56 results about "Head blight" patented technology

The invention discloses a wheat single-ear gibberellic disease infection rate detection method in the technical field of intelligent agricultural information, and the method comprises the following steps: 1, optimizing a trunk feature extraction network in an AM-YOLOv7 network model; step 2, an activation function in the AM-YOLOv7 network model is optimized; step 3, a feature fusion pyramid network in the AM-YOLOv7 network model is optimized; step 4, the lightweight effect of the optimized AM-YOLOv7 network model is verified; step 5, carrying out transfer learning and model training on the optimized AM-YOLOv7 network model; and step 6, using the optimized AM-YOLOv7 network model to output a detection result. The method ensures that breeders perform real-time detection on the wheat single-ear scab infection rate in an actual scene, is suitable for edge equipment deployment, and solves the problem that the field detection requirement of the wheat single-ear scab infection rate in an actual disease-resistant breeding scene cannot be met due to deep network model hierarchy, large parameter quantity and high requirements on operation memory and computing power.

The invention relates to the technical field of biological control of plant diseases, in particular to application of a bacillus atrophaeus strain and a preparation thereof to control wheat gibberellic disease caused by gibberellic maize. The preservation number of the strain is CGMCC (China General Microbiological Culture Collection Center) No.29799, and the strain is classified and named as the bacillus atrophaeus strain HHQGTS13-1 (Bacillus atrophaeus HHQGTS13-1). The bacillus atrophaeus strain HHQGTS13-1 is wide in application range to natural environment conditions such as temperature and pH, and has the capability of antagonizing fusarium maydis when the temperature is 10-45 DEG C and the pH value is 4-10. The bacillus atrophaeus strain HHQGTS13-1 can be used for obviously inhibiting the growth of fusarium maydis hyphae and enabling the hyphae to be deformed; the antagonistic bacillus HHQGTS13-1 strain has an obvious inhibition effect on spore germination of fusarium maydis, the spore germination inhibition rate is 97.10%, the prevention and treatment effect of the antagonistic bacillus HHQGTS13-1 strain fermentation liquor after wheat is inoculated with fusarium maydis pathogenic bacteria is 63.43%, and the antagonistic bacillus HHQGTS13-1 strain has good potential of preventing and treating plant diseases caused by fusarium maydis.

This invention discloses the TaJDR1 gene and its application in improving plant resistance to Fusarium head blight and drought. The nucleotide sequence of the TaJDR1 gene is shown in SEQ ID NO.1, and its encoded amino acid sequence is shown in SEQ ID NO.2. This invention is the first to clone the TaJDR1 gene from the local wheat variety Wangshuibai and achieve overexpression of this gene in wheat using gene gun-mediated genetic transformation technology. The results show that the transgenic wheat exhibits significantly improved resistance to Fusarium head blight and drought resistance. Since the TaJDR1 gene originates from wheat itself, overexpression does not affect food safety; therefore, this invention can be widely applied in the field of disease and drought resistance breeding for various plants.

The present invention belongs to the field of molecular biology and genetic breeding technology, and discloses a molecular marker tightly linked to the wheat fusarium head blight resistance QTL qFHB3B.2 and its application. The present invention discovered a fusarium head blight resistance locus on the long arm of wheat chromosome 3B through genome-wide association analysis. <h2 style=";text-align:left;direction:ltr">qFHB3B.2 The closely linked peak SNP was further mapped to base 571,799,892 on chromosome 3B of the Chinese Spring wheat reference genome, explaining 5.4%-6.7% of the phenotypic variation. PARMS markers designed using this SNP were tested on 132 wheat varieties and found to be simple to use, providing clear typing and a good selection effect against wheat fusarium head blight.

