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236 results about "Streptomyces thermodiastaticus" patented technology

Streptomyces thermodiastaticus is a bacterium species from the genus of Streptomyces. Streptomyces thermodiastaticus produces a lytic enzyme against Candida albicans.

Multifunctional active organic fertilizer and preparation method thereof

The invention provides a multifunctional active organic fertilizer and a preparation method thereof, relating to an organic fertilizer containing bacteria, fungi and spores, particularly an active organic fertilizer with functions of soil restoration and disease / pest resistance. The invention aims to provide a multifunctional active organic fertilizer capable of restoring soil pollution and improving product quality and a preparation method thereof. The multifunctional active organic fertilizer is composed of three layers from inside to outside, wherein the innermost layer is composed of a component A of which the main effective components are Bacillus subtilis and Paecilomyces lilacinus; the middle layer is composed of a component B and a component C containing multiple rich nutritive elements; and the outermost layer is composed of a component D of which the main effective components are Streptomyces vinaceus-drappus and Gliocladium roseum. The multifunctional active organic fertilizer can passivate heavy metals in soil, activate the soil, improve the microecological environment of the rhizosphere, degrade the chemical pesticides and improve the crop quality. The multifunctional active organic fertilizer is used in the field of agricultural planting.
Owner:廊坊华昌物联网科技有限公司

Microbial organic fertilizer as well as preparation method and application thereof

The invention discloses a microbial organic fertilizer as well as a preparation method and application thereof. The preparation method of the microbial organic fertilizer comprises the following steps of: mixing five types of bacteria liquid, including Klebsiella pneumoniae subspecies, Bacillus mucilaginosus, Bacillus megatherium, Bacillus subtilis and Thermophilic sporotrichum, with an organic matter base stock in a weight ratio of 1: 1: 1: 1: 1: 500 to obtain a mixture A and fermenting the mixture A, wherein the organic matter base stock is composed of poultry excrement, straws and humic acid-like substances in a weight ratio of 3: 3: 2; externally placing one bag of Streptomycesmicroflavus bacteria powder as B; and adding the bacteria powder of B into A according to the ratio of 0.2% to obtain the microbial organic fertilizer. The microbial organic fertilizer is applied to cotton and potato planting and grape planting under an extreme drought condition and the yield increasing effect is obvious. According to the invention, the technical difficulty of the fermentation of the existing microbial organic fertilizer is effectively solved, and nitrogen fixation, phosphorous decomposition and potassium decomposition effects are comprehensively achieved; and meanwhile, the microbial organic fertilizer is convenient for farmers, is simple and practical and has important application value.
Owner:新疆山川秀丽生物有限公司

Antibiosis streptomycete

The invention relates to an antibiotic streptomyces, fermentation product of which can inhibit broad spectrum fungus and protozoan, and field experiment can control crop fungus plant disease and root-knot nematode. The antibiotic streptomyces has good effect, no pollution nuisance and no residue. The antifungal agent has extremely low hazardness for the people and good performance and development prospect for medical and agricultural use. The invention is named as SIM001 antibiotic streptomyces which is Streptomyces antibioticus subspecies xi an, and is preserved as patent in the depositary institution appointed by patent office of state intellectual property office; the preservation data is April 14th, 2005, the name of the depositary institution is CGMCC, and the number of preservation is CGMCC No.1349. The whole cell hydrolysate of the antibiotic streptomyces contains L, L-DAP (Diaminopimelic acid) and no characteristic glucide; the cytoderm belongs to I type and glucide type C; one of the main antibacterial materials is polyene macrocyclic ketolide which belongs to the broad spectrum antifungal substance.
Owner:刘昶志

Streptomycete generating 5-keto-milbemycins and method for producing 5-keto-milbemycins

