27results about How to "Reduce fermentation costs" patented technology

The invention belongs to the technical field of microbial fermentation, and particularly relates to a fermentation substrate for bacillus, a bacillus liquid fermentation inoculant and application. The fermentation substrate is prepared by adding traditional Chinese medicine residues into a yeast culture medium so as to improve the concentration of bacillus after fermentation; the traditional Chinese medicine residues are prepared by compounding motherwort herb residues, astragalus membranaceus herb residues and ginseng herb residues according to the mass ratio of 1: (1-2): (1-1.5); the mass ratio of the yeast culture medium to the traditional Chinese medicine residues is (20-26): (6-8). The concentration of the bacillus can reach 3.3 * 10 < 9 > CFU / mL in a bacillus liquid fermentation inoculant obtained by adopting the fermentation substrate disclosed by the invention.

The invention discloses a recombinant IV-type collagen with anti-inflammatory activity and a preparation method and application thereof, the recombinant IV-type collagen is a truncated protein of natural human IV-type collagen, and the amino acid sequence of the recombinant IV-type collagen is shown as SEQ ID NO: 1. The recombinant IV-type collagen has a remarkable anti-inflammatory effect, can regulate inflammatory factors, relieve and improve inflammatory symptoms, has anti-inflammatory and soothing effects, and can be used as a biomedical material to be applied to preparation of skin care products, medicines or medical instruments with anti-inflammatory activity.

The invention discloses a strain of Lactobacillus brevis as well as a high-density culture method and application thereof, the strain is Lactobacillus brevis X66, is obtained by separating and screening from a traditional dairy product dried milk cake in Inner Mongolia, is preserved in Guangdong Microbial Culture Collection Center on August 4, 2022, and has a preservation number of GDMCC No: 62641. The strain provided by the invention is safe, can tolerate gastrointestinal tract environment, has excellent self-coacervation capability and oxidation resistance, and can inhibit escherichia coli, staphylococcus aureus and pseudomonas aeruginosa. The invention provides a high-density culture method of the X66 strain, the viable count of X66 in optimized fermentation liquor exceeds 2 billion CFU / mL and is 2-3 times that before optimization, the viable count of freeze-dried powder prepared from the X66 strain reaches up to 10 billion CFU / g, and preconditions are provided for application of the strain in the fields of fermented dairy products, functional foods, nutritional dietary supplements and the like.

The invention discloses a fresh cut flower preservative and a preparation method and application thereof.Auricularia auricula polysaccharide and 8-hydroxyquinoline are adopted as main components for preservation, the balance is water to prepare a mixed aqueous solution, and bacterial nanocellulose (BNC) produced by protein waste liquid is adopted as a medium of the aqueous solution; and after water absorption saturation, obtaining the functional fresh-keeping material for the fresh cut flowers. The bacterial nano cellulose is biodegradable and non-toxic, and the fresh cut flower preservation gel prepared by the method is uniform and stable, is a semi-solid, is not easy to flow, and is easy to subpackage, transport and use. Compared with water-retaining cotton and flower mud, the water-retaining agent is longer in water-retaining time and easier to decompose, and the loss of an aqueous solution is reduced. The fresh-keeping period of the fresh cut flowers can be prolonged after the roots of the fresh cut flowers are placed in the packaging bag for fresh-keeping and sealing, and the packaging bag can be used for long-distance transportation of the fresh cut flowers and water retention of bouquets in flower stores.

The present invention provides a sludge hydrolysis and acidification carbon source supplementation device, comprising a fermentation tank, a feeding channel and a stirring tank; the upper part of the feeding channel is provided with a sludge input port and a mixed liquid input port; the lower part of the feeding channel is provided with a dispersion channel, a dispersion plate is provided between the dispersion channel and the stirring tank, one or more first augers are provided in the dispersion channel, one or more stirring mechanisms are provided in the stirring tank, a stirring paddle and a second augers are provided on the stirring mechanism, a pH detection mechanism and an oxidation-reduction potential detection mechanism are provided on the upper part of the dispersion plate, and a liquid addition mechanism is provided in the stirring tank. The present invention increases the activity of acid-producing bacteria in sludge, inhibits the activity of methanogens, increases the amount of carbon source produced by sludge fermentation, and recycles acid and carbon tetrachloride by controlling the pH value and the oxidation-reduction potential, and each space of the present invention can be manually entered for cleaning, which facilitates the subsequent maintenance of the entire device.

