49 results about "Streptomyces" patented technology

The invention discloses a polyketone compound derived from actinomycetes as well as a preparation method and application thereof, and relates to the technical field of microbial natural product mining and biological medicine, and the key point of the technical scheme is that the invention discloses a novel polyketone compound Strepactone derived from actinomycetes Streptomyces sp. DP0001, and the molecular formula of the novel polyketone compound Strepactone is C25H38O5. The compound is obtained through strain fermentation, ethyl acetate extraction and multi-step chromatographic purification, and the structure is identified through ESI-MS, 1H NMR and 13C NMR. Experiments show that Strepactone has an inhibition effect on staphylococcus aureus and methicillin-resistant staphylococcus aureus and has remarkable inhibition activity on human non-small cell lung cancer A549 cells, the half inhibitory concentration IC50 of the Strepactone is 5.36 mu M, and the Strepactone has no obvious cytotoxic activity on human embryo kidney cells 293T cells at the concentration of 30 mu M. The compound provided by the invention can be used for preparing anti-drug-resistant bacteria drugs and anti-human non-small cell lung cancer drugs, and also provides a structural basis for the development of novel antibacterial and anti-tumor leading drugs.

The invention belongs to the technical field of microbial pharmacy, and discloses a polyketone macrolide compound generated by genetically engineered streptomyces as well as a preparation method and application thereof. The polyketone macrolide compound disclosed by the invention is a cinnamyl streptomycin derivative, has cell proliferation inhibition activity better than that of a natural product Cinnamomycin A-D, can exert anti-tumor activity by inhibiting activity of human exonucleotide pyrophosphatase / phosphodiesterase 1 (ENPP1) and activating an inherent immune pathway, can be prepared into a medicine or a medicinal composition, and can be used for preparing medicines or medicinal compositions. The compound is used for treating tumors and related diseases.

This invention discloses a strain of Streptomyces gravidarum and its application, belonging to the field of biotechnology. The preservation number of the Streptomyces gravidarum is: CCTCC NO: M 20251912. The Streptomyces gravidarum can be used to control rice sheath blight.

The application discloses a kind of for preventing and treating cruciferous crops root rot complex microbial inoculant, comprising: the rose chain-forming Streptomyces agent numbered B98035, the Trichoderma citrinoviride agent numbered BMZ102895 and the Bacillus sonorensis agent numbered BMZ065932;The volume ratio of the rose chain-forming Streptomyces agent, Trichoderma citrinoviride agent and Bacillus sonorensis agent is 1:2-4:2, wherein the effective viable count in the rose chain-forming Streptomyces agent, Trichoderma citrinoviride agent is not less than 1×10 8 CFU / ml, and the effective viable count in Bacillus sonorensis agent is not less than 1×10 9 CFU / ml.The complex microbial inoculant of the application can effectively prevent and treat cruciferous crops root rot caused by Plamodiophora brassicae.

The application discloses application of eugenol in preparation of a bacteriostatic agent for enhancing symbiotic nitrogen fixation efficiency of legume-rhizobium, and relates to the field of agricultural microorganisms. ‑1 The application is safe and non-inhibiting to seed germination of Medicago polymorpha and target rhizobium LL2 in a concentration range of 500-1000 muL / L ‑1 of eugenol, can significantly reshape rhizosphere microbial community structure by selectively inhibiting competitive microorganisms such as bacillus and streptomyces in soil, and is environment-friendly and free of antibiotic residue risk, thereby providing a green and efficient new approach for improving symbiotic nitrogen fixation efficiency of legume-rhizobium.

The present application relates to the technical field of biological medicine, in particular to streptomyces AH1901-2 and application thereof, preparation method and application of naphthymycin compounds, and belongs to the field of biological medicine. The streptomyces AH1901-2 is isolated from rhizosphere soil of Ligularia sibirica in Tianshan region (5.8°E, 28.0°N) in Xinjiang, and naphthymycin compounds are isolated and purified from liquid fermentation liquor of the streptomyces AH1901-2, and subsequent studies show that the naphthymycin compounds have obvious inhibitory effect on NO production of mouse mononuclear macrophages, and can be used for preparing anti-inflammatory drugs and treating inflammation.

