200 results about "Streptomyces" patented technology

Disclosed is a novel Streptomyces sporosporus strain SC263 produced by UV mutagenesis, the produced antibiotics exhibit increased thermal stability, which exhibit antibiotic activity against bacteria and fungi. Antibiotics produced by SC263 and compositions thereof are also provided for the treatment of bacterial and fungal infections. The antibiotics have a very high molecular weight, which is suitable for topical administration.

The invention discloses preparation of a trichoderma composite flora and application of the trichoderma composite flora in prevention and control of crop fusarium wilt and improvement of soil, and relates to the field of agricultural production, and the composite flora is prepared by mixing trichoderma asperellum (5 * 10 < 7 > CFU / L), streptomyces (108 CFU / L), Chinese monospora (108 CFU / L), ensifer adhesion (108 CFU / L), pseudomonas nitroreducens (108 CFU / L) and paenibacillus alvei (108 CFU / L) according to the volume ratio of 6: 1: 1: 1: 1: 1: 1: 1 (V: V). According to the composite flora in the scheme, the composite flora is used for promoting growth of cucumber plants, and compared with a clear water control group (Foc), the fresh weight of cucumbers is increased by 55.51%, the stem diameter is increased by 12.76%, and the plant height is increased by 34.25%. The composite flora can also effectively prevent and treat fusarium wilt of cucumbers, and the prevention and treatment effect reaches 71.43%. Compared with the Trichoderma asperellum FJ035 treated group, the Trichoderma asperellum FJ035 has the advantages that the Trichoderma asperellum FJ035 is better than the Trichoderma asperellum FJ035 which is independently applied in the aspects of promoting the growth of cucumber plants and preventing and treating cucumber fusarium wilt, and compared with the Trichoderma asperellum FJ035 treated group, the control effect is improved by 56.25%. Meanwhile, the composite flora can be used for improving soil and preventing and treating multi-crop fusarium wilt.

The invention discloses talaromyces purpurogenum as well as a fermented supernatant and application thereof. The preservation number of the talaromyces purpureogenus F-4 is CCTCC NO: M 20252023, and the talaromyces purpureogenus F-4 has the growth promoting effects of dissolving phosphorus, fixing nitrogen and generating siderophores, also has the disease preventing and treating effects of preventing and treating garlic root rot, potato scab and wheat stem rot, and also can resist salt stress, alkali stress, salt-alkali composite stress, heavy metal stress and osmotic pressure stress. The fermentation supernatant of the talaromyces purpureus F-4 can be used for inhibiting the growth of fusarium solani spores, streptomyces botrytis spores and fusarium pseudograminearum and the germination of the spores of the fusarium solani, the streptomyces botrytis and the fusarium pseudograminearum. The talaromyces purpurogenus and the fermented supernatant thereof can effectively control the growth and reproduction of plant pathogens, reduce the risk of disease occurrence, reduce the dependence on chemical pesticides, and reduce the risk of environmental pollution and agricultural product residues, and have important significance for promoting the sustainable development of agriculture.

The invention belongs to the technical field of microorganisms, and discloses a soil microorganism for promoting the growth of tea trees and improving the quality of tea leaves, the strain name is Streptomyces cerevisiae SI-3B1, the preservation unit is Guangdong Microbial Culture Collection Center, the preservation date is September 13, 2024, and the preservation number is GDMCC No.65129. The invention further discloses a preparation method of the soil microorganism for promoting the growth of the tea trees and improving the quality of the tea leaves. The microorganisms effectively improve the content of theanine and water extract of fresh leaves of one bud and two leaves of large-leaf spring tea, summer tea leaves are thicker, and the biomass of the leaves is increased.

The invention relates to the technical field of microorganisms, in particular to streptomyces noursei and application of the streptomyces noursei in preparation of polymycotin B. The invention provides streptomyces noursei GBC A-20 which is preserved in the general microbiological center of the China Committee for Culture Collection of Microorganisms, and the preservation number of the streptomyces noursei GBC A-20 is CGMCC No.27277. The strain can be used for efficiently fermenting and producing the multi-fungal element B by utilizing cheap raw materials, the yield of the multi-fungal element B is remarkably improved, and the chemical titer and the biological titer of the fermentation product nysfungin are greatly improved; the strain and the application thereof provide an economical and feasible solution for the industrial production of the multi-fungal element B and the nysfungin.

