230 results about "Transferase Gene" patented technology

The invention discloses an attenuated salmonella typhimurium EN-VNP (Enhanced Natriuretic Peptide) strain as well as a preparation method and application of the attenuated salmonella typhimurium EN-VNP strain. Comprising the following steps: on the basis of a salmonella VNP20009 strain, flagellin genes fliC and fljB, a phosphoethanolamine transferase gene eptA, a 4-amino-4-deoxy-L-arabinose transferase gene arnT and phosphotransferase yeiU are sequentially knocked out or silenced, and a strain is formed after a phosphotransferase gene lpxE is knocked in or overexpressed, so that the attenuated salmonella typhimurium EN-VNP strain is prepared. According to the attenuated salmonella typhimurium EN-VNP with low toxicity and the T cell adjuvant effect and an EN-OMV antigen delivery system prepared from the attenuated salmonella typhimurium EN-VNP, an improved strain does not contain flagellin, an LPS molecular structure is changed, the attenuated salmonella typhimurium EN-VNP has the low toxicity and the T cell adjuvant effect, and normal multiplication culture can be achieved.

The invention discloses a genetically engineered bacterium for producing uridine diphosphate glucose as well as a construction method and application of the genetically engineered bacterium, and belongs to the technical field of genetic engineering. The following genes are overexpressed in the genetically engineered bacterium: a pyrimidine nucleoside operon gene, a carbamyl phosphate synthetase gene, an aspartate carbamyl transaminase gene, an orotic acid phosphoribose transferase gene, a phosphoribose pyrophosphate synthase gene, a uridylate kinase gene and a glucophosphate mutase gene. A uridine triphosphate glucose-1-phosphate uridine group transferase gene; the genetically engineered bacterium does not express the following genes: a purine nucleotide repressor protein gene, a deoxyribonucleoside triphosphate reductase gene, a nucleoside triphosphate pyrophosphohydrolase gene, a cytidine triphosphate synthase gene, a pyrimidine nucleotidase gene, a nucleotidase gene, an ornithine carbamoyltransferase gene, a UDPG dehydrogenase gene and a lacI repressor protein gene. The genetically engineered bacterium can be used for efficiently synthesizing UDPG by taking glucose as a substrate.

The invention belongs to the technical field of plant genetic engineering, and particularly relates to application of a centella asiatica glycosyl transferase gene in catalyzing glycosylation of asiatic acid and madecassic acid. The nucleotide sequence of the gene is shown as SEQ ID No.1, and the protein coded by the gene can catalyze the C-28 site of asiatic acid and madecassic acid to perform glucose group modification so as to generate asiatic acid monoglucoside and madecassic acid monoglucoside, and preferentially catalyzes madecassic acid. By constructing a metabolic regulation network, the genes are widely and accurately screened, and the glycosylation process of catalyzing asiatic acid and madecassic acid is analyzed; an important reference basis is provided for improving the glycosylation efficiency of asiatic acid and madecassic acid and the yield of asiaticoside and madecassoside in the in-vitro synthesis and industrial production process of the centella asiatica triterpenoid saponin, and an important theoretical support is provided for large-scale production of the centella asiatica active saponin.

The invention relates to a recombinant thallus, a construction method of the recombinant thallus and application of the recombinant thallus in production of xanthoxylin WGX-50, and relates to the technical field of bioengineering. A recombinant plasmid containing a phenylalanine ammonialyase gene PAL with a nucleotide sequence as shown in SEQ ID NO.2 and a cinnamic acid coenzyme A ligase gene CNL with a nucleotide sequence as shown in SEQ ID NO.1 and a recombinant plasmid containing a tyramine N-hydroxycinnamoyl transferase gene THT with a nucleotide sequence as shown in SEQ ID NO.3 are introduced into the recombinant plasmid. A phenylalanine ammonialyase gene PAL, a cinnamic acid coenzyme A ligase gene CNL and a tyramine N-hydroxycinnamoyl transferase gene THT are overexpressed in escherichia coli, so that a biological synthesis path of xanthoxylin WGX-50 is formed by taking 3, 4-dioxomethyl phenylethylamine as a substrate for the first time, a foundation is laid for large-scale industrial production, and the method has a wide application prospect. The method has important economic value and social benefit.

