3results about How to "Vigorous" patented technology

This invention provides a method for mutagenesis of *Aspergillus cristatus* and its application, belonging to the field of microbial technology. The mutagenesis method provided by this invention includes the following steps: first, pretreating *Aspergillus cristatus* suspension by mixing it with a compound enzyme solution; then culturing the pretreated *Aspergillus cristatus* in a culture medium supplemented with a mutagen; the compound enzyme solution consists of a cell-lysing enzyme solution and a β-glucanase solution; the mutagen includes ethyl methanesulfonate and / or diethyl sulfate. This invention can improve the efficiency of the mutagen entering the bacterial cells, thereby enhancing the mutagenesis effect; the mutant strains obtained by mutagenesis, compared to the original strain, exhibit more vigorous colony growth on plates, larger average diameter, denser hyphae, and / or better morphological stability. Furthermore, it can achieve ideal mutagenic selection pressure at lower mutagen concentrations and shorter treatment times, reducing sublethal damage, which is beneficial for the recovery and screening of mutant strains and improving the efficiency of mutagenesis experiments.

The present application relates to the field of aquaculture breeding technology, and discloses an indoor cement pool artificial breeding method for east wind snail, which comprises the following steps: storing east wind snail egg sacs after disinfecting water body by ultraviolet rays, removing infected egg sacs, and performing specific water flow replacement 24 hours before hatching; feeding the larvae with a nutrient agent containing clostridium aceticum protein and agaricus blazei polysaccharide every day after hatching, feeding the larvae with monosomic algae such as chrysomonad, tetraselmis, chlorella, etc. in the early stage, and feeding the larvae with shelled algae in the middle stage until the larvae are attached and metamorphosed; keeping the breeding pool continuously aerated and controlling parameters such as dissolved oxygen, salinity, temperature and pH value of the water body during the breeding period, and finally collecting the snail seedlings to end the breeding. The indoor cement pool artificial breeding method for east wind snail provided by the present application is suitable for one or more of fasciolaria fasciolaris and mud east wind snail, and can effectively improve the success rate and seedling quality of indoor cement pool breeding of east wind snail.

The present application relates to a kind of special strain for solid liquefaction is used to cultivate Ganoderma lucidum of linden and the method for obtaining spore powder, specifically: the present application discloses a kind of Ganoderma lucidum linden cultivation special strain for solid liquefaction medium and preparation method thereof, the medium is composed of the following weight parts of component: wheat starch 15~20 parts, glucose 1~2 parts, wheat cellulose 45~60 parts, bran 15~25 parts, yeast extract 1~2 parts, beef peptone 1~2 parts, potassium dihydrogen phosphate 1~2 parts, 100 parts of water.The present application also provides the special strain cultivation method for solid liquefaction of Ganoderma lucidum using the above-mentioned medium.The method of the present application can improve the breeding efficiency;The obtained mycelium is strong, and the species can be controlled, reduces the risk of variation.