218results about How to "Strong differentiation ability" patented technology

A neutron detector utilizing a sol-gel absorber incorporating a fissionable material and an activation disk. Preferably utilizing Li-6 and B-10 as fissionable material and Ag-109 as activation disk material for increased sensitivity and better differentiation of thermal versus prompt neutrons and neutrons versus other radiation fragments.

The invention discloses a preparation method and application of an antibacterial bio-ceramic film with the titanium or titanium alloy surface containing copper and belongs to the technical field of treating metal surfaces. The preparation method comprises the following steps: preparing a Ca, P and Cu containing porous hard ceramic layer under a single square wave pulsed power supply mode; and carrying out hydrothermal treatment or alkali treatment to obtain the CuO / HA antibacterial bio-ceramic film. According to the preparation method disclosed by the invention, the prepared ceramic layer and a matrix are in metallurgical bonding and are good in bioactivity and obvious in antibacterial effect; the prepared antibacterial bio-ceramic film is suitable for dental implant in the field of oral cavities as well as bone union plates, bone nails and the like of orthopaedics; and the ceramic layer is good in protein adsorbability and adhesion, increment and differentiation abilities of cells and is capable of greatly shortening the bonding time of the traditional medical implanting material and bones.

The invention discloses a preparation method of a CAR-T cell for treating AIDS-related lymphoma. A CD8+ T cell is used to produce the CAR-T cell, and the CD8-T cell derives from a cord blood T cell. The method includes the following steps: preparing cord blood mononuclear cells from cord blood, removing tumors and other cells by using a human T cell purification kit to obtain T cells, and carryingout separation by using magnetic beads to obtain the CD8+ T cell; activating the CD8+ T cell by using an appropriate medium and appropriate stimulation conditions; transferring CAR to the CD8+ T cellby using lentivirus to prepare the CAR-T cell; and amplifying the CAR-T cell in vitro by using cytokines and activating stimulators to achieve the desired effective dose. The invention also relates to the CAR-T cell prepared by the method, and an application thereof. The CAR-T cell prepared in the invention has a good cell activity and a high proliferation speed, and reduces the attack risk of GVHD; and only the CD8+T cell of the umbilical blood T cells of AIDS patients is used to prepare the CAR-T cell for, so the risk of input CAR-T infected with in-vivo HIV is reduced.

The invention discloses a stem cell medium and an application thereof. The medium comprises a basic medium, fetal bovine serum, cytokine or protein polypeptide, vitamin and lipid. The medium provided by the invention can quickly amplify stem cells and does not influence the potential of the stem cells, and the amplification velocity of the stem cells is improved by 3-5 times than a conventional medium; and moreover, the medium can be used for culturing the stem cells of various tissues and has excellent applicability, the cultured stem cells have strong differentiation capability and can be differentiated into cells with various functions; therefore, the stem cell medium has high scientific and research and medical application values.

The guide objects 40L and 40R instruct the movements of the respective left and right hands. The image sensor 54 captures images of the input instruments 3L and 3R which are worn on left and right hands respectively and the cursors 70L and 70R are connected with the movements of the input instruments 3L and 3R by processing the result of the imaging. A series of the processing and transmission of information in the order of the eyes, the visual nerve, the brain, the motor nerve, and the hands and arms are performed inside of a human body, which are not performed in a normal life, by making the operator perform the independent motions of the respective left and right hands, which are not performed in a normal life.

The invention relates to a reprogrammed culture medium and a culture method of a reprogrammed induced multipotential stem cell. The reprogrammed culture medium is composed of a DMEM-F12 basal culturemedium and additive components, wherein the additive components comprise 60-180 microgram / mL of L-ascorbic acid, 5.3-74 micromole / L of hydrocortisone, 3-89 nanogram / mL of sodium selenite, 8-23 micromole / L of Optiferrin, 0.5-7.4 micromole / L of retinyl acetate, 40-60 nanogram / mL plant-derived recombinant human alkaline growth factors, 8-12 microgram / mL of IGF, 0.2-0.6 microgram / mL A-83, 2-6 micromole / L of CHIR99021 and 100-450 micromole / L of sodium butyrate. The use of the reprogrammed culture medium not only can improve the safety of clinical use, but also can improve the efficiency of adult cell reprogramming to induce the multipotential stem cell.

