132 results about "Streptomyces thermodiastaticus" patented technology

The invention discloses talaromyces purpurogenum as well as a fermented supernatant and application thereof. The preservation number of the talaromyces purpureogenus F-4 is CCTCC NO: M 20252023, and the talaromyces purpureogenus F-4 has the growth promoting effects of dissolving phosphorus, fixing nitrogen and generating siderophores, also has the disease preventing and treating effects of preventing and treating garlic root rot, potato scab and wheat stem rot, and also can resist salt stress, alkali stress, salt-alkali composite stress, heavy metal stress and osmotic pressure stress. The fermentation supernatant of the talaromyces purpureus F-4 can be used for inhibiting the growth of fusarium solani spores, streptomyces botrytis spores and fusarium pseudograminearum and the germination of the spores of the fusarium solani, the streptomyces botrytis and the fusarium pseudograminearum. The talaromyces purpurogenus and the fermented supernatant thereof can effectively control the growth and reproduction of plant pathogens, reduce the risk of disease occurrence, reduce the dependence on chemical pesticides, and reduce the risk of environmental pollution and agricultural product residues, and have important significance for promoting the sustainable development of agriculture.

The invention discloses a biocontrol complex microbial inoculant for preventing and treating maize curvularia leaf spot and application thereof, and belongs to the field of crop disease prevention and treatment. The biocontrol complex microbial inoculant comprises the following active ingredients: bacillus cereus, pseudomonas chlororaphis and streptomyces microflavus, and / or metabolites thereof. The beneficial microorganisms with different action mechanisms are combined, and the prevention and control effect on the curvularia maize leaf spot disease is enhanced through the synergistic effect. Natural microbial resources are adopted to replace traditional chemical pesticides, harmful substance residues are reduced, and the ecological environment is protected. Through reasonable matching of different types of beneficial microorganisms, effective prevention and control of the curvularia maize leaf spot disease are realized, and healthy growth of plants can be promoted. Compared with a single strain preparation, the complex microbial inoculant provided by the invention has stronger stability and durability, and can significantly reduce the incidence rate of diseases.

The invention relates to recombinant streptomyces avermitilis, a construction method of the recombinant streptomyces avermitilis and application of the recombinant streptomyces avermitilis in preparation of combined vermectin B. The recombinant streptomyces avermitilis is streptomyces avermitilis HU510 preserved in China Center for Type Culture Collection (CCTCC), the preservation date is October 9, 2024, and the preservation number of the strain is CCTCC NO: M 20242151. The invention relates to the technical field of synthetic biology, in particular to a method for preparing and producing a novel biological insecticide, and particularly relates to a method for preparing recombinant insect-killing streptomyces of combined vermectin B. The streptomyces sp. Has an aveA3 gene which is inactivated or reduced in activity, and has a functional milA3 gene. According to the invention, efficient production of the combined vermectin B can be realized. According to the invention, a biological synthetic method and technology are utilized to recombine and transform a biosynthetic pathway of abamectin, and the novel insecticidal antibiotic is obtained through screening.

The invention relates to streptomyces cell and application thereof in efficient degradation of aflatoxin B1, the streptomyces cell is streptomyces cell AFB1-D02, and has been preserved in China General Microbiological Culture Collection Center (CGMCC), the preservation number is CGMCC No.33986, and the preservation date is March 26, 2025. The invention further discloses a preparation method of the streptomyces cell. The streptomycete S. cellostatus AFB1-D02 has the capability of efficiently degrading AFB1, and the degradation efficiency of the streptomycete S. cellostatus AFB1-D02 is obviously higher than that of other streptomycetes in the prior art.

The invention discloses a marine streptomyces strain SHEa3, a compound derived from the marine streptomyces strain SHEa3 and application of the marine streptomyces strain SHEa3, the classification name of the marine streptomyces strain SHEa3 is Streptomyces sp, the preservation number of the marine streptomyces strain SHEa3 is GDMCC No: 66667, and the preservation date of the marine streptomyces strain SHEa3 is July 14, 2025. The invention also discloses a sesquiterpenoids compound streptaviol A derived from marine streptomyces and application of the sesquiterpenoids compound streptaviol A in preparation of drugs for preventing and treating vibrio diseases of mariculture animals. The invention further discloses application of the sesquiterpenoids, namely the sea threitol C, derived from the marine streptomyces in preparation of drugs for preventing and treating vibrio diseases of mariculture animals. The marine streptomyces source compound has efficient anti-vibrio activity, and the marine streptomyces source compound streptaviol A and the helithreitol C are used for preparing the medicine for preventing and treating vibrio diseases of mariculture animals, so that the side effect is low, and the activity is excellent.

