1192 results about "Enzyme Gene" patented technology

The invention discloses a novel santalene synthase gene as well as an encoding protein and application thereof. The amino acid sequence of the santalene synthetase is as shown in SEQ ID NO. 2. The invention relates to a method for carrying out functional verification on a new sandalene synthase gene encoding enzyme from sandalwood and carrying out synthesis of sandalene and bergamoene in escherichia coli and tobacco leaves. According to the invention, the enzyme can be massively prepared by utilizing escherichia coli, is applied to synthesis of santalene and bergamoene, can be further applied to synthesis of bergamoene or santalene, and has important significance; the heterologous synthesis of the high-value santalene and the bergamoene in plants can also be realized, and the method has a wide industrial practical prospect and a large-scale development potential.

The invention belongs to the technical field of biochemical engineering and the technical field of bioengineering, and discloses a schizochytrium limacinum engineering strain for producing EPA (eicosapentaenoic acid) by prolonging a desaturation path. Part or all of the extended desaturase genes from thraustochytrium sp. And fusarium sp. And saprolegnia are expressed. According to the invention, extended desaturase genes from various sources are integrated into a schizochytrium limacinum genome, so that a schizochytrium limacinum gene engineering strain efficiently expresses the extended desaturase genes, and a heterologous EPA synthesis path with high synthesis efficiency, short culture period, simple purification method and low cost is provided; a basis is provided for producing grease rich in EPA from schizochytrium limacinum, and the problems of non-sustainability, high cost and the like caused by obtaining EPA from fishes are solved.

The invention belongs to the technical field of biology, and relates to an escherichia coli engineering strain for producing L-isoleucine through a methyl malic acid way as well as a construction method and application of the escherichia coli engineering strain. According to the invention, Escherichia coli BW25113 is used as a chassis strain, and a methyl malic acid synthase high-activity mutant gene cimA3.7, an isopropyl malic acid isomerase gene GsleuCD, a 3-isopropyl malic acid dehydrogenase gene AfleuB and a leucine dehydrogenase gene Lsleudh are over-expressed; a methyl malic acid absorption protein gene is over-expressed, and a branched chain amino acid transport system is modified to improve the exosome of the L-isoleucine; all methyl malic acid pathway related genes are integrated to a genome of a chassis strain, and a plasmid-free L-isoleucine production strain is constructed; the copy number of a key gene on a genome is optimized, and the yield of the engineering strain L-isoleucine is increased by introducing a non-oxidative glycolysis approach. The yield of the L-isoleucine after the engineering strain is fermented for 34 hours in a fermentation tank is 56.6 g / L.

The present invention relates to a recombinant yeast with high squalene production, a construction method and a use thereof, wherein the recombinant yeast is a saccharomyces cerevisiae that expresses or overexpresses HMGR gene, ADA gene, LEU2 gene, MVA pathway gene and squalene synthase gene ERG9. The present invention integrates acetaldehyde dehydrogenase gene ADA and HMG-CoA reductase gene tHMGR in multiple copies in saccharomyces cerevisiae, integrates the screened high-activity phosphoketolase gene PK and phosphotransacetylase gene PTA, weakens epoxysqualene synthesis, strengthens the mevalonate pathway, overexpresses yeast endogenous squalene synthase gene, etc. to obtain a recombinant yeast with high squalene production, so that the production in the shake flask stage of the production process exceeds 5g / L, and the production in the fed batch fermentation stage exceeds 50g / L, breaking through the current bottleneck of microbial synthesis of squalene production, and is suitable for practical promotion and application.

The invention relates to a saccharomyces cerevisiae strain for efficiently synthesizing cordycepin as well as a construction method and application of the saccharomyces cerevisiae strain, and belongs to the technical field of microbial genetic engineering. The construction method provided by the invention comprises the following steps: carrying out multi-copy genome integrated expression on heterologous codon optimized 2 '-carbonyl-3'-deoxyadenosine reductase genes and 3 '-adenosine monophosphate phosphohydrolase genes, so as to increase the dosage of key enzymes; and supplementing the defective gene of the engineering strain to obtain the saccharomyces cerevisiae strain B5U3 with high yield of cordycepin. According to the obtained saccharomyces cerevisiae strain for efficiently synthesizing the cordycepin, the cordycepin yield reaches 2.92 g / L after shake flask fermentation, the cordycepin yield reaches 7.5 g / L after fermentation in a fermentation tank, and a foundation is laid for safer and more reliable industrial production of the cordycepin.