The invention relates to the technical field of gene recombination, and particularly relates to streptomycete generating 5-keto-milbemycins and a method for producing 5-keto-milbemycins. The invention provides a recombinant vector for knocking out the milF gene of streptomycete, including the homologous fragment of the milF gene, wherein the sequence of the fragment is obtained by losing 105-767 nucleotides from the nucleotides from the sites 158-924 in the nucleotide sequence shown by SEQ ID No.2. The invention also provides an establishment method of the recombinant vector, a method for knocking out the milF gene in streptomycete by use of the recombinant vector and streptomycete without milF gene. The streptomycete can be directly fermented to generate 5-keto-milbemycins, so that the synthesis process of milbemycin oxime is simplified, and the pollution caused by the traditional chemical synthesis method is avoided.
Owner:ZHEJIANG HISUN PHARMA CO LTD

Preparation method and application of high temperature-resistant and salt-resistant kitchen waste decomposing compound bacteria agent

ActiveCN108587967AHigh activityDelay the ripening processFungiBacteriaBacillus amyloliquefaciensMicrobiology
The invention discloses a preparation method of a high temperature-resistant and salt-resistant kitchen waste decomposing compound bacteria agent. The preparation method comprises the following steps:respectively performing seed culture on streptomycete, bacillus subtilis, bacillus amyloliquefaciens and saccharomyces cerevisiae, so that a streptomycete mycelium pellet suspension, a bacillus subtilis bacteria solution, a bacillus amyloliquefaciens bacteria solution and a saccharomyces cerevisiae bacteria solution are respectively and correspondingly obtained; inoculating the streptomycete mycelium pellet suspension to a solid fermentation material for culture to obtain solid decomposing bacteria; mixing the other three bacteria solutions to obtain a compound bacteria solution; stirring anduniformly mixing the compound bacteria solution and the solid decomposing bacteria according to the proportion of 9-11 ml / 100g, then drying at a low temperature, and finally adding an enzyme preparation for mixing to obtain the decomposing compound bacteria agent. The compound bacteria agent is used for decomposing kitchen wastes.
Owner:黄山中科新佳生物科技有限公司

Preparation and application of garden waste compound degrading microbial agent

ActiveCN105567612AEfficient and stable degradation abilityAccelerates the composting processBio-organic fraction processingBacteriaCelluloseStreptomyces thermocarboxydus
The invention discloses a compound microbial agent with a function of effectively degrading cellulose at high temperature. The compound microbial agent contains five actinomycetes: Streptomyces thermoviolaceus, Streptomyces thermodiastaticus, Streptomyces thermocarboxydus, Streptomyces albidoflavus and Streptomyces thermovulgaris, and one bacteria: Brevibacillus borstelensis. The compound microbial agent solves the problem of slow composting and long cycle of garden waste due to the limitation in cellulose degradation difficulty, accelerates cellulose degradation in the composting process, shortens the fermentation period, and improves the conversion efficiency, thereby having a broad application prospect in the aspects such as the application of composting treatment and resource utilization of garden waste, and the like.
Owner:BEIJING FORESTRY UNIVERSITY

Streptomyces samsunensis and application thereof

The invention provides actinomycetes which is streptomyces samsunensis XJC-2-6, and the preservation number is CCTCC NO:M 2017620. The streptomyces samsunensis XJC-2-6 provided by the invention has anantagonism on fusarium oxysporum f.sp cubense No. 4, curvularia fallax, curvularia lunata, btoryosphaeria dothidea, colletotrichum acutatum, colletotrichum gloeosporioides, colletotrichum fragariae,botrytis cinerea, fusarium oxysporum (schl.) f.sp cucumerinum owen, colletotrichum acutatum, pyricularia oryae cav, fusahum graminearum sehw, botryosphaeria dothidea and the like, has wide developmentspace on prevention and treatment of Panama disease of banana, microscler streak disease of banana, large grey speck disease of banana, tree canker of banana, anthracnose of pepper, anthracnose caused by colletotrichum gloeosporioides, anthracnose of strawberry, disease caused by botrytis cinerea, fusarium wilt of cucumber, anthracnose of mango, rice blast, wheat scab, ring rot of apple and the like, and has very good development and application prospects.
Owner:HAIKOU EXPERIMENTAL STATION CHINESE ACAD OF TROPICAL AGRI SCI +1

Special polyelement microorganism fertilizer for preventing yam anthracnose and preparation method of special polyelement microorganism fertilizer

