29 results about "Head blight" patented technology

This invention discloses the TaJDR1 gene and its application in improving plant resistance to Fusarium head blight and drought. The nucleotide sequence of the TaJDR1 gene is shown in SEQ ID NO.1, and its encoded amino acid sequence is shown in SEQ ID NO.2. This invention is the first to clone the TaJDR1 gene from the local wheat variety Wangshuibai and achieve overexpression of this gene in wheat using gene gun-mediated genetic transformation technology. The results show that the transgenic wheat exhibits significantly improved resistance to Fusarium head blight and drought resistance. Since the TaJDR1 gene originates from wheat itself, overexpression does not affect food safety; therefore, this invention can be widely applied in the field of disease and drought resistance breeding for various plants.

The present disclosure is directed to an approach using dsRNA to reduce or eliminate the production of DON by the fungal pathogen, F. graminearum or other fungal pathogens of the genus Fusarium and / or to control F. graminearum or other fungal pathogens of the genus Fusarium. In particular embodiments, methods and compositions are described to provide control of DON production and / or to control the fungal pathogen by causing mortality, suppression of growth, decrease in virulence or pathogenicity, or decrease in propagation / reproduction capacity (sporulation), by using exogenous dsRNA application administered to plants that are infected by or may become infected by F. graminearum or another member of the genus Fusarium that produces DON or otherwise is involved in Fusarium species complex that caused head blight.

The invention discloses a protein AP23 with an amino acid sequence as shown in SEQ ID NO: 1, which can be combined with fusarium A to improve the disease resistance of wheat to gibberellic disease and basal stem rot, and has important significance for cultivating new disease-resistant plant varieties resistant to fusarium graminearum infection.

This invention relates to the field of biotechnology, and more particularly to the application of the wheat molecular chaperone protein TaBiP1 in the control of wheat scab. This invention is the first to discover that overexpression of the wheat molecular chaperone protein TaBiP1 can significantly reduce the infection severity of wheat scab. Compared with the transgenic recipient parent Fielder, TaBiP1-overexpressing plants significantly reduce wheat scab infection and can be used to create new scab-resistant germplasm. In this invention, the TaBiP1 gene in recipient wheat is edited using CRISPR / Cas9 gene editing technology to obtain transgenic plants. bip1 The resistance of wheat to Fusarium head blight was weaker than that of Fielder, which confirms the positive significance of TaBiP1 in enhancing wheat resistance to Fusarium head blight.

This invention belongs to the field of wheat breeding, specifically relating to a wheat Fusarium head blight resistance locus and molecular breeding method. This invention identified five stable Fusarium head blight resistance-associated loci, Qfhb.jaas-2D, Qfhb.jaas-3A, Qfhb.jaas-3B.1, Qfhb.jaas-5A.2, and Qfhb.jaas-6D, on chromosomes 2A, 2D, 3A, 5A, and 6D. For the identified resistance loci, this invention designed KASP molecular markers. These markers have high specificity and sensitivity, enabling accurate and rapid detection of resistance genotypes in wheat materials, providing a powerful tool for marker-assisted selection (MAS). This invention verified the effectiveness of the screened resistance loci. The results showed that the above five loci were significantly associated with Fusarium head blight resistance, providing a reliable basis for the breeding of resistant varieties.

The invention discloses a KASP primer group for detecting a gibberellic disease resistance gene and application of the KASP primer group, and belongs to the technical field of wheat molecular breeding. According to the present invention, the Fhb7Th1 gene and the Fhb7Thp gene from the decaploid elytrigia elongata and the diploid elytrigia elongata are analyzed, and the result shows that the Fhb7Th1 gene and the Fhb7Thp gene have an A / C polymorphic site at the 383 nucleotide, and can be adopted as the gibberellic disease resistance site; based on the gibberellic disease-resistant site, the invention designs the KASP primer group for detecting the gibberellic disease-resistant gene, and the rapid and accurate detection of the resistance genes Fhb7Th1 gene and Fhb7Thp gene can be realized, so that the pyramiding breeding of the wheat gibberellic disease-resistant gene can be realized, and the KASP primer group has important significance for improving the resistance of wheat to gibberellic disease.