The invention belongs to the field of biological pharmacy, and particularly relates to a chalcanthicidin compound and application thereof. The invention discloses a chalcanthicidin compound which is separated from a secondary metabolite of marine actinomycetes Streptomyces sp. KCB-132 (marine actinomycetes sp. KCB-132). Pharmacological activity experiments show that the compound has a moderate inhibition effect on ESKAPE pathogenic bacteria with multiple drug resistance, such as enterococcus faecium, staphylococcus aureus, klebsiella pneumoniae, acinetobacter baumannii and escherichia coli. Therefore, the compound has the potential of being developed into a novel antibacterial agent.

The invention belongs to the technical field of antiviral drugs for livestock and poultry, and discloses streptomyces LM-35, a metabolite of the streptomyces LM-35 and application of the streptomyces LM-35 in preparation of drugs for treating or preventing porcine reproductive and respiratory syndrome virus infection. A fermentation supernatant of the streptomyces LM-35 is used for carrying out anti-infection research on PRRSV at a cell level and an animal body level, and the streptomyces LM-35 is found to have remarkable anti-PRRSV activity in vivo and in vitro. The survival rate of the pigs fed with the streptomyces-containing LM-35 fermentation liquor reaches 80%; the adverse effect of PRRSV infection on the body can be effectively controlled, and the method can be used for preventing and controlling the PRRSV.

The application discloses a method for rapidly detecting geosmin-producing Streptomyces in Daqu samples, and relates to the technical field of liquor brewing microbial detection, and comprises the following steps: selectively culturing target Daqu samples to obtain Streptomyces enrichment culture solution; extracting nucleic acids from the Streptomyces enrichment culture solution and performing probe method fluorescence quantitative PCR amplification to obtain Streptomyces specificity fluorescence signal determination results; performing pretreatment to obtain a to-be-tested sample extract; performing quantitative analysis to obtain geosmin and its content data; and integrating the data to obtain the final determination results of geosmin-producing Streptomyces. The application combines the enrichment, probe method fluorescence quantitative PCR and GC-MS quantification, realizes integrated detection of Streptomyces identification and geosmin quantification in Daqu, and greatly improves the detection integrity and efficiency. The specific probe method qPCR combined with the optimized HS-SPME-GC-MS condition significantly improves the specificity and sensitivity of target bacteria and odor-causing substance detection, and effectively avoids false positives and matrix interference.

The invention discloses a methyltransferase mutant for efficient biocatalytic synthesis of S-adenosylmethionine and analogues thereof and a method for alkylation modification by using the methyltransferase mutant, and belongs to the technical field of bioengineering. According to the present invention, the optimal three mutants M11F / L30I / P209R are screened by using the wild type AteHMT as the template by using the directed evolution means, the S-adenosylmethionine (SAM) and the analogue thereof are synthesized by using the mutants through the biological catalysis, and the catalytic activity of the mutants is 1.5-23.5 times higher than the catalytic activity of the wild type AteHMT; the mutant, carbon methyltransferase CouO from streptomyces reesei and 4 '-O-methyltransferase (Mentha x piperea) FL-4 from mentha flavonoids are subjected to cascade reaction to form a series of alkylate products. The method has the advantages of mild conditions, environmental friendliness, high stereoselectivity, high yield and the like.

The application discloses a recombinant gluconacetobacter engineering strain with high vitamin B 12 yield and a construction method and application thereof, and belongs to the technical field of engineering strains. The construction method comprises the following steps: taking gluconacetobacter as a starting strain, and introducing a pos5 gene coding NADH kinase to construct a recombinant gluconacetobacter engineering strain with high vitamin B 12 yield. By heterologously expressing the NADH kinase (pos5) gene from saccharomyces cerevisiae, the transformation of NADH in the cytoplasm of the gluconacetobacter to NADPH is promoted, the content of NADPH is increased by 70%, thereby the metabolic flux is improved, more flows to the VB 12 synthesis pathway, finally the sugar conversion rate is increased by 31%, and the VB 12 yield is increased by 9%. The application provides new technologies and applications for subsequent improvement of VB 12 biosynthesis in the gluconacetobacter.