The invention provides application of acidophilous glycosyltransferase in salidroside production, and belongs to the technical field of biological engineering. The problem of producing salidroside under the acidic condition is solved. Comprising an application of acidophilous glycosyl transferase with an amino acid sequence as shown in SEQ ID NO.1 in salidroside production under an acidic condition and an acidophilous escherichia coli engineering strain for producing salidroside. The escherichia coli engineering strain overexpresses a mutant 3-deoxy-D-arabinoheptulose-7-phosphate (DAHP) synthetase gene aroGfbr, a cyclohexadiene dehydrogenase gene tyrC, a glucose phosphate mutant enzyme gene pgm and a UDP-glucose pyrophosphorylase galU, overexpresses a phenylpyruvate decarboxylase gene ARO10 derived from saccharomyces cerevisiae, and can be used for producing a mutant 3-deoxy-D-arabinoheptulose-7-phosphate mutant enzyme. The kit comprises an ethanol dehydrogenase gene ADH6 and a glycosyl transferase gene LrUGT85AF8. The method is mainly used for producing salidroside under an acidic condition.

The invention relates to a methyltransferase gene for catalyzing methylation of resveratrol and application of the methyltransferase gene, and belongs to the technical field of biology. The invention provides a gene of methyltransferase capable of catalyzing resveratrol to generate pinostilbene and pterostilbene. The gene of the methyltransferase is a gene YC26, a gene SS15 or a gene HS97-5. The methyltransferase gene provided by the invention has the advantages of high substrate conversion rate, rich products, high activity and the like, the conversion rate of resveratrol can be increased and synthesis of pterostilbene can be promoted when the methyltransferase gene is combined with other methyltransferase genes to express in different strains, additional addition of expensive methyl donors is avoided, and the production cost is reduced. Only a small amount of substrate resveratrol needs to be added or no substrate resveratrol is added.

The invention relates to the technical field of gene engineering, in particular to a glycosyl transferase mutant and application thereof in synthesis of rebaudioside M. Wild type glycosyl transferase is modified through a directed evolution theory to obtain the glycosyl transferase mutant, so that the efficiency of synthesizing rebaudioside D through reaction of rebaudioside A is effectively improved. Besides, recombinant bacteria capable of simultaneously expressing the glycosyl transferase mutant, a second glycosyl transferase gene and a sucrose synthase gene are constructed, a wet cell obtained by induced culture of the recombinant bacteria or a crude enzyme liquid extracted by crushing the wet cell is used as a catalyst, rebaudioside A is used as a substrate, sucrose is used as an auxiliary substrate, and the rebaudioside M is catalytically synthesized by a one-pot method. According to the present invention, the complete catalysis of 100 g / L of rebaudioside A to produce rebaudioside M only needs 24 h, the conversion rate reaches 97.5%, the yield of rebaudioside M reaches 130 g / L, the raw material conversion rate and the yield of rebaudioside M are effectively improved, and the synthesis time is shortened.

The invention belongs to the technical field of biology, and particularly relates to recombinant escherichia coli for producing melonic acid and application of the recombinant escherichia coli. Escherichia coli MG1655 is used as a host cell, and HMG-CoA synthetase MvaS gene and HMG-CoA reductase MvaE gene derived from lactobacillus casei, acetyl-coenzyme A acetyltransferase atoB gene derived from escherichia coli, phosphoketoenzyme fxpk gene derived from bifidobacterium adolescentis and fructose-1, 3-D-glucanase derived from escherichia coli are synthesized into an HMG-CoA synthetase MvaS gene and HMG-CoA reductase MvaE gene derived from lactobacillus casei. And transferring the 2, 6-diphosphatase fbp gene into Escherichia coli host cells to obtain the gene. The recombinant Escherichia coli constructed by the invention can be used for synthesis of the melonic acid, the yield of the melonic acid can reach 87.86 g / L after the recombinant Escherichia coli takes glucose as a substrate and is fermented for 48 hours in a 5L fermentation tank at 37 DEG C, and the recombinant Escherichia coli has the advantages of short fermentation time and high yield and meets the requirements of industrial production.

