207 results about "Hirsutella" patented technology

The invention provides a group of relevant enzymes for preparing mannitol by performing anabolism on Chinese caterpillar fungus serving as a multifunctional production fungus and hirsutella sinensis based on glucose, a gene for encoding these enzymes and application thereof. The relevant enzymes include (1) hexokinase: manA1-A6 proteins of which the sequences are SEQ ID No.1-6, (2) phosphoglucoisomerase: manB1-B3 proteins of which the sequences are SEQ ID No.7-9, and (3) mannitol-1-P dehydrogenase: manC protein of which the sequence is SEQ ID No.10. In the invention, detailed researches are performed on the metabolic pathway of mannitol synthesized by using Chinese caterpillar fungus serving as a multifunctional production fungus, hirsutella sinensis and glucose on the aspect of principle, cloned DNA (Deoxyribose Nucleic Acid) comprising a nucleotide sequence provided by the invention can be transferred into engineering bacteria with transduction, conversion and conjugal transfer methods, and host mannitol is endowed with high expression by regulating the expression of a biosynthetic gene of the mannitol.

The invention provides a total artificial method for cultivating the cordyceps sinensis, and belongs to the field of the total artificial cultivation of the cordyceps sinensis. The method comprises the steps of: a. preparing a Chinese hirsutella suspension; b. acquiring, propagating and raising the host insect; c. infecting: taking the hepialus larva obtained by the step b, and soaking the suspension of the step a by a needle-shaped apparatus with the diameter which is less than 0.2mm at one end to prick the hepialus larva to complete the infection; and d. continuously raising. After the method is used, the improper mortality of the chrysalis can be reduced, and the eclosion rate of the hepialus is improved; the mating rate of the hepialus can be improved; and the infection rate of the Chinese hirsutell to the host hepialus larvae can be greatly improved, and the total artificial industrial production of the cordyceps sinensis can be realized.

This invention describes the large-scale fermentation and processing of Chinese caterpillar fungus. In this invention, hirsutella sinensis strains are used as the fermentation strain and cultivated in the inclined plate and shaking-bottle. 3-5% of potato, 0.5-2% of soybean powders, 0.01-0.02% of soifnum muicfun, 0.01-0.02% of Chinese yam, 0.5-2% of protein, 0.5-5% of glucose, a small amount of dipotassium hydrogen phosphorus and magnesium sulfate, and water culture medium are successively directed into all levels of fermenter for fermentation and processing. The yield of the fermented product is 20% higher than that in the present processes, the fermentation periodic time is 12 days shorter, the cost is 20% lower and the pollution rate is 2% lower. Through the DNA molecular sequence examination by the institute of microbes, Chinese Academy of Sciences, the constitution of the fermented product is the same with that of natural Chinese caterpillar fungus, and the content of cordycepin, adenosine, Chinese caterpillar fungus polyoses, sterol, biological base, amino acids and trace elements is much higher than that in natural Chinese caterpillar fungus. The pharmacological action of the fermented product is better than that of natural Chinese caterpillar fungus.

The invention provides a method of full-artificial culturing Cordyceps sinensis, including sampling, strain separating, host insect collecting, breeding and feeding, infecting and continuing feeding, on artificial condition, largely culturing Hirsutella sinensis; feeding host insects in an scale room at low altitude, completing the infection of Hirsutella sinensis to the host insects and sexuality of Cordyceps sinensis. By artificial culture, the host grubs are big and good-quality, and the success rate of infection is high, the infection time is short, the quality is stable and controllable, and the pesticide effect test fully indicates that the pesticide effect of the produced Cordyceps sinensis is equivalent to that of the natural Cordyceps sinensis and it can overcomes the disadvantages of long growing time of natural Cordyceps sinensis and low natural infection ratio, and implements the industrialized production of Cordyceps sinensis.

