77 results about "Transferase Gene" patented technology

The present application belongs to the technical field of genetic engineering, and particularly relates to a methyltransferase gene CmCMT2 and CmDRM2 application in regulating chrysanthemum flowering. The present application constructs an RNAi silencing expression vector by isolating CmCMT2 and CmDRM2 genes in a chrysanthemum variety "Jinma", and obtains CmCMT2 -RNAi and CmDRM2 -RNAi transgenic "Jinma" plants by using a genetic transformation method, then performs phenotype observation and statistics on the transgenic plants, and explores CmCMT2 and CmDRM2 the mechanism of the genes affecting early flowering of chrysanthemum by using an RNA-seq method. The CmCMT2 and CmDRM2 genes are cloned from the chrysanthemum variety "Jinma", and spatiotemporal expression analysis shows that CmCMT2 and CmDRM2 the genes are highly expressed during the process of "Jinma" from bud development to color change; and CmCMT2 and CmDRM2 the expression level in leaves gradually decreases during the growth and development of "Jinma". The research results show that CmCMT2 and CmDRM2 the content of GA1 is changed by negatively regulating the expression of CmG20ox2a and CmG20ox2b , so that the flowering period of chrysanthemum is advanced by 15 and 8 days, respectively.

The invention discloses an ergothioneine-producing recombinant engineering bacterium as well as a construction method and application thereof, and belongs to the technical field of gene recombination fermentation. The method comprises the following steps: by taking escherichia coli as a starting bacterium, carrying out recombinant expression on a histidine trimethyl inner salt cysteine sulfoxide synthetase egt1 gene, an L-histidine methyltransferase egtD gene, a histidine trimethyl inner salt cysteine sulfoxide lyase egt2 gene, a methionine adenosine transferase metK gene, an adenylate kinase adK gene and an adenine phosphoribose transferase apt gene; on this basis, supply of precursor substances including histidine, cysteine and methionine is further improved through metabolic transformation, the yield of the finally obtained recombinant engineering bacterium for producing ergothioneine reaches 2.54 g / L after 48 h of shake-flask culture, the yield of a 5L fermentation tank reaches 18 g / L after further enlarged culture, the yield of ergothioneine is guaranteed while energy consumption and cost are reduced, and the method is suitable for industrial production. The method is suitable for practical popularization.

The application belongs to the technical field of biology and relates to a Clostridium butyricum engineering strain for improving butyric acid synthesis through a coenzyme A transferase pathway as well as a construction method and application of the Clostridium butyricum engineering strain. Clostridium tyrobutyricum The application expresses a butyryl coenzyme A:acetic acid-coenzyme A transferase gene from Clostridium tyrobutyricum Ctcat1 A coenzyme A transferase (CoAT) pathway is constructed, the pathway synthesizes butyric acid by taking acetic acid as a substrate, therefore, the content of acetic acid in a fermentation product is greatly reduced, and the butyric acid yield is significantly improved.

The invention provides glycosyl transferase and application thereof in preparation of salidroside, and relates to the technical field of enzyme engineering. The glycosyl transferase FpUGT provided by the invention is derived from fraxinus pennsylvanica, the existence of the glycosyl transferase gene capable of directly catalytically synthesizing the salidroside in the plant is not reported in literatures, and a new biological way is provided for finding the glycosyl transferase for efficiently synthesizing the salidroside. And a new biological material is provided for preparing the salidroside.

This invention provides a chalcone isopentenyltransferase gene. GiPT16 This invention relates to the GiPT16 protein, amplification primer set, and applications, belonging to the field of biogenetics technology. It is based on *Glycyrrhiza inflata* (GiPT16 protein, amplification primer set, and applications). Glycyrrhiza inflata Using whole-genome sequencing data and a reverse genetics strategy, the key aromatic isopentenyltransferase GiPT16, involved in the isopentenylation modification of chalcone active ingredients, was successfully identified and functionally characterized. This enzyme was confirmed to specifically catalyze the biosynthesis of psoralen and glycyrrhizin C using DMAPP as a donor. It is the first chalcone isopentenyltransferase characterized in *Glycyrrhiza inflata*, filling a gap in the study of key enzymes in the chalcone isopentenylation metabolic pathway of this species and providing important evidence for further elucidating the molecular mechanisms of quality formation in *Glycyrrhiza inflata* medicinal materials.