The invention discloses gene-simplified bacillus subtilis for producing surfactin and application of the gene-simplified bacillus subtilis, and belongs to the technical field of gene engineering. According to the method disclosed by the invention, the variety of non-essential genes is determined by analyzing and identifying the bad performance influencing the fermentation production of the surfactin by the bacillus subtilis. Therefore, on the basis of the bacillus subtilis 168, a phosphopantetheinyl transferase (sfp) gene is firstly integrated, so that the surfactin production capacity of host bacteria is recovered; then, genes (clusters) in five aspects of a biological membrane synthesis pathway, a metabolic by-product pathway, spore generation, a protease system and flagellum generation are simplified. After the strain EX10 is simplified, the fermentation performance is optimized, and the fermentation yield reaches up to 11.2 g / L, which is 2.1 times higher than that of the original strain. Therefore, the genome-simplified strain has a good application prospect as a chassis cell for industrially and efficiently producing surfactin.

The invention belongs to the technical field of genetic engineering, and discloses a safe recombinant bacillus subtilis for synthesizing lactoyl-N-neotetraose and a construction method and application thereof, and the recombinant bacillus subtilis is obtained by converting a recombinant bacillus subtilis containing beta-1, 3-N-acetyl glucosamino transferase, beta-1, 3-N-acetyl glucosamino transferase, beta-1, 3-N-acetyl glucosamino transferase and beta-1, 3-N-acetyl glucosamino transferase in bacillus subtilis 168. The recombinant plasmid is obtained by plasmids of a 1, 4-galactosyl transferase gene and a lactose permease gene. The invention provides an engineering bacterium for producing lactyl-N-neotetraose and an application of the engineering bacterium to meet the demand of the market on lactyl-N-neotetraose aiming at the huge demand of the market on lactyl-N-neotetraose and insecurity of lactyl-N-neotetraose produced by most of the existing engineering bacteria in the prior art.

The invention discloses an escherichia coli recombinant bacterium for whole-cell catalytic synthesis of rebaudioside M as well as a construction method and application of the escherichia coli recombinant bacterium. The Escherichia coli recombinant bacterium for the whole-cell catalytic synthesis of the rebaudioside M, which is constructed by the invention, contains a glycosyl transferase UGT76G1 coding gene mutant, a glycosyl transferase UGT91C1 coding gene mutant, a sucrose synthase atSuS1 gene and a polyphosphoglucokinase cgPPGK gene; a glucose-1-phosphate uridine transferase galU gene, a nucleoside diphosphate kinase ndk gene and a nucleoside diphosphate kinase ndk gene are over-expressed; meanwhile, an encoding gene araA gene of L-arabinose isomerase and an encoding gene ushA gene of 5 '-nucleotidase / UDG hydrolase are knocked out. According to the recombinant escherichia coli constructed by the invention, Reb D is prevented from being directly used as a synthetic substrate, so that the raw materials are easier to obtain, and the conversion rate of the substrate and the synthetic rate and yield of rebaudioside M are remarkably improved.

The invention belongs to the technical field of gene engineering, and particularly relates to a highland barley flavonoid glycosyl transferase gene and application thereof. A novel glycosyl transferase gene HOVUSG6091200 in highland barley is found, and protein expressed by the gene can efficiently catalyze glycosylation modification of various flavonoid compounds, so that flavonoid compound glucoside is prepared, and the health-care value of the highland barley is improved; the gene is also transferred into tobacco, so that the highland barley flavonoid glycosyl transferase gene is also expressed in a tobacco plant, flavonoid glucoside is further generated, and the value of the tobacco plant is improved; the novel gene, the recombinant vector, the recombinant bacteria and the transgenic plant provided by the invention have good application prospects.

