342 results about "Yeast extract" patented technology

The invention relates to the technical field of environmental restoration, in particular to a slow-release material for synergistically restoring heavy metal and organic compound contaminated soil based on modified biochar and microorganisms and an application method thereof.The slow-release material is prepared from, by weight, 50-80 parts of modified biochar, 30-40 parts of sodium alginate, 10-20 parts of gelatin, 5-10 parts of glycerin and 3-5 parts of calcium carbonate. The multi-modified biochar is prepared from, by weight, 300-400 parts of a functional flora mixed solution and 5-10 parts of mixed nutrient elements of glucose, yeast extract and KHPO, and through the synergistic effect of the multi-modified biochar and the functional flora, efficient immobilization (the immobilization rate gt is 60%) of heavy metal (lead, cadmium and chromium) and efficient degradation (the degradation rate gt is 70%) of organic pollutants (polycyclic aromatic hydrocarbon and petroleum hydrocarbon) are achieved.

The application relates to an organic-inorganic compound fertilizer containing functional microorganisms and a production method thereof, and relates to the technical field of fertilizers. The fertilizer comprises an inorganic core layer, an organic coating layer and a functional microorganism outer layer. The functional microorganism outer layer comprises the following components in mass fractions: 10-15 parts of compound functional microorganisms, 5-10 parts of synergistic growth-promoting microorganisms, 2-3 parts of trehalose, 1-2 parts of mannitol, 10-15 parts of sodium alginate, 8-10 parts of chitosan, 2-4 parts of yeast extract and 30-40 parts of modified active carbon carriers. The production method comprises the following steps: preparing the inorganic core layer, preheating the inorganic core layer, spraying the organic coating layer material to coat the surface, and obtaining a preliminary product with the organic coating layer; coating the functional microorganism outer layer material on the surface of the organic coating layer of the preliminary product, low-temperature drying and screening, and obtaining the organic-inorganic compound fertilizer containing the functional microorganisms. The application has the effects of improving the slow-release effect of the fertilizer and the survival rate of the microorganisms.

The invention provides schizochytrium limacinum FJ-1 and application thereof in production of phospholipid type EPA (eicosapentaenoic acid). The application comprises the following steps: taking a cryopreserved schizochytrium limacinum strain, and inoculating the schizochytrium limacinum strain into a liquid seed culture medium for pre-culture; the method comprises the following steps: inoculating pre-cultured schizochytrium limacinum into a fermentation tank according to the inoculum size of 5-10% v / v, culturing for 48-60 hours at the sea salt concentration of 0.5-1% w / v, fermenting for 48-72 hours, supplementing sea salt to a culture medium in batches until the sea salt concentration is 3-5% w / v, and continuously culturing for 96-120 hours by controlling the glucose concentration and the yeast extract concentration through supplementing materials. The technical problem that the synthesis efficiency of schizochytrium limacinum phospholipid type EPA is low is solved through precise regulation and control of salinity stress, an efficient, economical and generalizable innovative scheme is provided for industrial production of functional lipid, and the method is suitable for large-scale production.

To provide a topical skin preparation that effectively promotes collagen production within the skin, exhibits excellent usability, and effectively enhances post-use stratum corneum moisture content.SOLUTION: A topical skin preparation comprises niacinamide, a heparin-like substance, dipotassium glycyrrhizinate, a collagen peptide, a yeast extract, and glycylglycine. It is preferable that the topical skin preparation further contains hydrogenated soybean phospholipids and phytosterols. It is more preferable that the topical skin preparation has a niacinamide content of 5 mass% or more. It is also preferable that the topical skin preparation further contains at least one plant extract selected from Arnica montana and Plantago major.SELECTED DRAWING: Figure 1

This invention provides a novel method for preparing a medical collagen sponge, relating to the field of medical sponge technology. The invention uses yeast extract obtained by Soxhlet extraction of brewer's yeast as a raw material, which is mixed and reacted with polyvinyl alcohol and graphene oxide to obtain the novel medical collagen sponge. The medical collagen sponge prepared by this invention exhibits excellent performance and good hemostatic effect. Furthermore, the preparation method is simple, and it degrades postoperatively, demonstrating significant application value in the field of medical materials.

