14results about How to "Strong differentiation ability" patented technology

The invention discloses a pig chimeric embryo culture medium and application thereof in effectively improving the in-vitro chimeric ability of pig embryonic stem cells, and belongs to the technical field of bioengineering. According to the pig chimeric embryo culture medium disclosed by the invention, a neutral culture environment for promoting synchronous development of stem cells and embryos is created by accurately regulating and controlling growth factors, nutrient substances and other key components in the culture medium. On the basis, overexpression of anti-apoptosis factors, optimization of microinjection conditions and adoption of a laser-assisted incubation means are combined, so that the chimeric efficiency is further improved, integration of the stem cells and embryos is effectively supported, the chimeric efficiency is improved, and a reliable in-vitro tool is provided for evaluating the chimeric ability and pluripotency of the pig stem cells.

The invention belongs to the technical field of cell culture, and particularly relates to a method for promoting proliferation and differentiation of cervical squamous epithelial cells. The method comprises the following steps: S1, extracting and culturing primary cervix uteri squamous epithelial cells from cervix uteri tissues to obtain primary cervix uteri squamous epithelial cells; s2, adding the primary cervical squamous epithelial cells into a cell proliferation induction culture solution to induce cell proliferation, so as to obtain proliferated cervical squamous epithelial cells; and S3, uniformly mixing the proliferated cervical squamous epithelial cells with differentiation matrigel, and inducing cell differentiation by using a cell differentiation induction culture solution to obtain differentiated cervical squamous epithelial cells. Proliferation and differentiation of the cervical squamous epithelial cells are promoted by preparing a proper culture medium, and influence factors of proliferation and differentiation of the cervical squamous epithelial cells are researched by culturing the cervical squamous epithelial cells in vitro, so that the pathogenesis of cervical erosion and cervical cancer can be clarified; therefore, a direction is provided for research and development of medicines for treating cervical erosion and cervical cancer.

The invention discloses a yak intramuscular precursor fat immortalized cell line and a preparation method thereof. The method comprises the following steps: (1) obtaining yak primary intramuscular precursor fat cells; (2) performing SV40T lentivirus infection on the precursor fat cells in the yak muscle and screening cell strains: performing SV40T lentivirus infection by utilizing a 1 / 2 small-volume infection method to obtain immortalized cells after the precursor fat cells in the yak primary muscle are infected with SV40T, namely immortalized cells for short; puromycin is adopted to screen immortalized cells; and diluting the screened cells by using a limited dilution method, and carrying out enlarged culture to obtain the immortalized cell line. The yak intramuscular precursor fat immortalized cell line constructed by the invention has genetic homogeneity, the heterogeneity influence of cell populations is eliminated, and an in-vitro cell model is provided for subsequent research related to yak intramuscular fat cells.

This invention provides novel stem cell activators, topical skin agents containing the aforementioned stem cell activators, and skin cosmetics containing the aforementioned stem cell activators. The stem cell activator of this invention comprises at least one compound selected from compounds (including optically active agents) shown in formula (1), salts of the aforementioned compounds, and hydrates thereof as active ingredients. In the following formula, R... 1 R 2 Represents a hydrogen atom or a hydrocarbon group. R 3 Represents a hydrogen atom or an organic group. n represents an integer greater than or equal to 1. The above hydrocarbon groups may have substituents. [Chemical Formula 1]

This invention provides a method for promoting the differentiation of induced pluripotent stem cells (iPSCs) into three germ layers and its application in differentiation potential detection, relating to the field of biotechnology. The method includes seeding iPSCs into a culture medium containing 5 μM~20 μM E-cadherin activator 1, shaking culture to form embryoids, adding differentiation medium for differentiation culture, obtaining embryoids with activated three germ layer differentiation potential; seeding the embryoids with activated three germ layer differentiation potential into a container coated with a cell adhesion matrix, adding differentiation medium for further differentiation culture, inducing differentiation into three germ layer cells. This invention solves the technical problems in existing technologies where induction of iPSC differentiation into three germ layers via the embryoid pathway results in poor embryoid formation, poor embryoid state, poor long-term maintenance, and poor adhesion after embryoid transfer, leading to poor endoderm differentiation and migration abilities.

