111 results about "Streptomyces" patented technology

The invention discloses talaromyces purpurogenum as well as a fermented supernatant and application thereof. The preservation number of the talaromyces purpureogenus F-4 is CCTCC NO: M 20252023, and the talaromyces purpureogenus F-4 has the growth promoting effects of dissolving phosphorus, fixing nitrogen and generating siderophores, also has the disease preventing and treating effects of preventing and treating garlic root rot, potato scab and wheat stem rot, and also can resist salt stress, alkali stress, salt-alkali composite stress, heavy metal stress and osmotic pressure stress. The fermentation supernatant of the talaromyces purpureus F-4 can be used for inhibiting the growth of fusarium solani spores, streptomyces botrytis spores and fusarium pseudograminearum and the germination of the spores of the fusarium solani, the streptomyces botrytis and the fusarium pseudograminearum. The talaromyces purpurogenus and the fermented supernatant thereof can effectively control the growth and reproduction of plant pathogens, reduce the risk of disease occurrence, reduce the dependence on chemical pesticides, and reduce the risk of environmental pollution and agricultural product residues, and have important significance for promoting the sustainable development of agriculture.

The invention discloses streptomyces griseorubens and an application of the streptomyces griseorubens in phthalate pollution remediation. The bacterial strain is Streptomyces griseorubens FZ202, the preservation number of the bacterial strain is GDMCC No: 61372, the bacterial strain has good environmental adaptability and efficient degradation capacity, DEHP can be effectively degraded within 100-800 mg / L, the highest degradation efficiency can reach 94.1%, and complete degradation can be achieved. The strain is inoculated into PAEs contaminated soil and interacts with rice, DEHP residues in the soil and crop bodies can be effectively reduced, various PAEs can be synergistically degraded, and the strain has the broad-spectrum degradation characteristic. Meanwhile, through multiple mechanisms of degrading pollutants, promoting plant growth, improving the soil environment and regulating and controlling microbial communities, the strain remarkably improves the growth performance of rice and the self-cleaning capacity of soil, and has a wide agricultural application prospect.

The invention discloses a growth-promoting rhizosphere bacterium streptomyces cerevisiae QS9-7 and application thereof, and belongs to the technical field of microorganisms. According to the invention, a strain of Streptomyces setonii QS9-7 is obtained through separation, and the preservation number of the Streptomyces setonii QS9-7 is CGMCC (China General Microbiological Culture Collection Center) No.32329. Experiments show that the strain QS9-7 has a relatively strong inhibition effect on various plant pathogens, and shows excellent biocontrol bacterium potential. The strain can effectively improve radicle length, germ length, lateral root number and germination rate of plant seeds and plant height and stem diameter of plant seedlings, obviously promotes plant growth, and has high application value in green prevention and control of plant diseases.

The invention relates to the technical field of agricultural biology, and discloses a microbial agent for preventing and treating fructus aurantii scab and a preparation method of the microbial agent. The microbial agent comprises the following raw materials in percentage by weight: 10-13% of paenibacillus mucilaginosus; 15%-20% of trichoderma sp. 7%-10% of paecilomyces lilacinus; 5%-8% of bacillus pumilus; 7%-9% of mycorrhizal fungi; 5%-8% of streptomyces; 5%-9% of a coptis chinensis extract; 4%-7% of a sophora flavescens extract; 2%-4% of realgar; 1%-3% of a rheum officinale extract; 10%-13% of soybean meal; 5%-8% of a water-soluble fertilizer; 7%-9% of potassium fulvate; 1%-3% of chitosan; 0.5%-1% of trace elements; 3%-8% of humic acid; the raw materials are subjected to microbial flora amplification culture, plant antibacterial component light-shielding mixing and low-temperature mixing to effectively protect the microbial activity of the microbial agent, and step-by-step operation can avoid damage of high temperature and mechanical shearing to microorganisms, so that the microbial activity of the microbial agent is effectively protected. And the loss of microbial active ingredients is reduced by vacuum drying and low-temperature granulation processes.

