Method of adding reducing agent from extraneous sources for accelerating thallus to synthesize 1,3-propylene glycol

A technology of reducing agent and propylene glycol, applied in 1 field, can solve the problems that have not been seen in reports, achieve high product synthesis concentration and conversion rate, improve bioavailability, and reduce fermentation cost

Inactive Publication Date: 2003-10-08
TSINGHUA UNIV
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

In the current literature, there are many literatures on the research on the metabolic pathway of the 1,3-propanediol biotransformation process, but the report of applying the metabolic network analysis to the actual process research has not been seen.

Method used

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Examples

Experimental program
Comparison scheme
Effect test

Embodiment 1

[0011] Embodiment 1 is with Vitamin C as reducing agent strain: Klebsiella pneumoniae (Klebsiella pneumoniae L1) medium LB solid medium: every liter of volume medium contains yeast powder 5.0g, peptone 10.0g, NaCl10.0g, Agar 15.0g,

[0012] pH7.0. Seed medium: 20g of glucose per liter volume of medium, K 2 HPO 4 .3H 2 O 4.45g, KH 2 PO 4 1.3g, (NH 4 ) 2 SO 4 2.0g,

[0013] MgSO 4 .7H 2 O 0.2g, CaCO 3 2.0g, yeast powder 1.0g, trace element solution I2.0ml, Fe 2+ Solution 1.0ml,

[0014] pH7.0. Fermentation medium: 20g of glycerol per liter volume of medium, K 2 HPO 4 .3H 2 O 4.45g, KH 2 PO 4 1.3g, (NH 4 ) 2 SO 4 2.0g,

[0015] MgSO 4 .7H 2 O 0.2g, CaCO 3 2.0g, yeast powder 1.0g, trace element solution I5.0ml, Fe 2+ Solution 1.0ml,

[0016] pH7.0. Add reducing agent Vitamin C after sterilization, make the concentration of Vitamin C in fermentation medium be 40mg / L.

[0017] Among them, the composition of trace element I (mg / L)...

Embodiment 2

[0026] Embodiment 2 is at the beginning of exponential phase (about 5 hours, OD) of thalline growth in fermentation process 650 0.5) add VC reducing agent, do not add reducing agent in fermentation medium, other conditions remain unchanged;

[0027] Program

[0028] Table 2 As can be seen from Table 2, when the concentration of VC in the fermentation broth is 40-150 mg / L, the concentration of 1,3-propanediol is finally reduced by adding VC reducing agent in the early or middle and late stages of the exponential period of bacterial growth. Increase by 2% to 25%.

Embodiment 4

[0029] Embodiment 4 is to add Vitamin E in fermentation medium

[0030] Since Vitamin E (hereinafter referred to as VE) is a fat-soluble liquid, it is difficult to dissolve in water, so the present invention dissolves VE in dimethyl sulfoxide (dimethyl sulfoxide is one of the solvents of VE, and other solvents can also be selected. Such as propylene glycol, azone, etc.), forming a 1% (v / v) solution. Take an appropriate amount and add it to the sterilized fermentation medium, so that the concentration of VE in the medium is 10 mg / L, and the rest of the steps are the same as in the above-mentioned examples. As a result of the experiment, the final concentration of 1,3-propanediol was measured to be 10.85g / L, which was 8.5% higher than that of the control group.

[0031] Program

control

Add to

10mg / L VE

Add to

20mg / L VE

Add to

40mg / L VE

Add to

60mg / L VE

1,3-propanediol

Concentration(g / L)

10

10....

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PUM

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Abstract

A process for preparing 1,3-propanediol biologically features that in the culture medium for fermenting or in the anaerobic fermenting procedure, appropriate reducer is added to increase the accumulation of reduction equivalent weight in thallus for promoting metabolism of glycerine along the reductino approach, and increasing the concentration and transform rate of 1,3-propanediol. Its advantageis high biologic utilization rate.

Description

Technical field: [0001] The invention relates to a method for adding a reducing agent from an external source to promote the synthesis of 1,3-propanediol by bacteria, belonging to the technical field of biosynthesis of 1,3-propanediol. Background technique: [0002] 1,3-propanediol (English name 1,3-propanediol, referred to as 1,3-PD) is an important chemical raw material, which can be used as an organic solvent in ink, printing and dyeing, coating, lubricant, antifreeze and other industries. [0003] The production technology of 1,3-PD is difficult and costly. At present, chemical synthesis and biosynthesis are mainly used in industry to produce 1,3-propanediol. The chemical synthesis method has the disadvantages of polluting the environment and non-renewable raw materials. Therefore, all countries are devoting themselves to the development of biosynthesis to produce 1,3-PD technology. The biosynthesis of 1,3-propanediol is a method with broad application prospects. It can...

Claims

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Application Information

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Patent Type & Authority Applications(China)
IPC IPC(8): C12P7/18
Inventor 李春张延平曹竹安
Owner TSINGHUA UNIV
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