characterized in that rice bran is adopted as a raw material, and comprises the following steps: firstly extracting rice bran protein, then preparing rice bran protein hydrolysate by adopting an enzyme hydrolysis method, and separating and purifying the rice bran ACE inhibitory peptide by adopting a hyperfiltration method, sephadex G-15 and a RP-HPLC method. According to the preparation method, the rich and cheap rice bran resource is effectively utilized to prepare the rice bran protein peptide with the high ACE inhibitory activity. The decompression function of the rice bran ACE inhibitory peptide can be maximally utilized, so that on one hand, the additional value of the rice bran is increased, and the good economic benefit and market value can be created; on the other hand, the morbidity of the hypertension can be effectively reduced, the high morbidity can be controlled, the human beings can be protected from the harm of the hypertension, the application value and social benefit are good, and the theoretical foundation and the experiment evidence can be provided for researching the structure and decompression mechanism of the rice bran protein ACE inhibitory peptide.

Milk ACE inhibiting peptide is prepared by: hydrolyzing milk casein as raw material with protease, heating the hydrolytic liquid to inactivate protease, centrifugally separating to obtain supernatant, desalting with ion exchange resin, freezing to dry and obtain high active edible ACE inhibiting peptide. The product can be used as food additive as functional factor or as food supplementary to add into various foods.

The invention relates to a lactobacillus and in particular relates to a low-temperature resistant lactobacillus with ACE (angiotensin converting enzyme) inhibitory activity. The low-temperature resistant lactobacillus with the ACE inhibitory activity is lactobacillus plantarum I1 which is preserved in the China general microbiological culture collection center of which the preservation address is No.3, No1. yard, Beichen west road, Chaoyang District of Beijing, the preservation data is September 18th, 2012, and the preservation number is CGMCC No.6575; the lactobacillus plantarum I1 has the ACE inhibitory rate being 66%, can tolerate 6% of NaCl and 100mg/kg of nitrite, and can fast generate acid; the growing temperature of the lactobacillus plantarum I1 is 4-40 DEG C, and is suitable for low-temperature fermentation; and the low-temperature resistant lactobacillus has obvious protease activity and lipase activity, can not generate mucus, has an obvious antibacterial effect on staphylococcus aureus and colon bacillus, and can be used as a meat product leavening agent strain under a low-temperature condition.

The invention discloses walnut peptide having the ACE inhibitory activity and a preparation method thereof. The amino acid sequence of the walnut peptide is EPNGLLLPGY, and the preparation method of the walnut peptide comprises the steps of taking walnut pomace as raw materials, selecting appropriate protease, preparing walnut antihypertensive peptide coarse products according to the optimal enzymolysis technology, adopting the coupling chromatographic technique of the membrane technology for conducting fine preparation on the walnut antihypertensive peptide, adopting the high performance liquid chromatography for conducting further purification, and adopting the vacuum freeze drying or low-temperature spray drying technology for preparing final products. According to the walnut peptide, the walnut pomace is adopted as the raw materials for developing the protein peptide, the raw materials are abundant, the price is low, and the large-scale industrialized production can be satisfied; the walnut peptide has important significance in walnut protein product diversification and development and utlization of walnut biological active peptide in functional foodstuff, health food or medicine.

The invention provides a method for preparing ACE (Angiotensin Converting Enzyme) inhibitory peptides through bean pulp enzymolysis. The method comprises the following steps that (1) high-temperature bean pulp is crushed and sieved, obtained bean pulp powder and water are mixed according to a mass ratio of 1:(4-6), the pH value is regulated to 8 to 10, and a bean pulp mixture is obtained; (2) the pH value of the bean pulp mixture is regulated to 7 to 9, a complex enzyme preparation is added, the mixture is uniformly mixed, hydrolysis is carried out for 5 to 8 hours at the temperature being 35 to 50 DEG C, after the hydrolysis is completed, hydrolysis liquid is boiled in a boiling water bath for enzyme deactivation, and mixed liquid is obtained; (3) the pH value of the mixed liquid is regulated to 6.5 to 7.5, supernatant is taken, ultrafiltration treatment is performed through an ultrafiltration membrane, and filtered liquid is obtained; and (4) the filtered liquid is subjected to vacuum freezing drying, and the ACE inhibitory peptide powder is obtained. The method for preparing ACE inhibitory peptides through bean pulp enzymolysis has the advantages that the process is simple and is easy to implement; in the whole process, the reaction conditions are mild; the compounded complex enzyme preparation is used; and the industrial processing operation is convenient.