The invention discloses a special polyelement microorganism fertilizer for preventing yam anthracnose and a preparation method of the special polyelement microorganism fertilizer. The special polyelement microorganism fertilizer comprises components of raw materials in parts by weight as follows: 2-4 parts of bacillus subtilis, 1-3 parts of frankia, 3-5 parts of streptomycete, 2-4 parts of nystatin, 50-60 parts of urea, 25-30 parts of ammonium phosphate, 15-20 parts of borax, Chinese herbal medicinal ingredients and the like. The special polyelement microorganism fertilizer can reduce the morbidity of the anthracnose, increase the yield of yams, and improve the soil condition.
Owner:南宁汉和生物科技股份有限公司

Streptomycesvinaceus-drappus microbial inoculum and method for preparing same

ActiveCN101812410ASignificant continuous cropping obstacle problemSuitable for continuous cropping obstaclesBiocideAgriculture tools and machinesDiseaseContinuous cropping
The invention provides a streptomycesvinaceus-drappus microbial inoculum. The streptomycesvinaceus-drappus microbial inoculum uses an active bacteria culture prepared by liquid submerged fermentation or solid fermentation of an active strain of streptomycesvinaceus-drappus, of which the storage number is CGMCC No. 2664, as an active ingredient. A tentative name of the streptomycesvinaceus-drappus microbial inoculum is S506, and the streptomycesvinaceus-drappus microbial inoculum was submitted to the CGMCC for storage on 11st September, 2008. The preparation method comprises the following steps of: A, activation of the strain; B, preparation of liquid seeds of the streptomycesvinaceus-drappus; and C, liquid submerged fermentation of the streptomycesvinaceus-drappus. The invention also provides a novel process of 'liquid seed preparation and solid fermentation' which is favorable for the growth of the streptomycesvinaceus-drappus, and the novel process meets the technical requirements on high effective viable count, high spore production rate, light contamination of crossbred bacteria and short fermentation period. When the active streptomycesvinaceus-drappus is applied to the roots of vegetables, various root diseases, such as blight, root rot and root knot nematode disease, of vegetables can be effectively prevented and controlled, and the streptomycesvinaceus-drappus microbial inoculum has obvious effects of enforcing growth of root systems and degrading autopoisonous matters of root systems, so that the streptomycesvinaceus-drappus microbial inoculum has a wide application prospect in effectively solving the problem of continuous cropping obstacles which are commonly happed to vegetables, fruit trees, medicinal materials and the like.
Owner:INST OF AGRI RESOURCES & ENVIRONMENT HEBEI ACADEMY OF AGRI & FORESTRY SCI

Synthetic method and extraction process of blue natural dye

A synthetic method and an extraction process of blue natural dye belong to the field of biological engineering. The purpose of the invention is to provide the synthetic method and the extraction process by using biological materials to synthesize non-toxic and environmental-friendly blue natural dye. The present invention uses purified brown chromogenic streptomycete genomic DNA as a template, uses a primer C1: 5'-aaTTAATTAAGGAGGAGCCCATatgagcgtagagaccatccc-3' and a primer C2: 5'-aaGCTAGCAAGCTTtcagtagttgggcgtcttgc-3' to was amplify indigoidine synthase gene SC-indC by a polymerase chain reaction, wherein the amplified SC-indC gene sequence is SEQ ID NO: 1. The method and the extraction process using engineering bacteria to synthesize blue natural dye indigoidine are high in yield, not restricted by sources of raw materials, easy to control the process, and low in production costs.
Owner:杭州唯铂莱生物科技有限公司

Livestock and poultry manure decomposing inoculant, use method thereof and biological organic fertilizer prepared by using livestock and poultry manure decomposing inoculant