The invention discloses streptomyces griseorubens and an application of the streptomyces griseorubens in phthalate pollution remediation. The bacterial strain is Streptomyces griseorubens FZ202, the preservation number of the bacterial strain is GDMCC No: 61372, the bacterial strain has good environmental adaptability and efficient degradation capacity, DEHP can be effectively degraded within 100-800 mg / L, the highest degradation efficiency can reach 94.1%, and complete degradation can be achieved. The strain is inoculated into PAEs contaminated soil and interacts with rice, DEHP residues in the soil and crop bodies can be effectively reduced, various PAEs can be synergistically degraded, and the strain has the broad-spectrum degradation characteristic. Meanwhile, through multiple mechanisms of degrading pollutants, promoting plant growth, improving the soil environment and regulating and controlling microbial communities, the strain remarkably improves the growth performance of rice and the self-cleaning capacity of soil, and has a wide agricultural application prospect.

The invention discloses a growth-promoting rhizosphere bacterium streptomyces cerevisiae QS9-7 and application thereof, and belongs to the technical field of microorganisms. According to the invention, a strain of Streptomyces setonii QS9-7 is obtained through separation, and the preservation number of the Streptomyces setonii QS9-7 is CGMCC (China General Microbiological Culture Collection Center) No.32329. Experiments show that the strain QS9-7 has a relatively strong inhibition effect on various plant pathogens, and shows excellent biocontrol bacterium potential. The strain can effectively improve radicle length, germ length, lateral root number and germination rate of plant seeds and plant height and stem diameter of plant seedlings, obviously promotes plant growth, and has high application value in green prevention and control of plant diseases.

The invention discloses a special microbial fertilizer for cotton and a preparation method thereof, and belongs to the technical field of microbial fertilizers. The fertilizer is prepared from the following raw materials in parts by weight: 40-60 parts of a microbial agent, 80-100 parts of a microbial agent carrier, 40-60 parts of a nitrogen fertilizer, 30-50 parts of a phosphate fertilizer, 20-30 parts of a potassium fertilizer and 10-20 parts of a stabilizer, the microbial agent is prepared from nitrogen-fixing paenibacillus mucilaginosus MSSW01, bacillus nissi and streptomyces widermomole, and the microbial agent is prepared from bacillus mucilaginosus MSSW01 and streptomyces widermomole. The stabilizer is prepared from roxburgh rose extract, soybean protein isolate and gamma-aminobutyric acid according to the mass ratio of 2: 1: (0.2-0.5). By screening and optimizing three beneficial microbial strains and combining the synergistic effect of raw material components in a specific ratio, the yield and quality improvement of cotton can be remarkably promoted, and meanwhile, the occurrence rate of common diseases of cotton is effectively reduced.

The invention provides a plasmid, an alpha-ketoglutaric acid engineering strain and a construction method and application of the alpha-ketoglutaric acid engineering strain, the strain is obtained by adopting a directional transformation method, transcriptional levels of katE, katG, sodB and grxA genes are up-regulated, a catR gene and a katA gene are heterologously expressed, a high-copy plasmid pETKG is carried, and the plasmid simultaneously expresses L-glutamate oxidase LGOX genes from Streptomyces sp and Chain A; the strain self-synthesized catalase eliminates H2O2 generated in the process of producing alpha-ketoglutaric acid through enzyme catalysis, engineering bacteria of L-glutamate oxidase LGOX expressed by pETKG plasmids are used for performing whole-cell catalysis on L-sodium glutamate to synthesize alpha-ketoglutaric acid, and the engineering strain is a high-level engineering strain capable of industrially and stably producing alpha-ketoglutaric acid.

The invention relates to the technical field of agricultural biology, and discloses a microbial agent for preventing and treating fructus aurantii scab and a preparation method of the microbial agent. The microbial agent comprises the following raw materials in percentage by weight: 10-13% of paenibacillus mucilaginosus; 15%-20% of trichoderma sp. 7%-10% of paecilomyces lilacinus; 5%-8% of bacillus pumilus; 7%-9% of mycorrhizal fungi; 5%-8% of streptomyces; 5%-9% of a coptis chinensis extract; 4%-7% of a sophora flavescens extract; 2%-4% of realgar; 1%-3% of a rheum officinale extract; 10%-13% of soybean meal; 5%-8% of a water-soluble fertilizer; 7%-9% of potassium fulvate; 1%-3% of chitosan; 0.5%-1% of trace elements; 3%-8% of humic acid; the raw materials are subjected to microbial flora amplification culture, plant antibacterial component light-shielding mixing and low-temperature mixing to effectively protect the microbial activity of the microbial agent, and step-by-step operation can avoid damage of high temperature and mechanical shearing to microorganisms, so that the microbial activity of the microbial agent is effectively protected. And the loss of microbial active ingredients is reduced by vacuum drying and low-temperature granulation processes.

