This invention relates to the field of microbial
fermentation, and particularly to a
recombinant yeast strain with dual functions of saccharification and
liquefaction, its construction method, and its applications. This invention provides a
recombinant yeast strain with dual functions of saccharification and
liquefaction. Through the co-expression of high-activity α-
amylase and saccharifying
enzyme genes, it exhibits superior
fermentation capabilities for both cooked and raw materials. Under both cooked and
raw material fermentation conditions, it can increase the fermentation
alcohol content of existing strains and reduce the addition of
exogenous enzymes, enabling cost reduction and increased production of
ethanol from bio-based raw materials, meeting the needs of large-scale industrial production.