The application provides a kind of aromatic isopentenyl transferase mutant, which is based on the aromatic isopentenyl transferase gene MenA derived from Bacillus subtilis ( Bacillus subtilis ) and six aromatic isopentenyl transferase mutants MenA-1 to MenA-6 are obtained by site-directed mutagenesis PCR technology, and the recombinant bacteria Bacillus subtilis expressing the above mutants are constructed, respectively. The yield of heptamethylnaphthoquinone fermented by the recombinant bacteria is 64.49 mg / L, 69.30 mg / L, 83.86 mg / L, 105.84 mg / L, 119.23 mg / L and 125.45 mg / L, respectively, which is 133%, 143%, 173%, 219%, 246% and 259% of the yield of heptamethylnaphthoquinone fermented by the Bacillus subtilis expressing the wild-type aromatic isopentenyl transferase MenA.

This invention belongs to the field of biotechnology and discloses a recombinant strain of Saccharomyces cerevisiae that enhances the efficient synthesis of vitamin K2 and its application. By heterologously integrating the hepS / T and naphthoquinone cyclotransferase genes (menA) of Bacillus subtilis, four genetically engineered strains were constructed based on the designed MK-7 biosynthetic pathway: YK-1, YK-2, YK-3, and YK-4. YK-1 is an expression strain containing the target genes MenF, MenD, and MenH; YK-2 is an expression strain integrating the target genes MenC, MenE, and MenB on the basis of YK-1; YK-3 is an expression strain integrating the target genes hepS and hepT on the basis of YK-2; and YK-4 is an expression strain integrating the target genes yuxO, MenA, and MenG on the basis of YK-3. YK-4 is the final engineered strain that produces high levels of vitamin K2. The optimal fermentation time was determined by measuring OD600 and yield curves. YK-4 reached its maximum cell concentration in 18 hours, which can effectively shorten the fermentation cycle.

The invention discloses a genetically engineered bacterium for synthesizing N-acetyl blue as well as a construction method and application of the genetically engineered bacterium. The genetically engineered bacterium is prepared by knocking out a lactic dehydrogenase gene ldh and inserting a phosphoketolase gene xfpK on a knockout site, knocking out a pyruvate oxidase gene poxB and inserting a phosphate transacetylase gene pta on a knockout site of a microorganism for producing N-acetyl blue, and knocking out an aconitase inhibition factor gene acnR, inserting a glutamate dehydrogenase gene gdhA on a knockout site, knocking out a glutamine synthetase adenylation transferase gene glnE, and inserting an alpha-ketoglutarate dehydrogenase inhibition factor gene odhI on a knockout site to obtain the strain. According to the method, the supply of the precursor acetyl coenzyme A is enhanced by introducing a non-oxidative glycolysis pathway, the pH dynamic regulation system balances the metabolic flux, and the double breakthrough of the yield and the carbon recovery rate of the N-acetyl blue is realized by combining a two-stage fermentation process.

This invention belongs to the fields of fermentation and genetic engineering technology, and provides a high-yield hexanoic acid-producing engineered *Escherichia coli* strain, its construction method, and its applications. This engineered strain is *Escherichia coli*... E. coli w3110 was the starting strain, overexpressing the acetyl-CoA transferase gene. act and β-ketothiolase gene bktB Overexpression of trans-enoyl-CoA reductase gene ter 3-Hydroxybutyryl-CoA dehydrogenase gene hbd, and 3-hydroxybutyryl coenzyme A dehydratase gene crt Or, simultaneously not expressing the pyruvate formate lyase gene. pflB or lactate dehydrogenase gene ldhA This engineered bacteria can convert glucose into hexanoic acid, a high-value product, with a yield of 4.968 g / L after 18 hours of fermentation. It adopts aerobic fermentation, resulting in rapid cell growth, a short fermentation cycle, and a high acid production rate. The fermentation process is simple, easy to control, and has low production costs, which is conducive to its promotion and application in industrial production.

