Pediococcus acidilactici strain high in stress resistance and capable of utilizing various carbon sources, and method for producing lactic acid through strain

A technology of Pediococcus lactis and lactic acid, applied in the field of microbial fermentation, to achieve the effects of increased acid production speed, good adaptability, and low fermentation cost

Active Publication Date: 2019-03-29
JILIN COFCO BIOCHEM +2
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

[0006] However, there is currently considerable room for improvement in thermotolerant lactic acid producing strains capable of producing lactic acid from multiple carbon sources at high yields

Method used

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  • Pediococcus acidilactici strain high in stress resistance and capable of utilizing various carbon sources, and method for producing lactic acid through strain
  • Pediococcus acidilactici strain high in stress resistance and capable of utilizing various carbon sources, and method for producing lactic acid through strain
  • Pediococcus acidilactici strain high in stress resistance and capable of utilizing various carbon sources, and method for producing lactic acid through strain

Examples

Experimental program
Comparison scheme
Effect test

Embodiment 1

[0045] Example 1: Strain isolation, high temperature resistant strain screening and strain identification

[0046] Strain isolation: mash 1g Daqu sample and dissolve it with 10mL sterile saline, mix well for 30min; take the above dilution 10-fold and apply it to 1% (w / v) CaCO 3 (Neutralizer) MRS solid medium, culture at 37°C for 24 hours, pick a single colony with a larger transparent circle to inoculate the screening medium, incubate at 37°C for 24 hours, measure the total lactic acid content with high performance liquid chromatography, select lactic acid High-yield strains are lactic acid bacteria with high lactic acid content in the fermentation product.

[0047] Screening of high-temperature resistant strains: Select a batch of lactic acid-producing strains from the above-isolated lactic acid bacteria, inoculate MRS medium, culture at 37°C at 150 rpm for 1 hour, take 5μl of culture medium and spot it on MRS+CaCO 3 On a solid plate, place the plate in an incubator at 45°C for 40 ...

Embodiment 2

[0052] Example 2: Strain fermentation to produce lactic acid

[0053] The lactic acid producing strain Pa-COT obtained in Example 1 was inoculated with MRS medium and cultured overnight at 37°C at 150 rpm to obtain seed liquid. Then, inoculate the above-mentioned seed liquid at a ratio of 10% (v / v) in 200 mL of acid-producing fermentation medium containing different carbon sources (100 g / L glucose, 100 g / L xylose or 40 g / L arabinose, respectively), Cultivate the strain on a shaker at 37°C and 150rpm for 3-5 hours to allow the strain to grow, and then increase the temperature to 45°C or 48°C, and continue to cultivate on a shaker at 150rpm for 72 hours to obtain a fermentation broth. After the fermentation, the lactic acid production was measured by high performance liquid chromatography.

[0054] As a result, when different monosaccharides are used as carbon sources, the Pediococcus lactis strain Pa-COT can ferment to produce 100.9g / L of lactic acid in 72 hours when using glucose ...

Embodiment 3

[0055] Example 3: Strains use cellulose hydrolysate to produce lactic acid

[0056] In this example 3, a straw enzymatic hydrolysate medium was used as an acid-producing fermentation medium, which contained the following components: straw enzymatic hydrolysate (the main components are glucose, xylose and arabinose, used as a carbon source), 5% (v / v) Concentrated corn steep liquor (about 60% water content), KH 2 PO 4 1g / L, MgSO 4 ·7H 2 O 0.5g / L, MnSO 4 ·H 2 O 0.25g / L, CaCO 3 50g / L, pH is about 6.

[0057] The lactic acid producing strain Pa-COT was inoculated with MRS medium and cultured overnight at 37°C at 150 rpm to obtain seed liquid. Take 20ml of the seed solution and centrifuge for 5min at 6000rpm, discard the supernatant, and inoculate 200ml of straw enzymatic hydrolysis medium with 1ml of saline suspension. Cultivate for 4 hours on a shaker at 37°C and 180rpm to allow the strain to grow rapidly, and then warm to 45°C, continue Incubate for 92 hours on a shaker at 180 rpm...

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Abstract

The invention relates to a lactic acid producing strain and a method for producing lactic acid through the lactic acid producing strain. When the lactic acid is produced through the lactic acid producing strain namely pediococcus acidilactici Pa-COT, the lactic acid production speed is significantly increased, namely that the lactic acid producing strain Pa-COT can significantly increase the lactic acid production property during lactic acid production. Accordingly, when the lactic acid is produced through fermentation of the lactic acid producing strain Pa-COT, the production of the lactic acid can be greatly improved. Besides, the lactic acid producing strain Pa-COT further has the advantages of being low in fermentation cost and environmental-friendly. In addition, the lactic acid producing strain Pa-COT disclosed by the invention further has the following advantages that the lactic acid can be produced through normal fermentation at the fermentation temperature of 45-50 DEG C, thelactic acid producing strain has very good adaptability to a lignocellulose system and can bear stayers generated in the high-concentration preprocessing process, and lactic acid fermentation can be performed by using lignocellulose as a raw material.

Description

Technical field [0001] The invention relates to the field of microbial fermentation. Specifically, the present invention relates to a strain of Pediococcus lactis that is highly resistant and can use various carbon sources to ferment to produce lactic acid, and a method for producing lactic acid using the strain of Pediococcus lactis. Background technique [0002] Polylactic acid (PLA) is a biodegradable polymer formed by condensation polymerization of lactic acid monomers. Because its raw materials are renewable biological resources, PLA has been unanimously recognized by the industry as the most promising new "bio-based material" in the new century. In addition, the products made of PLA have good gloss, transparency, hand feeling and heat resistance, and also have certain bacteria resistance, flame retardancy and UV resistance, and have high gloss and processing performance; PLA also It has the characteristics of non-toxic, non-irritating and good biocompatibility. Therefore...

Claims

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Application Information

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Patent Type & Authority Applications(China)
IPC IPC(8): C12N1/20C12P7/56C12R1/01
CPCC12N1/20C12P7/56C12R2001/01C12N1/205
Inventor 佟毅张媛焦琳王小艳郑晓卫靳喜庆李义陈博丁子元王燕刘志刚
Owner JILIN COFCO BIOCHEM
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