Improved processes are provided for preparing ACE inhibitory peptide containing hydrolysates from a plant material such as a seed meal or flour. In one embodiment, the seed meal or flour is extracted with an organic solvent prior to digestion. Also provided are ACE inhibitory peptides Val-Ser-Val and Phe-Leu.

The invention discloses an ACE inhibitory peptide and a preparation method thereof, and provides a casein hydrolysate which has an anti-hypertension effect and is rich in the ACE inhibitory peptide, the ACE inhibitory peptide with an anti-hypertension effect, and a preparation method of the ACE inhibitory peptide. The casein hydrolysate rich in the ACE inhibitory peptide contains two peptide fragments with ACE inhibitory activities; and the amino acid sequences of the two peptide fragments with ACE inhibitory activities are respectively YS and YQKFPQYLQY. The amino acid sequence of the ACE inhibitory peptide is YS or YQKFPQYLQY. The ACE inhibitory peptide or the casein hydrolysate rich in the ACE inhibitory peptide is sufficiently mixed with milk powder matrixes to prepare anti-hypertension milk powder; or the ACE inhibitory peptide or the casein hydrolysate rich in the ACE inhibitory peptide is mixed with fresh milk to obtain a mixture, and lactic acid bacteria is inoculated in the mixture to obtain yoghourt with an anti-hypertension effect. The dairy product has a remarkable anti-hypertension effect, has no influence on the normal blood pressure, is safe to eat, and is suitable for being ate by the hypertension groups.

The invention mainly relates to Larimichthys crocea connetin source ACE inhibitory peptide sequences and applications thereof. The two kinds of peptide sequences respectively are WAR and WQR, and establish a basis for prevention and treatment of hypertension. Different on-line databases are used, and after multiple times of screening, the inhibitory peptides with high efficient ACE inhibition activity are obtained. WAR and WQR are artificially synthesized, in order to verify external ACE inhibition activity of the peptides by using a high performance liquid chromatography method. The inventionrelates to two kinds of active peptides with good external ACE inhibition activity. The two kinds of peptide sequences are used for developing health food with blood pressure adjusting effects, and the purpose for prevention, control, alleviation and auxiliary treatment of hypertension is realized by long-term usage.

The invention provides green tea goat milk with a function of reducing blood pressure and a preparation method of the green tea goat milk. Goat milk beverage which is rich in ACE inhibitory peptides is prepared by compound enzymolysis, is low in production cost, not limited by resource and small in environmental pollution, and can be mass-produced, and products are safe. On the basis, ultra-fine green tea powder is prepared by enzymic hydrolysis of cell walls and a low-temperature ultra-fine pulverizing technology, specific surface area is increased, and a large number of effective constituents are released. The ultra-fine green tea powder is added in the goat milk in proportion, and the obtained product not only has various nutrient substances and tea fragrance in green tea, but also has treatment effect of reducing high blood pressure, and has wide application prospect in the food industry. The tea powder is prepared by low-temperature ultra-fine pulverization, the original color, flavor and taste quality of tea leaves can be maintained effectively, after the tea leaves is pulverized into ultra-fine powder, the specific surface area of the tea powder is increased obviously, the effective constituents are released easily, human absorption can be improved, and therefore, nutrition absorptivity of human to the tea leaves is improved. Ultra-fine pulverization is a pure physical process, and pure natural property of processed products is maintained.

The invention discloses a method for preparing angiotensin-I-converting enzyme (ACE) inhibitory peptide by using enzymolysis goat milk casein. The method comprises the following steps of: centrifugally degreasing recovered goat milk to obtain skimmed milk; preparing casein by using an isoelectric precipitation method; freezing and drying casein precipitate; dissolving the obtained casein freeze-dried powder to prepare a casein solution; performing hydrolysis on the casein solution by adding different kinds of enzymes; and optimizing the hydrolysis condition and treating a hydrolysis process in an ultrasonic wave mode, so that catalytic activity is improved to ensure that the hydrolysis reaction is complete; and because of the obtained micromolecule ACE inhibitory peptide, obvious pressure reduction function on hypertensive can be achieved, the influence on normal blood pressure is avoided, and the method has the characteristics of no toxic and side effects, high security and easiness for absorption.

