The invention discloses newly separated
pediococcus acidilactici HEW-AP27 collected in the CGMCC (China General
Microbiological Culture Collection Center) in the Institute of
microbiology of CAS (Chinese Academy of Sciences) in Beichen west road No.1
yard No.3 in Chaoyang District of Beijing with the
postal code of 100101 on March 7, 2018, with the collection number of CGMCC NO.15419. The invention also provides a preparation method of a viable
pediococcus acidilactici preparation. The method comprises the steps as follows: the
pediococcus acidilactici is used as a
fermentation strain, an optimized
fermentation process and a
fermentation medium are adopted, discharging is performed after first-stage, second-stage and third-stage culture, active bacterial
sludge is obtained through
centrifugation, a stabilization protective agent is added,
granulation is performed through a granulator, a
coating liquid is added to a
coating machine for
coating, and finally, the viable
pediococcus acidilactici preparation is obtained. The
pediococcus acidilactici HEW-AP27 has
acid resistance, cholate resistance and high-
temperature resistance, has high acid producing performance and can inhibit growth and
reproduction of
pathogenic bacteria.