The invention belongs to the field of biological pharmacy, and discloses a chalcanthicidin compound and application thereof in preparation of antitumor drugs. The chalcanthicidin compound is separated from a secondary metabolite of marine actinomycetes Streptomyces sp. KCB-132, and the chalcanthicidin compound is named as Gricidin A. The chalcanthicidin compound has the advantages that the chalcanthicidin compound can be used for preparing the chalcanthicidin compound; an anti-tumor activity experiment discovers that the Gricidin A has a remarkable inhibition effect on three strains of non-small cell lung cancer cells (NCI-H460, NCI-H1975 and A549), and has the potential of being developed into the medicine for treating the non-small cell lung cancer.

This invention belongs to the field of bioengineering and relates to glutamate oxidase mutants and their application in the biotransformation of α-ketoglutarate. The glutamate oxidase is derived from *Streptomyces molybdenum*, and a series of mutants were obtained through saturation mutation of its 536th amino acid residue. Among them, mutant C536E exhibits significantly enhanced enzyme activity. After expression, induction, and purification, the specific enzyme activity of the mutant is 2.06 U / mg, which is approximately 56% higher than that of wild-type glutamate oxidase. This invention also provides a method for constructing the glutamate oxidase mutant and its application in the preparation of α-ketoglutarate. Compared with existing technologies, the mutants provided by this invention have higher catalytic efficiency and show good application potential in the biotransformation of α-ketoglutarate.

This invention discloses a *Streptomyces xylose-lyticus* strain P6-2-1 antagonistic to multiple plant pathogenic fungi and its applications. *Streptomyces xylose-lyticus* P6-2-1 was deposited on November 27, 2025, at the China General Microbiological Culture Collection Center (CGMCC) with accession number CGMCC No. 36794, and is classified as *Streptomyces xylose-lyticus*. Streptomyces xylanilyticus The *Streptomyces xylose-degrading* P6-2-1 strain is effective against *Alternaria alternata*, *Fusarium solanum*, *Fusarium oxysporum* race 1 (Cuba specialized type), *Fusarium oxysporum* race 4 (Cuba specialized type), and *Fusarium graminearum*. 、 Verticillium dahliae exhibits inhibitory effects and a broad spectrum of inhibition. It can be effectively used to control plant diseases including apple rot, apple leaf spot, apple replant disease, banana wilt, cotton wilt, and corn stem rot, showing promising prospects for biocontrol applications.

The invention discloses a biosynthetic gene cluster of polyether polydecalinmycin and an application of the biosynthetic gene cluster in construction of a high-yield strain. According to the invention, a biosynthetic gene cluster pdm of the polyether polydecalinmycin is positioned in a genome of streptomyces marincalensis SCSIO 03032, and a high-yield strain of the polydecalinmycin is successfully constructed by overexpressing a positive regulation gene pdmRI or pdmRII coded by the pdm gene cluster in a wild strain, so that the yield of the polydecalinmycin is increased by 48 times, and a material basis is provided for large-scale preparation, development and application of the polydecalinmycin.

The application belongs to the technical field of biological medicine, and discloses a preparation method and application of a bunge leaf oil microemulsion gel. The method is characterized in that: 5-O-beta-D-glucosyl shikonin is generated by fermentation of Streptomyces CGMCC 4.1001 to modify shikonin, and then the 5-O-beta-D-glucosyl shikonin is combined with eucalyptus oil, a composite surfactant (Tween 80 / Span 80 = 4:1), n-butanol and water to construct an O / W type microemulsion, then a xanthan gum / kaolin gum mixture is introduced and calcium ions are used to trigger in-situ crosslinking to form a nano-network microemulsion gel. The application successfully solves multiple technical contradictions such as poor water solubility of shikonin, easy evaporation of eucalyptus oil, poor compatibility of microemulsion and gel, etc. by using a three-in-one technical path of biotransformation modification-microemulsion construction-in-situ gelation, and realizes the unity of high drug loading, high stability, high transdermal and good use feeling in a single delivery system. The application is suitable for external treatment of skin inflammation, wound healing and itching relief with moderate pH and no irritation.