The present invention relates to the construction of a high-yield indigo genetic engineering strain and a fermentation method. The present invention uses Escherichia coli as a starting strain, knocks out at least one of the yddG gene, trpR gene, tyrA gene or pheA gene in the host cell; integrates the tryptophanase gene tnaA and / or the flavin monooxygenase gene MaFMO on the genome; integrates one or more genes related to biofilm modification on the genome, and obtains the optimal engineering strain by comparing the fermentation yield; then transfers a plasmid into the strain to further strengthen the tryptophanase gene tnaA and the flavin monooxygenase gene MaFMO; finally, the yield is significantly increased by supplementing carbon sources and substrates and adding surfactants. The present invention provides an indigo-producing genetic engineering strain and a fermentation method with good stability and high synthesis efficiency, providing a reference for the subsequent development of more advantageous engineering strains.

The invention discloses an attenuated salmonella typhimurium EN-VNP (Enhanced Natriuretic Peptide) strain as well as a preparation method and application of the attenuated salmonella typhimurium EN-VNP strain. Comprising the following steps: on the basis of a salmonella VNP20009 strain, flagellin genes fliC and fljB, a phosphoethanolamine transferase gene eptA, a 4-amino-4-deoxy-L-arabinose transferase gene arnT and phosphotransferase yeiU are sequentially knocked out or silenced, and a strain is formed after a phosphotransferase gene lpxE is knocked in or overexpressed, so that the attenuated salmonella typhimurium EN-VNP strain is prepared. According to the attenuated salmonella typhimurium EN-VNP with low toxicity and the T cell adjuvant effect and an EN-OMV antigen delivery system prepared from the attenuated salmonella typhimurium EN-VNP, an improved strain does not contain flagellin, an LPS molecular structure is changed, the attenuated salmonella typhimurium EN-VNP has the low toxicity and the T cell adjuvant effect, and normal multiplication culture can be achieved.

The invention relates to the technical field of biological enzymes. The invention provides a biological enzyme gene capable of producing beta-pinene and application of the biological enzyme gene. The biological enzyme gene comprises one or more of TPS1, TPS2, TPS3 and TPS4. The gene disclosed by the invention can be used for preparing a biological enzyme for efficiently catalyzing and producing beta-pinene, and the biological enzyme is used as a catalyst, so that the yield of beta-pinene can be increased, and an efficient, environment-friendly and sustainable production mode is realized. The method can become an important production way of beta-pinene in the future, and has a wide application prospect in industrial production and other fields.

The invention belongs to the technical field of genetic engineering, and discloses a Yarrowia lipolytica engineering strain capable of efficiently producing eicosapentaenoic acid through a cofactor strategy, Yarrowia lipolytica Po1f is used as an original strain, and genes of delta 9 elongase, delta 8 desaturase, delta 5 desaturase and delta 17 desaturase are over-expressed, so that the eicosapentaenoic acid can be efficiently produced. And simultaneously overexpressing glucose-6-phosphate dehydrogenase (G6PDH) and malic enzyme (ME) to obtain the gene. The construction method is suitable for constructing a genetic engineering strain for producing eicosapentaenoic acid, the aim of simply, conveniently, effectively and accurately constructing the strain is achieved, the constructed engineering strain is further applied to production of eicosapentaenoic acid, and an effective way is provided for commercially producing eicosapentaenoic acid by applying yarrowia lipolytica.

Described are RNAi agents, compositions that include RNAi agents, and methods for dual inhibition of an Apolipoprotein C-III (APOC3) and Proprotein Convertase Subtilisin Kexin 9 (PCSK9) gene. The APOC3-PCSK9 RNAi agents disclosed herein inhibit the expression of an APOC3 and a PCSK9 gene. Pharmaceutical compositions that include one or more APOC3-PCSK9 RNAi agents, optionally with one or more additional therapeutics, are also described. Delivery of the described TSLP RNAi agents to hepatic cells, in vivo, provides for inhibition of APOC3 and / or PCSK9 gene expression, which can provide a therapeutic benefit to subjects, including human subjects, for the treatment of various diseases including hypertriglyceridemia and hypercholesterolemia.