The invention discloses a multiple KASP primer group capable of simultaneously detecting a wheat gibberellic disease-resistant gene Fhb7 and a wheat powdery mildew-resistant gene Pm21 and application of the multiple KASP primer group. The primer group is Multi-KASP AssayFhb7 and Pm21, and the primer group comprises primers of which the nucleotide sequences are as shown in SEQ ID NO. 1 to SEQ ID NO. 4. The invention further discloses a kit for detecting the content of the Pm21 in the KASP AssayFhb7 in the KASP AssayFhb7. According to the primer group system, efficient joint detection of two important disease-resistant genes, namely, the wheat scab-resistant gene Fhb7 and the wheat powdery mildew-resistant gene Pm21, in single-tube and single-time reaction is realized for the first time, and the primer group system has the advantages of simplicity and convenience in operation, high flux, strong specificity, visual result and the like. The primer group is suitable for rapid and accurate identification of excellent genotypes in a large-scale genetic group in a wheat disease-resistant molecule pyramiding breeding process, improves the synergistic improvement efficiency of wheat scab and powdery mildew, can also assist in precise and efficient integration of the excellent disease-resistant genotypes and other excellent characters or target genes, shortens the breeding period, and has a good application prospect in the field of wheat disease-resistant molecule pyramiding breeding. The breeding of a new wheat variety with strong resistance and excellent comprehensive characters is accelerated.

The present disclosure is directed to an approach using dsRNA to reduce or eliminate the production of DON by the fungal pathogen, F. graminearum or other fungal pathogens of the genus Fusarium and / or to control F. graminearum or other fungal pathogens of the genus Fusarium. In particular embodiments, methods and compositions are described to provide control of DON production and / or to control the fungal pathogen by causing mortality, suppression of growth, decrease in virulence or pathogenicity, or decrease in propagation / reproduction capacity (sporulation), by using exogenous dsRNA application administered to plants that are infected by or may become infected by F. graminearum or another member of the genus Fusarium that produces DON or otherwise is involved in Fusarium species complex that caused head blight.

The invention belongs to the technical field of genetic engineering and biology, and relates to application of trichoderma harzianum TrVPT1 in regulation and control of trichoderma phosphorus accumulation and gibberellic disease resistance. The nucleotide sequence of the trichoderma harzianum vacuole phosphate transporter gene TrVPT1 is as shown in SEQ ID NO: 1. The invention proves that the TrVPT1 gene has an important regulation and control effect in trichoderma phosphorus steady-state regulation and control and biological prevention and control functions.

The invention discloses a protein AP23 with an amino acid sequence as shown in SEQ ID NO: 1, which can be combined with fusarium A to improve the disease resistance of wheat to gibberellic disease and basal stem rot, and has important significance for cultivating new disease-resistant plant varieties resistant to fusarium graminearum infection.

This invention relates to the field of biotechnology, and more particularly to the application of the wheat molecular chaperone protein TaBiP1 in the control of wheat scab. This invention is the first to discover that overexpression of the wheat molecular chaperone protein TaBiP1 can significantly reduce the infection severity of wheat scab. Compared with the transgenic recipient parent Fielder, TaBiP1-overexpressing plants significantly reduce wheat scab infection and can be used to create new scab-resistant germplasm. In this invention, the TaBiP1 gene in recipient wheat is edited using CRISPR / Cas9 gene editing technology to obtain transgenic plants. bip1 The resistance of wheat to Fusarium head blight was weaker than that of Fielder, which confirms the positive significance of TaBiP1 in enhancing wheat resistance to Fusarium head blight.

This invention belongs to the field of wheat breeding, specifically relating to a wheat Fusarium head blight resistance locus and molecular breeding method. This invention identified five stable Fusarium head blight resistance-associated loci, Qfhb.jaas-2D, Qfhb.jaas-3A, Qfhb.jaas-3B.1, Qfhb.jaas-5A.2, and Qfhb.jaas-6D, on chromosomes 2A, 2D, 3A, 5A, and 6D. For the identified resistance loci, this invention designed KASP molecular markers. These markers have high specificity and sensitivity, enabling accurate and rapid detection of resistance genotypes in wheat materials, providing a powerful tool for marker-assisted selection (MAS). This invention verified the effectiveness of the screened resistance loci. The results showed that the above five loci were significantly associated with Fusarium head blight resistance, providing a reliable basis for the breeding of resistant varieties.