This invention discloses a molecular breeding method for creating new wheat germplasm highly resistant to Fusarium head blight, stripe rust, and leaf rust. This method first develops newly located loci for grain weight / grain length / grain width. QTGW / KL / KW-1A The linkage marker KASP.QTGW / KL / KW-1A was identified and its effectiveness was verified. A major active site for resistance to Fusarium head blight was also developed. QFhb-2DL The linkage markers Fhb-2D-dcaps for the candidate genes were used. During the breeding process, molecular marker selection was first used to ensure that the created DH lines carried... QFhb-2DL Candidate genes for resistance to Fusarium head blight and QTGW / KL / KW-1A The enhanced allelic variation ensures the basic resistance and yield traits of the bred germplasm or materials. Furthermore, after propagation of the DH line, phenotypic identification was conducted in Yangzhou and Chengdu, simultaneously screening for resistance to Fusarium head blight, stripe rust, and leaf rust. Using local yield control varieties as comparisons, this facilitates the selection of germplasm or varieties adapted to local conditions and resistant to both Fusarium head blight and stripe rust / leaf rust, achieving a breakthrough in disease-resistant wheat breeding in the middle and upper reaches of the Yangtze River.

This invention discloses a KASP primer set related to wheat scab resistance and its application, belonging to the field of crop breeding; the primer set contains specific primers with nucleotide sequences as shown in SEQ ID NO.1, SEQ ID NO.2 and SEQ ID NO.3 respectively; this KASP primer set can target molecular markers located on wheat chromosome 3A. Kfhb-3A Genotyping was performed, and experiments showed that wheat samples with the SNP locus genotype T exhibited statistically superior resistance to Fusarium head blight compared to wheat samples with the genotype C. This KSAP primer set can be used for marker-assisted selection breeding to improve the efficiency of Fusarium head blight resistance breeding.

This invention relates to the KASP molecular marker for wheat Fusarium head blight resistance QTL Qfhb.sdau-4AL and its application, belonging to the field of molecular marker technology for wheat Fusarium head blight resistance. Using BSR-Seq analysis, this invention located a novel QTL locus for wheat Fusarium head blight resistance on chromosome 4AL (738-740 Mb), named Qfhb.sdau-4AL. From this QTL interval, a KASP molecular marker was developed, named KASP-QFhb.sdau-4A.2, with a physical location of 738606950. This molecular marker shows a high correlation with Fusarium head blight resistance and can be applied to marker-assisted selection breeding. Therefore, this invention uncovers a new Fusarium head blight resistance gene, providing new gene resources for the breeding of new Fusarium head blight resistant wheat varieties.

Provided are a fungicidal composition comprising bixafen and a use thereof. The fungicidal composition comprises the following components in percentage by weight: 3-10% of bixafen, 5-20% of a triazole fungicide, 8-12% of Termul 5030, 1-3% of Atlox 4912, 1-5% of ethylene glycol, 13-18% of cyclohexanone, and deionized water to make up 100%. The triazole fungicide is tebuconazole or prothioconazole. A prepared microemulsion has excellent formulation performance, emulsifiers are compounded to form dense micelles so as to increase the solubilization capacity and reduce the amount of emulsifiers, thereby reducing production costs, and the microemulsion has an excellent control effect on wheat fusarium head blight.

