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Low-temperature resistant lactobacillus with ACE (angiotensin converting enzyme) inhibitory activity

A technology of inhibitory activity and low temperature resistance, which is applied in the field of lactic acid bacteria, can solve the problems of not screening out Lactobacillus plantarum strains, and not measuring the ACE inhibitory activity of Lactobacillus plantarum, and achieves significant protease activity, obvious effect, and obvious antibacterial effect

Active Publication Date: 2013-03-27
HEILONGJIANG BAYI AGRICULTURAL UNIVERSITY
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Problems solved by technology

[0005] So far, there is no report on the assay of Lactobacillus plantarum ACE inhibitory activity, nor is it used as a relevant report on the development of meat starter strains, and there is no screening out the Report on Lactobacillus plantarum strains with ACE inhibitory activity and can be used as low-temperature fermented meat products

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  • Low-temperature resistant lactobacillus with ACE (angiotensin converting enzyme) inhibitory activity
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  • Low-temperature resistant lactobacillus with ACE (angiotensin converting enzyme) inhibitory activity

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specific Embodiment approach 1

[0021] The specific preservation address in this embodiment is No. 3, No. 1 Courtyard, Beichen West Road, Chaoyang District, Beijing, Institute of Microbiology, Chinese Academy of Sciences, postal code 100101.

[0022] In this embodiment, the low-temperature-resistant lactic acid bacteria with ACE inhibitory activity are isolated and purified from the kimchi purchased in Daqing Xinbaida Supermarket, and its samples are currently stored in the Microbiology Laboratory of the School of Food, Bayi Agricultural University, Heilongjiang; it is separated according to the following steps Purification culture: Kimchi was inoculated in MRS agar medium by dilution coating method, cultured at 37°C for 24 hours, and then the grown colony was further isolated and purified with MRS agar medium to obtain a pure strain, which was named pure strain I1 [namely Lactobacillus plantarum ( Lactobacillus plantarum )I1].

[0023] MRS agar medium (g / L): peptone 10g, beef powder 8g, yeast powder 4g, g...

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Abstract

The invention relates to a lactobacillus and in particular relates to a low-temperature resistant lactobacillus with ACE (angiotensin converting enzyme) inhibitory activity. The low-temperature resistant lactobacillus with the ACE inhibitory activity is lactobacillus plantarum I1 which is preserved in the China general microbiological culture collection center of which the preservation address is No.3, No1. yard, Beichen west road, Chaoyang District of Beijing, the preservation data is September 18th, 2012, and the preservation number is CGMCC No.6575; the lactobacillus plantarum I1 has the ACE inhibitory rate being 66%, can tolerate 6% of NaCl and 100mg / kg of nitrite, and can fast generate acid; the growing temperature of the lactobacillus plantarum I1 is 4-40 DEG C, and is suitable for low-temperature fermentation; and the low-temperature resistant lactobacillus has obvious protease activity and lipase activity, can not generate mucus, has an obvious antibacterial effect on staphylococcus aureus and colon bacillus, and can be used as a meat product leavening agent strain under a low-temperature condition.

Description

technical field [0001] The invention relates to a strain of lactic acid bacteria. Background technique [0002] Hypertension is a worldwide problem, affecting 15-20% of adults. Among the various factors that control blood pressure in the human body, renin-angiotensin system (Renia-angiotensin system, RAS) and kallikrein-kinin system (Kallikrein-kinin system, KKS) are two important adjustment system. Among them, angiotensin-converting enzyme (ACE) is an enzyme that plays a key role in the system regulation process and is the main factor that increases blood pressure. Inhibiting the activity of ACE can effectively prevent and treat the occurrence of hypertension. [0003] The research on screening microorganisms with ACE inhibitory activity and making them produce polypeptides with hypotensive function mainly focuses on the research on dairy products. However, the screening of strains with ACE inhibitory activity, low temperature resistance, salt resistance and nitrite res...

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Application Information

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Patent Type & Authority Applications(China)
IPC IPC(8): C12N1/20C12R1/25
Inventor 俞龙浩王欣
Owner HEILONGJIANG BAYI AGRICULTURAL UNIVERSITY
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