993 results about "Streptomyces" patented technology

The invention discloses a compound microorganism liquid bacterial fertilizer for preventing plant diseases and insect pests and a preparation method of the compound microorganism liquid bacterial fertilizer and belongs to the technical field of microorganisms. The bacterial fertilizer comprises raw materials of, by weight, 10-20 parts of paprika powders, 10-20 parts of chilli powders, 5-15 parts of ginkgo leaf powders, 5-15 parts of chinaberry powders, 5-15 parts of lightyellow sophora root powders, 20-40 parts of mashed garlic, 40-80 parts of molasses, 4-8 parts of soybean meal, 5-15 parts of calcium sulfate, 5-15 parts of magnesium chloride, 5-10 parts of salt, 5-15 parts of photosynthetic bacterium strains, 5-15 parts of saccharomycete strains, 5-15 parts of lactic acid bacterium strains, 5-15 parts of streptomyces jingyangensis strains, 5-15 parts of bacillus subtilis strains and 800 parts of water. According to the compound microorganism liquid bacterial fertilizer, probiotic floras which are screened and are capable of coexisting are used for fermenting plant leaching agents of pepper, chilli, ginkgo leaves, chinaberry, lightyellow sophora roots and garlic, the plant leaching agents are capable of expelling and killing the insect pests and harmful bacteria, the compound microorganism liquid bacterial fertilizer has the advantages of being high in content of live bacteria of probiotics, high in activities, high in stability, small in using amount of plant materials, fast in acting effects, non-toxic and environment-friendly, and has wide application prospects in agricultural planting.

The present invention provides a tnaluronidase. The hyaluronidase can be produced by the strain Streptomyces aitinocidm 77, Exemplary characteristics of the hyaluronidase include specific C-terminal or other amino acid sequences, including full-length sequences, and improved physico-chemical and actix itj properties as compared to known h> alυronidase preparatkiiis. Described are also various uses of the hyaiurυnidasc, including topical administration of the h> aiuronidasc to improve skin penetration of a co-administered active substance.

The invention discloses a strain of Streptomyces and application thereof. The biocontrol Streptomyces is blastmycin Streptomyces and its strain number is JZB130180, which is registered in the general microbiological center of China Committee for Culture Collection of Microorganisms with CGMCC No. 13270. The strain had significant antagonistic effect on 14 kinds of plant pathogenic fungi and 6 kinds of plant pathogenic bacteria in agricultural production, and has wide antimicrobial spectrum. The strain produces chitinase, cellulase, protease and selenium, and has the basic characteristics of biocontrol bacteria. The fermentation broth of this strain has strong control effect on tomato gray mold and peach brown rot, and has significant promotion action on the tomato plants, which indicated that the strain had a broad application prospect in the field of biological control of plant diseases.

The invention discloses a composite microbial inoculum organic multielement compound fertilizer which is prepared from microbial inoculum, organic component and inorganic fertilizer. The microbial inoculum is a composite microbial inoculum which comprises the following three types of microbial inocula: resource type microbial inoculum, which contains Bacillus megaterium, thiobacillus thiooxidans, Bacillus mucilaginosus and Bacillus circulans; environment-friendly microbial inoculum, which contains Arthrobacter and Alcaligenes; and antibiotic microbial inoculum, which contains Streptomycs micuoflavus and Streptomyces jingyangensis. The organic component is one or more of cereal crop straw, subsidiary agricultural product dreg, sugar mill bagasse, bran coat, municipal and rural domestic waste, and livestock and poultry dung. The inorganic fertilizer is one or more of nitrogen fertilizer, phosphate fertilizer, potash fertilizer and medium / micro-nutrient element fertilizer. The weight proportion of microbial inoculum to organic constituent to inorganic fertilizer is (5-25):(18-28):(47-77).

A microbial leaven used for preparing organic or microbial fertilizer from organic waste is prepared from at least two of nine microbes. Aspergillus, Sporotrichun, Mucoraceae, Penicillium sp, Ceotrichum sp., Neurospora, Bacillus, Saccharomyces and Streptomyces sp.

The invention discloses a high-yield strain streptomyces lydicus, as well as breeding and fermentation thereof. The fermentation of the high-wield stain streptomyces lydicus E9 CGMCC No.3075 comprises the following steps: (1) preparation of a shake flask, seeds and a fermentation medium; (2) shake flask culture; (2) seed culture; (4) fermentation pot culture: leading a seed culture liquid in a fermentation pot filled with the fermentation medium and culturing for 24-96 hours to obtain a high-wield stain streptomyces lydicus E9 fermentation liquor under the condition of ventilating, stirring, temperature of 25-35 DEG C, and pH of 4-9; and carrying out ultrasonication for 20-40 min. The strain of the invention can generate large quantity of substances with antimycotic activity in the fermentation liquor, and the fermentation liquor has evident inhibiting effect on rhizoctonia solani; and the bacterial inhibition rate of the fermentation liquor which is diluted by 50 times reaches above 97.8% after 48 hour fermentation.