The invention discloses a method for improving the fermentation performance of clostridium aerovorans and application of the clostridium aerovorans, and according to the method, an effective amount of methionine or a derivative thereof is added into a fermentation culture medium of the clostridium aerovorans to completely replace expensive complex organic nitrogen sources such as yeast extracts. The method successfully solves the three technical bottlenecks of slow biomass accumulation, low ethanol yield and dependence on expensive nitrogen sources in the fermentation process of the clostridium aerovorans by utilizing synthesis gas or CO2 / H2 mixed gas. Experiments prove that methionine or a derivative thereof can synergistically promote growth metabolism of thalli and a synthesis path of a product, so that the gas conversion efficiency of a carbon source is remarkably improved and metabolism is guided to flow to ethanol synthesis while the growth rate and the final biomass of the thalli are improved, and thus the production capacity of alcohols is synchronously enhanced. The method is simple in process and remarkable in effect, and an efficient and economical new strategy is provided for reducing the production cost of biofuel and promoting industrial application of a synthesis gas biorefinery technology.

The invention relates to the technical field of microbial fermentation, in particular to a fermentation medium for producing oxytetracycline. According to the fermentation culture medium, a corn starch-molasses-wheat hydrolyzed sugar composite carbon source, a soybean cake powder-corn steep liquor-yeast extract composite nitrogen source and a choline chloride-betaine-L-lysine precursor accelerant in a specific proportion are used as a core innovative system, and a form regulating agent, a defoaming agent and inorganic salt are cooperatively added. Through the synergistic effect of multiple components, the fermentation titer and yield of oxytetracycline are remarkably improved, the fermentation period is shortened, the generation of heteroacid is effectively inhibited, the mycelium form is optimized, and the oxytetracycline fermentation medium is suitable for industrial large-scale production of oxytetracycline.

The invention discloses a fermentation process for shortening a fermentation period and efficiently synthesizing Ectoine. The fermentation process comprises the following steps: screening and activating high-yield strains, preparing a fermentation culture medium, performing fermentation culture, performing sectional dissolved oxygen regulation and control, performing double-stage pH dynamic regulation and control, performing carbon-nitrogen ratio dynamic regulation based on metabolic flow analysis, performing osmotic pressure gradient induction in the later stage of fermentation, and extracting and purifying a product. According to the fermentation process for shortening the fermentation period and efficiently synthesizing the Ectoin, the expression quantity of key enzymes for synthesizing the Ectoin is increased by 80% or above compared with that of an original strain through the Halomonassp.ECT-09 strain bred through ultraviolet-nitrosoguanidine compound mutation, the basic yield reaches 35 g / L, a foundation is laid for high yield, and the method is suitable for industrial production. A glucose-trehalose mixed carbon source and a yeast extract-ammonium nitrate mixed nitrogen source are adopted, and an L-aspartic acid-L-asparagine mixed precursor is added, so that the metabolic pathway is shortened, and the loss of an intermediate product is reduced.

The invention relates to the technical field of skin care product raw materials, and particularly discloses a composition for controlling oil and removing acne and application thereof. The composition for controlling oil and removing acne comprises the following components in parts by mass: 3.2478-5.413 parts of a humectant; 3-5 parts of a penetration enhancer; 0.0072 to 0.012 part of a preservative; 3.1164 to 5.194 parts of a composite extract product; 2.6286 to 4.381 parts of a solvent; the composite extract is prepared by compounding a lotus corniculatus seed extract, a yeast extract, a rosa multiflora flower extract and a parsley flower extract. The acne-removing mask has the advantage of further improving the acne-removing effect.

The invention discloses a culture medium for identifying gardnerella vaginalis, and relates to the technical field of bacterial culture media, the key points of the technical scheme are as follows: the culture medium comprises the following raw material components by concentration: 10.0 g / L of casein pancreatin digest, 3.0 g / L of cardiac pancreatin digest, 5.0 g / L of yeast extract powder, 1.0 g / L of corn starch, 5.0 g / L of sodium chloride and 13.0 g / L of agar, and the final concentration of defiberized sheep blood is 5%. The pharmaceutical composition is prepared from 1 mg / mL of sodium alginate, 1 mg / mL of sodium alginate, 1 mg / mL of polyanethole Sulfonate (SPS) and 10 [mu] g / mL of vancomycin. According to the invention, through optimal combination of specific components and dosages thereof, rapid identification and screening of gardnerella vaginalis can be realized, and the method has the advantages of accurate result, strong specificity and the like, and has practical popularization and application values.

