22 results about "Phomopsis" patented technology

The present invention discloses a strain of Trichoderma wumengense GUWM22 and its application, which belong to the field of microorganisms. The deposit number of the Trichoderma wumengense GUWM22 is CCTCC NO: M20242639, the deposit date is November 26, 2024, the deposit unit is the China Center for Type Culture Collection, and the deposit address is Wuhan University, Wuhan, China. It has been verified that the GUWM22 bacteria have a strong inhibitory effect on 6 pathogens (Phytophthora nicotianae, Phytophthora capsici, Botrytis cinerea, Alternaria alternata, Phomopsis simiana and Echinops planifolia), and the inhibition rate is more than 78%. In addition, GUWM22 also has a good prevention and control effect on post-harvest anthracnose of pepper (strip pepper), with a prevention effect of 82.11%. It can be seen that Trichoderma wumengense GUWM22 is a new species of biocontrol Trichoderma with good potential. The present invention provides a new strain resource and an environmentally friendly and efficient biocontrol approach for the effective prevention and control of pepper anthracnose.

The invention discloses application of fungus fermentation liquor to prevention and treatment of areca leaf spot disease. The invention relates to the technical field of agricultural biology, and provides application of fungus fermentation liquor in prevention and treatment of areca leaf spot disease. The fungus fermentation liquor is filtrate obtained by performing fermentation culture on specific fungi and filtering a culture solution to remove thalli; the specific fungus is phomopsis of RdRp protein from the PaFV1 virus through overexpression. The fungus fermentation liquor provided by the invention is sprayed on areca leaves, so that the occurrence of areca leaf spot disease can be obviously reduced, and good disease control potential is shown. The invention has important significance for preventing and treating areca leaf spot disease.

The invention discloses a salinity regulation and control seedling culture method for hibiscus syriacus, and belongs to the technical field of hibiscus syriacus seedling culture. The method comprises the following steps: (1) seed treatment; (2) preparing a substrate; (3) after sowing, culturing in a greenhouse; (4) transplanting and salinity control: a) when the seedlings grow to have 3-4 true leaves, transplanting the seedlings into a nutrition bag, adding 0.4-0.6 wt% of a microbial liquid into the matrix at the same time, the microbial liquid containing nitrogen-fixing bacteria Azobacter chrocococcus and phosphate-solubilizing bacteria Bacillus licheniformis, and the total number of viable bacteria being greater than or equal to 1 * 10 < 9 > CFU / mL; after transplanting, carrying out drip irrigation with brackish seawater, and spraying a 0.1% monopotassium phosphate solution on leaf surfaces every half a month for three times in total; b) directly carrying out drip irrigation on brackish seawater in the nutrition bag; and (5) outdoor cold-resistant treatment: 30 days before winter, spraying 0.2-0.5 L of 0.2-0.4 wt% potassium sulfate aqueous solution to leaf surfaces for each plant, spraying once every 6-8 days, and spraying 3-5 times in total. Through salinity gradient domestication and use of the microbial agent, the biomass loss rate of the hibiscus syriacus under the salinity of 0.5% is remarkably reduced, and the annual increment of the plant height is improved.

The present invention discloses a Brevibacillus strain, a bacterial agent and their applications and a preparation method of the bacterial agent. The strain is preserved in the China Center for Type Culture Collection (CCTCC), named Brevibacillus sp. BSB-3, and the preservation number is CCTCC NO: M 2025263. The maximum tolerance concentration of the strain to inorganic phosphorus reaches 2000 mg / L. In a liquid medium, after 7 days of culture, the removal rate of soluble phosphorus can reach more than 65.91%, and the accumulation amount of polyphosphate granules in the bacterial cells is significantly higher than that of conventional polyphosphate-accumulating bacteria. In addition, the strain can maintain stable polyphosphorus activity within the ranges of pH 5.0-8.0, temperature 20-35 °C and different carbon source and nitrogen source systems, and is particularly suitable for the treatment of high-phosphorus wastewater and the restoration of black and odorous water bodies.