The invention provides a method for increasing the yield of natural cordyceps sinensis. The method comprises the following steps of (1) selecting a base for increasing the yield of natural cordyceps sinensis; (2) placing a trapping lamp; (3) preparing trapping liquid; (4) screening and preparing culture medium of hepialidae hirsutella sinensis and paecilomyces hepialid; and (5) controlling yield increase conditions. According to the method, at the adult stage, a high pressure mercury vapor lamp is used for trapping, so the imagoes of hepialus armoricanus around the lamp fly to the base, and the egg-laying amount is increased; ethanol, vinegar and sugar solution are sprayed on meadows beneficial to larva growth, so the larvae gather together; and the hepialidae hirsutella sinensis and the liquefied paecilomyces hepialid are sprayed on the gathering area of larvae to raise the infection rate of the larvae of hepialus armoricanus, so the aim of increasing the yield of cordyceps sinensis is achieved and the industrialized production is realized.

The invention relates to a method for producing a microbial strain, in particular to a method for producing a new variety of Hirsutella sinensis. In the invention, Hirsutella sinensis screened according to cell dynamic information acquired by rat macrophage culture is subjected to multistage culture and fermentation, then hyphae is filtered by a membrane with a molecular weight between 300 and 4,000 by a solid-liquid separation technique, the bacterial solution is dried, and thus, health-care food and medicines, which can improve an immune function, are manufactured. When the method is used, the instability of industrial Cordyceps sinensis strain is realized, digital standard control is applied in a whole process from strain screening to culture and fermentation to solid-liquid membrane treatment, and the standard production process of industrial Cordyceps sinensis is realized actually; and with an accurate screening process, high production speed, pure production process and low production cost, the method can be widely accepted by people.

The invention provides a disinsection fungi emulsifiable powder, comprising the following components: disinsection fungi spore powder or mycelium 40-60, vegetable oil 5-15, surfactant 5-10, stabilizer 5-10, dispersant 0.1-1; wherein the disinsection fungi is metarrhizium anisopliae, white muscardine fungi, paecilomyces, hirsutella or the mixture; the surfactant is nonionic surfactant, the vegetable oil is one kind or the mixture of many kinds of the oil according to arbitrary ratios selected from the palm oil, corn oil, soybean oil, cotton seed oil and colza oil; the stabilizer is one kind or the mixture of many kinds of the oil according to arbitrary ratios selected from the xanthan gum, carboxymethyl cellulose, gelatin; the dispersant is DW 1003, glucose ester of fatty acid or the mixture thereof. The storage and transportation of the disinsection fungi emulsifiable powder is convenient and the storage period of the fungi spore is effectively prolonged and the long-term storage is convenient and the preparation activity is higher; after application of the disinsection fungi emulsifiable powder, the oil can effectively absorb the ultraviolet ray and reduce the damage of the ultraviolet ray on the fungi spore, effectively increase the field control-effect stability. The process production technique of the disinsection fungi emulsifiable powder is simple and the disinsection fungi emulsifiable powder is easily mixed with the other compatible synergistic pesticide.

The invention provides a strain material adopting cordyceps sinensis mycoderma instead of the hirsutella sinensis spore bacterium as the cordyceps sinensis host infection bacterium. The strain material used for infecting hosts during cordyceps sinensis host raising is the cordyceps sinensis mycoderma. The strain material has the following beneficial effects: a method has the advantage of lower preparation cost of the strain material; and more importantly, by using the cordyceps sinensis mycoderma as the strain material, the survival rates and infection rates of the cordyceps sinensis hosts are obviously improved compared with the survival rates and infection rates of the hirsutella sinensis spore bacterium inoculated liquid and the host larvae.

The present invention discloses a fermenting production process of Hirsutella hepiali Chen & Shen for industrial purpose. It contains steps: a. Isolating new stain from original source; b. identifying whether the stain can grow stroma or not; c. culturing the strain in solid medium for rejuvenescence purpose; d. Secondly culturing the strain in liquid culture medium; e. fermenting. Said method provides a fermenting production process, which can continually identify whether the anamorphic fungi related to Chinese Cordyceps sinensis change or not, whether it retains the property of original strain or not. It also can be modified continually. According to these processes, the quality of the obtained product will be stable and the property will be retained stable for quite a long time. Therefore this method overcome the problems exist in the art that change of the strain, instability of quality of the product and so on.