The invention discloses genetically engineered corynebacterium glutamicum and an application of the genetically engineered corynebacterium glutamicum in preparation of ectoine, and the genetically engineered corynebacterium glutamicum contains an L-diaminobutyric acid transaminase gene ectB, an L-diaminobutyric acid acetyltransferase gene ectA and an ectoine synthetase gene ectC, the invention relates to an aspartic acid transferase gene aspB, an aspartic acid kinase gene lysC and an aspartic acid semialdehyde dehydrogenase gene asd, wherein the nucleotide sequences of the gene aspB, the gene lysC and the gene asd are respectively as shown in SEQ ID NO.4, SEQ ID NO.5 and SEQ ID NO.6. The invention further discloses a preparation method of the aspartic acid transferase gene aspB, the aspartic acid kinase gene lysC and the aspartic acid semialdehyde dehydrogenase gene asd. After the recombinant corynebacterium glutamicum constructed by the invention is fermented for 60 hours, the yield of ectoine can reach 50g / L. The genetically engineered corynebacterium glutamicum disclosed by the invention has important practical significance on industrial production and large-scale application of ectoine.

The invention discloses a recombinant bacterium for producing mevalonic acid by taking glucose and acetone as co-substrates as well as a construction method and application of the recombinant bacterium, and belongs to the technical field of genetic engineering. The problems that an existing mevalonic acid biosynthesis mode is low in carbon atom economy, limited in theoretical yield and the like are solved. According to the invention, acetone carboxylase, acetoacetyl coenzyme A synthetase, 3-hydroxy-3-methylglutaryl coenzyme A reductase and hydroxymethyl glutaryl-CoA synthetase are subjected to heterologous expression in escherichia coli with an acetyl coenzyme A acetyltransferase gene atoB and a histidine protein kinase gene atoS knocked out, and self carbonic anhydrase is over-expressed; and a new way for synthesizing MVA by using glucose and acetone as a co-substrate is constructed. According to the method, one molecule of CO2 can be fixed in the biosynthesis of mevalonic acid, the yield of carbon atoms in the synthesis process of mevalonic acid is increased, the problem of low yield of carbon atoms in the synthesis process of mevalonic acid by taking glucose as a single substrate is solved, and a new thought is provided for constructing a carbon neutralization type biological manufacturing technology.

The invention belongs to the technical field of biology, and particularly relates to flavonoid glycosyl transferase and application thereof. The invention relates to a flavonoid glycosyl transferase gene, wherein the nucleotide sequence of the flavonoid glycosyl transferase gene is shown as SEQ ID NO. 1. By focusing the rice drought resistance related gene and predicting and coding the flavonoid glycosyl transferase, through corresponding experiments, key biochemical evidences are provided for clarification of a molecular mechanism of the rice drought resistance related gene in drought response, a foundation is laid for analysis of a mechanism of glycosylation modification regulation and control of rice drought resistance, and the application of the flavonoid glycosyl transferase to rice drought resistance regulation and control is developed. The method has important theoretical value and application potential for crop stress resistance genetic improvement.

The invention relates to a method for preparing irisflorentin by utilizing belamcandin, and belongs to the technical field of biology. The method comprises the following steps: carrying out C-5 site and C-3'site methylation reaction by taking white belamcandin as a substrate, taking belamcanda methyl transferase gene BcOMT03 gene encoding protein and belamcanda methyl transferase BcOMT33 gene encoding protein as catalysts and taking S-adenosine-methionine as a methyl donor to generate a corresponding glycosylation product. According to the invention, the functions of the two methyltransferase genes BcOMT03 and BcOMT33 in the blackberry lily are identified and verified for the first time, the effect of the two methyltransferase genes BcOMT03 and BcOMT33 in catalyzing the ibullamcandin to produce the irisflorentin is defined, and the blank of research on the biosynthetic pathway of the characteristic component irisflorentin of the blackberry lily is filled up; important information is provided for research on a biosynthesis mechanism of active ingredients of medicinal plants.

The application belongs to the technical field of genetic engineering, and discloses a kind of synthetic lactosyl-N-neotetraose and safe recombinant bacillus subtilis and its construction method and application, the recombinant bacillus subtilis is obtained by transforming plasmid containing beta-1,3-N-acetylglucosamine transferase, beta-1,4-galactosyltransferase gene and lactose permease gene in bacillus subtilis 168.The application aims at the huge demand of lactosyl-N-neotetraose in the market in prior art, and the lactosyl-N-neotetraose produced by most existing engineering bacteria is not safe, provides an engineering bacteria for producing lactosyl-N-neotetraose and application, to meet the demand of lactosyl-N-tetraose in the market.

