226 results about "Streptomyces thermodiastaticus" patented technology

The invention provides a multifunctional active organic fertilizer and a preparation method thereof, relating to an organic fertilizer containing bacteria, fungi and spores, particularly an active organic fertilizer with functions of soil restoration and disease/pest resistance. The invention aims to provide a multifunctional active organic fertilizer capable of restoring soil pollution and improving product quality and a preparation method thereof. The multifunctional active organic fertilizer is composed of three layers from inside to outside, wherein the innermost layer is composed of a component A of which the main effective components are Bacillus subtilis and Paecilomyces lilacinus; the middle layer is composed of a component B and a component C containing multiple rich nutritive elements; and the outermost layer is composed of a component D of which the main effective components are Streptomyces vinaceus-drappus and Gliocladium roseum. The multifunctional active organic fertilizer can passivate heavy metals in soil, activate the soil, improve the microecological environment of the rhizosphere, degrade the chemical pesticides and improve the crop quality. The multifunctional active organic fertilizer is used in the field of agricultural planting.

The invention relates to an antibiotic streptomyces, fermentation product of which can inhibit broad spectrum fungus and protozoan, and field experiment can control crop fungus plant disease and root-knot nematode. The antibiotic streptomyces has good effect, no pollution nuisance and no residue. The antifungal agent has extremely low hazardness for the people and good performance and development prospect for medical and agricultural use. The invention is named as SIM001 antibiotic streptomyces which is Streptomyces antibioticus subspecies xi an, and is preserved as patent in the depositary institution appointed by patent office of state intellectual property office; the preservation data is April 14th, 2005, the name of the depositary institution is CGMCC, and the number of preservation is CGMCC No.1349. The whole cell hydrolysate of the antibiotic streptomyces contains L, L-DAP (Diaminopimelic acid) and no characteristic glucide; the cytoderm belongs to I type and glucide type C; one of the main antibacterial materials is polyene macrocyclic ketolide which belongs to the broad spectrum antifungal substance.

The invention relates to the technical field of gene recombination, and particularly relates to streptomycete generating 5-keto-milbemycins and a method for producing 5-keto-milbemycins. The invention provides a recombinant vector for knocking out the milF gene of streptomycete, including the homologous fragment of the milF gene, wherein the sequence of the fragment is obtained by losing 105-767 nucleotides from the nucleotides from the sites 158-924 in the nucleotide sequence shown by SEQ ID No.2. The invention also provides an establishment method of the recombinant vector, a method for knocking out the milF gene in streptomycete by use of the recombinant vector and streptomycete without milF gene. The streptomycete can be directly fermented to generate 5-keto-milbemycins, so that the synthesis process of milbemycin oxime is simplified, and the pollution caused by the traditional chemical synthesis method is avoided.

The invention discloses a preparation method of a high temperature-resistant and salt-resistant kitchen waste decomposing compound bacteria agent. The preparation method comprises the following steps:respectively performing seed culture on streptomycete, bacillus subtilis, bacillus amyloliquefaciens and saccharomyces cerevisiae, so that a streptomycete mycelium pellet suspension, a bacillus subtilis bacteria solution, a bacillus amyloliquefaciens bacteria solution and a saccharomyces cerevisiae bacteria solution are respectively and correspondingly obtained; inoculating the streptomycete mycelium pellet suspension to a solid fermentation material for culture to obtain solid decomposing bacteria; mixing the other three bacteria solutions to obtain a compound bacteria solution; stirring anduniformly mixing the compound bacteria solution and the solid decomposing bacteria according to the proportion of 9-11 ml/100g, then drying at a low temperature, and finally adding an enzyme preparation for mixing to obtain the decomposing compound bacteria agent. The compound bacteria agent is used for decomposing kitchen wastes.

The invention discloses a special polyelement microorganism fertilizer for preventing yam anthracnose and a preparation method of the special polyelement microorganism fertilizer. The special polyelement microorganism fertilizer comprises components of raw materials in parts by weight as follows: 2-4 parts of bacillus subtilis, 1-3 parts of frankia, 3-5 parts of streptomycete, 2-4 parts of nystatin, 50-60 parts of urea, 25-30 parts of ammonium phosphate, 15-20 parts of borax, Chinese herbal medicinal ingredients and the like. The special polyelement microorganism fertilizer can reduce the morbidity of the anthracnose, increase the yield of yams, and improve the soil condition.

