8results about How to "Reduce fermentation costs" patented technology

The invention relates to the field of genetic engineering and fermentation engineering, and particularly discloses a method for exogenous expression of COQ3 by recombinant pichia pastoris engineering bacteria. According to the invention, a target gene COQ2 is inserted into a pichia pastoris secretory expression vector ppicZA to construct a recombinant expression plasmid. The plasmid is transformed into a pichia pastoris GS115 host bacterium to obtain a recombinant pichia pastoris engineering bacterium capable of expressing COQ3. Through methanol-induced fermentation, the engineering bacterium can accumulate COQ3 in cells. The method provided by the invention has the advantages of low cost, easiness in large-scale production and the like, and provides a new way for realizing industrial production of COQ3 and other ubiquinone substances.

This invention discloses a thermophilic recombinant *Yersinia lipolytica* strain producing carotenoids, its construction method, and its applications. The recombinant strain exogenously expresses thermophilic precursor fatty acid desaturase (FAD3) and isocitrate dehydrogenase (IDH2) mutants from *Rhodotorula rubrum*, achieving efficient synthesis at 30°C. Secondly, the metabolic pathway of the recombinant strain is optimized based on a metabolic flux push-pull strategy, overexpressing isopentenyl pyrophosphate isomerase (IDI), farnesyl pyrophosphate synthase (ERG20), phytene dehydrogenase (CarB), and bifunctional phytene synthase (CarRP) to increase precursor throughput. Finally, multiple copies of the genes for astaxanthin synthase (crtS) and cytochrome P450 reductase (crtR) from *Rhodotorula rubrum* are performed to further enhance the carotenoid production capacity of *Yersinia lipolytica*. The thermophilic *Yersinia lipolytica* construction method of this invention is simple, can achieve more efficient carotenoid synthesis at higher temperatures, significantly reduces fermentation costs, and has potential for industrial production.

The invention relates to the field of microbial fermentation, in particular to a saccharification and liquefaction bifunctional recombinant yeast strain as well as a construction method and application thereof. The invention provides a recombinant yeast strain with saccharification and liquefaction dual functions, and the recombinant yeast strain has better clinker fermentation and raw material fermentation capabilities through co-expression of high-enzyme-activity alpha-amylase and glucoamylase genes. Under the conditions of clinker fermentation and raw material fermentation, the fermentation alcohol content of existing strains can be improved, addition of exogenous enzymes is reduced, cost reduction and yield increase of biological raw material ethanol can be achieved, and the large-scale industrial production requirement is met.

The invention discloses a biosynthesis method of alpha-lipoic acid, an engineering strain and a preparation method of the engineering strain. The construction of the engineering strain comprises the following steps: transforming lipocaprylamide enzyme Lpa of enterococcus faecalis, constructing the lipocaprylamide enzyme Lpa to an arabinose promoter or a Cold temperature-sensitive promoter, and then replacing the lipocaprylamide enzyme Lpa to a LacZ gene and an lpxM gene of escherichia coli through RED recombination; or the genes of LipD, lplA, lipA and lpA which are subjected to gene code modification are expressed in the saccharomyces cerevisiae through a pESC vector. The engineering strain is cultured to biologically synthesize alpha-lipoic acid, so that the fermentation cost is further reduced, cyclic synthesis is realized, and the yield is greatly increased.

The utility model provides an active ink negative pressure filtering system which comprises a filtering device and a liquid storage tank, the filtering device is communicated with the liquid storage tank, a negative pressure pipe is arranged on the liquid storage tank, the filtering device comprises a funnel, a first supporting screen plate is arranged on the funnel, the first supporting screen plate is of a net structure arranged in a wire coiling mode, and a filtering membrane can be placed on the first supporting screen plate. An electric reciprocating track is arranged on one side of the funnel and extends in the length direction of the first supporting screen plate, an ink spraying pipe is arranged on the electric reciprocating track, a spraying head is arranged at one end of the ink spraying pipe and located over the first supporting screen plate, and the ink outlet end face of the spraying head faces the first supporting screen plate. The other end of the ink spraying pipe is communicated with ink to be filtered, and the ink spraying pipe can move along the electric reciprocating track. The active ink negative pressure filtering system is high in filtering efficiency, easy to operate and high in automation degree.

This invention discloses an engineered Bacillus subtilis strain producing tagatose, its construction method, and its applications, belonging to the field of bioengineering technology. This engineered strain uses Bacillus subtilis as a host and co-expresses an L-arabinose isomerase from *Erysipelothrix larvae* and a β-galactosidase from *Escherichia coli*. The two genes are tandemly linked via ribosome binding sites or promoters to construct a Bacillus subtilis strain with dual-enzyme co-expression. This dual-enzyme Bacillus subtilis strain catalyzes the production of D-tagatose from lactose throughout the entire cell. After optimization of the enzymatic reaction, the yield and conversion efficiency of D-tagatose are significantly improved. This invention employs single-strain dual-enzyme co-expression and one-step whole-cell catalysis, using low-cost lactose as a substrate. The reaction is mild, with few byproducts, simplified process, and no endotoxin safety hazards. It solves the problems of low conversion rate, high substrate cost, and cumbersome strain formulation in existing technologies, making it suitable for the industrial production of food-grade D-tagatose.

The utility model discloses a modularized composite chemical filter, which is characterized in that a shell is provided with a through hole pair, a V-shaped frame and a buckling seat, the V-shaped frame comprises two positioning boxes which are connected to form a V shape, each positioning box is provided with a mounting port and a ventilation window, the mounting ports of the two positioning boxes in the V-shaped frame are adjacent, and the buckling seat is arranged on the V-shaped frame. Each through hole pair is located at the corresponding two adjacent mounting ports; the chemical filtering modules are arranged in the corresponding positioning boxes; the filter increasing assembly comprises a filter screen and two buckling pieces, the filter screen is located on the side, close to the through hole pair, of the V-shaped frame, and the two opposite ends of the filter screen are clamped to the two buckling structures correspondingly; the baffles penetrate through the corresponding through hole pairs and are located on the sides, close to the mounting openings, of the corresponding chemical filtering modules, and the filter screens are further clamped between the baffles and the V-shaped frame. According to the utility model, multi-stage filtration can be realized, the air filtration and purification effect is good, the disassembly, assembly, maintenance and operation are convenient, the chemical filtration module can be independently replaced, and the use cost is low.

This invention relates to the field of microbial fermentation, and particularly to a recombinant yeast strain with dual functions of saccharification and liquefaction, its construction method, and its applications. This invention provides a recombinant yeast strain with dual functions of saccharification and liquefaction. Through the co-expression of high-activity α-amylase and saccharifying enzyme genes, it exhibits superior fermentation capabilities for both cooked and raw materials. Under both cooked and raw material fermentation conditions, it can increase the fermentation alcohol content of existing strains and reduce the addition of exogenous enzymes, enabling cost reduction and increased production of ethanol from bio-based raw materials, meeting the needs of large-scale industrial production.