113 results about "Fungal pathogen" patented technology

The present invention is in the field of agricultural pathogen control. Methods and compositions are described for controlling plant diseases caused by pathogens, including fungi and oomycete phytopathogens. Chemicals were screened for activity as autophagy inhibitors. The screening method used a bioluminescence resonance energy transfer (BRET)-based high-throughput screening strategy to identify compounds that inhibit fungal ATG4 cysteine protease-mediated cleavage of ATG8 for autophagosome formation. Ebselen and ebselen analogs were identified as inhibitors of fungal pathogens ATG4-mediated ATG8 processing. Identified chemicals inhibit spore germination, hyphal development, and appressorium formation. The autophagy inhibitors significantly reduced the virulence of pathogens in their corresponding plant hosts. The invention also provides for compositions comprising the same, methods of making the same, and methods of controlling plant disease.

The present invention relates to a method of conferring or increasing resistance against fungal pathogens in plants and / or plant material. To this end the invention focuses on facilitating or increasing the production and / or accumulation of a membrane steroid binding protein 1 (MSBP1), fragment or homolog thereof in a plant or plant material compared to corresponding wild type plant or plant material. The invention also relates to plant and plant material having an increased resistance against fungal pathogens and to material and methods to create or use such plants plant parts or to produce products therefrom.

The invention provides a drug target protein MoFrd1 and application of the drug target protein MoFrd1 in agricultural disease control. The amino acid sequence of the MoFrd1 is as shown in SEQ ID No. 1, and the nucleotide sequence of the coding gene of the MoFrd1 is as shown in SEQ ID No. 4. Researches show that the pathogenicity of the magnaporthe oryzae can be remarkably reduced by knocking out the MoFrd1 coding gene. Homologous protein of MoFrd1 widely exists in plant pathogenic fungi and shows high conservative property in pathogenic mechanisms, however, homologous protein is not found in plants and mammals, and it is indicated that the homologous protein of MoFrd1 has the potential of serving as a specific drug target of pathogenic bacteria. Based on a key structural domain and a functional site of the MoFrd1 protein, a specific agent for preventing and treating plant fungal pathogens is expected to be developed through design or screening. The invention provides a new drug target and a control strategy for controlling fungal diseases of crops, and has important application value and market potential.

The invention relates to the technical field of in-vitro diagnosis, in particular to a primer probe combination for fungus PCR (polymerase chain reaction) detection. According to the invention, aiming at several detection targets of aspergillus, mucor, penicillium, blue marneffei and pneumocystis yarrowii, 18S rRNA, 5.8 S rRNA, 18S rRNA, ITS2 and a mitochondrial small subunit are respectively adopted as detection target regions for detection primer probe design, and different reaction systems are given for matching to complete automatic amplification. According to the primer probe combination and the kit for fungus PCR detection, the specificity for detecting different species of fungi is high, the detection system is stable, the detection limit is as low as 2 bacteria / mL, the joint detection design is beneficial to targeted coverage of fungal pathogens, and missing detection or mixed detection is effectively prevented.

The present invention includes compositions and methods of for treating plants infected with fungal pathogens by contacting an infected plant or plant at risk of infection with a fungicidal composition comprising an fungicide selected from copper compound such as copper octanoate or copper hydroxide, or a triazole fungicide such as myclobutanil, propionazole, tebuconazole or epoxiconazole, an enhancer selected from apyrase inhibitors, e.g., N-(m-tolyl)-[1,1′-biphenyl]-4-sulfonamide, S-heptyl 2-oxo-2H-chromene 3-carbothioate, 3-(N-(4-bromophenyl) sulfamoyl)-N-(3-nitrophenyl)benzamide, or (E)-3-methyl-N-(1-(naphthalen-2-yl) ethylidene)benzohydrazide and, optionally, a phytologically-acceptable inert carrier.

Described are immunogenic compositions comprising a non-naturally occurring pan-fungal Kex peptide, and methods of using such compositions for the treatment or prevention of infection and / or diseases associated with fungal pathogens (e.g., Pneumocystis, Aspergillus, Candida, or Cryptococcus) in the subject. Also provided are compositions and kits for detecting or quantifying the presence of antibodies directed against a non-naturally occurring pan-fungal Kex peptide in a subject.

The present invention relates to transfer DNAs which can be used to produce a genetically modified plant that only has DNA from that species of plant. The present invention also relates to genetically modified plants with a transfer DNA of the invention which has been processed during integration and methods of making such plants. Also provided is a DNA construct and methods of making a multigene construct comprising five genes which confer upon a plant enhanced resistance to a wide variety of fungal pathogens.

