146results about How to "Fast acid production" patented technology

The invention discloses a lactobacillus leavening agent, a preparation method thereof and a special bacterial strain. The preservation serial number of lactobacillus is CCTCC M208151. An active constituent of the lactobacillus leavening agent is the lactobacillus. The invention also discloses a method for preparing the lactobacillus leavening agent, which comprises the following steps: firstly, leavening and culturing lactobacillus plantarum SC79 CCTCC M208151 in SC79 optimal culture medium; and secondly, collecting thallus in the step 1 and then adding a protective agent to the thallus to obtain the leavening agent. The lactobacillus leavening agent has small dosage, quick acid production speed through mixing the lactobacillus leavening agent and a harvestless exopolysacchatide yoghourt bacterial strain to prepare yoghourt, better relative viscosity, elasticity, denseness and adhesiveness than that of the conventional yogurt and small syneresis sensibility of formed sticky yogurt colloids, larger water holding capacity than that the conventional yogurt and hard whey separation.

The invention discloses a greengage vinegar, preparation method thereof and application method thereof, belonging to the technical field of brewed food processing. The invention discloses a greengage vinegar, preparation method thereof and application method thereof, belonging to the technical field of brewed food processing. The greengage vinegar can also be used for preparing greengage vinegar drinks. The method adopts a new strain and a deep liquid fermenting process and has the advantages of short process, high labor productivity, and the like. The greengage vinegar and the vinegar drink which are prepared according to the method are clear and transparent, have strong and harmonious fruit and vinegar aroma, appropriate sour and soft taste and keeps the clear characteristics of greengage vinegars. {} The method can be popularized in brewing enterprises.

This invention relates to one method of improving fermentation efficiency of long chain diacid. In this method, normal alkane is the material, and animalcule fermentation method is adopted to produce correspondent diacid. Its major character is to add catastaltica, which includes halogeno-aliphatic acid, methyl fat acid and one or two kinds of its salt or ester, to reduce beta oxidation ability of bacterial strain. The concentration of the catastaltica is 0.01-5mmol/L. the added catastaltica can efficiently reduce beta oxidation ability of bacterial strain, lowers the alkane consumption, and improves greatly the fermentation efficiency, the acid-producing rapidity and is particularly suitable for long chain diacid.

The invention provides a method for producing L-ornithine by microbial fermentation, namely, using Corynebacterium glutamate to obtain the strains of CS-189(cit<(-)>+SG<r>) by chemical treatment, and obtain the L-ornithine hydrochloride by fermentation, culture solution micro-filtration by a ceramic membrane, ion exchange resin and extraction by a hydrothermal crystallization method with decompression concentration. The strains used in the method are auxotrophy resistant to sulfaguanidine, which significantly enhances acid yield by fermentation. Meanwhile, aiming at the problem that the solubility of the L-ornithine hydrochloride in water is extremely difficult, the hydrothermal crystallization method is adopted to obtain better extraction rate under the condition that flammable and explosive alcohol is not used. The method simplifies processes and reduces extraction cost.

A process for fermenting the fresh flower of marigold by bacterium lactis includes such steps as drying in the air, screening, loading it in fermenting pool, uniformly spraying composite bacterium lactis, compacting, sealing, and anaerobic fermenting at medium temp for 8-12 days. Its advantages are high acid generating speed and wall-breaking effect, and unique taste.

The invention relates to a fermentation medium for fermenting and producing glutamic acid from a thermo-sensitive type strain. The components and the final concentration of the fermentation medium in the formula are as follows: 165-205g/L corn starch amylolysis, 15-30g/L maize slurry, 10-18g/L molasses, 0.5-1.5g/L glycine betaine, 5-10g/L bean pulp hydrolysate, 3-8g/L H3PO4, 1.25-1.75g/L MgSO4.7H2O, 0.064-0.084g/L MnSO4.7H2O, 0.055-0.085g/L FeSO4.7H2O, 3-6g/L KCl, 0.08-0.15g/L threonine, 0.8-1.2g/L succinic acid, and 0.1-0.3g/L defoamer, and the solvent is water, the pH value of the fermentation medium is 6.8-7.0 when the phosphoric acid is neutralized by alkali. Due to the appropriate carbon source, the nitrogen source, the carbon nitrogen ratio and the phosphorus content in the medium, the acid production rate is increased, the acid production of the thallus is promoted, the final acid production level is obviously improved by about 5.5% and the glutamic acid yield and the glucose acid conversion ratio are increased.

