207 results about "Hirsutella" patented technology

The invention provides a group of relevant enzymes for preparing mannitol by performing anabolism on Chinese caterpillar fungus serving as a multifunctional production fungus and hirsutella sinensis based on glucose, a gene for encoding these enzymes and application thereof. The relevant enzymes include (1) hexokinase: manA1-A6 proteins of which the sequences are SEQ ID No.1-6, (2) phosphoglucoisomerase: manB1-B3 proteins of which the sequences are SEQ ID No.7-9, and (3) mannitol-1-P dehydrogenase: manC protein of which the sequence is SEQ ID No.10. In the invention, detailed researches are performed on the metabolic pathway of mannitol synthesized by using Chinese caterpillar fungus serving as a multifunctional production fungus, hirsutella sinensis and glucose on the aspect of principle, cloned DNA (Deoxyribose Nucleic Acid) comprising a nucleotide sequence provided by the invention can be transferred into engineering bacteria with transduction, conversion and conjugal transfer methods, and host mannitol is endowed with high expression by regulating the expression of a biosynthetic gene of the mannitol.

The present invention discloses a fermenting production process of Hirsutella hepiali Chen & Shen for industrial purpose. It contains steps: a. Isolating new stain from original source; b. identifying whether the stain can grow stroma or not; c. culturing the strain in solid medium for rejuvenescence purpose; d. Secondly culturing the strain in liquid culture medium; e. fermenting. Said method provides a fermenting production process, which can continually identify whether the anamorphic fungi related to Chinese Cordyceps sinensis change or not, whether it retains the property of original strain or not. It also can be modified continually. According to these processes, the quality of the obtained product will be stable and the property will be retained stable for quite a long time. Therefore this method overcome the problems exist in the art that change of the strain, instability of quality of the product and so on.

The invention relates to an artificial culture method of cordyceps longissima sporocarp. A strain is cordyceps longissima agamotype-hirsutella longissima. The artificial culture method of the cordyceps longissima sporocarp includes A, cant tube strain activation culture; B, liquid shake flask seed culture; C, hypha stage culture; and D, sporocarp culture. The cordyceps longissima sporocarp cultured artificially has rodlike stroma and is solitary or branching and cinnamon, length is 1-7cm, the diameter is 1-3mm, a large amount of white coremium is grown at the top end or the side face of the stroma at later stage of sporocarp culture and is compact. The artificial culture method of the cordyceps longissima sporocarp is initiated, can perform large-scale culture of the cordyceps longissima sporocarp, solves the problem that wild cordyceps longissima sources are deficient, establishes a foundation for comprehensive development of cordyceps longissima edible value and industrialized production, provides technical support, and is simple in process, low in cost, wide in raw material source, non-toxic, free of pollution and capable of performing large-scale production.

The invention belongs to a method for cultivating cordyceps sinensis stroma, and particularly relates to a method for cultivating cordyceps sinensis stroma by using hirsutella sinensis. The method comprises the following steps: soaking maize pulp, soybean meal, wheat grains, rice grains, sorghum grains, silkworm chrysalis meal or crushed whole egg and beef peptone in water; mixing and filtering the soaked raw materials and auxin to obtain filter liquor; and inoculating the soaked materials to the hirsutella sinensis liquor, then cultivating the mixture in dark place, adding the filter liquor to the mixture, and cultivating the mixture in a greenhouse to obtain the cordyceps sinensis stroma. The method has the following advantages that: the asexual hirsutella sinensis strains are inoculated to a manually prepared culture medium so as to grow the stroma in batch, and the stroma can be cyclically grown. The biological conversion rate of the cultivated cordyceps sinensis stroma is about 40 percent, and the cordyceps sinensis stroma contains over 0.02 percent of adenosine C10H13N504 and over 0.10 percent of cordycepin C10H13N503.

A solid fermenting process of cordyceps features use of Hirsutella sinensis (CCTCC No.M87101) is used as the fermenting bacterial strain, and includes bacterial strain culture, solid fermenting culture, discharging mycelia, drying and pulverizing. Its advantages are high output rate (20-40%), no environmental pollution and low cost.