The invention discloses a method for preparing a silkworm chrysalis source ACE (Angiotensin Converting Enzyme) inhibitory peptide from by an ultrasonic-ionic liquid coupling technology. The method comprises the following steps: preparing silkworm chrysalis protein into a suspension by using distilled water, subsequently mixing the suspension with an isopyknic ionic liquid and adding into a flask, dipping an ultrasonic probe into a water phase so as to perform ultrasonic-ionic liquid coupling treatment, after the treatment is accomplished, centrifuging, collecting the silkworm chrysalis protein and recycling the ionic liquid, firstly washing the collected silkworm chrysalis protein and subsequently centrifuging so as to remove the residual ionic liquid, and preparing the ACE inhibitory peptide from pretreated silkworm chrysalis protein by using an enzyme method. Compared with the conventional untreated method, the method for preparing the silkworm chrysalis source ACE inhibitory peptide by an ultrasonic-ionic liquid coupling technology remarkably improves the activity of the ACE inhibitory peptide, that is, the activity is increased by 34-54%.

The invention relates to a method for preparing an angiotensin converting enzyme (ACE) inhibitory peptide from sea cucumber. The method comprises the following steps of: de-salting and softening raw materials including fresh sea cucumber, or dry sea cucumber, or soaked sea cucumber, or high-pressure sea cucumber; after preparing slurry, adding water into the slurry; heating the slurry to kill microorganisms; after cooling the slurry, adding composite proteolytic enzyme or flavor proteolytic enzyme into the slurry to perform enzymolysis; killing enzymes; after cooling, centrifuging the product to remove undissolved substances; filtering the supernatant fluid in a pipe; filtering the filtrate with a 3,000 Dalton organic membrane to trap and classify; and performing nano filtration and drying on the trapped liquid which is less than 3,000 Dalton in mass to obtain the ACE inhibitory peptide. A zymolytic technical scheme is optimized; and due to the separation and extraction technology of pipe filtration, ultrafiltration and nano filtration, the extraction efficiency is high, and the pressure-reducing effect of the obtained ACE inhibitory peptide is good. The method is reasonable in process, simple and convenient in operation and high in operability; and industrial production is easy to realize.

The invention belongs to the field of deep processing of edible protein and comprehensive utilization of by-products thereof and discloses a method for preparing egg white source ACE inhibitory peptide microcapsules. The method is characterized by comprising the following steps: with egg white protein powder with protein content of 80-90% as a raw material, carrying out enzymolysis, ultrafiltration, vacuum freeze drying and microcapsule embedding process of spray condensation technology to prepare the egg white source ACE inhibitory peptide microcapsules. The embedding rate and ACE inhibition ratio of a microcapsule product obtained according to the preparation method provided by the invention are higher than 80%, the microcapsule product is natural and safe and is good in palatability and stability, and moreover, the peptide digestion is not affected. Therefore, the technology for preparing the egg white source ACE inhibitory peptide microcapsules provided by the invention provides an efficient and new way for deep processing and comprehensive utilization of egg products.

The invention relates to a method for preparing angiotensin I-converting enzyme (ACE) inhibitory peptide by using a squid liver protein, which comprises: treating squid liver protein from which fish oil is extracted with acetone to degrease; hydrolyzing the squid liver protein in the presence of pepsase for 18 to 25 hours under conditions that: the hydrolysis temperature is 35 to 38 DEG C, the pH is 1.5 to 2.5, the ratio of the pepsase to a substrate is 2 to 3 percent, and the concentration of the substrate is 1 to 3 percent; and subjecting enzymolysis solution to ultrafiltration treatment, tracking the ACE inhibiting activity of the ACE inhibitory peptide, and separating and purifying the ACE inhibitory peptide by using gel filtration chromatography, diethylaminoethanol (DEAE) anion exchange, reversed-phase-high performance liquid chromatography (RP-HPLC) and other methods. The ACE inhibiting activity of the prepared ACE inhibitory peptide is basically unchanged at a temperature of 2 to 100 DEG C and a pH value of 1 to 12 and is stable; and in a simulation gastrointestinal digestion experiment, the ACE inhibiting activity of the components is not affected, and the peptide still has high ACE inhibiting activity after being digested in gastrointestinal tract. The method is reasonable in process, simple in operation, reduces waste discharge, changes waste materials into valuable materials, and the prepared ACE inhibitory peptide can be used in the field of development of blood pressure reducing foods and medicines.