The invention relates to fermented soybean meal as well as a preparation method and application thereof, and the preparation method comprises the following steps: mixing all fermentation raw materials required by fermentation of the current batch to prepare a fermentation material; and fermenting the fermentation material until the content of a target component in an obtained fermentation system reaches a preset level, and preparing fermented soybean meal, wherein the fermentation raw materials comprise fermentation water, crushed soybean meal, zymophyte and enzyme; the fermentation water contains condensate water obtained by condensing dry tail gas generated by drying other batches of fermented soybean meal. According to the application, the fermented soybean meal is prepared in a biological fermentation and enzymolysis combined manner, and condensate water obtained by condensing dry tail gas generated by drying other batches of fermented soybean meal is added into the fermentation water, so that the recycling of the condensate water is realized; the time consumed from fermentation to the time when the content of the target component in the obtained fermentation system reaches the preset level is not obviously prolonged, the utilization rate of fermentation equipment is increased, and the fermentation cost is reduced.

A filtering device for a reduced indigo solution comprises a tank body and a filtering assembly, the tank body comprises an upper-section tank body and a lower-section tank body, a reduced indigo solution inlet, a protective gas source inlet and a protective gas source outlet are formed in the top of the upper-section tank body, a discharging opening is formed in the bottom of the lower-section tank body, and a first flange plate is arranged at an opening in the lower end of the upper-section tank body; the upper end face of the upper-section tank body is provided with a first flange plate, the lower end face of the first flange plate is provided with an annular opening pressing groove, the upper end opening of the lower-section tank body is provided with a second flange plate, and the upper end face of the second flange plate is provided with an annular opening supporting groove; the filter partition plate is supported in the annular opening supporting groove, the pressing ring is limited by the annular opening pressing groove, and the filter cloth is supported on the filter partition plate and positioned by the pressing ring. The reduction indigo solution filtering device is simple in structure and convenient to maintain, oxidation can be effectively avoided on the basis of filtering a reduction indigo solution, and the quality of the reduction indigo solution is guaranteed.

The invention relates to the field of genetic engineering and fermentation engineering, and particularly discloses a method for exogenous expression of COQ3 by recombinant pichia pastoris engineering bacteria. According to the invention, a target gene COQ2 is inserted into a pichia pastoris secretory expression vector ppicZA to construct a recombinant expression plasmid. The plasmid is transformed into a pichia pastoris GS115 host bacterium to obtain a recombinant pichia pastoris engineering bacterium capable of expressing COQ3. Through methanol-induced fermentation, the engineering bacterium can accumulate COQ3 in cells. The method provided by the invention has the advantages of low cost, easiness in large-scale production and the like, and provides a new way for realizing industrial production of COQ3 and other ubiquinone substances.

The invention relates to the technical field of microorganisms, in particular to a fish streptococcicosis-resistant feed additive and application thereof.The strain D7-HSJ9 applied to the additive is obtained by screening of an applicant, it is detected that the strain has good salt resistance, alkali resistance and antibacterial characteristics, especially has a good antibacterial effect on streptococcus, and after processing, the strain can be used for preparing a feed additive for fish streptococcicosis. The feed additive prepared by compounding the strain and traditional Chinese medicines can well inhibit the growth of streptococcus iniae and streptococcus agalactiae, the additive is used for preparing disease-resistant feed, and the additive has the remarkable advantages of being scientific in component compatibility, obvious in synergistic effect, capable of meeting the growth requirement of fish, capable of remarkably promoting the growth of the fish, capable of improving the feed utilization rate and capable of reducing the feed conversion ratio. In addition, the disease-resistant feed also has the effects of effectively improving the immunity of the fish and reducing the morbidity of the fish, and is a biological feed additive and a biological feed which are low in cost and safe in color.

The utility model discloses a special fermentation device for lactic acid bacteria. The device is mainly divided into three parts, namely a fermentation tank body, an ozone sterilization device and an external circulation ultraviolet sterilization device, a tank cover is mounted at a top opening of the fermentation tank body, tank legs are arranged on the periphery of the bottom of the fermentation tank, a discharge port communicated with the inside and the outside of the fermentation tank is formed in the center of the bottom of the fermentation tank, and the fermentation tank is provided with a pH detection device and a temperature control device. According to the metabolic characteristics of lactic acid bacteria, equipment of the fermentation tank is optimized, a stirring device of the fermentation tank is reduced, the mass transfer effect is achieved through the external circulating pump, and high-temperature and high-pressure steam sterilization is replaced by a mode of combining ozone sterilization and ultraviolet sterilization; therefore, the equipment is further utilized to achieve the effect of uniform fermentation liquor while sterilization is achieved, the fermentation cost is reduced, the safety of the fermentation process is improved, and the method is generally suitable for lactobacillus fermentation.

