5019 results about "Fermentation broth" patented technology

The invention relates to a fermentation process abnormal state early warning method, system and device and a medium, and the early warning method comprises the steps: collecting fermentation process parameters in a fermentation tank in real time, and generating a physical parameter matrix; obtaining Raman spectrum data of the fermentation liquor and extracting metabolic feature vectors; fusing the physical parameter matrix with the metabolic feature vector to obtain a multi-modal data cube; constructing a metabolic network model, inputting the physical parameter matrix as a boundary condition, and generating calibrated metabolic flux distribution data; dynamically adjusting the monitoring weight coefficient of the fermentation process parameter, and generating a parameter sensitivity weight vector; inputting the multi-modal data cube and the parameter sensitivity weight vector into a space-time convolutional network, extracting time sequence features and spatial distribution features, and performing similarity matching with a preset abnormal mode library to obtain a matching result; and determining an abnormity early warning type according to a matching result with abnormity, and generating a corresponding compensation control instruction. The stability of the fermentation process is improved.

The invention belongs to the technical field of bioengineering and the technical field of food engineering, and discloses a regulation and control method for improving the DHA content and the sn-2 DHA proportion in schizochytrium limacinum grease fermentation liquor, schizochytrium limacinum algae powder and application of the schizochytrium limacinum algae powder. In the fermentation culture process, the pH of fermentation liquor in the fermentation process is controlled by supplementing acid or alkali in stages. According to the method disclosed by the invention, the pH value of the fermentation liquor is regulated and controlled in stages in the fermentation culture process of the schizochytrium limacinum, and the schizochytrium limacinum can obtain a higher splitting propagation speed, a longer grease accumulation period and a shorter grease consumption end period by regulating and controlling the pH value in the fermentation process. According to the obtained schizochytrium limacinum grease, the oil yield is obviously improved compared with that of a traditional fermentation strategy, and the DHA content and the sn-2 DHA proportion in the oil are also obviously improved.

The present invention relates to the technical field of water-based coatings, and specifically to a PHA water-based coating, a preparation method and application thereof. The PHA water-based coating comprises the following raw materials: a PHA water-based dispersion, an emulsifier, a thickener, a film-forming aid and a leveling agent; wherein the PHA water-based dispersion is obtained by a PHA fermentation broth through a wall-breaking treatment. The present invention directly prepares the PHA water-based coating by using the PHA water-based dispersion obtained by fermentation and wall-breaking treatment upstream, and can prepare a PHA water-based coating that is not easy to settle, has good storage stability, is uniformly dispersed and has a small particle size.

The invention belongs to the technical field of preparation of microorganisms and cosmetics, and particularly relates to a strain composition, radix paeoniae rubra fermentation liquor prepared from the strain composition and application of the strain composition in cosmetics. Specifically, Pseudoalteromonas sp. SE9 and Schizoscharomyces pombe JM2-16 are utilized to perform fermentation treatment on radix paeoniae rubra root powder, and the finally obtained radix paeoniae rubra fermentation liquor has multiple effects of resisting oxidation, promoting proliferation and migration of keratinocytes, promoting skin injury repair, resisting allergy, resisting inflammation, relieving and the like, is high in safety, can be used as a cosmetic component, and can be used as a cosmetic preparation. Therefore, the method has good practical application value.

The invention provides a technical method for improving the anti-aging effect of eurotium cristatum tea compound fermentation liquor, and belongs to the technical field of microorganisms, the Latin name of eurotium cristatum is Eurotium cristatum, the eurotium cristatum is preserved in the China Center for Type Culture Collection, and the preservation number is CCTCC M 2024643. According to the method, the yeast seed solution is inoculated into the tea-sea buckthorn leaf culture solution fermented by the eurotium cristatum, so that the content of anti-aging active components such as procyanidine B2, quercitrin, cyanidin-3-glucoside, nicotinic acid, naringenin and rutin in the fermentation culture solution can be remarkably increased through co-fermentation, and the elastase inhibition rate can be remarkably increased; the anti-aging effect of a component system of the fermentation culture solution is enhanced. The prepared tea-hippophae rhamnoides leaf compound fermentation liquor is suitable for development of anti-aging cosmetics and has a wide market prospect.