The invention belongs to the technical field of biological agriculture, and relates to bacillus licheniformis and application thereof. The strain disclosed by the invention has a good broad-spectrum antibacterial effect, has a good inhibition effect on various plant fungal pathogenic bacteria and bacterial pathogenic bacteria causing plant diseases, and particularly has a relatively good prevention and treatment effect on pumpkin anthracnose, and the prevention and treatment effect on the pumpkin anthracnose reaches 95% or above by using 10,000,000 cfu / mL of a microbial agent diluent; the prevention effect is obvious. The bacterial strain and the biological agent containing the bacterial strain can effectively overcome the defect that an existing product is single in control effect, and crop plant growth can be further promoted. And the bacillus licheniformis is collected from the natural environment, is safe, environment-friendly and pollution-free, and has a wide application prospect.

The subject of the present invention is a novel Paenibacillus polymyxa B134 strain, PCM deposit no. B / 00335, deposited in the Polish Collection of Microorganisms of the Polish Academy of Sciences in Wroclaw, and the use thereof in plant cultivation, for use against fungal pathogens and for biostimulation of plant growth and development.

Endophytic bacteria, compositions comprising the same, and methods of use thereof are disclosed which increase the root and shoot growth of grass hosts, suppress growth of soil borne fungal pathogens of host plants, increase resistance of the grass to diseases and reduce competitiveness of distantly related competitor weeds of the crop.

The disclosure relates to methods and compositions for altering the production of one or more secondary plant metabolites comprising applying an effective amount of at least one elicitor, wherein the elicitor is a jasmonate or a salicylate, and combinations thereof. The disclosure further teaches compositions comprising effective amounts of the elicitors disclosed here. The disclosure further relates to methods and compositions for controlling plant pathogens, such as fungal pathogens.

The present disclosure relates to a method for controlling phytopathogens including fungal pathogens. Particularly, the disclosure provides method of controlling fungal infestation in Soybean crop by application of fungicides. The disclosure also relates to corresponding use thereof.

The present invention is directed to stable flumetylsulforim compositions, combinations of fluxapyroxad and rapeseed oil, and to a method for the control and / or prevention of (i) fungal pathogen attack on a plant or (ii) plant and / or soil fungal disease, wherein the method comprises applying the composition or the combination described herein.

This invention provides compounds and methods of application that possess both broad-spectrum antifungal activity and plant-induced resistance, belonging to the field of agricultural technology. It addresses the problem that "traditional pure immune inducers must be applied before disease onset, making it difficult to meet the full-cycle control needs of disease prevention and treatment in the field." Compared to the previously developed PC1 and PC20, the two compounds of this invention, 2,4-dibromo-6-nitrophenol and 7-deazon-2'-deoxy-7-iodoadenosine, can both inhibit the growth of rice blast fungus and significantly improve rice resistance to rice blast through foliar spraying, meeting the full-cycle control needs of disease prevention and treatment in the field. Furthermore, these two compounds can directly inhibit the growth of various fungal pathogens such as peanut white mold, tomato gray mold, wheat scab, rice bakanae disease, rice false smut, mango malformation, and banana wilt.

The present application describes methods for disinfecting physical or biological surfaces by contact with antimicrobial formulations comprising substituted tolan compounds, such as one or more alkoxy (e.g., methoxy) and hydroxy substituents on one or two phenyl rings of the tolan. The compounds and methods are microbial and / or microbicidal, depending on the dose and suspected microorganism. Suspected pathogenic microorganisms include gram-negative bacteria, such as Pseudomonas aeruginosa (P.aeruginosa) and Escherichia coli (E.coli); gram-positive bacteria, such as Staphylococcus aureus (S.aureus), including MRSA; and fungal pathogens, such as Candida and Candida albicans (C.albicans). The present application also describes methods for inhibiting or disrupting biofilm formation of one or more microorganisms.

The present invention provides for an engineered, genetically modified, and / or synthetic plant receptor comprising a binding sequence that is capable of binding a fungal pathogen Avr ligand; wherein the non-engineered, genetically unmodified, and / or naturally occurring plant receptor does not bind the fungal pathogen Avr ligand, or bind with a lower binding affinity.