The invention relates to the field of microbial fermentation, in particular to a fermentation medium for improving the yield of astaxanthin produced by yarrowia lipolytica and a method for producing astaxanthin at high yield by using the fermentation medium. The invention provides a fermentation culture medium for improving the yield of astaxanthin produced by yarrowia lipolytica, and when the fermentation culture medium provided by the invention is used for producing astaxanthin, a large amount of high-activity astaxanthin can be obtained. The invention also provides a method for increasing the yield of astaxanthin produced by yarrowia lipolytica based on the fermentation medium provided by the invention, in the method, the astaxanthin is produced by fermenting the yarrowia lipolytica, citric acid is added in different stages of thallus growth, so that more precursor acetyl coenzyme A flows to an astaxanthin synthesis route, and the yield of the astaxanthin is increased. By adopting the method, the yield of the astaxanthin is greatly improved, the fermentation cost is reduced, the fermentation period is shortened, the generated astaxanthin has a 100% levorotatory configuration, the biological activity is high, and the method has a wide application prospect in the field of feeds.

The invention discloses an underwater image depth-of-field prediction method and system based on the Lambert-Beer law, and the method comprises the steps: carrying out the preprocessing of a to-be-predicted underwater image, and obtaining a noise reduction image; according to the Lambert-Beer law, calculating a first prediction distance and a second prediction distance corresponding to each pixel point in the noise reduction image; wherein the first prediction distance and the second prediction distance are dual-channel light propagation distances corresponding to pixel points; and according to the first prediction distance and the second prediction distance corresponding to each pixel point, respectively calculating to obtain a depth value of each pixel point. According to the invention, the efficiency of underwater image depth information prediction can be improved.

The present invention belongs to the technical field of microbial fermentation in the light industry, and discloses a method for producing L-theanine by fermentation, which mainly includes: accessing a microbial strain to a seed culture medium for cultivation, and obtaining a seed solution by controlling the culture conditions; inoculating the seed solution into a fermentation tank by a shake flask inoculation method for fermentation, and adding ethylamine in batches and adding sugar during the fermentation process to obtain a high-acid fermentation liquid. The present invention has high fermentation efficiency, low investment cost, and accurately controls the fermentation process to avoid fermentation out of control and cost increase, while reducing labor intensity, and the entire production process is simple and easy to operate.

The utility model relates to vibrating membrane type cosmetic raw material grading filtering equipment which comprises a bottom plate, a mounting plate, a filtering part and a power part, the mounting plate is movably connected with the bottom plate through a guide shaft and a push rod of a push piece, the filtering part and the power part are both located on the mounting plate, the middle of the filtering part is in contact with the mounting plate, and the power part is located on the mounting plate. The end part of the filtering part is movably connected with the mounting plate through a power part; a limiting part is arranged on the mounting plate, the middle part of the filtering part is movably connected with the mounting plate through the limiting part, and the filtering part is matched with the limiting part in size; the filtering part comprises a plurality of filtering membranes, and a water outlet pipe and a water inlet pipe are arranged on the filtering part; the power component comprises a positioning plate, a first reset piece, a second reset piece and a power piece. The utility model provides vibrating membrane type cosmetic raw material grading filtering equipment, which reduces the cleaning times of a filtering membrane and improves the filtering efficiency.

This invention discloses a thermophilic recombinant *Yersinia lipolytica* strain producing carotenoids, its construction method, and its applications. The recombinant strain exogenously expresses thermophilic precursor fatty acid desaturase (FAD3) and isocitrate dehydrogenase (IDH2) mutants from *Rhodotorula rubrum*, achieving efficient synthesis at 30°C. Secondly, the metabolic pathway of the recombinant strain is optimized based on a metabolic flux push-pull strategy, overexpressing isopentenyl pyrophosphate isomerase (IDI), farnesyl pyrophosphate synthase (ERG20), phytene dehydrogenase (CarB), and bifunctional phytene synthase (CarRP) to increase precursor throughput. Finally, multiple copies of the genes for astaxanthin synthase (crtS) and cytochrome P450 reductase (crtR) from *Rhodotorula rubrum* are performed to further enhance the carotenoid production capacity of *Yersinia lipolytica*. The thermophilic *Yersinia lipolytica* construction method of this invention is simple, can achieve more efficient carotenoid synthesis at higher temperatures, significantly reduces fermentation costs, and has potential for industrial production.