The invention discloses a calendula double-bacterium fermentation product as well as a preparation method and application thereof, and relates to the technical field of plant fermentation. The preparation method of the calendula double-bacterium fermentation product disclosed by the invention comprises the following steps: carrying out primary fermentation by using lactobacillus plantarum, then carrying out secondary fermentation by using saccharomyces cerevisiae, and carrying out sterilization and filtration treatment on the calendula fermentation liquid subjected to secondary fermentation, so as to obtain the calendula fermentation liquid. The calendula fermentation liquor is subjected to combined fermentation through lactobacillus plantarum and saccharomyces cerevisiae, and the content of polyphenol and flavone in the calendula double-bacterium fermentation product is remarkably increased. Meanwhile, the calendula double-bacterium fermentation product has the effects of preserving moisture, resisting oxidation, relieving and controlling oil, and has a wide application prospect in the field of cosmetics.

The invention belongs to the technical field of medicinal and edible Chinese herbal medicine alcoholic drink, and particularly relates to lactobacillus plantarum and application thereof in fermentation of ginsenoside. The lactobacillus plantarum is specifically lactobacillus plantarum L100, and the preservation number of the lactobacillus plantarum L100 is CGMCC (China General Microbiological Culture Collection Center) No. 34425. The lactobacillus plantarum L100 has the function of converting and preparing the rare ginsenoside, basic components of ginseng can be directionally converted into high-value rare ginsenoside (such as Rg3, Rg5 and Rh1), and particularly, the lactobacillus plantarum L100 has extremely high specificity on generation of F5 and Rh10. Ginseng fermentation liquor obtained by fermenting ginseng through lactobacillus plantarum L100 and extracting solutions of medicinal and edible components such as codonopsis pilosula, astragalus membranaceus, liquorice and cistanche deserticola are used for preparing the liqueur, and a liqueur product with potential effects in the aspects of oxidation resistance, inflammation resistance, immunoregulation and the like is obtained.

The invention provides a streptomyces, which is named as Streptomyces sp. And is registered and preserved in Guangdong Microbial Culture Collection Center, and the preservation number is GDMCC No: 65917. The invention also provides a fermentation broth of the streptomyces, and an ethanol extract and a crude extract of the fermentation broth. The novel streptomyces, the crude extract thereof and the like have broad-spectrum antibacterial activity, have a good antagonistic effect on various pathogenic bacteria such as banana long leaf spot germs and banana colletotrichum gloeosporioides, can enable hyphae of the banana colletotrichum gloeosporioides to shrink, deform and break, inhibit spore germination of the banana colletotrichum gloeosporioides, and have a good anti-bacterial effect on the banana colletotrichum gloeosporioides. According to the present invention, with the application of the bacterial strain, the colletotrichum gloeosporioides spores are shrunk and broken, the growth of the colletotrichum gloeosporioides is inhibited, the peroxidase activity, the polyperoxidase activity, the CAT activity and the like in the banana peel can be improved, and the bacterial strain is the potential biological agent for the prevention and control of the colletotrichum gloeosporioides diseases, and has wide development space and good development and application prospects.

The invention provides a Bi-BDO fermentation pH dissolved oxygen dynamic optimization method based on an online Raman spectrum, belongs to the technical field of biological fermentation process control, and aims to solve the problems of unstable process and low efficiency caused by fermentation control lag and incapability of sensing the real metabolic state of cells in the prior art. The method comprises the following steps: acquiring the concentrations of a target product BDO and key byproducts such as acetic acid and ethanol in the fermentation liquor in real time through an online Raman spectrum; according to the method, a metabolic stress index is originally proposed and constructed, the index is obtained by performing weighted operation on the instantaneous generation rate of the by-product and the target product, and the index is used for quantitatively characterizing the intrinsic metabolic stress level of the cells in real time. The control strategy of maintaining the metabolic stress index in the preset optimal stable interval is taken as a core control strategy, the conversion from passive response to active prediction in the fermentation process is realized, and the yield, the stability and the batch repeatability of Bi-BDO production are remarkably improved.