The present invention relates to a composition comprising components (A) and (B) as active ingredients, wherein component (A) comprises a Streptomyces and component (B) is a compound selected from the group consisting of sulphur, phosphonate, copper hydroxide, copper oxychloride, bicarbonate, Bacillus sp, Trichoderma sp, aureobasidin A, jawsamycin, kasugamycin, natamycin, a polysaccharide elicitor, a plant biostimulant (PBS) comprising an algal extract, a plant extract and / or vinasse, orange oil, and / or tea tree oil. The invention further relates to a method of controlling or preventing phytopathogenic diseases, preferably phytopathogenic fungi, on a plant or on plant propagation material and / or on harvested food crops, which comprises applying to the plant, on plant propagation material, the locus thereof, and / or on harvested food crops the composition according to the present invention.

The invention discloses a polyketone compound derived from actinomycetes as well as a preparation method and application thereof, and relates to the technical field of microbial natural product mining and biological medicine, and the key point of the technical scheme is that the invention discloses a novel polyketone compound Strepactone derived from actinomycetes Streptomyces sp. DP0001, and the molecular formula of the novel polyketone compound Strepactone is C25H38O5. The compound is obtained through strain fermentation, ethyl acetate extraction and multi-step chromatographic purification, and the structure is identified through ESI-MS, 1H NMR and 13C NMR. Experiments show that Strepactone has an inhibition effect on staphylococcus aureus and methicillin-resistant staphylococcus aureus and has remarkable inhibition activity on human non-small cell lung cancer A549 cells, the half inhibitory concentration IC50 of the Strepactone is 5.36 mu M, and the Strepactone has no obvious cytotoxic activity on human embryo kidney cells 293T cells at the concentration of 30 mu M. The compound provided by the invention can be used for preparing anti-drug-resistant bacteria drugs and anti-human non-small cell lung cancer drugs, and also provides a structural basis for the development of novel antibacterial and anti-tumor leading drugs.

The invention relates to the technical field of agricultural microorganisms, and provides a microbial agent for promoting growth of fruits and vegetables, the microbial agent is mainly prepared from a compound bacteria liquid, the total viable count of the compound bacteria liquid is greater than or equal to 1 * 10 < 10 > CFU / g, and the compound bacteria liquid comprises the following components in percentage by viable bacteria: 35-40% of aspergillus terreus, 10-20% of aspergillus niger, 5-10% of aspergillus niger, 5-10% of aspergillus niger, and the balance of water. 10 to 20% of bacillus subtilis; 15 to 20% of streptomyces sp. 10-12% of saccharomyces cerevisiae; and 18 to 25% of pseudomonas chlororaphis. The invention overcomes the defects of the prior art, is reasonable in design and relatively comprehensive in function, and also solves the technical problems that the function of the existing single microbial agent is limited and the activity of the complex microbial agent is relatively low under a high-temperature condition.

The invention belongs to the technical field of microbial pharmacy, and discloses a polyketone macrolide compound generated by genetically engineered streptomyces as well as a preparation method and application thereof. The polyketone macrolide compound disclosed by the invention is a cinnamyl streptomycin derivative, has cell proliferation inhibition activity better than that of a natural product Cinnamomycin A-D, can exert anti-tumor activity by inhibiting activity of human exonucleotide pyrophosphatase / phosphodiesterase 1 (ENPP1) and activating an inherent immune pathway, can be prepared into a medicine or a medicinal composition, and can be used for preparing medicines or medicinal compositions. The compound is used for treating tumors and related diseases.

The invention discloses a biosynthesis method and application of cannabinoid compounds. The specific method comprises the following steps: constructing a non-reductive polyketide synthase (NRPKS) AtnG from a fungus Arthrinum sp.NF2194, an isopentenyl transferase NphBV49W / Y288P from an actinomycetes Streptomyces sp.CL190, a cyclase Svz9 from an actinomycetes Streptoviensis NA431, a GPP synthase AgGPPS from a plant Abis grandis and a terpene synthesis enhancement path MEV into a heterologous expression vector, and sequentially introducing the heterologous expression vector into escherichia coli, so as to successfully realize the production of the cannabinoid compounds OA, CBGA and CBCA. The cannabinoid compound disclosed by the invention has important medical and industrial values.

The invention discloses a preparation method and application of a novel heterocyclic isomer sourced from streptomyces. The heterocyclic isomer is separated from a fermentation culture of streptomyces and has a remarkable antibacterial effect.