The invention relates to the field of microbial fermentation, in particular to a saccharification and liquefaction bifunctional recombinant yeast strain as well as a construction method and application thereof. The invention provides a recombinant yeast strain with saccharification and liquefaction dual functions, and the recombinant yeast strain has better clinker fermentation and raw material fermentation capabilities through co-expression of high-enzyme-activity alpha-amylase and glucoamylase genes. Under the conditions of clinker fermentation and raw material fermentation, the fermentation alcohol content of existing strains can be improved, addition of exogenous enzymes is reduced, cost reduction and yield increase of biological raw material ethanol can be achieved, and the large-scale industrial production requirement is met.

The invention discloses a biosynthesis method of alpha-lipoic acid, an engineering strain and a preparation method of the engineering strain. The construction of the engineering strain comprises the following steps: transforming lipocaprylamide enzyme Lpa of enterococcus faecalis, constructing the lipocaprylamide enzyme Lpa to an arabinose promoter or a Cold temperature-sensitive promoter, and then replacing the lipocaprylamide enzyme Lpa to a LacZ gene and an lpxM gene of escherichia coli through RED recombination; or the genes of LipD, lplA, lipA and lpA which are subjected to gene code modification are expressed in the saccharomyces cerevisiae through a pESC vector. The engineering strain is cultured to biologically synthesize alpha-lipoic acid, so that the fermentation cost is further reduced, cyclic synthesis is realized, and the yield is greatly increased.

The utility model provides an active ink negative pressure filtering system which comprises a filtering device and a liquid storage tank, the filtering device is communicated with the liquid storage tank, a negative pressure pipe is arranged on the liquid storage tank, the filtering device comprises a funnel, a first supporting screen plate is arranged on the funnel, the first supporting screen plate is of a net structure arranged in a wire coiling mode, and a filtering membrane can be placed on the first supporting screen plate. An electric reciprocating track is arranged on one side of the funnel and extends in the length direction of the first supporting screen plate, an ink spraying pipe is arranged on the electric reciprocating track, a spraying head is arranged at one end of the ink spraying pipe and located over the first supporting screen plate, and the ink outlet end face of the spraying head faces the first supporting screen plate. The other end of the ink spraying pipe is communicated with ink to be filtered, and the ink spraying pipe can move along the electric reciprocating track. The active ink negative pressure filtering system is high in filtering efficiency, easy to operate and high in automation degree.

This invention discloses an engineered Bacillus subtilis strain producing tagatose, its construction method, and its applications, belonging to the field of bioengineering technology. This engineered strain uses Bacillus subtilis as a host and co-expresses an L-arabinose isomerase from *Erysipelothrix larvae* and a β-galactosidase from *Escherichia coli*. The two genes are tandemly linked via ribosome binding sites or promoters to construct a Bacillus subtilis strain with dual-enzyme co-expression. This dual-enzyme Bacillus subtilis strain catalyzes the production of D-tagatose from lactose throughout the entire cell. After optimization of the enzymatic reaction, the yield and conversion efficiency of D-tagatose are significantly improved. This invention employs single-strain dual-enzyme co-expression and one-step whole-cell catalysis, using low-cost lactose as a substrate. The reaction is mild, with few byproducts, simplified process, and no endotoxin safety hazards. It solves the problems of low conversion rate, high substrate cost, and cumbersome strain formulation in existing technologies, making it suitable for the industrial production of food-grade D-tagatose.

The utility model discloses a kind of electronic grade hydrogen fluoride acid impurity removal pipe fittings.The utility model, including first pipe body: the middle position in the inside of first pipe body is provided with first connecting piece, the top of first connecting piece is rotatably provided with first flow guide fan blade, the bottom end in the inside of first pipe body is provided with first filter screen, the bottom of first pipe body is provided with second pipe body, the bottom end in the inside of second pipe body is provided with second filter screen.The utility model, by the splicing between first pipe body, second pipe body, third pipe body and fourth pipe body constitutes impurity removal pipe fitting, and multiple filter screens are provided in pipe fitting, improve the quality and quality of electronic grade hydrogen fluoride acid, since first pipe body, second pipe body, third pipe body and fourth pipe body can be split, filter screen can be disassembled and cleaned, solve the problem that filter screen is blocked when cleaning, meanwhile, the impurity removal pipe fitting is relatively small in size, reduce the filtering cost.