A new antibacterially active agent has been isolated from Streptomyces clavuligerus. This new compound which is designated clavulanic acid has the formula (I):In addition to being a broad spectrum antibiotic of medium potency, clavulanic acid and its salts and esters have the ability to enhance the effectiveness of beta lactam antibiotics against many beta-lactamase producing bacteria.

The invention provides a biological organic fertilizer prepared by aerobic fermentation of a biogas residue and a preparation method thereof. The preparation method comprises the steps: mixing the biogas residue and a swelling agent to form a fermentation raw material, inoculating a compound microbial agent in the fermentation raw material, uniformly mixing for fermenting, and adding urea, diammonium phosphate, a potassium chloride inorganic salt, brown nitrogen-fixing bacteria, mycorrhiza fungi, silicate bacteria, photosynthetic bacteria acetic bacteria, bifidobacterium, and saccharomycetes after the fermentation is completed to prepare the biological organic fertilizer, wherein the compound microbial agent comprises strains of bacillus, pseudomonas, staphylococcus, streptomyces, penicillium, aspergillus and trichoderma. The compound microbial agent prepared by using the biological organic fertilizer has a pertinence to aerobic fermentation of the biogas residue, and is capable of effectively increasing the fermentation rate of the biogas residue, shortening the fermentation time and realizing high-additional value production, innocent treatment and recycling of the biogas residue; and the problem of resources and environment of villages and small towns is solved, and the rapid development of construction of new countryside and towns and cities can be promoted.

A new antibacterially active agent has been isolated from Streptomyces clavuligerus. This new compound which is designated clavulanic acid has the formula (I): In addition to being a broad spectrum antibiotic of medium potency, clavulanic acid and its salts and esters have the ability to enhance the effectiveness of beta -lactam antibiotics against many beta -lactamase producing bacteria.

The invention relates to a preparation and the preparation method for restoring the microecological bacteria on the interface layer of water-sediment. The microecological bacteria preparation uses 0.2-0.4mm of medical stone as carrier, absorbs and fixes the composite microbe optimally combined by bacillus subtilis, bacillus licheniformis, bacillus megatherium, and thin yellow streptomyces. The amount ratio of bacillus subtilis to bacillus licheniformis to bacillus megatherium approximates to one to one to one; the volume ratio of the mixture liquid of the three kinds of bacillus to the thin yellow streptomyces liquid is two to one. The invention has the advantages that spreading the preparation of the invention into the interface layer of functional water body water-sediment in the spring March to April each year can prevent blue algae (aerugo micro-capsule algae is the most) from fulminantly growing in summer June to August. The preparation can improve the quality of water, can prevent eutrophication development, and can promote the carbon, nitrogen, and oxygen circulation on the interface layer of water-sediment. The microecological bacteria preparation has high effective colony amount and strong adaptability; and is effective to restore the microecological environment at the interface layer of water-sediment.

The invention discloses a complex microbial preparation for resisting replant obstacle. The complex microbial preparation comprises lactobacillus plantarum, bacillus megaterium, saccharomyces cerevisiae, streptomyces albus and rhodopseudomonas palustris, and is prepared by co-culturing the bacterial components by anaerobic and aerobic combined fermentation technology, wherein the complex microbial preparation comprises the following bacterial components in percentage by weight: 15 to 35 percent of lactobacillus plantarum, 5 to 25 percent of bacillus megaterium, 10 to 30 percent of saccharomyces cerevisiae, 15 to 30 percent of streptomyces albus and 10 to 25 percent of rhodopseudomonas palustris.

The invention belongs to the technical field of environmental microorganisms, and especially relates to an organic material decomposing agent and its preparation method. The preparation method comprises the following steps: preparing strains, drying, crushing, sieving, and mixing 35-55 parts of Bacillus spp with 2-5 parts of yeast, 5-10 parts of aspergillus, 4-20 parts of Streptomyces thermophilus, 8-15 parts of Thermoactinomyces sp and 20-30 parts of high temperature cellulose decomposition bacteria. The organic material decomposing agent can utilize easy-degradation components comprising starch, proteins and the like in a compost in order to accelerate the heating of the compost, and can also secrete digestive enzyme to accelerate the decomposition of difficult-decomposition substances comprising pectin, cellulose, lignin and the like without interfering the full performance of the microorganisms, so the composting efficiency and the quality of a composted finished product are improved, and the organic material decomposing agent has the advantages of low price, wide application range, strong practicability and easy popularization.