The invention discloses a schizochytrium limacinum genetic engineering strain capable of remodeling an FAS (Fibroblast Activated Sequence) pathway as well as a construction method and application thereof. The schizochytrium limacinum gene engineering strain is obtained by heterologous expression of a C16 elongase gene E16, a C18 elongase gene E18, a delta 9 desaturase gene D9, a delta 5 desaturase gene D5, a delta 6 desaturase gene D6 and a delta 15 desaturase gene D15 in a schizochytrium limacinum wild type. The recombinant schizochytrium limacinum described herein is capable of producing EPA comprising 2.08% by total fatty acids while also producing DHA comprising at least more than 41% by total fatty acids. In addition, the invention discloses a method for producing unsaturated fatty acid through a double-path channel of PKS and FAS, which is beneficial to promotion and application of a technology for producing EPA and DHA by schizochytrium limacinum.

The invention relates to the technical field of microbial genetic engineering, in particular to a method for improving cordycepin synthesized by saccharomyces cerevisiae and saccharomyces cerevisiae engineering bacteria. According to the invention, codon-optimized 2 '-carbonyl-3'-deoxyadenosine reductase gene and 3 '-adenosine monophosphate phosphohydrolase gene from cordyceps militaris are expressed by using plasmids, and then the plasmids are transformed into host cells to obtain the saccharomyces cerevisiae engineering bacteria. And inoculating a seed solution obtained by seed culture of the activated saccharomyces cerevisiae engineering bacteria into a fermentation culture medium containing a metabolic effector, and carrying out fermentation culture to synthesize cordycepin. Metabolic effectors (Cu < 2 + >, Fe < 2 + >, Mg < 2 + >, Zn < 2 + >, citric acid, cysteine, aspartic acid, glycine, VB1 and tea polyphenol) are added into a fermentation culture medium strain, and the variety and concentration of the metabolic effectors are optimized, so that the yield and synthesis efficiency of cordycepin are remarkably improved. By using the combination of the strain obtained by the invention and the optimal effector, the yield of cordycepin in a fermentation tank reaches 2.9 g / L.

The invention provides a protein for synthesizing an octadecene compound and a method for synthesizing the octadecene compound. Relates to the field of enzymology. The amino acid sequence of the protein has more than 95% of consistency with the sequence as shown in SEQ ID No.3. The protein, hexadecanoic acid specific thioesterase, Z11 desaturase, gene silencing suppressor and vegetable fat synthesis related transcription factors are jointly expressed in plants and can be used for synthesizing (Z)-13-octadecenoic acid.

The invention discloses SGNH family esterase SH2 screened in a Tibetan hot spring environment and application of the SGNH family esterase SH2, and belongs to the technical field of gene engineering. According to the invention, a new SGNH family esterase gene is screened from a Tibetan hot spring environment, and recombinant expression is realized in escherichia coli. The recombinant esterase prepared by the invention has the characteristics of thermal stability, salt resistance, organic solvent resistance and the like, can be safely applied to tooth whitening or skin exfoliating products, avoids damage of traditional chemical bleaching agents to enamel, and has important application value in the fields of daily chemicals, medicines, food processing and the like.

The invention discloses a genetically engineered bacterium for producing uridine diphosphate glucose as well as a construction method and application of the genetically engineered bacterium, and belongs to the technical field of genetic engineering. The following genes are overexpressed in the genetically engineered bacterium: a pyrimidine nucleoside operon gene, a carbamyl phosphate synthetase gene, an aspartate carbamyl transaminase gene, an orotic acid phosphoribose transferase gene, a phosphoribose pyrophosphate synthase gene, a uridylate kinase gene and a glucophosphate mutase gene. A uridine triphosphate glucose-1-phosphate uridine group transferase gene; the genetically engineered bacterium does not express the following genes: a purine nucleotide repressor protein gene, a deoxyribonucleoside triphosphate reductase gene, a nucleoside triphosphate pyrophosphohydrolase gene, a cytidine triphosphate synthase gene, a pyrimidine nucleotidase gene, a nucleotidase gene, an ornithine carbamoyltransferase gene, a UDPG dehydrogenase gene and a lacI repressor protein gene. The genetically engineered bacterium can be used for efficiently synthesizing UDPG by taking glucose as a substrate.