The invention discloses a collagenase mutant, a gene segment, a recombinant plasmid, a recombinant expression system and application thereof, and a method for preparing the collagenase mutant and collagen tripeptide, and belongs to the field of gene engineering and fermentation engineering. The invention provides a collagenase mutant KT231 capable of realizing high-efficiency secretory expression in streptomyces S.lividans TK24 and a preparation method of the collagenase mutant KT231. The collagenase mutant KT231 can be used for efficiently carrying out enzymolysis on the skin of the narrow gadus at 60 DEG C, has excellent heat resistance and higher enzyme activity, and can be used for efficiently preparing the collagen tripeptide from the skin of the narrow gadus. The collagen peptide is compounded with commercially available papaya chymotrypsin, so that the proportion of collagen tripeptide in the fish skin enzymatic hydrolysate can be remarkably increased, and the fish skin enzymatic hydrolysate has no bitter taste. The method has wide application prospects in preparation of products, tissue engineering materials and wound healing accelerators for resisting wrinkles, preserving moisture, repairing damaged skin and improving bone and joint health.

The invention discloses a compound microbial organic fertilizer and a preparation method thereof, and belongs to the technical field of compound microbial organic fertilizers. The compound microbial organic fertilizer is prepared from the following raw materials in parts by weight: 8-12 parts of a microbial agent, 30-35 parts of livestock and poultry manure, 20-25 parts of crop straws, 10-15 parts of humic acid, 10-12 parts of bagasse carbon, 3-5 parts of rumen bypass fat powder, 3-5 parts of molasses and 1-3 parts of chitosan oligosaccharide. According to the compound microbial organic fertilizer, the nitrogen-fixing pseudomonas, the streptomyces elongisporus and the paenibacillus polymyxa are selected to be compounded, and the three strains comprehensively promote root development, nutrient absorption and stress resistance of strawberry plants through the synergistic effect of nitrogen fixation, nutrient activation, hormone secretion and disease inhibition. The compound microbial organic fertilizer can effectively promote growth of strawberry plants, improve the quality of strawberries, increase the yield, reduce chemical fertilizer and pesticide residues, reduce the risk of soil pollution and meet the requirements of consumers for safe and organic strawberries.

The present invention relates to a composition comprising components (A) and (B) as active ingredients, wherein component (A) comprises a Streptomyces and component (B) is a compound selected from the group consisting of sulphur, phosphonate, copper hydroxide, copper oxychloride, bicarbonate, Bacillus sp, Trichoderma sp, aureobasidin A, jawsamycin, kasugamycin, natamycin, a polysaccharide elicitor, a plant biostimulant (PBS) comprising an algal extract, a plant extract and / or vinasse, orange oil, and / or tea tree oil. The invention further relates to a method of controlling or preventing phytopathogenic diseases, preferably phytopathogenic fungi, on a plant or on plant propagation material and / or on harvested food crops, which comprises applying to the plant, on plant propagation material, the locus thereof, and / or on harvested food crops the composition according to the present invention.

The invention discloses a directed evolution strategy of type II sesquiterpene cyclase (SsDMS) derived from Streptomyces shedoensis based on alanine scanning, and an application of the type II sesquiterpene cyclase (SsDMS) derived from Streptomyces shedoensis based on alanine scanning. The invention relates to a plurality of SsDMS key mutant sites capable of catalytically generating a novel drive type sesquiterpenoid compound, which comprise substitution of sites 208, 248, 249, 497 and 505, and the SsDMS key mutant sites comprise substitution of sites 208, 248, 249, 497 and 505. The invention discloses an efficient escherichia coli cell factory applied to heterologous expression of an SsDMS mutant and efficient production of a novel drive type sesquiterpene compound, and further obtains the drive type sesquiterpene compound with a novel structure. According to the present invention, the efficient production of the drive type sesquiterpene compound by using the SsDMS and the mutant thereof is achieved, the modification strategy has wide applicability, the important enzymology resource is provided for the biosynthesis of the novel terpene compound, and the more choices are provided for the discovery of the drug lead compound.