The invention belongs to the technical field of food processing, and particularly discloses a preparation process of natural preservative chicken soup based on lactobacillus fermentation. The process mainly comprises the following steps: decocting chicken and water according to a specific ratio to obtain basic chicken soup; the preparation method comprises the following steps: adding lactose, a yeast extract and the like as fermentation substrates, sterilizing, and inoculating a compound lactic acid bacteria starter consisting of lactobacillus plantarum and pediococcus pentosaceus to carry out anaerobic directional fermentation until the pH value is reduced to 4.0-4.6; and after fermentation is completed, adding natural preservative enhancers such as lactoferrin, and finally, homogenizing and filling to obtain the product. Natural preservation and flavor improvement of the chicken soup are synchronously achieved through a biological fermentation method, the obtained product is high in bacteriocin yield and remarkable in pathogenic bacterium inhibition effect, long-acting preservation can be achieved without adding chemical preservatives, meanwhile, the content of free amino acid and umami substances is greatly increased, and the fresh-keeping effect is good. The method is suitable for industrial production of high-quality concentrated chicken soup seasoning and liquid soup base.

The present application relates to a kind of skin care oil compositions for sensitive skin damage repair and its application, the skin care oil compositions include microbial oil and base oil, the microbial oil is with perilla seed powder, almond powder, camellia seed powder, glucose, protein peptone, yeast extract as substrate, using schizochytrium and lactobacillus plantarum to ferment substrate, the microbial oil obtained by fermentation has good effect of repairing skin damage.

The invention belongs to the technical field of hotpot condiments, and particularly relates to a bovine bone marrow oil mellow and hot hotpot condiment and a preparation method thereof. Comprising the following raw materials in parts by weight: 205 to 298 parts of edible beef tallow, 9.5 to 14 parts of edible bovine bone marrow oil, 15 to 25 parts of dried chili, 18 to 26 parts of chafing dish bean halves, 4 to 6 parts of fermented soya beans, 13 to 20 parts of monosodium glutamate, 10 to 16 parts of edible salt, 7 to 12 parts of fresh ginger, 9 to 14 parts of garlic, 7 to 11 parts of shallots, 10 to 16 parts of spices, 4.5 to 7 parts of yeast extract, 3 to 4.5 parts of white granulated sugar, 0.5 to 0.97 part of disodium 5 '-ribonucleotide, 15.8 to 23.8 parts of edible flavors and fragrances and 0.5 to 0.9 part of capsanthin. The edible bovine bone marrow oil in the mellow and hot hotpot condiment product containing the bovine bone marrow oil has rich and deep animal fat fragrance, the fragrance is mellower than beef tallow, the condiment is good in mellowness, and the flavor and taste are improved.

This invention discloses a processing technology for antibody-enhancing probiotic feed, which includes raw material pretreatment, substrate preparation, sterilization, cooling, strain activation, aseptic inoculation, constant-temperature anaerobic fermentation, immune-enhancing compounding, filtration and homogenization, and aseptic filling and storage. The fermentation substrate liquid includes purified water, molasses, yeast extract, compound trace elements, and buffer salts. A compound microbial community composed of *Lactobacillus plantarum*, *Bacillus subtilis*, *Bifidobacterium*, and yeast is used for fermentation. The temperature is controlled at 35–39°C during fermentation, and intermittent stirring is employed. After fermentation, astragalus polysaccharide, echinacea extract, and trehalose are added for compounding. This invention reduces the loss of active ingredients by adding immune-enhancing components after fermentation, and improves the survival rate of probiotics and fermentation stability through intermittent stirring. The resulting liquid feed has a high live bacteria content and immune-enhancing effect.

The invention discloses a fish hydrolyzed collagen peptide-based flavor beverage and a preparation method thereof, and relates to the technical field of food beverages. The flavored beverage comprises the following raw materials: fish hydrolyzed collagen peptide, a compound flavor modification stabilizer, a compound sweetening agent, concentrated fruit and vegetable juice, casein phosphopeptides, VC magnesium phosphate, zinc lactate, a low-nucleic-acid yeast extract, sodium hyaluronate, an acidity regulator and purified water. The compound flavor modification stabilizer is matched with the low nucleic acid yeast extract to form synergistic flavor regulation and control, the hydrophobic bitter peptide fragment of the fish hydrolyzed collagen peptide and the fishy smell characteristic group are wrapped from the molecular level, and meanwhile, the soft modification on the sensory level is realized through the gamma-aminobutyric acid; the effect of debitterizing and deodorizing without adding artificial essence and a large amount of cane sugar is achieved, the product flavor is natural and harmonious, the sour and sweet taste is palatable, the beverage completely conforms to the current healthy consumption trend of low sugar and no artificial addition, and the problem that the beverage has flavor defects is solved.