A highly specific and easily purified form of hyaluronidase is described for use in ophthalmic treatments. The enzyme, from Streptomyces hyalurolyticus is specific for hyaluronidase and carries out an elimination reaction that results in the production of double bonds at the nonreducing end of hyaluronic acid. Hyaluronidase from Streptomyces hyalurolyticus has a higher activity than comparable enzymes from other species. The enzyme is now capable of being purified in what is essentially a protease-free form making it applicable to medical treatments. The use of this source of hyaluronidase in ophthalmic treatments is now made possible by its high activity, specificity for hyaluronidase and purity.

Recombinant organisms are provided comprising genes encoding aquacobalamin reductase, cob(II)alamin reductase, cob(I)alamin adenosyltransferase, glycerol dehydratase and 1,3-propanediol oxidoreductase activities useful for the production of 1,3-propanediol from a variety of carbon substrates. More specifically the following nucleotide sequences are provided: btuR, encoding the E. coli cob(I)alamin adenosyltransferase enzyme; cobA, encoding the Salmonella typhimurium cob(I)alamin adenosyltransferase enzyme; cobO, encoding the Pseudomonas denitrificans cob(I)alamin adenosyltransferase enzyme; dhaB1, encoding the α subunit of the Klebsiella glycerol dehydratase enzyme; dhaB2, encoding the β subunit of the Klebsiella glycerol dehydratase enzyme; dhaB3, encoding the γ subunit of the Klebsiella glycerol dehydratase enzyme; dhaT, encoding Klebsiella oxidoreductase enzyme; the yciK gene isolated from E. coli; the glucose isomerase promoter sequence from Streptomyces; and the N-terminal amino acid sequence for cob(II)alamin reductase from Pseudomonas denitrificans.

The invention discloses a strain of Streptomyces lydidii and its application. The strain is Streptomyces lydicus A01 CGMCC No.1653. The bacterial strain can produce natamycin and will be widely used in the preparation of inhibitors of natamycin-sensitive bacteria.

The invention is suitable for the technical field of recycling organic wastes, and discloses a flora for recycling treatment of organic wastes. The flora for recycling treatment of organic wastes is efficiently compounded from a variety of bacteria such as bacillus subtilis, aspergillus niger, saccharomycete and soil flora, wherein the soil flora is extracted from the natural world and comprises three or more of bacillus megatherium, bacillus mucilaginosus, bacillus licheniformis, bacillus thuringiensis, bacillus laterosporus, bacillus mucilaginosus, streptomyces jingyangensis, mycorrhiza fungi, azotobacter vinelandii, photosynthetic bacteria, bacillus coagulans, aspergillus oryzae, and paecilomyces lilacinus. According to the flora for recycling treatment of organic wastes, disclosed by the invention, by virtue of mutual promotion, mutual coordination and combined action of a variety of beneficial microorganisms, the organic wastes are recycled, environmental pollution is reduced, and wastes are changed into valuables; and the flora for recycling treatment of organic wastes can be used for producing industrial fermentation products and improving the health and growth conditions of animals, and can also improve the current situation of serious soil hardening at present and improve soil quality, thereby promoting the rapid development of agricultural economics.

The invention discloses a complex microbial agent for composting fermentation and application thereof. The complex microbial agent is a solid inoculant which comprises phanerochaete chrysosporium, streptomyces griseorubens, bacillus subtilis, bacillus methylotrophicus and bacillus amyloliquefaciens. The complex microbial agent is a complex microbial system comprising various microorganisms which have complementary and synergistic functions; the microbial system integrates quick fermentation heat production, biological deodorization and compost material decomposition and degradation and has the effects of a biological fertilizer; the livestock manure compost can be quickly heated to kill off harmful organisms at high temperature; the unpleasant smell is obviously reduced to solve the problem of environment pollution caused by poultry excrement; the microorganisms can be quickly propagated in the poultry excrement fermentation process; the organic matters such as lignocellulose in the excrement can be quickly and effectively decomposed so as to increase the decomposition speed of the compost material and improve the quality of the compost product; the complex microbial agent can be used for producing the biological organic fertilizer so as to reduce the agricultural production cost.