The invention discloses a livestock and poultry manure decomposing inoculant, a use method thereof and a biological organic fertilizer prepared by using the livestock and poultry manure decomposing inoculant and fermenting through a special process. The livestock and poultry manure decomposing inoculant is prepared by compounding the following materials in parts by weight: 0.5-2 parts of bacillus subtilis, 0.5-2 parts of streptomyces microflavus, 0.5-2 parts of trichoderma longibrachiatum and 0.5-2 parts of bacillus mucilagimosus krassilm, wherein the effective viable count is 6*10<8> per gram. The livestock and poultry manure decomposing inoculant disclosed by the invention has multiple functions, can be used for decomposing the livestock and poultry manure and removing the odor and can resist the fusarium oxysporum and increase the yield after being applied to crops. When the livestock and poultry manure decomposing inoculant is applied to treating the livestock and poultry manure, the bacterial count, C/N ratio and fermentation period condition are all better than those of the comparison of an unknown inoculant. The biological organic fertilizer is prepared by adding 1 kilogram of the livestock and poultry manure decomposing inoculant to every 5 squares of the livestock and poultry manure and then fermenting through the special process. The livestock and poultry manure is formed by mixing the following materials in parts by weight: 40-60 parts of chicken manure, 20-40 parts of cattle manure, 5-15 parts of pig manure and 5-15 parts of lignite. The biological organic fertilizer disclosed by the invention is applied to scallion planting, achieves the disease control efficiency up to 56% and increases the yield per mu by 20.5%.
Owner:天蓝地沃(天津)农业科技有限公司

Streptomycete constitutive promoter and applications thereof

The invention discloses a streptomycete constitutive promoter and applications thereof, and provides an DNA fragment. The DNA fragment is any one selected from the following DNA molecules in 1)-3): 1) an DNA molecule represented by the sequence 1 in a sequence table; 2) an DNA molecule with promoter functions and hybrid with the DNA molecule shown in 1) under strict conditions; 3) an DNA molecule with more than 90% of homology with the DNA molecule shown in 1) and with promoter functions. The experiments shows that a strong constitutive promoter kasO*p is screened out, and the promoter and the erythromycin promoter ermE*p are compared in representation of transcriptional level and expression level in streptomyces coelicolor, streptomyces avermitilis and streptomyces venezuelae, and the results show that the modified promoter kasO*p is better than the the erythromycin promoter ermE*p.
Owner:INST OF MICROBIOLOGY - CHINESE ACAD OF SCI

Kitchen waste composite microbial degradation agent as well as preparation method and application thereof

The invention discloses a kitchen waste composite microbial degradation agent as well as a preparation method and application thereof. The degradation agent is prepared from Bacillus subtilis, Streptomyces thermodiastaticus, Bacillus stearothermophilus, Lactobacillus acidophilus, Saccharomyces cerevisiae, Enteromorpha protease, Methylobacterium radiora, Pantoea dispersa, Pseudomonas oryzihabitans,Citrobacter freundii and Staphylococcus cohnii. The mixed strain accounts for 15-25% of the total mass of the degradation agent, the mass percentage of water in the degradation agent is 10-20%, the pH value of the degradation agent is 5.5-8.5, and each gram of the degradation agent contains 106-109 viable bacteria. The degradation agent has the beneficial effects that: kitchen waste containing various substances such as vegetables, grains and meat can be effectively degraded, the activity of microorganisms in the degrading agent is high, the degrading rate reaches 80% or above, the degradingrate is high, the use amount is small when the kitchen waste is treated, generated peculiar smells are small, the cost is low, use is convenient, toxic and harmful substances are not generated, and safety, reliability and environment friendliness are achieved.
Owner:人与自然环保生物科技(南京)有限公司

Streptomyces minitype transposable mutation system as well as construction method and application thereof

The invention belongs to the fields of biotechnology and genetic engineering, in particular relates to a high-efficiency streptomyces minitype transposable mutation system as well as a construction method and application thereof. Based on a transposable element sourcing from Norcardia aseteroids YP21, the invention constructs the characteristics of action sites of IS204 transposase in streptomyces coelicolor, and the transposable system is proved to have good genetic stability and good transposable randomness. Transposable mutation is carried out on S. coelicolor M145 by utilizing pDZY101 and derivatives thereof to obtain a great quantity of phenotype mutant strains, in addition, similar characteristics are also found in streptomyces lividans and other streptomyces members, and the characteristics endow the transposable system with good application prospect in aspect of researching type streptomyces functional genes.
Owner:FUDAN UNIV