The invention relates to an artificial culture method of cordyceps longissima sporocarp. A strain is cordyceps longissima agamotype-hirsutella longissima. The artificial culture method of the cordyceps longissima sporocarp includes A, cant tube strain activation culture; B, liquid shake flask seed culture; C, hypha stage culture; and D, sporocarp culture. The cordyceps longissima sporocarp cultured artificially has rodlike stroma and is solitary or branching and cinnamon, length is 1-7cm, the diameter is 1-3mm, a large amount of white coremium is grown at the top end or the side face of the stroma at later stage of sporocarp culture and is compact. The artificial culture method of the cordyceps longissima sporocarp is initiated, can perform large-scale culture of the cordyceps longissima sporocarp, solves the problem that wild cordyceps longissima sources are deficient, establishes a foundation for comprehensive development of cordyceps longissima edible value and industrialized production, provides technical support, and is simple in process, low in cost, wide in raw material source, non-toxic, free of pollution and capable of performing large-scale production.

The invention belongs to a method for cultivating cordyceps sinensis stroma, and particularly relates to a method for cultivating cordyceps sinensis stroma by using hirsutella sinensis. The method comprises the following steps: soaking maize pulp, soybean meal, wheat grains, rice grains, sorghum grains, silkworm chrysalis meal or crushed whole egg and beef peptone in water; mixing and filtering the soaked raw materials and auxin to obtain filter liquor; and inoculating the soaked materials to the hirsutella sinensis liquor, then cultivating the mixture in dark place, adding the filter liquor to the mixture, and cultivating the mixture in a greenhouse to obtain the cordyceps sinensis stroma. The method has the following advantages that: the asexual hirsutella sinensis strains are inoculated to a manually prepared culture medium so as to grow the stroma in batch, and the stroma can be cyclically grown. The biological conversion rate of the cultivated cordyceps sinensis stroma is about 40 percent, and the cordyceps sinensis stroma contains over 0.02 percent of adenosine C10H13N504 and over 0.10 percent of cordycepin C10H13N503.

The invention relates to IMP (inosine monophosphate) dehydrogenase synthesized by 'Bailing' producing fungus Chinese caterpillar fungus hirsutella sinensis to metabolize guanine nucleotide based on xanthosine monophosphate and coding genes and application of the IMP dehydrogenase. The IMP dehydrogenase comprises proteins shown by SEQ ID No.1, SEQ ID No.2 and SEQ ID No.3, and coding genes of the IMP dehydrogenase correspond to nucleotide sequences shown by SEQ ID No.4, SEQ ID No.5 and SEQ ID No.6. Detailed studies on metabolic pathways of the xanthosine monophosphate synthesizing the guanine nucleotide in principle are carried out and include that cloned DNAs (deoxyribonucleic acids) in the provided nucleotide sequences can be transferred into engineering bacteria by means of transduction, transformation and transconjunction, and high expressivity is given to the host guanine nucleotide by adjusting expressions of guanine nucleotide biosynthetic genes, so that an effective way for increasing output of the guanine nucleotide is provided, and the IMP dehydrogenase and the coding genes have significant application prospects.

The invention discloses a solid state fermentation method for cordyceps sinensis, which belongs to the technical field of fermentation. According to the method, corn, rice, rice husk, soybean oil, oyster shell and crab grass powder are adopted as components of a culture medium, prepared seeds are subjected to solid state fermentation, the culture medium is low in hardness and viscosity, the solid fermentation stirring problem is overcome, the culture medium is floppy and breathable, the growth speed of Chinese hirsutella sinensis is effectively increased, and cordyceps sinensis mycelia produced in a fermentation mode are non-toxic, free of environment pollution, environmental friendly and low in residue, and can be directly used as food production raw materials.