The present disclosure is directed to an approach using dsRNA to reduce or eliminate the production of DON by the fungal pathogen, F. graminearum or other fungal pathogens of the genus Fusarium and / or to control F. graminearum or other fungal pathogens of the genus Fusarium. In particular embodiments, methods and compositions are described to provide control of DON production and / or to control the fungal pathogen by causing mortality, suppression of growth, decrease in virulence or pathogenicity, or decrease in propagation / reproduction capacity (sporulation), by using exogenous dsRNA application administered to plants that are infected by or may become infected by F. graminearum or another member of the genus Fusarium that produces DON or otherwise is involved in Fusarium species complex that caused head blight.

Disclosed are compositions for competitive inhibition of pathogens against pathogenic infections comprising (a) Bacillus coagulans; (b) Bacillus subtilis; (c) citric acid; and (d) gallic acid including any combinations thereof. The compositions disclosed in this invention is capable to treat and prevent pathogenic infections of the genital organs as well as suitable for treatment and prevention of dermal infections. A method of preventing recurrence of the infections by way of supporting the growth of inherent natural doderlein microbial populations due to application of the compositions of the present invention are also disclosed. Further, use of the compositions of the present invention obviates the need of prior specific diagnosis of the infections due to its broad-spectrum activity against multiple bacterial and fungal pathogens.

The present invention provides a drug target protein MoFrd1 and its application in agricultural disease control. The amino acid sequence of MoFrd1 is shown in SEQ ID No.1, and the nucleotide sequence of its encoding gene is shown in SEQ ID No.4. Studies have shown that knocking out the MoFrd1 encoding gene significantly reduces the pathogenicity of rice blast fungus. Homologous proteins of MoFrd1 are widely present in plant pathogenic fungi and show high conservation in pathogenic mechanisms, but homologous proteins have not been found in plants and mammals, indicating its potential as a pathogen-specific drug target. Based on the key domains and functional sites of the MoFrd1 protein, it is expected to develop specific agents for controlling plant fungal pathogens through design or screening. The present invention provides new drug targets and control strategies for controlling fungal diseases of crops, and has important application value and market potential.

This invention relates to the fields of microbiology and biotechnology, and discloses Bacillus coagulans PPX-13 and its applications. Strain PPX-13 is a thermotolerant, multifunctional phosphate-solubilizing biocontrol bacterium that can reduce rhizosphere soil acidification in tea trees, improve fertility, enhance the microbial ecological environment, improve the functional microbial community and structure, inhibit mold communities and structure, and increase the yield of spring tea. It has been identified as Bacillus coagulans. This bacterium exhibits mesophilic and thermotolerant phosphate-solubilizing activity; phytase activity; multiple enzyme activities; and mesophilic antagonistic activity against isolates of anthracnose fungal pathogens. This strain holds promise for development into a novel agricultural microbial agent, which is of great significance for mitigating soil acidification, reducing fertilizer use, activating soil nutrients, improving soil quality, and increasing plant (especially tea) yield and quality.

The application discloses a method for synthesizing an antifungal pathogen reagent N-methyl-3-[(4-methylphenyl)thio]quinolinium iodide by realizing quinoline C3-H heteroatomization under nickel catalysis and mild conditions. The method comprises two steps, first, 3-(p-tolylthio)quinoline is synthesized under room temperature conditions, and then quinoline is N-methylated, so as to prepare the antifungal pathogen reagent N-methyl-3-[(4-methylphenyl)thio]quinolinium iodide. Compared with the past reports, the starting material is more cheap and easy to obtain, the steps are more simple, the atom utilization rate is higher, and the conditions are more mild, so the method is more suitable for kilogram scale amplification production.

A multifunctional agricultural composition is disclosed. The multifunctional agricultural composition combines organic insecticides, organic fertilizers, and organic fungicides for providing a cost-effective and environmentally sustainable solution for improving crop yield and health. The multifunctional agricultural composition is a versatile composition designed for universal crop applications that provides broad-spectrum pest control while delivering consistent performance across diverse crop varieties and environmental conditions. The multifunctional agricultural composition effectively targets a wide range of agricultural pests, fungal pathogens, and nutrient deficiencies, thereby making it suitable for use in varied farming systems, including horticulture, field crops, and orchard management.

The present invention relates to a method of conferring or increasing resistance to a plant and / or plant material against a fungal pathogen. To this end, the invention focuses on promoting or increasing the production and / or accumulation of Membrane Steroid Binding Protein 1 (MSBP1), fragments or homologues thereof in a plant or plant material as compared to the corresponding wild type plant or plant material. The invention also relates to plants and plant materials having increased resistance to fungal pathogens, as well as materials and methods of producing or using such plants, plant parts or products therefrom.