The invention provides a dynamic control system for coordinating streptomyces secondary metabolite production and application of the dynamic control system. Firstly, a general artificial promoter PQS (trigger) responding to a quorum sensing system of various streptomycetes is designed, then a stabilizer is constructed based on a bistable gene circuit, instantaneous signal output is converted into stable signal output, dependence of an inducer is successfully eliminated, finally, an orthogonal amplifier is developed based on a streptomycete rare extracellular function sigma factor, and finally, the specific function of the quorum sensing system of various streptomycetes is achieved. And different promoter element libraries containing amplifiers with response from weak to strong and with strength span of about 535 times are constructed. An amplifier and a CRISPRi system are combined to construct a turner, and gene expression inhibition with the strength span of about 200 times is achieved. The combination of the amplifier, the promoter library and the turner is called a multi-effect device, and abundant elements are provided for fine target gene expression. The combination of the trigger, the stabilizer and the multiple-effect device is called as a streptomycete multipath manual control system (SMARTS), and control tools of streptomycete are enriched.

The invention belongs to the field of leavening agents, and particularly relates to a compound microbial agent for cow dung compost fermentation and a preparation method and application thereof. The compound microbial agent is a mixed bacterial agent of bacillus subtilis (HBU33), streptomyces althiophosphaticus (HBU11) and aspergillus niger (Aspergillus niger) HBUN19, and the compound microbial agent is a mixed bacterial agent of bacillus subtilis (HBU33), streptomyces althiophosphaticus (HBU11) and Aspergillus niger (HBUN19). The compound microbial agent is applied to cow dung compost, and can effectively shorten the fermentation time of cow dung compost, improve the compost temperature, promote organic matter degradation and lignocellulose decomposition, accelerate the composting process of fermented compost and reduce the loss of nutrient substances, thereby ensuring the stability of the compost quality.

The invention discloses a microbial complex microbial inoculant, a preparation method thereof and application of the microbial complex microbial inoculant in red mud treatment, and relates to the technical field of mine ecological restoration. The invention provides a composite microbial inoculant which comprises a microbial inoculant microorganism A, a microbial inoculant microorganism B and a microbial inoculant microorganism C in a weight ratio of (1-3): (1-4): 1, the microbial inoculum microorganism A is at least one of rhizobium, azotobacter, pseudomonas, bacillus, burkholderia and glomus; the microbial inoculum microorganism A is at least one of rhizobium, azotobacter, pseudomonas, bacillus, burkholderia and glomus; the microbial agent microorganism B is at least one of desulfurization vibrio, streptococcus, acetobacter and acidophilic thiobacillus; the microbial agent microorganism C is at least one of agrobacterium, enterobacter, streptomyces and klebsiella. The microbial agent disclosed by the invention can effectively solve the problems of high salt and alkali content of the red mud and heavy metal toxicity, and also can create conditions suitable for plant growth on the red mud, so that the ecological restoration effect is improved.

The invention discloses a microbial agent for increasing manure compost temperature and application thereof in high-temperature compost, and belongs to the field of agricultural solid waste treatment. The microbial agent for increasing the manure composting temperature comprises phanerochaete chrysosporium, bacillus stearothermophilus, penicillium oxalicum, streptomyces, common thermoactinomycetes and bacillus subtilis. The livestock padding manure high-temperature clean composting technology is established through adjustment and optimization, the purposes that the temperature of the padding manure is increased fast, the high-temperature period is long, the composting period is shortened, base materials are fully degraded are achieved, reference can be provided for resource utilization of livestock and poultry breeding solid waste, and important application value is achieved.

The invention belongs to the technical field of genetic engineering, and particularly relates to a method for increasing the yield of lincomycin by knocking out a streptomyces lincomycin pAgene0296 gene. The method comprises the following steps: knocking out a coding gene pAgene0296 of TetR / AcrR family repressor protein in streptomyces lincomycin, and fermenting the obtained mutant strain to produce lincomycin. Experimental results show that after the pAgene0296 gene of the streptomyces lincomycin is knocked out, the yield of the lincomycin is remarkably increased. The invention further provides a construction method of the pAgene0296 gene deletion type streptomyces lincoli mutant strain, according to the method, the coding gene of the streptomyces lincoli is directionally modified through a gene engineering technology, so that the pAgene0296 gene is knocked out, and the obtained pAgene0296 gene deletion type streptomyces lincoli mutant strain can be used for efficiently producing lincomycin or a product in the synthesis process of the lincomycin.