The application belongs to the technical field of genetic engineering and enzyme engineering, and discloses a dragon blood tree flavone compound O-methyltransferase gene and application thereof. Three functionally differentiated O-methyltransferase genes DcOMT1, DcOMT2 and DcOMT3 are cloned from dragon blood tree, and the coded proteins can catalyze O-methylation reaction of flavonoids. The three enzymes show different catalytic modes for dracorhodin D: DcOMT1 preferentially catalyzes 4,4'-dihydroxy-2,6-dimethoxydihydrochalcone to generate dracorhodin B and further catalyzes dracorhodin B; DcOMT2 catalyzes dracorhodin B; and DcOMT3 catalyzes 4,4'-dihydroxy-2,6-dimethoxydihydrochalcone. The functions of the three enzymes are complementary, and can be used alone or in combination to realize precise synthesis of multiple different methylated flavones from a single substrate.

The invention relates to the field of plant genetic engineering, in particular to application of ginseng glycosyl transferase UGT84K2 to improvement of abiotic stress resistance of plants. The ginseng glycosyl transferase UGT84K2 gene is transferred into a model plant arabidopsis thaliana for research, an overexpression strain of the ginseng glycosyl transferase UGT84K2 is constructed, salt-resistant and drought-resistant adversity stress treatment is performed, and results show that under the conditions of adversity high salt (NaCl), drought stress (osmotic stress) and the like, the expression quantity of the ginseng glycosyl transferase UGT84K2 is up-regulated, and the expression quantity of the ginseng glycosyl transferase UGT84K2 is up-regulated. And the ginseng glycosyl transferase UGT84K2 overexpression strain shows stronger tolerance under the adverse condition, which reflects that the stress resistance of the ginseng glycosyl transferase UGT84K2 overexpression strain can be enhanced through glycosylation modification.

The invention belongs to the technical field of genetic engineering, and discloses a genetically engineered bacterium for efficiently synthesizing gamma-aminobutyric acid from the beginning by efficiently utilizing a mixed carbon source, a method and application, and the genetically engineered bacterium is obtained by overexpressing edd from Zymomonas mobilis on the basis of escherichia coli E. coli G16; eda from the zymomonas mobilis is over-expressed; performing overexpression on acs (Acetobacter pasteurianus) sources; a malic acid synthase gene aceB is knocked out; a malic acid synthase gene glcB is knocked out; and the acetyl phosphate transferase gene eutD is knocked out. The escherichia coli strain which is clear in genetic background and capable of producing gamma-aminobutyric acid is utilized, and an ED path and an acetic acid utilization path are introduced, so that acetyl CoA branch metabolism is reduced, and the yield and conversion rate of gamma-aminobutyric acid are remarkably improved.

The invention discloses application of an epiregulatory factor gene in regulation of plant heat resistance. The epiregulatory factor gene (TaMET) is identified from a wheat genome for the first time. The gene is subjected to transient overexpression in wheat Fielder, and it is found that wheat can be more sensitive to heat expression. The homologous gene (OsMET) of the gene in rice is subjected to conservative structural domain analysis to find that the gene is an SAM dependent type methylated transferase gene, and the modification level of histone H3K27me3 can be influenced. Research finds that overexpression of the gene makes the plant more sensitive to heat, knockout of the gene can significantly improve the heat resistance of rice Nipponbare, that is, the gene belongs to a negative regulation factor of rice heat resistance, for example, deep analysis of a mechanism of the gene or a homologous gene thereof is beneficial to excavation of heat-resistant genes in rice and wheat.

This invention belongs to the field of plant biocontrol. Specifically, this invention provides the application of the rice RNA adenine methyltransferase EDM2L gene in regulating plant antiviral resistance. This was achieved by comparing rice plants inoculated with RSV virus. edm2l Knockout mutants and edm2l' Analysis of methyltransferase catalytic activity mutants and ZH11 wild plants revealed that knockout edm2l Downregulation of EDM2L expression significantly weakens rice's resistance to RSV, indicating that this gene has an anti-RSV function. This invention provides a new target gene locus for cultivating crops with enhanced virus resistance using genetic engineering techniques, and has significant implications and broad application prospects in regulating plant resistance and disease-resistant breeding.

The application belongs to the technical field of biology and particularly relates to a flavonoid glycosyltransferase and application thereof. A flavonoid glycosyltransferase gene, wherein the nucleotide sequence of the flavonoid glycosyltransferase gene is shown as SEQ ID NO. 1. The application focuses on drought-resistant related genes of rice, predicts that a flavonoid glycosyltransferase is coded, and through corresponding experiments, not only provides key biochemical evidence for elucidating the molecular mechanism of drought-resistant related genes of rice in drought response, but also lays a foundation for analyzing the mechanism of glycosylation modification regulating drought resistance of rice, has important theoretical value and application potential for genetic improvement of crop stress resistance.