The invention discloses recombinant I-type collagen with a low-temperature self-assembly characteristic as well as a preparation method and application thereof, and relates to the technical field of genetic engineering. The recombinant I-type collagen with the low-temperature self-assembly characteristic is characterized in that an amino acid sequence of the recombinant I-type collagen is obtained by taking a human I-type collagen fragment as a basic unit and repeatedly connecting in series for n times, an amino acid sequence of the human I-type collagen fragment is shown as Seq ID NO.1, and n is an integer greater than or equal to 1. According to the invention, nucleotides of repetitive fragments of functional areas of the human-derived I-type collagen are optimized and screened, and co-expressed with hydroxyproline enzyme genes in a pichia pastoris system, so that the recombinant I-type collagen with low-temperature self-assembly characteristics is successfully obtained.

The invention discloses a trichoderma reesei engineering bacterium for secreting and expressing neutral cellulase and a construction method of the trichoderma reesei engineering bacterium, and belongs to the technical field of genetic engineering. In a trichoderma reesei engineering bacterium, two endogenous acidic cellulase genes cbh1 and cbh2 in the trichoderma reesei are sequentially replaced by genes for coding neutral cellulase, and the trichoderma reesei is converted into the engineering bacterium for secreting and expressing the neutral cellulase; the amino acid sequence of neutral cellulose is as shown in SEQ ID NO.1, and the nucleotide sequence of the coding gene is as shown in SEQ ID NO.2. The trichoderma reesei engineering strain for secreting and expressing the neutral cellulase is successfully constructed and has a wide pH application range, and a new strain and method are provided for production of the neutral cellulase in the fields of textile and the like.

The invention relates to the technical production field of metabolic engineering and genetic engineering, and particularly provides a uracil production strain as well as a construction method and application thereof. According to the strain, a uridine kinase gene udk is knocked out on an E.coli W3110 genome, and meanwhile, a T7RNA polymerase gene is integrated; the uridine monophosphate kinase gene pyrH is weakened; the method comprises the following steps: overexpressing a carbamyl phosphate synthetase gene carAB, a ribose phosphate pyrophosphate kinase gene prsA, a pyrimidine-5 '-nucleoside enzyme gene ppnN, UMP phosphohydrolase genes umpH and umpG, a ribonucleoside hydrolase 1 gene rihA and a glutamine synthetase gene glnA; an orotic acid nucleoside monophosphate decarboxylase gene pyrF and an orotic acid ribosyltransferase gene pyrE of wild type bacillus subtilis B.subtilis 168 are introduced in a heterologous manner, so that an orotate nucleoside monophosphate decarboxylase gene pyrF and an orotate ribosyltransferase gene pyrE are obtained. The safe, efficient and low-cost production of the uracil is realized.

The invention relates to screening and application of a Candida antarctica lipase B mutant. Specifically, the Candida antarctica lipase B mutant provided by the invention is obtained by carrying out rational design and directed evolution combined strategy mutation on the basis of a wild Candida antarctica lipase B gene sequence, and the amino acid sequence of the mutant is shown as SEQ ID NO.3. The invention also relates to a preparation method of the Candida antarctica lipase B mutant. The mutant has higher enzyme activity and thermal stability, the hydrolytic catalytic activity of the mutant is improved to 2.1 times of the original hydrolytic catalytic activity, and the half-life period of the mutant enzyme at 50 DEG C is prolonged by 4 times. On the basis, pichia pastoris is used as a host to express the mutant, and a strain with hydrolytic activity improved by 52% is obtained through high resistance screening. The lipase is prepared into an immobilized enzyme to be used in a synthetic reaction of chlorogenic acid derivatives, the highest conversion rate of chlorogenic acid reaches 74% in 24 hours, and the immobilized enzyme has a good industrial application prospect.

The invention discloses application of an OfTPS7 gene in synthesis of an important terpenoid aroma substance linalool and an oxide thereof, a plant overexpression vector containing the OfTPS7 gene is used for instantaneously converting OfTPS7 gene into OfTPS7 petals and detecting volatile matters of infected petals, compared with a control group, the expression quantity of OfTPS7 is increased by 3.13 times, the content of linalool is increased from 182.25 mu g / gFW to 1851.12 mu g / gFW, and the content of linalool is increased by 3.12 mu g / gFW. And the content of linalool oxide is increased from 452.09 [mu] g / gFW to 3072.41 [mu] g / gFW. The OfTPS7 gene overexpressed in the petals can significantly improve the content of linalool and oxides thereof in the petals, which proves that the OfTPS7 is the linalool synthetase gene and can be used for improving the natural aroma quality of osmanthus fragrans, breeding excellent varieties and the like in osmanthus fragrans genetic engineering.

