39 results about "Respiratory quotient" patented technology

A control system for a controlled atmosphere room (“CA room”) for storing perishable commodities, such as fruits and vegetables. The control system includes an enclosure that can be placed within the CA room to store a representative sample of the commodities in the CA room. The control system includes an atmosphere valve selectively operable to provide atmospheric communication between the enclosure and the CA room or to isolate the enclosure from the CA room. The control system includes a sampling control system for determining a dynamic control value based on the isolated representative sample. The dynamic control value may be determined by monitoring the respiratory quotient in the enclosure while it is isolated. Once determined, the control system can use the dynamic control value to adjust the atmosphere of the CA room, thereby using tests on a representative sample to control the atmosphere for the full volume of commodities in the CA room. When not testing, the enclosure generally remains in atmospheric communication with the CA room, which improves the correlation of the representative sample with the commodities in the CA room.

A method and apparatus for determining a user's Respiratory Quotient (RQ) using just measured O2 and CO2 concentrations without use of a flow meter. The RQ is determined by measuring the user's real-time inspired O2 concentration (INS O2) and end tidal O2 concentration (ETO2) and measuring the user's real-time inspired CO2 concentration (INS CO2) and end tidal CO2 concentration (ETCO2), and then determining the user's RQ from the measured INS O2, ETO2, INS CO2, and ETCO2 values in accordance with the following equation: RQ=(ETCO2−INS CO2) / (INS O2−ETO2). In order to avoid error introduced by the flow rate, the measurement steps are preferably performed while the user is in a resting condition. Also, ETCO2 is preferably measured as the maximum CO2 value in a breath cycle of the user, while INS CO2 is preferably measured as the minimum CO2 value in a breath cycle of the user. Similarly, ETO2 is preferably measured as the minimum O2 value within a breath cycle of the user, while INS O2 is measured as the maximum O2 value within a breath cycle of the user. On the other hand, the values of INS CO2 and ETCO2 also may be determined in accordance with the invention by analysis of a CO2 waveform of a breath cycle of the user and the values of INS O2 and ETO2 determined by synchronizing timing of the O2 waveform of a breath cycle of the user with the CO2 waveform and sampling INS O2 and ETO2 values simultaneously with sampling of complementary CO2 values determined by analysis of the CO2 wavefomm. The RQ measuring device may include the oxygen and CO2 sensors in a mainstream or sidestream configuration.

The invention provides a method for process optimization and data amplification for bioreactors. The method comprises the following steps: (a) measuring the physiological metabolism parameter, and physiologic metabolism parameter correlation related characteristics or combination thereof of each bioreactor, wherein the physiologic metabolism parameter is selected from oxygen dissolution rate, oxygen intake rate, respiratory quotient, volume oxygen transfer coefficient or combination thereof; (b) comparing the physiologic metabolism parameter and physiologic metabolism parameter related characteristics measured in step (a) with the preset physiologic metabolism parameter, and physiologic metabolism parameter related characteristics or combination thereof; selecting the bioreactor of which the physiologic metabolism parameter and physiologic metabolism parameter related characteristics most approaching to the preset values; and determining the optimized amplification bioreactor, wherein the physiologic metabolism parameter is specified in step (a).

The invention relates to a method for automatically calibrating the tidal volume of an anesthetic machine, comprising the following steps that: firstly, the respiratory frequency and the respiratroy quotient of the anesthetic machine are set; secondly, an initial point and a plurality of intermediate flow rate points are selected as index points within a calibrated flow rate range; thirdly, the value of the tidal volume is obtained through calculation of a formula V=FT; fourthly, a calibrated tidal volume measuring device is used as reference to search for calibrated tidal volumes one by one and automatically save PWM values of searched tidal volumes; fifthly, a fitting curve chart of the flow rate L and the PWM values is established by utilization of the calibrated tidal volumes according to the set respiratory frequency, the set respiratroy quotient and the corresponding flow rate calculated in the formula of the third step; the flow rate value can be calculated according to the set tidal volume under the condition of the set respiratory frequency and the set respiratroy quotient; and the PWM value at the time can be calculated according to the fitting curve chart to control the flow rate, thereby the method realizes automatic calibration of the tidal volume and avoids affection of the dead space volume of devices, the aperture of pipes, the self weight of windboxes and the external factors on calibration of the tidal volume.