The invention relates to application of cyanobacteria acetyltransferase in increasing the content of astaxanthin in escherichia coli for producing astaxanthin, and also provides a method for increasing the content of astaxanthin in escherichia coli for producing astaxanthin, which comprises the step of introducing a cyanobacteria acetyltransferase gene expression cassette into escherichia coli. The method comprises the following steps: amplifying a target gene cKAT from a genome of blue-green algae, inserting the cKAT into an escherichia coli expression vector by using a recombinant DNA technology, and transforming the constructed expression vector into an escherichia coli BW-ASTA strain to obtain recombinant escherichia coli. Therefore, the content of the astaxanthin in the escherichia coli is increased by about 4 times, and a novel efficient, stable and large-scale microbial synthesis scheme is provided for greatly reducing the production cost of the astaxanthin.

This invention provides a genetically engineered *Yersinia lipolytica* strain and its applications. The genetically engineered strain is a *Yersinia lipolytica* Po1f strain with the PEX10 gene knocked out, expressing the C16 / 18 elongase gene MaELO3 from *Morchella alpina*, the elongase gene AtKCS from *Arabidopsis thaliana*, and the elongase gene CraKCS from *Brassica oleracea*. The strain also expresses the elongase gene CgKCS from *Capsella*. Further expression of the desaturase gene MaD15D from *Morchella alpina*, and overexpression of the strain's own diacylglycerol transferase gene DGA1 and its own Δ9 desaturase gene OLE1, resulted in a nervonic acid yield of 111.6 mg / L in shake-flask fermentation. By adding 1% rapeseed oil exogenously, the nervonic acid yield reached 185.1 mg / L. The *Yersinia lipolytica* genetically engineered strain constructed in this invention is simple to operate, has stable and reliable performance, and can be applied to large-scale commercial production.

The invention relates to the technical field of bioengineering, and discloses a construction method and application of recombinant Escherichia coli engineering bacteria with high yield of ergothioneine, and the method comprises the following steps: by taking Escherichia coli as a chassis, integrating a mycobacterium smegmatis egtABCDE gene cluster at an ldhA site; a repressor protein gene metJ and a cysteine lyase gene yhaM are knocked out, a methionine adenosine transferase gene metK and a serine acetyltransferase gene cysE are overexpressed by using a strong promoter, and mutant metA, thrA, serA and hisG genes for relieving feedback inhibition are introduced, so that metabolic inhibition of methionine, cysteine and histidine pathways is systematically relieved; the invention also establishes a matched fermentation process. According to the invention, endogenous precursor supply and exogenous synthesis pathways are synergistically enhanced, the precursor supply bottleneck is solved, the yield of ergothioneine is increased, and the method is suitable for industrial production.

The invention relates to a dactylicapnos root O-methyltransferase DsOMT018 gene and application thereof, and belongs to the technical field of biology. The nucleotide sequence of the DsOMT018 gene is shown as SEQ ID NO.1, the amino acid sequence of the encoded protein of the DsOMT018 gene is shown as SEQ ID NO.2, and the DsOMT018 gene can be used for preparing isocorydine and corydine. The separation and identification of the DsOMT018 not only breaks through the technical bottleneck of non-model plant gene mining, but also provides a new normal form for sustainable manufacturing of high-added-value alkaloids through full-chain innovation of'precise catalysis-metabolic network-green production '. The advantages of the isocorydine and corydine in synthesis of isocorydine and corydine can promote the leap-wise development of the fields of natural medicine development and synthetic biology.

The application provides a phloridzin glycosyltransferase gene in dendrobium officinale DoUGT885 and application thereof, and belongs to the technical field of gene cloning. Based on the dendrobium officinale genome data, a key enzyme for synthesizing phloridzin, i.e. a phloridzin glycosyltransferase gene, is successfully explored and identified by a reverse genetics method DoUGT885 , and it is confirmed that DoUGT885 the glycosyltransferase can specifically catalyze the glycosylation of phloridzin to generate phloridzin, which proves that the glycosyltransferase can specifically catalyze the glycosylation of phloridzin to generate phloridzin, and solves the problem of the scarcity of high-efficiency and specific glycosyltransferase elements in the biosynthesis of phloridzin. The discovery of the enzyme breaks the source limitation of the existing phloridzin synthesis enzyme, provides a new high-quality enzyme element selection for the biosynthesis system, not only enriches the glycosyltransferase resource library, but also reduces the dependence of phloridzin biosynthesis on traditional enzyme elements, and lays a key foundation for constructing an efficient and stable phloridzin biosynthesis pathway.

