3 results about "Lipase b" patented technology

The application discloses an enzymatic production process of diglyceride, and comprises the following steps: using low-cost lipase as a catalyst, preparing lipase A through immobilization of the enzyme, preparing lipase B through an ester exchange reaction, and then using the lipase B as a catalyst to catalyze esterification of free fatty acid and glycerol to produce diglyceride; the method can realize efficient enzymatic production of diglyceride through a series of methods by using low-cost lipase, and the production cost is reduced. The application provides an economical and efficient method for producing diglyceride by using the enzyme method, greatly reduces the use cost of lipase in the enzymatic esterification reaction, and has certain industrialization application prospect.

The present application relates to the field of genetic engineering and bioengineering technology, in particular to a Candida antarctica lipase B mutant and application thereof. The Candida antarctica lipase B mutant provided by the present application is obtained by joint mutation of the 143th and 284th positions in the amino acid sequence shown in the wild type lipase B. The Candida antarctica lipase B mutant constructed by the present application has 12.5 times higher enzyme activity than the wild type enzyme, and the protein expression amount is as high as 1.8-1.9 g / L under the scale of 5 L fermenter, which can significantly improve the yield and reduce the production cost. In addition, the Candida antarctica lipase B mutant can be used for synthesis of high value-added, non-natural substrates such as vitamin C fatty acid ester and vitamin A oleate, which lays a foundation for the application of lipase in the field of fine chemical industry.

The present application relates to the technical field of oil and fat chemistry, and particularly relates to a preparation method and application of a diglyceride oil with the function of improving cardiovascular and cerebrovascular health. The preparation method of the diglyceride oil comprises the following steps: (1) mixing oil and water, adding a first hydrolytic enzyme to perform first hydrolysis, and then adding a second hydrolytic enzyme to perform second hydrolysis, to obtain a hydrolysis product; (2) adding the hydrolysis product to glycerol and a lipase to perform reaction, and then distilling, to obtain the diglyceride oil; in step (1), the first hydrolytic enzyme is Lipozyme RM IM, and the second hydrolytic enzyme is Candida antarctica lipase B; in step (2), the lipase is a mixture of lipase Lipase G50 and Rhizomucor niveus lipase, and the mass ratio of the two is 1:1-3. The diglyceride oil prepared by the method has the function of improving cardiovascular and cerebrovascular health.