The present invention relates to the field of biotechnology, and in particular to a method for differentiating frozen umbilical cord mesenchymal stem cells into neural stem cells, comprising the following steps: 1) obtaining and preparing umbilical cord mesenchymal stem cells; 2) freezing the umbilical cord mesenchymal stem cells; 3) resuscitating and sampling the umbilical cord mesenchymal stem cells: after resuscitating the umbilical cord mesenchymal stem cells using a freezing and resuscitation box, taking out a non-destructive sampling cryopreservation tube, and detecting cell activity; 4) culturing the umbilical cord mesenchymal stem cells using an MSC culture medium until the culture dish is covered; 5) taking F3-F5 generations of umbilical cord mesenchymal stem cells in a logarithmic growth phase, digesting them, centrifuging them, and washing them to obtain mesenchymal stem cells; 3) inducing the mesenchymal stem cells obtained in step 2) into differentiated stem cells using an induction culture medium; 4) culturing the differentiated stem cells in a first differentiation culture medium at 37° C. and 5% CO2 for 3-7 days, and then transferring them to a second differentiation culture medium at 37° C. and 5% CO2 for further culturing for 3-7 days to finally obtain neural stem cells.

The present invention provides a preparation method and application of umbilical cord mesenchymal stem cells, belonging to the field of biomedical technology. By using the conditioned medium of umbilical cord blood venous endothelial cells secreting exosomes for 48 hours as the basic component, compounding the serum "containing compound Chinese medicine ingredients" as the culture system of umbilical cord mesenchymal stem cells, and compounding hypoxia culture, the obtained mesenchymal stem cells are used to treat post-stroke cognitive impairment (PSCI). The umbilical cord mesenchymal stem cells of the present invention can penetrate the brain-blood barrier and improve the ischemia and hypoxia of tissues in the cerebral infarction area, and also provide a new way of synergistic treatment of PSCI biological treatment and traditional Chinese medicine treatment.

The invention relates to an in-vitro culture medium, an in-vitro culture method and application of a human amniotic fluid source organoid. The in-vitro culture medium of the human amniotic fluid source organoid comprises a DMEM-F12 basal culture medium, a glial cell source neurotrophic factor, 8-bromoadenosine-3 ', 5'-cyclic monophosphate and aldosterone. The culture medium provided by the invention is clear in component and high in culture success rate. The human amniotic fluid organ cultured by using the culture medium and the method disclosed by the invention can be differentiated into multiple types of functional cells including neuron-like cells, myocardial cells and the like through in-vitro induction, and the differentiation capacity is obviously superior to that of an existing 2D cell line model.

This invention provides a method for preparing stem cells with enhanced osteogenic differentiation capacity, belonging to the field of biological technology. This invention significantly improves the osteogenic differentiation capacity of bone marrow mesenchymal stem cells (BMSCs) by combining deer antler polypeptide with rhodioloside. The osteogenic induction culture medium prepared in this invention includes DMEM / F12 medium, 10 mM β-glycerophosphate sodium, 50 μg / ml ascorbic acid, 100 nM dexamethasone, 20 μg / ml deer antler polypeptide, and 20 μg / ml rhodioloside. This optimized culture medium achieves a significant osteogenic promoting effect at lower concentrations, demonstrating the synergistic effect of deer antler polypeptide and rhodioloside. Simultaneously, the synergistic effect reduces the amount of components used, lowers the cost of using deer antler polypeptide, and reduces the side effects associated with high doses.