The invention relates to the field of microorganisms, and discloses a cordyceps sinensis fruiting body and a cultivating method thereof. The cultivating method includes the steps that hirsutella sinensis is inoculated onto a solid culture medium, fungal colony cultivation is performed for 35-55 days under the conditions that sunlight is kept out, the temperature is at 14-20 DEG C and the relative humidity is 45-60%, and then the fruiting body is induced under the conditions that the sunlight is kept out, the oxygen concentration is 10-15%, the temperature is 0-17 DEG C and the relative humidity is 70-90% until the fruiting body grows. According to the method, the hirsutella sinensis is inoculated onto the specific culture medium, the conditions of humidity, temperature and the like at different growth stages of the hirsutella sinensis are controlled, the real cordyceps sinensis fruiting body is cultivated in the low-temperature, lucifugal and oxygen-poor environment, and the immunomodulatory effect and the active material content of the cordyceps sinensis fruiting body are almost the same as those of a wild cordyceps sinensis fruiting body.

The invention provides a recycling method of hirsutella sinensis fermentation filtrate. The method includes: using the filtrate obtained by filtering the fermentation broth acquired by the fermentation culture of hirsutella sinensis as inlet water, performing electrodialysis treatment under a condition that water inlet flow is 200-400L / h and the operation voltage is 20-30V, collecting electrodialysis effluent liquid, namely the recycled water of the hirsutella sinensis fermentation filtrate, and using the recycled water to prepare microorganism culture media. The recycling method has the advantages that the electrodialysis equipment selected by the method is cheaper than imported equipment, and the salt rejection rate of the electrodialysis equipment can reach more than 95% under corresponding parameter setting; the water yield after the electrodialysis is about 90%; when the electrodialysis method is used to treat 1000L of fermentation filtrate, the energy consumption cost of the electrodialysis method is about 1.88 yuan per ton, and the equipment cost is about 0.5 yuan per ton; enterprise production cost can be evidently lowered when the electrodialysis method is used to treat the fermentation filtrate and the recycled water is used for preparing the culture media.

The invention relates to a preparation method for an Ophiocordyceps sinensis strain material and a method for infecting Thitarodes larvae with the Ophiocordyceps sinensis strain material. According to the methods, the principle that the imperfect stage of Ophiocordyceps sinensis--Hirsutella sinensis and plant tissue form a symbiont is employed; a prepared Ophiocordyceps sinensis strain source is inoculated onto the plant tissue; proper conditions are created to promote symbiosis of the Hirsutella sinensis and the plant tissue so as to activate infection activity of the Hirsutella sinensis on the host Thitarodes larvae; the activated Ophiocordyceps sinensis strain Hirsutella sinensis forms the fungus-plant symbiont with the plant tissue; and the fungus-plant symbiont is used as the strain material with high infection activity on the host Thitarodes larvae. The infection rate is up to 85.6 to 98.9% by directly feeding the Thitarodes larvae with the prepared strain material for infection.

A solid fermenting process of cordyceps features use of Hirsutella sinensis (CCTCC No.M87101) is used as the fermenting bacterial strain, and includes bacterial strain culture, solid fermenting culture, discharging mycelia, drying and pulverizing. Its advantages are high output rate (20-40%), no environmental pollution and low cost.

The invention discloses an original ecological separation method of cordyceps sinensis hirsutella sinensis. The original ecological separation method comprises a tissue separation method and an ascospore separation method. According to the method, seed sources are selected from fresh and ripening cordyceps sinensis in Yushu, Qinghai in China, and soil with the depth being 8 to 12 cm in a range with the diameter being 10 to 12cm around the sporocarp is remained during the collection; a culture medium is from living bodies of cordyceps sinensis host of swift moth larvae, white strong and living swift moth larvae in 4 to 5 age periods are selected, and sterile water is used for rinsing and storage and placing in time during the collection; the cordyceps sinensis and the living bodies of the cordyceps sinensis host of swift moth larvae are stored at the temperature being 0 to 4 DEG C for 0 to 15 days after the collection; the seed sources are transferred onto the culture medium for culture, and the parameters are controlled as follows during the culture: the temperature is 6 to 9 DEG C, the pH value is 6 to 6.5, the light illumination is 50 to 100lux, the air humidity is 55 to 60 percent, and the oxygen content is 20 percent.