Method for producing seedling raising substrate by using cotton straw and substrate prepared with same

The invention discloses a method for producing a seedling raising substrate by using cotton straw and a substrate prepared with the same. The method comprises the following steps of: smashing cotton straw into sections of 10-50 millimeters; adding water till the water content is 60-80 percent according to mass percentage; adding 2-3kg of urea, 0.5-1kg of calcium superphosphate, 1-2kg of gypsum and 50-100g of fermentation inoculum into each cubic meter of substances obtained in the last step according to the volume ratio; mixing uniformly; and sealing, piling and fermenting at the air temperature of 15-40 DEG C, wherein the adopted compound fermentation inoculum is prepared by mixing pseudomonas aeruginosa, streptomyces albus and flavobacterium in the ratio of 2:3:1, and can be used by fermenting, airing in a scattered way and drying. In the method, a main raw material is derived from a large amount of waste cotton straw generated during the production of cotton, the raw material source is rich, a preparation process is simple, the aim of utilizing resources is fulfilled, and resource waste of cotton straw caused by direct smashing and returning to field can be reduced; and the method has a wide application value.
Owner:新疆万康诚一肥业有限公司

Method for producing tacrolimus by fermentation

The invention provides a method for producing tacrolimus by fermentation, belonging to the field of biomedicines. According to the method of producing tacrolimus by fermentation, parameters such as a strain-activating bevel culture medium component, a liquid culture medium component, seed liquid inoculum size and a fermentation medium component are optimized to maintain the growing activity and the fermenting activity of streptomycete, so that the production of tacrolimus is improved. In the fermenting process, piperidine acid is added into the fermentation medium and the adding manner such as adding time and addition is optimized, so that the feedback inhibition of products is reduced, and meanwhile, the output of the final product tacrolimus is increased, so that the final output of tacrolimus can reach 385 microgram / mL.
Owner:CHENGDU YATU BIOLOGICAL TECH

Solid fermentation process of streptomyces flavus and its production equipment

A solid fermenting process of xanthostreptomycete includes such steps as proportionally mixing soybean cake powder, peanut shell, corn flour, millet flour, wheat bran, and lime and water, high-pressure sterilizing at 128-135 deg.C for 60-90 min, inoculating seeds, and fermenting for 144 hr. Its apparatus is composed of dual-screw stirrer, disinfecting stainless steel tray, sterilizing carriage, high-pressure sterilizing pot, collectnig hopper, belt conveyer, humidify meter, fermenting tray and fermenting case.
Owner:青岛地恩地生物科技有限公司

Characteristic fertilizer for cigarette and production method thereof

The invention discloses a characteristic fertilizer for cigarette and a production method thereof. At present, it is common to increasingly use organic fertilizers for improvement of soil and increase the content of flavor matters in tobacco leaves in modern tobacco agriculture. The invention provides the characteristic fertilizer for cigarette capable of effectively promoting formation of dominant microflora at the root of flue-cured tobacco and the production method thereof. The method comprises the following steps: uniformly mixing oil cake, bacillus subtilis, bacillus licheniformis, sporotrichum thermophile and trichoderma viride with stirring and standing an obtained mixture for fermentation; adding phosphate dissolving bacillus megaterium and streptomyces jingyangensis and continuing fermentation; adding potassium releasing bacillus mucilaginosus and streptomyces coerulescens and continuing fermentation until the temperature of the mixture drops to normal temperature; carrying out granulation; preparing inorganic particles; carrying out mixing. The production method for the characteristic fertilizer for cigarette enables larva of mosquitoes and flies propagated in the process of maturing of organic materials to be killed and environment cleanliness of a fermentation place to be improved, and has the advantages of a simple production process and a short production period.
Owner:YUNNAN SOURCE DAY BIOLOGY GRP CO LTD