The invention pertains to the establishment, implementation and management of a personalized information system pertinent to a user's general health, wellness and / or sport performance. A novel set of portable devices with calorimetric, metabolic sensing, computing and communication capabilities are used for non-stop real-time measurement and display as well as long-term logging of highly accurate information about a user's metabolic state. Continuous real-time feedback on the user's respiratory quotient (RQ) and other data may be provided. A novel dual-battery system is also provided by which an uninterrupted power supply can be provided for electronic components. The personalized information system is further designed to consider measured and calculated metabolic parameters in relation to manually specified, user-specific goal(s), and to provide feedback about the user's progress with regards to these goals over the short- and long term. The system may be used to continuously determine the real-time nutritional state, energy uptake and energy expenditure level of the user, and may provide subsequent personalized nutritional- and exercise guidance to advance the user's efficiency at achieving and maintaining his / her specific health, wellness and / or sport performance goal(s). The invention also integrates user information, such as, but not limited to real-time energy uptake and real-time energy balance with social networking / gaming and other social interactions.

The invention discloses a control method for gluconate production by a microbiological method. A high-yield gluconic acid strain is adopted as an original strain, which is moved into a fermentation tank for fermentation production of gluconate after seed culture. During fermentation, according to an on-line respiratory quotient (RQ), the adding speed and adding amount of glucose can be controlledin real time. The control method of the invention can make the glucose consumption rate reach 12g / L / h, and the conversion rate of production in the fermentation tank reach over 110%, thus realizing automatic flow feeding and substantially reducing the production cost.

A citric acid preparation method comprises a step that aspergillus niger is inoculated to fermentation liquor to produce citric acid in a fermentation way, wherein the fermentation temperature of the rise phase of the aspergillus niger respiratory quotient is 35-45 DEG C, the fermentation temperature of the decline phase of the aspergillus niger respiratory quotient is 30-40 DEG C, the fermentation temperature of the low-level maintenance phase of the aspergillus niger respiratory quotient is 30-40 DEG C, and the fermentation temperature of the rebound phase of the aspergillus niger respiratory quotient is 35-45 DEG C; the fermentation temperature of the rise phase of the aspergillus niger respiratory quotient is higher than that of the decline phase of the aspergillus niger respiratory quotient, the fermentation temperature of the decline phase of the aspergillus niger respiratory quotient is higher than that of the low-level maintenance phase of the aspergillus niger quotient, the fermentation temperature of the rebound phase of the aspergillus niger respiratory quotient is higher than those of the decline phase of the aspergillus niger respiratory quotient and the low-level maintenance phase of the aspergillus niger respiratory quotient. With the adoption of the method, the fermentation efficiency can be effectively improved, and the fermentation period is shortened.

The invention discloses a genetically engineered bacterium for directly producing L-aspartic acid through fermentation. The classification naming of the genetically engineered bacterium is Escherichia coli CM-AS-115, and the preservation number of the genetically engineered bacterium is CCTCC NO: M 2016457. The bacterial strain relates to inactivation of multiple genes, evolution, metabolism and domestication are simultaneously carried out on the bacterial strain which knockouts the multiple genes, and a mutant strain, namely, CM-AS-105, which has a lower respiratory quotient under the aerobic condition and of which the highest dry cell weight is 60-70% of dry weight of an original strain W1485 is obtained; meanwhile, the bacterial strain further relates to over expressions of two genes, wherein the two genes comprise an enol phosphate type pyruvate carboxylase encoding gene (ppc) and an aspartase encoding gene (aspA), and the obtained bacterial strain is CM-AS-115. The genetically engineered bacterium for directly producing L-aspartic acid through fermentation achieves a way of completely adopting renewable biomass resources such as starch and cellulose as raw materials to ferment and prepare the L-aspartic acid, and the way is green and environmentally friendly.