This invention discloses the application of the wheat gene TaMIPS in regulating resistance to Fusarium head blight and stem rot. Through the creation of wheat materials, it was demonstrated that overexpression of the TaMIPS gene significantly enhances wheat resistance to Fusarium head blight and stem rot, while mutants exhibit decreased disease resistance. Furthermore, this invention utilizes gene editing technology to optimize the promoter region of the TaMIPS gene, removing the inhibitory effect of the upstream non-coding open reading frame (uORF) on its expression, thereby significantly increasing the expression level of the TaMIPS gene-encoded protein and effectively enhancing wheat disease resistance.

The invention discloses application of a wheat TaRFS4B gene in synergistically improving wheat scab and banded sclerotial blight resistance. The nucleotide sequence of the TaRFS4B gene is as shown in SEQ ID No.1, and the nucleotide sequence of a coding region is as shown in SEQ ID No.2. The invention further discloses application of the TaRFS4B gene in synergistically improving wheat scab and banded sclerotial blight resistance. The over-expression of the TaRFS4B gene can improve the resistance of wheat to gibberellic disease and banded sclerotial blight at the same time, provides an effective technical means for prevention and control of the two diseases in production, further accelerates the breeding process of wheat disease resistance, perfects the prevention and control measures of the diseases, and has a wide application prospect.

The invention discloses a gibberellic disease resistance related receptor protein kinase gene as well as an expression vector and application thereof, and belongs to the field of gene engineering. The cDNA (complementary deoxyribonucleic acid) sequence of the TaRLK3-3B is SEQ ID NO. 1, and the amino acid sequence coded by the TaRLK3-3B is SEQ ID NO. 2. The gene is inserted into an expression vector pAHC25 to obtain an overexpression vector of the gene, and the overexpression vector of the gene is introduced into an infected wheat variety, so that the resistance of the wheat variety infected with the gibberellic disease to the gibberellic disease can be remarkably improved. The TaRLK3-3B is used for genetic engineering breeding, and when the TaRLK3-3B is introduced into wheat varieties susceptible to gibberellic disease, the gibberellic disease resistance of wheat can be improved.

The present application is suitable for the technical field of sorghum fusarium head blight identification, and provides a molecular marker for identifying sorghum resistance or susceptibility to fusarium head blight, comprising a molecular marker 6Sam72772 and / or Tx430508; the molecular marker 6Sam72772 can be a 126bp DNA fragment and a 122bp DNA fragment, the nucleotide sequence of the 126bp DNA fragment is shown as SEQ ID:5, and the nucleotide sequence of the 122bp DNA fragment is shown as SEQ ID:6; the molecular marker Tx430508 is a 720bp DNA fragment, and the nucleotide sequence is shown as SEQ ID:7. The present application also provides related primer groups, kits, and methods for identifying sorghum resistance or susceptibility to fusarium head blight. The present application provides the molecular markers 6Sam72772 and Tx430508, which can accurately identify or assist in identifying sorghum resistance or susceptibility to fusarium head blight.

A method for improving wheat resistance to Fusarium head blight (FHB) by genome editing is provided, and further provided is use of TaCIPK14 protein or related biomaterial thereto in the regulation of plant resistance to FHB; the TaCIPK14 protein is TaCIPK14-4A protein (SEQ ID NO: 4), TaCIPK14-4B protein (SEQ ID NO: 5), and / or TaCIPK14-4D protein (SEQ ID NO: 6). TaCIPK14 knockout mutant plants are successfully obtained by simultaneously editing three alleles of TaCIPK14 in wheat using CRISPR-Cas9 gene editing technology. The TaCIPK14 knockout mutant shows resistance to inoculation with FHB pathogens, and the results of evaluation of major agronomic traits confirm that the TaCIPK14 knockout mutant reserves the major agronomic traits.

This invention provides a method for breeding disease-resistant wheat varieties. [Solution] The wheat receptor-like protein kinase TaMLR1 provided by this application has a specific amino acid sequence, and the open reading frame (ORF) sequence encoding the wheat receptor-like protein kinase TaMLR1 is described in a specific nucleic acid sequence. In this disclosure, gene editing is performed on wild-type material Fielder to obtain TaMLR1 gene-edited mutant plants exhibiting resistance to wheat yellow rust, powdery mildew, and Fusarium head blight. This disclosure provides a novel technical approach for breeding disease-resistant varieties, confirms the negative regulatory role of this gene in wheat disease resistance, and provides a novel genetic resource for broad-spectrum disease-resistant breeding of wheat.