The invention discloses banana resistant starch as well as a preparation method and application thereof in preparation of health-care food, and relates to the technical field of biology. The preparation method comprises the following steps: peeling immature bananas, performing irradiation disinfection, adding sterile water, performing grinding and pulping, and performing sieving with a 60-mesh sieve so as to obtain slurry; inoculating lactobacillus brevis and kluffa yeast into the slurry, and performing fermentation treatment to obtain fermentation liquor; standing the fermentation liquor, removing the supernatant, cleaning the precipitate with sterile water, and drying to obtain the banana resistant starch. The banana resistant starch prepared by performing combined fermentation treatment on lactobacillus brevis and Kluffa yeast can remarkably enhance the blood glucose metabolism regulation capability under the influence of high fat, so that the banana resistant starch can be applied to preparation of health-care food beneficial to maintaining the blood glucose health level. The method has important practical application prospects and is particularly suitable for market application and popularization.

The invention relates to the technical field of microorganisms, in particular to bacillus velezensis F0b and an application of the bacillus velezensis F0b. The bacillus velezensis F0b is preserved in the Guangdong Microbial Culture Collection Center on April 26, 2024, the preservation date of the bacillus velezensis F0b is April 26, 2024, and the preservation number of the bacillus velezensis F0b is GDMCC (China General Microbiological Culture Collection Center): 64566. The bacillus velezensis has prevention and / or control effects on pyricularia oryzae, cabbage sclerotinia sclerotiorum, tomato fusarium wilt, banana fusarium wilt, rice leaf spot, peronophythora litchii, soybean carbon rot, colletotrichum capsici and corn leaf curvularia, and can be used for preparing broad-spectrum microbial source bactericides. The fermentation liquor of the bacillus velezensis can generate amylase, carboxymethyl cellulose and protease, and the fermentation liquor of the bacillus velezensis has prevention and / or control effects on vegetable pathogenic fungal diseases, namely cabbage sclerotiniose or pepper anthracnose.

The preparation method comprises the following steps: mixing saccharomycetes, lactic acid bacteria and bacillus subtilis according to a ratio of 1: 1: 1 to obtain mixed bacteria; 0.25 g of mixed bacteria is taken and dissolved in 5 mL of sterile water for standby application; slicing and crushing the dried rhizoma polygonati, and sieving with a 10-mesh sieve to obtain rhizoma polygonati powder; the preparation method comprises the following steps: mixing water and rhizoma polygonati powder according to a liquid-material ratio of 10mL: 1g, sterilizing at 121 DEG C for 30min, cooling, adding mixed bacteria according to an inoculum size of 3% of the mass of rhizoma polygonati, culturing in a constant-temperature shaking table incubator at 120r. Min <-1 > for 72h at the culture temperature of 035 DEG C, sterilizing at 100 DEG C for 30min after fermentation, cooling and centrifuging to obtain rhizoma polygonati fermentation liquor, and storing at 4 DEG C. The prepared polygonatum sibiricum fermentation liquor is high in active ingredient content, has good cell safety, anti-oxidation, anti-inflammatory and whitening effects, and has the potential of being used for developing functional raw materials of traditional Chinese medicine cosmetics.

The invention provides a keto-alcohol acid reductoisomerase mutant and application of the keto-alcohol acid reductoisomerase mutant in construction of genetically engineered bacteria for high yield of D-pantothenic acid. In order to solve the technical problem that in the prior art, the yield of D-pantothenic acid synthesized through microbial fermentation is not high, semi-rational design of enzyme is adopted, and a keto-alcohol acid reductoisomerase mutant with substrate specificity is obtained and applied to construction of genetically engineered bacteria for high yield of D-pantothenic acid. According to the invention, a genetically engineered bacterium with high yield of D-pantothenic acid is constructed by utilizing the keto-alcohol acid reductoisomerase mutant through a CRISPR-Cas9 gene editing technology, so that the yield of D-pantothenic acid is increased by about 12.04%, and compared with an original strain, the valine accumulation amount in obtained fermentation liquor is almost unchanged compared with that of a control, and the yield of D-pantothenic acid is greatly improved. However, the accumulation of branched chain amino acid isoleucine is less, so that the modification of the substrate specificity of the keto-alcohol acid reductoisomerase is achieved.