The utility model discloses a modularized composite chemical filter, which is characterized in that a shell is provided with a through hole pair, a V-shaped frame and a buckling seat, the V-shaped frame comprises two positioning boxes which are connected to form a V shape, each positioning box is provided with a mounting port and a ventilation window, the mounting ports of the two positioning boxes in the V-shaped frame are adjacent, and the buckling seat is arranged on the V-shaped frame. Each through hole pair is located at the corresponding two adjacent mounting ports; the chemical filtering modules are arranged in the corresponding positioning boxes; the filter increasing assembly comprises a filter screen and two buckling pieces, the filter screen is located on the side, close to the through hole pair, of the V-shaped frame, and the two opposite ends of the filter screen are clamped to the two buckling structures correspondingly; the baffles penetrate through the corresponding through hole pairs and are located on the sides, close to the mounting openings, of the corresponding chemical filtering modules, and the filter screens are further clamped between the baffles and the V-shaped frame. According to the utility model, multi-stage filtration can be realized, the air filtration and purification effect is good, the disassembly, assembly, maintenance and operation are convenient, the chemical filtration module can be independently replaced, and the use cost is low.

The present invention belongs to the technical field of fermentation engineering, and discloses a mulberry endophyte producing 1-deoxynojirimycin, a separation and purification method and application thereof. The strain is Methylobacterium zasneri, which was deposited in the General Microbiological Center of the China Microbiological Culture Collection Administration Committee on August 29, 2022. The deposit address is No. 3, Yard No. 1, Beichen West Road, Chaoyang District, Beijing, and the deposit number is CGMCC No. 25606. The strain of the present invention is separated from the mulberry endophyte by a simple separation and purification method, and is applied to fermentation engineering. By optimizing the fermentation conditions, DNJ can be quickly and highly produced, with a yield of up to 51.6 μg / mL. DNJ is used in the field of industrial production of products with hypoglycemic function after extraction and purification.

The application discloses a genetically engineered bacterium and a preparation method and application thereof. The genetically engineered bacterium expresses a cystathionine gamma-lyase gene, wherein the cystathionine gamma-lyase is NCBI accession number XP_011387340.1, WP_015753539.1 and / or NP_009390.1. The genetically engineered bacterium provided by the application forms a new cysteine supply path, solves the problem of sulfur source supply in the production of ergothioneine by fermentation, and simultaneously saves the fermentation cost without additional addition of cysteine.

This invention relates to the field of microbial fermentation, and particularly to a recombinant yeast strain with dual functions of saccharification and liquefaction, its construction method, and its applications. This invention provides a recombinant yeast strain with dual functions of saccharification and liquefaction. Through the co-expression of high-activity α-amylase and saccharifying enzyme genes, it exhibits superior fermentation capabilities for both cooked and raw materials. Under both cooked and raw material fermentation conditions, it can increase the fermentation alcohol content of existing strains and reduce the addition of exogenous enzymes, enabling cost reduction and increased production of ethanol from bio-based raw materials, meeting the needs of large-scale industrial production.

The invention belongs to the technical field of fermentation engineering, and relates to a method for improving the fermentation yield and conversion rate of L-proline. According to the method, the control process of controlling the proper residual sugar concentration in the fermentation process and combining heating fermentation and organic nitrogen source feeding is adopted, the yield of L-proline can be 150 g / L or above, the yield is 4 g / L / h or above, the conversion rate is 43% or above, the content of heteroacid glutamic acid is low and reaches the best level reported in China at present, the fermentation cost of the process is obviously reduced, and the method is suitable for industrial production.

The present invention provides a fermentation method of yeast for Streptomyces aureus fermentation, a fermentation product and an application thereof. The present invention provides a fermentation method of yeast for Streptomyces aureus fermentation, which helps to increase yeast yield and reduce the fermentation cost of Streptomyces aureus. At the same time, the present invention also provides a method for processing yeast fermentation products, by centrifugally concentrating the yeast fermentation products, directly using the yeast milk obtained by centrifugation concentration as a nitrogen source for the fermentation of Streptomyces aureus, and using the supernatant produced by centrifugation concentration as a nitrogen source or a feed culture medium solvent, thereby solving the problems of complicated post-processing steps, high energy consumption and wastewater treatment of yeast fermentation products, reducing the fermentation cost of Streptomyces aureus and alleviating environmental pressure.

The invention provides application of tributyrin as an additive in improving the fermentation yield of vanillin synthesized by a microbiological method and an additive for improving the fermentation yield of vanillin synthesized by the microbiological method, and belongs to the technical field of additives. According to the invention, tributyrin is used as an additive for improving the fermentation yield of the vanillin synthesized by the microbiological method, and the toxicity of the vanillin to thalli can be reduced, so that the fermentation yield of the vanillin is effectively improved. The method is suitable for all microorganisms, and the adding volume proportion and the adding time after fermentation are not limited.