The invention relates to the field of biology, in particular to application of a coix lacryma-jobi diacylglycerol acyltransferase gene (ClDGAT2). The invention discloses an application of a coix lacryma-jobi diacylglycerol acyltransferase gene (ClDGAT2) in improving the yield of oil. The coix lacryma-jobi diacylglycerol acyltransferase gene (ClDGAT2) has the following functions: triglyceride can be generated, a DGAT enzyme function is realized, and the capability of recovering TAG defect type saccharomyces cerevisiae oil production is realized.

The invention discloses an escherichia coli engineering strain with high benzyl acetate yield and application thereof. The escherichia coli strain efficiently expresses a brand new biosynthetic pathway related gene of benzyl acetate; comprising a phenylalanine lyase gene, a cinnamic acid coenzyme A ligase gene, a 3-hydroxyacyl coenzyme A dehydrogenase gene, a 3-oxoacyl coenzyme A ketohydrolase gene, an enoyl coenzyme A hydratase gene, a carboxylic acid reductase gene, a phosphoric acid pantetheinyl transferase gene and an acyltransferase gene. The obtained escherichia coli engineering strain can utilize a sustainable and cheap raw material glucose as a carbon source to realize efficient synthesis of benzyl acetate. The yield of benzyl acetate synthesized by the escherichia coli benzyl acetate engineering bacteria is the highest yield of benzyl acetate synthesized by a microbial fermentation method reported at home and abroad from beginning. The benzyl acetate produced by the engineering strain has the advantages of reliable quality, economic product, green production and the like, and has important economic value and social benefit.

The invention discloses a Zymomonas mobilis recombinant strain for producing D-pantothenic acid as well as a preparation method and application of the Zymomonas mobilis recombinant strain. A hydroxymethyl transferase gene panB, a ketopantoic acid reductase gene panE and a pantothenic acid synthase gene panC are integrated in a genome of the recombinant strain; an expression plasmid containing an acetolactate synthase gene Bsals, a keto acid reductoisomerase gene ilvC and a dihydroxy acid dehydratase gene ilvD is also transferred into the recombinant strain; wherein the nucleotide sequences of the gene Bsals, the gene ilvC, the gene ilvD, the gene panB, the gene panE and the gene panC are shown as SEQ ID NO. 1 to SEQ ID NO. 6 in sequence. The zymomonas mobilis ZM4 is systematically modified through a genetic engineering means, and an optimal enzyme combination adaptive to the zymomonas mobilis is defined by screening hydroxymethyltransferase, pantothenic acid synthase and ketopantoic acid reductase from different sources, so that a core foundation is laid for efficient operation of a D-pantothenic acid synthesis route, and the method has a wide application prospect. The technical bottlenecks of single metabolic pathway and low synthesis efficiency of the traditional strain are broken through.

The invention discloses glycosyltransferase in safflower and a related biological material and application thereof. The invention firstly discloses any one of the following proteins: A1) a protein consisting of an amino acid sequence as shown in a sequence 2; a2) a fusion protein obtained by connecting the N end or / and C end of the protein as shown in the sequence 2 with a protein tag. The invention further discloses the protein related biological material and application thereof. The HSYA biosynthesis key glycosyl transferase gene UGT708U5 is obtained from safflower for the first time, it is proved that the protein UGT708U5 coded by the gene can catalyze phloretin to form HSYA precursor compounds, namely phloretin-mono-C-glucoside and phloretin-di-C-glucoside, and the gene plays an important role in biosynthesis and heterologous production of HSYA; the method has important theoretical and practical significance for regulating and producing the quinonechalcone C-glycoside compounds of plants and cultivating high-quality safflower carthamus.

