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Application of tenuazonic acid in preparation of microbial source fungicide

A kind of Alternaria ketoacid, microbial source technology, applied in the direction of fungicides, biocides, chemicals for biological control, etc., can solve the problem that no one has reported the inhibitory effect of plant pathogens

Inactive Publication Date: 2015-07-15
ANHUI AGRICULTURAL UNIVERSITY
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  • Claims
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Problems solved by technology

However, no one has reported its inhibitory effect on plant pathogens

Method used

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  • Application of tenuazonic acid in preparation of microbial source fungicide
  • Application of tenuazonic acid in preparation of microbial source fungicide
  • Application of tenuazonic acid in preparation of microbial source fungicide

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Embodiment Construction

[0029] 1. Extraction and separation of antibacterial active substances:

[0030] 1) The endophytic fungus XXB07 of meadowsweet was fermented and cultured in a shaker at 28°C and 160r / min for 5 days, and the fermentation broth was filtered to obtain fermentation filtrate;

[0031] 2) Extract the fermentation filtrate three times with n-butanol, combine the n-butanol phases, and concentrate under reduced pressure at 60°C to obtain n-butanol extract;

[0032] 3) The n-butanol extracts were collected through two silica gel column layers, thin layer chromatography and HPLC to collect the fractions with antibacterial activity;

[0033] 4) The above-mentioned antibacterial active fractions are combined, and the high pressure preparation liquid phase collects the antibacterial active substances.

[0034] 2. Antibacterial test of antibacterial active substances:

[0035] 1) Cultivation of plant pathogenic microorganisms

[0036] Pick a small amount of slant cultures of apple rot bac...

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Abstract

The invention discloses application of tenuazonic acid in preparation of microbial source fungicide. The tenuazonic acid has a structural formula as described in the specification and a molecular formula of C10H15NO3 with a molecular weight of 197. The tenuazonic acid has inhibition activity to phytopathogens. According to identification results and biological activity experiment results, the tenuazonic acid has substantial bacteriostatic activity to Valsa mali, Phomopsis vexan and Venturia nashicola. When the tenuazonic acid with a concentration of 100 [mu]g / mL is applied, the inhibition rates of Valsa mali, Phomopsis vexan and Venturia nashicola are 95.9%, 77.6% and 100.0%, respectively; thus, it is proved that the tenuazonic acid has potential in development of the microbial source fungicide.

Description

technical field [0001] The invention belongs to the field of microbial pesticides, and in particular relates to antibacterial active substances isolated from endophytic fungus fermentation metabolites of Spiraea and its application prospects. Background technique [0002] The development of biological source pesticides with microbial metabolites or active substances isolated from microorganisms is one of the research hotspots and directions of new pesticide creation. For example, avermectin with insecticidal activity, Jinggangmycin with bactericidal activity and bialaphos (Bialaphos, produced by Streptomyces) with herbicidal activity, etc. [0003] Tenuazonic acid was first introduced in 1957 by Rosett et al. Alternaria tenuis It was isolated from the culture filtrate and its structure was determined by Stickings in 1959. Thereafter, in A. longipes, A. linicola, A. brassicicola, A. Raphani Aspergillus, sp., Pyricularia oryzae , Phoma sorghina Some people have isolated ...

Claims

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Application Information

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IPC IPC(8): A01N43/36A01P3/00
Inventor 花日茂吕培付稳吴祥为史陶中李湘琼柏钰马鑫操海群
Owner ANHUI AGRICULTURAL UNIVERSITY
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