The invention provides a streptomycesvinaceus-drappus microbial inoculum. The streptomycesvinaceus-drappus microbial inoculum uses an active bacteria culture prepared by liquid submerged fermentation or solid fermentation of an active strain of streptomycesvinaceus-drappus, of which the storage number is CGMCC No. 2664, as an active ingredient. A tentative name of the streptomycesvinaceus-drappus microbial inoculum is S506, and the streptomycesvinaceus-drappus microbial inoculum was submitted to the CGMCC for storage on 11st September, 2008. The preparation method comprises the following steps of: A, activation of the strain; B, preparation of liquid seeds of the streptomycesvinaceus-drappus; and C, liquid submerged fermentation of the streptomycesvinaceus-drappus. The invention also provides a novel process of 'liquid seed preparation and solid fermentation' which is favorable for the growth of the streptomycesvinaceus-drappus, and the novel process meets the technical requirements on high effective viable count, high spore production rate, light contamination of crossbred bacteria and short fermentation period. When the active streptomycesvinaceus-drappus is applied to the roots of vegetables, various root diseases, such as blight, root rot and root knot nematode disease, of vegetables can be effectively prevented and controlled, and the streptomycesvinaceus-drappus microbial inoculum has obvious effects of enforcing growth of root systems and degrading autopoisonous matters of root systems, so that the streptomycesvinaceus-drappus microbial inoculum has a wide application prospect in effectively solving the problem of continuous cropping obstacles which are commonly happed to vegetables, fruit trees, medicinal materials and the like.

A synthetic method and an extraction process of blue natural dye belong to the field of biological engineering. The purpose of the invention is to provide the synthetic method and the extraction process by using biological materials to synthesize non-toxic and environmental-friendly blue natural dye. The present invention uses purified brown chromogenic streptomycete genomic DNA as a template, uses a primer C1: 5'-aaTTAATTAAGGAGGAGCCCATatgagcgtagagaccatccc-3' and a primer C2: 5'-aaGCTAGCAAGCTTtcagtagttgggcgtcttgc-3' to was amplify indigoidine synthase gene SC-indC by a polymerase chain reaction, wherein the amplified SC-indC gene sequence is SEQ ID NO: 1. The method and the extraction process using engineering bacteria to synthesize blue natural dye indigoidine are high in yield, not restricted by sources of raw materials, easy to control the process, and low in production costs.

The invention discloses a livestock and poultry manure decomposing inoculant, a use method thereof and a biological organic fertilizer prepared by using the livestock and poultry manure decomposing inoculant and fermenting through a special process. The livestock and poultry manure decomposing inoculant is prepared by compounding the following materials in parts by weight: 0.5-2 parts of bacillus subtilis, 0.5-2 parts of streptomyces microflavus, 0.5-2 parts of trichoderma longibrachiatum and 0.5-2 parts of bacillus mucilagimosus krassilm, wherein the effective viable count is 6*10<8> per gram. The livestock and poultry manure decomposing inoculant disclosed by the invention has multiple functions, can be used for decomposing the livestock and poultry manure and removing the odor and can resist the fusarium oxysporum and increase the yield after being applied to crops. When the livestock and poultry manure decomposing inoculant is applied to treating the livestock and poultry manure, the bacterial count, C/N ratio and fermentation period condition are all better than those of the comparison of an unknown inoculant. The biological organic fertilizer is prepared by adding 1 kilogram of the livestock and poultry manure decomposing inoculant to every 5 squares of the livestock and poultry manure and then fermenting through the special process. The livestock and poultry manure is formed by mixing the following materials in parts by weight: 40-60 parts of chicken manure, 20-40 parts of cattle manure, 5-15 parts of pig manure and 5-15 parts of lignite. The biological organic fertilizer disclosed by the invention is applied to scallion planting, achieves the disease control efficiency up to 56% and increases the yield per mu by 20.5%.

The invention discloses a streptomycete constitutive promoter and applications thereof, and provides an DNA fragment. The DNA fragment is any one selected from the following DNA molecules in 1)-3): 1) an DNA molecule represented by the sequence 1 in a sequence table; 2) an DNA molecule with promoter functions and hybrid with the DNA molecule shown in 1) under strict conditions; 3) an DNA molecule with more than 90% of homology with the DNA molecule shown in 1) and with promoter functions. The experiments shows that a strong constitutive promoter kasO*p is screened out, and the promoter and the erythromycin promoter ermE*p are compared in representation of transcriptional level and expression level in streptomyces coelicolor, streptomyces avermitilis and streptomyces venezuelae, and the results show that the modified promoter kasO*p is better than the the erythromycin promoter ermE*p.