The preparation method comprises the following steps: carrying out fishy smell removal treatment on a beef offal raw material, and carrying out enzymolysis on the treated low-fishy-smell beef offal to obtain beef offal enzymatic hydrolysate; chopping the mixed fresh food, adding vegetable oil, heating and frying to obtain fresh food residues and fresh food oil; taking beef offal enzymatic hydrolysate, mixed spices, soy sauce, yeast extract, yolk powder, fresh seasoning oil, fresh residues, raw tallow, amino acid, reducing sugar, edible salt, white granulated sugar, monosodium glutamate and water; the preparation method comprises the following steps: uniformly mixing all the raw materials, carrying out thermal reaction, cooling, discharging, sieving to obtain reaction feed liquid, and concentrating the feed liquid until the water activity is less than or equal to 0.7. The beef offal flavor seasoning paste with natural and harmonious flavor is prepared by combining enzymolysis, Maillard reaction, low-temperature concentration and other technologies while the fishy smell removal efficiency is remarkably improved by adopting a triple fishy smell removal technology of enzymolysis, ultrasonic cleaning and fresh raw material synergistic treatment.

The application provides a sheep fattening complete granular feed and a preparation method and a feeding method thereof, relates to the technical field of feed, and the sheep fattening complete granular feed comprises the following components: ginseng stem and leaf, red yeast extract and sheep group basic feeding daily ration. The application solves the technical problems of high total ginsenoside extraction cost, low single stomach animal utilization rate, slow growth during the sheep fattening period and frequent outbreaks of diseases, achieves the technical effects of improving ginsenoside utilization efficiency, synergistically improving sheep immunity and improving the average daily weight gain of sheep during the whole period, and meanwhile, the process is simple, and the cost is low.

The invention relates to the technical field of microorganisms, and discloses efficient detoxification of a strain of pseudoxanthomonas to ochratoxin A and application of the strain of pseudoxanthomonas to the ochratoxin A. A strain of OTA efficient detoxification strain pseudoxanthomonas X-22 is screened out, and the pseudoxanthomonas X-22 can reduce 50 mu g / mL of OTA (10000 times of the national standard) to 0 within 70 min. The strain is mainly used for performing biological detoxification on OTA through intracellular enzyme, and a detoxification product is OTalpha with extremely low toxicity. The optimal growth conditions of the pseudoxanthomonas X-22 are as follows: 1.5 wt% of glucose is added into a basic culture medium as a carbon source, 1.5 wt% of yeast extract is added into the basic culture medium as a nitrogen source, the pH value of the culture medium is adjusted to 7.5, and the culture temperature is 37 DEG C. The OTA detoxification strain pseudoxanthomonas X-22 is applied to fermentation detoxification of polluted corn and oat, the detoxification rates of the corn and the oat are 61.29% and 97.26% respectively, OTA detoxification strain resources are enriched, and a theoretical basis is provided for application of a biological detoxification technology in the corn and the oat.

1. Name of the designed product: packing bag (yeast leaflet). 2. Use of the designed product: packing of nutritious fertilizer. 3. Design points of the designed product: in the pattern. 4. Picture or photo that best shows the design points: front view. 5. The designed product is a thin product, and other views are omitted.

This invention discloses a culture medium for the mass sporulation of *Substantia macrantha* and its application. The culture medium comprises the following components: lactose 21-23 g / L, yeast extract 14-16 g / L, potassium chloride 0.4-0.6 g / L, magnesium sulfate 0.4-0.6 g / L, potassium dihydrogen phosphate 0.4-0.6 g / L, disodium hydrogen phosphate 0.55-0.75 g / L, sodium molybdate dihydrate 5-7 mg / L, vitamin B6 50-60 mg / L, arginine 20-30 mg / L, and agar 18-22 g / L, diluted to volume with double-distilled water, with a natural pH. Using the culture medium prepared according to this invention to culture *Substantia macrantha* significantly increases the spore yield (164-fold), reaching 2.21 × 10⁻⁶. 8 conidia / cm 2 This provides a reference for the mass production of the fungal spores.