The invention discloses a corynebacterium glutamicum engineering strain produced by L-glutamine as well as a construction method and application of the corynebacterium glutamicum engineering strain. The corynebacterium glutamicum engineering strain is prepared by integrating a glutamine synthase encoding gene glnA which is sourced from saccharomyces cerevisiae and has a tyrosine residue mutation at the 99th site on the surface and an sRNA-MicC-EcHfq regulation module of a targeted adenylyltransferase gene glnE into a vector plasmid; the method comprises the following steps: constructing a recombinant plasmid for removing adenosine acylation of glutamine synthase; and transferring the recombinant plasmid into corynebacterium glutamicum of which the odhA gene expression is controlled by a growth stage specific promoter Pcg2705, so as to obtain a corynebacterium glutamicum engineering strain for producing L-glutamine. According to the invention, through multi-gene synergistic modification and space-time metabolic flux optimization, the problem of'growth-synthesis' tradeoff in the prior art is solved, the fermentation efficiency is improved, the fermentation process is simple, and the application prospect is good.

The present application belongs to the technical field of genetic engineering, and particularly relates to a methyltransferase gene CmCMT2 and CmDRM2 application in regulating chrysanthemum flowering. The present application constructs an RNAi silencing expression vector by isolating CmCMT2 and CmDRM2 genes in a chrysanthemum variety "Jinma", and obtains CmCMT2 -RNAi and CmDRM2 -RNAi transgenic "Jinma" plants by using a genetic transformation method, then performs phenotype observation and statistics on the transgenic plants, and explores CmCMT2 and CmDRM2 the mechanism of the genes affecting early flowering of chrysanthemum by using an RNA-seq method. The CmCMT2 and CmDRM2 genes are cloned from the chrysanthemum variety "Jinma", and spatiotemporal expression analysis shows that CmCMT2 and CmDRM2 the genes are highly expressed during the process of "Jinma" from bud development to color change; and CmCMT2 and CmDRM2 the expression level in leaves gradually decreases during the growth and development of "Jinma". The research results show that CmCMT2 and CmDRM2 the content of GA1 is changed by negatively regulating the expression of CmG20ox2a and CmG20ox2b , so that the flowering period of chrysanthemum is advanced by 15 and 8 days, respectively.

The invention discloses an engineering strain for producing uridine diphosphate, a construction method and a method for synthesizing rebaudioside M, and belongs to the technical field of gene engineering. According to the engineering strain, genes such as a pyrimidine nucleoside operon gene pyrBCAKDFE, a carbonic anhydrase gene cynT and a carbamyl phosphate synthetase gene pyrAA / ABE951 * are integrated on a genome, and genes such as an aspartate carbamyl transaminase gene pyrB, an orotic acid phosphoribose transferase gene pyrE and a phosphoribose pyrophosphate synthase gene prsD128A are deleted. The engineering strain can efficiently synthesize uridine diphosphate, and can act together with a functional protein expression recombinant strain to catalytically synthesize rebaudioside M by taking sucrose and rebaudioside A as substrates.

The invention relates to application of a gene pmtA in improving broad-spectrum tolerance of escherichia coli. According to the present invention, the research results show that the codon-optimized (derived from Rhodobacter sphaeroids) gene pmtA for coding phosphatidyl ethanolamine-N-methyltransferase EC 2.1. 1.17 is integrated to the escherichia coli genome ldhA site, such that phosphatidylcholine can be introduced to remodel the escherichia coli cell membrane phospholipid system so as to improve the broad-spectrum tolerance of escherichia coli; in addition, an artificial regulation element M1-12 and a gene pmtA are assembled to construct a tolerance module, and the tolerance module is integrated to a lactic dehydrogenase encoding gene ldhA site of an escherichia coli genome through a lambda Red homologous recombination method to obtain an engineering bacterium named + PC. According to the engineering strain provided by the invention, the broad-spectrum tolerance of the strain is improved, the methanol utilization capability of escherichia coli is remarkably enhanced while the tolerance of an intermediate toxic substrate formaldehyde is enhanced, and the TAL synthesis capability is remarkably enhanced while the tolerance to TAL is enhanced.