This invention discloses a strain of Streptomyces gravidarum and its application, belonging to the field of biotechnology. The preservation number of the Streptomyces gravidarum is: CCTCC NO: M 20251912. The Streptomyces gravidarum can be used to control rice sheath blight.

The application discloses a kind of for preventing and treating cruciferous crops root rot complex microbial inoculant, comprising: the rose chain-forming Streptomyces agent numbered B98035, the Trichoderma citrinoviride agent numbered BMZ102895 and the Bacillus sonorensis agent numbered BMZ065932;The volume ratio of the rose chain-forming Streptomyces agent, Trichoderma citrinoviride agent and Bacillus sonorensis agent is 1:2-4:2, wherein the effective viable count in the rose chain-forming Streptomyces agent, Trichoderma citrinoviride agent is not less than 1×10 8 CFU / ml, and the effective viable count in Bacillus sonorensis agent is not less than 1×10 9 CFU / ml.The complex microbial inoculant of the application can effectively prevent and treat cruciferous crops root rot caused by Plamodiophora brassicae.

The invention belongs to the technical field of soil improvement, particularly relates to a compound microorganism saline-alkali soil conditioner as well as a preparation method and application thereof, and aims at solving the problems that the existing soil conditioner mostly adopts a single strain or a simple combination, is difficult to colonize in a harsh saline-alkali environment, has a single function, cannot systematically improve the physical and chemical properties of soil, is slow in organic material decomposition and is difficult to promote. In order to solve the problems that in the prior art, the soil conditioner is not durable in effect due to the fact that the soil conditioner is prone to failure due to saline-alkali stress, the following scheme is provided: the soil conditioner comprises the following raw materials in parts by weight: 5-10 parts of bacillus; by using bacillus, streptomyces, saccharomycetes, actinomycetes, nitrogen-fixing bacteria, phosphate bacteria, potassium bacteria, pyroligneous liquor, seaweed extract, amino acid chelate, potassium fulvate, citric acid and monopotassium phosphate, the content of organic matters in soil can be effectively increased, the aggregate structure of the soil is improved, a more lasting soil improvement effect is achieved, and the soil conditioner has a good application prospect. And the use frequency and cost of the modifier are reduced.

The invention discloses streptomyces-sourced acidic cellulase S4-Cel2 and application thereof, and belongs to the technical field of genetic engineering, the amino acid sequence of the acidic cellulase S4-Cel2 is as shown in SEQ ID NO.2, and the nucleotide sequence of a gene for coding the acidic cellulase S4-Cel2 is as shown in SEQ ID NO.1. The invention further discloses a preparation method of the acidic cellulase S4-Cel2. The optimal reaction pH of the acidic cellulase S4-Cel2 is 4.5, the optimal reaction temperature is 50 DEG C, the acidic cellulase S4-Cel2 keeps the relative enzyme activity of 62% or above in a buffer system at the temperature of 40-60 DEG C, and still keeps the relative activity of 20% or above after being treated at the temperature of 60-80 DEG C for 1 h, and the acidic cellulase S4-Cel2 can be applied to the fields of food, feed, textile, environmental protection, energy and industry to achieve efficient enzymolysis of cellulose.

The application discloses application of eugenol in preparation of a bacteriostatic agent for enhancing symbiotic nitrogen fixation efficiency of legume-rhizobium, and relates to the field of agricultural microorganisms. ‑1 The application is safe and non-inhibiting to seed germination of Medicago polymorpha and target rhizobium LL2 in a concentration range of 500-1000 muL / L ‑1 of eugenol, can significantly reshape rhizosphere microbial community structure by selectively inhibiting competitive microorganisms such as bacillus and streptomyces in soil, and is environment-friendly and free of antibiotic residue risk, thereby providing a green and efficient new approach for improving symbiotic nitrogen fixation efficiency of legume-rhizobium.

The present application relates to the technical field of biological medicine, in particular to streptomyces AH1901-2 and application thereof, preparation method and application of naphthymycin compounds, and belongs to the field of biological medicine. The streptomyces AH1901-2 is isolated from rhizosphere soil of Ligularia sibirica in Tianshan region (5.8°E, 28.0°N) in Xinjiang, and naphthymycin compounds are isolated and purified from liquid fermentation liquor of the streptomyces AH1901-2, and subsequent studies show that the naphthymycin compounds have obvious inhibitory effect on NO production of mouse mononuclear macrophages, and can be used for preparing anti-inflammatory drugs and treating inflammation.

