526 results about "Yeast extract" patented technology

The invention relates to a concrete anti-cracking self-repairing agent in the technical field of building materials, and aims to solve the problem that concrete cracks are difficult to self-repair. According to the technical scheme, the self-repairing agent is characterized by comprising a microbial repairing unit formed by ZSM-5 type zeolite molecular sieve loaded bacillus pasteurii spores, mesoporous silica particles loaded with L-calcium aspartate and Fe3O4 (at) SiO2 core-shell nanoparticles which are fixed through an in-situ polymerization coating layer to form a magnetic thermal response capsule layer coating the surface of the microbial repairing unit, pLGA microspheres containing yeast extract are positioned on an interface of zeolite and hydrogel, and a trehalose-anhydrous calcium chloride eutectic compound is used as a directional mineralization accelerant. The concrete anti-cracking self-repairing agent provided by the invention is mainly used for intelligent identification and self-repairing of concrete cracks, and efficient repairing is realized through a microbial mineralization, calcium ion slow release and magnetic thermal response synergistic mechanism.

The invention relates to the technical field of environmental restoration, in particular to a slow-release material for synergistically restoring heavy metal and organic compound contaminated soil based on modified biochar and microorganisms and an application method thereof.The slow-release material is prepared from, by weight, 50-80 parts of modified biochar, 30-40 parts of sodium alginate, 10-20 parts of gelatin, 5-10 parts of glycerin and 3-5 parts of calcium carbonate. The multi-modified biochar is prepared from, by weight, 300-400 parts of a functional flora mixed solution and 5-10 parts of mixed nutrient elements of glucose, yeast extract and KHPO, and through the synergistic effect of the multi-modified biochar and the functional flora, efficient immobilization (the immobilization rate gt is 60%) of heavy metal (lead, cadmium and chromium) and efficient degradation (the degradation rate gt is 70%) of organic pollutants (polycyclic aromatic hydrocarbon and petroleum hydrocarbon) are achieved.

The invention relates to the field of nutrient solutions, and particularly discloses a high-calcium-zinc dietary nutrient solution for promoting skeletal development and a preparation method thereof, the high-calcium-zinc dietary nutrient solution for promoting skeletal development comprises the following raw materials: calcium citrate, zinc citrate, a zinc-containing yeast extract, vitamin D3, a nutrition enhancer, functional oligosaccharide, xanthan gum, anhydrous citric acid, essence, a sweetening agent and water; the preparation method comprises the following steps: mixing the zinc citrate, the zinc-containing yeast extract, the seaweed meal, the natto powder, the stachyose and the trehalose to prepare a mixture A; uniformly premixing stevioside, xanthan gum and trehalose to obtain a mixture B; s4, vitamin D3 powder and trehalose are premixed and dissolved, and a mixture C is prepared; heating the residual water, adding the mixture A, the fructo-oligosaccharide, the anhydrous citric acid, the calcium citrate, the mixture B, the trehalose and the mixture C, adding the essence, and stirring to obtain the beverage. The preparation method has the characteristic of preparing the liquid calcium-zinc dietary nutrient solution with good absorption and utilization rate and palatability.

The application relates to an organic-inorganic compound fertilizer containing functional microorganisms and a production method thereof, and relates to the technical field of fertilizers. The fertilizer comprises an inorganic core layer, an organic coating layer and a functional microorganism outer layer. The functional microorganism outer layer comprises the following components in mass fractions: 10-15 parts of compound functional microorganisms, 5-10 parts of synergistic growth-promoting microorganisms, 2-3 parts of trehalose, 1-2 parts of mannitol, 10-15 parts of sodium alginate, 8-10 parts of chitosan, 2-4 parts of yeast extract and 30-40 parts of modified active carbon carriers. The production method comprises the following steps: preparing the inorganic core layer, preheating the inorganic core layer, spraying the organic coating layer material to coat the surface, and obtaining a preliminary product with the organic coating layer; coating the functional microorganism outer layer material on the surface of the organic coating layer of the preliminary product, low-temperature drying and screening, and obtaining the organic-inorganic compound fertilizer containing the functional microorganisms. The application has the effects of improving the slow-release effect of the fertilizer and the survival rate of the microorganisms.