The invention discloses a KASP primer group for detecting a gibberellic disease resistance gene and application of the KASP primer group, and belongs to the technical field of wheat molecular breeding. According to the present invention, the Fhb7Th1 gene and the Fhb7Thp gene from the decaploid elytrigia elongata and the diploid elytrigia elongata are analyzed, and the result shows that the Fhb7Th1 gene and the Fhb7Thp gene have an A / C polymorphic site at the 383 nucleotide, and can be adopted as the gibberellic disease resistance site; based on the gibberellic disease-resistant site, the invention designs the KASP primer group for detecting the gibberellic disease-resistant gene, and the rapid and accurate detection of the resistance genes Fhb7Th1 gene and Fhb7Thp gene can be realized, so that the pyramiding breeding of the wheat gibberellic disease-resistant gene can be realized, and the KASP primer group has important significance for improving the resistance of wheat to gibberellic disease.

This invention discloses a molecular breeding method for creating new wheat germplasm highly resistant to Fusarium head blight, stripe rust, and leaf rust. This method first develops newly located loci for grain weight / grain length / grain width. QTGW / KL / KW-1A The linkage marker KASP.QTGW / KL / KW-1A was identified and its effectiveness was verified. A major active site for resistance to Fusarium head blight was also developed. QFhb-2DL The linkage markers Fhb-2D-dcaps for the candidate genes were used. During the breeding process, molecular marker selection was first used to ensure that the created DH lines carried... QFhb-2DL Candidate genes for resistance to Fusarium head blight and QTGW / KL / KW-1A The enhanced allelic variation ensures the basic resistance and yield traits of the bred germplasm or materials. Furthermore, after propagation of the DH line, phenotypic identification was conducted in Yangzhou and Chengdu, simultaneously screening for resistance to Fusarium head blight, stripe rust, and leaf rust. Using local yield control varieties as comparisons, this facilitates the selection of germplasm or varieties adapted to local conditions and resistant to both Fusarium head blight and stripe rust / leaf rust, achieving a breakthrough in disease-resistant wheat breeding in the middle and upper reaches of the Yangtze River.

This invention discloses a KASP primer set related to wheat scab resistance and its application, belonging to the field of crop breeding; the primer set contains specific primers with nucleotide sequences as shown in SEQ ID NO.1, SEQ ID NO.2 and SEQ ID NO.3 respectively; this KASP primer set can target molecular markers located on wheat chromosome 3A. Kfhb-3A Genotyping was performed, and experiments showed that wheat samples with the SNP locus genotype T exhibited statistically superior resistance to Fusarium head blight compared to wheat samples with the genotype C. This KSAP primer set can be used for marker-assisted selection breeding to improve the efficiency of Fusarium head blight resistance breeding.

The present disclosure belongs to the field of genetic engineering technology and relates to a voltage-dependent anion channel gene TaVDAC1 of wheat and use thereof. The wheat voltage-dependent anion channel gene TaVDAC1 negatively regulates wheat resistance to Fusarium head blight (FHB). When the gene TaVDAC1 is overexpressed, the resistance of wheat to FHB decreases; conversely, when the gene TaVDAC1 is silenced or edited, the resistance of wheat to FHB significantly increases. The wheat voltage-dependent anion channel gene TaVDAC1 may be used for the breeding and development of crop variety or germplasm resources with resistance to FHB, thereby enhancing wheat disease resistance and addressing the technical issues of wheat susceptibility to FHB and the reliance on chemical control methods for its management.

This invention relates to the KASP molecular marker for wheat Fusarium head blight resistance QTL Qfhb.sdau-4AL and its application, belonging to the field of molecular marker technology for wheat Fusarium head blight resistance. Using BSR-Seq analysis, this invention located a novel QTL locus for wheat Fusarium head blight resistance on chromosome 4AL (738-740 Mb), named Qfhb.sdau-4AL. From this QTL interval, a KASP molecular marker was developed, named KASP-QFhb.sdau-4A.2, with a physical location of 738606950. This molecular marker shows a high correlation with Fusarium head blight resistance and can be applied to marker-assisted selection breeding. Therefore, this invention uncovers a new Fusarium head blight resistance gene, providing new gene resources for the breeding of new Fusarium head blight resistant wheat varieties.