A system for determining resting metabolic rate (RMR) based primarily on oxygen consumption is described. The system consists of an enclosed, sealed chamber within which the patient / subject sits or reclines. The rate of volumetric oxygen change in the system is then monitored to determine the subjects' oxygen consumption rate. The rate of the subjects volumetric oxygen consumption is then converted by the system, using the widely accepted Weir formulation, to calculate RMR. The system is capable of making this calculation in under 10 minutes, making the measurement highly convenient for the subject. The system contains a display mounted on or within the chamber to both serve as a user interface, as well as to serve as a platform for informative programming such as advertisements for weight loss and diet centers and nutrition related products. The system contains a money dispensing system in order that users can pay for the metabolic test. Finally, the system is also capable of obtaining two ancillary parameters, percent body fat and respiratory quotient.

The invention provides a method for optimizing and scaling up the fermentation process using a bioreactor device. The method comprises the following steps: 1. measuring physiological parameters and the related characteristics or combined characteristics of the physiological parameters of the fermentation process of the bioreactor device, wherein the physiological parameters are selected from the following parameters: dissolved oxygen, oxygen uptake rate, pH, carbon dioxide excretion rate, respiratory quotient, living cell rate or cell shape, consumption of measured metabolic product or matrix or combined characteristics; and 2. comparing the physiological parameters and the related characteristics or combined characteristics which are measured in the step 2, with preset physiological parameters and the related characteristics or combined characteristics; selecting a bioreactor device of which data are most similar to the preset values; and determining the optimized and scaled-up bioreactor device, wherein the physiological parameters are shown in the step 1.

The invention provides a method for process optimization and data amplification for bioreactors. The method comprises the following steps: (a) measuring the physiological metabolism parameter, and physiologic metabolism parameter correlation related characteristics or combination thereof of each bioreactor, wherein the physiologic metabolism parameter is selected from oxygen dissolution rate, oxygen intake rate, respiratory quotient, volume oxygen transfer coefficient or combination thereof; (b) comparing the physiologic metabolism parameter and physiologic metabolism parameter related characteristics measured in step (a) with the preset physiologic metabolism parameter, and physiologic metabolism parameter related characteristics or combination thereof; selecting the bioreactor of which the physiologic metabolism parameter and physiologic metabolism parameter related characteristics most approaching to the preset values; and determining the optimized amplification bioreactor, wherein the physiologic metabolism parameter is specified in step (a).

A method and system are provided for simulating the metabolic consumption of oxygen contained in a breathable gas. A variable volume chamber cyclically increases / decreases in volume to receive the breathable gas / expel an exhaust gas. Hydrogen and carbon dioxide are introduced into the chamber to mix with the breathable gas to form the exhaust gas. Hydrogen is introduced in an amount sufficient to react with an amount of the oxygen in the exhaust gas equivalent to that used by a human during a selected level of activity. Carbon dioxide is introduced in an amount equivalent to that provided by a metabolic respiratory quotient associated with the same level of activity. A catalyst, exposed to the exhaust gas, causes a reaction between the hydrogen and oxygen in the exhaust gas to generate simulated human exhalation.

A method for the determining the ratio of oxygen consumption to carbon dioxide production, commonly known as the respiratory quotient, in expired breath, and a device to carry out the method without providing volumetric measurements.

The invention relates to a fermentation method and particularly relates to a method for preparing 1,5-pentanediamine by fermentation. The method for preparing 1,5-pentanediamine by fermentation comprises the steps of converting a substrate into 1,5-pentanediamine through bacterial fermentation and controlling RQ (respiratory quotient) to be 1.2-2.2 by controlling a speed for adding ammonium sulfate in stages in the fermenting process. The method can solve the technical problem of low pentanediamine conversion rate in a current preparation method.