The invention discloses a naphthyridine mycin compound generated by streptomyces as well as a preparation method and application thereof, and belongs to the field of medicines. The naphthyridine mycin compound and the analogue of the naphthyridine mycin compound are prepared by fermenting, extracting, separating and purifying streptomyces lageri W307 (the preservation number of the strain is CGMCC (China General Microbiological Culture Collection Center) No.32721). The invention further discloses a preparation method of the naphthyridine mycin compound and the analogue of the naphthyridine mycin compound. The invention further provides application of the eight naphthyridine mycin compounds in preparation of anti-tumor drugs and application of the eight naphthyridine mycin compounds in preparation of antibacterial drugs, and potential anti-tumor drugs and antibacterial drugs are provided clinically.

The invention discloses a collagenase mutant, a gene segment, a recombinant plasmid, a recombinant expression system and application thereof, and a method for preparing the collagenase mutant and collagen tripeptide, and belongs to the field of gene engineering and fermentation engineering. The invention provides a collagenase mutant KT231 capable of realizing high-efficiency secretory expression in streptomyces S.lividans TK24 and a preparation method of the collagenase mutant KT231. The collagenase mutant KT231 can be used for efficiently carrying out enzymolysis on the skin of the narrow gadus at 60 DEG C, has excellent heat resistance and higher enzyme activity, and can be used for efficiently preparing the collagen tripeptide from the skin of the narrow gadus. The collagen peptide is compounded with commercially available papaya chymotrypsin, so that the proportion of collagen tripeptide in the fish skin enzymatic hydrolysate can be remarkably increased, and the fish skin enzymatic hydrolysate has no bitter taste. The method has wide application prospects in preparation of products, tissue engineering materials and wound healing accelerators for resisting wrinkles, preserving moisture, repairing damaged skin and improving bone and joint health.

The invention provides a phytophthora nicotianae biocontrol actinomycete FK-1 and application thereof, and belongs to the technical field of biocontrol bacteria. The invention provides a streptomyces strain FK-1, and the preservation number of the streptomyces strain FK-1 is CCTCC (China Center for Type Culture Collection) NO: M 20242872. The streptomyces FK-1 disclosed by the invention shows remarkable broad-spectrum antibacterial activity on various phytopathogens, and has the capabilities of slowing down pathogen infection and reducing plant diseases. The inhibition rate of the streptomyces FK-1 to phytophthora nicotianae reaches 82.08%, the antibacterial activity of strain fermentation liquor can reach 85.88%, and the prevention and treatment effect of indoor potting is 80.65%; meanwhile, the phytophthora nicotianae hyphae can be deformed, wound and irregularly bent, so that the growth of the phytophthora nicotianae hyphae is inhibited, and the method can be applied to prevention and control of phytophthora nicotianae.

The invention provides a carbon-based bio-fertilizer, a preparation method thereof and application of the carbon-based bio-fertilizer in prevention and treatment of tomato bacterial wilt, and belongs to the technical field of biological prevention and treatment of plant diseases. The carbon-based bio-fertilizer for preventing and treating tomato bacterial wilt is prepared from the following raw materials in parts by weight: 22 to 28 parts of livestock manure, 10 to 15 parts of straw, 10 to 15 parts of mushroom dregs, 10 to 15 parts of charcoal, 6 to 9 parts of medicine dregs, 8 to 12 parts of potassium humate, 6 to 8 parts of pine sawdust, 3 to 6 parts of microbial agent and 4 to 6 parts of attapulgite powder. The microbial agent comprises paecilomyces lilacinus, streptomyces microflavus, bacillus megatherium, lactobacillus plantarum and photosynthetic bacteria. The carbon-based bio-fertilizer for preventing and treating tomato bacterial wilt is composed of different organic wastes and specific microbial flora, is not easy to generate drug resistance, can quickly form dominant flora after being applied to soil, and can effectively antagonize tomato bacterial wilt pathogenic bacteria and prevent and treat tomato bacterial wilt.