This invention discloses a glutamine transaminase mutant and its applications, belonging to the field of bioengineering technology. The glutamine transaminase mutant of this invention is obtained by mutation based on the wild-type glutamine transaminase shown in SEQ ID NO.1. This invention modifies the substrate spectrum of the glutamine transaminase derived from Streptomyces, and the obtained glutamine transaminase mutant can catalyze the synthesis of nicotinamide from nicotinic acid. Combined with the synthesis method of this invention, an extremely high nicotinic acid conversion rate can be achieved, significantly increasing the yield of the product nicotinamide. Therefore, the glutamine transaminase mutant of this invention has good prospects for industrial application in nicotinamide synthesis.

The present application relates to natamycin production technical field, specifically to a method for improving natamycin fermentation yield, comprising the following steps: shake flask seed culture: under sterile conditions, brown yellow spore streptomyces spores are coated on agar medium, and placed in a culture box for culture, then the cultured brown yellow spore streptomyces is prepared into spore suspension, and the spore suspension is taken and added into a triangular flask of seed culture medium for culture; fermentation tank fermentation culture: the cultured seed liquid is inoculated into a fermentation culture medium comprising proteose peptone, yeast powder, sodium chloride, magnesium sulfate, glucose, betaine, ampicillin, sodium succinate for stirring culture, and glucose is added during the culture until the fermentation is completed, and natamycin is obtained. The present application significantly improves the yield of brown yellow spore streptomyces producing natamycin by adding betaine, ampicillin and sodium succinate in the fermentation culture medium; the production method is simple to operate and is conducive to large-scale industrialization.

The present disclosure provides a method for culture of bacterial strains, comprising the following steps: inoculating a bacterial strain in a first culture medium comprising 0.1 mg / L-0.8 mg / L of 1-bromohexane to obtain a first culture. The method for culture of bacterial strains provided by the present disclosure further comprises: inoculating the first culture into a second culture medium, wherein the second culture medium comprises 0.2 mg / L-0.8 mg / L of 1-bromohexane. The method can be used for fermentation of Gluconacetobacter melanogenum, Propionibacterium, Corynebacterium, Brevibacterium, Bacillus, Gluconobacter, Lactobacillus, Agromyces, Actinobacillus, Escherichia coli, Anaerobiospirillum, Trichoderma, Streptomyces, Aspergillus, Saccharomyces or Myceliopthora, effectively preventing infection of bacteriophages during fermentation.

The invention discloses a compound microbial fertilizer and a preparation method thereof, and the compound microbial fertilizer is prepared from the following raw materials in parts by weight: 100-150 parts of a bacillus siamensis microbial agent, 150-200 parts of a streptomyces pourpuensis microbial agent, 130-175 parts of a synergist, 185-215 parts of an auxiliary material, 110-140 parts of a chelate material, 41-78 parts of a catalyst, 10-30 parts of an active agent, 1-3 parts of an inhibitor, 0.5-1.5 parts of a thickening agent and 100-150 parts of water. The preparation method of the compound microbial fertilizer is simple and easy to implement, all the materials are added according to a specific sequence, it can be guaranteed that the materials are fully dissolved and mixed, the stability and uniformity of the fertilizer are guaranteed, and industrial production is facilitated; by applying the compound microbial fertilizer disclosed by the invention, the problem of lack of trace elements of crops can be directly solved, the growth and development of the crops are comprehensively optimized through the synergistic interaction of amino acids, the goals of quality improvement, yield increase, disease resistance and environmental protection are achieved, and the compound microbial fertilizer conforms to the development requirements of green, high-end and efficient agriculture and has wide popularization and application prospects.

This invention discloses a process for the co-culture synthesis of ε-polylysine by Streptomyces and molds. Streptomyces IFO14147 is used as the ε-polylysine producing bacterium, and various other molds are used as inducing bacteria. Both are co-cultured under the same culture medium and fermentation conditions to synthesize ε-polylysine. During the co-culture process, the fermentation broth is immersed in cation exchange resin to control the concentration of ε-polylysine in the system. Utilizing the inhibitory effect of ε-polylysine on molds, an appropriate ε-polylysine concentration is selected to control mold growth. This allows the molds to continuously synthesize biological inducers that promote the synthesis of ε-polylysine by Streptomyces IFO14147, ultimately resulting in increased fermentation yield.