The application provides a roasted sesame salad dressing and a preparation method thereof, and relates to the field of condiments. The method comprises the following steps: taking egg yolk powder, white granulated sugar, vinegar, salt and mustard powder, adding water, and mixing uniformly to obtain a liquid 1; adding vegetable oil to the liquid 1, homogenizing to a paste, and obtaining a liquid 2; adding vinegar, monosodium glutamate, sesame fragments, soy sauce, lentinus edodes extract, yeast extract, white pepper powder, sucralose and water to the liquid 2, mixing uniformly, and obtaining a liquid 3; adding xanthan gum to the liquid 3, stirring, passing through a colloid mill, and obtaining a liquid 4; sterilizing the liquid 4 by heating while stirring, keeping warm, filling, and obtaining the roasted sesame salad dressing. The roasted sesame salad dressing prepared by using the optimized formula has the advantages of bright color, good and stable texture, rich taste, strong aroma, excellent sensory quality, and obviously reduced oil separation phenomenon when stored at room temperature, and the consumer experience is improved.

The invention discloses a composite microbial agent for degrading micro-plastics in seawater and a preparation method thereof, the microbial agent is composed of effective viable bacteria and auxiliary components, the effective viable bacteria are marine-derived halomonas, acinetobacter and bacillus and pseudomonas with high affinity with the micro-plastics, the effective viable bacteria are compounded according to a specific viable bacteria number ratio, and the microplastics are degraded by the auxiliary components. And function collaboration is realized. The auxiliary components comprise a protective agent consisting of trehalose, skimmed milk, glycerol and cane sugar, and a nutritional agent consisting of glucose and yeast extract. The preparation method comprises the following steps: respectively fermenting, centrifugally concentrating, compounding in proportion, mixing with auxiliary components, and finally drying to prepare powder. The invention further discloses application of the microbial inoculum to degradation of microplastics in beach intertidal zones and ship wastewater systems. The microbial agent is high in pertinence, good in stability in a high-salt seawater environment and high in degradation efficiency, and an effective solution is provided for in-situ bioremediation of marine micro-plastic pollution.

The application discloses a novel plant protein meat strip and a preparation method thereof, and belongs to the technical field of food processing. The plant protein meat strip is prepared from the following raw materials in parts by weight: soybean protein isolate 30-40 parts, pea protein 20-25 parts, gluten powder 10-15 parts, lentinus edodes powder 8-12 parts, konjac powder 5-8 parts, corn starch 10-15 parts, sunflower seed oil 5-8 parts, yeast extract 2-3 parts, carrot powder 2-4 parts, celery powder 1-2 parts, salt 0.8-1.2 parts, ginger powder 0.5-1 part and garlic powder 0.5-1 part. The preparation method comprises the following steps: mixing ingredients, multi-section temperature control double-screw extrusion molding, low-temperature marinating using specific plant-based broth, drying and high-temperature sterilization. Through protein compounding and process optimization, the application solves the problems of poor texture and single flavor of the existing plant meat strips, the product has a taste close to real meat, the flavor is natural and mellow, and the product is suitable for industrial production.

The application provides a yeast mannose oligosaccharide and a preparation method and application thereof. The preparation method comprises the following steps: S1, mixing yeast cell walls with water to obtain a mixed solution; S2, adjusting the pH of the mixed solution to 4-9, performing first enzymolysis on the mixed solution by using a protease to form a first enzymolysis product; secondly, performing second enzymolysis on the first enzymolysis product by using a polysaccharide enzyme to obtain a second enzymolysis product; the amount of the protease is greater than or equal to 0.2% of the weight of the yeast cell walls, and the amount of the polysaccharide enzyme is greater than or equal to 0.1% of the weight of the yeast cell walls; S3, centrifuging the second enzymolysis product to obtain a supernatant and a centrifugal heavy phase; filtering the supernatant by using an ultrafiltration membrane, and the retentate is the yeast mannose oligosaccharide, the permeate is a yeast extract, and the centrifugal heavy phase is a yeast glucan. The yeast mannose oligosaccharide obtained by the application has high purity and high yield, the mannose oligosaccharide is clean, can be fermented by beneficial bacteria, and can be applied as a prebiotic in food.