The invention belongs to the technical field of microorganisms, and discloses an amorphophallus bulbifer southern blight infection influence analysis method based on metabonomics and microbiomics. Conjoint analysis shows that core florae (such as Streptomyces, Penicillium and Bauldia) are in significant positive correlation with phenylpropanoids and glutathione (succinic acid, coniferyl alcohol and glutamic acid), succinic acid is in positive correlation with Kribbella and Terrimonas, 2 ', 2' ', 2' ', 2' ', 2' ', 2' ', 2' ', 2' ', 2' ', 2' ', 2' ', 2' ', 2' ', 2' ', 2' ', 2' ', 2' ', 2' ', 2' ', 2' ', 2' ', 2' ', 2' ', the 3, 3 '-cycle CMP is negatively correlated with pathogen-related bacteria, which indicates that the metabolite regulates and controls the abundance of specific bacteria through positive / negative feedback so as to drive the microecological balance of rhizosphere, and a collaborative defense strategy that plants recruit beneficial microorganisms and inhibit pathogenic bacteria through metabolism reprogramming is disclosed.

The invention belongs to the technical field of organic substrates, and particularly relates to an organic substrate preparation process based on complex microbial inoculant synergistic fermentation, which comprises the following steps: selecting agricultural wastes, livestock and poultry manure and food industry byproducts, and carrying out staged fermentation by adopting complex microorganisms such as bacillus, streptomyces, clostridium and bacteroides; the decomposition efficiency of degradation-resistant substances such as cellulose and lignin is obviously improved. By combining precise temperature control and oxygen concentration adjustment strategies, the stability of the fermentation process is enhanced, nitrogen volatilization is reduced, and the total nitrogen content is stabilized at 1.2-1.5%. Nitrogen-fixing bacteria and phosphate solubilizing bacteria are introduced in the after-ripening stage, the biological activity of the matrix is improved, the screening process ensures that the finished product is uniform in particle size and few in impurities, the content of organic matter reaches 45% or above, the pH value is stabilized at 6.5-7.5, excellent agricultural performance is achieved, and efficient resource utilization of organic waste is achieved; the problems that in the prior art, organic matter degradation is not thorough, the controllability of the fermentation process is poor, nutrient loss is serious, and the quality of a finished product is unstable are effectively solved.

The invention belongs to the field of biological pharmacy, and particularly relates to a chalcanthicidin compound and application thereof. The invention discloses a chalcanthicidin compound which is separated from a secondary metabolite of marine actinomycetes Streptomyces sp. KCB-132 (marine actinomycetes sp. KCB-132). Pharmacological activity experiments show that the compound has a moderate inhibition effect on ESKAPE pathogenic bacteria with multiple drug resistance, such as enterococcus faecium, staphylococcus aureus, klebsiella pneumoniae, acinetobacter baumannii and escherichia coli. Therefore, the compound has the potential of being developed into a novel antibacterial agent.

The invention discloses genetically modified streptomycete and a streptomycete fermentation method for increasing the yield of secondary metabolites. A certain amount of lipoic acid is added into a streptomycete culture medium, so that the preparation is convenient, a certain promoting effect is achieved on secondary metabolic synthesis of the streptomycete, and the growth of strains is not influenced. According to the method disclosed by the invention, the lipoic acid fed-batch concentration suitable for a certain strain of streptomyces can be more accurately explored by exploring fed-batch modes at different time and different concentrations. According to the invention, lipoic acid synthetase gene conjugational transfer is introduced into streptomycete, and the synthesis of endogenous lipoic acid is enhanced by using an overexpression system, so that the synthesis capability of secondary metabolism of streptomycete can be generally enhanced.

The present application belongs to the field of microbial pesticides, and relates to a bacillus megaterium NBNC-104 for preventing and treating root-knot nematodes, a compound microbial agent and application thereof. The preservation number of the bacillus megaterium strain NBNC-104 is CCTCC M 20242324. Laboratory bioassay data show that the bacillus megaterium NBNC-104 has high toxic killing activity and repellent effect on southern root-knot nematodes, and has synergistic nematode killing activity after being compounded with bacillus thuringiensis strain NBIN-863 and kunming streptomyces sp. NBNH-006, and the strain can effectively colonize in the rhizosphere soil environment within 30 days under potting conditions. Therefore, the strain has broad application prospects in agricultural production.