Provided are a fungicidal composition comprising bixafen and a use thereof. The fungicidal composition comprises the following components in percentage by weight: 3-10% of bixafen, 5-20% of a triazole fungicide, 8-12% of Termul 5030, 1-3% of Atlox 4912, 1-5% of ethylene glycol, 13-18% of cyclohexanone, and deionized water to make up 100%. The triazole fungicide is tebuconazole or prothioconazole. A prepared microemulsion has excellent formulation performance, emulsifiers are compounded to form dense micelles so as to increase the solubilization capacity and reduce the amount of emulsifiers, thereby reducing production costs, and the microemulsion has an excellent control effect on wheat fusarium head blight.

This invention discloses the application of the wheat gene TaMIPS in regulating resistance to Fusarium head blight and stem rot. Through the creation of wheat materials, it was demonstrated that overexpression of the TaMIPS gene significantly enhances wheat resistance to Fusarium head blight and stem rot, while mutants exhibit decreased disease resistance. Furthermore, this invention utilizes gene editing technology to optimize the promoter region of the TaMIPS gene, removing the inhibitory effect of the upstream non-coding open reading frame (uORF) on its expression, thereby significantly increasing the expression level of the TaMIPS gene-encoded protein and effectively enhancing wheat disease resistance.

The invention provides application of streptoamycin in inhibiting growth of plant pathogenic fungus hyphae and preventing diseases of plants, and belongs to the technical field of pesticides. Streptoamycin can inhibit cucumber anthracnose pathogenic fungi, rice bakanae disease pathogenic fungi, rice damping off pathogenic fungi, rice blast pathogenic fungi, wheat scab pathogenic fungi, pumpkin gummy stem blight pathogenic fungi, pawpaw anthracnose pathogenic fungi, cucumber corynespora disease pathogenic fungi and wheat root rot pathogenic fungi. Growth of hyphae of plant pathogenic fungi such as mango anthrax pathogenic fungi or corn smut pathogenic fungi. The bactericidal composition can also be used for preventing and treating cucumber anthracnose, rice bakanae disease, rice damping-off, rice blast and the like. In addition, the invention provides a reagent which contains the streptoamycin and is used for inhibiting the plant pathogenic fungi, so that the application field of the streptoamycin is enriched, and the variety of plant antibiotics is also enriched.

The invention discloses application of a wheat TaRFS4B gene in synergistically improving wheat scab and banded sclerotial blight resistance. The nucleotide sequence of the TaRFS4B gene is as shown in SEQ ID No.1, and the nucleotide sequence of a coding region is as shown in SEQ ID No.2. The invention further discloses application of the TaRFS4B gene in synergistically improving wheat scab and banded sclerotial blight resistance. The over-expression of the TaRFS4B gene can improve the resistance of wheat to gibberellic disease and banded sclerotial blight at the same time, provides an effective technical means for prevention and control of the two diseases in production, further accelerates the breeding process of wheat disease resistance, perfects the prevention and control measures of the diseases, and has a wide application prospect.

The invention discloses a thiophanate-methyl-metconazole suspending agent for treating wheat scab and a preparation method of the thiophanate-methyl-metconazole suspending agent, and belongs to the technical field of pesticide preparations. The thiophanate-methyl-metconazole suspending agent is prepared from the following raw materials: 34 to 36 weight percent of thiophanate-methyl, 4 to 6 weight percent of metconazole, 3 to 5 weight percent of a dispersing agent, 3 to 5 weight percent of an anti-freezing agent, 0.1 to 0.3 weight percent of a de-foaming agent, 0.2 to 0.3 weight percent of a preservative, 0.2 to 0.3 weight percent of magnesium aluminum silicate, 0.1 to 0.3 weight percent of xanthan gum, 2 to 3 weight percent of a functional additive and the balance of water, wherein the dispersing agent is a hydrophobic modified carboxylic acid copolymer type dispersing agent; the defoaming agent is an organic silicon defoaming agent; the functional additive is prepared from a bio-based fluorosilicone copolymer, porous microspheres, alkyl glycoside and nano titanium dioxide. The thiophanate-methyl-metconazole suspending agent prepared by the invention has the advantages of high efficiency, environmental protection and stable dosage form, and can effectively prevent and treat wheat scab, reduce the use amount of pesticide, reduce the drug resistance and improve the safety of crops.