Described herein are fungicidal compositions and methods of controlling and / or suppressing phytopathogenic fungi and fungus-like pathogens and maintaining crop health. Particularly described are fungicidal compositions and methods of controlling the phytopathogenic fungi and the Aphanomyces fungus-like pathogen and maintaining crop health in crops susceptible to infection.

A system and methods for gas infusion tailored for manufacturing, prevention, and the application of fungicides in agriculture and aquaculture includes a microporous hollow fiber gas infusion module, engineered to efficiently dissolve gases such as oxygen or ozone into aqueous streams, thereby revolutionizing disease prevention and treatment strategies. Complementing the gas infusion module is a suite of manufacturing equipment and methodologies tailored for the production of oxygen-infused or other nutrient gas-infused products, catering to the diverse needs of agricultural and aquacultural industries. The system and method addresses the pervasive challenges posed by fungal pathogens in agriculture, such as Botrytis cinerea, Sclerotinia sclerotiorum, Pythium spp., and in aquaculture, particularly Saprolegnia, by offering comprehensive solutions encompassing precise gas infusion techniques, innovative disease prevention strategies, and sustainable manufacturing processes to enhance crop yields, promote environmental sustainability, and ensure the long-term viability of agricultural and aquacultural sectors.

In one aspect, the present application discloses compositions of extracellular vesicles (EVs) comprising heterologous effector RNAs, such as antifungal RNAs. In certain embodiments, the effector RNAs comprise dsRNA, while in other embodiments the effector RNAs comprise mRNA In certain embodiments, the EVs are plant EVs, microbial EVs (e.g., bacterial EVs or fungal EVs), or artificial EVs. In particular embodiments, the EVs are plant EVs and the effector RNA is mRNA. In other particular embodiments, the EVs are microbial EVs and the effector RNA is dsRNA. In other aspects, the application discloses microbes that produce RNA-loaded EVs; methods of treating and / or preventing fungal diseases by applying such microbes and / or RNA-loaded EVs to plants, plant parts, and / or soil; and methods of producing such RNA-loaded EVs. In certain examples, the antifungal RNAs may target DCL1 / 2 genes the VDS genes, and / or the tetraspanin (PLS1) genes of a fungal pathogen such as B. cinerea, Verticillium dahliae, Sclerotinia sclerotiorum, Fusarium oxysporum, Fusarium graminearum, Aspergillus niger or even oomycete pathogens, such as Phytophthora infestans.

Disclosed are compounds of Formula 1, including all geometric and stereoisomers, tautomers, N oxides, and salts thereof, wherein R1, R2, R3, R4, R5, R6, m and n are as defined in the disclosure. Also disclosed are compositions containing the compounds of Formula 1 and methods for controlling plant disease caused by a fungal pathogen comprising applying an effective amount of a compound or a composition of the invention.

A Capsicum plant resistant to powdery mildew caused by the fungal pathogen Leveillula taurica, a cell, plant part or seed of the Capsicum plant and to methods for identifying it, and the use of the plants for improving the yield of pepper production in an environment infected by Leveillula taurica and / or for limiting or controlling an infection by Leveillula taurica; wherein, the Capsicum plant includes introgressed in its genome a quantitative trait locus (QTL) conferring resistance to powdery mildew, wherein the QTL is located on chromosome 6 within the genomic interval delimited by the marker PE-0014628 (SEQ ID NO: 1) and the marker PE-0021476 (SEQ ID NO: 37).

The present invention relates to an emulsion concentrate (EC) formulation of succinate dehydrogenase inhibitor fungicides, the manufacture of such a formulation, and the use of its diluted form for the control of fungal pathogens, in particular in crops of useful plants. More specifically, the present invention relates to an emulsion concentrate of-difluoromethyl-1-methyl-1H-pyrazol-4-methoxyl-[1-methyl-2-(2, 4, 6-trichlorophenyl)-ethyl]-amide comprising a blend of at least three surfactants and an aromatic ester solvent.

TITLE: USE OF A BACILLUS SIAMENSIS STRAIN AS A BIOSOLUTION IN MUSHROOM CULTIVATION The present invention relates to the use of a particular bacterial species as a biosolution in the production and cultivation of mushrooms. More specifically, the invention relates to the use of a Bacillus siamensis strain, registered with the CNCM under CNCM order number I-5921, in stimulating the development and growth of a mycelium or fungus, improving the yields of edible mushroom crops, and controlling the main fungal pathogens of edible mushroom crops. [Fig. 1]