The invention provides a streptomyces graminearus strain, a streptomyces graminearus oil suspension, as well as its preparation method and application thereof. The streptomyces graminearus oil suspension contains streptomyces graminearus conidiospore powder, emulsifier, stabilizer, wetting dispersant, antioxidant, thickener, ultraviolet protectant, preservative and carrier oil. The use of carrier oil as the dispersion medium offers environmental friendliness, excellent permeability, adhesion properties, and resistance to rain washing. Additionally, the carrier oil enhances the spore germination of streptomyces graminearus. Furthermore, it has the advantages of low application cost, slow spore settling speed, easy to shake well when using, stable efficacy, long storage time, suitability for various spraying techniques and a simple preparation method. The spore survival time of the streptomyces graminearus oil suspension is prelonged, with a spore germination rate is as high as 85% after 18 months of storage. It demonstrates effective control against yam, peanut and forest nematode.

The invention provides a dynamic control system for coordinating streptomyces secondary metabolite production and application of the dynamic control system. Firstly, a general artificial promoter PQS (trigger) responding to a quorum sensing system of various streptomycetes is designed, then a stabilizer is constructed based on a bistable gene circuit, instantaneous signal output is converted into stable signal output, dependence of an inducer is successfully eliminated, finally, an orthogonal amplifier is developed based on a streptomycete rare extracellular function sigma factor, and finally, the specific function of the quorum sensing system of various streptomycetes is achieved. And different promoter element libraries containing amplifiers with response from weak to strong and with strength span of about 535 times are constructed. An amplifier and a CRISPRi system are combined to construct a turner, and gene expression inhibition with the strength span of about 200 times is achieved. The combination of the amplifier, the promoter library and the turner is called a multi-effect device, and abundant elements are provided for fine target gene expression. The combination of the trigger, the stabilizer and the multiple-effect device is called as a streptomycete multipath manual control system (SMARTS), and control tools of streptomycete are enriched.

The invention discloses a straw leavening agent suitable for a microfiltration membrane fermentation system and application of the straw leavening agent, and relates to the technical field of biology. The straw leavening agent comprises a microorganism combination. The microbial composition is prepared from bacillus subtilis (Bacillus subtilis) 1JN2, streptomyces globisporus (Streptomyces globisporus) XS2301 and myroides odortorius (Myroides odortorius) YW-1, and the microbial composition is prepared from bacillus subtilis (Bacillus subtilis) 1JN2, streptomyces globisporus (Streptomyces globisporus) XS2301 and myroides odortorius (Myroides odortorius) YW-1. The straw fermenting agent can improve the humification index in the straw composting process, remarkably shorten the composting period of crop straw and effectively reduce volatilization of irritant gas such as ammonia gas and hydrogen sulfide in the straw composting process. The straw leavening agent provided by the invention not only can efficiently treat crop straws, but also has the dual advantages of environmental protection and agricultural yield increase, and provides important technical support for agricultural sustainable development.

The invention discloses an efficient streptomyces mobaraensis CRISPR / Cas9 editing method, and belongs to the technical field of gene engineering. According to the method, a CRISPR / Cas9 system in streptomyces mobaraensis is successfully constructed and optimized, a related gene ssti of aerial hypha growth delay is selected as a reporter gene, a CRISPR / Cas9 editing system is optimized, an optimal promoter P19570 of Cas9 and the most appropriate sgRNA of a targeted ssti gene are obtained, and on the basis, a repair template with the length of 2kb is selected, so that the highest single gene editing efficiency reaches 90.8%. Furthermore, an array containing two gRNA expression cassettes is constructed, simultaneous knockout of ssti and sapB genes in smY2019 is realized, and the knockout efficiency is 6.25%.

An alditol oxidase and application thereof. The method uses D-glucose as a substrate to dock the alditol oxidase derived from Streptomyces coelicolor A3, and selects amino acid residues around an active center for saturation mutagenesis, and screens for alditol oxidase with D-glucose oxidizing activity by plate color development. An amino acid sequence of the alditol oxidase is shown in SEQ ID NO: 2 or SEQ ID NO: 4. The alditol oxidase has the activity of converting D-glucose to D-gluconic acid and D-glyceraldehyde to D-glyceric acid. By using the alditol oxidase, the conversion of D-glucose to pyruvic acid is realized by only three enzymes for the first time and does not depend on any coenzyme.

