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Production method of hirsutella sinensis bacterial powder

A Chinese Mortierella production method technology, applied in the production field of Chinese Mortierella powder, can solve the problems of high price, low dry matter yield, low mannitol and adenosine content, etc., and achieve the improvement of the content of main nutrients , Wide range of raw material sources, low production cost

Active Publication Date: 2014-04-16
青海珠峰冬虫夏草工程技术研究有限公司
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  • Abstract
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Problems solved by technology

[0004] This patent only discloses the formula of slant medium and fermentation medium. The market price of silkworm chrysalis as the main raw material of the medium is 16-18 yuan / kg, which is expensive, resulting in high cost of industrial production of Cordyceps sinensis powder
Moreover, the fermentation process disclosed in this patent basically followed the fermentation production method of Chinese Cordyceps sinensis fungus disclosed in Chinese patent CN97110448.4 and the large-scale fermentation production of Cordyceps sinensis disclosed in CN200410101728. The process follows the traditional antibiotic fermentation process. There is no dynamic supply of nutrients during the fermentation of Cordyceps sinensis living bacteria Trichosporum sinensis, so that the strains are not completely converted, resulting in a low yield of dry matter after fermentation. The main components of the product are total amino acids, mannitol and low adenosine

Method used

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  • Production method of hirsutella sinensis bacterial powder

Examples

Experimental program
Comparison scheme
Effect test

Embodiment 1

[0020] First take broad bean powder 2%, glucose 5%, corn flour 1.5%, peptone 1%, agar 0.75%, bran 0.5%, magnesium sulfate 0.05%, dipotassium hydrogen phosphate 0.1%, Water 89.1% makes the slant culture medium, and after receiving Mortierella sinensis strain, ferment for 30 days at 13 DEG C, then the strain is inoculated in broad bean powder 2%, glucose 2.5%, corn flour by weight volume ratio (g / ml) 1%, peptone 2%, yeast extract 5%, magnesium sulfate 0.05%, dipotassium hydrogen phosphate 1%, water 86.95% on a medium shaker, fermented at 17°C for 7 days, and then inoculated in the first-level seed tank, the medium According to weight volume ratio (g / ml), glucose 2%, yeast extract 0.5%, peptone 1%, magnesium sulfate 0.1%, dipotassium hydrogen phosphate 0.8%, water 95.6%, fermented at 13°C for 9 days, and then transferred to a container Fermented at 13 DEG C for 9 days in the secondary fermentation tank of the same level of fermentation medium, and then transferred to a container ...

Embodiment 2

[0022] First take broad bean powder 3%, glucose 4%, corn flour 1%, peptone 0.5%, agar 1%, bran 4%, magnesium sulfate 0.1%, dipotassium hydrogen phosphate 0.05%, Water 86.35% makes slant culture medium, and connects Mortierella sinensis strain 17 ℃ of fermentations for 20 days, then inoculates bacterial classification in by weight volume ratio (g / ml) with broad bean powder 3%, glucose 4%, corn flour 4%, 0.5% peptone, 1% yeast extract, 0.1% magnesium sulfate, 0.05% dipotassium hydrogen phosphate, and 87.35% water on a medium shaker, fermented at 13°C for 9 days, and then inoculated in the first-level seed tank. According to weight volume ratio (g / ml), glucose 3%, yeast extract 4%, peptone 0.5%, magnesium sulfate 1%, dipotassium hydrogen phosphate 1%, water 90.5%, fermented at 17°C for 7 days, and then transferred to a container 17 DEG C of fermenting 7 days in the secondary fermentor of same level fermentation medium, transfer to again containing broad bean powder 3%, glucose 4%...

Embodiment 3

[0024]First take broad bean powder 4%, glucose 2.5%, corn flour 4%, peptone 2%, agar 0.5%, bran 1%, magnesium sulfate 0.8%, dipotassium hydrogen phosphate 1%, Water 84.2% makes the slant culture medium, and after connecting the Mortierella sinensis strain, ferment for 22 days at 15°C, then the bacterial strain is inoculated in the weight-volume ratio (g / ml) with broad bean flour 4%, glucose 5%, corn flour 3%, peptone 1%, yeast extract 2%, magnesium sulfate 0.9%, dipotassium hydrogen phosphate 0.2%, water 83.9% on a medium shaker, fermented at 14°C for 8 days, and then inoculated in the first-level seed tank, the medium According to weight volume ratio (g / ml), glucose 4%, yeast extract 3%, peptone 1.5%, magnesium sulfate 0.05%, dipotassium hydrogen phosphate 0.1%, water 91.35%, fermented at 15°C for 8 days, and then transferred to a container 15 DEG C of fermenting 8 days in the secondary fermenter of same grade fermentation medium, transfer to again containing broad bean flour...

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Abstract

The invention discloses an industrial fermentation production method of anamorph cordyceps sinensis, hirsutella sinensis Liu, Guo, Yu et Zeng, and especially relates to a production method of hirsutella sinensis bacterial powder. The method provided by the invention adopts different culture media at different culture phases, which not only ensures that dynamic nutrients are provided for normal growth of hirsutella sinensis, but also reduces the cost of industrial production, and ensures similar quality to natural cordyceps sinensis.

Description

technical field [0001] The invention relates to a production method of Mortierella sinensis (Hirsutella sinensis Liu, Guo, Yu et Zeng), an anamorph of Cordyceps sinensis fungus (Cordyceps sinensis), by industrialized fermentation, in particular to a production method of Mortierella sinensis fungus powder. Background technique [0002] Cordyceps sinensis is a complex of insects and bacteria formed after the larvae of Hepialus armoricamus Oberthur of the Lepidoptera genus Hepialus armoricamus are infected with the fungus Mortierella chinensis, and the complex is formed after a large number of reproductions in the body, that is, the larvae of the genus Hepialus armoricamus Oberthur. It reproduces continuously in its body until it dies and becomes a zombie, and finally grows a sub-seat to form a natural Cordyceps sinensis. From the perspective of the formation process of natural Cordyceps sinensis, Trichosporum sinensis continuously absorbs nutrients through the larvae during th...

Claims

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Application Information

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IPC IPC(8): C12P1/02C12R1/645
Inventor 王辉
Owner 青海珠峰冬虫夏草工程技术研究有限公司
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