The invention discloses an industrial fermentation production method of anamorph cordyceps sinensis, hirsutella sinensis Liu, Guo, Yu et Zeng, and especially relates to a production method of hirsutella sinensis bacterial powder. The method provided by the invention adopts different culture media at different culture phases, which not only ensures that dynamic nutrients are provided for normal growth of hirsutella sinensis, but also reduces the cost of industrial production, and ensures similar quality to natural cordyceps sinensis.

The invention relates to a health care beverage, and particularly discloses a caffeine-free health care juice beverage capable of effectively alleviating fatigue. The health care juice beverage comprises American ginseng, jujube, wolfberry fruit, Mycelium of Hirsutella hepiali Chen et Shen powder, white sugar, citric acid potassium sorbate, concentrated apple clarified juice and purified water. The health care juice beverage uses the Qi tonifying and cardiac functions of American ginseng, the blood nourishing and spleen invigorating functions of jujube, the sperm, liver and kidney replenishing function of wolfberry fruit, and the lung and kidney invigorating function of Mycelium of Hirsutella hepiali Chen et Shen powder similar to that of Cordyceps sinensis, and the medicines are used together, thereby being capable of realizing the efficacies of tonifying Qi and blood and nourishing five organs and having the health care function of relieving physical fatigue.

An oral liquid for sedation, promoting sleep, dilating trachea, lowering blood pressure and improving immunity is prepared from Chinese hirsutella sinensis through fermenting, centrifugal separation, filtering, filling it in containers, sterilizing and packing.

The invention relates to a preparation method of a tablet of hirsutella hepialid mycelia ultrafine powder. The preparation method mainly comprises the following steps of 1, crushing the cordyceps sinensis fungus hirsutella hepialid mycelia into 300 meshes of ultra-fine powder; 2, evenly mixing 300 meshes of powder with water to prepare slurry; 3, atomizing and drying the slurry into particles; 4, pelletizing; and 5, pressing into tablets. The technical problem of poor tabletting and molding effects caused by over-small particle size of ultrafine powder in the preparation process of the hirsutella hepialid mycelia ultrafine powder tablet is solved by chance by applying a common spray drying method in a traditional Chinese medicine processing process into the preparation of the hirsutella hepialid mycelia ultrafine powder tablet; and the unexpected technical effect is obtained.

The invention relates to a method for producing fermentative cordycep fungal powder by solid fermentation of mixed strains. The method comprises the following steps: carrying out slant culture, producing liquid strains, carrying out solid fermentation, and drying and grinding. The method is characterized in that the fermented strains are hirsutella sinensis, paecilomyces hepialid and cephalosporins. According to the method, in combination with the fermentation and growth characteristics of the hirsutella sinensis, the paecilomyces hepialid and the cephalosporins, solid fermentation is carried out on the three strains to prepare the fermentative cordycep fungal powder having ingredients closer to natural cordyceps sinensis and having more perfect effects; therefore, good economic benefits are brought to artificial cordyceps sinensis industrialization.

The invention discloses Hirsutella sinensis ZJB18002 and application thereof in production of cordycepic acid, and provides a novel slant medium for isolating Hirsutella sinensis, a biological identification method of Hirsutella sinensis, and a method to acquire a strain with 2-3 times higher yield of cordycepic acid through mutagenesis. An effective means to widen the biological application rangeof Hirsutella sinensis is provided, and the methods and the medium above have a major application prospect.

The invention discloses a Hirsutella hepiali Chen et Shen fungus which can be fermented at normal temperature, the fungus in systematics is named as (Hirsutella hepiali Chen et Shen)BS-1, and is mutagenized by the following method: protoplast is extracted from Hirsutella hepiali Chen et Shen strain which is separated from the stroma of the natural Cordyceps sinensis(Berk)Sacc., and the protoplast is irradiated by a 15-60W ultraviolet lamp at a place which is 10-50cm from the ultraviolet lamp for 1-300s, inoculated on MYG reproduction culture medium and cultured in a thermotank at the temperature of 25-28 DEG C; the mutagenized and cultured strains are inoculated in fermentation medium and acclimatized at the temperature of 25-28 DEG C for ten generations, and the contents of the active components are determined, the strains which grow well and have high strain powder yield and high contents of the active components are selected, and finally the Hirsutella hepiali Chen et Shen fungus strain which can be fermented at normal temperature is obtained. The invention also discloses a mutagenesis breeding method of the fungus and a fermentation process. The Hirsutella hepiali Chen et Shen strain can rapidly grow at normal temperature with short fermentation period and low production cost, so that the product has greater market competitiveness.

