The invention a bletilla striata differentiation and seedling strengthening medium and relates to the technical field of bletilla striata planting and cultivation. The bletilla striata differentiation and seedling strengthening medium is prepared from the following raw materials including potassium nitrate, 440-490mg of ammonium sulfate, monopotassium phosphate, magnesium sulfate, calcium chloride, manganese sulfate, zinc sulfate, boric acid, potassium iodide, sodium molybdate, copper sulfate, cobalt chloride, ferrous sulfate, EDTA (Ethylene Diamine Tetraacetic Acid), phaseomannite, thiamine sulfate, pyridoxine sulfate, nicotinic acid, glycine, DMPT, sodium para-nitrophenolate, sodium ortho-nitrophenol, 5-nitroguaiacol sodium salt, 6-BA, NAA, PP-333, CPPU, citric acid, and cane sugar under a proper pH value condition. The bletilla striata differentiation and seedling strengthening medium can improve the differentiation effects of bletilla striata seeds, and promotes the growth of seedlings and the fast formation of pseudobulb, so that the germination rate of the seeds can be effectively increased, and the growth speed of an embryoid can be increased.