The invention relates to a lactobacillus and in particular relates to a low-temperature resistant lactobacillus with ACE (angiotensin converting enzyme) inhibitory activity. The low-temperature resistant lactobacillus with the ACE inhibitory activity is lactobacillus plantarum I1 which is preserved in the China general microbiological culture collection center of which the preservation address is No.3, No1. yard, Beichen west road, Chaoyang District of Beijing, the preservation data is September 18th, 2012, and the preservation number is CGMCC No.6575; the lactobacillus plantarum I1 has the ACE inhibitory rate being 66%, can tolerate 6% of NaCl and 100mg/kg of nitrite, and can fast generate acid; the growing temperature of the lactobacillus plantarum I1 is 4-40 DEG C, and is suitable for low-temperature fermentation; and the low-temperature resistant lactobacillus has obvious protease activity and lipase activity, can not generate mucus, has an obvious antibacterial effect on staphylococcus aureus and colon bacillus, and can be used as a meat product leavening agent strain under a low-temperature condition.

The invention relates to the table vinegar production field, in particular to a method for composite acetic acid bacterium culture and solid-state acetic acid fermentation, which solves the problems that the acetic acid bacterium is simplex in strain, the acetic acid conversion rate is low, the traditional acetic acid bacterium culture method causes instability of table vinegar quality and the like in the prior art. The method comprises the following steps: utilizing Huniang 1.01, As1.41, bacterium gluconicum, acetobacter separated from a Shanxi mature vinegar grains and high-yield acetaldehyde dehydrogenase acetobacter to form the composite acetic acid bacteria, and then carrying out continuous culture in a composite culture medium comprising grape fruit juice, multi-strain nutrition-enhancing yeast, yeast extract, ethanol and distilled water to obtain a composite acetic acid bacteria culture solution; and finally carrying out solid-state acetic acid fermentation, wherein the inoculation process adopts operating techniques of mixing the grains at a pan bottom, drawing fire, burying in fire and rubbing the grains. The invention uses the advantages of multiple strains, raises the yield of gluconic acid in metabolite, produces a final product which is moderately sour and has unique flavor, improves the conversion rate of acetic acid, and shortens the fermentation period, thereby having profound significance in the progress of the table vinegar brewage technology.

The invention relates to a method for promoting the phosphorus release and the gas production of phosphate-precipitate-containing sludge under a room temperature condition. The method comprises: placing phosphate-precipitate-containing sludge in a reactor, carrying out static precipitation at a room temperature, and discharging the supernatant to obtain concentrated mixed sludge; adding trisodiumcitrate according to the total suspended solid content of the sludge, sealing the reactor, and carrying out anaerobic fermentation for more than 7 days under a room temperature condition, wherein thephosphorus release rate of the sludge can be increased, and the anaerobic fermentation of the sludge can be promoted so as to produce acid and methane. Compared to the method in the prior art, the method of the present invention has the following characteristics that the phosphorus release in the phosphate-precipitate-containing sludge can be promoted sp as to improve the recovery rate of the phosphorus resource at the late stage and promote the anaerobic fermentation of the sludge to produce acid and gas, and achieve the resource utilization of the residual sludge, and the operation is performed at the room temperature, such that the economic cost is low.

Microbe culture solution which contains a large number of viable bacteria is formed by fermenting microorganisms of which the main floras are acetic bacteria and yeasts; the microbe culture solution is mixed with a small amount of ethanol, acetic acid, sugar source, inorganic salt and the like; the mixture is added into a rubber latex; and latex particles are further solidified through the acetic acid and bacterial cellulose which are generated from nutrient substances in the rubber latex by the microorganisms, such as the acetic bacteria, yeasts and the like in the microbe culture solution. At the same time, the microorganisms decompose a large number of nutrient substances, such as a nitrogen source and the like, in the latex in a growing process, then the total solid content in solidified waste liquid is further reduced and pollution emission is reduced; and serum left in the solidified latex can serve as a culture medium of microorganism solidification liquid or a beneficial microbial ecological agent, and then the rubber latex is comprehensively used. The method has simple operation; by the method, the cost and the pollution are reduced and the rubber yield is improved; and the product produced by the method has better physical and chemical property than the product produced by acidic solidification.