The invention provides schizochytrium limacinum FJ-1 and application thereof in production of phospholipid type EPA (eicosapentaenoic acid). The application comprises the following steps: taking a cryopreserved schizochytrium limacinum strain, and inoculating the schizochytrium limacinum strain into a liquid seed culture medium for pre-culture; the method comprises the following steps: inoculating pre-cultured schizochytrium limacinum into a fermentation tank according to the inoculum size of 5-10% v / v, culturing for 48-60 hours at the sea salt concentration of 0.5-1% w / v, fermenting for 48-72 hours, supplementing sea salt to a culture medium in batches until the sea salt concentration is 3-5% w / v, and continuously culturing for 96-120 hours by controlling the glucose concentration and the yeast extract concentration through supplementing materials. The technical problem that the synthesis efficiency of schizochytrium limacinum phospholipid type EPA is low is solved through precise regulation and control of salinity stress, an efficient, economical and generalizable innovative scheme is provided for industrial production of functional lipid, and the method is suitable for large-scale production.

The invention relates to the technical field of bacterium enrichment nutrient solutions, in particular to a bacterium enrichment nutrient solution for fermentation and a preparation method of the bacterium enrichment nutrient solution. The culture medium is prepared from the following raw materials in parts by weight: maltodextrin, crystalline glucose, low-pigment yeast extract powder, refined soy peptone, dipotassium phosphate, monopotassium phosphate, magnesium sulfate heptahydrate, sodium chloride, phytic acid, L-ascorbic acid, manganese sulfate monohydrate, biotin, thiamine hydrochloride and choline chloride. Through a double-carbon-source structure, maltodextrin and crystalline glucose cooperate, metabolic oscillation is avoided, and the energy utilization rate is increased; the low-pigment yeast extract powder and the refined soy peptone are complementary, so that the rapid growth requirement of thalli is met; phytic acid is chelated with metal ions to prevent precipitation, L-ascorbic acid is used for resisting oxidation, and biotin and thiamine activate a coenzyme pathway to realize accurate regulation and control of a metabolic network.

The invention belongs to the technical field of edible flavors and fragrances, and particularly relates to a method for preparing bidirectional controlled-release meat flavor by using bean dreg protein. The method comprises the following steps: taking bean dreg protein as a raw material, mixing bean dreg protein zymolyte, yeast extract, amino acid or peptide, reducing sugar and grease, carrying out three-stage heating, and respectively mixing the three-stage heating products according to a specific ratio. The obtained essence has the characteristic of bidirectional controlled-release fragrance, is pure, strong, mellow and vivid in meat flavor, can be well applied to food seasonings, and has a wide application prospect.

The invention discloses an improved self-induction culture medium and a culture process, and belongs to the technical field of bioengineering. The improved self-induction culture medium is prepared from 10 g / L of glucose, 3-5 g / L of glycerin, 3 g / L of lactose, 24 g / L of yeast extract, 8 g / L of casein hydrolysate, 0.02-0.05 mM of malonic acid, 1 mM of methionine and 1-3 mM of MgSO4, a phosphate buffer solution is added to control the pH to be 7.2, and the improved self-induction culture medium is prepared by mixing after sterilization. Cells are inoculated to an improved self-induction culture medium for culture, automatic switching of growth-induction stages can be realized based on a pH and DO linkage dynamic feeding control method, the operation complexity is reduced, and the controllability is higher. The culture medium does not need a special inducer, cost is reduced, and protein expression quantity is improved.

To provide a topical skin preparation that effectively promotes collagen production within the skin, exhibits excellent usability, and effectively enhances post-use stratum corneum moisture content.SOLUTION: A topical skin preparation comprises niacinamide, a heparin-like substance, dipotassium glycyrrhizinate, a collagen peptide, a yeast extract, and glycylglycine. It is preferable that the topical skin preparation further contains hydrogenated soybean phospholipids and phytosterols. It is more preferable that the topical skin preparation has a niacinamide content of 5 mass% or more. It is also preferable that the topical skin preparation further contains at least one plant extract selected from Arnica montana and Plantago major.SELECTED DRAWING: Figure 1

This invention provides a novel method for preparing a medical collagen sponge, relating to the field of medical sponge technology. The invention uses yeast extract obtained by Soxhlet extraction of brewer's yeast as a raw material, which is mixed and reacted with polyvinyl alcohol and graphene oxide to obtain the novel medical collagen sponge. The medical collagen sponge prepared by this invention exhibits excellent performance and good hemostatic effect. Furthermore, the preparation method is simple, and it degrades postoperatively, demonstrating significant application value in the field of medical materials.