The invention relates to a method for fermentative production, on an industrial scale, of lipid-rich biomass of microalgae of the Chlorella genus having acceptable sensory properties, characterized in that the dissolved oxygen availability in the fermenter is controlled by tracking the respiratory quotient of said microalgae.

The invention discloses a soft and elastic coat. When the elastic coat is not worn with the naturally tensionless state, the part, more than 70% of body girth, consists of elastic weft-knitting fabric; when the elastic weft-knitting fabric is in the 30-80% extension range, the tight index is below 0.8, and the restoring stress is 0.3-1.3N. When the elastic coat is worn for exercise with the exercise intensity of being below 1Mets, the exercise time of being below 6 hours and the amount of body exercise of being below 25Mets. hr, the respiratory quotient is below 1.05. When the elastic coat is worn with the state of no action, the clothes pressure is below 0.6kPa, and when the elastic coat is worn with the state of motion, the clothes pressure is below 2.5kPa.

The invention discloses a basal metabolism measuring device. The basal metabolism measuring device comprises a housing (1), a control device (2), an oxygen concentration measuring device (3), a carbondioxide concentration measuring device (4), a flow measuring device (5) and a collecting pipeline (6), wherein the oxygen concentration measuring device (3), the carbon dioxide concentration measuring device (4), the flow measuring device (5) and the collecting pipeline (6) are connected with the control device (2); the flow measuring device (5) is arranged at an air inlet of the measuring deviceand connected with the collecting pipeline (6); the air outlet end of the collecting pipeline (6) is connected with the air inlet end of the oxygen concentration measuring device (3); the air outletend of the oxygen concentration measuring device (3) is connected with the air inlet end of the carbon dioxide concentration measuring device (4); and the air outlet end of the carbon dioxide concentration measuring device (4) is connected with the backflow end of the collecting pipeline. The flow speed, the carbon dioxide concentration and the oxygen concentration of exhaled air of a human body can be measured in real time, and the respiratory quotient and the metabolic rate can be calculated according to the weight and the height of the human body. The basal metabolism measuring device is small, exquisite, quite convenient to carry and easy to operate.

Apparatus and methods for assessing metabolic substrate utilization are described. In one embodiment, a processor-readable medium includes code to receive data indicative of a plasma glucose concentration, a plasma free fatty acid concentration, and a respiratory quotient of a subject. The processor-readable medium also includes code to calculate, based on the data, respective values of a set of metabolic parameters. The set of metabolic parameters includes a first metabolic parameter and a second metabolic parameter. The value of the first metabolic parameter is indicative of whether the subject has a predisposition towards oxidation of a first type of metabolic substrate or a second type of metabolic substrate, and the value of the second metabolic parameter is indicative of the subject's responsiveness to a change in availability of the first type of metabolic substrate or the second type of metabolic substrate.

The invention relates to a method for fermentative production, on an industrial scale, of lipid-rich biomass of microalgae of the Chlorella genus having acceptable sensory properties, characterised in that the dissolved oxygen availability in the fermenter is controlled by tracking the respiratory quotient of said microalgae.

The invention relates to a method for fermentative production, on an industrial scale, of lipid-rich biomass of microalgae of the Chlorella genus having acceptable sensory properties, characterized in that the dissolved oxygen availability in the fermenter is controlled by tracking the respiratory quotient of said microalgae.

The present invention describes a method for producing an antibody in Pichia pastoris, such as by fed-batch fermentation. The method may include a strategy of increasing the ethanol concentration to 18-22 g / L and then maintaining the ethanol level at 5-17 g / L to stabilize the cell mass and enhance the production rate of the antibody. The method may also include the addition of 2.0-5.0 g / L of hydroxyurea during the fermentation process to sustain a constant cell density and enhance the whole broth titer of the antibody. The method may further include a respiratory quotient control for monitoring the ethanol profile and to improve the quality of the antibody by, for example, eliminating clipping of the heavy chain.