The invention relates to a strong promoter p75 suitable for streptomyces and application thereof. The strong promoter p75 suitable for streptomyces is as follows: (a) a nucleotide sequence as shown in SEQ ID NO.1; or (b) a nucleotide sequence which is obtained by substituting, deleting and / or adding one or more nucleotides to the nucleotide sequence as shown in SEQ ID NO.1 and has the function of the strong promoter; or (c) a nucleotide sequence which is hybridized with the sequence shown in SEQ ID NO.1 under a highly rigorous condition and has the function of the strong promoter, or (d) a nucleotide sequence which has 75% or more consistency with the nucleotide sequence in a), b) or c) and has the function of the strong promoter. The strong promoter p75 can efficiently start gene transcription in various mode streptomycetes, is suitable for gene transcription and expression of common streptomycete mode strains, and has important significance on expression of actinomycete source genes in streptomycetes and high yield of important metabolites.

The invention relates to the technical field of microorganisms, and particularly provides application of trichoderma asperellum MM7 in constructing a drought-resistant banana rhizosphere microflora and improving the drought resistance of bananas. The trichoderma asperellum MM7 inoculation can relieve the inhibition of drought stress on the growth of banana seedlings and improve the water content and survival rate of tissues; the root antioxidant enzyme activity is enhanced, the accumulation of osmotic regulation substances is promoted, and the membrane lipid peroxidation degree is reduced; the expression of ABA metabolism and signal channel related genes and aquaporin genes is up-regulated; a rhizosphere bacterial community structure under drought stress is remodeled, beneficial functional bacteria such as pseudomonas, streptomyces and nitrococcus are remarkably enriched, and the drought resisting function of the bacillus amyloliquefaciens depends on indigenous microbial communities. The invention reveals that the trichoderma asperellum MM7 provides a new strain resource for developing a banana drought-resistant biological agent based on microbiome regulation through a synergistic interaction mechanism of directly regulating host physiology and gene expression-indirectly remodeling rhizosphere beneficial microflora.

The invention discloses phenylalanine ammonia lyase and application of the phenylalanine ammonia lyase in preparation of S-type beta-amino acid. Compared with an amino acid sequence as shown in SEQ ID NO: 1, the amino acid sequence of the phenylalanine ammonia lyase comprises one or more amino acid residue differences in the following sites: the 147th site, the 244th site, the 251st site, the 314th site, the 333th site and the 342nd site. The S-type beta-amino acid is prepared by adopting a mutant of phenylalanine ammonia lyase from streptomyces and taking cinnamic acid or a derivative thereof as a substrate, and the regioselectivity, the enantiomer selectivity and the conversion rate of the product are all remarkably improved.

The invention belongs to the field of synthetic biology and microbial pharmacy, and particularly relates to recombinant streptomyces, a construction method of the recombinant streptomyces and application of the recombinant streptomyces in preparation of savermectin B. According to the present invention, after the aveA1 gene segment in the abamectin biosynthesis gene cluster in the streptomyces avermectin is replaced with the nemA1 gene segment in the nemadectin biosynthesis gene cluster in the streptomyces blue griseolus, the recombinant streptomyces capable of producing the 16-membered macrolide compound-Sesvermectin B (Senvermectin B), such as the recombinant streptomyces avermectin HU520-M provided by the invention, is obtained; experiments show that the savermectin B has efficient insecticidal, acaricidal and anti-parasitic activity, and shows good industrial development potential.

The invention belongs to the technical field of water treatment denitrification, and particularly relates to a strain-piezoelectric material synergistic denitrification system and application thereof. The strain-piezoelectric material synergistic denitrification system comprises streptomyces denitrifying bacteria and barium strontium titanate, wherein the barium strontium titanate is loaded on the surfaces of the streptomyces denitrifying bacteria. According to the method, barium strontium titanate generates a piezoelectric effect through mechanical supply, electron donors are provided for streptomyces denitrifying bacteria, and the electron transfer efficiency is improved, so that the denitrification efficiency is improved. Experiments show that under the optimal conditions of 0.5 g / L barium strontium titanate concentration and 400 r / min stirring speed, the removal rate of total nitrogen and nitrate nitrogen of the system is higher than that of independent nitrogen removal of the strain DD, and meanwhile, the removal rate of total organic carbon is also greatly improved.