Disclosed herein are methods and compositions for replenishing injured and / or damaged cardiomyocytes in a subject by inducing, increasing, and / or enhancing the differentiation of endogenous stem and progenitor cells in the subject.

The invention discloses a stem cell culture medium. The stem cell culture medium comprises an improved DMEM (Dulbecco's Modified Eagle Medium) / F12 basal culture medium and an additive, wherein the additive comprises sodium bicarbonate, selenium amino acid chelate, recombinant human insulin growth factors, recombinant human basic fibroblast growth factors, recombinant human lactoferrin, ascorbic acid, recombinant human platelet-derived growth factors, recombinant human vascular endothelial cell growth factors, octacosanol, polyvinyl alcohol, polyvinylpyrrolidone and recombinant human epidermal growth factors. According to the stem cell culture medium, the potential of stem cells is not influenced while the stem cells can be proliferated rapidly, the proliferation speed of the stem cells is increased by 3-5 times compared with a common culture medium, and further, the stem cell culture medium can be used for culturing the stem cells of various kinds of tissue and has excellent applicability; the cultured stem cells have high differentiation capability, can be differentiated into multiple functional cells and have very high scientific research and medical application values, culture medium components are exact, the quality is stable, and accordingly, the cultured stem cells are not likely to generate human body rejection reaction after transplanting.

The invention discloses a method for breeding chamomile. The method disclosed by the invention comprises the following steps: (1) inoculating chamomile seeds onto a seed germination culture medium to carry out sterile seedling cultivation so as to produce sterile seedlings; (2) taking hypocotyls of the sterile seedlings as explants, inoculating the explants onto a callus induction culture medium to carry out callus induction culture so as to generate calluses by virtue of induction; (3) inoculating the calluses generated by induction onto an adventitious bud differentiation culture medium to carry out adventitious bud induction differentiation culture so as to obtain adventitious buds; (4) inoculating the adventitious buds onto a rooting culture medium to carry out rooting culture and culturing adventitious roots by virtue of induction so as to obtain rooted seedlings; and (5) hardening and transplanting the rooted seedlings so as to finally obtain the chamomile. In a regeneration system established by the method disclosed by the invention, callus inductivity reaches up to 86.63%, differentiation rate of the adventitious buds reaches up to 25.5%, rooting rate reaches up to 100%, and transplanting survival rate reaches up to 100%, thus a large number of excellent chamomile test-tube plantlets can be obtained in short term so as to realize large-scale factory production.

The invention discloses a culture method for restoring totipotency of a mesenchymal stem cell. The method for restoring totipotency of a mesenchymal stem cell, provided by the invention, comprises the following steps: 1) performing hanging-drop culture on an in-vitro mesenchymal stem cell with reduced totipotency, thus obtaining a hanging-drop cultured cell; and 2) further performing suspension culture on the hanging-drop cultured cell, thus realizing totipotency restoration of the mesenchymal stem cell. The experiment of the invention proves that, according to the method for restoring totipotency of a mesenchymal stem cell (specifically 3D culture), after the mesenchymal stem cell (in-vitro mesenchymal stem cell with reduced totipotency) subcultured multiple times is subjected to 3D culture treatment, the increased volume is gradually restored, the cell viability is enhanced, the cloning capability and differentiation capability are enhanced, and the paracrine action is also improved, thereby activating the aged mesenchymal stem cell and restoring the characteristics of the stem cell.

The invention provides a tissue engineering meniscus scaffold and a preparation method thereof. The preparation method comprises the following steps: placing a polycaprolactone material into a nozzle of a fused deposition modeling three-dimensional printer to be heated to 120-140 DEG C, and preparing for printing at the air pressure of 600-1000 kPa; setting the printing speed of the fused deposition modeling three-dimensional printer to be 0.6-0.75 mm / s, the fiber diameter to be 300-320 [mu]m and the fiber interval to be 200-300 [mu]m and printing a tissue engineering meniscus scaffold; using cobalt-60 for irradiation and sterilization treatment. As the fused deposition modeling method is adopted to print the tissue engineering meniscus scaffold with an appropriate pore structure, an appropriate fiber diameter and appropriate biomechanical strength, the survival rate and the reproduction rate of inoculated cells and autologous in-growth cells of implanted regions are favorably increased, the fibrous cartilage differentiation rate can be increased, and newly generated meniscus tissues have favorable functions.