The invention discloses an ACE (angiotension converting enzyme) inhibitory peptide and a preparation method thereof, and provides an ACE inhibitory peptide with an anti-hypertension effect and a preparation method of the ACE inhibitory peptide. An amino acid sequence of the ACE inhibitory peptide is YS. The preparation method comprises the following steps: sufficiently mixing the ACE inhibitory peptide with a milk powder substrate to prepare anti-hypertension milk powder; or mixing the ACE inhibitory peptide with fresh milk, and inoculating lactic acid bacteria to ferment to obtain yoghourt with the anti-hypertension effect. The milk product has a remarkable antihypertensive effect, is free of interferences on normal blood pressure, safe to eat, and suitable for the hypertension crowds.

The invention discloses a puffer fish bioactive peptide and a preparation method thereof and belongs to the technical field of ACE (Angiotensin Converting Enzyme) inhibitory peptides. The preparationmethod of a puffer fish bioactive peptide comprises the following steps: S1, preparation of a puffer fish enzymatic hydrolysate; S2, ultrafiltration separation of the puffer fish enzymatic hydrolysate; S3, separation and purification of polypeptide; S4, sequence identification of polypeptide; and S5, polypeptide synthesis and activity determination. According to the puffer fish bioactive peptide,the bioactive peptide is octapeptide, and the amino acid sequence of the puffer fish bioactive peptide is PPLLFAAL (Pro-Pro-Leu-Leu-Phe-Ala-Ala-Leu). According to the puffer fish bioactive peptide andthe preparation method thereof, the puffer fish skin is adopted as a raw material, an octapeptide amino acid sequence is obtained, and the prepared polypeptide has the ACE inhibitory activity.

The invention provides a mung bean yoghourt product having the function of reducing the blood pressure and a preparation method thereof. The mung bean yoghourt product is prepared from mung bean extract and milk powder, wherein the mung bean extract is prepared by soaking mung beans in a glutamic acid solution; and the mung bean extract accounts for 80 to 90 weight percent, and the milk powder accounts for 10 to 20 weight percent. The preparation method comprises: soaking the mung beans in the glutamic acid solution, accelerating germination of the mung beans, and performing seed coat removal,homogenizing and high-temperature amylase treatment to obtain the mung bean extract; adding skim milk powder into the mung bean extract, performing mixing and homogenization treatment, adding protease for enzymolysis, and obtaining a fermentation substrate; and performing enzyme killing, sterilization, cooling and lactobacillus inoculation on the fermentation substrate, and performing fermentation on the fermentation substrate to prepare the mung bean yoghourt product. The mung bean yoghourt product not only is rich in GABA but also contains ACE inhibitory peptide, has good effect of reducingthe blood pressure, not only can be directly used for preparing related health food but also can be used as a functional composition and added into other health products, and has wide application prospect.

The invention relates to a technological method for co-production of ACE inhibitory peptides and immunological competence peptides of mung beans with an enzymic method. The technological method for co-production of the ACE inhibitory peptides and the immunological competence peptides of the mung beans with the enzymic method comprises the steps that mung bean albumen powder is dissolved in micromolecule water and is prepared into an albumen solution with the concentration, by mass, ranging from 7% to 40%, enzymolysis and inactivation are conducted on mung bean albumen, and supernate is moved out; mung bean antihypertensive peptides are prepared, wherein mung bean peptides with the molecular weight of 1 kDa and 3 kDa respectively are processed through an ultrafiltration membrane, and an ACE inhibitory peptide powder product of the mung beans is obtained by conducting freezing drying on the 1 kDa-3 kDa filtered solution obtained after interception is conducted; the immunological competence peptides of the mung beans are prepared, wherein the residual solution is hydrolyzed continually for 2-3 hours through neutral protease and flavourzyme, and supernate is moved out; a 1 kDa ultrafiltration membrane is used for intercepting the mung bean peptides with the molecular cut off smaller than 1 kDa in the prepared supernate, desalination is conducted through the ultrafiltration membrane, and the immunological competence peptides of the mung beans can be obtained by conducting freezing drying on the processed filtrate. The mung bean peptides having different biological functions are prepared through one set of technical conditions, the process is simple and efficient, and the resource utilization rate is high.