Solid fermentation method for cordyceps sinensis

A technology of Cordyceps sinensis and solid fermentation method, which is applied in botany equipment and methods, food science, food preservation, etc. It can solve the problems of large energy consumption and achieve the effects of low water content, low cost and short cultivation time

Active Publication Date: 2010-04-21
SHANGHAI ZHICAO BIOTECH
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

This patent uses a solid fermenter to replace a liquid fermenter, allowing Trichosporum sinensis to grow on solid substrates such as sucrose, yeast, and silkworm chrysalis powder. The final product is a solid culture, which is easy to store, and there is no waste liquid discharge during production, but This method still needs to use a fermentation tank, and it still needs to pressurize sterile air into the tank, which consumes a lot of energy.

Method used

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  • Solid fermentation method for cordyceps sinensis
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  • Solid fermentation method for cordyceps sinensis

Examples

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Effect test

Embodiment 1

[0036] 1. Strain preparation

[0037] 1. Strains: The strains produced are Hirsutella sinensis Liu, Guo, Yu et Zeng, purchased from the Institute of Microbiology, Chinese Academy of Sciences or the Institute of Economic Insects and Fungi, Anhui Agricultural University, and tested by DNA. The similarity rate between its RDNA nucleotide sequence and natural Cordyceps sinensis is 99%;

[0038] 2. Expansion and reproduction of strains:

[0039] (1). Agar slant: agar slant medium formula:

[0040] Mushroom sugar 20g, peptone 3g, yeast extract powder 2g, potassium dihydrogen phosphate 0.5g, magnesium sulfate 0.3g, agar 20g, water 1000ml, pH 6.5, after the agar melted, put it into 100ml small triangular flasks, and culture in each bottle Base 50ml, sterilization: 121°C, 25 minutes. Inoculate with Mortierella chinensis yy0701 strain and culture at 16°C for 70 days;

[0041] *Mushroom sugar, also known as trehalose, English name: Trehalose, the sugar is ubiquitous in insects and al...

Embodiment 2

[0056] The strain preparation and the liquid strain production method in this embodiment are all the same as in Example 1. However, the inoculation method under the solid fermentation production item adopts the seed dressing method, that is, the mixing of the liquid bacteria and the solid fermentation culture material is completed in a stirrer, and the shape of the stirrer is cylindrical or conical. 60cm in diameter, 120cm in length, with a feeding port on the top and a discharge port on the bottom, can be closed, can withstand 1.1Kg / cm2 pressure, can be sterilized by steam, single-layer or jacketed, and there is a stirring shaft in the center of the agitator, which can Stir the liquid bacteria and solid culture material evenly, and push the mixture into the fermentation bag in batches. The amount of liquid bacteria used in seed dressing is: per 100Kg of solid fermentation culture material (calculated by wet weight) mixed with liquid Strain 10±2L; the plastic bag (fermentation...

Embodiment 3

[0059] The strain preparation and solid fermentation production methods in this example are the same as those in Example 2, but the expansion of liquid strains is carried out in large-capacity serum bottles. The method and steps are as follows:

[0060] Take the Mortierella sinensis lawn (about 3 square centimeters large) that has been cultured for 90 days at 16°C, cut it into 10-20 small pieces with a scalpel, and transfer it into a 500ml conical flask filled with 200ml liquid medium. The liquid parent species of Trichosporum sinensis was obtained by shaking flask culture;

[0061] 2. Get the 400ml liquid parent species in the above two 500ml garden conical flasks, and move it into the 1500ml large garden conical flask with 1000ml liquid medium in it. The inoculum size is 28%. Then put it on a reciprocating shaker for vibration cultivation, with an amplitude of 8cm and a frequency of 1000ml. 80-100 times / min; the culture temperature is 16±2°C, and after shaking culture for 6...

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Abstract

The invention discloses a solid fermentation method for cordyceps sinensis. The method adopts fermented liquor the seed lot of which is Hirsutella sinensis Liu, Guo, Yu et Zeng as a liquid strain to enable cordyceps sinensis to be fermented and produced on a solid culture medium taking grains as the matrix by pouring or blending inoculation. The method combines the advantages of both liquid fermentation and solid fermentation. The method has the advantages of short strain cultivating time, high activity and good flowability and many development points and has the characteristics of small investment of production equipment, low energy consumption, no waste liquor discharge, easy storage of products and the like. The final product of the invention is the grain being rich in cordyceps sinensis and can be directly used as raw material of health-care food.

Description

technical field [0001] The invention relates to biotechnology, in particular to a solid fermentation production method of Cordyceps sinensis. Background technique [0002] Cordyceps sinensis (Ophiocordysepes sinensis=Cordycepes sinensis (Berk.) Sacc.) is a complex of fungi and dead bodies parasitic on bat moth larvae. The "grass" on Cordyceps sinensis is the sexual organ of the fungus - the sub-seat, and the "worm" is the dead body of its host, and the inside is the mycelium-sclerotia of the fungus. The bacterial classification obtained from Cordyceps sinensis is the anamorph of Cordyceps sinensis, and the bacterial classification scientific name is Hirsutella sinensis Liu, Guo, Yu et Zeng, commonly known as Cordyceps sinensis fungus (Mycology Acta, 1988, 8 (1), 35- 40). [0003] There have been many patent reports on the fermentation production of Cordyceps sinensis. Such as application numbers 97110448.4, 03131904.1, 200410019092.4, 200410025517.2, 200410101728.X, 20071...

Claims

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Application Information

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Patent Type & Authority Applications(China)
IPC IPC(8): A01G1/04C05G1/00A23L3/44A23L1/29A23L33/00
Inventor 杨庆尧
Owner SHANGHAI ZHICAO BIOTECH
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