The invention discloses an aromatic polyketone compound as well as a preparation method and application thereof. The compound GaB (3) has an effect of treating infection of multiple drug-resistant bacteria. The compound is obtained by being separated from a secondary metabolite of the symbiotic Streptomyces sp.QHH-9511 of licheniformis, and the compound 2, the compound 3, the compound 4 and the compound 5 are new compounds. According to the present invention, the research results show that the GaB can effectively sterilize a variety of drug-resistant bacteria (methicillin-resistant staphylococcus aureus, vancomycin-resistant enterococcus, carbapenem-resistant acinetobacter baumannii, multi-drug-resistant pseudomonas aeruginosa, multi-drug-resistant salmonella, and the like); in addition, the compound has the characteristics of broad-spectrum sterilization, biofilm formation inhibition and low-frequency drug resistance, and can be combined with other antibiotics to cope with super bacteria. Mechanism research shows that GaB is combined with glucosamine-6-phosphate synthetase (GlmS) to prevent synthesis of cell walls so as to play an antibacterial role, and a plurality of constructed living body models show that the compound can effectively reduce the content of bacteria in a body and promote wound repair and has in-vivo medication safety. The research result shows that the GaB can be used as a safe and effective novel pilot natural active small molecule for resisting infection of multiple drug-resistant bacteria.

The present invenon relates to the field of sustainable agriculture. More parcularly, the present disclosure provides composions and methods for natural pest management, more parcularly for inhibing or suppressing growth of unwanted plant species by making use of bioherbicides. The bioherbicide composions of current applicaon comprise one or more natural compounds produced by bacterial strains from the Streptomyces chrestomycecus species.

The invention belongs to the technical field of microbiology. The embodiment discloses a Streptomyces manniprovofaciens strain, a culture, a composition, a microbial agent and application thereof. The bacterial strain is a bacterial strain preserved in Guangdong Microbial Culture Collection Center with a preservation number of GDMCC No.64195, or a bacterial strain with a 16S rRNA gene sequence having at least 98.65% identity with a sequence represented by SEQ ID No: 1, or a bacterial strain with a genomic sequence having at least 95% average nucleotide consistency with the bacterial strain with the preservation number of GDMCC 64195, or a bacterial strain with a comparison fraction of at least 60%. The bacterial strain MN327951 provided by the embodiment of the invention shows relatively strong antibacterial activity and has a good siderophore producing characteristic. The strain MN327951 fermentation broth can significantly weaken the pathogenicity of ralstonia solanacearum to tomato seedlings, and the supernatant of the strain MN327951 fermentation broth has strong toxicity to Frankliniella occidentalis.

The invention provides a streptomyces isothiocyanate strain YC3071 and application of the streptomyces isothiocyanate strain YC3071 in tobacco disease prevention and control, and belongs to the technical field of biological prevention and control. The preservation name of the streptomyces isothiocyanate strain YC3071 is Streptomyces althioticus YC3071, the Latin name is Streptomyces althioticus, and the streptomyces isothiocyanate strain YC3071 is preserved in Guangdong Microbiological Culture Collection Center, and the preservation address is Institute of Microbiology, Guangdong Academy of Sciences, Floor 59, No. 100 Courtyard, Xianlie Middle Road, Guangzhou; the preservation date is July 4, 2024; the preservation number of the strain is GDMCC NO: 64829. The YC3071 strain has the characteristics of low use cost, good control effect, no residue and safety to human, livestock and environment, and the control effect of the microbial agent prepared from the YC3071 strain on tobacco black shank and tobacco brown spot is more than 70%.

The invention discloses streptomyces coronatoxin DB-2408, a metabolite of the streptomyces coronatoxin DB-2408 and application of the streptomyces coronatoxin DB-2408 to preparation of a medicine for treating non-small cell lung cancer, and belongs to the technical field of biological medicine. The invention provides a streptomyces coronatoxin DB-2408. The streptomyces coronatoxin DB-2408 can produce schizoxanthomycin through metabolism. Experiments prove that the compound can highly selectively inhibit a TRPV1 channel, calcium influx is reduced by 28%, and experimental results prove that the compound is low in toxicity under effective concentration and has the survival rate gt of cells with low expression of TRPV1; the compound has the activity of inhibiting the non-small cell cancer, provides a novel broad-spectrum treatment strategy for the NSCLC, and has a wide and good market application prospect.

