6results about How to "Vigorous" patented technology

The invention belongs to the technical field of noodles, and discloses probiotic golden corn noodles and a preparation method thereof, and the method comprises the following steps: preparing probiotic corn fermented flour and preparing the probiotic golden corn noodles. The preparation of the probiotic corn fermented flour comprises the steps of strain activation and amplification culture, preparation of a mixed leavening agent, preparation of raw materials, inoculated fermentation, cleaning, pulping, curing and mixing, drying and grinding. The preparation method of the probiotic golden corn noodles comprises the steps of raw material preparation, dough preparation and noodle forming. The probiotic golden corn noodles disclosed by the invention have the beneficial effects of promoting intestinal health, controlling blood sugar and blood fat, enhancing immunity and improving mouth feel and flavor, so that the probiotic golden corn noodles become a healthy and delicious food choice.

This invention provides a method for mutagenesis of *Aspergillus cristatus* and its application, belonging to the field of microbial technology. The mutagenesis method provided by this invention includes the following steps: first, pretreating *Aspergillus cristatus* suspension by mixing it with a compound enzyme solution; then culturing the pretreated *Aspergillus cristatus* in a culture medium supplemented with a mutagen; the compound enzyme solution consists of a cell-lysing enzyme solution and a β-glucanase solution; the mutagen includes ethyl methanesulfonate and / or diethyl sulfate. This invention can improve the efficiency of the mutagen entering the bacterial cells, thereby enhancing the mutagenesis effect; the mutant strains obtained by mutagenesis, compared to the original strain, exhibit more vigorous colony growth on plates, larger average diameter, denser hyphae, and / or better morphological stability. Furthermore, it can achieve ideal mutagenic selection pressure at lower mutagen concentrations and shorter treatment times, reducing sublethal damage, which is beneficial for the recovery and screening of mutant strains and improving the efficiency of mutagenesis experiments.

The present application relates to the field of aquaculture breeding technology, and discloses an indoor cement pool artificial breeding method for east wind snail, which comprises the following steps: storing east wind snail egg sacs after disinfecting water body by ultraviolet rays, removing infected egg sacs, and performing specific water flow replacement 24 hours before hatching; feeding the larvae with a nutrient agent containing clostridium aceticum protein and agaricus blazei polysaccharide every day after hatching, feeding the larvae with monosomic algae such as chrysomonad, tetraselmis, chlorella, etc. in the early stage, and feeding the larvae with shelled algae in the middle stage until the larvae are attached and metamorphosed; keeping the breeding pool continuously aerated and controlling parameters such as dissolved oxygen, salinity, temperature and pH value of the water body during the breeding period, and finally collecting the snail seedlings to end the breeding. The indoor cement pool artificial breeding method for east wind snail provided by the present application is suitable for one or more of fasciolaria fasciolaris and mud east wind snail, and can effectively improve the success rate and seedling quality of indoor cement pool breeding of east wind snail.

The present application relates to a kind of special strain for solid liquefaction is used to cultivate Ganoderma lucidum of linden and the method for obtaining spore powder, specifically: the present application discloses a kind of Ganoderma lucidum linden cultivation special strain for solid liquefaction medium and preparation method thereof, the medium is composed of the following weight parts of component: wheat starch 15~20 parts, glucose 1~2 parts, wheat cellulose 45~60 parts, bran 15~25 parts, yeast extract 1~2 parts, beef peptone 1~2 parts, potassium dihydrogen phosphate 1~2 parts, 100 parts of water.The present application also provides the special strain cultivation method for solid liquefaction of Ganoderma lucidum using the above-mentioned medium.The method of the present application can improve the breeding efficiency;The obtained mycelium is strong, and the species can be controlled, reduces the risk of variation.

The invention belongs to the technical field of fermented tea drinks, and discloses a formula and a production process of a fermented tea drink, the fermented tea drink comprises water, puree apple vinegar, brown rock sugar and fermented black tea, the fermented black tea contains three probiotics of saccharomycetes, acetic bacteria and lactic acid bacteria, and can regulate the intestinal flora balance of a human body. Lactic acid bacteria can inhibit growth of harmful bacteria, acetic acid generated by acetic acid bacteria can improve the acid environment of intestinal tracts, and breeding of beneficial bacteria is facilitated. The tea polyphenol has an anti-oxidation effect and can scavenge free radicals and protect immune cells from being damaged. The polysaccharide and the isomaltooligosacharide can activate immune cells. The theophylline can promote catabolism of fat, reduce the content of cholesterol and triglyceride in blood and prevent cardiovascular diseases such as atherosclerosis, and also contains various vitamins such as B3, B12 and the like which play an important role in metabolism, nervous system function and the like of a human body and can supplement nutritional ingredients required by the human body in daily life, so that the health-care food has a good health-care effect. And practical application and operation are facilitated.

The invention belongs to the field of microorganisms, particularly relates to a screening and cultivating method of grifola frondosa mutant strains, and aims to solve the problem that high-quality grifola frondosa germplasm resources are lacked in Heilongjiang regions. The mutant strain is named as hiB3, and the preservation number of the mutant strain is CCTCC (China Center For Type Culture Collection) NO: M 20242287. The mutagenesis mode is carbon ion beam irradiation, 80 MeV / u. The hypha rapid germination solid culture medium is PDA + peptone + beef extract + yeast powder + monopotassium phosphate, and the culture temperature is 27 DEG C. The mycelium rapid propagation liquid culture medium is glucose, corn flour, soybean flour and wheat bran, and the culture temperature is 27 DEG C. The third-level strain raw material formula comprises a self-made fermentation material, corncob, wheat bran and quick lime. The hiB3 strain is high in activity, high in growth speed, early in fruiting, high in yield, resistant to high temperature and suitable for large-area cultivation in the Heilongjiang region.