The invention discloses low-temperature salt-tolerant and alkali-tolerant streptomyces microflavus and application thereof, relates to the technical field of microorganisms, and is characterized in that the preservation number of the streptomyces microflavus is CCTCC (China Center for Type Culture Collection) M2023664. The streptomyces microflavus provided by the invention can well grow at the temperature of 10-30 DEG C. In a Congo red decoloration test, the ratio of the diameter (D) of a transparent circle to the diameter (d) of a bacterial colony is 3.6. The strain is applied to degradation of cellulose, has the advantages of degradation of cellulose at low temperature, high cellulase activity, salt resistance, alkali resistance and the like, and can be particularly used for cellulose degradation strain resources in low-temperature saline-alkali areas.

The invention belongs to the technical field of genetic engineering, and particularly relates to a method for increasing the yield of lincomycin by knocking out a streptomyces lincomycin pAgene0296 gene. The method comprises the following steps: knocking out a coding gene pAgene0296 of TetR / AcrR family repressor protein in streptomyces lincomycin, and fermenting the obtained mutant strain to produce lincomycin. Experimental results show that after the pAgene0296 gene of the streptomyces lincomycin is knocked out, the yield of the lincomycin is remarkably increased. The invention further provides a construction method of the pAgene0296 gene deletion type streptomyces lincoli mutant strain, according to the method, the coding gene of the streptomyces lincoli is directionally modified through a gene engineering technology, so that the pAgene0296 gene is knocked out, and the obtained pAgene0296 gene deletion type streptomyces lincoli mutant strain can be used for efficiently producing lincomycin or a product in the synthesis process of the lincomycin.

The invention provides a nitrification inhibitor for promoting root hair growth, a biostimulant and a preparation method and application of the biostimulant, the nitrification inhibitor comprises 3, 4-dimethylpyrazole and streptomyces fermentation liquor with the weight ratio of 1: (2.5-4.0), and the streptomyces fermentation liquor contains organic acid. The nitrification inhibitor provided by the embodiment of the invention can effectively promote the growth of root hair, further, the biostimulant prepared from the nitrification inhibitor can effectively induce up-regulation expression of auxin related genes ZmARF10, ZmYUC2, ZmYUC3, ZmYUC5, ZmPIN1 and ZmPIN9, the growth of crop root hair is promoted, the root activity of plants is greatly improved, the high-quality growth and yield of crops are promoted, and meanwhile, the biostimulant has good application prospects. The raw materials of the biostimulant are easy to obtain, the preparation method is simple, and industrialization is easy to realize.

The invention discloses a pretreatment method before aerobic composting of mixed traditional Chinese medicine residues, which comprises the following steps: crushing the mixed traditional Chinese medicine residues according to particle sizes by a flat grinding method, then carrying out high-temperature steam explosion, carrying out constant-temperature reaction on the obtained mixed traditional Chinese medicine residues and a deep eutectic solvent at 80-100 DEG C, and carrying out enzymolysis by using cellulase and ligninase at 50-60 DEG C to obtain the mixed traditional Chinese medicine residues. And finally, mixing with a compound fermentation inoculant which is streptomyces microflavus and bacillus subtilis, and reacting for 6-12 hours, so that through a multi-step comprehensive pretreatment process, active antibacterial factors are effectively eliminated, the opening degree of cellulose and the degradability of lignin are improved, the active antibacterial factors are effectively eliminated, and the degradation rate of lignin is improved. The degradation rate of cellulose and lignin in the traditional Chinese medicine residues is remarkably improved, and the degradation efficiency, the composting efficiency and the composting quality of the traditional Chinese medicine residues are remarkably improved.