The invention provides a preparation method of cereal fermentation broth postbiotics and application of the cereal fermentation broth postbiotics in improvement of systemic chronic inflammation and reduction of cholesterol and low-density lipoprotein, and belongs to the technical field of fermentation engineering. The invention provides a preparation method of cereal fermentation broth metabiotics, which is characterized in that mixed cereals are used as raw materials, anaerobic fermentation is carried out by using compound enzymes and various bacteria, and polysaccharides and proteins in the cereal raw materials can be quickly converted into functional small molecular substances, such as polypeptides, total phenols and total flavonoids. The cereal fermentation broth metagen can inhibit secretion of a macrophage proinflammatory medium, inhibit generation of inflammatory factors of liver, heart and intestinal tissues, and improve activation of adipose tissue macrophages related to body inflammation. After the cereal fermentation liquor is subjected to post-biogen treatment, glucose and lipid metabolism disorder can be effectively improved, liver function damage can be relieved, and the intestinal barrier function can be effectively improved.

The invention provides Yunnan golden camellia root endophytic fungi and application thereof, and relates to the technical field of crop disease control. The Yunnan camellia nitidissima root endophytic fungus is Lecanicillium vase JHC098 and is preserved in the China Center for Type Culture Collection, the preservation number is CCTCC NO: M 2025749, and a liquid fermentation liquor crude extract of the Lecanicillium vase can be applied to prevention and treatment of crop diseases. The invention overcomes the defects in the prior art, and the crude extract of the liquid fermentation liquor of the endophytic fungus Lecanicillium viculare JHC098 at the root of Yunnan golden camellia can effectively inhibit the growth of pathogenic bacteria such as citrus canker and tobacco bacterial wilt, thereby achieving the purpose of certain biological control.

The invention discloses burdock root fermentation liquor as well as a preparation method and application thereof in cosmetics, and belongs to the technical field of cosmetics. The preparation method provided by the invention comprises the following steps: inoculating a culture medium with pseudoalteromonas or schizosaccharomyces pombe for activation to obtain a seed solution; the seed solution is inoculated into a fermentation medium, burdock root powder is added, the adding concentration of the burdock root powder in the fermentation medium is 45-55 g / L, then fermentation is conducted to the end point, and after-treatment is conducted, the burdock root fermentation liquor is obtained. The burdock root fermentation liquor prepared by the preparation method disclosed by the invention has excellent anti-oxidation effect, anti-allergy soothing effect and tightening effect, also has certain healing promoting and repairing functions, is safe, non-toxic and small in irritation, and can be used for preparing anti-oxidation, anti-allergy and soothing cosmetic raw materials.

The invention belongs to the technical field of traditional Chinese medicine fermentation, and particularly relates to dendrobium officinale fermentation liquor with a pancreatic lipase inhibition effect and a preparation method of the dendrobium officinale fermentation liquor. The dendrobium officinale fermentation liquor is prepared by performing enzymolysis on dendrobium officinale and inoculating lactic acid bacteria for fermentation, wherein the dendrobium officinale fermentation liquor obtained by inoculating fermentation lactobacillus mucus DSM 13273 or leuconostoc mesenteroides subsp. Mesenteroides XD054 has a better pancreatic lipase activity inhibition effect.

Burkholderia, and a composition, use and usage method thereof. Provided are the Burkholderia, and a composition comprising the strain and / or a metabolite, culture, fermentation broth and / or extract thereof. The Burkholderia or the composition thereof can generate a siderophore, induce plants or seeds thereof to generate systemic resistance to plant pathogens, and reduce the chemotaxis of plant pathogens to plants, so that prevention and treatment of plant diseases and insect pests are achieved. In addition, plant root system development can be promoted, a nitrogen fixation effect is provided, a phosphate solubilization effect is provided, indoleacetic acid is generated, and weeds are removed, thereby promoting plant growth.