The invention relates to a D-lactic acid produced strain and a method for producing D-lactic acid by using the same. When the D-lactic acid is produced by using the D-lactic acid produced strain Lp-DAprovided by the invention, the acid production speed and the optical purity of a product are obviously improved, namely, the D-lactic acid produced strain Lp-DA has obviously improved acid productionperformance for producing the D-lactic acid. Correspondingly, the production of the D-lactic acid can be improved greatly by using the D-lactic acid produced strain Lp-DA to produce the D-lactic acidthrough fermentation. Moreover, the D-lactic acid produced strain Lp-DA also has the advantages that the fermentation cost is low, the production cycle can be shortened, energy consumption required for temperature control in fermentation production is reduced, cooling water is saved, living contaminants are reduced and the method is environmentally-friendly.

The invention discloses a method for recycling sludge, comprising the following steps of: mixing the sludge with Ca(OH)2, placing the mixture into an fermentation reactor and stirring and performing anaerobic fermentation; discharging a certain volume of fermentation mixture from the reactor at regular time intervals, and adding fresh sludge in the same amount as the discharged mixture and a certain amount of Ca(OH)2; and adding a FeSO4 solution into the discharged mixture, stirred for a certain time, adding a polyacrylamide solution, stirring for a certain time, and then carrying out filter pressing for dewatering. The method remarkably improves the concentration and the fermentation liquor amount of short chain fatty acid generated by the sludge, decreases the volatile substances of the sludge, and meanwhile, can remove most phosphate in the fermentation liquor.

The invention relates to lactobacillus fermentum LG1 and an application thereof, wherein the collection number of the strain lactobacillus fermentum LG1 is CGMCC No. 7121; the registration number of the 16SrDNA gene sequence of the lactobacillus fermentum LG1 in a GenBank database is Accession No. KC348395; the lactobacillus fermentum LG1 CGMCC No. 7121 is applied to production for traditional millet fermented dough. A fermentation process for the millet dough can be rapidly finished via 6-8 hours of culture by taking the lactobacillus fermentum LG1 provided by the invention as a fermentation agent, and the quantity of the lactobacillus fermentum LG1 in the dough can be increased to 7.0-8.5 logcfu/g from 4.5-5.0 logcfu/g during inoculation; during production for traditional millet fermented dough products, a natural fermentation process for the millet dough generally needs 48-96 hours, therefore, by using the lactobacillus fermentum LG1, the fermentation period is greatly reduced.

The invention provides a method for producing organic acid at a high production rate through fermentation of intermittent backflow cells, which comprises the following steps of: performing filtration sterilization on a fresh culture medium, introducing into a fermentation tank, and inoculating high-density seed liquor into the fermentation tank; controlling organic acid fermentation production conditions of the used strain, when fermentation is performed until a small amount of sugar residue is in fermentation liquor, discharging partial or all fermentation liquor to a filtering device for processing, simultaneously supplementing a fresh germ-free culture medium into the fermentation tank, returning intercepted strain cell concentrated solution to the fermentation tank to serve as a production strain for later use, wherein the filtered liquor is used for extracting organic acid; and only when the production rate of the organic acid is obviously reduced, not returning the strain cell concentrated solution intercepted by the filtering device is not to the fermentation tank any more, and supplementing new high-density seed liquor and a fresh germ-free fermentation culture medium into the fermentation tank so as to perform a new round of production of organic acid at the high production rate through fermentation of the intermittent backflow cells. Through the method, high biomass and high production rate are always kept in the organic acid fermentation production process, and the method has high industrial application value.

The invention discloses a Lactobacillus paracasei 761, application of the Lactobacillus paracasei 761, a silage additive and silage. The Lactobacillus paracasei 761 is deposited at China General Microbiological Culture Collection Center, and the deposit number is CGMCCNo.18234; the Lactobacillus paracasei 761 can improve the quality of the silage in high-temperature and high-humidity areas, and the silage treated by the Lactobacillus paracasei 761 has better aerobic stability, can reduce pH, aflatoxin B1 and dry matter loss and has good high-temperature resistance and acid resistance and goodadaptability to the environment, under culturing in high-temperature conditions, the growth and acid production of the Lactobacillus paracasei 761 has no significant lag phase, and can achieve rapid acid production to achieve the effect of lower pH .