The invention discloses a potassium superoxide medical plate for an underground emergency refuge air regeneration device in a coal mine. The potassium superoxide medical plate comprises the following raw materials in part by weight: 94 to 96 parts of oxygenating and carbon absorbing medical powder and 4 to 6 parts of reinforcing agents; and the oxygenating and carbon absorbing medical powder comprises 75 to 85 weight percent of KO2, 8 to 13 weight percent of K2O2 and 7 to 12 weight percent of K2O. According to the potassium superoxide medical plate, the content of all active ingredients is appropriate, so that the oxygen releasing rate is reduced, the oxygen is stably and reliably generated, and the ratio of the absorbed carbon dioxide to the released oxygen is matched with the respiratory quotient of a human body; by adding auxiliary adding agents, the porosity of the medical plate is increased under the condition that the structural strength is not changed, so that the carbon dioxide penetration capacity is improved, and absorption of the medical plate to the carbon dioxide is promoted; and the humidity application range of the medical plate is expanded to over 90 percent, but the humidity application range of the conventional potassium superoxide medical plate is not more than 60 percent. The potassium superoxide medical plate is suitable for closed large spaces, high-temperature and high-humidity environments and crowded places.

A control system for a controlled atmosphere room (“CA room”) for storing perishable commodities, such as fruits and vegetables. The control system includes an enclosure that can be placed within the CA room to store a representative sample of the commodities in the CA room. The control system includes an atmosphere valve selectively operable to provide atmospheric communication between the enclosure and the CA room or to isolate the enclosure from the CA room. The control system includes a sampling control system for determining a dynamic control value based on the isolated representative sample. The dynamic control value may be determined by monitoring the respiratory quotient in the enclosure while it is isolated. Once determined, the control system can use the dynamic control value to adjust the atmosphere of the CA room, thereby using tests on a representative sample to control the atmosphere for the full volume of commodities in the CA room. When not testing, the enclosure generally remains in atmospheric communication with the CA room, which improves the correlation of the representative sample with the commodities in the CA room.

The invention discloses application of atractylenolide II for preparing a medicine capable of improving insulin resistance and glucose-lipid metabolism disorder caused by obesity. After mice are fed in a high fat mode, the weights of the mice are increased, and respiratory quotient and energy consumption are obviously reduced, and thus, fat mice mainly take lipid metabolism as a main part insteadof glucose metabolism as the main part, fat tissues are pathologically changed and lipid droplets are increased, and meanwhile, some index detection of blood plasma and liver also proves diseases of liver damage and insulin resistance of the mice. Based on the application of the atractylenolide II disclosed by the invention, after the atractylenolide II is provided for treatment, the weights and the glucose-lipid metabolism of the fat mice are improved, the lipid droplets of the livers are reduced, and the insulin resistance is recovered. Therefore, because of the atractylenolide II, the insulin resistance and the glucose-lipid metabolism disorder caused by the obesity can be effectively improved.

The invention provides a respiration work station and an oxygen supply method thereof. The respiration work station has a normal-frequency jet mode and a normal frequency / electrocardio trigger compound jet mode. A monitoring system receives human body electrocardiogram original data, analyzes the electrocardiogram original data, extracts a cardiac cycle and single / double frequency jet mode transition point data and sends the cardiac cycle and single / double frequency jet mode transition point data to a double-channel jet ventilation system. The double-channel jet ventilation system receives the cardiac cycle and single / double frequency jet mode transition point data sent by the monitoring station, combines the cardiac cycle and single / double frequency jet mode transition point data with aninput respiratory quotient to determine a double / single frequency jet mode transition point, implements transition from the normal frequency jet mode to the normal frequency / electrocardio trigger compound jet mode at the single / double frequency jet mode transition point and implements transition from normal frequency / electrocardio trigger compound jet mode to the normal frequency jet mode at the double / single frequency jet mode transition point. By the adoption of the respiration work station and the oxygen supply method thereof, the jet mode is adjusted according to the physiological stateof a user, and the purpose of improving the survival rate of patients is achieved.