The invention discloses a genetically engineered bacterium and an application of the genetically engineered bacterium in production of cis-3-hydroxy-L-proline. According to the genetically engineered bacterium, a proline dehydrogenase gene putA and a proline transporter gene putP in a starting bacterium are silenced, and meanwhile, a gamma-glutamyl kinase mutant gene proBD107N, a gamma-glutamyl phosphate reductase gene proA and a cis-3-hydroxy-L-proline hydroxylase gene P3H are subjected to overexpression. And by comparing a plurality of common escherichia coli, the cis-3-hydroxy-L-proline hydroxylase gene P3H and different gamma-glutamyl kinase mutant genes, the highest yield combination is preferably selected. The strain takes glucose as a raw material, so that the production cost is greatly saved, and the strain has a relatively good industrial utilization value.

The invention discloses a phoebe zhennan PzGGPS12 gene and application of the phoebe zhennan PzGGPS12 gene to improvement of yield and drought tolerance of plant terpenoids, and belongs to the field of genetic engineering, a key enzyme gene PzGGPS12 for catalyzing biosynthesis of the terpenoids in phoebe zhennan wood is screened and analyzed, and a full-length CDS nucleic acid sequence of the PzGGPS12 is obtained. Transgenic experiments prove that overexpression of the gene improves the relative content of tobacco monoterpenes and triterpenes. Short-term drought experiments prove that overexpression of the gene can promote biosynthesis of sesquiterpenes, diterpenes and triterpenes so as to enhance drought tolerance of transgenic tobacco. Therefore, the gene can be introduced into a plant as a target gene, the yield of terpenoids in the plant is increased, and the drought tolerance of the plant is enhanced so as to improve the variety of the plant.

The invention discloses a rare ginsenoside dammadienol synthetase and an application of the rare ginsenoside dammadienol synthetase in preparation of dammadienol. The dammadienol synthetase is identified for the first time in the research, a metabolic network of a saccharomyces cerevisiae host cell is modified through a metabolic engineering strategy, genes which do not exist in the host cell are introduced into an engineering strain for heterologous expression, the host cell is changed from nothing to existence, and the dammadienol engineering strain is constructed. In addition, through homologous modeling, key catalytic pocket searching and key enzyme gene site-directed mutagenesis modification, enzyme catalysis efficiency is improved, efficient synthesis of dammadienol is achieved, the problem that natural sources of the rare ginsenoside are insufficient is solved, green synthesis and sustainable utilization of the rare ginsenoside are promoted, and the method is suitable for industrial production. And a foundation is laid for subsequent construction of rare ginsenoside cell factories.

The invention discloses a self-assembly enzyme for directional synthesis of uniform low molecular weight dextran, the self-assembly enzyme is named as YG-SpyTag-SpyCatcher-padex, the YG is a dextran sucrase gene (Genbank No.DQ345760), the padex is a dextran enzyme gene (Genbank No.KF999646.1), the SpyTag and the SpyCatcher are two specific oligopeptides, and aspartic acid in the SpyTag and lysine in the SpyCatcher spontaneously form an isopeptide bond. The invention belongs to the technical field of biology, and relates to an enzyme engineering technology. According to the invention, dextran sucrase derived from an escherichia coli genetically engineered bacterium BL21 (DE3)-YG-SpyTag and dextran enzyme derived from a pichia pastoris genetically engineered bacterium X33-SpyCatcher-padex are combined through a SpyTag-SpyCatcher protein covalent coupling system, and the obtained self-assembled enzyme can be used for catalyzing the substrate sucrose to be directly converted into dextran with the molecular weight of 3000 to 4000 Da.

The invention discloses a recombinant strain for producing squalene as well as a construction method and application of the recombinant strain. The construction method comprises the following steps: heterologously expressing cDNA (complementary deoxyribonucleic acid) of each gene in a squalene synthesis route from filamentous fungi in yeast; the squalene synthetic pathway genes comprise a mevalonic acid-5-phosphate kinase gene, a squalene synthase gene, an acetoacetyl coenzyme A thiolase gene, a mevalonic acid kinase gene, a hydroxymethyl glutaryl coenzyme a synthetase gene, a mevalonic acid pyrophosphate decarboxylase gene, a farnesyl pyrophosphate synthetase gene and isopentenyl pyrophosphate. A dimethallylpyrophosphate isomerase gene and a truncated hydroxymethyl glutaryl coenzyme a reductase gene; the culture medium is characterized in that the filamentous fungi are Saroocladium oryzae or Stibella sp. 1056, and the culture medium is a culture medium. The excavated squalene synthesis route can complete efficient de novo synthesis of squalene, and the squalene yield is increased.