Soil improvement type composite biological organic liquid fertilizer

The invention belongs to the organic fertilizer technical field, in particular relating to a soil improvement type composite biological organic liquid fertilizer. The soil improvement type composite biological organic liquid fertilizer is prepared by combination of bifidobacterium, lactobacillus bulgaricus, lactobacillus casei, saccharomyces cerevisiae, bacillus subtilis, streptomyces hygroscopicus and so on. The compositions are all profitable strains sieved from soil, are cultured and multiplied by application of the biotechnology, added with organic substances and nutritive elements, and processed into the novel composite biological organic liquid fertilizer by means of chelating, and are favorable for recovering the ecosystem of soil organisms. Therefore, the product of the soil improvement type composite biological organic liquid fertilizer not only has the function of fertilization but also can improve the physical property and the biological property of the soil and have the function of improving the soil, and can be widely used for various crops.
Owner:上海丰廷生物科技有限公司

Tyrosinase coding gene melC derived from streptomyces kathirae SC-1 and proteins of tyrosinase coding genes melC

InactiveCN104404064AHas UV absorptionAntioxidantOxidoreductasesFermentationZymogenOperon
The invention relates to tyrosinase coding gene melC derived from streptomyces kathirae SC-1 and proteins of the tyrosinase coding genes melC. Streptomyces kathirae SC-1 is a high-yielding melanin strain obtained through early screening in the laboratory and is now kept in China Center for Type Culture Collection (CCTCC) with the preservation number of CCTCC M 2012432. The process provided by the invention finally obtains a cloned 1899bp sequence which includes a 553 bp upstream open reading frame sequence and tyrosinase operon melC (including two coding genes melC1 and melC2). The melC1 gene product is tyrosinase metallochaperone and has the functions of activating tyrosine zymogen, transporting copper ions to tyrosinase, helping tyrosinase move outside the cell. By comparing the tyrosinase encoding gene provided by the invention with that reported at home and abroad, the highest similarity degree between both is only 75%, and by comparing the encoded amino acid sequence provided by the invention with that reported at home and abroad, the highest similarity degree between both is only 83%. The comparison results prove that the tyrosinase coding gene melC derived from streptomyces kathirae SC-1 is a novel tyrosinase.
Owner:JIANGNAN UNIV

Microzyme for degrading residual lincomycin in fermented leaves and application thereof

The invention discloses a yeast strain, which is of a galactomyces geotrichum S9 strain. The galactomyces geotrichum S9 strain is collected in China General Microbiological Culture Collection Center on March 5, 2012, the collection and registration number is CCTCC M2012056, the galactomyces geotrichum S9 strain can degrade lincomycin and is derived from a certain metabolite component in a fermentation solution of the strain, and the main action mechanism is that the certain metabolite component generated by the strain can produce action on a certain key chemical bond in the structure of the lincomycin and further degrade the lincomycin into small molecular substances. Simultaneously, the galactomyces geotrichum S9 strain can grow rapidly under the culture conditions described by the invention, industrial production is easy to perform, the galactomyces geotrichum S9 strain has great application and development prospects, and the galactomyces geotrichum S9 strain has high efficiency and practicality when the galactomyces geotrichum S9 is used for treating the fermented leaves of streptomyces lincolnensis.
Owner:NANYANG NORMAL UNIV

Tetrahydroquinoline alkaloid Malaysiensin with immunosuppression activity and production method and application of tetrahydroquinoline alkaloid Malaysiensin with immunosuppression activity

The invention provides a tetrahydroquinoline alkaloid Malaysiensin with immunosuppression activity and a production method and application of the tetrahydroquinoline alkaloid Malaysiensin with the immunosuppression activity. The compound is obtained by conducting SFM solid medium fermentation on Streptomyces sp.DSM 4137 and separating and purifying a fermentation product. The compound can significantly inhibit ConA-induced proliferation of T cells, an immunosuppression effect of the compound is equivalent to that of an immunosuppressor cyclosporin A which is clinically and widely used, and action mechanism studies show that Malaysiensin exerts the immunosuppression activity for a targeted NFAT signal path, is an efficient immunosuppressor, and can be used for producing immunosuppressive medicines for organ transplantation and autoimmune diseases.
Owner:HAINAN UNIVERSITY