The invention discloses an umbilical cord mesenchymal stem cell preparation and a preparation method and application thereof. Through exploration and verification by a large quantity of experiments, the 3rd-10th-generation umbilical cord mesenchymal stem cells are selected as raw materials, and by a method of dispersing tissue by a mechanical method and then performing treatment with mixed enzymes containing collagenase I, collagenase II and trypsin for a short time for many times, the vitality of the separated umbilical cord mesenchymal stem cells is guaranteed. A culture medium special for the umbilical cord mesenchymal stem cell preparation, containing DMEM / F12 (without phenol red), 10% FBS, 20 kinds of amino acids and 8 kinds of vitamins, is adopted, mixed liquor of repeated cell culture supernatant and cell lysate supernatant is collected, more umbilical cord mesenchymal stem cell active protein and active factors are obtained, the preparation cost is reduced, and industrial requirements for a stem cell product preparation are met. The prepared umbilical cord mesenchymal stem cell preparation can promote proliferation of epidermic cells and skin fibroblast, is safe and is free from toxic and side effects, an allergy does not exist, and when the umbilical cord mesenchymal stem cell preparation is applied to feature-beautifying skin-care products, the effects are notable.

The invention provides a method for culturing umbilical cord mesenchymal stem cells in a separated mode from umbilical cord outer layer amnion tissue. The method includes the steps that umbilical cord tissue of a healthy newborn is used, washed, disinfected and mechanically cut into pieces to separate an outer layer amnion, and the outer layer amnion is cultured in a suspension mode with a serum-free culture medium after being treated with erythrocyte lysate; medium change is carried out every three to five days, after the attachment rate in a plate reaches 30-70%, trypsinization is used, cells are collected centrifugally for passage amplification, and confluence is carried out when the cell confluence rate reaches 80-90% to obtain the high-purity umbilical cord mesenchymal stem cells.

The invention discloses a method for separating and extracting human subcutaneous adipose-derived mesenchymal stem cells and a special culture medium for extraction. The method comprises the following steps of: cutting up the human subcutaneous adipose tissue, centrifuging at 4 DEG C to remove big adipose tissue, and adding mixed I type and II type collagenase for water-bath digestion; suspending with the special culture medium for extraction, and performing water bath at 37 DEG C; filtering the collected cells, adding the special culture medium for extraction, and culturing primary cells for 5-7 days; culturing the cells after primary culture with a common culture medium, and optionally performing continuous culture; and adding certain concentration of fetal bovine serum, recombinant fibroblast growth factor, epidermal growth factor, insulin, acetylcysteine and thyronine, wherein the special culture medium for extraction includes a low-sugar dulbecco's modified eagle's medium. Through the invention, a great quantity of adipose-derived mesenchymal stem cells can be extracted from subcutaneous adipose, and the intracellular stem cells subjected to primary culture have higher purity and stronger activity.

The invention relates to a method for obtaining a haploid plant by chilli anther culturing, which belongs to the technical field of agriculture. The method comprises the steps of sterilizing selected flower bud, grafting anther, pretreating, culturing anther, and inducing callus tissue to be subjected to redifferentiation to form the haploid plant. According to the method, a microspore and an anther wall are co-cultured by virtue of a solid culturing medium, low temperature and heat shock pretreatment is performed, and callus induction, culturing medium component differentiation and culturing conditions are adjusted, so that the callus rate and the differentiating capability of the callus tissue are enhanced. When the method is used for culturing chilli anther, the callus inductivity reaches over 60%, the emergence rate reaches over 80% and the problem of low inductivity in chilli anther culturing is solved. By virtue of the method, the haploid regenerated plant can be obtained rapidly and the emergence rate is high; a powerful approach is provided for new variety breeding.