The invention discloses a method for improving the fermentation performance of clostridium aerovorans and application of the clostridium aerovorans, and according to the method, an effective amount of methionine or a derivative thereof is added into a fermentation culture medium of the clostridium aerovorans to completely replace expensive complex organic nitrogen sources such as yeast extracts. The method successfully solves the three technical bottlenecks of slow biomass accumulation, low ethanol yield and dependence on expensive nitrogen sources in the fermentation process of the clostridium aerovorans by utilizing synthesis gas or CO2 / H2 mixed gas. Experiments prove that methionine or a derivative thereof can synergistically promote growth metabolism of thalli and a synthesis path of a product, so that the gas conversion efficiency of a carbon source is remarkably improved and metabolism is guided to flow to ethanol synthesis while the growth rate and the final biomass of the thalli are improved, and thus the production capacity of alcohols is synchronously enhanced. The method is simple in process and remarkable in effect, and an efficient and economical new strategy is provided for reducing the production cost of biofuel and promoting industrial application of a synthesis gas biorefinery technology.

The invention relates to the technical field of pet food, in particular to flavoring agent powder for pet dogs and a preparation method of the flavoring agent powder. Comprising the following raw materials: 40 parts of beef liver, 10 parts of hairtail, 7-40 parts of ricefield eel, 1 part of protease, 0.5-1 part of lipase, 0.5-1 part of flavor enzyme, 5-10 parts of saccharides, 10 parts of fish oil, 5-10 parts of maltodextrin, 0.5-1 part of sodium hydroxide, 5 parts of phosphoric acid, 3 parts of amino acid, 2 parts of thiamine, 1 part of yeast extract and 0.5-1 part of lactoferrin. The dog feed can increase the appetite of dogs from the sense of smell, maintain the health balance of intestinal flora of dogs and improve the micro-ecological environment of intestinal tracts.

The invention relates to the technical field of microbial fermentation, in particular to a fermentation medium for producing oxytetracycline. According to the fermentation culture medium, a corn starch-molasses-wheat hydrolyzed sugar composite carbon source, a soybean cake powder-corn steep liquor-yeast extract composite nitrogen source and a choline chloride-betaine-L-lysine precursor accelerant in a specific proportion are used as a core innovative system, and a form regulating agent, a defoaming agent and inorganic salt are cooperatively added. Through the synergistic effect of multiple components, the fermentation titer and yield of oxytetracycline are remarkably improved, the fermentation period is shortened, the generation of heteroacid is effectively inhibited, the mycelium form is optimized, and the oxytetracycline fermentation medium is suitable for industrial large-scale production of oxytetracycline.

The present invention relates to the field of biotechnology, and particularly to a preparation method of yeast extract rich in betaine. The present invention provides a preparation method of yeast extract rich in betaine. Among them, the yeast extract rich in betaine includes protein, amino acids, peptides, nucleotides, and betaine. The mass percentage content of protein is 50% - 75%, and the mass percentage content of betaine is 0.2% - 4%. In the present invention, yeast rich in betaine is obtained by the yeast absorbing and enriching betaine in the betaine-rich carbon source during the growth process, and then the yeast extract rich in betaine is obtained through enzymatic hydrolysis and refinement. This technology makes up for the blank of yeast extract of betaine, and is mainly involved in the fields of food, health food, feed, medicine, etc.