The invention discloses application of programmable TnpB nuclease gene editing, and relates to the technical field of gene editing. The TnpB nuclease is any one of (A1), (A2) and (A3): (A1) TnpB nuclease, the amino acid sequence of which is as shown in SEQ ID NO.1; (A2) carrying out substitution, deletion and / or addition of one or more amino acid residues on the amino acid sequence of the TnpB nuclease to obtain a protein which has 80% or more of identity with the TnpB nuclease and has the same biological function with the TnpB nuclease; and (A3) a fusion protein obtained by connecting a protein tag to the N terminal or / and C terminal of (A1) or (A2). The TnpB nuclease provided by the invention has cis-cleavage activity and trans-cleavage activity, and has a wide application prospect in the fields of nucleic acid detection and gene editing.

The invention discloses a genetically engineered bacterium for producing glucuronic acid as well as a construction method and application of the genetically engineered bacterium, and belongs to the technical field of genetic engineering. According to the genetically engineered bacterium for producing glucuronic acid, an inositol oxidase gene MMIOX is integrated on a site of a uronic acid isomerase gene uxaC. According to the invention, an inositol oxidase gene MMIOX is integrated on a genome of a genetically engineered bacterium for producing glucuronic acid, and a uronic acid isomerase gene uxaC is knocked out at the same time, so that the genetically engineered bacterium can efficiently catalyze inositol reaction to generate glucuronic acid through the integrated inositol oxidase gene MMIOX; the glucuronic acid isomerase gene uxaC is knocked out, so that isomerization reaction of glucuronic acid can be avoided, and the yield of glucuronic acid is further increased.

The invention belongs to the technical field of biology, and discloses a yarrowia lipolytica engineering strain for efficiently producing eicosapentaenoic acid based on citric acid metabolism regulation, a method and application of the yarrowia lipolytica engineering strain. Delta9 elongase, Delta8 desaturase, Delta5 desaturase and Delta17 desaturase genes in a fatty acid synthesis route, heterologous ATP-citrate lyase and a mitochondrial citric acid vector are overexpressed. According to the invention, ATP-citric acid lyase and a mitochondrial citric acid carrier gene which are respectively derived from Schizochylene sp. And Saccharomyces cerevisiae are jointly expressed in the yarrowia lipolytica engineering strain, so that the yields of EPA and grease in the yarrowia lipolytica engineering strain are remarkably improved, and compared with a control strain, the yields of the grease and the EPA are respectively improved by 243.35% and 161.85%.

The invention discloses bacillus subtilis engineering bacteria capable of efficiently producing pullulanase and application of the bacillus subtilis engineering bacteria in trehalose enzyme method production, and belongs to the technical field of genetic engineering. The invention discloses a bacillus subtilis engineering bacterium capable of efficiently producing pullulanase. The bacillus subtilis engineering bacterium comprises a pullulanase gene of which the nucleotide sequence is as shown in SEQ ID NO.7. According to the invention, the bacillus subtilis strain is used as an expression host, and the bacillus subtilis is modified through an engineering technical means, so that the pullulanase is highly expressed, and the fermentation cost of pullulanase liquid in the production process of trehalose is reduced.

The invention belongs to the technical field of gene engineering, and relates to high-temperature-resistant laccase, a gene and application of the high-temperature-resistant laccase in mycotoxin degradation. The amino acid sequence of the laccase TtLac-4 is as shown in SEQ ID NO: 1. On the basis of wild laccase, site-directed mutagenesis modification is carried out through artificial rational design, and an amino acid sequence of the modified high-temperature-resistant laccase and a nucleotide sequence of the artificially optimized high-temperature-resistant laccase gene are obtained. The high-temperature-resistant laccase obtained through gene coding of the high-temperature-resistant laccase has the remarkably-improved high-temperature-resistant characteristic, and the optimum temperature reaches 90 DEG C. The degradation rate of the laccase TtLac-4 obtained by the invention on mycotoxin aflatoxin B1 and zearalenone reaches 100%. The invention lays a foundation for a green degradation process and application of mycotoxin degradation.