The invention discloses a saline-alkali soil improved microbial agent and a preparation process thereof, and belongs to the field of saline-alkali soil improvement. The saline-alkali soil improved microbial agent is prepared from the following raw materials in parts by weight: 140-180 parts of a composite microbial agent, 100-130 parts of biochar, 30-50 parts of humic acid, 5-10 parts of a trace element, 10-15 parts of a filler and 30-50 parts of superphosphate. Synergistic growth and reproduction in a saline-alkali soil environment is achieved by combining bacillus subtilis, bacillus licheniformis, bacillus laterosporus, Jingyang streptomyce and bacillus mucilaginosus in a specific ratio. The combination of the kinds of microbial agents can improve synergistic alkali-resistance, the saline-alkali soil improved microbial agent can ferment well in saline-alkali soil, the growth of plants on saline-alkali soil is supported, biochar made from crop straws can improve the soil, help the growth of the plants, increase agricultural productivity and purify water quality, and is lower in cost relative to peat, and the saline-alkali soil improved microbial agent is worthy of promoting.

A streptomyce shygeroscopicus (CCTCC No.M203062) able to generate glutamine transaminase wigh high output is extracted from the soil of milk cow farm through plate screening, primary fermenting, flask culture, comparing test with standard color tape, and enzyme activity test. Said glutamine transaminase is prepared by deep fermentation.

The invention relates to an antibiotic streptomyces, fermentation product of which can inhibit broad spectrum fungus and protozoan, and field experiment can control crop fungus plant disease and root-knot nematode. The antibiotic streptomyces has good effect, no pollution nuisance and no residue. The antifungal agent has extremely low hazardness for the people and good performance and development prospect for medical and agricultural use. The invention is named as SIM001 antibiotic streptomyces which is Streptomyces antibioticus subspecies xi an, and is preserved as patent in the depositary institution appointed by patent office of state intellectual property office; the preservation data is April 14th, 2005, the name of the depositary institution is CGMCC, and the number of preservation is CGMCC No.1349. The whole cell hydrolysate of the antibiotic streptomyces contains L, L-DAP (Diaminopimelic acid) and no characteristic glucide; the cytoderm belongs to I type and glucide type C; one of the main antibacterial materials is polyene macrocyclic ketolide which belongs to the broad spectrum antifungal substance.

The invention provides a streptomyces fungicidious mutant strain FYFJ03 of which the collection number is CGMCC No.4113, the collection institution is China General Microbiological Culture Collection Center, and the collection date is August 25th, 2010. The invention also provides the application of the strain in the production of enramycin. In the mutant strain provided by the invention, threonine can be accumulated and the content of primer threonine for synthesizing the enramycin can be increased, so that the production level of the enramycin is improved.

The invention discloses a microbial agent, a preparation method thereof, and an application thereof, which relate to the technical field of microbial agent. The microbial agent includes Streptomyces albidofuscus CGMCC No.13717, Paecilomyces lilacinus CGMCC No. 13898, Streptomyces longus and mixed bacteria, wherein the mixed bacteria includes yeast, Bacillus subtilis, Bacillus licheniformis, Bacillus amyloliquefaciens, Brevibacillus laterosporus and Trichoderma harzianum. Through combination of bacteria-fungi and the synergistic effect of multi-strains, the microbial agent has the advantages ofhigh activity of strains, high efficiency and multi-functions in treating waste water, waste gas or waste. In addition, the invention also provides a preparation method of a microbial agent, which adopts a double-layer immobilization (preliminary immobilization and secondary immobilization combination) method of the microbial agent to improve the stability of the microbial agent. The preparationmethod is simple in operation and easy to be popularized and applied in industry.

The invention relates to a complex microbial inoculum for processing human and animal excreta efficiently, which consists of bacillus subtilis, bacillus licheniformis, geobacillus stearothermophilus, streptococcus thermophilus, geotrichum candidum, aspergillus niger and heat amylase streptomyces. A preparation method comprises the following steps of first-stage shake flask culture, second-stage shake flask culture, and third-stage solid medium culture of the complex microbial inoculum for the strains. The preparation method is simple with low use cost, saves resources, can be widely applied to stationary type or wheeled lavatories, and accords with the policy of sustainable development.

The invention discloses colibacillus and a method for performing soluble expression of transglutaminase proenzyme thereof. The method comprises the following steps: (1) designing PCR primer to perform PCR amplification according to the nucleotide sequence of transglutaminase proenzyme of streptomyces mobaraensis, cloning the related gene to prokaryotic expression vector pET22b (+),constructing expression vector pET22b-proMTG to conform to E.coli BL21 / proMTG of which strain preservation number is CCTCC M 208240; (2) performing soluble expression of transglutaminase proenzyme; (3) activating transglutaminase proenzyme; (4) performing high density fermentation; and (5) performing protein purification technology. In the method of the invention, the yield and specific activity of transglutaminase proenzyme can reach the level of that of transglutaminase proenzyme which is produced by firstly performing direct expression in colibacillus to form inclusion body and then performing denaturation and renaturation and the fermentation process is easier.