The invention belongs to the technical field of plant genetic engineering, and particularly discloses phlorizin glycosylation protease, a gene and application of the phlorizin glycosylation protease. According to the application, glycosyl transferase genes PGGT1.1 and PGGT1.2 for synthesizing phloretin 2 '-O-xylose glucoside through phlorizin glycosylation are obtained, the PGGT1.1 and the PGGT1.2 are over-expressed in plants, and the content of phloretin 2'-O-xylose glucoside in plant leaves is obviously increased, so that biosynthesis, separation and extraction of phloretin 2 '-O-xylose glucoside are facilitated.

The invention belongs to the technical field of gene engineering, and particularly relates to application of methyltransferase genes CmCMT2 and CmDRM2 in regulation and control of flowering of chrysanthemum. According to the invention, CmCMT2 and CmDRM2 genes in a chrysanthemum variety 'Shenma' are separated, an RNAi silent expression vector is constructed, CmCMT2-RNAi and CmDRM2-RNAi transgenic 'Shenma' plants are obtained by using a genetic transformation method, then phenotype observation and statistics are carried out on the transgenic plants, key genes influencing chrysanthemum flowering are excavated by using an RNA-seq method, and a mechanism of the CmCMT2 and CmDRM2 genes influencing chrysanthemum early flowering is explored. The CmCMT2 gene and the CmDRM2 gene are cloned from a chrysanthemum variety 'Shenma', and spatio-temporal expression analysis shows that the CmCMT2 gene and the CmDRM2 gene are highly expressed in the process from bud development to coloring of the 'Shenma'; moreover, in the growth and development process of the'miraculous horse ', the expression levels of the CmCMT2 and the CmDRM2 in leaves are gradually reduced. Research results show that the CmCMT2 and the CmDRM2 change the content of the GA1 by negatively regulating the expression of the CmG20ox2a and the CmG20ox2b, so that the florescence of the chrysanthemum is respectively advanced by 15 days and 8 days.

The invention discloses an ergothioneine-producing recombinant engineering bacterium as well as a construction method and application thereof, and belongs to the technical field of gene recombination fermentation. The method comprises the following steps: by taking escherichia coli as a starting bacterium, carrying out recombinant expression on a histidine trimethyl inner salt cysteine sulfoxide synthetase egt1 gene, an L-histidine methyltransferase egtD gene, a histidine trimethyl inner salt cysteine sulfoxide lyase egt2 gene, a methionine adenosine transferase metK gene, an adenylate kinase adK gene and an adenine phosphoribose transferase apt gene; on this basis, supply of precursor substances including histidine, cysteine and methionine is further improved through metabolic transformation, the yield of the finally obtained recombinant engineering bacterium for producing ergothioneine reaches 2.54 g / L after 48 h of shake-flask culture, the yield of a 5L fermentation tank reaches 18 g / L after further enlarged culture, the yield of ergothioneine is guaranteed while energy consumption and cost are reduced, and the method is suitable for industrial production. The method is suitable for practical popularization.

The invention discloses a salmonella typhimurium EN-VNP strain based on flagellin and LPS attenuation as well as a preparation method and application of the salmonella typhimurium EN-VNP strain, and particularly relates to the technical field of biology. The bacterial strain is formed by sequentially knocking out or silencing flagellin genes fliC and fljB, a phosphoethanolamine transferase gene eptA, a 4-amino-4-deoxy-L-arabinose transferase gene arnT and phosphotransferase yeiU on the basis of a salmonella VNP 20009 bacterial strain, and knocking in or overexpressing a phosphotransferase gene lpxE. The salmonella typhimurium EN-VNP strain disclosed by the invention has relatively high safety, the attenuated strain does not contain flagellin, and a lipid A molecule of LPS only retains one phosphate group, so that the safety of the strain is improved, and the strain is more suitable for serving as a vaccine and a drug delivery carrier.