The invention discloses a fermentation process for shortening a fermentation period and efficiently synthesizing Ectoine. The fermentation process comprises the following steps: screening and activating high-yield strains, preparing a fermentation culture medium, performing fermentation culture, performing sectional dissolved oxygen regulation and control, performing double-stage pH dynamic regulation and control, performing carbon-nitrogen ratio dynamic regulation based on metabolic flow analysis, performing osmotic pressure gradient induction in the later stage of fermentation, and extracting and purifying a product. According to the fermentation process for shortening the fermentation period and efficiently synthesizing the Ectoin, the expression quantity of key enzymes for synthesizing the Ectoin is increased by 80% or above compared with that of an original strain through the Halomonassp.ECT-09 strain bred through ultraviolet-nitrosoguanidine compound mutation, the basic yield reaches 35 g / L, a foundation is laid for high yield, and the method is suitable for industrial production. A glucose-trehalose mixed carbon source and a yeast extract-ammonium nitrate mixed nitrogen source are adopted, and an L-aspartic acid-L-asparagine mixed precursor is added, so that the metabolic pathway is shortened, and the loss of an intermediate product is reduced.

The invention relates to the technical field of fermented beverages, in particular to a preparation method of a high-activity fruit and vegetable probiotic fermented beverage, which comprises the following steps: adding composite fruit and vegetable juice after enzymolysis, mineral water, xylooligosaccharide, grape seed extract, yeast extract and xanthan gum into a fermentation tank, and stirring until the materials are completely dissolved; then carrying out sterilization treatment by adopting high-temperature instantaneous sterilization; adding probiotic microcapsules into the sterilized mixed liquid, and performing staged temperature-controlled fermentation to obtain fermentation liquid; and performing homogenization treatment on the fermentation liquid, and finally performing vacuum degassing to obtain the high-activity fruit and vegetable probiotic fermented beverage. A composite wall material (sodium alginate-eucheuma powder-gellan gum) of the probiotic microcapsule forms a compact gel network to effectively isolate phenols, organic acids and other antibacterial components in fruits and vegetables, so that the damage of pH fluctuation and temperature change to thalli is resisted.

The invention discloses a sports nutrition solid food for supplementing protein and a preparation method, and belongs to the technical field of foods.The sports nutrition solid food is prepared from, by weight, 31-60 parts of compound amino acid powder, 10-15 parts of isomaltitol, 1-6 parts of taurine, 1-5 parts of Chinese yam peptide, 5 parts of glycine, 5 parts of gamma-aminobutyric acid, 5 parts of L-lysine hydrochloride and 1 part of yeast protein powder. The formula comprises the following components in parts by weight: 1-3 parts of whey protein powder, 1-2 parts of hydroxymethyl propyl cellulose, 0.1-1 part of yeast extract, 0.1-1 part of yeast beta-glucan, 2-6 parts of bovine colostrum powder, 0.5 part of stevioside, 0.1 part of zinc glycinate, 0.1 part of ferrous lactate, 0.5 part of essence and 2-3 parts of magnesium stearate. The compound amino acid powder, the Chinese yam peptide, the glycine, the L-lysine hydrochloride, the yeast protein powder, the whey protein powder, the yeast extract and the bovine colostrum powder are compounded, so that protein consumed by a human body after exercise can be comprehensively supplemented.

The invention provides a beef essence preparation method and system based on dual enzymolysis-Maillard reaction synergistic interaction. The preparation method comprises the following steps: (1) carrying out first-step enzymolysis, namely adding compound protease into minced beef, regulating the pH value of a system to 6.5-7.0, controlling the temperature to be 45-50 DEG C, and carrying out enzymolysis reaction for 2-3 hours; (2) carrying out second-step enzymolysis, adding lipase derived from aspergillus oryzae, adjusting the pH value of the reaction system to 7.5-8.0, raising the temperature to 55-60 DEG C, and carrying out enzymolysis reaction for 1-2 hours; (3) performing enzyme deactivation treatment to obtain enzymatic hydrolysate; and (4) mixing the enzymatic hydrolysate with reducing sugar, thiamine and a yeast extract, and carrying out a Maillard reaction. The beef ingredients can be fully decomposed through double enzymolysis, and sufficient flavor precursor substances are provided for reaction; the Maillard reaction is intelligently regulated and controlled, more flavor substances are generated, and the flavor of the essence is remarkably improved.

The invention relates to the technical field of extraction of rare earth elements by a biological method, and provides a biological leaching method for extracting rare earth elements in fly ash by using aspergillus niger, which comprises the following steps: adding fly ash into a culture medium, sterilizing, inoculating aspergillus niger spore suspension, culturing, filtering leachate, and drying to obtain the rare earth elements, the culture medium consists of the following components in percentage by weight: 1-2% of glucose, 0.5-1.5% of a nitrogen source and the balance of water, the nitrogen source comprises peptone, yeast extract powder and diammonium hydrogen phosphate; the mass ratio of the peptone to the yeast extract powder to the diammonium hydrogen phosphate is 5: 1: (0.05-0.5). Through the technical scheme, the problem of low extraction rate of rare earth elements in related technologies is solved.