The invention provides a preparation method and application of methyl 2-hydroxyphenylacetate. The methyl 2-hydroxyphenylacetate is separated from a fermentation culture of streptomyces wetland 13-3 with the preservation number of CGMCC (China General Microbiological Culture Collection Center) No. 22830. According to the method, the compound methyl 2-hydroxyphenylacetate is obtained from streptomycete for the first time, verification proves that the methyl 2-hydroxyphenylacetate has broad-spectrum antibacterial activity on various plant pathogenic fungi, particularly, the inhibitory activity on aspergillus fumigatus, colletotrichum truncatum, rhizoctonia solani, verruca verrucosa and magnaporthe oryzae is higher than that of amphotericin B, and the methyl 2-hydroxyphenylacetate can be used for preparing antibacterial agent drugs and has broad-spectrum antibacterial activity. A novel natural antibacterial compound is provided for the fields of agriculture and medicine, and the method is simple and convenient to operate, high in purification efficiency and environment-friendly.

The present application provides a kind of microbial iron reduction coupling biomass carbon soil carbon sequestration-organic pollution control collaborative remediation method, the present application method is by selecting including nitrogen-fixing bacteria, phosphorus solubilizing bacteria and iron-reducing bacteria Compound bacterial species, mixed with biomass carbon to form microorganism-biomass carbon compound, apply to soil, regulate soil environmental conditions, to promote iron cycle and the carbon sequestration effect of biomass carbon.The specific steps include: selecting nitrogen-fixing bacteria consisting of Clostridium pasteurianum and soybean rhizobium, phosphorus solubilizing bacteria consisting of Bacillus subtilis, flavobacterium or Streptomyces, and iron-reducing bacteria consisting of Geobacter;Prepare biomass carbon;Mix compound bacterial species with biomass carbon;Application and adjust soil pH, moisture content and aeration.The method is suitable for various soils, especially soils susceptible to MCPA pollution, can effectively improve the soil carbon sequestration capacity and reduce environmental pollution.

The invention belongs to the technical field of antiviral drugs for livestock and poultry, and discloses streptomyces LM-35, a metabolite of the streptomyces LM-35 and application of the streptomyces LM-35 in preparation of drugs for treating or preventing porcine reproductive and respiratory syndrome virus infection. A fermentation supernatant of the streptomyces LM-35 is used for carrying out anti-infection research on PRRSV at a cell level and an animal body level, and the streptomyces LM-35 is found to have remarkable anti-PRRSV activity in vivo and in vitro. The survival rate of the pigs fed with the streptomyces-containing LM-35 fermentation liquor reaches 80%; the adverse effect of PRRSV infection on the body can be effectively controlled, and the method can be used for preventing and controlling the PRRSV.

The invention belongs to the technical field of biological prevention and treatment, and particularly relates to application of streptomyces aureoflavus in prevention and treatment of beet brown spot. The invention provides an application of more than one of the following products in prevention and treatment of beet brown spot and / or beet root rot: (1) Streptomyces aureoflavus, (2) Streptomyces aureoflavus, (3) Streptomyces sp., (4) Streptomyces sp., (5) Streptomyces sp., (6) Streptomyces sp., (6) Streptomyces sp., (7) Streptomyces sp. The preservation number of the streptomyces aureoflavus is CGMCC (China General Microbiological Culture Collection Center) No. 28506, and the preservation number of the streptomyces aureoflavus is CGMCC No. 28506. (2) preparing a fermentation supernatant of streptomyces aureoflavus; (3) preparing a fermentation culture of streptomyces aureoflavus; and (4) preparing an extract of a fermentation supernatant of streptomyces aureoflavus. A pot experiment shows that the yield of the beet can be reduced by 42.585% due to the occurrence of the beet brown spot, and the biocontrol strain CYS4-5 can promote the yield of the beet to be increased by 46.122% while preventing and treating the beet brown spot disease.