The invention discloses an original ecological separation method of cordyceps sinensis hirsutella sinensis. The original ecological separation method comprises a tissue separation method and an ascospore separation method. According to the method, seed sources are selected from fresh and ripening cordyceps sinensis in Yushu, Qinghai in China, and soil with the depth being 8 to 12 cm in a range with the diameter being 10 to 12cm around the sporocarp is remained during the collection; a culture medium is from living bodies of cordyceps sinensis host of swift moth larvae, white strong and living swift moth larvae in 4 to 5 age periods are selected, and sterile water is used for rinsing and storage and placing in time during the collection; the cordyceps sinensis and the living bodies of the cordyceps sinensis host of swift moth larvae are stored at the temperature being 0 to 4 DEG C for 0 to 15 days after the collection; the seed sources are transferred onto the culture medium for culture, and the parameters are controlled as follows during the culture: the temperature is 6 to 9 DEG C, the pH value is 6 to 6.5, the light illumination is 50 to 100lux, the air humidity is 55 to 60 percent, and the oxygen content is 20 percent.

The invention discloses a Hirsutella hepiali Chen et Shen fungus which can be fermented at normal temperature, the fungus in systematics is named as (Hirsutella hepiali Chen et Shen)BS-1, and is mutagenized by the following method: protoplast is extracted from Hirsutella hepiali Chen et Shen strain which is separated from the stroma of the natural Cordyceps sinensis(Berk)Sacc., and the protoplast is irradiated by a 15-60W ultraviolet lamp at a place which is 10-50cm from the ultraviolet lamp for 1-300s, inoculated on MYG reproduction culture medium and cultured in a thermotank at the temperature of 25-28 DEG C; the mutagenized and cultured strains are inoculated in fermentation medium and acclimatized at the temperature of 25-28 DEG C for ten generations, and the contents of the active components are determined, the strains which grow well and have high strain powder yield and high contents of the active components are selected, and finally the Hirsutella hepiali Chen et Shen fungus strain which can be fermented at normal temperature is obtained. The invention also discloses a mutagenesis breeding method of the fungus and a fermentation process. The Hirsutella hepiali Chen et Shen strain can rapidly grow at normal temperature with short fermentation period and low production cost, so that the product has greater market competitiveness.

The invention discloses health capsules containing edible mushrooms and process for preparation, wherein the raw materials include ganoderma lucidum, Hirsutella hepiali Chen et Shen, hedgehog hydnum and schisandra fruit. The capsules have good effect in improving human immunity and protecting liver from chemical damages.

The present invention provides a set of cordycepin anabolism-related enzymes from bering producing strain cordyceps sinensis hirsutella sinensis-participated ADP ribose starting, genes encoding the enzymes, and application thereof. The related enzymes include proteins shown as SEQ ID No. 1-17, and the coding genes correspond to nucleotide sequences shown as SEQ ID No. 18-34. The invention conducts detailed researches on the principle of metabolic pathways of cordycepin synthesis from bering producing strain cordyceps sinensis hirsutella sinensis ADP ribose. The cloned DNA of the nucleotide sequences provided by the invention can be transferred into engineering bacteria by a transduction, transformation and conjugal transfer method. By adjusting the expression of cordycepin biosynthesis genes, high expression of cordycepin for the host is given.

Compositions comprising Hirsutella sinensis mycelia extracts and chromatographically separated polysaccharide-enriched fractions thereof are provided. Methods for extracting Hirsutella sinensis mycelia are provided. Compositions for methods for their use in amelioration, prevention and treatment of sepsis, acute endotoxemia and inflammatory responses are disclosed.

The invention provides a method for preparing a cordyceps polysaccharide biological preparation, and relates to a preparation method of preparations. The method comprises the following steps: preparing a cordyceps taishanensis culture medium: culturing cordyceps taishanensis, coarsely extracting cordyceps polysaccharide, purifying cordyceps polysaccharide; molding, namely cutting bitter gourd, fagopyrum tararicum, radix ophiopogonis, cyclocarya paliurus, alkekengi and purslane into dices, baking at 60 DEG C, and crushing; sufficiently mixing the following raw materials in percentage by weight: 30 percent of cordyceps polysaccharide, 25 percent of alkekengi, 15 percent of cyclocarya paliurus, 10 percent of fagopyrum tararicum, 10 percent of bitter gourd, 5 percent of radix ophiopogonis and 5 percent of purslane; combining medicinal auxiliaries to prepare the preparatin. According to the cordyceps polysaccharide biological preparation, cordyceps polysaccharide extracted from artificially cultured hirsutella sinensis mycelium is adopted as a main raw material, and is compatible with alkekengi, cyclocarya paliurus, fagopyrum tararicum, bitter gourd, radix ophiopogonis and purslane, so that the blood glucose reducing effect of the biological preparation is better that that of single cordyceps polysaccharide. The cordyceps polysaccharide biological preparation can be used for comprehensively reducing blood glucose, does not have toxic or side effect, is low in price, and is suitable for large-scale production.