The invention discloses a kitchen waste composite microbial degradation agent as well as a preparation method and application thereof. The degradation agent is prepared from Bacillus subtilis, Streptomyces thermodiastaticus, Bacillus stearothermophilus, Lactobacillus acidophilus, Saccharomyces cerevisiae, Enteromorpha protease, Methylobacterium radiora, Pantoea dispersa, Pseudomonas oryzihabitans,Citrobacter freundii and Staphylococcus cohnii. The mixed strain accounts for 15-25% of the total mass of the degradation agent, the mass percentage of water in the degradation agent is 10-20%, the pH value of the degradation agent is 5.5-8.5, and each gram of the degradation agent contains 106-109 viable bacteria. The degradation agent has the beneficial effects that: kitchen waste containing various substances such as vegetables, grains and meat can be effectively degraded, the activity of microorganisms in the degrading agent is high, the degrading rate reaches 80% or above, the degradingrate is high, the use amount is small when the kitchen waste is treated, generated peculiar smells are small, the cost is low, use is convenient, toxic and harmful substances are not generated, and safety, reliability and environment friendliness are achieved.

The invention discloses a method for producing a seedling raising substrate by using cotton straw and a substrate prepared with the same. The method comprises the following steps of: smashing cotton straw into sections of 10-50 millimeters; adding water till the water content is 60-80 percent according to mass percentage; adding 2-3kg of urea, 0.5-1kg of calcium superphosphate, 1-2kg of gypsum and 50-100g of fermentation inoculum into each cubic meter of substances obtained in the last step according to the volume ratio; mixing uniformly; and sealing, piling and fermenting at the air temperature of 15-40 DEG C, wherein the adopted compound fermentation inoculum is prepared by mixing pseudomonas aeruginosa, streptomyces albus and flavobacterium in the ratio of 2:3:1, and can be used by fermenting, airing in a scattered way and drying. In the method, a main raw material is derived from a large amount of waste cotton straw generated during the production of cotton, the raw material source is rich, a preparation process is simple, the aim of utilizing resources is fulfilled, and resource waste of cotton straw caused by direct smashing and returning to field can be reduced; and the method has a wide application value.

The invention provides a method for producing tacrolimus by fermentation, belonging to the field of biomedicines. According to the method of producing tacrolimus by fermentation, parameters such as a strain-activating bevel culture medium component, a liquid culture medium component, seed liquid inoculum size and a fermentation medium component are optimized to maintain the growing activity and the fermenting activity of streptomycete, so that the production of tacrolimus is improved. In the fermenting process, piperidine acid is added into the fermentation medium and the adding manner such as adding time and addition is optimized, so that the feedback inhibition of products is reduced, and meanwhile, the output of the final product tacrolimus is increased, so that the final output of tacrolimus can reach 385 microgram/mL.

A solid fermenting process of xanthostreptomycete includes such steps as proportionally mixing soybean cake powder, peanut shell, corn flour, millet flour, wheat bran, and lime and water, high-pressure sterilizing at 128-135 deg.C for 60-90 min, inoculating seeds, and fermenting for 144 hr. Its apparatus is composed of dual-screw stirrer, disinfecting stainless steel tray, sterilizing carriage, high-pressure sterilizing pot, collectnig hopper, belt conveyer, humidify meter, fermenting tray and fermenting case.

The invention discloses a characteristic fertilizer for cigarette and a production method thereof. At present, it is common to increasingly use organic fertilizers for improvement of soil and increase the content of flavor matters in tobacco leaves in modern tobacco agriculture. The invention provides the characteristic fertilizer for cigarette capable of effectively promoting formation of dominant microflora at the root of flue-cured tobacco and the production method thereof. The method comprises the following steps: uniformly mixing oil cake, bacillus subtilis, bacillus licheniformis, sporotrichum thermophile and trichoderma viride with stirring and standing an obtained mixture for fermentation; adding phosphate dissolving bacillus megaterium and streptomyces jingyangensis and continuing fermentation; adding potassium releasing bacillus mucilaginosus and streptomyces coerulescens and continuing fermentation until the temperature of the mixture drops to normal temperature; carrying out granulation; preparing inorganic particles; carrying out mixing. The production method for the characteristic fertilizer for cigarette enables larva of mosquitoes and flies propagated in the process of maturing of organic materials to be killed and environment cleanliness of a fermentation place to be improved, and has the advantages of a simple production process and a short production period.