The invention provides a method for rapidly separating and extracting hUC-MSC (human Umbilical Cord mesenchymal stem cells). The method comprises the following steps: taking the freshly collected umbilical cord tissue of a healthy newborn baby, carrying out on-ice transportation on the freshly collected umbilical cord tissue in umbilical cord storage transportation liquid containing double antibodies, carrying out cleaning and disinfection by adopting 75% alcohol and normal saline, removing blood vessels, carrying out blunt dissection on wharton jelly, carrying out mechanical pulverization, treating the obtained product I by adopting red blood cell lysis buffer for 3 min, digesting the obtained product II by adopting IV collagenase, screening the obtained product III by adopting a 100-200-mesh sieve, carrying out suspension culture on the obtained product IV by adopting a serum-free medium, wherein the liquid is changed every 3-5 days, taking supernatant, detecting cell pollution, after the adherent rate in a plate reaches 30-70%, carrying out trypsinization, carrying out centrifugation, collecting cells, carrying out passage amplification, carrying out merging when the cell merging rate reaches 90% or above, collecting the cells, carrying out cryopreservation on the cells, and detecting the biological characteristics of hUC-MSC.

The invention provides a stem cell injection for treating osteoarthritis cartilage defect and preparation and application thereof. The injection comprises fifth-generation umbilical cord mesenchymal stem cells and a liquid solvent, wherein the fifth generation umbilical cord mesenchymal stem cells are suspended in the liquid solvent, and the concentration is (2.0-3.0)x10<7> cells / 4.5-5.0ml of the liquid solvent. The liquid solvent is normal saline, or autologous platelet-rich plasma, or a mixed solution of normal saline and human albumin, or a mixed solution of normal saline and autologous platelet-rich plasma. The human albumin accounts for 5-8% in volume by percentage. The normal saline accounts for 10% or below in volume by percentage. The injection is administered by intravenous injection or bone joint cavity local injection or the combination of the two, the cell injection amount of the intravenous injection is (1.0-1.5)x10<8> cells / one time, and the cell injection amount of the bone joint cavity injection is (2.0-3.0)x10<7> cells / one time / one knee. The effect of the stem cell injection for treating the integrity degree of osteoarthritis cartilage is obviously superior to that of a traditional hyaluronic acid (HA) method.

The various embodiments herein provide a method of co-culturing human endometrial stem cells and rat embryonic tooth bud cells to obtain ameloblast cells. The human endometrial stem cells are isolated from human females between the ages of 18-40 and cultured. The hEnSCs are identified using flowcytometry. The rat embryonic tooth bud cells are isolated from rat embryos and cultured. After the hEnSCs and rat embryonic tooth bud cells reach a desired level of cell growth, the hEnSCs are transferred to culture plate inserts and the rat embryonic tooth bud cells are transferred to a culture plate. The hEnSCs and rat embryonic tooth bud cells are co-cultured to obtain ameloblast cells. Immunocytochemical analysis is used to characterize the differentiated ameloblast cells. Quantitative real time PCR (qRT-PCR) is used to detect the presence of Ameloblastin, Amelogenin, Amelotin and Cytokeratin 14 proteins in differentiated ameloblast cells.

The invention discloses a Cyclocarya paliurus cutting seedling growing method comprising the following steps: 1, cuttingwood selecting; 2, cuttingwood processing; 3, cuttingwood planting; 4, post-planting management; the method uses specially prepared matrix, and the matrix has excellent physicochemical performance so as to provide good environment conditions for cuttingwood growth; nitration soup immersion and electric field alternative repeatedly treatment are carried out for the cuttingwood root, thus enhancing root cell splitting and differentiation capability, and improving growth vitality and anti adversity performance of the cuttingwood; combining with matrix effect and post-planting management, the novel method can largely improve cuttingwood survival rate, and the seedling is tidy and prosperous in growth vigor, thus reducing incubation time for 7-10 days when compared with same specification cutting seedlings; the method is suitable for large-area popularization plantation, and very strong in market competitiveness.

Enhanced postnatal adherent cells and a preparation method therefor are provided. The preparation method of enhanced postnatal adherent cells can increase the yield and the proliferation rate of adherent cells from placental tissues; and prepare adherent cells, which secrete proteins effective for neurological diseases and have an improved ability for movement to damaged tissues.