The invention discloses a recombinant genetically engineered bacterium for producing a micafungin precursor FR901379 and an application of the recombinant genetically engineered bacterium. The recombinant genetically engineered bacterium for producing the micafungin precursor FR901379 is obtained by performing overexpression on an epigenetic modification factor in a phomopsis sheathing genome and performing screening to obtain the recombinant genetically engineered bacterium for producing the micafungin precursor FR901379. The epigenetic modification factor comprises a histone methyltransferase (Dot 1), a histone methyltransferase (Set2) or a histone deacetylase (Rpd3). The yield of FR901379 produced by the engineering strain is increased by 40% compared with that of an original strain. The method disclosed by the invention has the characteristics of simplicity and convenience in operation, high transformation efficiency and good genetic stability.

The invention relates to a quinoline hydrazone compound containing a hydrazone structure as well as a preparation method and application of the quinoline hydrazone compound, and relates to the technical field of chemical engineering and pesticides. According to the invention, 5-aminoquinoline is used as a raw material to synthesize a series of quinoline hydrazone compounds containing a hydrazone structure; the prepared compound can effectively prevent and treat plant fungal diseases including cucumber gray mold, potato late blight, sclerotinia rot of colza, tomato gray mold, wheat scab, rice blast, kiwi fruit phomopsis and rice sheath blight disease.

The invention provides a disease-resistant and growth-promoting aspergillus alabamate AF-Q5, a microbial preparation and application of the microbial preparation, and belongs to the technical field of microorganisms. The invention provides aspergillus alabamaensis AF-Q5, and the preservation number of the aspergillus alabamaensis AF-Q5 is CGMCC (China General Microbiological Culture Collection Center) No. 41805. The aspergillus alabamaensis AF-Q5 can be used for producing cellulase and has the capability of decomposing cellulose; the bactericidal composition has the inhibiting ability on pathogenic bacteria such as fusarium oxysporum, Adiplomonas minuta, fusarium oxysporum, phomopsis and phytophthora, can be applied to prevention and treatment of rice seedling blight and / or tomato fusarium wilt, can also promote growth of rice and / or tomatoes, and is beneficial to increase of the yield of rice and / or tomatoes and the like.

The present invention discloses the use of a fungal fermentation liquid for preventing and controlling betel nut leaf spot disease. This invention relates to the field of agricultural biotechnology and provides the use of a fungal fermentation liquid for preventing and controlling betel nut leaf spot disease. The fungal fermentation liquid is a filtrate obtained by fermenting and culturing a specific fungus, followed by filtering the culture liquid to remove the fungus cells. The specific fungus is Phomopsis raphis that overexpresses the RdRp protein derived from the PaFV1 virus. Spraying the fungal fermentation liquid provided by the present invention on betel nut leaves can significantly reduce the incidence of betel nut leaf spot disease, demonstrating excellent disease control potential. The present invention is of great significance for preventing and controlling betel nut leaf spot disease.

The invention relates to a denitrifying phosphorus-accumulating bacterium with a plant growth promoting function as well as a culture method and application of the denitrifying phosphorus-accumulating bacterium. The strain is (Pseudomonas sp.) DWP1; the preservation unit is CGMCC (China General Microbiological Culture Collection Center); the preservation number is CGMCC (China General Microbiological Culture Collection Center The strain has the advantages that the strain is high in growth speed and wide in strain carbon source utilization range, and the removal rate of NO3 <->-N in most carbon sources which can be utilized by the strain reaches 95% or above; according to the present invention, the rapid propagation of the strain is achieved under the outdoor condition, the optimal nutrient concentration of KNO3 is 1.9 g / L, the optimal nutrient concentration of sodium citrate is 2.7 g / L, and the optimal nutrient concentration of KH2PO4 is 0.58 g / L, only trace elements are required to be added, aeration is not required, the OD600 of the bacterial liquid of the strain under the concentration within 36 h can achieve 0.6, the nitrate nitrogen removal rate can achieve 98%, the TOC removal rate can achieve 100%, and the sulfate radical removal rate can achieve 66%; the strain can also be used as a plant growth-promoting bacterium to promote the growth of an aquatic plant duckweed and cooperate with plants to jointly purify a water body.