This invention discloses a 1-(2-aminobenzyl)-3-arylurea compound, its preparation method, and its uses. This type of compound is synthesized from o-aminobenzylamine and aryl isocyanate compounds as raw materials, using triethylamine as an acid-binding agent and dichloromethane as a solvent via a condensation reaction in an ice-water bath. The raw materials used in the synthesis of this type of compound are inexpensive and readily available, and the synthesis method is simple. It exhibits good inhibitory effects on crop pathogens, particularly showing significant inhibitory activity against Sclerotinia sclerotinia, Phytophthora blight, Fusarium head blight, Carbendazim, Botrytis cinerea, and Sheath blight.

The invention discloses a molecular design breeding method for rapidly improving wheat scab and powdery mildew resistance based on multi-gene polymerization, which comprises the following steps: taking a scab-resistant material NMAS084 which carries a plurality of scab-resistant genes and has excellent comprehensive agronomic characters as a recurrent parent, taking Yangmai 158 near-isogenic line Y7A2033 containing a powdery mildew-resistant gene Mlm2033 as a receptor parent, and carrying out multi-gene polymerization on the basis of the recurrent parent; a linkage marker M1 of a molecular marker powdery mildew resistance gene Mlm2033, a linkage marker M2 of Fhb1, a linkage marker M3 and M4 of Fhb2, a linkage marker M5 of Fhb4 and a linkage marker M6 of Fhb5 are used for auxiliary selection, and a gibberellic disease expansion resistance identification technology is combined, so that a new wheat germplasm with good adaptability, excellent comprehensive characters and resistance to gibberellic disease and powdery mildew is created; the method is of great significance to cultivation of wheat germplasm with wide spectrum-resistant range, stable and lasting disease resistance and excellent comprehensive characters.

This invention discloses a molecular breeding method, molecular markers, and primers for selecting varieties highly resistant to Fusarium head blight. The molecular markers include those with flanking sequences as shown in SEQ ID NO.3. TaFhb‑2DL The KASP marker. This invention utilizes fine-mapping populations to narrow down Fusarium head blight resistance sites. QFhb-2DL By analyzing the physical regions of the genome, candidate genes were identified, and the genes most associated with Fusarium head blight resistance were screened using the phenotypes and genotypes of natural populations. A new linkage marker, KASP.TaFhb-2DL, was developed to enable... QFhb-2DL The utilization is made more efficient. This invention utilizes genes carrying resistance to Fusarium head blight. TaFhb‑ 2DL Varieties with disease-resistant genotypes, used as parents, carry... Fhb1 Gene varieties serve as donors, because TaFhb‑2DL It has been effectively utilized in wheat scab resistance breeding in the middle and lower reaches of the Yangtze River, and on this basis, a major scab resistance gene was introduced. Fhb1 It can enhance resistance to Fusarium head blight to "high resistance," surpassing that of Sumai 3. This is achieved through the use of effective molecular markers. TaFhb‑2DL and Fhb1 The aggregation of these technologies can quickly and effectively breed varieties highly resistant to Fusarium head blight.

This invention discloses molecular markers, primers, and their applications closely linked to the major quantitative trait locus for wheat resistance to Fusarium head blight (Fusarium head blight) Qfhb.jaas-7DS, belonging to the field of agricultural biotechnology. This invention identifies a novel, stable major quantitative trait locus (QTL) for Fusarium head blight resistance on the short arm of chromosome 7D, named Qfhb.jaas-7DS. To effectively utilize this resistance locus, two KASP markers, KASP-109827800 and KASP-109891380, closely linked to this locus, were designed and developed based on the 7D chromosome genome sequence and SNP sequence information. These two markers serve as molecular markers for the Fusarium head blight resistance gene on the short arm of wheat chromosome 7D, and can be used for high-throughput detection of this locus, as well as assisted selection of Fusarium head blight resistant wheat varieties carrying this gene, thereby accelerating the breeding process of disease-resistant varieties.

