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Citric acid preparation method

A citric acid and seed technology, applied in the direction of microorganism-based methods, biochemical equipment and methods, microorganisms, etc., can solve the problems of low efficiency of citric acid fermentation

Active Publication Date: 2012-03-14
COFCO BIOTECHNOLOGY CO LTD +1
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

[0004] The object of the present invention is to overcome the low defect of the citric acid fermentation efficiency of the prior art, and provide a kind of preparation method of the citric acid that can effectively improve the citric acid fermentation efficiency

Method used

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  • Citric acid preparation method

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preparation example Construction

[0042] According to the present invention, the preparation method of the Aspergillus niger culture solution is not particularly limited, as long as the obtained culture solution can be suitable for the cultivation of Aspergillus niger, for example, the preparation method of the Aspergillus niger culture solution may include: Add amylase, heat up to 90-100°C at a rate of 20-150°C / hour and spray once at this temperature, flash after 5-30 minutes, and wait until the temperature drops to 80-95°C at this Enzymatically hydrolyzing the starch at high temperature for 90-140 minutes to obtain a liquefied liquid and diluting the total sugar to 5-20% by weight, then adding nitrogen source and sterilizing. The term total sugar refers to the total content of sugar contained in the enzymatic hydrolysis liquefaction liquid.

[0043] According to the present invention, in the starch slurry, the contents of starch and water and the pH of the slurry can be changed in a wide range, preferably, t...

Embodiment 1

[0053] This embodiment is used to illustrate the preparation method of citric acid provided by the invention.

[0054] (1) 100 parts by weight of harvested corn (moisture content: 14% by weight) was pulverized to obtain a pulverized product having an average particle diameter of 400 μm.

[0055] (2) Mix the pulverized product with water at a concentration of 25% by weight and then adjust the pulp, and add 20 enzyme activity units of amylase (Novozymes, a-amylase) relative to the dry weight of every gram of the pulverized product ), enter the ejector, and perform enzymolysis at 85°C and pH 5.5 for 100 minutes to obtain enzymolysis product A1. Among them, 80% of the A1 enzymatic hydrolysis product is filtered by a hydraulic plate and frame filter press to separate the enzymatic hydrolysis clear liquid and the enzymatic hydrolysis solid phase residue.

[0056] (3) Use the enzymolysis product obtained in step (2) and the separated enzymolysis clear liquid to configure the ferment...

Embodiment 2

[0060] This embodiment is used to illustrate the preparation method of citric acid provided by the invention.

[0061] Citric acid was prepared according to the method of Example 1, except that: the enzymolysis product A1 obtained in Example 1 was used to configure the fermentation broth, and the specific composition was 85 parts by weight of enzymolysis liquefaction clear liquid and 14.8 parts by weight of enzymolysis solid phase The residue (solid content: 30% by weight) and 0.2 parts by weight of urea were sterilized and added to the fermenter to obtain fermented liquid B2.

[0062] The enzymolysis liquefaction clear liquid obtained in Example 1 is diluted with water to 10% by weight of total sugar and put into the seed tank, and urea is added. The amount of urea added is 0.35% of the total weight of the seed tank culture solution, heated to 120°C for disinfection, and maintained After 20 minutes, the temperature was rapidly lowered to 36°C, and the Aspergillus niger strain...

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Abstract

A citric acid preparation method comprises a step that aspergillus niger is inoculated to fermentation liquor to produce citric acid in a fermentation way, wherein the fermentation temperature of the rise phase of the aspergillus niger respiratory quotient is 35-45 DEG C, the fermentation temperature of the decline phase of the aspergillus niger respiratory quotient is 30-40 DEG C, the fermentation temperature of the low-level maintenance phase of the aspergillus niger respiratory quotient is 30-40 DEG C, and the fermentation temperature of the rebound phase of the aspergillus niger respiratory quotient is 35-45 DEG C; the fermentation temperature of the rise phase of the aspergillus niger respiratory quotient is higher than that of the decline phase of the aspergillus niger respiratory quotient, the fermentation temperature of the decline phase of the aspergillus niger respiratory quotient is higher than that of the low-level maintenance phase of the aspergillus niger quotient, the fermentation temperature of the rebound phase of the aspergillus niger respiratory quotient is higher than those of the decline phase of the aspergillus niger respiratory quotient and the low-level maintenance phase of the aspergillus niger respiratory quotient. With the adoption of the method, the fermentation efficiency can be effectively improved, and the fermentation period is shortened.

Description

technical field [0001] The invention relates to a preparation method of citric acid. Background technique [0002] Citric acid is an organic acid widely used in industries such as beverage, food and medicine. my country is a major producer of citric acid, with more than 20 production plants, and the output has exceeded 800,000 tons. [0003] At present, in the fermentation process of producing citric acid, when the seed liquid of Aspergillus niger is cultivated and matured and moved into the fermenter, the same control conditions are usually adopted throughout the fermentation process, resulting in the inability of Aspergillus niger to exert its optimal effect. resulting in lower fermentation efficiency. Therefore, there is an urgent need to develop a method that can make Aspergillus niger exert its maximum effect to improve the efficiency of citric acid fermentation. Contents of the invention [0004] The purpose of the present invention is to overcome the defect of low...

Claims

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Application Information

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Patent Type & Authority Applications(China)
IPC IPC(8): C12P7/48C12R1/685
Inventor 周永生张嗣良周勇冯志菲杨儒文
Owner COFCO BIOTECHNOLOGY CO LTD
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