The invention discloses a method for increasing the grafting survival rate of a peach tree. The method is characterized by comprising the following steps of 1, branch selection, wherein the 4-5-years-old peach tree is adopted as a parent tree, and the lower portions of soft branches are bilaterally cut by 30 degrees; 2, cion treatment, wherein a cion branch is soaked in a sterilization solution and a growth promoting solution, and then soaked in plant extract; 3, stock treatment, wherein a stock is firstly treated through carbendazim solution and growth promoting solution spraying, and the wound portion of the stock is sealed with plant glue liquid; 4, grafting, wherein the stock with a wrapping layer removed and the cion are combined, a growth promoting solution and plant extract are sprayed to the combination portion, then plant glue liquid is used for repeating coating, and the combination portion is wrapped by gauze and a preservative film; 5, daily management, wherein during woundhealing, the peach tree is covered with a plastic film and a shading net, and a growth promoting solution and plant extract are used for watering and spraying.

The invention discloses an inhibition method for induced differentiation of hair follicle stem cells into vascular endothelial cells, comprising: (1), isolating rat hair follicle stem cell; (2), culturing the rat hair follicle stem cells; (3), purifying the rat hair follicle stem cells; (4), inhibiting the induced differentiation of the rat hair follicle stem cells into vascular endothelial cells. In the inhibition method provided herein, vascular endothelial cell growth factor 165 is used for the first time as an inducing factor so that the rat hair follicle stem cells efficiently and directionally differentiate into vascular endothelial cells; the formation and growth of vascular endothelial cells generated by induced differentiation of the hair follicle stem cells can be adjusted, an effective healing of a wound is promoted; it is also possible to provide seed cell sources for solving the problems in vascularization of tissue engineering skin, cell-transplant therapy of ischemic disease and the like.

The invention discloses a transgenic mesenchymal stem cell which can treat radiation enteritis and a preparation method. The CXCR4 protein can be expressed highly on the surface of transgenic mesenchymal stem cell; under the chemotaxis of stromal cell derived factor which is released from the injured intestine, the homing and colonization of a large amount of transgenic mesenchymal stem cells can promote proliferation and repair of the intestinal mucosa and inhibit apoptosis; the radiation enteritis is cured. The steps of the preparation method of the transgenic mesenchymal stem cell are that : (1)the separation and cultivation of mesenchymal stem cell; (2) the preparation of CXCR4 recombinant adenovirus: full length cDNA of CXCR4 is amplified by RT-PCR; the construction of CXCR4 recombinant shuttle plasmid and skeleton plasmid; the CXCR4 recombinant adenovirus is packaged, amplified, purified and determined by titer in HEK293T cell; (3) the transfect ion of mesenchymal stem cell by using CXCR4 recombinant adenovirus.

The invention relates to a somatic embryogenesis and plant regeneration method for Picea asperata Mast. The method comprises the following steps: collecting freely-pollinated clonal cones of the Picea asperata Mast, and isolating immature zygotic embryos for somatic embryogenesis. The somatic embryogenesis comprises four phases: an embryonic callus induction phase, an embryonic callus proliferation phase, a somatic embryo mature phase and a somatic embryo germination phase. Compared with the conventional international research on the same or similar tree species, the frequency of embryonic callus induction is greatly increased, and the differentiation capacity and germination capacity of somatic embryos are also greatly increased. Meanwhile, a long-time keeping and proliferation method for embryonic callus is obtained, thus greatly reducing the risk of embryonic loss.

The process of separating endothelial ancestral cell from human fat tissue relates to method of obtaining seed cell in tissue engineering. Morphological change and cellular immunofluorescene show that the induced cell possesses the phenotype of mature endothelial cell and function of ingesting low density lipoprotein. The detection result with flow cytometer shows that the inducing system possesses very high inducing efficiency. The induced cell forms dendrite bifurcation structure during 3D culture.

The invention discloses a preparation method and application of a silk bracket, and a three-phase silk ligament graft and a preparation method thereof. Silks are woven into a net-shaped bracket with a macroporous structure; the bracket is divided into three areas: a ligament area, a cartilage area and a bone area; mesenchymal stem cells, mesenchymal stem cells carrying with TGF-beta gene lentiviral transfection and mesenchymal stem cells carrying with BMP-2 gene lentiviral transfection are respectively planted in the three areas; the net-shaped bracket modified by the divided areas is rolled to form a bionic ligament graft with a physiological transition structure. The method avoids the problem of stress concentration due to direct connection of the soft and hard tissues; the planting gene-modified mesenchymal stem cells have excellent directional differentiation features, so that the cultivating time and steps of planting a plurality of different cells are saved, and the problem of weak biological fixation of the ligament graft-bone combination part in the existing single-phase tissue engineering ligament and the problem of the traditional method incapable of enabling the cells to grow deeply are solved.