The invention discloses a polyketone compound derived from actinomycetes as well as a preparation method and application thereof, and relates to the technical field of microbial natural product mining and biological medicine, and the key point of the technical scheme is that the invention discloses a novel polyketone compound Strepactone derived from actinomycetes Streptomyces sp. DP0001, and the molecular formula of the novel polyketone compound Strepactone is C25H38O5. The compound is obtained through strain fermentation, ethyl acetate extraction and multi-step chromatographic purification, and the structure is identified through ESI-MS, 1H NMR and 13C NMR. Experiments show that Strepactone has an inhibition effect on staphylococcus aureus and methicillin-resistant staphylococcus aureus and has remarkable inhibition activity on human non-small cell lung cancer A549 cells, the half inhibitory concentration IC50 of the Strepactone is 5.36 mu M, and the Strepactone has no obvious cytotoxic activity on human embryo kidney cells 293T cells at the concentration of 30 mu M. The compound provided by the invention can be used for preparing anti-drug-resistant bacteria drugs and anti-human non-small cell lung cancer drugs, and also provides a structural basis for the development of novel antibacterial and anti-tumor leading drugs.

The invention discloses a strain of streptomyces angustifolius and application of the streptomyces angustifolius. The streptomyces angusticus disclosed by the invention is separated from banana rhizosphere soil, is named as a strain GD7-29, is preserved in the Guangdong Microbial Culture Collection Center on June 26, 2024, and has the preservation number of GDMCC No: 64803. The streptomyces angusticus disclosed by the invention has the advantages that the streptomyces angusticus is named as a strain GD7-29, and the preservation number is GDMCC No: 64803. The streptomyces narrow-mycin strain GD7-29 can effectively inhibit various plant pathogenic fungi including fusarium oxysporum, can promote plant growth, can be used for preventing and treating plant pathogenic fungus diseases, and particularly can be applied to preventing and treating banana fusarium wilt and promoting banana growth. The streptomyces narrow-mycin strain GD7-29 disclosed by the invention has a good application prospect in the aspects of preventing and treating plant fungal diseases and promoting plant growth.

The invention provides a nitrification inhibitor for promoting root hair growth, a biostimulant and a preparation method and application of the biostimulant, the nitrification inhibitor comprises 3, 4-dimethylpyrazole and streptomyces fermentation liquor with the weight ratio of 1: (2.5-4.0), and the streptomyces fermentation liquor contains organic acid. The nitrification inhibitor provided by the embodiment of the invention can effectively promote the growth of root hair, further, the biostimulant prepared from the nitrification inhibitor can effectively induce up-regulation expression of auxin related genes ZmARF10, ZmYUC2, ZmYUC3, ZmYUC5, ZmPIN1 and ZmPIN9, the growth of crop root hair is promoted, the root activity of plants is greatly improved, the high-quality growth and yield of crops are promoted, and meanwhile, the biostimulant has good application prospects. The raw materials of the biostimulant are easy to obtain, the preparation method is simple, and industrialization is easy to realize.

The invention relates to the technical field of compound microbial additives, and particularly discloses a compound microbial additive suitable for alpine regions and a preparation method, the additive comprises microbial thalli, a protective agent and a carrier; the microorganisms are low-temperature-resistant nitrogen-fixing bacteria, cold-resistant phosphate-solubilizing bacteria, cold-resistant potassium-solubilizing bacteria, low-temperature-resistant plant growth-promoting rhizobacteria and cold-resistant streptomyces; the selected microbial strains are all derived from extreme environments of the alpine regions, are naturally selected and artificially domesticated for a long time, and have extremely strong adaptability to severe conditions such as low temperature and oxygen deficit of the alpine regions. Compared with the existing microbial additive in the market, the growth rate and metabolic activity of the microbial strain in the additive are kept at a relatively high level in a low-temperature environment of 0-15 DEG C.

The invention relates to the technical field of agricultural microorganisms, and provides a microbial agent for promoting growth of fruits and vegetables, the microbial agent is mainly prepared from a compound bacteria liquid, the total viable count of the compound bacteria liquid is greater than or equal to 1 * 10 < 10 > CFU / g, and the compound bacteria liquid comprises the following components in percentage by viable bacteria: 35-40% of aspergillus terreus, 10-20% of aspergillus niger, 5-10% of aspergillus niger, 5-10% of aspergillus niger, and the balance of water. 10 to 20% of bacillus subtilis; 15 to 20% of streptomyces sp. 10-12% of saccharomyces cerevisiae; and 18 to 25% of pseudomonas chlororaphis. The invention overcomes the defects of the prior art, is reasonable in design and relatively comprehensive in function, and also solves the technical problems that the function of the existing single microbial agent is limited and the activity of the complex microbial agent is relatively low under a high-temperature condition.