The present invention describes a method for producing an antibody in Pichia pastoris, such as by fed-batch fermentation. The method includes the addition of about 2.0-5.0 g / L of hydroxyurea during the fermentation process to sustain a constant cell density and enhance the whole broth titer of the antibody. The method may also include a strategy of increasing the ethanol concentration to about 18-22 g / L and then maintaining the ethanol level at about 5-17 g / L to stabilize the cell mass and enhance the production rate of the antibody. The method may further include a respiratory quotient control for monitoring the ethanol profile and to improve the quality of the antibody by, for example, eliminating clipping of the heavy chain.

A citric acid preparation method comprises a step that aspergillus niger is inoculated to fermentation liquor to produce citric acid in a fermentation way, wherein the fermentation temperature of the rise phase of the aspergillus niger respiratory quotient is 35-45 DEG C, the fermentation temperature of the decline phase of the aspergillus niger respiratory quotient is 30-40 DEG C, the fermentation temperature of the low-level maintenance phase of the aspergillus niger respiratory quotient is 30-40 DEG C, and the fermentation temperature of the rebound phase of the aspergillus niger respiratory quotient is 35-45 DEG C; the fermentation temperature of the rise phase of the aspergillus niger respiratory quotient is higher than that of the decline phase of the aspergillus niger respiratory quotient, the fermentation temperature of the decline phase of the aspergillus niger respiratory quotient is higher than that of the low-level maintenance phase of the aspergillus niger quotient, the fermentation temperature of the rebound phase of the aspergillus niger respiratory quotient is higher than those of the decline phase of the aspergillus niger respiratory quotient and the low-level maintenance phase of the aspergillus niger respiratory quotient. With the adoption of the method, the fermentation efficiency can be effectively improved, and the fermentation period is shortened.

The invention provides a culture method of Aspergillus niger for producing citric acid. The method comprises the following steps: inoculating Aspergillus niger in an Aspergillus niger culture solution for culture, wherein the respiratory quotient of the Aspergillus niger thallus is 0.7-1.5 under the conditions of culture; and maintaining for 2-8 hours at the respiratory quotient. According to themethod provided by the invention, whether the growth state of the Aspergillus niger is applicable to subsequent fermentation is judged according to the numerical value of the respiratory quotient, and culture is maintained for 2-8 hours at the state that the respiratory quotient is 0.7-1.5, so that the obtained Aspergillus niger has high fermentation efficiency in the subsequent fermentation process; and the method provided by the invention is slightly influenced by personal factors, thereby maintaining the stability of Aspergillus niger culture among batches.

The invention discloses a process for producing salinomycin based on metabolizing parameter respiratory quotient (RQ) carbohydrate supplementation fermentation, which comprises the following steps of conducting fermentation cultivation; and inoculating seed liquid obtained by slant culture and seed culture to a fermentation tank, enabling the inoculum size to be 8-12%, enabling culture temperature to be 30-35 DEG C, enabling pressure to be 0.04-0.06 MPa, enabling DO to be controlled in 30%-100%, enabling rotation speed to be 250-500 rpm, beginning to adding carbohydrate supplementation when the RQ drops to a state lower than or equal to 0.65, enabling the RQ value to be controlled in 0.68-0.95, and placing a tank after 290-310 hours after fermentation begins. The process conducts flow-adding-type carbohydrate supplementation with the RQ value serving as operation indexes in the middle stage and later stage, reduces production cost of the salinomycin while stably improving titer, solves the technical problem that dissolved oxygen of a fermentation system is reduced caused by accumulation of excessive oil in a fermentation substrate, solves the technical problem of agglomeration of fermentation broth, and is mature in technique and suitable to industrial mass production.