4results about How to "Strong differentiation ability" patented technology

This invention provides novel stem cell activators, topical skin agents containing the aforementioned stem cell activators, and skin cosmetics containing the aforementioned stem cell activators. The stem cell activator of this invention comprises at least one compound selected from compounds (including optically active agents) shown in formula (1), salts of the aforementioned compounds, and hydrates thereof as active ingredients. In the following formula, R... 1 R 2 Represents a hydrogen atom or a hydrocarbon group. R 3 Represents a hydrogen atom or an organic group. n represents an integer greater than or equal to 1. The above hydrocarbon groups may have substituents. [Chemical Formula 1]

This invention provides a method for promoting the differentiation of induced pluripotent stem cells (iPSCs) into three germ layers and its application in differentiation potential detection, relating to the field of biotechnology. The method includes seeding iPSCs into a culture medium containing 5 μM~20 μM E-cadherin activator 1, shaking culture to form embryoids, adding differentiation medium for differentiation culture, obtaining embryoids with activated three germ layer differentiation potential; seeding the embryoids with activated three germ layer differentiation potential into a container coated with a cell adhesion matrix, adding differentiation medium for further differentiation culture, inducing differentiation into three germ layer cells. This invention solves the technical problems in existing technologies where induction of iPSC differentiation into three germ layers via the embryoid pathway results in poor embryoid formation, poor embryoid state, poor long-term maintenance, and poor adhesion after embryoid transfer, leading to poor endoderm differentiation and migration abilities.

This invention provides a method for preparing stem cells with enhanced osteogenic differentiation capacity, belonging to the field of biological technology. This invention significantly improves the osteogenic differentiation capacity of bone marrow mesenchymal stem cells (BMSCs) by combining deer antler polypeptide with rhodioloside. The osteogenic induction culture medium prepared in this invention includes DMEM / F12 medium, 10 mM β-glycerophosphate sodium, 50 μg / ml ascorbic acid, 100 nM dexamethasone, 20 μg / ml deer antler polypeptide, and 20 μg / ml rhodioloside. This optimized culture medium achieves a significant osteogenic promoting effect at lower concentrations, demonstrating the synergistic effect of deer antler polypeptide and rhodioloside. Simultaneously, the synergistic effect reduces the amount of components used, lowers the cost of using deer antler polypeptide, and reduces the side effects associated with high doses.

The invention discloses application of fructo-oligosaccharide in improvement of growth performance and intestinal development of suckling piglets and a nutrition regulator of the fructo-oligosaccharide, the fructo-oligosaccharide is applied to the suckling piglets as an early nutrition intervention means, the intestinal villus height of the suckling piglets is remarkably increased, the crypt depth is reduced, and the growth performance of the suckling piglets is improved. The improvement of the activity of small intestine disacidase and the change of small intestine stem cell differentiation marker gene mRNA indicates that the fructo-oligosaccharide improves the growth performance of the suckling piglets by promoting the intestinal tissue morphology, digestion and absorption functions and small intestine stem cell differentiation capacity of the suckling piglets.