The invention discloses a candida tropicalis strain CAT H1614, a microbial agent comprising the same and an application thereof, and a method for producing long chain diprotic acid by fermentation anda preparation method of the long chain diprotic acid. The candida tropicalis strain CAT H1614 of the invention can maintain high vitality for a long time in an environment with a pH lower than 7.0, thereby being particularly suitable for production of the long chain diprotic acid by a biological method, acid production is rapid during fermentation, the yield is high, and a fermentation cycle canalso be reduced. The fermentation method and the preparation method of the long chain diprotic acid provided by the invention have significant cost advantages, can effectively alleviate pressure on resources and environment, and thus have very obvious industrialization value advantages.

The invention relates to a lactic acid producing strain and a method for producing lactic acid through the lactic acid producing strain. When the lactic acid is produced through the lactic acid producing strain namely pediococcus acidilactici Pa-COT, the lactic acid production speed is significantly increased, namely that the lactic acid producing strain Pa-COT can significantly increase the lactic acid production property during lactic acid production. Accordingly, when the lactic acid is produced through fermentation of the lactic acid producing strain Pa-COT, the production of the lactic acid can be greatly improved. Besides, the lactic acid producing strain Pa-COT further has the advantages of being low in fermentation cost and environmental-friendly. In addition, the lactic acid producing strain Pa-COT disclosed by the invention further has the following advantages that the lactic acid can be produced through normal fermentation at the fermentation temperature of 45-50 DEG C, thelactic acid producing strain has very good adaptability to a lignocellulose system and can bear stayers generated in the high-concentration preprocessing process, and lactic acid fermentation can be performed by using lignocellulose as a raw material.

The invention relates to pure natural lactobacillus millet enzyme. The pure natural lactobacillus millet enzyme is prepared from the following steps: preparing powder: smashing millet, and screening by a 100-mesh sieve; carrying out saccharification liquid preparation: weighing 200g of obtained millet powder, adding 1000mL of water, heating to 50 DEG C, adding glucoamylase for saccharification, heating to 85DEG C after saccharification is carried out, and maintaining for 10 minutes; blending and inoculating: weighing 1000g of obtained millet powder, adding 4000mL of water, adding saccharification liquid of which the water volume is 10-20%, and inoculating lactobacillus fermentium, lactobacillus reuteri, lactobacillus plantarum and bifidobacterium adolescentis at the ratio of 3:1:2:1; fermentation: sealing a fermentation container, carrying out fermentation for 90 days under the condition of 28-35 DEG C, and carrying out after-ripening fermentation for 30 days under the condition of 4-10 DEG C, thus obtaining the pure natural lactobacillus millet enzyme after fermentation ends. No food additive is added into the pure natural lactobacillus millet enzyme, and the pure nature and the safety of food can be guaranteed furthest.

The invention discloses an inulin low-fat yogurt pudding and a preparation method thereof, and belongs to the technical field of food processing. The inulin low-fat yogurt pudding is prepared from the following components by weight percent: 30-40% of yogurt base, 2-10% of inulin, 0.05-0.5% of a thickener, 0.001-5% of a sweetening agent and the balance of water, wherein the inulin is a soluble dietary fiber, can selectively promote growth of colon probiotics, and has the functions of reducing blood sugar concentration, maintaining lipid metabolism balance, improving the bioavailability of mineral elements, enhancing the immunity of the organism, and the like. The inulin low-fat yogurt pudding disclosed by the invention contains the inulin dietary fiber, is low in fat content, and smooth in mouthfeel, has the functions of improving the intestinal microenvironment, controlling blood fat and blood sugar levels, reducing the energy, promoting absorption of a mineral substance and synthesis of vitamins, and is especially suitable for middle aged and elderly people with diseases such as constipation, obesity, hypertension, hyperlipidemia and diabetes mellitus.

Potato yoghourt and a making method thereof are disclosed. 40-60% of potato juice is creatively added into yoghourt, and lactobacillus bulgaricus and streptococcus thermophilus are used in cooperation for fermentation. On the basis of controlling the ratio of the materials and the making method, the potato yoghourt with rich nutrients, moderate sweetness and palatable sour taste is prepared.