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Preparation method of molecular imprinting colloid array and application thereof

A technology of molecular imprinting and colloid, which is applied in the fields of applied chemistry and clinical analysis and testing, can solve the problems of photonic crystal templates being easily destroyed and complicated preparation methods, and achieve the effects of shortening the preparation time, simplifying the preparation process, and strong mechanical properties

Inactive Publication Date: 2012-02-15
BEIJING INSTITUTE OF TECHNOLOGYGY
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  • Abstract
  • Description
  • Claims
  • Application Information

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Problems solved by technology

[0006] In view of the defects that the current preparation methods of molecularly imprinted photonic crystals are complex and the templates of photonic crystals are easily destroyed, one of the purposes of the present invention is to provide a method for preparing molecularly imprinted colloidal arrays, which is more advanced than the preparation method of molecularly imprinted photonic crystals. Simple, the prepared molecularly imprinted colloidal array is a molecular recognition optical sensing material that takes into account both the performance of selection and signal recognition

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  • Preparation method of molecular imprinting colloid array and application thereof
  • Preparation method of molecular imprinting colloid array and application thereof
  • Preparation method of molecular imprinting colloid array and application thereof

Examples

Experimental program
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Effect test

Embodiment 1

[0044] (1) Preparation of estrone molecularly imprinted colloidal microspheres

[0045] Under nitrogen protection, add 255 mL of distilled water into a 1000 mL four-necked flask, then add 24 mL of methyl methacrylate dissolved in 0.3 mmol estrone and 6 mmol acrylamide into the four-necked flask, mix and stir, heat to 70 °C, and then Add 15mL of 0.018g / ml potassium peroxodisulfate aqueous solution, react until the reaction solution becomes a white emulsion to stop the reaction, and obtain a colloidal microsphere solution with estrone molecular imprinting; divide the colloidal microsphere solution with estrone molecular imprinting Put the device in a 15mL centrifuge tube, centrifuge at 4000r / min for 4min, remove the supernatant, then add 3mL of acetic acid / methanol (2 / 8, v / v) solution to wash, then place it on a shaker for 30min, and wash as above Methods Repeated centrifugation and washing three times; then washed three times with methanol solution, and finally washed three tim...

Embodiment 2

[0051] (1) Preparation of estrone molecularly imprinted colloidal microspheres

[0052] Under nitrogen protection, add 225 mL of distilled water into a 1000 mL four-necked flask, then add 30 mL of methyl methacrylate dissolved in 0.6 mmol estrone and 3 mmol acrylamide into the four-necked flask, mix and stir, heat to 80 °C, and then Add 15mL of 0.054g / mL potassium peroxodisulfate aqueous solution, react until the reaction solution becomes a white emulsion to stop the reaction, and obtain a colloidal microsphere solution with estrone molecular imprinting; divide the colloidal microsphere solution with estrone molecular imprinting Put the device in a 15mL centrifuge tube, centrifuge at 4000r / min for 4min, remove the supernatant, then add 3mL of acetic acid / methanol (2 / 8, v / v) solution to wash, then place it on a shaker for 30min, and wash as above Methods Repeated centrifugation and washing 4 times; then washed 4 times with methanol solution, and finally washed 4 times with dist...

Embodiment 3

[0058] (1) Preparation of estrone molecularly imprinted colloidal microspheres

[0059] Under nitrogen protection, add 200 mL of distilled water into a 1000 mL four-necked flask, then add 12 mL of methyl methacrylate dissolved in 1 mmol estrone and 10 mmol acrylamide into the four-necked flask, mix and stir, heat to 80 °C, and then add 15mL of 0.03g / mL potassium peroxodisulfate aqueous solution, react until the reaction solution becomes a white emulsion to stop the reaction, and obtain a colloidal microsphere solution with estrone molecular imprinting; divide the colloidal microsphere solution with estrone molecular imprinting In a 15mL centrifuge tube, centrifuge at a speed of 4000r / min for 4min, remove the supernatant, then add 3mL of acetic acid / methanol (1 / 9, v / v) solution to wash, and then place it on a shaker for 30min, follow the above washing method Repeated centrifugal washing for 5 times; then washing with methanol solution for 5 times, and finally washing with disti...

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Abstract

The invention relates to a preparation method of a molecular imprinting colloid array and an application thereof, and belongs to the technical field of applied chemistry and clinical analysis and test. By the combination of molecular imprinting and colloid array technologies, the preparation method comprises the following steps of: respectively using oestrone, beta-estradiol, p-nitrophenol and melamine as template molecules, preparing template molecular imprinting colloid microballoons, followed by direct self-assembly and alignment to obtain the corresponding molecular imprinting colloid array. The method provided by the invention overcomes defects that a preparation method of molecular imprinting photonic crystals is complex and the photonic crystal template is easy to destroy; and by the adoption of the method, the preparation technology is simplified and the preparation time is shortened. The prepared molecular imprinting colloid array has adsorption selectivity and obvious optical performance response for the template molecules, and can be used to provide a simple effective method for preparing optical sensor materials and provide the possibility for ''naked eye detection''.

Description

technical field [0001] The present invention relates to a preparation method and application of a molecularly imprinted colloidal array, in particular to a preparation method of a new type of optical sensing material-molecularly imprinted colloidal array (Molecularly imprinted crystallinearray, MICA); also provides the The application of the molecularly imprinted colloidal array, that is, the molecularly imprinted colloidal array is respectively applied to the detection of estrone, β-estradiol, p-nitrophenol and melamine; it belongs to the technical field of applied chemistry and clinical analysis and testing. Background technique [0002] Endocrine Disrupting Chemicals (EDCs), also known as environmental hormones, are exogenous chemical substances that interfere with the endocrine system. Such substances can cause animal and human genital disorders, abnormal behavior, decreased reproductive ability, larval death or even extinction. Estrone, β-estradiol and p-nitrophenol bel...

Claims

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Application Information

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Patent Type & Authority Applications(China)
IPC IPC(8): B01J20/26B01J20/28B01J20/30C08F220/14C08F220/56C08J9/26
Inventor 孟子晖薛飞王一飞刘烽黄舒悦王秋鸿
Owner BEIJING INSTITUTE OF TECHNOLOGYGY
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