The invention provides an anti-soybean phomopsis seed rot related SNP molecular marker and application thereof, the SNP molecular marker is located at the 8462025th site of the No.18 chromosome of soybean, and the polymorphism is C / G. According to the invention, the SNP molecular marker associated with soybean phomopsis seed rot resistance is found, a target resource library of technicians in the field is enriched, and a new choice is provided for molecular marker-assisted breeding of soybeans. By detecting the SNP molecular marker, soybeans can be identified in the early stage of breeding, germplasm resources with high disease resistance can be quickly determined, and breeding and development of new soybean varieties with resistance to phomopsis are accelerated.

The invention belongs to the technical field of detection of pathogenic fungi, provides an LAMP (loop-mediated isothermal amplification) detection method of phomopsis cacao, and establishes a rapid, sensitive, convenient and visual loop-mediated isothermal amplification technology detection method of phomopsis cacao. Results show that the detection method disclosed by the invention is short in reaction time, strong in specificity, high in sensitivity, simple to operate and visual in detection result, is suitable for port quarantine and field detection of the phomopsis cacao, and provides technical support for rapid detection and precise prevention and control of foreign pests at ports and protection of ecological safety in China.

The invention provides a kiwi fruit fresh-keeping biocontrol bacterium and application thereof, the bacterial strain is classified and named as Bacillus amyloliquefaciens BacXF14, the preservation number of the bacterial strain is CGMCC (China General Microbiological Culture Collection Center) NO: 35691, and the preservation date is August 20, 2025. The bacterial strain has bacteriostatic activity on botryosphaeria dothidea, alternaria alternata, phomopsis, phomopsis vitis, botrytis cinerea and pestalotiopsis. The strain can effectively inhibit the growth of various pathogenic bacteria after harvesting of kiwi fruits, and the occurrence of soft rot caused by botryosphaeria dothidea infection is remarkably reduced. The composition of microbial community structures on the surfaces of the kiwi fruits can be regulated and controlled, the relative abundance of spoilage pathogenic bacteria is reduced, and the relative abundance of beneficial microorganisms is increased, so that the quality of the kiwi fruits is improved, and the preservation period of the fruits is prolonged. In conclusion, the strain has wide application potential and value in the postharvest preservation process of the kiwi fruits.

The present application relates to a kind of small and medium-sized river water bottom system ecological restoration method, including bottom mud matrix improvement and module construction and submerged plant planting, wherein bottom mud matrix improvement and module construction include: stripping bottom mud→laying geotextile→burying air pipe→preprocessing bottom mud backfilling→planting mycorrhizal plants and harvesting→throwing into polyphosphorus bacteria→laying stone cage net and stone cage net box.The present application includes the improvement of bottom mud matrix, river bottom module construction, underwater forest module planting and so on multiple aspects, combines river bottom mud matrix improvement and submerged plant planting two aspects closely, complement each other, promote each other, fundamentally solves river water ecological pollution problem using biological measures and engineering measures.

The invention discloses a cedarane ketone hydroxy-oxalaxyl ester derivative as well as a preparation method and application thereof, and belongs to the technical field of agricultural chemistry. The structure of the derivative is shown as a formula I, wherein R is selected from groups shown as E1-E13. According to the invention, safe and non-toxic cedarane ketone is used as a raw material, and oximation and esterification reactions are carried out to prepare a target derivative. The series of derivatives show remarkable and broad-spectrum inhibitory activity on various plant pathogenic fungi, especially phomopsis kiwifruit, valsa mali, verticillium dahliae, mucor and paecilomyces variotii, and the activity of the compound E8 is most prominent. The derivative disclosed by the invention can be used as an active ingredient for preparing a pharmaceutical composition for preventing and treating fungal diseases of plants, and a new choice is provided for developing efficient, low-toxicity and environment-friendly botanical pesticides.