The present invention discloses a compound microbial agent for alleviating continuous cropping obstacles of traditional Chinese medicine crops and its application. The compound microbial agent of the present invention contains Bacillus amyloliquefaciens Y34, Serratia marcescens Y35, Bacillus amyloliquefaciens Y40 and Streptomyces SD; it can be applied to improve soil, eliminate diseases of Polygonatum odoratum, Pogostemon cablin and Cynanchum stauntonii, and promote crop growth. The compound microbial agent of the present invention can reduce soil-borne diseases during the planting process of traditional Chinese medicine, alleviate continuous cropping obstacles of traditional Chinese medicine, improve the yield and quality of traditional Chinese medicine, and has important application value especially in the cultivation and production of traditional Chinese medicine crops such as Polygonatum odoratum and Pogostemon cablin.

The invention discloses a Streptomyces rochei D74 homogeneous polysaccharide and a preparation method and application thereof.The Streptomyces rochei D74 homogeneous polysaccharide is obtained by smashing Streptomyces rochei D74 mycelia obtained through liquid fermentation, then conducting water extraction, concentration, alcohol precipitation and deproteinization to obtain crude polysaccharide, and further conducting ion-exchange chromatography, dialysis and gel column chromatography purification to obtain the Streptomyces rochei D74 homogeneous polysaccharide. The method is simple and efficient, and the obtained streptomyces homogeneous polysaccharide remarkably inhibits proliferation of human gastric cancer cells SGC-7901 by inducing tumor cell apoptosis; meanwhile, immune cells RAW264.7 can be activated, the phagocytic ability and the nitric oxide (NO) release ability of the immune cells RAW264.7 can be improved, and the polysaccharide can become a potential polysaccharide drug for treating human liver cancer.

This invention relates to the fields of bioengineering and fermentation engineering, and discloses a genetically engineered *Streptomyces albopictus* strain that produces high levels of ε-polylysine and its fermentation production process. This genetically engineered strain uses *Streptomyces albopictus* strains acclimated to ε-polylysine tolerance as a chassis, and its genome integrates... ppc and dapf Through expression box, heterogeneous asd Expression cassettes, containing secretory signal peptides lysp Fusion expression cassettes and D404A / K499A point mutations pls Expression cassette. The fermentation process employs a two-stage pH control strategy, maintaining a neutral environment during the cell growth phase and adjusting to an acidic environment during product synthesis. This invention improves ε-polylysine yield through a synergistic strategy of enhancing precursor supply, constructing efflux detoxification channels, and increasing synthase activity; combined with an acidic fermentation process, it effectively inhibits product degradation, yielding a high-molecular-weight and highly uniform target product suitable for industrial production.

The invention belongs to the technical field of medicinal chemistry, and particularly relates to a series of 15-membered ring quadruple lactone derivatives with neoantimycin mother nucleus structures, a preparation method of the 15-membered ring quadruple lactone derivatives and application of the 15-membered ring quadruple lactone derivatives in antitumor drugs, wherein the 15-membered ring quadruple lactone derivatives are generated by feeding chemical precursors in the fermentation process of streptomyces S.conglobatusATCC 31005. The compound is a fifteen-membered ring quadruple lactone derivative, the C-2 position of the derivative is connected with a carbonyl group or a hydroxyl group, the C-4 position and the C-9 position are substituted by aliphatic chains with different lengths, and the C-6 position of the derivative is connected with a benzoic acid group containing F atoms or hydroxyl groups. The compounds have obvious tumor cell proliferation inhibition activity on colorectal cancer cells and lung cancer cells, the inhibition activity is equivalent to that of a control drug oxaliplatin, and the compounds have weak toxicity on non-cancer cell strains. A novel lead compound is provided for research and development of novel antitumor drugs, and the compound is expected to be developed into a tumor inhibitor.

The invention relates to a feeding method for improving the fermentation unit of tylosin, which comprises the following steps: inoculating a seed solution into a fermentation tank filled with a liquid fermentation culture medium by taking streptomyces fradiae as a fermentation strain, producing tylosin by adopting a fed-batch fermentation process, fermenting for 12 hours, adding a sodium malonate solution, reacting for 2 hours, adding a VB12 solution, fermenting for 30-130 hours, and then adding a VB12 solution to react for 2 hours. And supplementing the mecobalamin solution every 12 hours. By adopting the material supplementing process, the fermentation unit of tylosin can be effectively increased by 2-4% or above, and the material supplementing process is suitable for industrial production.