The invention discloses an amino acid facial cleanser composition and application thereof, and aims to overcome the defects that the existing acne-removing facial cleanser is high in irritation, acne removal and mild cleaning are difficult to consider, and a single component only acts on a single target spot of acne. According to the composition, 5.0-25.0 wt% of sodium cocoyl glycinate, 0.1-2.0 wt% of capryloyl glycine, 0.2-5.0 wt% of nicotinamide and 0.01-0.5 wt% of a hydrolysis yeast extract are taken as core active components, the four components are specifically proportioned to form a synergistic effect, oil control and acne removal are realized through a closed-loop passage of pore cleaning, source oil control and targeted bacteriostasis, and the mild property is guaranteed by amino acid surface activity, and the skin barrier is not damaged. The composition disclosed by the invention can be prepared into facial cleanser and facial mousse, and is excellent in cleaning power and foamability, good in use experience and high in industrialization value.

The invention relates to an eel fry feed containing nucleic acids. It is preferable that the content of the nucleic acids is 0.25 wt% or more based on the total dry matter weight of the feed for the eel fry. The feed for the eel fry comprises a yeast extract, and the yeast extract can contain the nucleic acid.

The invention provides a method for efficiently extracting ursolic acid from loquat leaves and application of the ursolic acid. Comprising the following steps: S1, taking fresh folium eriobotryae, cleaning, and drying at 45 DEG C until the water content is 1t; after 8%, crushing, sieving with a 60-mesh sieve, and sterilizing with microwaves to obtain sterilized loquat leaf powder; s2, mixing sterilized folium eriobotryae powder, a yeast extract, MgSO4. 7H2O and water, adjusting the pH value to 5.0, taking the mixture as a fermentation culture medium, synchronously inoculating a 5% saccharomyces cerevisiae seed solution and a 10% trametes versicolor seed solution, and performing shake culture at the temperature of 30 DEG C and the speed of 150 rpm for 36-48 hours; s3, adding a surfactant, uniformly stirring, and performing ultrasonic extraction to obtain an extracting solution; and S4, centrifuging the extracting solution at 4000-5000 rpm for 10-15 minutes, taking supernate, filtering with a filtering membrane, concentrating and freeze-drying. According to the method, double-bacterium synchronous synergistic fermentation and a high-dose surfactant-assisted ultrasonic extraction technology are combined, so that a strong synergistic effect is formed, and the problems of low extraction rate, poor purity, high energy consumption, large solvent dosage and the like in a traditional loquat leaf ursolic acid extraction method are fundamentally solved.

The purpose of the present invention is to provide a food-or-beverage product composition that contains a yeast material (in particular, a residue during the production of a yeast extract). The food-or-beverage product composition: contains the yeast material exclusively as the main raw material; is easy to produce; and is capable of imparting a cacao or cocoa flavor to food-or-beverage products, is capable of enhancing the cacao or cocoa flavor of food-or-beverage products, or is capable of being used as a substitute material for cocoa mass.　The present invention provides: a food-or-beverage product composition that contains a yeast material, that has a dietary fiber content of at least 35 mass% and a glutamic acid content of less than 1 mass%, and that is characterized in that the yeast material contains 4-hydroxy-2,5-dimethyl-3(2H)-furanone at an internal standard ratio of at least 2.0 when 5-nonanone is used as an internal standard; and a method for producing the food-or-beverage product composition.

To provide a masking agent for off-flavors and off-odors derived from soy protein that can reduce off-flavors and off-odors derived from soy protein, a method for masking off-flavors and off-odors derived from soy protein, and a method for producing soy protein processed foods. [Solution] A masking agent for off-flavors and off-odors derived from soy protein, which contains yeast extract and fermented flavor liquid.

The invention relates to a propionibacterium acnes selective culture medium and a preparation method thereof, the culture medium comprises casein pancreatin digest, meat and stomach enzyme digest, heart and pancreatin digest, yeast extract powder, corn starch, sodium chloride, agar and defiberized sheep blood, and the volume is fixed with distilled water. Compared with an existing culture medium, the culture medium has the advantages of being reliable in detection and high in propionibacterium acnes separation rate and has a good application prospect.