The present invention discloses a compound microbial agent for alleviating continuous cropping obstacles of traditional Chinese medicine crops and its application. The compound microbial agent of the present invention contains Bacillus amyloliquefaciens Y34, Serratia marcescens Y35, Bacillus amyloliquefaciens Y40 and Streptomyces SD; it can be applied to improve soil, eliminate diseases of Polygonatum odoratum, Pogostemon cablin and Cynanchum stauntonii, and promote crop growth. The compound microbial agent of the present invention can reduce soil-borne diseases during the planting process of traditional Chinese medicine, alleviate continuous cropping obstacles of traditional Chinese medicine, improve the yield and quality of traditional Chinese medicine, and has important application value especially in the cultivation and production of traditional Chinese medicine crops such as Polygonatum odoratum and Pogostemon cablin.

The invention belongs to the technical field of microbial pharmacy, and discloses a polyketone macrolide compound generated by genetically engineered streptomyces as well as a preparation method and application thereof. The polyketone macrolide compound disclosed by the invention is a cinnamyl streptomycin derivative, has cell proliferation inhibition activity better than that of a natural product Cinnamomycin A-D, can exert anti-tumor activity by inhibiting activity of human exonucleotide pyrophosphatase / phosphodiesterase 1 (ENPP1) and activating an inherent immune pathway, can be prepared into a medicine or a medicinal composition, and can be used for preparing medicines or medicinal compositions. The compound is used for treating tumors and related diseases.

The invention discloses a biosynthesis method and application of cannabinoid compounds. The specific method comprises the following steps: constructing a non-reductive polyketide synthase (NRPKS) AtnG from a fungus Arthrinum sp.NF2194, an isopentenyl transferase NphBV49W / Y288P from an actinomycetes Streptomyces sp.CL190, a cyclase Svz9 from an actinomycetes Streptoviensis NA431, a GPP synthase AgGPPS from a plant Abis grandis and a terpene synthesis enhancement path MEV into a heterologous expression vector, and sequentially introducing the heterologous expression vector into escherichia coli, so as to successfully realize the production of the cannabinoid compounds OA, CBGA and CBCA. The cannabinoid compound disclosed by the invention has important medical and industrial values.

The invention provides a fermentation method of clavulanic acid, and relates to the technical field of biological pharmacy. The method comprises the following steps: inoculating streptomyces clavuliformis into a shake flask seed culture medium, and carrying out shake flask seed culture; inoculating the cultured shake flask seeds to a seed culture medium, and performing seed tank culture; the method comprises the following steps: inoculating a strain into a fermentation culture medium, culturing in a fermentation tank, centrifugally separating the obtained fermentation liquid, and separating and purifying the supernatant liquid to obtain clavulanic acid; wherein in the 20th hour of fermentation, glycerol is supplemented; supplementing the mixed supplementary material at the 40th hour of fermentation; the mixed supplementary material comprises L-glutamic acid, magnesium chloride, dipotassium phosphate, glacial acetic acid and potassium hydroxide. According to the method disclosed by the invention, the glycerol and the mixed supplementary material are supplemented at a specific time point in the fermentation process of the clavulanic acid, so that the fine regulation and control of the fermentation process are realized, and the technical effect of remarkably improving the titer of the clavulanic acid is achieved.

The invention discloses a preparation method and application of a novel heterocyclic isomer sourced from streptomyces. The heterocyclic isomer is separated from a fermentation culture of streptomyces and has a remarkable antibacterial effect.

The invention discloses a naphthocycline type pentacyclic aromatic polyketone compound. The structural formula of the naphthocycline type pentacyclic aromatic polyketone compound is as shown in a formula (I): # imgabs0 #. According to the invention, six naphthocycline type pentacyclic aromatic polyketone compounds are separated from a rice fermentation product of actinomycetes Streptomyces sp.NAK5495 for the first time, and the six naphthocycline type pentacyclic aromatic polyketone compounds are separated from rice fermentation products of actinomycetes Streptomyces sp.NAK5495; according to the present invention, with the medium-pressure liquid chromatography (MPLC) system and the semi-preparative HPLC separation and purification technology, the efficient separation and purification of the naphthocycline pentacyclic aromatic polyketones are achieved, and the recovery rate and the purity of the target compound are improved.