The application belongs to the technical field of biology and relates to a Clostridium butyricum engineering strain for improving butyric acid synthesis through a coenzyme A transferase pathway as well as a construction method and application of the Clostridium butyricum engineering strain. Clostridium tyrobutyricum The application expresses a butyryl coenzyme A:acetic acid-coenzyme A transferase gene from Clostridium tyrobutyricum Ctcat1 A coenzyme A transferase (CoAT) pathway is constructed, the pathway synthesizes butyric acid by taking acetic acid as a substrate, therefore, the content of acetic acid in a fermentation product is greatly reduced, and the butyric acid yield is significantly improved.

The invention discloses application of a methyltransferase gene EFS in regulation and control of plant salt stress resistance, and belongs to the technical field of plant molecular biology. The methyltransferase gene EFS is separated from arabidopsis thaliana, the full-length CDS of the methyltransferase gene EFS is 5418bp, the methyltransferase gene EFS has three conservative protein function structural domains which are respectively an SET structural domain, an AWS structural domain and a ZnfCW structural domain, and the protein coded by the methyltransferase gene EFS contains 1805 amino acids. After the methyltransferase gene EFS is knocked out, 100mM NaCl and 125mM NaCl are used for treating a mutant, so that the sensitivity of the mutant to the salt stress is increased, which indicates that the mutant plays an important role in the response of the plant to the salt stress, and therefore, the gene can be applied to the salt stress resistance of the plant.

The invention relates to an arginyl-tRNA-protein transferase gene capable of improving the fiber length of upland cotton and application of the arginyl-tRNA-protein transferase gene, and the arginyl-tRNA-protein transferase gene related to the fiber length of the upland cotton is excavated through upland cotton variety population re-sequencing and whole genome correlation analysis, namely, ATE (arginyl-tRNA-protein transferase). Whole genome association analysis results show that the gene is closely associated with the fiber length character. By detecting that the genotype of the 1283bp site of the arginyl-tRNA-protein transferase gene is A, it can be judged that cotton is a cotton plant with high fiber length.

The present invention relates to the field of biotechnology, and discloses a dsRNA for efficiently controlling Phyllotreta striolata and its application. A dsRNA for efficiently controlling Phyllotreta striolata, the dsRNA is transcribed from an arylalkylamine N-acyltransferase gene, and the dsRNA has an RNA sequence as shown in SEQ ID NO.1. The arylalkylamine N-acyltransferase gene sequence is as shown in SEQ ID NO.2. By using the dsRNA for efficiently controlling Phyllotreta striolata and its application as described above, the provided target gene, the arylalkylamine N-acyltransferase gene, has specificity and is highly expressed in the digestive tissues of adult Phyllotreta striolata, which can reduce the difficulty of the dsRNA reaching the target tissue, improve the interference effect of the dsRNA on Phyllotreta striolata, and further improve the control effect of the dsRNA on Phyllotreta striolata.

The invention provides glycosyl transferase and application thereof in preparation of salidroside, and relates to the technical field of enzyme engineering. The glycosyl transferase FpUGT provided by the invention is derived from fraxinus pennsylvanica, the existence of the glycosyl transferase gene capable of directly catalytically synthesizing the salidroside in the plant is not reported in literatures, and a new biological way is provided for finding the glycosyl transferase for efficiently synthesizing the salidroside. And a new biological material is provided for preparing the salidroside.

This invention provides a chalcone isopentenyltransferase gene. GiPT16 This invention relates to the GiPT16 protein, amplification primer set, and applications, belonging to the field of biogenetics technology. It is based on *Glycyrrhiza inflata* (GiPT16 protein, amplification primer set, and applications). Glycyrrhiza inflata Using whole-genome sequencing data and a reverse genetics strategy, the key aromatic isopentenyltransferase GiPT16, involved in the isopentenylation modification of chalcone active ingredients, was successfully identified and functionally characterized. This enzyme was confirmed to specifically catalyze the biosynthesis of psoralen and glycyrrhizin C using DMAPP as a donor. It is the first chalcone isopentenyltransferase characterized in *Glycyrrhiza inflata*, filling a gap in the study of key enzymes in the chalcone isopentenylation metabolic pathway of this species and providing important evidence for further elucidating the molecular mechanisms of quality formation in *Glycyrrhiza inflata* medicinal materials.