The invention relates to the technical field of skin care product raw materials, and particularly discloses a composition for controlling oil and removing acne and application thereof. The composition for controlling oil and removing acne comprises the following components in parts by mass: 3.2478-5.413 parts of a humectant; 3-5 parts of a penetration enhancer; 0.0072 to 0.012 part of a preservative; 3.1164 to 5.194 parts of a composite extract product; 2.6286 to 4.381 parts of a solvent; the composite extract is prepared by compounding a lotus corniculatus seed extract, a yeast extract, a rosa multiflora flower extract and a parsley flower extract. The acne-removing mask has the advantage of further improving the acne-removing effect.

The present invention belongs to the field of biopharmaceuticals and specifically relates to a composition for detoxifying alcohol and preventing hangovers and its preparation method, comprising the following steps: 1) adding a portion of yeast extract to purified water to obtain solution A, adding curcumin to anhydrous ethanol to obtain solution B, and mixing solution A and solution B to obtain a mixed solution; 2) adjusting the pH of the mixed solution to 5-6; 3) placing the mixed solution in a -25°C cold trap and allowing it to stand for 16-32 hours until crystals precipitate; 4) filtering at 4°C to obtain a filter cake and a filtrate; 5) pulverizing the filter cake to obtain a crystalline powder; and 6) uniformly mixing the crystalline powder, another portion of yeast extract, dihydroquercetin, and excipients, and then tableting to obtain the composition for detoxifying alcohol and preventing hangovers. The present invention utilizes a complex formed by precipitating the yeast extract and curcumin cocrystals, which expands the application range of the composition and improves the solubility and bioavailability of curcumin.

The invention discloses a brevundimonas sp. Fermentation medium and application thereof in prevention and treatment of plant root-knot nematodes. Each liter of the brevundimonas chinensis fermentation medium comprises the following components by mass or volume: 6-10 g / L of yeast powder, 16-18 g / L of peptone, 31-45 ml / L of nematode lysis buffer, 0.8-2.5 g / L of chitin and 5.6-6 g / L of yeast extract, and the pH value of the brevundimonas chinensis fermentation medium is 6.5-6.8. The application of the fermentation broth culture medium refers to the application of the obtained fermentation broth in prevention and treatment of plant root-knot nematodes. According to the present invention, with the fermentation medium, the fatality rate of the obtained fermentation broth on the plant root-knot nematode exceeds 95%, the action time does not exceed 12 h, the nematode can be rapidly killed before the larva enters the plant, and the good application prospect is provided in the plant root-knot nematode prevention and control.

The invention discloses a culture medium for identifying gardnerella vaginalis, and relates to the technical field of bacterial culture media, the key points of the technical scheme are as follows: the culture medium comprises the following raw material components by concentration: 10.0 g / L of casein pancreatin digest, 3.0 g / L of cardiac pancreatin digest, 5.0 g / L of yeast extract powder, 1.0 g / L of corn starch, 5.0 g / L of sodium chloride and 13.0 g / L of agar, and the final concentration of defiberized sheep blood is 5%. The pharmaceutical composition is prepared from 1 mg / mL of sodium alginate, 1 mg / mL of sodium alginate, 1 mg / mL of polyanethole Sulfonate (SPS) and 10 [mu] g / mL of vancomycin. According to the invention, through optimal combination of specific components and dosages thereof, rapid identification and screening of gardnerella vaginalis can be realized, and the method has the advantages of accurate result, strong specificity and the like, and has practical popularization and application values.