The invention provides a method for screening soybean phomopsis psammophila response genes based on transcriptome analysis, which comprises the following steps: selecting pods of soybean plants infected with phomopsis psammophila and healthy plants planted in a field, carrying out transcriptome sequencing, screening differential expression genes responding to phomopsis psammophila in soybeans, and carrying out transcriptome sequencing on the screened differential expression genes to obtain the phomopsis psammophila response genes. The method comprises the following steps: analyzing a biological pathway of differential expression genes participating in a phomopsis stem rot infection process, carrying out protein interaction analysis, screening out interaction core genes, comparing sequences, carrying out transcript de novo assembly, and carrying out integration analysis on a difference variable shear event and an exon use difference, so as to obtain a sequence of the phomopsis stem rot. And analyzing the dynamic characteristics of the regulatory network after the transcription of the key candidate gene of the soybean responding to the phomopsis, and screening out the response gene of the soybean phomopsis. The four response genes of the soybean phomopsis are obtained, the expression characteristics of the four response genes in different soybean tissues are analyzed, and a theoretical basis is provided for resistance breeding of the soybean phomopsis.

The application provides a naringin derivative, a preparation method of the naringin derivative, and application of the naringin derivative in preparation of medicines for preventing and treating wheat scab, tobacco black stem disease, phomopsis stem rot and pepper wilt, and has good bacteriostatic activity on wheat scab fungus, tobacco black stem fungus, phomopsis stem rot fungus and pepper wilt fungus, has a good prevention and treatment effect on plant pathogenic fungal diseases such as wheat scab, tobacco black stem disease, phomopsis stem rot and pepper wilt, and has a broad application prospect and development potential.

The invention belongs to the technical field of water environment treatment, and relates to an in-situ remediation process method for rural black and odorous water, which is suitable for ecological treatment of black and odorous water bottom mud and low-temperature regions. The technology comprises the following steps: screening thiobacillus denitrificans, denitrifying bacteria and phosphorus-accumulating bacteria from the bottom mud of a target water area, preparing a special fungicide combination for three stages of deodorization, denitrification and phosphorus accumulation, and carrying out cross antagonism culture; drying-free agglomeration and wrapping granulation are adopted to prepare double-layer particles; injecting into a bottom mud anaerobic layer through a bottom mud injection device in three stages, and adding corresponding microbial agents according to the stages. Through cold-resistant flora directional reinforcement, double-layer particle controlled release and staged precise addition, the microbial inoculum activity and bottom mud permeability in a low-temperature environment are remarkably improved, the problems of microbial inoculum loss, agglomeration and insufficient pertinence are effectively solved, and efficient ecological management of black and odorous water is realized.

The invention belongs to the technical field of high-salinity aquaculture tail water treatment, and particularly provides a multi-trophic-level ecological purification system for high-salinity aquaculture tail water. Aiming at the current situations that the existing mariculture tail water is high in nitrogen and phosphorus content and high in salinity and the existing ecological treatment system is poor in purification effect, the invention provides a composite purification path with three synergistic systems of a spartina alterniflora system (nitrifying bacteria), a sea horse tooth (phosphorus-accumulating bacteria) and an ulva lactuca (algae symbiosis). According to the invention, light energy is absorbed, harmful algae are inhibited, suspended solids are physically intercepted and adsorbed, nitrogen, phosphorus, COD and heavy metal Cd in a water body environment are removed through cooperation of phosphorus-accumulating bacteria and denitrifying bacteria, the cost is reduced, and the economic income of by-products is increased.