The invention belongs to the technical field of pesticides, and particularly relates to a pyrrole compound and application thereof. The compound is shown as a general formula I: # imgabs0 #, wherein X represents hydrogen, halogen, alkyl, alkenyl, alkynyl and the like; y represents hydrogen, halogen, cyano, trialkylsilyl, alkyl or the like; z represents an aryl group or a heterocyclic group. The compound has excellent prevention and treatment effects on pests and / or fungi (especially nematode, gibberellic disease, banded sclerotial blight, gray mold and anthracnose).

The invention discloses a gibberellic disease resistance related receptor protein kinase gene as well as an expression vector and application thereof, and belongs to the field of gene engineering. The cDNA (complementary deoxyribonucleic acid) sequence of the TaRLK3-3B is SEQ ID NO. 1, and the amino acid sequence coded by the TaRLK3-3B is SEQ ID NO. 2. The gene is inserted into an expression vector pAHC25 to obtain an overexpression vector of the gene, and the overexpression vector of the gene is introduced into an infected wheat variety, so that the resistance of the wheat variety infected with the gibberellic disease to the gibberellic disease can be remarkably improved. The TaRLK3-3B is used for genetic engineering breeding, and when the TaRLK3-3B is introduced into wheat varieties susceptible to gibberellic disease, the gibberellic disease resistance of wheat can be improved.

The invention discloses an application of a terpenoid natural product containing an isoprene unit as an agricultural bactericide in plant diseases, the terpenoid natural product containing the isoprene unit is composed of two or more isoprene units, and the terpenoid natural product containing the isoprene unit aims at plant viral diseases such as tobacco mosaic virus, cucumber mosaic virus or potato Y virus; aiming at plant fungal diseases such as cucumber gray mold, pepper fusarium wilt, sclerotinia rot of colza, wheat scab, potato late blight, blueberry root rot, botryosphaeria dothidea, pitaya anthracnose, rice sheath blight disease, fusarium SP or colletotrichum capsici; the compound has a good inhibition effect on plant bacterial diseases such as rice bacterial leaf blight, citrus canker, tobacco bacterial wilt, kiwifruit canker or rice bacterial leaf streak. The invention provides new application of terpenoid natural products, and the terpenoid natural products can be used for preventing and treating plant bacterial diseases, fungal diseases and plant viral diseases in the agricultural field.

The invention discloses a wheat scab resistance QTL molecular marker and application thereof, SNP molecular markers are located at 524bp, 519bp and 822bp of a wheat 2D chromosome long arm, and SNP allelic variation is C / T. The gibberellic disease-resistant QTL detection molecular marker can distinguish favorable and unfavorable allelic variations through genotypes, and can save a large amount of manpower, material resources and time for breeding of gibberellic disease-resistant wheat varieties. According to the enzyme digestion amplification polymorphic sequence marker for detecting the wheat gibberellic disease-resistant QTL locus, PCR, restriction enzyme and electrophoresis technologies are utilized to select SNP allelic variation genotypes associated with the wheat gibberellic disease-resistant QTL so as to achieve the purpose of selecting and breeding a gibberellic disease-resistant variety under the assistance of a molecular marker. The marker can show stable and clear stripes in a breeding population composed of 469 natural varieties, and has the advantages of good stability, high accuracy and the like.

The present invention discloses molecular markers for detecting the Fusarium head blight resistance QTL QFhb-yaas-4AL and their applications. Genotype data are obtained by using a Wheat16K high-throughput wheat gene chip, and a QTL locus QFhb-yaas-4AL related to Fusarium head blight resistance derived from resistant materials is detected. One KASP marker and its detection primer set are developed to efficiently screen for high or low Fusarium head blight resistance. By performing PCR amplification on wheat genomic DNA with the primer set of the present invention, it is possible to directly determine whether the gene for high Fusarium head blight resistance of the resistant material is carried through KASP genotyping. The detection method is simple to operate, the detection result is very intuitive, the detection effect is significantly effective, and the use of this molecular marker for screening can greatly improve the efficiency of molecular marker-assisted selection for wheat breeding with high or low Fusarium head blight resistance.