The application discloses application of a tobacco zeatin glucosyltransferase gene in regulating tobacco seed germination time. The gene is NtZOG, the nucleotide sequence of which is shown as SEQ ID NO. 1, and the amino acid sequence of which is shown as SEQ ID NO. 2. Research finds that by knocking out the tobacco zeatin glucosyltransferase gene NtZOG from wild tobacco K326, a tobacco mutant with the NtZOG gene knocked out is constructed. By comparing the germination of the wild tobacco K326 and the mutant, it is found that the germination rate of the mutant is significantly lower than that of the wild tobacco at 4.5 days of development, and is as low as about 50%, so it can be seen that the tobacco zeatin glucosyltransferase gene NtZOG has an important regulating effect on tobacco seed germination, and the expression of the gene can be regulated to regulate the germination time of tobacco, and the application has a potential application prospect in the tobacco planting field.

The present disclosure relates to a method to reduce virulence of Tsukamurella comprising decreasing the expression of the mycolyltransferase C (‘tmytC”) gene. Also disclosed is a pharmaceutical composition and method of prevention and treatment of infection by inhibition of tmytC.

The invention relates to a dactylicapnos root O-methyltransferase DsOMT014 gene and application thereof, and belongs to the technical field of biology. The nucleotide sequence of the dactylicapnos root O-methyltransferase DsOMT014 gene is shown as SEQ ID NO.1, the amino acid sequence of the encoded protein of the dactylicapnos root O-methyltransferase DsOMT014 gene is shown as SEQ ID NO.2, and the dactylicapnos root O-methyltransferase DsOMT014 gene can be The method is a key step for synthesizing the isoquinoline alkaloid in the dactylicapnos scandens and is a key node for regulating and controlling a metabolic pathway, and functional analysis of the DsOMT014 provides a key clue for understanding biosynthesis logic of the dactylicapnos scandens alkaloid.

The invention discloses O-methyltransferase, a gene, a mutant, a fusion enzyme and application of the O-methyltransferase, and belongs to the technical field of bioengineering. The O-methyltransferase SmOMT has substrate mixing property and regioselectivity, and the amino acid sequence of the O-methyltransferase SmOMT is as shown in SEQ ID NO. 1. The mutant SmOMTM2 is obtained by carrying out double mutation of E152A and I306A on the basis of SmOMT, so that the mutant SmOMTM2 has higher catalytic activity. Meanwhile, the invention constructs an SAM independent methylation system, and the system comprises a fusion enzyme AtHMTV140T-L95-SmOMTM2 which is formed by the mutant SmOMTM2 and a mutant halide methyltransferase AtHMTV140T, and the fusion enzyme AtHMTV140T-L95-SmOMTM2 can be used for preparing the SAM. The fusion enzyme can realize methylation of various substrates without exogenous SAM, has higher catalytic efficiency than a free enzyme cascade system, and can be used for synthesizing methylated compounds with enhanced anti-inflammatory activity.

The invention provides UDP-glucose transferase for catalyzing glycosylation at C2'site of aescin, a gene, a primer group and application, and belongs to the technical field of aescin. The method is based on aesculus chinensis genome and transcriptome data analysis; according to the present invention, the UGT gene for catalyzing the C2'site of the protoaescinogenin-3-O-beta-D-glucopyranuronide is excavated, and the tobacco transient expression system is utilized to verify that the AcUGT94AK1, the AcUGT94AK3 and the AcUGT94AK6 can catalyze the protoaescinogenin-3-O-beta-D-glucopyranuronide to form the protoaescinogenin-3-O-beta-D-glucopyranuronide-(1-> 2)-beta-D-glucoside, such that the UGT gene can be used for catalyzing the C2 'site of the protoaescinogenin-3-O-beta-D-glucopyranuronide; the invention not only provides an important gene element for biosynthesis of aescin compounds, but also provides a key gene locus for molecular breeding of aesculus chinensis, and provides a theoretical basis for glycosylation modification of other triterpenoid saponins.