The invention provides a red ginseng processing method capable of increasing the content of rare ginsenoside and reducing pesticide residues, and relates to the technical field of medicinal material processing, the red ginseng processing method comprises the following steps: A, steaming fresh ginseng by using atomized probiotic fermentation liquor; b, drying the steamed fresh ginseng to obtain red ginseng; the pH (Potential of Hydrogen) of the probiotic fermentation liquor is 3.0 to 4.0; the OD600 value of the probiotic fermentation liquor is 1.2-2.5, the probiotic fermentation liquor is obtained through probiotic fermentation, the fermentation liquor with the pH value being 3.0-4.0 is adopted as an acid catalyst to replace purified water, in the ginseng steaming process, steaming is conducted through the atomized probiotic fermentation liquor, conversion of ginsenoside is promoted, and meanwhile organophosphorus pesticide residues in ginseng are degraded. The method is safe, efficient, green and natural, and loss of nutritional ingredients and functional ingredients caused by cooking is avoided. The technical problems that red ginseng processed and prepared in the prior art is low in rare saponin content and poor in effect and pesticide residues exist are solved.

The invention relates to the technical field of microorganisms, in particular to talaromyces purpurogenus, strain fermentation liquor of the talaromyces purpurogenus and application of the talaromyces purpurogenus to prevention and treatment of northern corn leaf blight. The invention provides biocontrol fungus fermentation liquor for preventing and treating northern corn leaf blight and application of the biocontrol fungus fermentation liquor. The preservation number of a strain of the biocontrol fungus fermentation liquor is CGMCC No.41911. The strain and the fermentation liquor thereof can completely inhibit various pathogenic bacteria, active substances in the fermentation liquor are relatively stable, the active validity period is long, the systemic property is relatively strong, the strain is not easy to degrade, high temperature resistance and microwave radiation resistance are realized, the antibacterial effect of chemical agents can be remarkably improved, and the effects of pesticide reduction and synergism are remarkably achieved when the strain and the fermentation liquor are mixed with chemical pesticides; therefore, the environmental safety problem and the drug resistance caused by chemical pesticides are obviously reduced.

The invention relates to cordyceps sinensis fermentation filtrate as well as a preparation method and application thereof. The preparation method comprises the following steps: (1) mixing cordyceps sinensis powder with an alcoholic solution, carrying out ultrasonic extraction, centrifuging to take supernate, and drying to obtain a cordyceps sinensis matrix extract; (2) mixing the cordyceps sinensis powder, the cordyceps sinensis matrix extract and a basic fermentation culture medium to obtain a main fermentation culture medium, and inoculating the main fermentation culture medium with a zymophyte solution for fermentation culture to obtain a fermentation solution; and centrifuging the fermentation liquor, filtering, and collecting the filtrate to obtain the cordyceps sinensis fermentation filtrate. By optimizing the fermentation process, the cordyceps sinensis powder and the cordyceps sinensis matrix extract obtained by ultrasonic extraction of the cordyceps sinensis powder are jointly fermented, so that the bioavailability of the cordyceps sinensis can be fully improved, the functional components in the cordyceps sinensis fermentation filtrate are enriched, and the health-care effect of the cordyceps sinensis is improved. The prepared cordyceps sinensis fermentation filtrate has an excellent anti-aging effect.

The invention discloses a whole corn plant harvesting, separating and packing machine, which belongs to the field of agricultural machinery and comprises a frame assembly, a corn header is arranged at the front end of the frame assembly, and a corn ear peeling device, a corn cob collecting device, a corn cob transferring device and a corn cob threshing device are arranged on the rear side of the corn header. A corn kernel storage chamber is arranged on the left side of the corn cob threshing device, and a corn cob transfer device and a corn cob storage chamber are arranged on the rear side of the corn cob threshing device; a straw smashing and screening device is arranged below the corncob peeling device, and a soft material conveying track, a hard skin conveying track, a soft material packaging machine, a film winding device, a fermentation liquor spraying device and a hard skin squeezing and bundling machine are arranged on the rear side of the straw smashing and screening device. Whole corn plants are harvested through one machine, and after being harvested, the whole corn plants are separated according to material attributes and are not wasted any more, so that the whole corn plants are fully utilized; people are intellectual; the effort is made to the best; the whole corn plants are completely eaten and squeezed on one machine.