The present application is suitable for the technical field of sorghum fusarium head blight identification, and provides a molecular marker for identifying sorghum resistance or susceptibility to fusarium head blight, comprising a molecular marker 6Sam72772 and / or Tx430508; the molecular marker 6Sam72772 can be a 126bp DNA fragment and a 122bp DNA fragment, the nucleotide sequence of the 126bp DNA fragment is shown as SEQ ID:5, and the nucleotide sequence of the 122bp DNA fragment is shown as SEQ ID:6; the molecular marker Tx430508 is a 720bp DNA fragment, and the nucleotide sequence is shown as SEQ ID:7. The present application also provides related primer groups, kits, and methods for identifying sorghum resistance or susceptibility to fusarium head blight. The present application provides the molecular markers 6Sam72772 and Tx430508, which can accurately identify or assist in identifying sorghum resistance or susceptibility to fusarium head blight.

A method for improving wheat resistance to Fusarium head blight (FHB) by genome editing is provided, and further provided is use of TaCIPK14 protein or related biomaterial thereto in the regulation of plant resistance to FHB; the TaCIPK14 protein is TaCIPK14-4A protein (SEQ ID NO: 4), TaCIPK14-4B protein (SEQ ID NO: 5), and / or TaCIPK14-4D protein (SEQ ID NO: 6). TaCIPK14 knockout mutant plants are successfully obtained by simultaneously editing three alleles of TaCIPK14 in wheat using CRISPR-Cas9 gene editing technology. The TaCIPK14 knockout mutant shows resistance to inoculation with FHB pathogens, and the results of evaluation of major agronomic traits confirm that the TaCIPK14 knockout mutant reserves the major agronomic traits.

This invention provides a method for breeding disease-resistant wheat varieties. [Solution] The wheat receptor-like protein kinase TaMLR1 provided by this application has a specific amino acid sequence, and the open reading frame (ORF) sequence encoding the wheat receptor-like protein kinase TaMLR1 is described in a specific nucleic acid sequence. In this disclosure, gene editing is performed on wild-type material Fielder to obtain TaMLR1 gene-edited mutant plants exhibiting resistance to wheat yellow rust, powdery mildew, and Fusarium head blight. This disclosure provides a novel technical approach for breeding disease-resistant varieties, confirms the negative regulatory role of this gene in wheat disease resistance, and provides a novel genetic resource for broad-spectrum disease-resistant breeding of wheat.

This invention discloses a 1-(2-aminobenzyl)-3-arylurea compound, its preparation method, and its uses. This type of compound is synthesized from o-aminobenzylamine and aryl isocyanate compounds as raw materials, using triethylamine as an acid-binding agent and dichloromethane as a solvent via a condensation reaction in an ice-water bath. The raw materials used in the synthesis of this type of compound are inexpensive and readily available, and the synthesis method is simple. It exhibits good inhibitory effects on crop pathogens, particularly showing significant inhibitory activity against Sclerotinia sclerotinia, Phytophthora blight, Fusarium head blight, Carbendazim, Botrytis cinerea, and Sheath blight.

The invention discloses a molecular design breeding method for rapidly improving wheat scab and powdery mildew resistance based on multi-gene polymerization, which comprises the following steps: taking a scab-resistant material NMAS084 which carries a plurality of scab-resistant genes and has excellent comprehensive agronomic characters as a recurrent parent, taking Yangmai 158 near-isogenic line Y7A2033 containing a powdery mildew-resistant gene Mlm2033 as a receptor parent, and carrying out multi-gene polymerization on the basis of the recurrent parent; a linkage marker M1 of a molecular marker powdery mildew resistance gene Mlm2033, a linkage marker M2 of Fhb1, a linkage marker M3 and M4 of Fhb2, a linkage marker M5 of Fhb4 and a linkage marker M6 of Fhb5 are used for auxiliary selection, and a gibberellic disease expansion resistance identification technology is combined, so that a new wheat germplasm with good adaptability, excellent comprehensive characters and resistance to gibberellic disease and powdery mildew is created; the method is of great significance to cultivation of wheat germplasm with wide spectrum-resistant range, stable and lasting disease resistance and excellent comprehensive characters.