The invention relates to the field of biotechnology and particularly relates to a menstrual blood preserving fluid and an application thereof and a separation culture method of menstrual blood-source endometrium stem cells, wherein the menstrual blood is the source of the menstrual blood-source endometrium stem cells. The menstrual blood of women is used as the source for stem cell collection, so that the waste is utilized and turned into wealth while the source is abundant; the number of endometrium stem cells in the menstrual blood is 30 times that in bone marrow, and the active ingredients are plentiful; and the menstrual blood is preserved by the menstrual blood preserving fluid so as to prevent the menstrual blood from being contaminated by the outside in a process from collection to separation. In the invention, the menstrual blood is preserved by the menstrual blood preserving fluid with a unique formula, and the contamination rate of the menstrual blood is remarkably reduced while the purity and activity of the obtained menstrual blood-source endometrium stem cells are guaranteed.

The invention relates to a method for inducing in vitro directional differentiation of stem cells by cell co-culture. An annular gasket is arranged on a bottom of a culture dish. Firstly, stem cells are inoculated in an annular zone and are cultured in an incubator. When the stem cells attach a culture dish wall, a mixed solution containing agarose and sodium alginate is added to the annular zone. When the mixed solution is solidified, a calcium chloride solution is added for a carrying out a calcification reaction and a chitosan is added for carrying out a reaction to form an agarose / sodium alginate / chitosan flat composite gel layer on the stem cells. Inductive cells are inoculated on a surface of the composite gel. By means of the co-culture of the inductive cells and the stem cells, interactions among in vivo cells and between cells and soluble factors can be simulated. By means of the polysaccharide-based agarose / sodium alginate / chitosan flat composite gel, an in vivo extracellular matrix can be simulated. The directional differentiation of the stem cells can be adjusted and controlled through rigidity and thickness of the composite gel. Meanwhile, isolated co-culture of the stem cells and the inductive cells can be achieved so that differentiated cells are easy to collect. The method has an important effect in application of regenerative medicine.

The invention discloses a fast and efficient biological technical method for separating and amplifying umbilical cord mesenchymal stem cells. According to the method, umbilical cord tissues from full-term fetus born by cesarean section are utilized to obtain a large amount of mesenchymal stem cells through a mixed enzyme digestion method combined with an explant method, in a short period of time. This method effectively shortens enzyme digestion time, reduces loss of cell number to a largest degree, and improves the influence on biological activity of mesenchymal stem cells in a long enzyme digestion process. The method also optimizes in vitro amplification conditions; and the amplified cells have the biological characteristics of mesenchymal stem cells and high proliferation ability. The invention creates a fast and efficient biological technology for separation, purification and amplification of human umbilical cord mesenchymal stem cells, and provides an adequate seed cell source for research and clinical application of stem cells.

The invention provides a method for fast separating and extracting human Umbilical Cord mesenchymal stem cells (hUC-MSC) from umbilical cord outer layer amnion tissue. The method includes the following steps that fresh collected umbilical cord tissue of a healthy newborn is taken, transported on ice in double-antibody-containing umbilical cord storing transporting liquid, washed and disinfected with 75% ethyl alcohol and normal saline, outer layer amnions are separated bluntly after blood vessels are rejected, mechanically smashed and treated with erythrocyte lysate for 3 min, IV type collagenase digestion is used, and a digestion product is cultured in a suspension mode with a serum-free culture medium after being screened through a 100-200-mesh sieve; medium change is carried out every three to five days, supernatant is taken to detect cell contamination, after the attachment rate in a plate reaches 30-70% and trypsinization is used, cell passage amplification is collected centrifugally, and confluence is carried out when the cell confluence rate reaches 90% or above, the cells are collected for cryopreservation, and the biological characteristics of hUC-MSC are detected.