Optimization of the formula of a culture medium for industrially producing a Bacillus velezensis TCS001 fermentation broth and culture process conditions therefor. By using the culture medium and the culture process conditions, not only can the fermentation production cost be reduced, but also the effective viable bacterium count and the spore count in the Bacillus velezensis TCS001 fermentation broth can be improved, so that the fermentation broth can be used for industrial production and market promotion and application. Provided is a lipopeptide-compound-containing mixture separated from a Bacillus velezensis TCS001 fermentation broth. By further optimizing the formula of the culture medium for the Bacillus velezensis TCS001 fermentation broth and the culture fermentation process conditions, the output and yield of lipopeptide compounds in the fermentation broth are successfully improved, providing an efficient and cost-effective solution for the control of plant diseases.

The present invention relates to a preparation method of a pine mushroom mycelium fermentation product, and its products and applications, including: inoculating pine mushroom liquid seeds into a culture medium for culturing, solid-liquid separation, obtaining pine mushroom mycelium and fermentation filtrate; subjecting the fermentation filtrate to alcohol precipitation treatment to obtain pine mushroom mycelium crude polysaccharide; inoculating fermentation bacteria seed liquid into a fermentation medium added with pine mushroom mycelium and pine mushroom mycelium crude polysaccharide for culturing to obtain a fermentation liquid; centrifuging the fermentation liquid, filtering the supernatant and sterilizing to obtain the pine mushroom mycelium fermentation product. The present invention develops a new fermentation product with pine mushroom mycelium as a fermentation substrate, the fermentation product has excellent soothing and antioxidant effects, and compared with the method of fermenting a single pine mushroom mycelium or a single pine mushroom mycelium crude polysaccharide, the fermentation product of the present invention has better soothing and antioxidant effects.

The invention belongs to the field of cosmetics, and discloses a composition with an anti-hair-loss effect and application thereof.The composition is prepared by mixing a cacumen biotae extract, a radix polygoni multiflori extract, an oil control factor and soybean milk fermentation liquor, and the anti-hair-loss effect of the cacumen biotae extract and the radix polygoni multiflori extract is further enhanced through the oil control factor and the soybean milk fermentation liquor; in addition, the invention further discloses a cosmetic which contains 1-10 wt% of the composition.

The invention relates to the technical field of daily chemicals, in particular to a preparation method and application of a sapindus mukorossi fermentation product with oil-controlling and dandruff-removing effects. The preparation method comprises the following steps: (1) drying soapberry in the air, separately powdering peel and seeds, adding 75-80 parts of water, performing ultrasonic extraction at 35 DEG C for 2 hours, then adding 0.5-0.9 part of dipotassium phosphate, sterilizing, and inoculating 3-5% of lactic acid bacteria liquid for fermentation; (2) after the fermentation is finished, adding 3-7 parts of sterilized aloe extract, 2-5 parts of mulberry leaf extract, 5-10 parts of tea bran extract and 6-9 parts of rose extract, adjusting the pH value to 5-7, inoculating 3-5% of schizosaccharomyces pombe, culturing for 24-36 hours at 28-30 DEG C, and introducing oxygen at 0.3-0.5 L / min; and (3) centrifuging at 10000r, and filtering through a sieve plate to obtain clear fermentation liquor, namely the sapindus mukorossi fermented product. The soapberry peel and the soapberry seeds are separately crushed, and the peel powder and the seed powder are mixed according to a ratio of 90%: 10% for use, so that the saponin content and the irritation are favorably controlled, and the use feeling is improved.