The invention discloses compound bactericide for treating sewage and a sewage treatment method thereof, and belongs to the technical field of sewage treatment. The compound bactericide comprises the following active ingredients from following strains: rhodopseudomonas, anaerobic ammonium oxidation bacteria, candida utilis, thiobacillus denitrificans, white-rot fungi, lactobacillus plantarum, bifidobacterium, lactobacillus acidophilus, green nonsulfur bacteria, phosphorus-accumulating bacteria, aspergillus oryzae, nitrosomas, streptomyces, bacillus subtilis and propionibacterium acidipropionici. The invention also provides the method using the compound bactericide to treat the sewage. The compound bactericide and the sewage treatment method have the advantages that the effect of treating sewage is better, the technology is simple, the implementing is easy, the operability is strong, the pollution to environment is avoided, the industrial requirements are met, and the application prospect is better.

A process is provided for the bioconversion of a carbon substrate to 1,3-propanediol by a single organism utilizing microorganisms, such as, Citrobacter, Enterobacter, Clostridium, Klebsiella, Aerobacter, Lactobacillus, Aspergillus, Saccharomyces, Zygosaccharomyces, Pichia, Kluyveromyces, Candida, Hansenula, Debaryomyces, Mucor, Torulopsis, Methylobacter, Escherichia, Salmonella, Bacillus, Streptomyces and Pseudomonas, containing the genes encoding for an active glycerol or diol dehydratase enzyme by contacting these organisms with a carbon substrate under the appropriate fermentation conditions. Specifically, Citrobacter and, Klebsiella provide the source of exogenous genes for such active dehydratase enzyme.

The invention relates to a biological seed-dressing agent, and in particular relates to a vegetable seed-dressing agent which contains soil beneficial microorganisms, relates to a method for preparing the biological seed-dressing agent, and belongs to the technical field of vegetable seed biological agents. The biological seed-dressing agent is mainly characterized by comprising bentonite which is used as a carrier medium, starch, hydroxymethyl cellulose and soil beneficial microorganism bacillus subtilis which are used as sticking agents, bacillus licheniformis and streptomyces. The biological seed-dressing agent has the effects of disease resistance, seed saving, seedling strengthening and the like as well as the advantages of no environmental pollution, safety in use, root growth and development promotion, crop drought resistance and adversity resistance enhancement, production increasing promotion and the like.

Genetically engineered Streptomyces clavuligerus strains with improved capabilities to produce clavulanic acid are provided. The strains are genetically engineered by disrupting newly identified glyceraldehyde-3-phosphate dehydrogenase (GAPDH) genes. This results in an increased intracelluar pool of the clavulanic acid precursor D-glyceraldehyde-3-phosphate (D-G3P), and increased clavulanic acid production. Clavulanic acid production may be further increased by supplying arginine to the medium in which the S. clavuligerus is grown.

The present invention relates to isolated strains of a Streptomyces spp. which are endophytes of dicotyledonous plants and to methods for selecting such strains. The present invention also relates to a biologically active compound called coronamycin obtained from endophytic Streptomycetes isolated from higher plants. The present invention further relates to compositions of such compounds and to methods of protecting plants against attack by a plant pathogen and methods of inhibiting bacterial growth, fungal growth, viral infection, growth of parasitic organisms, and cancer cell growth with such compositions.

The invention relates to citrus endophytic actinomycetes with antibacterial activity on various plant pathogens, belonging to the technical field of microbes. The endophytic actinomycetes are Streptomyces sp. R1020 which was registered and collected in China General Microbiological Culture Collection Center on March 17, 2014 with the collection number of CGMCC No. 8923. The strain R1020 has relatively good inhibitory effect on citrus anthrax bacteria, citrus bacterial canker disease bacteria, citrus penicillium italicum, citrus penicillium digitatum, bayberry deadwood disease bacteria, bayberry acicola, bayberry anthrax bacteria, loquat anthrax bacteria, loquat leaf spot fungi, loquat ringspot pathogenic bacteria, tomato alternaria solani, botrytis cinerea, colletotrichum orbiculare, tea tree anthrax bacteria and elsinoeleucospila and has the potential of being developed into biological pesticides.