Recombinant streptomyces lydicus for producing cellulase and natamycin and application

InactiveCN104480135AImproved ability to degrade celluloseEnhanced ability to produce natamycinBiocideBacteriaErythromycin resistanceStreptomyces lydicus
The invention discloses recombinant streptomyces lydicus for producing cellulase and natamycin and an application. A construction method of recombinant streptomyces lydicus comprises steps as follows: expression vectors of an encoding gene of hemoglobin and an encoding gene of cellulase are guided into streptomyces lydicus to obtain the recombinant streptomyces lydicus for producing cellulase and natamycin; hemoglobin is protein of A 1) or A 2), in A 1), the amino acid sequence of hemoglobin is represented as SEQ ID No.3 in a sequence table, and in A 2), hemoglobin is protein which has the hemoglobin function and is derived from A 1); and cellulase is protein of B 1) or B 2), in B 1), the amino acid sequence of cellulase is represented as SEQ ID No.4 in the sequence table, and in B 2), cellulase is protein which has the cellulase function and is derived from B 1). The expression vectors comprises an erythromycin resistance gene promoter, the encoding gene of cellulase, an erythromycin resistance gene promoter and the encoding gene of hemoglobin in the direction from upstream to downstream.
Owner:BEIJING ACADEMY OF AGRICULTURE & FORESTRY SCIENCES

Biocontrol streptomycete

ActiveCN110699304ABroad antibacterial spectrumGreat potential for biological controlBiocideBacteriaBiotechnologyAnti bacterial
The invention relates to biocontrol streptomycete. The preservation number of the strain of the biocontrol streptomycete is CGMCC No.18658. During cultivation, the biocontrol streptomycete is inoculated to a fermentation medium for fermentation cultivation. The strain or a biocontrol product prepared through the strain can be used for plant pathogen preventing and treating. The biocontrol streptomycete or a biocontrol preparation prepared through the biocontrol streptomycete has the advantages of good antibacterial effect, broad antimicrobial spectrum, low toxicity, no residue, environmental friendliness, broad development prospect and the like.
Owner:JILIN CITY ACADEMY OF FORESTRY

Nosiheptide biosynthetic regulatory protein NosP specific binding sequence and related application thereof

The invention discloses a nosiheptide biosynthetic regulatory protein NosP specific binding sequence and an influence thereof upon nosiheptide biosynthesis effectiveness. The invention comprises the following contents: recombinant plasmids comprising specific affinity purification tags and truncated NosP gene are established; a NosP protein pure product is prepared through Escherichia coli heterologous expression and an affinity chromatography method; and with EMSA and DNase I footprinting assay technologies, the NosP specific binding sequence in a streptomyces actuosus nosiheptide biosynthetic gene cluster is found and verified. On this basis, through nosiheptide biosynthetic pathway renovation, nosiheptide biosynthesis effectiveness is improved by 150%, such that an application prospect is good.
Owner:CHINA PHARM UNIV

Special compound microorganism fertilizer for preventing yam anthracnose and preparation method of special compound microorganism fertilizer

The invention discloses a special compound microorganism fertilizer for preventing yam anthracnose and a preparation method of the special compound microorganism fertilizer. The special compound microorganism fertilizer for preventing the yam anthracnose comprises components in parts by weight as follows: 2-4 parts of bacillus subtilis, 1-3 parts of frankia, 3-5 parts of streptomycete, 2-4 parts of nystatin, 50-60 parts of urea, 25-30 parts of ammonium phosphate, 15-20 parts of borax, 10-15 parts of ferrous sulfate, 8-10 parts of manganese sulfate, 10-12 parts of zinc sulfate, 20-24 parts of calcium superphosphate, 80-100 parts of peanut cakes, 70-80 parts of a soybean meal, 100-120 parts of maize straw, 90-100 parts of expansion chicken manure, 40-50 parts of waste of a chicken slaughter house, 0.2-0.4 part of multi-effect triazole and 0.4-0.6 part of gibberellin. The special compound microorganism fertilizer can reduce the morbidity of the anthracnose, increase the yield of yams, and improve the soil condition.
Owner:SUZHOU RENCHENG BIO SCI & TECH