The application discloses a kind of composite functional microbial inoculant load clay mineral / submerged plant-based biological carbon sequestration materials and preparation method, by certain proportion submerged plant Vallisneria, Ceratophyllum, Potamogeton, clay mineral sepiolite powder, green detritus powder, kaolin powder, aerobic bacteria yeast, lactic acid bacteria, photosynthetic bacteria, denitrifying bacteria, anaerobic bacteria Bacillus, nitrifying bacteria, phosphorus-fixing bacteria, pseudomonas are constituted, steps are as follows: (1) submerged plant biochar precursor preparation;(2) clay mineral modification treatment;(3) carrier preparation;(4) bacteria culture;(5) microbial inoculant load: under natural conditions, microbial inoculant load, after natural air drying, obtain the material;It also relates to the use of the material, promote microbial activity, improve microbial diversity, effectively improve the bottom condition, provide favorable growth environment for the recovery and reconstruction of submerged plant, significantly promote the growth of submerged plant, further increase the carbon input of submerged plant, enhance the ecological carbon sink function of lake ecosystem.

The application discloses a polyphosphorus subatlantic bacillus and application thereof, the polyphosphorus subatlantic bacillus 1-29 is identified as subatlantic bacillus ( Atlantibacter sp. ), is preserved in China Microbial Culture Collection Center (CGMCC), and the preservation number is CGMCC No.37506, and the preservation date is January 22, 2026.The polyphosphorus bacteria subatlantic bacillus 1-29 ( Atlantibacter sp. 1-29 ) provided in the application has a better phosphorus removal effect under the conditions that pH is 6-9, initial phosphorus concentration is below 4 mg / L and temperature is 15-30 DEG C, is above 85%;has a higher phosphorus removal potential in actual aquaculture water in-situ purification; and the strain has high safety, and has good application value in aquaculture tail water treatment.

The invention relates to the technical field of water treatment, in particular to a three-dimensional ecological synergistic method for repairing and treating eutrophic water, which mainly comprises the following steps: constructing constructed wetlands on two sides of a water inlet river channel and a water head shoal by utilizing hygrophytes and emergent aquatic plants; submerged plants and floating plants are planted in the shallow and sunny water areas; putting filtering algae, omnivorous fishes and carnivorous fishes into the water body according to the ecological niche; snail and mussel benthic mollusks are put into sludge at the bottom of the water body; splashing a complex microbial inoculant containing nitrifying bacteria, phosphorus-accumulating bacteria and lactic acid bacteria into the water body; the fishes are caught out of the water body, and hygrophytes, emergent aquatic plants, submerged plants and floating plants are removed out of the water body in a manual harvesting mode. By forming a complete substance flow closed loop of'exogenous interception-endogenous conversion-biological removal ', eutrophication substances such as nitrogen and phosphorus in the eutrophication water body can be efficiently and quickly reduced, and the economic benefits of the water body are improved by fully utilizing the eutrophication substances.

The application discloses a new molecular target specific to Burkholderia gladioli and a coconut poison pathogenic variety thereof and a rapid detection method thereof, and belongs to the technical field of molecular biology and microbial detection. A nucleotide sequence combination as a detected target in the application for identifying Burkholderia gladioli and a coconut poison pathogenic variety thereof is shown as SEQ ID NO. 1-3. The application can detect Burkholderia gladioli without physiological and biochemical identification, can make up for the defects that biochemical tests cannot identify the coconut poison pathogenic variety of high-toxicity Burkholderia gladioli, has short detection time, low cost, simple operation and strong specificity; the application can make up for the defects that existing molecular detection and molecular typing of Burkholderia gladioli have poor specificity and lack of methods for the coconut poison pathogenic variety of high-toxicity Burkholderia gladioli, the detection result is more accurate, the result determination is simpler, and the practicability is stronger.