The present invention relates to a methylene ester bridge-containing compound, and a preparation method therefor and the use thereof as a bactericide. The compound has a structure as shown in formula (I). The compound of the present invention has a broad-spectrum bactericidal activity against a variety of plant fungal diseases, can be used alone or in combination with other agents for the control of a variety of plant fungal diseases, and specifically has excellent control effects on rice sheath blight, cucumber gray mold, wheat Fusarium head blight and Sclerotinia stem rot in oilseed rape.

This invention discloses a TaSMT-5B gene for enhancing wheat resistance to Fusarium head blight and its applications. The TaSMT-5B gene belongs to the selenocysteine ​​methyltransferase family, catalyzing the methylation of SeCys to MeSeCys. It is stably and highly expressed in the high-MeSeCys-rich and Fusarium head blight-resistant variety Zheng 9023, and a "GTCGCCG" insertion site exists in the promoter region of this gene in the Zheng 9023 variety. The TaSMT-5B gene was overexpressed using a constructed overexpression vector and introduced into the popular variety Fielder via Agrobacterium infection, resulting in five stable transgenic lines. After basal application of inorganic selenium, the severity of Fusarium head blight in the transgenic plants was alleviated. This invention has significant production implications for achieving selenium biofortification in wheat and enhancing its resistance to Fusarium head blight, providing a new gene resource for breeding stable-yielding and high-quality wheat varieties.

The invention discloses a molecular marker for high-throughput identification of a wheat scab site Qfhb7EL and application of the molecular marker. The nucleotide sequences of primers of the molecular marker are as shown in SEQ ID No. 1 to SEQ ID No. 5. A primer is designed by using a specific sequence M7EL-13 on a diploid agropyron elongatum 7EL chromosome, the primer and an Actin gene of wheat form a primer pair, population typing is realized under recognition of different fluorescence, and the reliability of the marker is verified through a molecular experiment. Results show that the identification of the wheat scab-resistant site Qfhb7EL in the wheat background can be quickly and accurately realized in a high-throughput manner, and the method has a very high application value for auxiliary selection of new wheat scab-resistant varieties.

The invention discloses a multiple PCR (Polymerase Chain Reaction) Panel for detecting favorable allelic variation of a wheat gibberellic disease resistant site and application of the multiple PCR Panel. The Panel takes a gibberellic disease resistant major gene Fhb1 and important resistance sites QFhb5A and QFhb2D derived from backbone parents in the middle and lower reaches of the Yangtze River as targets, can synchronously and accurately detect multi-site allelic variation in a single-tube reaction, and has the advantages of high throughput, low cost, simplicity and convenience in operation, high specificity and the like. The invention further establishes a wheat gibberellic disease-resistant molecular design breeding strategy based on the Panel, forms a cooperative selection mode of'precise parent matching + multi-site targeted detection + comprehensive character evaluation ', and realizes rapid polymerization and efficient fixation of multi-site favorable allelic variation. In a word, the multi-PCR Panel and a matched molecular breeding strategy of the multi-PCR Panel are favorable for improving the targeted selection efficiency and accuracy of fusarium head blight resistance pyramiding breeding of the wheat, and a feasible technical means is provided for cultivating new wheat germplasm with stable resistance and coordinated comprehensive characters.