The invention discloses an in-vitro amplification kit for human peripheral blood regulatory T cells as well as a use method and application of the in-vitro amplification kit, and belongs to the technical field of cell separation and amplification. In order to solve the technical problems of low efficiency and low purity of an in-vitro amplification method of regulatory T cells in the prior art, the invention provides an in-vitro amplification kit of human peripheral blood regulatory T cells, the kit comprises a culture medium, an activation antibody, an induction factor and an amplification factor; the activated antibody is a CD3 monoclonal antibody and a CD28 monoclonal antibody, the induction factor is a recombinant human TGF-beta monoclonal antibody, an all-transretinoic acid monoclonal antibody and an anti-human IL-6 monoclonal antibody, and the amplification factor is a recombinant human IL-2. The kit provided by the invention comprises the anti-human IL-6 monoclonal antibody, so that CD4 + cells are prevented from differentiating towards Th17, and the differentiation efficiency and purity of regulatory T cells are improved; the kit has the advantages of simple components, low cost, high amplification purity and quantity, simplicity and convenience in operation and high universality, and can meet the dosage of clinical treatment experiments.

The invention provides IncRNA and application thereof in improving functions of postmenopausal mesenchymal stem cells, and belongs to the technical field of biological medicine. The lncRNA ZMYM4-AS1 provided by the invention is proved to be capable of remarkably improving the anti-oxidative stress capability, the multiplication capability, the migration capability and the differentiation capability of the mesenchymal stem cells in the body of the postmenopausal female. The improved cell functions play a crucial role in maintaining and repairing tissue damage, so that a new thought and a new method are provided for treating related diseases. The discovery not only has an important scientific value in the field of basic research, but also has a wide application prospect in clinical application, and is expected to bring a new treatment means to the health of postmenopausal women.

The present invention relates to stem cells derived from villi adjacent to chorionic plate and cell therapeutic agents containing the same. The villi adjacent to chorionic plate (VCP) tissue-derived stem cells of the present invention are characterized in that the villi adjacent to chorionic plate tissue is located in the region from the chorionic plate adjacent part of the whole villus to 1 / 3 of the distance from the chorionic plate adjacent part to the end of the villus, including the villus base part. Compared with other placental tissue-derived stem cells, they exhibit uniform growth characteristics, excellent proliferation characteristics, and significantly superior differentiation ability to cartilage, bone, and fat. Therefore, they can be effectively used in various tissue regeneration treatments required for cartilage, bone, and fat regeneration, and in particular, can be effectively used in the treatment of cartilage regeneration and osteoarthritis.

The present invention relates to the field of genetic engineering technology, and discloses an epigenetic gene editing system based on CRISPR / dCas9 and its application. The epigenetic gene editing system comprises: a superCRISPRoff fusion protein and an sgRNA that guides the superCRISPRoff fusion protein to target a target gene; the amino acid sequence of the superCRISPRoff fusion protein is shown in any one of SEQ ID NO.1~6. Compared with the existing tools dCas9‑DNMT3A‑3L or CRISPRoff, the epigenetic gene editing system of the present invention has the characteristics of high efficiency and lasting effect. In addition, the epigenetic gene editing system of the present invention can significantly inhibit the expression of myostatin in muscle satellite cells, improve the differentiation ability of muscle satellite cells, and thus promote muscle growth.

The invention discloses application of fructo-oligosaccharide in improvement of growth performance and intestinal development of suckling piglets and a nutrition regulator of the fructo-oligosaccharide, the fructo-oligosaccharide is applied to the suckling piglets as an early nutrition intervention means, the intestinal villus height of the suckling piglets is remarkably increased, the crypt depth is reduced, and the growth performance of the suckling piglets is improved. The improvement of the activity of small intestine disacidase and the change of small intestine stem cell differentiation marker gene mRNA indicates that the fructo-oligosaccharide improves the growth performance of the suckling piglets by promoting the intestinal tissue morphology, digestion and absorption functions and small intestine stem cell differentiation capacity of the suckling piglets.