The application discloses a preparation method of a microbial solid fumigation preparation and application of the microbial solid fumigation preparation in grey mould prevention and treatment. Fresh wheat kernels are washed, boiled until the kernels are opened, dried, and then weighed and put into a conical flask, and the bottom of the flask is shaken and flattened, and the wheat kernels are subjected to high-temperature sterilization treatment twice to obtain a wheat kernel culture medium; a streptomyces xiyangyangensis WY228 fermentation liquor; the streptomyces xiyangyangensis WY228 fermentation liquor is added into the wheat kernel culture medium, mixed thoroughly, and then cultured, and the flask is shaken twice a day during the culture, and after the culture is completed, the microbial solid fumigation preparation is obtained by drying and storing. The microbial solid fumigation preparation can significantly inhibit the growth of botrytis cinerea by producing bacteriostatic gas, does not introduce live bacteria and excess moisture on the surface of the treated agricultural products, has safe and efficient prevention and treatment capacity on the grey mould, and has a good market application prospect.

The invention relates to application of streptomyces mnorhizosphere and fermentation liquor thereof in inhibition of colletotrichum truncatum resisting picolinamide, and belongs to the technical field of biological prevention and control of crop diseases. The biocontrol strain 14-3 is named as streptomyces farnesii, is preserved in the Guangdong Microbial Culture Collection Center, and has the preservation number of GDMCC No. 63693. The strain shows remarkable antagonistic activity on colletotrichum truncatum with picolinamide resistance, and fermentation liquor of the strain can effectively inhibit mycelial growth of pathogenic bacteria, germination of conidia and formation of attachment spores and can cause spore splitting decomposition. In addition, fermentation liquor of the strain has a good prevention and treatment effect on soybean anthracnose caused by infection of colletotrichum truncatum resisting picolinamide, and shows good application potential in the aspect of crop drug resistance treatment.

The invention belongs to the technical field of genetically engineered bacteria, and particularly discloses apramycin genetically engineered bacteria with genetic stability as well as a construction method and application of the apramycin genetically engineered bacteria. The construction method comprises the following steps: by taking a plasmid containing an xylE gene and a tsnR gene as a template, obtaining a linearized vector skeleton without a promoter; the method comprises the following steps: inserting a streptomyces indicus promoter sequence into a linearized vector skeleton to enable the promoter to be located at the upstream of an xylE gene and a tsnR gene, and constructing a double-reporter gene expression vector; transferring the expression vector into streptomyces through conjugation to obtain a conjugate; the genetically stable strain is obtained through ultraviolet mutagenesis in combination with catechol chromogenic screening. The biological value is increased by more than 40% compared with that of an original strain, the value retention rate of 94.5% or above is kept in continuous 10 generations of long-term passage experiments, and a powerful technical support is provided for industrial stable production of anti-mycoplasma active substances.

Generally, some problems can be annotated. The invention discloses a composting treatment method of tobacco production waste, and belongs to the technical field of agricultural composting. The method comprises the following steps: drying, crushing and sieving the tobacco waste to obtain fine powder; 100 parts of the tobacco waste fine powder, 0.1-1 part of the composite microbial inoculum loaded by the modified microbial inoculum carrier, 5-8 parts of the nitrogen source composition and 25-40 parts of the carbon source regulator are mixed, the water content is regulated, fermentation and aging are performed, and the matured compost is obtained. The modified microbial agent carrier is formed by compounding modified starch, chitosan and nano-hydroxyapatite, and is loaded with a composite microbial agent consisting of bacillus subtilis, halotolerant bacillus, bacillus amyloliquefaciens, bacillus cereus, streptomyces thermophilus, saccharomyces cerevisiae and pseudomonas stutzeri. By optimizing the material ratio and the functional fungicide, lignocellulose degradation and nicotine decomposition are promoted, the initial C / N ratio is increased, nitrogen loss is reduced, the decomposition period is shortened, and high-quality decomposed compost is obtained.