The invention belongs to the organic fertilizer technical field, in particular relating to a soil improvement type composite biological organic liquid fertilizer. The soil improvement type composite biological organic liquid fertilizer is prepared by combination of bifidobacterium, lactobacillus bulgaricus, lactobacillus casei, saccharomyces cerevisiae, bacillus subtilis, streptomyces hygroscopicus and so on. The compositions are all profitable strains sieved from soil, are cultured and multiplied by application of the biotechnology, added with organic substances and nutritive elements, and processed into the novel composite biological organic liquid fertilizer by means of chelating, and are favorable for recovering the ecosystem of soil organisms. Therefore, the product of the soil improvement type composite biological organic liquid fertilizer not only has the function of fertilization but also can improve the physical property and the biological property of the soil and have the function of improving the soil, and can be widely used for various crops.

The invention provides a tetrahydroquinoline alkaloid Malaysiensin with immunosuppression activity and a production method and application of the tetrahydroquinoline alkaloid Malaysiensin with the immunosuppression activity. The compound is obtained by conducting SFM solid medium fermentation on Streptomyces sp.DSM 4137 and separating and purifying a fermentation product. The compound can significantly inhibit ConA-induced proliferation of T cells, an immunosuppression effect of the compound is equivalent to that of an immunosuppressor cyclosporin A which is clinically and widely used, and action mechanism studies show that Malaysiensin exerts the immunosuppression activity for a targeted NFAT signal path, is an efficient immunosuppressor, and can be used for producing immunosuppressive medicines for organ transplantation and autoimmune diseases.

The invention relates to biocontrol streptomycete. The preservation number of the strain of the biocontrol streptomycete is CGMCC No.18658. During cultivation, the biocontrol streptomycete is inoculated to a fermentation medium for fermentation cultivation. The strain or a biocontrol product prepared through the strain can be used for plant pathogen preventing and treating. The biocontrol streptomycete or a biocontrol preparation prepared through the biocontrol streptomycete has the advantages of good antibacterial effect, broad antimicrobial spectrum, low toxicity, no residue, environmental friendliness, broad development prospect and the like.

The invention discloses a nosiheptide biosynthetic regulatory protein NosP specific binding sequence and an influence thereof upon nosiheptide biosynthesis effectiveness. The invention comprises the following contents: recombinant plasmids comprising specific affinity purification tags and truncated NosP gene are established; a NosP protein pure product is prepared through Escherichia coli heterologous expression and an affinity chromatography method; and with EMSA and DNase I footprinting assay technologies, the NosP specific binding sequence in a streptomyces actuosus nosiheptide biosynthetic gene cluster is found and verified. On this basis, through nosiheptide biosynthetic pathway renovation, nosiheptide biosynthesis effectiveness is improved by 150%, such that an application prospect is good.

The invention discloses a special compound microorganism fertilizer for preventing yam anthracnose and a preparation method of the special compound microorganism fertilizer. The special compound microorganism fertilizer for preventing the yam anthracnose comprises components in parts by weight as follows: 2-4 parts of bacillus subtilis, 1-3 parts of frankia, 3-5 parts of streptomycete, 2-4 parts of nystatin, 50-60 parts of urea, 25-30 parts of ammonium phosphate, 15-20 parts of borax, 10-15 parts of ferrous sulfate, 8-10 parts of manganese sulfate, 10-12 parts of zinc sulfate, 20-24 parts of calcium superphosphate, 80-100 parts of peanut cakes, 70-80 parts of a soybean meal, 100-120 parts of maize straw, 90-100 parts of expansion chicken manure, 40-50 parts of waste of a chicken slaughter house, 0.2-0.4 part of multi-effect triazole and 0.4-0.6 part of gibberellin. The special compound microorganism fertilizer can reduce the morbidity of the anthracnose, increase the yield of yams, and improve the soil condition.

The invention provides a high-yield tacrolimus streptomycete mutant strain. The mutant strain is (Streptomyces tsukubaensis)FIM-07-23, and preserved at the common microorganism center of China Committee for Culture Collection of Microorganisms on January 7th, 2019, the address is No.3, courtyard 1, the western Beichen road, Chaoyang District of Beijing, and the preservation number is CGMCC No.17137; the (Streptomyces tsukubaensis)FIM-07-23 is applied into fermentation production of tacrolimus. The (Streptomyces tsukubaensis)FIM-07-23 can ferment high-yield tacrolimus, the yield of tacrolimus is greatly increased, and the genetic stability is good.

