Stem cell injection for treating osteoarthritis cartilage defect and preparation and application thereof

A stem cell technology for osteoarthritis, applied in the field of stem cell injection and its preparation for the treatment of cartilage defects in osteoarthritis, to achieve the effect of large number of cells, promotion of damage repair, and good microbiological and biological safety

Pending Publication Date: 2019-09-17
WUHAN HAMILTON BIOTECH
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

However, due to the limited survival time of chondrocytes, limited donor sources, immunogenicity of allografts, and joint fibrosis complications, it is urgent to study new treatment methods for knee osteoarthritis and improve its clinical treatment effect.

Method used

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  • Stem cell injection for treating osteoarthritis cartilage defect and preparation and application thereof
  • Stem cell injection for treating osteoarthritis cartilage defect and preparation and application thereof
  • Stem cell injection for treating osteoarthritis cartilage defect and preparation and application thereof

Examples

Experimental program
Comparison scheme
Effect test

Embodiment 1

[0079] Example 1: The above-mentioned cultured human umbilical cord mesenchymal stem cells are used to prepare stem cell injection for treating arthritis. The specific preparation process is as follows:

[0080] Dispense the washing solution (DPBS) into 15ml centrifuge tubes for later use; turn on the centrifuge and adjust to 1900rpm for 6min; prepare the liquid medium: autologous platelet-rich plasma, human albumin, cell strainer, normal saline; Take out the corresponding number of cryopreserved cells from the nitrogen tank, and quickly transfer them to a 37°C water bath; when the ice cubes in the cell cryopreservation tubes are as large as soybeans (water bath time 1.5-2.5 minutes), take out the cryopreservation tubes, and quickly transfer them to the preparation Area. Wipe the cryovial with sterile alcohol for disinfection, and unscrew the cap; transfer the cells to the prepared washing solution with a pipette gun, centrifuge at 1900rpm for 6min. After centrifugation, remo...

Embodiment 2

[0086] Example 2: Study on the curative effect of P5 generation UC-MSCs on knee osteoarthritis animal model In this experiment, the cell therapy composition 1 (stem cells + normal saline) prepared in Example 1 was selected to investigate its effect on knee osteoarthritis Curative effect, its specific experimental process is as follows:

[0087] Select 60 adult healthy male Wistar rats (weight 175g~200g) and divide them into four groups according to random number table method: normal group (n=15), model group (n=15), stem cell treatment group (n=15), HA treatment group (n=15). The normal group was rats without any treatment, the model group was rats with knee osteoarthritis induced by sodium iodoacetate (Monosodium iodoacetate, MIA), the stem cell treatment group was rats injected with UC-MSCs into the knee joint cavity, and the rats treated with HA In the group, HA was injected into the knee joint cavity. After modeling, 14 days after knee osteoarthritis. The stem cell ther...

Embodiment 3

[0090] Example 3: Clinical application of the composition 3 (stem cells + autologous platelet-rich plasma) prepared in Example 1 to knee osteoarthritis Case 1:

[0091] Basic patient information: Patient Zhao XX, male, 51 years old, occupation: policeman / martial arts athlete. Physical examination in November 2018, chief complaint: repeated knee joint pain for 20+ years, aggravated for 4 years; current medical history: patient's double knee joint pain was gradually aggravated due to long-term heavy exercise, and both knee joint pains were felt when walking and resting , especially on the right. The movement of the right knee joint was accompanied by popping sound. Acupuncture, local injection of sodium hyaluronate and other treatments had no obvious effect. The pain gradually increased in the past 4 years, accompanied by deformity of the right knee joint. Since the onset, the spirit, food and drink are normal, and the sleep is not good enough. Past history: In 2014, the right...

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Abstract

The invention provides a stem cell injection for treating osteoarthritis cartilage defect and preparation and application thereof. The injection comprises fifth-generation umbilical cord mesenchymal stem cells and a liquid solvent, wherein the fifth generation umbilical cord mesenchymal stem cells are suspended in the liquid solvent, and the concentration is (2.0-3.0)x10<7> cells/ 4.5-5.0ml of the liquid solvent. The liquid solvent is normal saline, or autologous platelet-rich plasma, or a mixed solution of normal saline and human albumin, or a mixed solution of normal saline and autologous platelet-rich plasma. The human albumin accounts for 5-8% in volume by percentage. The normal saline accounts for 10% or below in volume by percentage. The injection is administered by intravenous injection or bone joint cavity local injection or the combination of the two, the cell injection amount of the intravenous injection is (1.0-1.5)x10<8> cells/ one time, and the cell injection amount of the bone joint cavity injection is (2.0-3.0)x10<7> cells/ one time/ one knee. The effect of the stem cell injection for treating the integrity degree of osteoarthritis cartilage is obviously superior to that of a traditional hyaluronic acid (HA) method.

Description

technical field [0001] The invention belongs to the field of biomedicine and biotechnology, and in particular relates to a stem cell injection for treating osteoarthritis cartilage defect and its preparation method and application. Background technique [0002] Knee osteoarthritis (KOA) is the most common clinical chronic arthritic disease, a degenerative disease whose incidence increases significantly with age. The pathological manifestations of KOA are the reduction of subchondral nutrient vessels, abnormal synthesis and decomposition of cartilage matrix proteins, and eventually lead to the degeneration and apoptosis of articular chondrocytes, articular cartilage defects, and inflammation infiltration of cartilage tissue; clinical manifestations are slowly developing joint pain, swelling, Stiffness, limited mobility, and joint deformity. With the prolongation of human life expectancy and the gradual aging of the population, KOA has become a serious public health problem, ...

Claims

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Application Information

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Patent Type & Authority Applications(China)
IPC IPC(8): A61K35/28A61K9/10A61P19/02A61P29/00C12N5/0775
CPCA61K35/28A61K9/10A61K9/0019A61P19/02A61P29/00C12N5/0665
Inventor 饶巍张权肖翠红施煜周端鹏石亮王尉武栋成
Owner WUHAN HAMILTON BIOTECH
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