High-yield tacrolimus streptomycete mutant strain and application thereof

The invention provides a high-yield tacrolimus streptomycete mutant strain. The mutant strain is (Streptomyces tsukubaensis)FIM-07-23, and preserved at the common microorganism center of China Committee for Culture Collection of Microorganisms on January 7th, 2019, the address is No.3, courtyard 1, the western Beichen road, Chaoyang District of Beijing, and the preservation number is CGMCC No.17137; the (Streptomyces tsukubaensis)FIM-07-23 is applied into fermentation production of tacrolimus. The (Streptomyces tsukubaensis)FIM-07-23 can ferment high-yield tacrolimus, the yield of tacrolimus is greatly increased, and the genetic stability is good.
Owner:FUJIAN INST OF MICROBIOLOGY

Composite enzymolysis reagent for keratin enzymolysis and application method thereof

The invention discloses a composite enzymolysis reagent for keratin enzymolysis and an application method thereof. The composite enzymolysis reagent comprises streptomycete cutin protease, yeast enzyme and a reducing agent. The application method comprises the following steps: firstly, performing ungrease treatment on a natural raw material with cornifin, a yeast enzyme and a reducing agent; adding the natural raw material with the cornifin after ungrease treatment in the step 1) into a combined enzymolysis agent liquid, pretreating for 15-25 minutes at the room temperature without oxygen, further adjusting the pH value of the combined enzymolysis agent liquid to be 7.0-7.5, controlling the temperature to be 50-60 DEG C, and performing enzymolysis for 12-24 hours, thereby obtaining a keratinase liquid; decoloring and sterilizing the keratinase liquid; and performing vacuum drying, thereby obtaining protein powder. The composite enzymolysis reagent is rapid in enzymolysis and high in keratin degradation rate, and generation of toxic substances can be effectively prevented, so that the prepared protein powder is safe and non-toxic and high in nutrition component.
Owner:湖州珍贝羊绒制品有限公司

Biocontrol preparation for disease prevention of tobacco and preparation method thereof

InactiveCN109526986AAvoid the risk of ecological imbalanceSave biological resourcesBiocideFungicidesDiseaseBiotechnology
The invention relates to the technical field of plant biocontrol preparations, specifically to a biocontrol preparation for disease prevention of tobacco and a preparation method thereof. The invention aims to provide a biocontrol preparation for disease prevention of tobacco and a preparation method thereof. The preparation method comprises the following steps: evenly mixing a mixed medium freeze-dried powder, sugarcane powder and plant ash according to a certain ratio so as to obtain pulvis of the biocontrol preparation for disease prevention of tobacco; mixing the mixed medium freeze-driedpowder and some organic matter and dissolving by adding water so as to obtain the biocontrol preparation for disease prevention of tobacco. The mixed medium freeze-dried powder comprises medium freeze-dried powder of bacillus subtilis, Trichoderma harzianum and Streptomyces microflavus. The biocontrol preparation for disease prevention of tobacco can control some common but hazardous diseases of tobacco.
Owner:LINCANG OF YUNNAN TOBACCO +1

Method for biosynthesizing virginiamycin by streptomycete

The invention discloses a method for biosynthesizing virginiamycin by streptomycete, which comprises the following steps of: preparing a slope with spores of virginiamycin; inoculating the slope spores into a culture medium to obtain a seed solution; inoculating the seed solution into a fermentation culture medium for fermentation culture, adding a precursor substance at the early stage of fermentation so as to increase the yield of virginiamycin; and further processing the fermentation solution after fermentation to obtain virginiamycin crystals. The method disclosed by the invention is simple and easy to implement, is stable in the fermentation result and low in production cost. The fermentation result is improved compared with the traditional process, and the highest yield of virginiamycin can reach 3500 mu g / ml. Virginiamycin is polypeptide antibiotic containing lactonic rings, has the characteristics of no toxicity, no accumulation, easiness in degradation, and no drug resistance, and is a new animal feed additive.
Owner:江西兴鼎科技有限公司
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