The invention discloses a composite enzymolysis reagent for keratin enzymolysis and an application method thereof. The composite enzymolysis reagent comprises streptomycete cutin protease, yeast enzyme and a reducing agent. The application method comprises the following steps: firstly, performing ungrease treatment on a natural raw material with cornifin, a yeast enzyme and a reducing agent; adding the natural raw material with the cornifin after ungrease treatment in the step 1) into a combined enzymolysis agent liquid, pretreating for 15-25 minutes at the room temperature without oxygen, further adjusting the pH value of the combined enzymolysis agent liquid to be 7.0-7.5, controlling the temperature to be 50-60 DEG C, and performing enzymolysis for 12-24 hours, thereby obtaining a keratinase liquid; decoloring and sterilizing the keratinase liquid; and performing vacuum drying, thereby obtaining protein powder. The composite enzymolysis reagent is rapid in enzymolysis and high in keratin degradation rate, and generation of toxic substances can be effectively prevented, so that the prepared protein powder is safe and non-toxic and high in nutrition component.

The invention relates to the technical field of plant biocontrol preparations, specifically to a biocontrol preparation for disease prevention of tobacco and a preparation method thereof. The invention aims to provide a biocontrol preparation for disease prevention of tobacco and a preparation method thereof. The preparation method comprises the following steps: evenly mixing a mixed medium freeze-dried powder, sugarcane powder and plant ash according to a certain ratio so as to obtain pulvis of the biocontrol preparation for disease prevention of tobacco; mixing the mixed medium freeze-driedpowder and some organic matter and dissolving by adding water so as to obtain the biocontrol preparation for disease prevention of tobacco. The mixed medium freeze-dried powder comprises medium freeze-dried powder of bacillus subtilis, Trichoderma harzianum and Streptomyces microflavus. The biocontrol preparation for disease prevention of tobacco can control some common but hazardous diseases of tobacco.

The invention discloses a method for biosynthesizing virginiamycin by streptomycete, which comprises the following steps of: preparing a slope with spores of virginiamycin; inoculating the slope spores into a culture medium to obtain a seed solution; inoculating the seed solution into a fermentation culture medium for fermentation culture, adding a precursor substance at the early stage of fermentation so as to increase the yield of virginiamycin; and further processing the fermentation solution after fermentation to obtain virginiamycin crystals. The method disclosed by the invention is simple and easy to implement, is stable in the fermentation result and low in production cost. The fermentation result is improved compared with the traditional process, and the highest yield of virginiamycin can reach 3500 mu g/ml. Virginiamycin is polypeptide antibiotic containing lactonic rings, has the characteristics of no toxicity, no accumulation, easiness in degradation, and no drug resistance, and is a new animal feed additive.

The invention discloses a composite microbial agent for a coal gangue artificial ecological matrix, which comprises five actinomycetes, streptomyces thermoviolaceus, streptomyces thermodiastaticus, streptomyces thermocarboxydus, streptomyces albidoflavus and streptomyces thermovulgaris, and one bacterium is pseudomonas sp. and one mould is penicillium oxalicum. The preparation process of the composite microbial agent comprises the following four steps: activating strains, preparing a seed solution, preparing a liquid composite microbial inoculum and preparing a solid microbial inoculum. According to the technology, the problems that microorganisms in the coal gangue are lacked, and nutrients are not beneficial to plant absorption and utilization are solved, a new way is provided for resource utilization of the coal gangue and landscaping waste, and the preparation has wide application prospects in the aspects of treatment, resource utilization and the like of the coal gangue.

The invention discloses engineering bacillus subtilis capable of expressing phospholipase D, and belongs to the technical field of gene engineering. The engineering bacillus subtilis capable of expressing the phospholipase D is constructed by taking bacillus subtilis (WB600) as an expression host and taking a phospholipase D encoding gene (PLD) from streptomyces racemochromogenes as a target gene.The activity of the phospholipase D produced by the engineering bacteria can be up to 5.916 U/mL and has an extremely high application value in the field of medicines, food and health care products;meanwhile, the phospholipase D adopted by the engineering bacteria is actinomyces-derived endogenous phospholipase D, which has high reaction activity, high phosphatidyl substrate selectivity and highorganic matter stability; furthermore, a catalysis structure is the most compact; the phospholipase D is more suitable for efficient synthesis of phospholipid and a phospholipid derivative in the industrial production.