The present invention discloses a method for separating and purifying recombinant human collagen, wherein the method first filters the recombinant human collagen fermentation broth, then uses cationic composite chromatography to crudely purify the recombinant human collagen to obtain a cationic composite chromatography eluent, and then uses a hydrophobic chromatography column to purify the purified recombinant human collagen, wherein the cationic composite chromatography column is filled with a high-flow rate agarose matrix containing a hydrophobic group of a phenyl group and an electrostatic group of a carboxyl group as a filler, and the hydrophobic chromatography column is filled with a hydrophilic polymethacrylate matrix containing a phenyl group as a filler. The final target protein purity of the present invention is greater than 99%, the recovery rate is greater than 85%, and the endotoxin content is less than 0.5EU / mg. The process of the present invention is simple and easy to operate, and the purification cost requirement is low. The recombinant human collagen obtained by the method has high purity and low endotoxin content, and meets the requirements of cosmetic raw materials, medical devices, and medical-grade high-purity collagen.

The invention belongs to the technical field of modern agriculture, and particularly relates to a biocontrol bacillus amyloliquefaciens BA200 and application thereof. A new biological control bacterium BA200 is separated and identified, is named as bacillus amyloliquefaciens by taxonomy, and has a remarkable inhibition capability on two pathogenic bacteria, namely fusarium oxysporum and phytophthora vigna, causing the cowpea fusarium wilt, and the inhibition rate is up to 60% or above; fermentation liquor prepared from the bacillus amyloliquefaciens BA200 can remarkably reduce the morbidity and disease index of cowpea fusarium wilt, is a good biocontrol microorganism and has application and development potential and important significance in control of cowpea fusarium wilt, and the biocontrol bacterium bacillus amyloliquefaciens BA200 has a broad-spectrum biological control effect, can inhibit various phytopathogens and has a good application prospect. And valuable biocontrol bacterium resources are provided for preparation of biological bactericides.

The invention relates to the technical field of food, and particularly discloses a compound prebiotics embedded probiotic microcapsule and a preparation method and application thereof.The green tea leaves serve as main raw materials, water extraction is conducted on the green tea leaves, meanwhile, ultrasonic-assisted treatment is adopted, active ingredients in the green tea leaves can be more effectively released, the extraction time is shortened, and the content of probiotics in the green tea leaves is increased; the extraction rate of active ingredients in the green tea leaves is improved; then, lactobacillus acidophilus is adopted to ferment the extract liquid, so that the green tea fermentation liquid contains more antioxidant active ingredients, combined polyphenol in the extract liquid can release micromolecular polyphenol in the fermentation process, the content of substances such as flavone and polyphenol in the green tea fermentation liquid is increased, and the antioxidant activity is further improved; and then the modified dietary fiber is used as a main wall material of the microcapsule to embed the green tea fermentation liquor and the probiotics, so that the stability of active substances and the probiotics in the green tea fermentation liquor is improved, and the green tea fermentation liquor can play a role in protection and slow release in the simulated digestion process.

The invention belongs to the field of biological organic fertilizers, and discloses a special organic selenium-rich fertilizer for grapes as well as a preparation method and application thereof. The fertilizer is prepared from the following raw materials in percentage by mass: 0.05-0.2% of a selenium complex, 2-15% of a stabilizer, 15-25% of a microbial fermentation product, 0.5-2.0% of a natural plant extract and the balance of auxiliary materials, totaling 100%. The microbial fermentation product is microbial fermentation liquid obtained by fermenting bacillus tequilensis and cyanobacteria; the auxiliary material is obtained by mixing sodium alginate and maltodextrin; the natural plant extract is obtained by mixing azadirachtin, sophocarpidine, tea tree essential oil and cnidium lactone. Through the synergistic effect of the microbial fermentation product, the natural plant extract and the auxiliary materials, the industrial problem that in the prior art, a selenium fertilizer can only improve the selenium content of grapes, but cannot effectively improve the disease resistance and insect pest resistance of the grapes and slow down selenium element loss is solved, and the double improvement of the grape quality and the selenium content is achieved.