This invention discloses a gene, TaLAC58, capable of enhancing wheat resistance to Fusarium head blight and its applications. The TaLAC58 gene belongs to the laccase family and is a gene specifically expressed in the resistant wheat variety Sumai 3. This gene was overexpressed using a constructed overexpression vector and introduced into the popular variety Fielder via Agrobacterium infection, resulting in stable transgenic plants. In the three transgenic lines obtained, resistance to Fusarium head blight was significantly enhanced. This invention has significant production implications for improving wheat resistance to Fusarium head blight and provides a new gene resource for breeding high-yielding, stable-yielding, and widely adaptable new wheat varieties.

The present invention relates to an asymmetric aromatic diselenide compound and the use thereof in the preparation of pesticides, particularly to the use thereof in the preparation of anti-plant virus agents and fungicides. The asymmetric aromatic diselenide compound of the present invention exhibits a good efficiency on tobacco mosaic virus (TMV) at a concentration of 500 mg / L, achieving an in-vivo inactivation efficiency of 71.6%, an in-vivo protective efficiency of 58.3%, and an in-vivo therapeutic efficiency of 60.1%. The asymmetric aromatic diselenide compound of the present invention exhibits good control effects on tomato early blight, wheat Fusarium head blight, rice blast, pepper Phytophthora blight, rapeseed Sclerotinia rot, cucumber gray mold, rice sheath blight, cucumber wilt, peanut brown spot, apple ring rot, wheat sharp eyespot, southern corn leaf blight, watermelon anthracnose, and rice bakanae disease at a concentration of 50 mg / L, and has a fungicidal activity of up to 92.3%.

The application discloses application of three endogenous plant peptides in prevention and treatment of wheat scab and foot rot. The application first finds that three endogenous Pep precursors (TaPROPEP) can be cut to produce endogenous plant peptides TaPep, the endogenous plant peptides can act as immunostimulants, cause plant immune response, reduce infection degree of wheat scab and foot rot, and significantly improve wheat resistance to the wheat scab and foot rot in a greenhouse and in a field.

This invention discloses an N-aryl(2-acylaminobenzoyl)aminoamide compound, its preparation method, and its uses. This compound is generated by reacting Boc-amino acids and aromatic amines in dichloromethane under the action of EDCI, DMAP, and triethylamine. The N-Boc-aminobenzoyl compound is then acid-hydrolyzed with trifluoroacetic acid to obtain an aminobenzoyl compound. Next, the aminobenzoyl compound is reacted with indomethacin anhydride in acetonitrile under the action of potassium carbonate to generate a diamide compound. Finally, the diamide compound is reacted with an acyl halide in the action of triethylamine to obtain the N-aryl(2-acylaminobenzoyl)aminoamide compound. The raw materials used in the synthesis are inexpensive and readily available, and the synthesis method is simple. This compound exhibits good inhibitory activity against crop pathogens, particularly against Sclerotinia sclerotinia, Phytophthora blight, Fusarium head blight, rice blast fungus, gray mold, sheath blight, and rot fungus.

The invention belongs to the field of maize disease resistance identification and gene mining, and particularly relates to a maize fusarium ear rot resistance phenotype accurate identification method for adaptive gene mining, which comprises the following steps: S1, setting a resistance gene positioning identification garden, including a preliminary positioning identification garden and a fine positioning identification garden, selecting a land parcel which is flat in terrain, uniform in fertility, perfect in irrigation and drainage facilities, free of shading and prone to diseases as an identification nursery; s2, selecting a positioning population, adopting an F23 character segregation population and the like as a preliminary positioning identification nursery, and adopting an F3 population, an RIL population, a BC population and an RNA-SEQ detection material as a fine positioning identification nursery; and S3, performing field test design of the identification nursery. According to the method, accurate identification of the corn fusarium ear rot resistance phenotype is achieved by standardizing identification garden setting, optimizing inoculation and investigation processes and linking a molecular technology, reliable phenotype data support is provided for efficient positioning, cloning and related research of anti-disease genes, and corn anti-disease molecular breeding is assisted.