The invention discloses a method for constructing a pressed butterfly mycin glycoside gene engineering strain by using glycosyl transferase and application of the pressed butterfly mycin glycoside gene engineering strain. A strong promoter kasOp * is introduced to the upstream of a glucoside transferase gene pie28 of Streptomyces psammoticus SCSIO NS126, the nucleotide sequence of the strong promoter kasOp * is as shown in SEQ ID NO. 1, and the nucleotide sequence of the glucoside transferase pie28 is as shown in SEQ ID NO. 2. The invention also discloses a preparation method of the Streptomyces psammoticus SCSIO NS126, and the preparation method of the Streptomyces psammoticus SCSIO NS126 comprises the following steps of: introducing the strong promoter kasOp * into the upstream of the glucoside transferase gene pie28. The streptomyces gene engineering strain Streptomyces sp. SK-kas28 constructed by the invention can efficiently express the chalcone glycoside, is widely applied to fermentation production of the chalcone glycoside, and is used for research and development of new drugs for treating kidney diseases and the like.

The present invention belongs to the technical field of maize disease control methods, and particularly relates to a biological bactericide for controlling maize ear rot, its preparation method and application. The biological bactericide comprises a water dispersible granule carrier mixture and a biocontrol bacteria culture solution, and the mass ratio of the water dispersible granule carrier mixture to the biocontrol bacteria culture solution is 2-3:1; the water dispersible granule carrier mixture comprises diatomaceous earth, chitosan and sucrose, and the mass ratio of diatomaceous earth, chitosan and sucrose is 1-2:1-2:1; the biocontrol bacteria culture solution is obtained by mixing fermentation broths of Bacillus subtilis, Streptomyces microflavus, Paenibacillus polymyxa and Bacillus velezensis. The biocontrol bactericide provided by the present invention has a very significant effect on controlling maize ear rot caused by Fusarium verticillioides, has the potential to be used as a new biological pesticide for controlling ear rot caused by Fusarium verticillioides in maize production, and has the value of further research.

The invention discloses a preparation method and application of a novel heterocyclic isomer sourced from streptomyces. The heterocyclic isomer is separated from a fermentation culture of streptomyces and has a remarkable antibacterial effect.

The present invention provides potent antibiofilm and antifouling compounds albofungin and derivatives thereof isolated from Streptomyces chrestomyceticus BCC 24770, the construction of high-yield strains for albofungin production, and application of albofungin-based copolymer coatings to object or surfaces, particularly to inhibit fouling by marine organisms using hydrolysable and degradable antifouling coatings incorporated with albofungins.

The present application relates to a kind of polyantimycin production strain and its application, the polyantimycin production strain is Streptomyces Streptomyces Sp.MKL-0403, the polyantimycin production strain not only has polyantimycin production activity, and its fermentation product has higher bacteriostatic activity and more extensive bacteriostatic range.

The present application relates to the technical field of biological medicine, in particular to streptomyces AH1901-2 and application thereof, preparation method and application of naphthymycin compounds, and belongs to the field of biological medicine. The streptomyces AH1901-2 is isolated from rhizosphere soil of Ligularia sibirica in Tianshan region (5.8°E, 28.0°N) in Xinjiang, and naphthymycin compounds are isolated and purified from liquid fermentation liquor of the streptomyces AH1901-2, and subsequent studies show that the naphthymycin compounds have obvious inhibitory effect on NO production of mouse mononuclear macrophages, and can be used for preparing anti-inflammatory drugs and treating inflammation.

The present invention provides a method for increasing the yield of lincomycin by modifying the SLCG_2951 gene of Streptomyces lincomycin, knocking out the SLCG_2951 gene of Streptomyces lincomycin by genetic engineering, obtaining a high-yield strain of lincomycin, and fermenting and producing lincomycin A with the obtained strain; wherein the nucleotide sequence of the SLCG_2951 gene is shown in SEQ ID NO.1. The present invention also provides an application of the above method. The present invention screens the SLCG_2951 gene that affects the mycelium morphology, knocks out the SLCG_2951 gene on the genome of Streptomyces lincomycin by genetic engineering, and can delay the mycelium breakage of Streptomyces lincomycin A134 on a fermentation tank, thereby increasing the yield of lincomycin A, and providing technical support for increasing the yield of lincomycin in industrial production.