The invention discloses health capsules containing edible mushrooms and process for preparation, wherein the raw materials include ganoderma lucidum, Hirsutella hepiali Chen et Shen, hedgehog hydnum and schisandra fruit. The capsules have good effect in improving human immunity and protecting liver from chemical damages.

The present invention provides a set of cordycepin anabolism-related enzymes from bering producing strain cordyceps sinensis hirsutella sinensis-participated ADP ribose starting, genes encoding the enzymes, and application thereof. The related enzymes include proteins shown as SEQ ID No. 1-17, and the coding genes correspond to nucleotide sequences shown as SEQ ID No. 18-34. The invention conducts detailed researches on the principle of metabolic pathways of cordycepin synthesis from bering producing strain cordyceps sinensis hirsutella sinensis ADP ribose. The cloned DNA of the nucleotide sequences provided by the invention can be transferred into engineering bacteria by a transduction, transformation and conjugal transfer method. By adjusting the expression of cordycepin biosynthesis genes, high expression of cordycepin for the host is given.

Compositions comprising Hirsutella sinensis mycelia extracts and chromatographically separated polysaccharide-enriched fractions thereof are provided. Methods for extracting Hirsutella sinensis mycelia are provided. Compositions for methods for their use in amelioration, prevention and treatment of sepsis, acute endotoxemia and inflammatory responses are disclosed.

The invention provides a method for preparing a cordyceps polysaccharide biological preparation, and relates to a preparation method of preparations. The method comprises the following steps: preparing a cordyceps taishanensis culture medium: culturing cordyceps taishanensis, coarsely extracting cordyceps polysaccharide, purifying cordyceps polysaccharide; molding, namely cutting bitter gourd, fagopyrum tararicum, radix ophiopogonis, cyclocarya paliurus, alkekengi and purslane into dices, baking at 60 DEG C, and crushing; sufficiently mixing the following raw materials in percentage by weight: 30 percent of cordyceps polysaccharide, 25 percent of alkekengi, 15 percent of cyclocarya paliurus, 10 percent of fagopyrum tararicum, 10 percent of bitter gourd, 5 percent of radix ophiopogonis and 5 percent of purslane; combining medicinal auxiliaries to prepare the preparatin. According to the cordyceps polysaccharide biological preparation, cordyceps polysaccharide extracted from artificially cultured hirsutella sinensis mycelium is adopted as a main raw material, and is compatible with alkekengi, cyclocarya paliurus, fagopyrum tararicum, bitter gourd, radix ophiopogonis and purslane, so that the blood glucose reducing effect of the biological preparation is better that that of single cordyceps polysaccharide. The cordyceps polysaccharide biological preparation can be used for comprehensively reducing blood glucose, does not have toxic or side effect, is low in price, and is suitable for large-scale production.

The invention provides a chitinase A which comes from a corbrin-capsule-producing strain cordyceps sinensis hirsutella sinensis and participates in hydrolysis of colloid chitin for generating N-acetyl-D-glucosamine, a gene encoding the enzyme, and application of the enzyme and the gene. The amino acid sequence of chitinase A is shown as SEQ ID No. 1, and the sequence of the encoding gene is shown as SEQ ID No. 2. A cloned DNA of the provided nucleotide sequence can be transferred into an engineering bacterium through a transduction, transformation or conjugal transferring method. By adjusting chitin hydrolysis and expression of the N-acetyl-D-glucosamine synthetic gene, a host is endowed with property of highly expressing chitinase A, an effective approach is provided for expanding biological application of chitinase A, and important application prospect is provided.