The invention belongs to the technical field of food processing, and particularly discloses a preparation process of natural preservative chicken soup based on lactobacillus fermentation. The process mainly comprises the following steps: decocting chicken and water according to a specific ratio to obtain basic chicken soup; the preparation method comprises the following steps: adding lactose, a yeast extract and the like as fermentation substrates, sterilizing, and inoculating a compound lactic acid bacteria starter consisting of lactobacillus plantarum and pediococcus pentosaceus to carry out anaerobic directional fermentation until the pH value is reduced to 4.0-4.6; and after fermentation is completed, adding natural preservative enhancers such as lactoferrin, and finally, homogenizing and filling to obtain the product. Natural preservation and flavor improvement of the chicken soup are synchronously achieved through a biological fermentation method, the obtained product is high in bacteriocin yield and remarkable in pathogenic bacterium inhibition effect, long-acting preservation can be achieved without adding chemical preservatives, meanwhile, the content of free amino acid and umami substances is greatly increased, and the fresh-keeping effect is good. The method is suitable for industrial production of high-quality concentrated chicken soup seasoning and liquid soup base.

The present application relates to a kind of skin care oil compositions for sensitive skin damage repair and its application, the skin care oil compositions include microbial oil and base oil, the microbial oil is with perilla seed powder, almond powder, camellia seed powder, glucose, protein peptone, yeast extract as substrate, using schizochytrium and lactobacillus plantarum to ferment substrate, the microbial oil obtained by fermentation has good effect of repairing skin damage.

The invention belongs to the technical field of hotpot condiments, and particularly relates to a bovine bone marrow oil mellow and hot hotpot condiment and a preparation method thereof. Comprising the following raw materials in parts by weight: 205 to 298 parts of edible beef tallow, 9.5 to 14 parts of edible bovine bone marrow oil, 15 to 25 parts of dried chili, 18 to 26 parts of chafing dish bean halves, 4 to 6 parts of fermented soya beans, 13 to 20 parts of monosodium glutamate, 10 to 16 parts of edible salt, 7 to 12 parts of fresh ginger, 9 to 14 parts of garlic, 7 to 11 parts of shallots, 10 to 16 parts of spices, 4.5 to 7 parts of yeast extract, 3 to 4.5 parts of white granulated sugar, 0.5 to 0.97 part of disodium 5 '-ribonucleotide, 15.8 to 23.8 parts of edible flavors and fragrances and 0.5 to 0.9 part of capsanthin. The edible bovine bone marrow oil in the mellow and hot hotpot condiment product containing the bovine bone marrow oil has rich and deep animal fat fragrance, the fragrance is mellower than beef tallow, the condiment is good in mellowness, and the flavor and taste are improved.

This invention discloses a processing technology for antibody-enhancing probiotic feed, which includes raw material pretreatment, substrate preparation, sterilization, cooling, strain activation, aseptic inoculation, constant-temperature anaerobic fermentation, immune-enhancing compounding, filtration and homogenization, and aseptic filling and storage. The fermentation substrate liquid includes purified water, molasses, yeast extract, compound trace elements, and buffer salts. A compound microbial community composed of *Lactobacillus plantarum*, *Bacillus subtilis*, *Bifidobacterium*, and yeast is used for fermentation. The temperature is controlled at 35–39°C during fermentation, and intermittent stirring is employed. After fermentation, astragalus polysaccharide, echinacea extract, and trehalose are added for compounding. This invention reduces the loss of active ingredients by adding immune-enhancing components after fermentation, and improves the survival rate of probiotics and fermentation stability through intermittent stirring. The resulting liquid feed has a high live bacteria content and immune-enhancing effect.

The invention relates to the technical field of beverage processing, in particular to a corn juice compound beverage and a preparation method thereof. The feed comprises the following raw materials: corn, black rice, oat, carrots, corncob powder, orange peel, compound enzyme, beta-cyclodextrin, Arabic gum, inulin, a yeast extract, monopotassium phosphate, vitamin B and compound strains. The compound enzyme seed is prepared from the following components: alpha-amylase, pullulanase, tannase and neutral protease; the composite strain is prepared from the following components: saccharomycetes, lactobacillus plantarum and monascus purpureus. According to the corn juice composite beverage and the preparation method thereof, through multi-dimensional synergistic interaction, the beverage realizes a layering flavor with both freshness, fruity flavor and cereal sweetness and fragrance, an anti-oxidation network constructed by polyphenol, carotenoid and ferulic acid and an intestinal tract nursing effect brought by complementation of soluble-insoluble fibers form the corn juice composite beverage with functional value and sensory experience.